Rh2BG193400

Domain of unknown function (DUF4216)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000178
Physical Location & Seq
Reverse (-)
17504688 .. 17512228
7541 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG193400.1

Sequence Viewer

Length: 843 bp
ATGTACCTGAAGAGGCTTGGTGATGCTGGCTATAGTACCTTCAAGCTTGCCCGAACTAACAACCGTGGAGATGGAGCGAACAAGTCTCAATATCCAATTTACAAAAGAAGTCTTAAGAAGGTTCAACAGATGCAAGTTGAGGGTTCAACAGAAGATGTTGAATCCTTACTATACTTGGCCAGTGGACCTCAACGTGAAGTGGCACGATATAGTGGGTGTATCGTCAATGGCATAAGGTTTCATACTCAGAAACGCGATGCAAATAAAAAAACTCAAAACTATGGAGTGGTAGTCAAAGGAGAGCATCGTGGTAAAAGTATCGACTTTTATGGTGTTTTGAAAGATATAATTGTGTTATCATACCTTGGAAATAATCAGGTGGTCGTTTTCAAATGTGATTGGCTGGATCTCAATGCAAGAAGGGGAATTCAAGTTGATGAGAACCAGTTTACAAGTGTTAATTTTACCAAAAAATGGTATATGAATGATCCATTTGCATTGGCATGTCAAACACAGCAGGTATACTATTTGAAAGATACAAAGCATGGTAGTAATTGGCGTGTTGTAGAAAGGTCGCAGCCTAGAGGGATGTATGATTTTACAGAAAAAGAAACTGAAGTGGGTGATTCATTAGAGGAGGTAGAAGATCCATATCAGCAAGAGTCACATGGGTATGTCGATTCAGTTGAAGTTGATGTTGGAGAGACGTCATTACATAGAGATGACATGGATATGATCGTTGTTGATTTGAATGCTAAAGATAGTGATGCAGAAGATGGCGACTTTAATGATGAAGATAATGAGTTGATGTCTTATGATGATATCGAGGACGACTCTACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218

Protein Analysis

280

Amino Acids

32.12

Weight (kDa)

4.77

Isoelectric Point (pI)

30.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4216 PF13952 119 - 189 6.6e-24 Domain of unknown function (DUF4216)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G40087 AT3G30200
fragaria_vesca FvH4_1g23271 FvH4_3g22812 FvH4_3g22813 FvH4_3g31042 FvH4_3g31043 FvH4_7g03653
malus_domestica MD05G1321200.v1.1 MD09G1027200.v1.1 MD15G1434900.v1.1 MD15G1435000.v1.1 MD15G1435100.v1.1
prunus_persica Prupe.1G578800_v2.0.a1 Prupe.1G578800_v2.0.a1 Prupe.1G578800_v2.0.a1 Prupe.2G062700_v2.0.a1 Prupe.5G033400_v2.0.a1 Prupe.7G022900_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1
pyrus_communis pycom03g07600 pycom03g11360 pycom05g02870 pycom111g02130 pycom15g38370 pycom15g38380
rosa_chinensis RchiOBHm_Chr1g0346371 RchiOBHm_Chr4g0402051 RchiOBHm_Chr4g0414991 RchiOBHm_Chr5g0051011 RchiOBHm_Chr5g0051181 RchiOBHm_Chr6g0273071 RchiOBHm_Chr7g0218301 RchiOBHm_Chr7g0241081
rosa_laevigata RLG00000007709 RLG00000008953 RLG00000012400 RLG00000012401 RLG00000013805 RLG00000014199 RLG00000014726 RLG00000017792 RLG00000017793 RLG00000018860 RLG00000020749 RLG00000030708 RLG00000033507
rosa_multiflora Rmu_co8373761.1_g000001 Rmu_sc0000028.1_g000023 Rmu_sc0000235.1_g000040 Rmu_sc0000293.1_g000017 Rmu_sc0000293.1_g000019 Rmu_sc0000651.1_g000012 Rmu_sc0000870.1_g000051 Rmu_sc0001167.1_g000031 Rmu_sc0001373.1_g000039 Rmu_sc0001861.1_g000055 Rmu_sc0001891.1_g000038 Rmu_sc0001909.1_g000015 Rmu_sc0002500.1_g000002 Rmu_sc0002868.1_g000036 Rmu_sc0002868.1_g000038 Rmu_sc0002868.1_g000039 Rmu_sc0003379.1_g000001 Rmu_sc0003778.1_g000005 Rmu_sc0003778.1_g000006 Rmu_sc0010475.1_g000011 Rmu_sc0015271.1_g000002 Rmu_sc0015271.1_g000003 Rmu_sc0017962.1_g000001 Rmu_sc0017962.1_g000003 Rmu_sc0022129.1_g000004 Rmu_sc0025694.1_g000003 Rmu_sc0025694.1_g000004
rosa_roxburghii Rroxscaffold_1G00031640 Rroxscaffold_1G00047620 Rroxscaffold_1G00047630 Rroxscaffold_1G00050560 Rroxscaffold_1G00050570 Rroxscaffold_1G00054800 Rroxscaffold_1G00055890 Rroxscaffold_2G00108230 Rroxscaffold_3G00240860 Rroxscaffold_4G00299670 Rroxscaffold_4G00299680 Rroxscaffold_4G00299690 Rroxscaffold_4G00306320 Rroxscaffold_4G00306340 Rroxscaffold_5G00341060 Rroxscaffold_5G00342000 Rroxscaffold_5G00355660 Rroxscaffold_5G00357170 Rroxscaffold_6G00399490 Rroxscaffold_6G00408400 Rroxscaffold_7G00189260 Rroxscaffold_7G00199620 Rroxscaffold_7G00215470
rosa_rugosa Rorug02G0117600 Rorug02G0117700 Rorug04G0116400 Rorug06G0103200
rosa_samantha Rh1AG062800 Rh1AG073700 Rh1CG091700 Rh1DG077600 Rh1DG077700 Rh1DG077800 Rh1DG117400 Rh1DG146300 Rh2BG193300 Rh2BG193400 Rh2BG265300 Rh2BG413400 Rh3CG365500 Rh4AG315400 Rh4BG323100 Rh4BG323200 Rh4CG071400 Rh4CG142500 Rh4CG203300 Rh4CG338300 Rh4DG079300 Rh4DG079400 Rh4DG217000 Rh4DG217100 Rh4DG217200 Rh4DG217300 Rh5AG128300 Rh5AG239900 Rh5AG465900 Rh5BG348600 Rh5BG387900 Rh5BG388000 Rh5CG181800 Rh5CG181900 Rh5CG271000 Rh5CG271100 Rh5DG127100 Rh5DG138400 Rh5DG168200 Rh5DG168300 Rh5DG361500 Rh5DG460400 Rh6AG045400 Rh6AG058600 Rh6BG275000 Rh6CG089700 Rh6DG182300 Rh6DG182400 Rh6DG207300 Rh6DG207400 Rh7BG374000 Rh7BG390600 Rh7BG390700 Rh7BG390800
rosa_wichuraiana Rw0G018690 Rw1G007320 Rw4G008510 Rw5G011510 Rw5G021830 Rw6G024150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 710
Acc36I ACCTGC 1 cut(s) 508
AccB7I CCANNNNNTGG 1 cut(s) 474
AccI GTMKAC 1 cut(s) 522
AccII CGCG 1 cut(s) 255
AclWI GGATC 3 cut(s) 414, 482, 641
AcoI YGGCCR 1 cut(s) 177
AcsI RAATTY 1 cut(s) 426
AcuI CTGAAG 2 cut(s) 29, 636
AcyI GRCGYC 1 cut(s) 707
AfaI GTAC 2 cut(s) 5, 37
AfiI CCNNNNNNNGG 1 cut(s) 474
AflII CTTAAG 1 cut(s) 113
AjuI GAANNNNNNNTTGG 2 cut(s) 681, 713
AluBI AGCT 1 cut(s) 46
AluI AGCT 1 cut(s) 46
Alw26I GTCTC 2 cut(s) 90, 698
AlwI GGATC 3 cut(s) 414, 482, 641
AoxI GGCC 1 cut(s) 177
ApeKI GCWGC 1 cut(s) 577
ApoI RAATTY 1 cut(s) 426
AspS9I GGNCC 1 cut(s) 185
AsuHPI GGTGA 2 cut(s) 32, 635
AvaII GGWCC 1 cut(s) 185
BalI TGGCCA 1 cut(s) 179
BbvI GCAGC 1 cut(s) 589
BccI CCATC 2 cut(s) 65, 770
BcoDI GTCTC 2 cut(s) 90, 698
BfaI CTAG 1 cut(s) 582
BfmI CTRYAG 1 cut(s) 31
BfrI CTTAAG 1 cut(s) 113
BfuAI ACCTGC 1 cut(s) 508
BisI GCNGC 1 cut(s) 578
BlsI GCNGC 1 cut(s) 579
Bme18I GGWCC 1 cut(s) 185
BmgT120I GGNCC 1 cut(s) 185
BmsI GCATC 5 cut(s) 13, 120, 247, 313, 757
BplI GAGNNNNNCTC 1 cut(s) 818
BsaAI YACGTR 1 cut(s) 840
BsaBI GATNNNNATC 1 cut(s) 651
BsaHI GRCGYC 1 cut(s) 707
BsaJI CCNNGG 2 cut(s) 64, 364
BsaXI ACNNNNNCTCC 2 cut(s) 276, 306
Bsc4I CCNNNNNNNGG 1 cut(s) 474
Bse1I ACTGG 2 cut(s) 180, 445
Bse8I GATNNNNATC 1 cut(s) 651
BseDI CCNNGG 2 cut(s) 64, 364
BseGI GGATG 1 cut(s) 594
BseJI GATNNNNATC 1 cut(s) 651
BseLI CCNNNNNNNGG 1 cut(s) 474
BseMII CTCAG 1 cut(s) 260
BseNI ACTGG 2 cut(s) 180, 445
BseRI GAGGAG 1 cut(s) 650
BseXI GCAGC 1 cut(s) 589
Bsh1236I CGCG 1 cut(s) 255
BshFI GGCC 1 cut(s) 179
BslI CCNNNNNNNGG 1 cut(s) 474
BsmAI GTCTC 2 cut(s) 90, 698
BsmBI CGTCTC 1 cut(s) 698
BsmI GAATGC 1 cut(s) 757
BsnI GGCC 1 cut(s) 179
Bsp143I GATC 4 cut(s) 406, 487, 646, 735
BspANI GGCC 1 cut(s) 179
BspCNI CTCAG 1 cut(s) 259
BspFNI CGCG 1 cut(s) 255
BspMI ACCTGC 1 cut(s) 508
BspPI GGATC 3 cut(s) 414, 482, 641
BspTI CTTAAG 1 cut(s) 113
BsrI ACTGG 2 cut(s) 180, 445
BssECI CCNNGG 2 cut(s) 64, 364
BssMI GATC 4 cut(s) 406, 487, 646, 735
BssNAI GTATAC 1 cut(s) 523
BssNI GRCGYC 1 cut(s) 707
BssT1I CCWWGG 1 cut(s) 364
Bst1107I GTATAC 1 cut(s) 523
Bst4CI ACNGT 1 cut(s) 65
Bst6I CTCTTC 1 cut(s) 5
BstACI GRCGYC 1 cut(s) 707
BstAFI CTTAAG 1 cut(s) 113
BstBAI YACGTR 1 cut(s) 840
BstC8I GCNNGC 2 cut(s) 28, 48
BstDEI CTNAG 1 cut(s) 246
BstDSI CCRYGG 1 cut(s) 64
BstF5I GGATG 1 cut(s) 594
BstFNI CGCG 1 cut(s) 255
BstKTI GATC 4 cut(s) 409, 490, 649, 738
BstMAI GTCTC 2 cut(s) 90, 698
BstMBI GATC 4 cut(s) 406, 487, 646, 735
BstNSI RCATGY 1 cut(s) 507
BstSFI CTRYAG 1 cut(s) 31
BstUI CGCG 1 cut(s) 255
BstV1I GCAGC 1 cut(s) 589
BstX2I RGATCY 2 cut(s) 406, 646
BstYI RGATCY 2 cut(s) 406, 646
BstZ17I GTATAC 1 cut(s) 523
BsuRI GGCC 1 cut(s) 179
BtgI CCRYGG 1 cut(s) 64
BtgZI GCGATG 1 cut(s) 270
BtsCI GGATG 1 cut(s) 594
BtsIMutI CAGTG 1 cut(s) 187
BveI ACCTGC 1 cut(s) 508
Cac8I GCNNGC 2 cut(s) 28, 48
Cfr13I GGNCC 1 cut(s) 185
Csp6I GTAC 2 cut(s) 4, 36
CviAII CATG 4 cut(s) 504, 545, 668, 727
CviJI RGCY 6 cut(s) 16, 30, 46, 179, 403, 580
CviKI_1 RGCY 6 cut(s) 16, 30, 46, 179, 403, 580
CviQI GTAC 2 cut(s) 4, 36
DdeI CTNAG 1 cut(s) 246
DpnI GATC 4 cut(s) 408, 489, 648, 737
DpnII GATC 4 cut(s) 406, 487, 646, 735
EaeI YGGCCR 1 cut(s) 177
Eam1104I CTCTTC 1 cut(s) 5
EarI CTCTTC 1 cut(s) 5
Eco130I CCWWGG 1 cut(s) 364
Eco32I GATATC 1 cut(s) 823
Eco47I GGWCC 1 cut(s) 185
Eco57I CTGAAG 2 cut(s) 29, 636
EcoRI GAATTC 1 cut(s) 426
EcoRV GATATC 1 cut(s) 823
EcoT14I CCWWGG 1 cut(s) 364
ErhI CCWWGG 1 cut(s) 364
Esp3I CGTCTC 1 cut(s) 698
FaeI CATG 4 cut(s) 507, 548, 671, 730
FatI CATG 4 cut(s) 503, 544, 667, 726
FblI GTMKAC 1 cut(s) 522
Fnu4HI GCNGC 1 cut(s) 578
FokI GGATG 1 cut(s) 601
Fsp4HI GCNGC 1 cut(s) 578
FspBI CTAG 1 cut(s) 582
GluI GCNGC 1 cut(s) 578
HaeIII GGCC 1 cut(s) 179
Hin1I GRCGYC 1 cut(s) 707
Hin1II CATG 4 cut(s) 507, 548, 671, 730
HindIII AAGCTT 1 cut(s) 44
HinfI GANTC 5 cut(s) 161, 626, 662, 680, 833
HphI GGTGA 2 cut(s) 32, 635
Hpy166II GTNNAC 3 cut(s) 185, 450, 523
Hpy188I TCNGA 1 cut(s) 249
Hpy8I GTNNAC 3 cut(s) 185, 450, 523
HpyAV CCTTC 3 cut(s) 49, 112, 414
HpyCH4III ACNGT 1 cut(s) 65
HpyCH4IV ACGT 3 cut(s) 193, 707, 839
HpyCH4V TGCA 5 cut(s) 133, 260, 416, 497, 770
HpyF3I CTNAG 1 cut(s) 246
HpySE526I ACGT 3 cut(s) 193, 707, 839
Hsp92I GRCGYC 1 cut(s) 707
Hsp92II CATG 4 cut(s) 507, 548, 671, 730
Kzo9I GATC 4 cut(s) 406, 487, 646, 735
LmnI GCTCC 1 cut(s) 74
LpnPI CCDG 7 cut(s) 12, 20, 193, 362, 389, 458, 503
Lsp1109I GCAGC 1 cut(s) 589
LweI GCATC 5 cut(s) 13, 120, 247, 313, 757
MaeI CTAG 1 cut(s) 582
MaeII ACGT 3 cut(s) 193, 707, 839
MaeIII GTNAC 1 cut(s) 663
MalI GATC 4 cut(s) 408, 489, 648, 737
MboI GATC 4 cut(s) 406, 487, 646, 735
MboII GAAGA 5 cut(s) 22, 164, 656, 785, 806
MflI RGATCY 2 cut(s) 406, 646
MlsI TGGCCA 1 cut(s) 179
MluCI AATT 5 cut(s) 96, 348, 426, 460, 553
MluNI TGGCCA 1 cut(s) 179
MlyI GAGTC 2 cut(s) 671, 827
MmeI TCCRAC 1 cut(s) 679
MnlI CCTC 7 cut(s) 6, 133, 198, 578, 628, 631, 820
Mox20I TGGCCA 1 cut(s) 179
MscI TGGCCA 1 cut(s) 179
MseI TTAA 3 cut(s) 114, 459, 786
MslI CAYNNNNRTG 4 cut(s) 502, 672, 720, 731
Msp20I TGGCCA 1 cut(s) 179
MspCI CTTAAG 1 cut(s) 113
Mva1269I GAATGC 1 cut(s) 757
MvnI CGCG 1 cut(s) 255
NdeII GATC 4 cut(s) 406, 487, 646, 735
NlaIII CATG 4 cut(s) 507, 548, 671, 730
NmuCI GTSAC 1 cut(s) 663
NspI RCATGY 1 cut(s) 507
PctI GAATGC 1 cut(s) 757
PfeI GAWTC 3 cut(s) 161, 626, 680
PflMI CCANNNNNTGG 1 cut(s) 474
PkrI GCNGC 1 cut(s) 579
PleI GAGTC 2 cut(s) 670, 827
PpsI GAGTC 2 cut(s) 670, 827
Ppu21I YACGTR 1 cut(s) 840
PspPI GGNCC 1 cut(s) 185
PsuI RGATCY 2 cut(s) 406, 646
RsaI GTAC 2 cut(s) 5, 37
RsaNI GTAC 2 cut(s) 4, 36
RseI CAYNNNNRTG 4 cut(s) 502, 672, 720, 731
SaqAI TTAA 3 cut(s) 114, 459, 786
SatI GCNGC 1 cut(s) 578
Sau3AI GATC 4 cut(s) 406, 487, 646, 735
Sau96I GGNCC 1 cut(s) 185
SchI GAGTC 2 cut(s) 671, 827
SfaNI GCATC 5 cut(s) 13, 120, 247, 313, 757
SfcI CTRYAG 1 cut(s) 31
SinI GGWCC 1 cut(s) 185
SmiMI CAYNNNNRTG 4 cut(s) 502, 672, 720, 731
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
Sse9I AATT 5 cut(s) 96, 348, 426, 460, 553
SspMI CTAG 1 cut(s) 582
StyI CCWWGG 1 cut(s) 364
TaaI ACNGT 1 cut(s) 65
TaiI ACGT 3 cut(s) 196, 710, 842
TaqI TCGA 3 cut(s) 321, 678, 825
TasI AATT 5 cut(s) 96, 348, 426, 460, 553
TfiI GAWTC 3 cut(s) 161, 626, 680
Tru1I TTAA 3 cut(s) 114, 459, 786
Tru9I TTAA 3 cut(s) 114, 459, 786
TscAI CASTG 1 cut(s) 187
TseFI GTSAC 1 cut(s) 663
TseI GCWGC 1 cut(s) 577
Tsp45I GTSAC 1 cut(s) 663
TspDTI ATGAA 4 cut(s) 230, 497, 618, 807
TspRI CASTG 1 cut(s) 187
Van91I CCANNNNNTGG 1 cut(s) 474
Vha464I CTTAAG 1 cut(s) 113
VpaK11BI GGWCC 1 cut(s) 185
XapI RAATTY 1 cut(s) 426
XceI RCATGY 1 cut(s) 507
XmiI GTMKAC 1 cut(s) 522
XspI CTAG 1 cut(s) 582
ZraI GACGTC 1 cut(s) 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.