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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 260 |
| AccII | CGCG | 1 cut(s) | 123 |
| AciI | CCGC | 1 cut(s) | 123 |
| AcuI | CTGAAG | 1 cut(s) | 78 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 110 |
| AgsI | TTSAA | 1 cut(s) | 87 |
| AluBI | AGCT | 5 cut(s) | 134, 232, 269, 302, 311 |
| AluI | AGCT | 5 cut(s) | 134, 232, 269, 302, 311 |
| AoxI | GGCC | 1 cut(s) | 137 |
| ApeKI | GCWGC | 3 cut(s) | 229, 266, 308 |
| AspS9I | GGNCC | 2 cut(s) | 98, 137 |
| AsuHPI | GGTGA | 1 cut(s) | 56 |
| AvaII | GGWCC | 1 cut(s) | 98 |
| BbvI | GCAGC | 3 cut(s) | 241, 278, 320 |
| BccI | CCATC | 2 cut(s) | 241, 418 |
| BfaI | CTAG | 2 cut(s) | 345, 445 |
| BfmI | CTRYAG | 1 cut(s) | 264 |
| BglII | AGATCT | 2 cut(s) | 66, 152 |
| BisI | GCNGC | 4 cut(s) | 124, 230, 267, 309 |
| BlpI | GCTNAGC | 2 cut(s) | 270, 312 |
| BlsI | GCNGC | 4 cut(s) | 125, 231, 268, 310 |
| Bme18I | GGWCC | 1 cut(s) | 98 |
| BmgT120I | GGNCC | 2 cut(s) | 98, 137 |
| BmsI | GCATC | 1 cut(s) | 163 |
| Bpu10I | CCTNAGC | 1 cut(s) | 127 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 270, 312 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 110 |
| Bse1I | ACTGG | 1 cut(s) | 46 |
| Bse3DI | GCAATG | 1 cut(s) | 231 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 110 |
| BseMI | GCAATG | 1 cut(s) | 231 |
| BseMII | CTCAG | 3 cut(s) | 173, 284, 326 |
| BseNI | ACTGG | 1 cut(s) | 46 |
| BseRI | GAGGAG | 2 cut(s) | 254, 296 |
| BseXI | GCAGC | 3 cut(s) | 241, 278, 320 |
| BseYI | CCCAGC | 1 cut(s) | 134 |
| BsgI | GTGCAG | 1 cut(s) | 327 |
| Bsh1236I | CGCG | 1 cut(s) | 123 |
| BshFI | GGCC | 1 cut(s) | 139 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 110 |
| BsnI | GGCC | 1 cut(s) | 139 |
| Bsp143I | GATC | 4 cut(s) | 66, 107, 152, 205 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 270, 312 |
| BspACI | CCGC | 1 cut(s) | 123 |
| BspANI | GGCC | 1 cut(s) | 139 |
| BspCNI | CTCAG | 3 cut(s) | 174, 283, 325 |
| BspFNI | CGCG | 1 cut(s) | 123 |
| BspMAI | CTGCAG | 1 cut(s) | 268 |
| BsrDI | GCAATG | 1 cut(s) | 231 |
| BsrI | ACTGG | 1 cut(s) | 46 |
| BssMI | GATC | 4 cut(s) | 66, 107, 152, 205 |
| Bst4CI | ACNGT | 1 cut(s) | 295 |
| Bst6I | CTCTTC | 2 cut(s) | 318, 360 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 173 |
| BstC8I | GCNNGC | 1 cut(s) | 234 |
| BstDEI | CTNAG | 4 cut(s) | 127, 182, 270, 312 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 108 |
| BstFNI | CGCG | 1 cut(s) | 123 |
| BstKTI | GATC | 4 cut(s) | 69, 110, 155, 208 |
| BstMBI | GATC | 4 cut(s) | 66, 107, 152, 205 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 120, 173, 209, 308, 341, 383 |
| BstSFI | CTRYAG | 1 cut(s) | 264 |
| BstUI | CGCG | 1 cut(s) | 123 |
| BstV1I | GCAGC | 3 cut(s) | 241, 278, 320 |
| BstX2I | RGATCY | 2 cut(s) | 66, 152 |
| BstYI | RGATCY | 2 cut(s) | 66, 152 |
| BsuRI | GGCC | 1 cut(s) | 139 |
| Cac8I | GCNNGC | 1 cut(s) | 234 |
| Cfr13I | GGNCC | 2 cut(s) | 98, 137 |
| DdeI | CTNAG | 4 cut(s) | 127, 182, 270, 312 |
| DpnI | GATC | 4 cut(s) | 68, 109, 154, 207 |
| DpnII | GATC | 4 cut(s) | 66, 107, 152, 205 |
| Eam1104I | CTCTTC | 2 cut(s) | 318, 360 |
| EarI | CTCTTC | 2 cut(s) | 318, 360 |
| Eco47I | GGWCC | 1 cut(s) | 98 |
| Eco57I | CTGAAG | 1 cut(s) | 78 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 108 |
| FaiI | YATR | 3 cut(s) | 72, 117, 119 |
| FblI | GTMKAC | 1 cut(s) | 260 |
| Fnu4HI | GCNGC | 4 cut(s) | 124, 230, 267, 309 |
| Fsp4HI | GCNGC | 4 cut(s) | 124, 230, 267, 309 |
| FspBI | CTAG | 2 cut(s) | 345, 445 |
| GluI | GCNGC | 4 cut(s) | 124, 230, 267, 309 |
| GsaI | CCCAGC | 1 cut(s) | 138 |
| HaeIII | GGCC | 1 cut(s) | 139 |
| HinfI | GANTC | 1 cut(s) | 7 |
| HphI | GGTGA | 1 cut(s) | 56 |
| Hpy166II | GTNNAC | 2 cut(s) | 101, 261 |
| Hpy188I | TCNGA | 1 cut(s) | 399 |
| Hpy8I | GTNNAC | 2 cut(s) | 101, 261 |
| HpyCH4III | ACNGT | 1 cut(s) | 295 |
| HpyCH4V | TGCA | 4 cut(s) | 229, 236, 266, 308 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 120, 173, 209, 308, 341, 383 |
| HpyF3I | CTNAG | 4 cut(s) | 127, 182, 270, 312 |
| Kzo9I | GATC | 4 cut(s) | 66, 107, 152, 205 |
| LmnI | GCTCC | 1 cut(s) | 131 |
| LpnPI | CCDG | 4 cut(s) | 27, 96, 120, 288 |
| Lsp1109I | GCAGC | 3 cut(s) | 241, 278, 320 |
| LweI | GCATC | 1 cut(s) | 163 |
| MaeI | CTAG | 2 cut(s) | 345, 445 |
| MalI | GATC | 4 cut(s) | 68, 109, 154, 207 |
| MboI | GATC | 4 cut(s) | 66, 107, 152, 205 |
| MboII | GAAGA | 5 cut(s) | 16, 47, 71, 335, 377 |
| MflI | RGATCY | 2 cut(s) | 66, 152 |
| MluCI | AATT | 3 cut(s) | 90, 419, 431 |
| MnlI | CCTC | 7 cut(s) | 114, 177, 181, 232, 274, 325, 367 |
| MseI | TTAA | 2 cut(s) | 287, 417 |
| MvnI | CGCG | 1 cut(s) | 123 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 120, 173, 209, 308, 341, 383 |
| NdeII | GATC | 4 cut(s) | 66, 107, 152, 205 |
| PfeI | GAWTC | 1 cut(s) | 7 |
| PkrI | GCNGC | 4 cut(s) | 125, 231, 268, 310 |
| PspFI | CCCAGC | 1 cut(s) | 134 |
| PspPI | GGNCC | 2 cut(s) | 98, 137 |
| PstI | CTGCAG | 1 cut(s) | 268 |
| PsuI | RGATCY | 2 cut(s) | 66, 152 |
| SaqAI | TTAA | 2 cut(s) | 287, 417 |
| SatI | GCNGC | 4 cut(s) | 124, 230, 267, 309 |
| Sau3AI | GATC | 4 cut(s) | 66, 107, 152, 205 |
| Sau96I | GGNCC | 2 cut(s) | 98, 137 |
| SetI | ASST | 6 cut(s) | 106, 136, 234, 271, 304, 313 |
| SfaNI | GCATC | 1 cut(s) | 163 |
| SfcI | CTRYAG | 1 cut(s) | 264 |
| SinI | GGWCC | 1 cut(s) | 98 |
| Sse9I | AATT | 3 cut(s) | 90, 419, 431 |
| SsiI | CCGC | 1 cut(s) | 123 |
| SspMI | CTAG | 2 cut(s) | 345, 445 |
| TaaI | ACNGT | 1 cut(s) | 295 |
| TaqI | TCGA | 1 cut(s) | 106 |
| TaqII | GACCGA | 1 cut(s) | 416 |
| TasI | AATT | 3 cut(s) | 90, 419, 431 |
| TauI | GCSGC | 1 cut(s) | 126 |
| TfiI | GAWTC | 1 cut(s) | 7 |
| Tru1I | TTAA | 2 cut(s) | 287, 417 |
| Tru9I | TTAA | 2 cut(s) | 287, 417 |
| TseI | GCWGC | 3 cut(s) | 229, 266, 308 |
| TspDTI | ATGAA | 5 cut(s) | 17, 44, 48, 336, 378 |
| VpaK11BI | GGWCC | 1 cut(s) | 98 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 108 |
| XmiI | GTMKAC | 1 cut(s) | 260 |
| XspI | CTAG | 2 cut(s) | 345, 445 |