Rh2BG413400

Plant transposase (Ptta/En/Spm family)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000178
Physical Location & Seq
Reverse (-)
58340492 .. 58348975
8484 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG413400.1

Sequence Viewer

Length: 1188 bp
ATGGCCAAGAGATCACAAGGTCCTAAATTTTTGCATCAATTGCGAACCTCAGCACTTCCAGCAAACCACTTGCGAACATCTGTTGAAAAGGTTTCTAGGCATGTAAAGCCTTTAGATAAACCTACAACACCTGCATGTCCATTGCGATCATCAACTGAAAAGGTTTCTAAGGTTATAAAGCCTTTAGTTAAACCTACAACACTTTCAAGCCCATTACGGTTATCTCCTAGAAAGCATTCAAAGACACAACCTTCCACAAATCGATCAATACATCCTCGTCGACCTGCTATCCAGTCATCAAGAACTCCATCTCCTCCTTCCCCATATGTTATGAGATCATCTCCGCTTTCCCCTTCACCTCCACATATGCGCACAGTCTTATCAACCTCTAATCAGTACACATCAACTCCAAGTGCACACGAAGAGATAGCTGAATCTTCTCAAGTTGCTCATCCTCCTACCTTAGTAGAGAACATTGGTGCCACCCAGAAGAAACGACGTGGTGAGACTCGAGGTCTTGGGACAGCCAAGAAGAAACGACCTAGTAATCAAATAGAGATTGATATTCCACAGCATGTAAAACGAGCCGTAGGAGAGAATTGCCAGTCTTACATCACAGAGATAGGCTGCATTGTTAGGCAAAATGCTCCATTACAAGTTAAGCATTGGAGTGGAATTAATAGGGATGATGTTGCTACGATGGTTCGTCTTGTCCGTGAGAAATTCAAATTGGGGAATGAACCACACGTGAATGAGGCTATTGAGGCAGACATGAAAAGAAGATATAGCACTTGGCGATACAATTTGCATAAGATGTTTTTGCAATATGAATCAGCAGAGGAGGCACTTGAGAATAGACCTGAAAATGTGGGAGAAGATGACTGGGATTTTCTCATCAATTGGTGGCACGATGATGAGTGGCTGGAATTGAGCAGAAAAAATAAGAAAAACAGAGATAAGCTAACAATAACTCATTGTGCTGGGACAAAAGCATTTAGTCGCATTAGATATGAAAATCAAAATTCTGAGACCGGAGAGGAACCTAGCCGTATTGACATGTTCAAGCTGACAAGATTTAGCGAAAACAAGAAAACATGGGTCGGAGATGTCGCTGAAAATGCTTATGGTGAGATGACAAAACTAAGAAATCCTGAGCAAACAGATGAAGGGTCAGAAAAAGTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transposase_24

Protein Analysis

395

Amino Acids

45.12

Weight (kDa)

9.71

Isoelectric Point (pI)

57.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_24 PF03004 292 - 392 8.2e-15 Plant transposase (Ptta/En/Spm family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G40087 AT3G30200
fragaria_vesca FvH4_1g23271 FvH4_3g22812 FvH4_3g22813 FvH4_3g31042 FvH4_3g31043 FvH4_7g03653
malus_domestica MD05G1321200.v1.1 MD09G1027200.v1.1 MD15G1434900.v1.1 MD15G1435000.v1.1 MD15G1435100.v1.1
prunus_persica Prupe.1G578800_v2.0.a1 Prupe.1G578800_v2.0.a1 Prupe.1G578800_v2.0.a1 Prupe.2G062700_v2.0.a1 Prupe.5G033400_v2.0.a1 Prupe.7G022900_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1
pyrus_communis pycom03g07600 pycom03g11360 pycom05g02870 pycom111g02130 pycom15g38370 pycom15g38380
rosa_chinensis RchiOBHm_Chr1g0346371 RchiOBHm_Chr4g0402051 RchiOBHm_Chr4g0414991 RchiOBHm_Chr5g0051011 RchiOBHm_Chr5g0051181 RchiOBHm_Chr6g0273071 RchiOBHm_Chr7g0218301 RchiOBHm_Chr7g0241081
rosa_laevigata RLG00000007709 RLG00000008953 RLG00000012400 RLG00000012401 RLG00000013805 RLG00000014199 RLG00000014726 RLG00000017792 RLG00000017793 RLG00000018860 RLG00000020749 RLG00000030708 RLG00000033507
rosa_multiflora Rmu_co8373761.1_g000001 Rmu_sc0000028.1_g000023 Rmu_sc0000235.1_g000040 Rmu_sc0000293.1_g000017 Rmu_sc0000293.1_g000019 Rmu_sc0000651.1_g000012 Rmu_sc0000870.1_g000051 Rmu_sc0001167.1_g000031 Rmu_sc0001373.1_g000039 Rmu_sc0001861.1_g000055 Rmu_sc0001891.1_g000038 Rmu_sc0001909.1_g000015 Rmu_sc0002500.1_g000002 Rmu_sc0002868.1_g000036 Rmu_sc0002868.1_g000038 Rmu_sc0002868.1_g000039 Rmu_sc0003379.1_g000001 Rmu_sc0003778.1_g000005 Rmu_sc0003778.1_g000006 Rmu_sc0010475.1_g000011 Rmu_sc0015271.1_g000002 Rmu_sc0015271.1_g000003 Rmu_sc0017962.1_g000001 Rmu_sc0017962.1_g000003 Rmu_sc0022129.1_g000004 Rmu_sc0025694.1_g000003 Rmu_sc0025694.1_g000004
rosa_roxburghii Rroxscaffold_1G00031640 Rroxscaffold_1G00047620 Rroxscaffold_1G00047630 Rroxscaffold_1G00050560 Rroxscaffold_1G00050570 Rroxscaffold_1G00054800 Rroxscaffold_1G00055890 Rroxscaffold_2G00108230 Rroxscaffold_3G00240860 Rroxscaffold_4G00299670 Rroxscaffold_4G00299680 Rroxscaffold_4G00299690 Rroxscaffold_4G00306320 Rroxscaffold_4G00306340 Rroxscaffold_5G00341060 Rroxscaffold_5G00342000 Rroxscaffold_5G00355660 Rroxscaffold_5G00357170 Rroxscaffold_6G00399490 Rroxscaffold_6G00408400 Rroxscaffold_7G00189260 Rroxscaffold_7G00199620 Rroxscaffold_7G00215470
rosa_rugosa Rorug02G0117600 Rorug02G0117700 Rorug04G0116400 Rorug06G0103200
rosa_samantha Rh1AG062800 Rh1AG073700 Rh1CG091700 Rh1DG077600 Rh1DG077700 Rh1DG077800 Rh1DG117400 Rh1DG146300 Rh2BG193300 Rh2BG193400 Rh2BG265300 Rh2BG413400 Rh3CG365500 Rh4AG315400 Rh4BG323100 Rh4BG323200 Rh4CG071400 Rh4CG142500 Rh4CG203300 Rh4CG338300 Rh4DG079300 Rh4DG079400 Rh4DG217000 Rh4DG217100 Rh4DG217200 Rh4DG217300 Rh5AG128300 Rh5AG239900 Rh5AG465900 Rh5BG348600 Rh5BG387900 Rh5BG388000 Rh5CG181800 Rh5CG181900 Rh5CG271000 Rh5CG271100 Rh5DG127100 Rh5DG138400 Rh5DG168200 Rh5DG168300 Rh5DG361500 Rh5DG460400 Rh6AG045400 Rh6AG058600 Rh6BG275000 Rh6CG089700 Rh6DG182300 Rh6DG182400 Rh6DG207300 Rh6DG207400 Rh7BG374000 Rh7BG390600 Rh7BG390700 Rh7BG390800
rosa_wichuraiana Rw0G018690 Rw1G007320 Rw4G008510 Rw5G011510 Rw5G021830 Rw6G024150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 176
AarI CACCTGC 1 cut(s) 139
Acc16I TGCGCA 1 cut(s) 371
Acc36I ACCTGC 2 cut(s) 139, 292
AccB1I GGYRCC 1 cut(s) 479
AccI GTMKAC 1 cut(s) 280
AciI CCGC 1 cut(s) 344
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 26, 722, 1021
AcvI CACGTG 1 cut(s) 748
AfaI GTAC 1 cut(s) 398
AflIII ACRYGT 2 cut(s) 745, 1056
AgsI TTSAA 5 cut(s) 86, 207, 240, 727, 1063
AhdI GACNNNNNGTC 1 cut(s) 513
AjiI CACGTC 1 cut(s) 500
AjuI GAANNNNNNNTTGG 2 cut(s) 713, 745
AloI GAACNNNNNNTCC 2 cut(s) 295, 327
AluBI AGCT 3 cut(s) 431, 961, 1066
AluI AGCT 3 cut(s) 431, 961, 1066
Alw21I GWGCWC 1 cut(s) 418
Alw26I GTCTC 2 cut(s) 500, 1022
Alw44I GTGCAC 1 cut(s) 414
Ama87I CYCGRG 1 cut(s) 510
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 414
ApeKI GCWGC 1 cut(s) 627
ApoI RAATTY 3 cut(s) 26, 722, 1021
AseI ATTAAT 1 cut(s) 678
Asp700I GAANNNNTTC 1 cut(s) 235
AspLEI GCGC 1 cut(s) 372
AspS9I GGNCC 1 cut(s) 20
AsuHPI GGTGA 3 cut(s) 348, 515, 1139
AvaI CYCGRG 1 cut(s) 510
AvaII GGWCC 1 cut(s) 20
BaeGI GKGCMC 1 cut(s) 418
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 479
BbrPI CACGTG 1 cut(s) 748
Bbv12I GWGCWC 1 cut(s) 418
BbvCI CCTCAGC 1 cut(s) 49
BbvI GCAGC 1 cut(s) 614
BccI CCATC 2 cut(s) 316, 694
BceAI ACGGC 2 cut(s) 572, 1032
BcoDI GTCTC 2 cut(s) 500, 1022
BfaI CTAG 4 cut(s) 96, 228, 543, 1044
BfuAI ACCTGC 2 cut(s) 139, 292
BisI GCNGC 1 cut(s) 628
BlsI GCNGC 1 cut(s) 629
Bme18I GGWCC 1 cut(s) 20
BmeRI GACNNNNNGTC 1 cut(s) 513
BmeT110I CYCGRG 1 cut(s) 510
BmgBI CACGTC 1 cut(s) 500
BmgT120I GGNCC 1 cut(s) 20
BmiI GGNNCC 2 cut(s) 481, 1041
BmrI ACTGGG 1 cut(s) 892
BmsI GCATC 1 cut(s) 43
BmuI ACTGGG 1 cut(s) 892
BplI GAGNNNNNCTC 2 cut(s) 325, 357
Bpu10I CCTNAGC 2 cut(s) 49, 1152
BpuEI CTTGAG 2 cut(s) 426, 869
Bsa29I ATCGAT 1 cut(s) 262
BsaAI YACGTR 1 cut(s) 748
BsaI GGTCTC 1 cut(s) 1022
BsaWI WCCGGW 1 cut(s) 1031
BsaXI ACNNNNNCTCC 4 cut(s) 295, 325, 391, 421
Bse1I ACTGG 3 cut(s) 292, 604, 887
Bse3DI GCAATG 1 cut(s) 140
BseCI ATCGAT 1 cut(s) 262
BseGI GGATG 3 cut(s) 271, 451, 691
BseMI GCAATG 1 cut(s) 140
BseMII CTCAG 3 cut(s) 63, 1017, 1143
BseNI ACTGG 3 cut(s) 292, 604, 887
BseRI GAGGAG 2 cut(s) 303, 854
BseSI GKGCMC 1 cut(s) 418
BseXI GCAGC 1 cut(s) 614
BseYI CCCAGC 1 cut(s) 980
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 479
BshVI ATCGAT 1 cut(s) 262
BsiHKAI GWGCWC 1 cut(s) 418
BsiHKCI CYCGRG 1 cut(s) 510
BsiSI CCGG 1 cut(s) 1032
BslFI GGGAC 2 cut(s) 535, 997
BsmAI GTCTC 2 cut(s) 500, 1022
BsmFI GGGAC 2 cut(s) 535, 997
BsmI GAATGC 1 cut(s) 235
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 1022
BsoBI CYCGRG 1 cut(s) 510
Bsp1286I GDGCHC 1 cut(s) 418
Bsp143I GATC 4 cut(s) 11, 146, 263, 335
BspACI CCGC 1 cut(s) 344
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 3 cut(s) 62, 1018, 1144
BspDI ATCGAT 1 cut(s) 262
BspLI GGNNCC 2 cut(s) 481, 1041
BspMI ACCTGC 2 cut(s) 139, 292
BspT107I GGYRCC 1 cut(s) 479
BspTNI GGTCTC 1 cut(s) 1022
BsrDI GCAATG 1 cut(s) 140
BsrI ACTGG 3 cut(s) 292, 604, 887
BssMI GATC 4 cut(s) 11, 146, 263, 335
Bst4CI ACNGT 2 cut(s) 219, 376
Bst6I CTCTTC 1 cut(s) 417
BstAPI GCANNNNNTGC 1 cut(s) 40
BstBAI YACGTR 1 cut(s) 748
BstDEI CTNAG 6 cut(s) 49, 168, 463, 1026, 1142, 1152
BstF5I GGATG 3 cut(s) 271, 451, 691
BstHHI GCGC 1 cut(s) 372
BstKTI GATC 4 cut(s) 14, 149, 266, 338
BstMAI GTCTC 2 cut(s) 500, 1022
BstMBI GATC 4 cut(s) 11, 146, 263, 335
BstMWI GCNNNNNNNGC 6 cut(s) 40, 59, 106, 764, 842, 1118
BstNSI RCATGY 4 cut(s) 104, 138, 578, 1060
BstSLI GKGCMC 1 cut(s) 418
BstV1I GCAGC 1 cut(s) 614
Bsu15I ATCGAT 1 cut(s) 262
BsuRI GGCC 1 cut(s) 5
BsuTUI ATCGAT 1 cut(s) 262
BtrI CACGTC 1 cut(s) 500
BtsCI GGATG 3 cut(s) 271, 451, 691
BveI ACCTGC 2 cut(s) 139, 292
CfoI GCGC 1 cut(s) 372
Cfr13I GGNCC 1 cut(s) 20
ClaI ATCGAT 1 cut(s) 262
Csp6I GTAC 1 cut(s) 397
CviAII CATG 6 cut(s) 101, 135, 575, 772, 1057, 1095
CviQI GTAC 1 cut(s) 397
DdeI CTNAG 6 cut(s) 49, 168, 463, 1026, 1142, 1152
DpnI GATC 4 cut(s) 13, 148, 265, 337
DpnII GATC 4 cut(s) 11, 146, 263, 335
DriI GACNNNNNGTC 1 cut(s) 513
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 417
Eam1105I GACNNNNNGTC 1 cut(s) 513
EarI CTCTTC 1 cut(s) 417
Eco31I GGTCTC 1 cut(s) 1022
Eco47I GGWCC 1 cut(s) 20
Eco72I CACGTG 1 cut(s) 748
Eco88I CYCGRG 1 cut(s) 510
EcoO109I RGGNCCY 1 cut(s) 20
FaeI CATG 6 cut(s) 104, 138, 578, 775, 1060, 1098
FaqI GGGAC 2 cut(s) 535, 997
FatI CATG 6 cut(s) 100, 134, 574, 771, 1056, 1094
FauNDI CATATG 2 cut(s) 325, 366
FblI GTMKAC 1 cut(s) 280
Fnu4HI GCNGC 1 cut(s) 628
FokI GGATG 3 cut(s) 258, 438, 698
Fsp4HI GCNGC 1 cut(s) 628
FspAI RTGCGCAY 1 cut(s) 371
FspBI CTAG 4 cut(s) 96, 228, 543, 1044
FspI TGCGCA 1 cut(s) 371
GlaI GCGC 1 cut(s) 371
GluI GCNGC 1 cut(s) 628
GsaI CCCAGC 1 cut(s) 984
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 1032
HhaI GCGC 1 cut(s) 372
Hin1II CATG 6 cut(s) 104, 138, 578, 775, 1060, 1098
Hin6I GCGC 1 cut(s) 370
HinP1I GCGC 1 cut(s) 370
HincII GTYRAC 1 cut(s) 281
HindII GTYRAC 1 cut(s) 281
HinfI GANTC 3 cut(s) 434, 508, 830
HpaII CCGG 1 cut(s) 1032
HphI GGTGA 3 cut(s) 348, 515, 1139
Hpy166II GTNNAC 3 cut(s) 281, 399, 416
Hpy188I TCNGA 3 cut(s) 1027, 1103, 1174
Hpy188III TCNNGA 2 cut(s) 300, 1151
Hpy8I GTNNAC 3 cut(s) 281, 399, 416
Hpy99I CGWCG 2 cut(s) 282, 501
HpyAV CCTTC 4 cut(s) 261, 327, 363, 1160
HpyCH4III ACNGT 2 cut(s) 219, 376
HpyCH4IV ACGT 2 cut(s) 499, 747
HpyCH4V TGCA 6 cut(s) 34, 134, 416, 630, 808, 823
HpyF10VI GCNNNNNNNGC 6 cut(s) 40, 59, 106, 764, 842, 1118
HpyF3I CTNAG 6 cut(s) 49, 168, 463, 1026, 1142, 1152
HpySE526I ACGT 2 cut(s) 499, 747
Hsp92II CATG 6 cut(s) 104, 138, 578, 775, 1060, 1098
HspAI GCGC 1 cut(s) 370
Kzo9I GATC 4 cut(s) 11, 146, 263, 335
LmnI GCTCC 1 cut(s) 652
Lsp1109I GCAGC 1 cut(s) 614
LweI GCATC 1 cut(s) 43
MaeI CTAG 4 cut(s) 96, 228, 543, 1044
MaeII ACGT 2 cut(s) 499, 747
MalI GATC 4 cut(s) 13, 148, 265, 337
MboI GATC 4 cut(s) 11, 146, 263, 335
MboII GAAGA 6 cut(s) 429, 434, 502, 544, 792, 887
MfeI CAATTG 2 cut(s) 38, 898
MhlI GDGCHC 1 cut(s) 418
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 502
MmeI TCCRAC 1 cut(s) 1081
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 235
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 189, 660, 678
MslI CAYNNNNRTG 4 cut(s) 133, 669, 750, 912
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 1032
MunI CAATTG 2 cut(s) 38, 898
Mva1269I GAATGC 1 cut(s) 235
MwoI GCNNNNNNNGC 6 cut(s) 40, 59, 106, 764, 842, 1118
NdeI CATATG 2 cut(s) 325, 366
NdeII GATC 4 cut(s) 11, 146, 263, 335
NlaIII CATG 6 cut(s) 104, 138, 578, 775, 1060, 1098
NlaIV GGNNCC 2 cut(s) 481, 1041
NsbI TGCGCA 1 cut(s) 371
NspI RCATGY 4 cut(s) 104, 138, 578, 1060
PaeR7I CTCGAG 1 cut(s) 510
PaqCI CACCTGC 1 cut(s) 139
PciI ACATGT 1 cut(s) 1056
PcsI WCGNNNNNNNCGW 1 cut(s) 712
PctI GAATGC 1 cut(s) 235
PdmI GAANNNNTTC 1 cut(s) 235
PfeI GAWTC 2 cut(s) 434, 830
PkrI GCNGC 1 cut(s) 629
PleI GAGTC 1 cut(s) 502
PmaCI CACGTG 1 cut(s) 748
PmlI CACGTG 1 cut(s) 748
PpsI GAGTC 1 cut(s) 502
Ppu21I YACGTR 1 cut(s) 748
PpuMI RGGWCCY 1 cut(s) 20
PscI ACATGT 1 cut(s) 1056
PshBI ATTAAT 1 cut(s) 678
PsiI TTATAA 1 cut(s) 176
Psp5II RGGWCCY 1 cut(s) 20
PspCI CACGTG 1 cut(s) 748
PspFI CCCAGC 1 cut(s) 980
PspN4I GGNNCC 2 cut(s) 481, 1041
PspPI GGNCC 1 cut(s) 20
PspPPI RGGWCCY 1 cut(s) 20
PspXI VCTCGAGB 1 cut(s) 510
RsaI GTAC 1 cut(s) 398
RsaNI GTAC 1 cut(s) 397
RseI CAYNNNNRTG 4 cut(s) 133, 669, 750, 912
SalI GTCGAC 1 cut(s) 279
SaqAI TTAA 3 cut(s) 189, 660, 678
SatI GCNGC 1 cut(s) 628
Sau3AI GATC 4 cut(s) 11, 146, 263, 335
Sau96I GGNCC 1 cut(s) 20
SchI GAGTC 1 cut(s) 502
SduI GDGCHC 1 cut(s) 418
SfaNI GCATC 1 cut(s) 43
Sfr274I CTCGAG 1 cut(s) 510
SinI GGWCC 1 cut(s) 20
SlaI CTCGAG 1 cut(s) 510
SmiMI CAYNNNNRTG 4 cut(s) 133, 669, 750, 912
SmlI CTYRAG 3 cut(s) 441, 510, 848
SmoI CTYRAG 3 cut(s) 441, 510, 848
SsiI CCGC 1 cut(s) 344
SspMI CTAG 4 cut(s) 96, 228, 543, 1044
TaaI ACNGT 2 cut(s) 219, 376
TaiI ACGT 2 cut(s) 502, 750
TaqI TCGA 3 cut(s) 262, 280, 511
TatI WGTACW 1 cut(s) 396
TfiI GAWTC 2 cut(s) 434, 830
Tru1I TTAA 3 cut(s) 189, 660, 678
Tru9I TTAA 3 cut(s) 189, 660, 678
TseI GCWGC 1 cut(s) 627
TspDTI ATGAA 5 cut(s) 753, 788, 843, 1026, 1179
TspGWI ACGGA 1 cut(s) 704
VneI GTGCAC 1 cut(s) 414
VpaK11BI GGWCC 1 cut(s) 20
VspI ATTAAT 1 cut(s) 678
XapI RAATTY 3 cut(s) 26, 722, 1021
XceI RCATGY 4 cut(s) 104, 138, 578, 1060
XhoI CTCGAG 1 cut(s) 510
XmiI GTMKAC 1 cut(s) 280
XmnI GAANNNNTTC 1 cut(s) 235
XspI CTAG 4 cut(s) 96, 228, 543, 1044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.