Rh4DG217200

Domain of unknown function (DUF4216)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000178
Physical Location & Seq
Reverse (-)
41728782 .. 41729495
714 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG217200.1

Sequence Viewer

Length: 714 bp
ATGCAAGTTGAGGGTTCAACAGAAGATGTTGAATCCTTACTATACTTGGCCAGTGGACCTCAACGTGAAGTGGCACGATATAGTGGGTGTATCGTCAATGGCATAAGGTTTCATACTCAGAAACGCGATGCAAATAAAAAAACTCAAAACTATGGAGTGGTAGTCAAAGGAGAGCATCGTGGTAAAAGTATAGACTTTTATGGTGTTTTGAAAGATATAATTGTGTTATCATACCTTGGAAATAATCAGGTGGTCGTTTTCAAATGTGATTGGCTGGATCTCAATGCAAGAAGGGGAATTCAAGTTGATGAGAACCAGTTTACGAGTGTTAATTTTACCAAAAAATGGTATATGAATGATCCATTTGCATTGGCATGTCAAACACAGCAGGTATACTATTTGAAAGATACAAAGCATGGTAGTAATTGGCGTGTTGTAGAAAGGTCGCAGCCTAGAGGGATGTATGATTTTACAGAAAAAGAAACTGAAGTGGGCGATTCATTAGAGGAGGTAGAAGATCCATATCAGCAAGAGTCACATGGGTATGTCGATTCAGTTGAAGTTGATGTTGGAGAGACGTCATTACATAGAGATGACATGGATATGATCGTTGTTGATTTGAATACTAAAGATAGTGATGCAGAAGATGGCGACTTTAATGATGAAGATAATGAGTTGATGTCTGATGATGATATCGAGGACGACTCTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218

Protein Analysis

237

Amino Acids

27.16

Weight (kDa)

4.38

Isoelectric Point (pI)

27.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4216 PF13952 76 - 146 4.8e-24 Domain of unknown function (DUF4216)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G40087 AT3G30200
fragaria_vesca FvH4_1g23271 FvH4_3g22812 FvH4_3g22813 FvH4_3g31042 FvH4_3g31043 FvH4_7g03653
malus_domestica MD05G1321200.v1.1 MD09G1027200.v1.1 MD15G1434900.v1.1 MD15G1435000.v1.1 MD15G1435100.v1.1
prunus_persica Prupe.1G578800_v2.0.a1 Prupe.1G578800_v2.0.a1 Prupe.1G578800_v2.0.a1 Prupe.2G062700_v2.0.a1 Prupe.5G033400_v2.0.a1 Prupe.7G022900_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1
pyrus_communis pycom03g07600 pycom03g11360 pycom05g02870 pycom111g02130 pycom15g38370 pycom15g38380
rosa_chinensis RchiOBHm_Chr1g0346371 RchiOBHm_Chr4g0402051 RchiOBHm_Chr4g0414991 RchiOBHm_Chr5g0051011 RchiOBHm_Chr5g0051181 RchiOBHm_Chr6g0273071 RchiOBHm_Chr7g0218301 RchiOBHm_Chr7g0241081
rosa_laevigata RLG00000007709 RLG00000008953 RLG00000012400 RLG00000012401 RLG00000013805 RLG00000014199 RLG00000014726 RLG00000017792 RLG00000017793 RLG00000018860 RLG00000020749 RLG00000030708 RLG00000033507
rosa_multiflora Rmu_co8373761.1_g000001 Rmu_sc0000028.1_g000023 Rmu_sc0000235.1_g000040 Rmu_sc0000293.1_g000017 Rmu_sc0000293.1_g000019 Rmu_sc0000651.1_g000012 Rmu_sc0000870.1_g000051 Rmu_sc0001167.1_g000031 Rmu_sc0001373.1_g000039 Rmu_sc0001861.1_g000055 Rmu_sc0001891.1_g000038 Rmu_sc0001909.1_g000015 Rmu_sc0002500.1_g000002 Rmu_sc0002868.1_g000036 Rmu_sc0002868.1_g000038 Rmu_sc0002868.1_g000039 Rmu_sc0003379.1_g000001 Rmu_sc0003778.1_g000005 Rmu_sc0003778.1_g000006 Rmu_sc0010475.1_g000011 Rmu_sc0015271.1_g000002 Rmu_sc0015271.1_g000003 Rmu_sc0017962.1_g000001 Rmu_sc0017962.1_g000003 Rmu_sc0022129.1_g000004 Rmu_sc0025694.1_g000003 Rmu_sc0025694.1_g000004
rosa_roxburghii Rroxscaffold_1G00031640 Rroxscaffold_1G00047620 Rroxscaffold_1G00047630 Rroxscaffold_1G00050560 Rroxscaffold_1G00050570 Rroxscaffold_1G00054800 Rroxscaffold_1G00055890 Rroxscaffold_2G00108230 Rroxscaffold_3G00240860 Rroxscaffold_4G00299670 Rroxscaffold_4G00299680 Rroxscaffold_4G00299690 Rroxscaffold_4G00306320 Rroxscaffold_4G00306340 Rroxscaffold_5G00341060 Rroxscaffold_5G00342000 Rroxscaffold_5G00355660 Rroxscaffold_5G00357170 Rroxscaffold_6G00399490 Rroxscaffold_6G00408400 Rroxscaffold_7G00189260 Rroxscaffold_7G00199620 Rroxscaffold_7G00215470
rosa_rugosa Rorug02G0117600 Rorug02G0117700 Rorug04G0116400 Rorug06G0103200
rosa_samantha Rh1AG062800 Rh1AG073700 Rh1CG091700 Rh1DG077600 Rh1DG077700 Rh1DG077800 Rh1DG117400 Rh1DG146300 Rh2BG193300 Rh2BG193400 Rh2BG265300 Rh2BG413400 Rh3CG365500 Rh4AG315400 Rh4BG323100 Rh4BG323200 Rh4CG071400 Rh4CG142500 Rh4CG203300 Rh4CG338300 Rh4DG079300 Rh4DG079400 Rh4DG217000 Rh4DG217100 Rh4DG217200 Rh4DG217300 Rh5AG128300 Rh5AG239900 Rh5AG465900 Rh5BG348600 Rh5BG387900 Rh5BG388000 Rh5CG181800 Rh5CG181900 Rh5CG271000 Rh5CG271100 Rh5DG127100 Rh5DG138400 Rh5DG168200 Rh5DG168300 Rh5DG361500 Rh5DG460400 Rh6AG045400 Rh6AG058600 Rh6BG275000 Rh6CG089700 Rh6DG182300 Rh6DG182400 Rh6DG207300 Rh6DG207400 Rh7BG374000 Rh7BG390600 Rh7BG390700 Rh7BG390800
rosa_wichuraiana Rw0G018690 Rw1G007320 Rw4G008510 Rw5G011510 Rw5G021830 Rw6G024150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 581
Acc36I ACCTGC 1 cut(s) 379
AccB7I CCANNNNNTGG 1 cut(s) 345
AccI GTMKAC 1 cut(s) 393
AccII CGCG 1 cut(s) 126
AclWI GGATC 3 cut(s) 285, 353, 512
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 1 cut(s) 297
AcuI CTGAAG 1 cut(s) 507
AcyI GRCGYC 1 cut(s) 578
AfiI CCNNNNNNNGG 1 cut(s) 345
AgsI TTSAA 8 cut(s) 18, 32, 211, 262, 302, 403, 560, 622
AjuI GAANNNNNNNTTGG 2 cut(s) 552, 584
Alw26I GTCTC 1 cut(s) 569
AlwI GGATC 3 cut(s) 285, 353, 512
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 1 cut(s) 448
ApoI RAATTY 1 cut(s) 297
AspS9I GGNCC 1 cut(s) 56
AvaII GGWCC 1 cut(s) 56
BalI TGGCCA 1 cut(s) 50
BbvI GCAGC 1 cut(s) 460
BccI CCATC 1 cut(s) 641
BcoDI GTCTC 1 cut(s) 569
BfaI CTAG 1 cut(s) 453
BfuAI ACCTGC 1 cut(s) 379
BisI GCNGC 1 cut(s) 449
BlsI GCNGC 1 cut(s) 450
Bme18I GGWCC 1 cut(s) 56
BmgT120I GGNCC 1 cut(s) 56
BmsI GCATC 3 cut(s) 118, 184, 628
BplI GAGNNNNNCTC 1 cut(s) 689
BsaBI GATNNNNATC 1 cut(s) 522
BsaHI GRCGYC 1 cut(s) 578
BsaJI CCNNGG 1 cut(s) 235
BsaXI ACNNNNNCTCC 2 cut(s) 147, 177
Bsc4I CCNNNNNNNGG 1 cut(s) 345
Bse1I ACTGG 2 cut(s) 51, 316
Bse8I GATNNNNATC 1 cut(s) 522
BseDI CCNNGG 1 cut(s) 235
BseGI GGATG 1 cut(s) 465
BseJI GATNNNNATC 1 cut(s) 522
BseLI CCNNNNNNNGG 1 cut(s) 345
BseMII CTCAG 1 cut(s) 131
BseNI ACTGG 2 cut(s) 51, 316
BseRI GAGGAG 1 cut(s) 521
BseXI GCAGC 1 cut(s) 460
Bsh1236I CGCG 1 cut(s) 126
BshFI GGCC 1 cut(s) 50
BslI CCNNNNNNNGG 1 cut(s) 345
BsmAI GTCTC 1 cut(s) 569
BsmBI CGTCTC 1 cut(s) 569
BsnI GGCC 1 cut(s) 50
Bsp143I GATC 4 cut(s) 277, 358, 517, 606
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 1 cut(s) 130
BspFNI CGCG 1 cut(s) 126
BspMI ACCTGC 1 cut(s) 379
BspPI GGATC 3 cut(s) 285, 353, 512
BsrI ACTGG 2 cut(s) 51, 316
BssECI CCNNGG 1 cut(s) 235
BssMI GATC 4 cut(s) 277, 358, 517, 606
BssNAI GTATAC 1 cut(s) 394
BssNI GRCGYC 1 cut(s) 578
BssT1I CCWWGG 1 cut(s) 235
Bst1107I GTATAC 1 cut(s) 394
BstACI GRCGYC 1 cut(s) 578
BstDEI CTNAG 1 cut(s) 117
BstF5I GGATG 1 cut(s) 465
BstFNI CGCG 1 cut(s) 126
BstKTI GATC 4 cut(s) 280, 361, 520, 609
BstMAI GTCTC 1 cut(s) 569
BstMBI GATC 4 cut(s) 277, 358, 517, 606
BstNSI RCATGY 1 cut(s) 378
BstUI CGCG 1 cut(s) 126
BstV1I GCAGC 1 cut(s) 460
BstX2I RGATCY 2 cut(s) 277, 517
BstYI RGATCY 2 cut(s) 277, 517
BstZ17I GTATAC 1 cut(s) 394
BsuRI GGCC 1 cut(s) 50
BtgZI GCGATG 1 cut(s) 141
BtsCI GGATG 1 cut(s) 465
BtsIMutI CAGTG 1 cut(s) 58
BveI ACCTGC 1 cut(s) 379
Cfr13I GGNCC 1 cut(s) 56
CviAII CATG 5 cut(s) 375, 416, 539, 598, 711
CviJI RGCY 3 cut(s) 50, 274, 451
CviKI_1 RGCY 3 cut(s) 50, 274, 451
DdeI CTNAG 1 cut(s) 117
DpnI GATC 4 cut(s) 279, 360, 519, 608
DpnII GATC 4 cut(s) 277, 358, 517, 606
EaeI YGGCCR 1 cut(s) 48
Eco130I CCWWGG 1 cut(s) 235
Eco32I GATATC 1 cut(s) 694
Eco47I GGWCC 1 cut(s) 56
Eco57I CTGAAG 1 cut(s) 507
EcoRI GAATTC 1 cut(s) 297
EcoRV GATATC 1 cut(s) 694
EcoT14I CCWWGG 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 235
Esp3I CGTCTC 1 cut(s) 569
FaeI CATG 5 cut(s) 378, 419, 542, 601, 714
FatI CATG 5 cut(s) 374, 415, 538, 597, 710
FblI GTMKAC 1 cut(s) 393
Fnu4HI GCNGC 1 cut(s) 449
FokI GGATG 1 cut(s) 472
Fsp4HI GCNGC 1 cut(s) 449
FspBI CTAG 1 cut(s) 453
GluI GCNGC 1 cut(s) 449
HaeIII GGCC 1 cut(s) 50
Hin1I GRCGYC 1 cut(s) 578
Hin1II CATG 5 cut(s) 378, 419, 542, 601, 714
HinfI GANTC 5 cut(s) 32, 497, 533, 551, 704
Hpy166II GTNNAC 3 cut(s) 56, 321, 394
Hpy188I TCNGA 2 cut(s) 120, 685
Hpy8I GTNNAC 3 cut(s) 56, 321, 394
HpyAV CCTTC 1 cut(s) 285
HpyCH4IV ACGT 2 cut(s) 64, 578
HpyCH4V TGCA 5 cut(s) 4, 131, 287, 368, 641
HpyF3I CTNAG 1 cut(s) 117
HpySE526I ACGT 2 cut(s) 64, 578
Hsp92I GRCGYC 1 cut(s) 578
Hsp92II CATG 5 cut(s) 378, 419, 542, 601, 714
Kzo9I GATC 4 cut(s) 277, 358, 517, 606
LpnPI CCDG 5 cut(s) 64, 233, 260, 329, 374
Lsp1109I GCAGC 1 cut(s) 460
LweI GCATC 3 cut(s) 118, 184, 628
MaeI CTAG 1 cut(s) 453
MaeII ACGT 2 cut(s) 64, 578
MaeIII GTNAC 1 cut(s) 534
MalI GATC 4 cut(s) 279, 360, 519, 608
MboI GATC 4 cut(s) 277, 358, 517, 606
MboII GAAGA 4 cut(s) 35, 527, 656, 677
MflI RGATCY 2 cut(s) 277, 517
MlsI TGGCCA 1 cut(s) 50
MluCI AATT 4 cut(s) 219, 297, 331, 424
MluNI TGGCCA 1 cut(s) 50
MlyI GAGTC 2 cut(s) 542, 698
MmeI TCCRAC 1 cut(s) 550
MnlI CCTC 6 cut(s) 4, 69, 449, 499, 502, 691
Mox20I TGGCCA 1 cut(s) 50
MscI TGGCCA 1 cut(s) 50
MseI TTAA 2 cut(s) 330, 657
MslI CAYNNNNRTG 4 cut(s) 373, 543, 591, 602
Msp20I TGGCCA 1 cut(s) 50
MvnI CGCG 1 cut(s) 126
NdeII GATC 4 cut(s) 277, 358, 517, 606
NlaIII CATG 5 cut(s) 378, 419, 542, 601, 714
NmuCI GTSAC 1 cut(s) 534
NspI RCATGY 1 cut(s) 378
PfeI GAWTC 3 cut(s) 32, 497, 551
PflMI CCANNNNNTGG 1 cut(s) 345
PkrI GCNGC 1 cut(s) 450
PleI GAGTC 2 cut(s) 541, 698
PpsI GAGTC 2 cut(s) 541, 698
PspPI GGNCC 1 cut(s) 56
PsuI RGATCY 2 cut(s) 277, 517
RseI CAYNNNNRTG 4 cut(s) 373, 543, 591, 602
SaqAI TTAA 2 cut(s) 330, 657
SatI GCNGC 1 cut(s) 449
Sau3AI GATC 4 cut(s) 277, 358, 517, 606
Sau96I GGNCC 1 cut(s) 56
SchI GAGTC 2 cut(s) 542, 698
SetI ASST 9 cut(s) 61, 67, 110, 237, 252, 393, 446, 513, 581
SfaNI GCATC 3 cut(s) 118, 184, 628
SinI GGWCC 1 cut(s) 56
SmiMI CAYNNNNRTG 4 cut(s) 373, 543, 591, 602
Sse9I AATT 4 cut(s) 219, 297, 331, 424
SspMI CTAG 1 cut(s) 453
StyI CCWWGG 1 cut(s) 235
TaiI ACGT 2 cut(s) 67, 581
TaqI TCGA 2 cut(s) 549, 696
TasI AATT 4 cut(s) 219, 297, 331, 424
TfiI GAWTC 3 cut(s) 32, 497, 551
Tru1I TTAA 2 cut(s) 330, 657
Tru9I TTAA 2 cut(s) 330, 657
TscAI CASTG 1 cut(s) 58
TseFI GTSAC 1 cut(s) 534
TseI GCWGC 1 cut(s) 448
Tsp45I GTSAC 1 cut(s) 534
TspDTI ATGAA 4 cut(s) 101, 368, 489, 678
TspRI CASTG 1 cut(s) 58
Van91I CCANNNNNTGG 1 cut(s) 345
VpaK11BI GGWCC 1 cut(s) 56
XapI RAATTY 1 cut(s) 297
XceI RCATGY 1 cut(s) 378
XmiI GTMKAC 1 cut(s) 393
XspI CTAG 1 cut(s) 453
ZraI GACGTC 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.