Rh4CG203300

Domain of unknown function (DUF4216)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000178
Physical Location & Seq
Forward (+)
42975357 .. 42976260
904 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG203300.1

Sequence Viewer

Length: 573 bp
ATGGTTTCTCCGGTGGACATTATTGAGCTATCATATCTTGGAAGAAATCAAGTTGTTGTTTTCAAGTGCGATTGGTTCGATCTCACTCAAAAGAGGGGTATCCAAGTTGATGAACATCAATTCACTAGTAAGAATATCAGTAAAACATGGTACACAAACTATCCATTTTCTTTGGCATCTCAGACCCAACAAGTGTACTATTTACGAGATACTAGGCTTGGTAATAATTGGCGTGTCGTAGAGATATCACAACCTCGAGGGAATTATGATGTTGTAGAAAAAGAAGCAAAAGAAAATGAGGTATGTGATTCAGCAGTGCTGGCAGTGGATCCCTATCAACTAGAGTTGCATGATTTTGTCAATCCTATTGAGGTTAATGATGGCTACGTGTCATTAAGTAGGGATGACATGGAAATGATTGTTGTGGATTTGAATGCCAAAAGACAACGAAGAGAAAATGATAGCTTCATTAATGATGATAGTGACGCTGAAGATGGTGGCTCTAATGATGACAATGTTGAGGGTGAATATGTCTCATCTGAGGATGATATTCATGATGCTTATGTTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218

Protein Analysis

190

Amino Acids

21.92

Weight (kDa)

4.26

Isoelectric Point (pI)

34.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4216 PF13952 8 - 80 4.9e-24 Domain of unknown function (DUF4216)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G40087 AT3G30200
fragaria_vesca FvH4_1g23271 FvH4_3g22812 FvH4_3g22813 FvH4_3g31042 FvH4_3g31043 FvH4_7g03653
malus_domestica MD05G1321200.v1.1 MD09G1027200.v1.1 MD15G1434900.v1.1 MD15G1435000.v1.1 MD15G1435100.v1.1
prunus_persica Prupe.1G578800_v2.0.a1 Prupe.1G578800_v2.0.a1 Prupe.1G578800_v2.0.a1 Prupe.2G062700_v2.0.a1 Prupe.5G033400_v2.0.a1 Prupe.7G022900_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1
pyrus_communis pycom03g07600 pycom03g11360 pycom05g02870 pycom111g02130 pycom15g38370 pycom15g38380
rosa_chinensis RchiOBHm_Chr1g0346371 RchiOBHm_Chr4g0402051 RchiOBHm_Chr4g0414991 RchiOBHm_Chr5g0051011 RchiOBHm_Chr5g0051181 RchiOBHm_Chr6g0273071 RchiOBHm_Chr7g0218301 RchiOBHm_Chr7g0241081
rosa_laevigata RLG00000007709 RLG00000008953 RLG00000012400 RLG00000012401 RLG00000013805 RLG00000014199 RLG00000014726 RLG00000017792 RLG00000017793 RLG00000018860 RLG00000020749 RLG00000030708 RLG00000033507
rosa_multiflora Rmu_co8373761.1_g000001 Rmu_sc0000028.1_g000023 Rmu_sc0000235.1_g000040 Rmu_sc0000293.1_g000017 Rmu_sc0000293.1_g000019 Rmu_sc0000651.1_g000012 Rmu_sc0000870.1_g000051 Rmu_sc0001167.1_g000031 Rmu_sc0001373.1_g000039 Rmu_sc0001861.1_g000055 Rmu_sc0001891.1_g000038 Rmu_sc0001909.1_g000015 Rmu_sc0002500.1_g000002 Rmu_sc0002868.1_g000036 Rmu_sc0002868.1_g000038 Rmu_sc0002868.1_g000039 Rmu_sc0003379.1_g000001 Rmu_sc0003778.1_g000005 Rmu_sc0003778.1_g000006 Rmu_sc0010475.1_g000011 Rmu_sc0015271.1_g000002 Rmu_sc0015271.1_g000003 Rmu_sc0017962.1_g000001 Rmu_sc0017962.1_g000003 Rmu_sc0022129.1_g000004 Rmu_sc0025694.1_g000003 Rmu_sc0025694.1_g000004
rosa_roxburghii Rroxscaffold_1G00031640 Rroxscaffold_1G00047620 Rroxscaffold_1G00047630 Rroxscaffold_1G00050560 Rroxscaffold_1G00050570 Rroxscaffold_1G00054800 Rroxscaffold_1G00055890 Rroxscaffold_2G00108230 Rroxscaffold_3G00240860 Rroxscaffold_4G00299670 Rroxscaffold_4G00299680 Rroxscaffold_4G00299690 Rroxscaffold_4G00306320 Rroxscaffold_4G00306340 Rroxscaffold_5G00341060 Rroxscaffold_5G00342000 Rroxscaffold_5G00355660 Rroxscaffold_5G00357170 Rroxscaffold_6G00399490 Rroxscaffold_6G00408400 Rroxscaffold_7G00189260 Rroxscaffold_7G00199620 Rroxscaffold_7G00215470
rosa_rugosa Rorug02G0117600 Rorug02G0117700 Rorug04G0116400 Rorug06G0103200
rosa_samantha Rh1AG062800 Rh1AG073700 Rh1CG091700 Rh1DG077600 Rh1DG077700 Rh1DG077800 Rh1DG117400 Rh1DG146300 Rh2BG193300 Rh2BG193400 Rh2BG265300 Rh2BG413400 Rh3CG365500 Rh4AG315400 Rh4BG323100 Rh4BG323200 Rh4CG071400 Rh4CG142500 Rh4CG203300 Rh4CG338300 Rh4DG079300 Rh4DG079400 Rh4DG217000 Rh4DG217100 Rh4DG217200 Rh4DG217300 Rh5AG128300 Rh5AG239900 Rh5AG465900 Rh5BG348600 Rh5BG387900 Rh5BG388000 Rh5CG181800 Rh5CG181900 Rh5CG271000 Rh5CG271100 Rh5DG127100 Rh5DG138400 Rh5DG168200 Rh5DG168300 Rh5DG361500 Rh5DG460400 Rh6AG045400 Rh6AG058600 Rh6BG275000 Rh6CG089700 Rh6DG182300 Rh6DG182400 Rh6DG207300 Rh6DG207400 Rh7BG374000 Rh7BG390600 Rh7BG390700 Rh7BG390800
rosa_wichuraiana Rw0G018690 Rw1G007320 Rw4G008510 Rw5G011510 Rw5G021830 Rw6G024150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 255
AclWI GGATC 2 cut(s) 323, 336
AcuI CTGAAG 1 cut(s) 510
AfaI GTAC 2 cut(s) 152, 197
AflIII ACRYGT 1 cut(s) 387
AgsI TTSAA 2 cut(s) 64, 433
AhlI ACTAGT 1 cut(s) 125
AluBI AGCT 2 cut(s) 28, 465
AluI AGCT 2 cut(s) 28, 465
Alw26I GTCTC 1 cut(s) 538
AlwI GGATC 2 cut(s) 323, 336
Ama87I CYCGRG 1 cut(s) 255
AseI ATTAAT 1 cut(s) 471
AsuHPI GGTGA 1 cut(s) 536
AvaI CYCGRG 1 cut(s) 255
BamHI GGATCC 1 cut(s) 328
BccI CCATC 2 cut(s) 374, 488
BciVI GTATCC 1 cut(s) 110
BcoDI GTCTC 1 cut(s) 538
BcuI ACTAGT 1 cut(s) 125
BfaI CTAG 3 cut(s) 126, 213, 341
BfuI GTATCC 1 cut(s) 110
BmeT110I CYCGRG 1 cut(s) 255
BmiI GGNNCC 1 cut(s) 330
BmsI GCATC 2 cut(s) 185, 547
BsaAI YACGTR 1 cut(s) 388
BsaBI GATNNNNATC 2 cut(s) 114, 333
BsaWI WCCGGW 1 cut(s) 10
Bse8I GATNNNNATC 2 cut(s) 114, 333
BseGI GGATG 2 cut(s) 409, 550
BseJI GATNNNNATC 2 cut(s) 114, 333
BseMII CTCAG 2 cut(s) 194, 531
BsiHKCI CYCGRG 1 cut(s) 255
BsiSI CCGG 1 cut(s) 11
BsmAI GTCTC 1 cut(s) 538
BsmI GAATGC 1 cut(s) 439
BsoBI CYCGRG 1 cut(s) 255
Bsp143I GATC 2 cut(s) 79, 328
BspCNI CTCAG 2 cut(s) 193, 532
BspHI TCATGA 1 cut(s) 553
BspLI GGNNCC 1 cut(s) 330
BspPI GGATC 2 cut(s) 323, 336
BssMI GATC 2 cut(s) 79, 328
Bst6I CTCTTC 1 cut(s) 445
BstBAI YACGTR 1 cut(s) 388
BstC8I GCNNGC 1 cut(s) 321
BstDEI CTNAG 2 cut(s) 180, 540
BstF5I GGATG 2 cut(s) 409, 550
BstKTI GATC 2 cut(s) 82, 331
BstMAI GTCTC 1 cut(s) 538
BstMBI GATC 2 cut(s) 79, 328
BstMWI GCNNNNNNNGC 1 cut(s) 320
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
BsuI GTATCC 1 cut(s) 110
BtsCI GGATG 2 cut(s) 409, 550
BtsI GCAGTG 2 cut(s) 321, 330
BtsIMutI CAGTG 2 cut(s) 321, 330
Cac8I GCNNGC 1 cut(s) 321
CciI TCATGA 1 cut(s) 553
CseI GACGC 1 cut(s) 494
Csp6I GTAC 2 cut(s) 151, 196
CviAII CATG 4 cut(s) 147, 350, 409, 554
CviJI RGCY 5 cut(s) 28, 217, 384, 465, 501
CviKI_1 RGCY 5 cut(s) 28, 217, 384, 465, 501
CviQI GTAC 2 cut(s) 151, 196
DdeI CTNAG 2 cut(s) 180, 540
DpnI GATC 2 cut(s) 81, 330
DpnII GATC 2 cut(s) 79, 328
Eam1104I CTCTTC 1 cut(s) 445
EarI CTCTTC 1 cut(s) 445
Eco32I GATATC 1 cut(s) 246
Eco57I CTGAAG 1 cut(s) 510
Eco88I CYCGRG 1 cut(s) 255
EcoRV GATATC 1 cut(s) 246
FaeI CATG 4 cut(s) 150, 353, 412, 557
FatI CATG 4 cut(s) 146, 349, 408, 553
FokI GGATG 2 cut(s) 416, 557
FspBI CTAG 3 cut(s) 126, 213, 341
HapII CCGG 1 cut(s) 11
HgaI GACGC 1 cut(s) 494
Hin1II CATG 4 cut(s) 150, 353, 412, 557
HinfI GANTC 1 cut(s) 308
HpaII CCGG 1 cut(s) 11
HphI GGTGA 1 cut(s) 536
Hpy166II GTNNAC 3 cut(s) 16, 153, 196
Hpy188I TCNGA 2 cut(s) 183, 541
Hpy188III TCNNGA 1 cut(s) 554
Hpy8I GTNNAC 3 cut(s) 16, 153, 196
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 1 cut(s) 349
HpyF10VI GCNNNNNNNGC 1 cut(s) 320
HpyF3I CTNAG 2 cut(s) 180, 540
HpySE526I ACGT 1 cut(s) 387
Hsp92II CATG 4 cut(s) 150, 353, 412, 557
Kzo9I GATC 2 cut(s) 79, 328
LpnPI CCDG 2 cut(s) 24, 305
LweI GCATC 2 cut(s) 185, 547
MaeI CTAG 3 cut(s) 126, 213, 341
MaeII ACGT 1 cut(s) 387
MaeIII GTNAC 1 cut(s) 482
MalI GATC 2 cut(s) 81, 330
MboI GATC 2 cut(s) 79, 328
MboII GAAGA 3 cut(s) 54, 462, 503
MflI RGATCY 1 cut(s) 328
MluCI AATT 3 cut(s) 119, 226, 262
MnlI CCTC 7 cut(s) 87, 251, 264, 292, 364, 514, 535
MseI TTAA 3 cut(s) 375, 395, 471
MslI CAYNNNNRTG 1 cut(s) 413
MspI CCGG 1 cut(s) 11
Mva1269I GAATGC 1 cut(s) 439
MwoI GCNNNNNNNGC 1 cut(s) 320
NdeII GATC 2 cut(s) 79, 328
NlaIII CATG 4 cut(s) 150, 353, 412, 557
NlaIV GGNNCC 1 cut(s) 330
NmuCI GTSAC 1 cut(s) 482
PaeR7I CTCGAG 1 cut(s) 255
PagI TCATGA 1 cut(s) 553
PctI GAATGC 1 cut(s) 439
PfeI GAWTC 1 cut(s) 308
Ppu21I YACGTR 1 cut(s) 388
PshBI ATTAAT 1 cut(s) 471
PspN4I GGNNCC 1 cut(s) 330
PspXI VCTCGAGB 1 cut(s) 255
PsuI RGATCY 1 cut(s) 328
RsaI GTAC 2 cut(s) 152, 197
RsaNI GTAC 2 cut(s) 151, 196
RseI CAYNNNNRTG 1 cut(s) 413
SaqAI TTAA 3 cut(s) 375, 395, 471
Sau3AI GATC 2 cut(s) 79, 328
SetI ASST 6 cut(s) 30, 256, 303, 375, 390, 467
SfaNI GCATC 2 cut(s) 185, 547
Sfr274I CTCGAG 1 cut(s) 255
SlaI CTCGAG 1 cut(s) 255
SmiMI CAYNNNNRTG 1 cut(s) 413
SmlI CTYRAG 1 cut(s) 255
SmoI CTYRAG 1 cut(s) 255
SpeI ACTAGT 1 cut(s) 125
Sse9I AATT 3 cut(s) 119, 226, 262
SspMI CTAG 3 cut(s) 126, 213, 341
TaiI ACGT 1 cut(s) 390
TaqI TCGA 2 cut(s) 78, 256
TasI AATT 3 cut(s) 119, 226, 262
TatI WGTACW 1 cut(s) 195
TfiI GAWTC 1 cut(s) 308
Tru1I TTAA 3 cut(s) 375, 395, 471
Tru9I TTAA 3 cut(s) 375, 395, 471
TscAI CASTG 2 cut(s) 321, 330
TseFI GTSAC 1 cut(s) 482
Tsp45I GTSAC 1 cut(s) 482
TspDTI ATGAA 3 cut(s) 126, 457, 542
TspRI CASTG 2 cut(s) 321, 330
VspI ATTAAT 1 cut(s) 471
XhoI CTCGAG 1 cut(s) 255
XspI CTAG 3 cut(s) 126, 213, 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.