Rh4AG315400

Domain of unknown function (DUF4216)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000178
Physical Location & Seq
Reverse (-)
62555252 .. 62578386
23135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG315400.1

Sequence Viewer

Length: 423 bp
ATGACAGGAATATCTGTTTTCAACCAGAATGTTAGGTTTTTAAAAGGAGCAGTTGATGATGTTCTTAGCCTAACTGATTTTGCAATGATTCGATGGTATGTGCTGAACAATTGTGATGAGGTACTCCCATATATAAGGGAGCATAAAGCAGAACTTGAGAGGCAGAATATTACAAACATAGGAAAGGAACAGCAACAAAGATTTCATAAGTGGTTTTTGAGACGTGTTCAACAGATGCAAGTTGAGGGTTCAACAGAACATATCGAATCCTTACTAAACTTGGCCAGTGGACCTCAGCGTGAAGTGACACGGTATAGTGGGTGTGTTGTCAATGGCATAAGGTTTCATACTCAGAAACGCGATGGAAATAAAAAAACTCAAAATAATGGAGTGGTAGTCAAAGGGGAGCATCGCGGTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218

Protein Analysis

140

Amino Acids

16.27

Weight (kDa)

9.46

Isoelectric Point (pI)

34.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G40087 AT3G30200
fragaria_vesca FvH4_1g23271 FvH4_3g22812 FvH4_3g22813 FvH4_3g31042 FvH4_3g31043 FvH4_7g03653
malus_domestica MD05G1321200.v1.1 MD09G1027200.v1.1 MD15G1434900.v1.1 MD15G1435000.v1.1 MD15G1435100.v1.1
prunus_persica Prupe.1G578800_v2.0.a1 Prupe.1G578800_v2.0.a1 Prupe.1G578800_v2.0.a1 Prupe.2G062700_v2.0.a1 Prupe.5G033400_v2.0.a1 Prupe.7G022900_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1 Prupe.7G105300_v2.0.a1
pyrus_communis pycom03g07600 pycom03g11360 pycom05g02870 pycom111g02130 pycom15g38370 pycom15g38380
rosa_chinensis RchiOBHm_Chr1g0346371 RchiOBHm_Chr4g0402051 RchiOBHm_Chr4g0414991 RchiOBHm_Chr5g0051011 RchiOBHm_Chr5g0051181 RchiOBHm_Chr6g0273071 RchiOBHm_Chr7g0218301 RchiOBHm_Chr7g0241081
rosa_laevigata RLG00000007709 RLG00000008953 RLG00000012400 RLG00000012401 RLG00000013805 RLG00000014199 RLG00000014726 RLG00000017792 RLG00000017793 RLG00000018860 RLG00000020749 RLG00000030708 RLG00000033507
rosa_multiflora Rmu_co8373761.1_g000001 Rmu_sc0000028.1_g000023 Rmu_sc0000235.1_g000040 Rmu_sc0000293.1_g000017 Rmu_sc0000293.1_g000019 Rmu_sc0000651.1_g000012 Rmu_sc0000870.1_g000051 Rmu_sc0001167.1_g000031 Rmu_sc0001373.1_g000039 Rmu_sc0001861.1_g000055 Rmu_sc0001891.1_g000038 Rmu_sc0001909.1_g000015 Rmu_sc0002500.1_g000002 Rmu_sc0002868.1_g000036 Rmu_sc0002868.1_g000038 Rmu_sc0002868.1_g000039 Rmu_sc0003379.1_g000001 Rmu_sc0003778.1_g000005 Rmu_sc0003778.1_g000006 Rmu_sc0010475.1_g000011 Rmu_sc0015271.1_g000002 Rmu_sc0015271.1_g000003 Rmu_sc0017962.1_g000001 Rmu_sc0017962.1_g000003 Rmu_sc0022129.1_g000004 Rmu_sc0025694.1_g000003 Rmu_sc0025694.1_g000004
rosa_roxburghii Rroxscaffold_1G00031640 Rroxscaffold_1G00047620 Rroxscaffold_1G00047630 Rroxscaffold_1G00050560 Rroxscaffold_1G00050570 Rroxscaffold_1G00054800 Rroxscaffold_1G00055890 Rroxscaffold_2G00108230 Rroxscaffold_3G00240860 Rroxscaffold_4G00299670 Rroxscaffold_4G00299680 Rroxscaffold_4G00299690 Rroxscaffold_4G00306320 Rroxscaffold_4G00306340 Rroxscaffold_5G00341060 Rroxscaffold_5G00342000 Rroxscaffold_5G00355660 Rroxscaffold_5G00357170 Rroxscaffold_6G00399490 Rroxscaffold_6G00408400 Rroxscaffold_7G00189260 Rroxscaffold_7G00199620 Rroxscaffold_7G00215470
rosa_rugosa Rorug02G0117600 Rorug02G0117700 Rorug04G0116400 Rorug06G0103200
rosa_samantha Rh1AG062800 Rh1AG073700 Rh1CG091700 Rh1DG077600 Rh1DG077700 Rh1DG077800 Rh1DG117400 Rh1DG146300 Rh2BG193300 Rh2BG193400 Rh2BG265300 Rh2BG413400 Rh3CG365500 Rh4AG315400 Rh4BG323100 Rh4BG323200 Rh4CG071400 Rh4CG142500 Rh4CG203300 Rh4CG338300 Rh4DG079300 Rh4DG079400 Rh4DG217000 Rh4DG217100 Rh4DG217200 Rh4DG217300 Rh5AG128300 Rh5AG239900 Rh5AG465900 Rh5BG348600 Rh5BG387900 Rh5BG388000 Rh5CG181800 Rh5CG181900 Rh5CG271000 Rh5CG271100 Rh5DG127100 Rh5DG138400 Rh5DG168200 Rh5DG168300 Rh5DG361500 Rh5DG460400 Rh6AG045400 Rh6AG058600 Rh6BG275000 Rh6CG089700 Rh6DG182300 Rh6DG182400 Rh6DG207300 Rh6DG207400 Rh7BG374000 Rh7BG390600 Rh7BG390700 Rh7BG390800
rosa_wichuraiana Rw0G018690 Rw1G007320 Rw4G008510 Rw5G011510 Rw5G021830 Rw6G024150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 360, 414
AciI CCGC 1 cut(s) 414
AcoI YGGCCR 1 cut(s) 282
AfaI GTAC 1 cut(s) 123
AflIII ACRYGT 1 cut(s) 223
AgsI TTSAA 3 cut(s) 22, 230, 252
AjiI CACGTC 1 cut(s) 224
Alw26I GTCTC 1 cut(s) 214
AoxI GGCC 1 cut(s) 282
AspS9I GGNCC 1 cut(s) 290
AvaII GGWCC 1 cut(s) 290
BalI TGGCCA 1 cut(s) 284
BbvCI CCTCAGC 1 cut(s) 294
BccI CCATC 2 cut(s) 87, 356
BcoDI GTCTC 1 cut(s) 214
BfaI CTAG 1 cut(s) 421
Bme18I GGWCC 1 cut(s) 290
BmgBI CACGTC 1 cut(s) 224
BmgT120I GGNCC 1 cut(s) 290
BmsI GCATC 2 cut(s) 225, 418
Bpu10I CCTNAGC 1 cut(s) 294
BpuEI CTTGAG 1 cut(s) 176
BsaXI ACNNNNNCTCC 2 cut(s) 381, 411
Bse1I ACTGG 1 cut(s) 285
Bse3DI GCAATG 1 cut(s) 90
BseMI GCAATG 1 cut(s) 90
BseMII CTCAG 2 cut(s) 308, 365
BseNI ACTGG 1 cut(s) 285
Bsh1236I CGCG 2 cut(s) 360, 414
BshFI GGCC 1 cut(s) 284
BsmAI GTCTC 1 cut(s) 214
BsmBI CGTCTC 1 cut(s) 214
BsnI GGCC 1 cut(s) 284
BspACI CCGC 1 cut(s) 414
BspANI GGCC 1 cut(s) 284
BspCNI CTCAG 2 cut(s) 307, 364
BspFNI CGCG 2 cut(s) 360, 414
BsrDI GCAATG 1 cut(s) 90
BsrI ACTGG 1 cut(s) 285
Bst4CI ACNGT 1 cut(s) 312
BstDEI CTNAG 3 cut(s) 65, 294, 351
BstFNI CGCG 2 cut(s) 360, 414
BstMAI GTCTC 1 cut(s) 214
BstUI CGCG 2 cut(s) 360, 414
BsuRI GGCC 1 cut(s) 284
BtgZI GCGATG 2 cut(s) 375, 395
BtrI CACGTC 1 cut(s) 224
BtsIMutI CAGTG 1 cut(s) 292
Cfr13I GGNCC 1 cut(s) 290
Csp6I GTAC 1 cut(s) 122
CviJI RGCY 2 cut(s) 69, 284
CviKI_1 RGCY 2 cut(s) 69, 284
CviQI GTAC 1 cut(s) 122
DdeI CTNAG 3 cut(s) 65, 294, 351
DraI TTTAAA 1 cut(s) 42
EaeI YGGCCR 1 cut(s) 282
Eco47I GGWCC 1 cut(s) 290
Esp3I CGTCTC 1 cut(s) 214
FalI AAGNNNNNCTT 2 cut(s) 138, 170
FspBI CTAG 1 cut(s) 421
HaeIII GGCC 1 cut(s) 284
HinfI GANTC 2 cut(s) 88, 266
Hpy166II GTNNAC 1 cut(s) 290
Hpy188I TCNGA 1 cut(s) 354
Hpy8I GTNNAC 1 cut(s) 290
HpyCH4III ACNGT 1 cut(s) 312
HpyCH4IV ACGT 1 cut(s) 223
HpyCH4V TGCA 2 cut(s) 83, 238
HpyF3I CTNAG 3 cut(s) 65, 294, 351
HpySE526I ACGT 1 cut(s) 223
LmnI GCTCC 3 cut(s) 47, 139, 406
LpnPI CCDG 2 cut(s) 38, 298
LweI GCATC 2 cut(s) 225, 418
MaeI CTAG 1 cut(s) 421
MaeII ACGT 1 cut(s) 223
MaeIII GTNAC 1 cut(s) 304
MfeI CAATTG 1 cut(s) 109
MlsI TGGCCA 1 cut(s) 284
MluCI AATT 1 cut(s) 109
MluNI TGGCCA 1 cut(s) 284
MnlI CCTC 4 cut(s) 112, 153, 238, 303
Mox20I TGGCCA 1 cut(s) 284
MscI TGGCCA 1 cut(s) 284
MseI TTAA 1 cut(s) 41
Msp20I TGGCCA 1 cut(s) 284
MunI CAATTG 1 cut(s) 109
MvnI CGCG 2 cut(s) 360, 414
NmuCI GTSAC 1 cut(s) 304
PfeI GAWTC 2 cut(s) 88, 266
PspPI GGNCC 1 cut(s) 290
RsaI GTAC 1 cut(s) 123
RsaNI GTAC 1 cut(s) 122
SaqAI TTAA 1 cut(s) 41
Sau96I GGNCC 1 cut(s) 290
SetI ASST 5 cut(s) 38, 123, 226, 295, 344
SfaNI GCATC 2 cut(s) 225, 418
SinI GGWCC 1 cut(s) 290
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 1 cut(s) 109
SsiI CCGC 1 cut(s) 414
SspI AATATT 1 cut(s) 169
SspMI CTAG 1 cut(s) 421
TaaI ACNGT 1 cut(s) 312
TaiI ACGT 1 cut(s) 226
TaqI TCGA 2 cut(s) 91, 264
TasI AATT 1 cut(s) 109
TfiI GAWTC 2 cut(s) 88, 266
Tru1I TTAA 1 cut(s) 41
Tru9I TTAA 1 cut(s) 41
TscAI CASTG 1 cut(s) 292
TseFI GTSAC 1 cut(s) 304
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 2 cut(s) 194, 335
TspRI CASTG 1 cut(s) 292
VpaK11BI GGWCC 1 cut(s) 290
XspI CTAG 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.