FvH4_4g01350

Perakine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000149
Physical Location & Seq
Reverse (-)
1249499 .. 1252368
2870 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g01350.t4

Sequence Viewer

Length: 1035 bp
ATGGAGGACAACTCTCGTATTCAAATCCCAAAGATTAAACTGGGTAGTCAGGGATTGGAGGTTTCTAGATTGGGTTTTGGATGTGGTGGACTATCTGGAATATACAATGCTCCTCTCTCTCATGAAGCTGGATGTTCAGTGATAAACGAAGCATTTGATAGGGGCATTACCTTCTATGACACTTCGGACCTTTATGGTGACAATCATGATAATGAATTCTTGGTTGGCAAGGCTTTGAAACAGCTTCCTCGAGAAAAAGTCCAACTGGCAACAAAATGTGGCCTTGTCAGGTCAGACGGCTTTCAGTTCACGGCAAATGGTACCCCGGAATATGTAAGAGCATGCTGCGAAGCTAGTCTAAAGAGGCTGGATGTCGACTATATTGATCTGTACTATTTGCACCGTATCGATGTTTCAGTGCCAATTGAGGATACTATTGGGGAGCTTAAGAAGCTTGTGCATGAAGGAAAGATTAGATACATTGGTCTATCAGAAGCTAGTATAGACACCATAATGAGAGCTCATGCTGTTCATCCCATCACTGCTGTGCAAATGGAGTATTCACTGTGGACTCGAGACATTGAAAATGAAATAATTCCACTTTGCCGGAAGCTTGGAATTGGGATTGTGTCATATAGTCCTCTCGGTCGTGGTTTCTTTGGTGGGAAGGCAGTTCTGGAGAGCTTGCCTGCAGAAAGTTTGTTGTCTATTCATCCAAGGTTCAATGGCAAAAATCTGGAAAAGAACAAACTTTTCTATAACAAGCTTGCCAATCTTGCTGCGAAGCATTCATGCAATGCTCCTCAGTTAGCTCTGTCATGGCTTCTCCATCAGGGCAAGGACATAATCCCGATCCCAGGGACTACTAAACTTAAGAACCTGGATATCAATGTCGGATCGTTGGAAGTGAAGCTTACAAAAGAGGATTTGAATGAAATTTGTGATGCTGTTCCCATTGATGAAATTGGAGGAGACCGAGAATATGAAGTATTCTCAAAGTTTGTGTGGAGTAATGCAAGTACACCATCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

345

Amino Acids

38.1

Weight (kDa)

6.02

Isoelectric Point (pI)

25.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 23 - 287 1e-63 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000149)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g01350 FvH4_4g01350 FvH4_4g01350 FvH4_4g01350 FvH4_4g01350 FvH4_4g01350 FvH4_4g01350 FvH4_4g01550 FvH4_4g01550 FvH4_4g01550 FvH4_6g03001 FvH4_6g03010 FvH4_6g03030 FvH4_6g03050 FvH4_6g03050 FvH4_6g03050 FvH4_6g03051 FvH4_6g03051 FvH4_6g03051 FvH4_6g03051 FvH4_6g03052 FvH4_6g03070 FvH4_6g03070 FvH4_6g03110
malus_domestica MD04G1223200.v1.1 MD08G1240600.v1.1 MD12G1239000.v1.1 MD12G1239100.v1.1 MD12G1239200.v1.1 MD12G1239300.v1.1 MD12G1239400.v1.1 MD12G1239600.v1.1 MD12G1239800.v1.1 MD12G1240000.v1.1 MD12G1240300.v1.1 MD12G1240400.v1.1 MD13G1200700.v1.1
prunus_persica Prupe.1G021900_v2.0.a1 Prupe.1G022000_v2.0.a1 Prupe.6G141300_v2.0.a1 Prupe.6G141300_v2.0.a1 Prupe.6G141300_v2.0.a1 Prupe.6G341900_v2.0.a1 Prupe.6G342100_v2.0.a1 Prupe.6G342200_v2.0.a1 Prupe.6G342300_v2.0.a1
pyrus_communis pycom04g19710 pycom12g21970 pycom12g21980 pycom12g21990 pycom12g22000 pycom12g22020 pycom13g17420 pycom16g16880
rosa_chinensis RchiOBHm_Chr3g0450451 RchiOBHm_Chr3g0451211 RchiOBHm_Chr3g0451221 RchiOBHm_Chr3g0451251 RchiOBHm_Chr3g0451261 RchiOBHm_Chr3g0451281 RchiOBHm_Chr3g0451321 RchiOBHm_Chr3g0451331 RchiOBHm_Chr3g0451341 RchiOBHm_Chr3g0454931 RchiOBHm_Chr4g0387811 RchiOBHm_Chr4g0387821 RchiOBHm_Chr4g0388101
rosa_laevigata RLG00000010080 RLG00000010092 RLG00000010093 RLG00000025402 RLG00000025666 RLG00000025667 RLG00000025668 RLG00000025669 RLG00000025672 RLG00000025673 RLG00000025674 RLG00000025675 RLG00000025676 RLG00000025678 RLG00000025679 RLG00000025729
rosa_multiflora Rmu_co8283913.1_g000001 Rmu_co8296899.1_g000001 Rmu_sc0000330.1_g000014 Rmu_sc0001792.1_g000004 Rmu_sc0002169.1_g000002 Rmu_sc0002180.1_g000028 Rmu_sc0002509.1_g000009 Rmu_sc0002575.1_g000011 Rmu_sc0002575.1_g000013 Rmu_sc0002575.1_g000015 Rmu_sc0002575.1_g000019 Rmu_sc0002575.1_g000021 Rmu_sc0002575.1_g000022 Rmu_sc0012229.1_g000010 Rmu_sc0012229.1_g000014 Rmu_sc0012229.1_g000017 Rmu_sc0012229.1_g000018 Rmu_sc0013958.1_g000001 Rmu_sc0030279.1_g000001 Rmu_sc0031850.1_g000002
rosa_roxburghii Rroxscaffold_163G00450840 Rroxscaffold_163G00450860 Rroxscaffold_163G00450870 Rroxscaffold_164G00436570 Rroxscaffold_5G00334430 Rroxscaffold_5G00334440 Rroxscaffold_5G00334650 Rroxscaffold_6G00428150 Rroxscaffold_6G00428660 Rroxscaffold_6G00428680 Rroxscaffold_6G00428710 Rroxscaffold_6G00428720 Rroxscaffold_6G00428730 Rroxscaffold_6G00428740 Rroxscaffold_6G00428750 Rroxscaffold_6G00428760
rosa_rugosa Rorug02G0535300 Rorug02G0627900 Rorug02G0632500 Rorug02G0632600 Rorug02G0633000 Rorug02G0633100 Rorug03G0003000 Rorug03G0313700 Rorug03G0314800 Rorug05G0229300 Rorug07G0155500
rosa_samantha Rh3AG028500 Rh3AG035300 Rh3AG035400 Rh3AG035500 Rh3AG035600 Rh3AG035700 Rh3AG035800 Rh3AG035900 Rh3AG062900 Rh3BG029000 Rh3BG035500 Rh3BG035600 Rh3BG035800 Rh3BG035900 Rh3BG036000 Rh3BG036100 Rh3BG036200 Rh3BG036300 Rh3BG036400 Rh3BG036500 Rh3BG036600 Rh3BG064800 Rh3CG027800 Rh3CG034200 Rh3CG034400 Rh3CG034500 Rh3CG034600 Rh3CG034700 Rh3CG034800 Rh3CG034900 Rh3CG063700 Rh3DG035100 Rh3DG035200 Rh3DG035400 Rh3DG035500 Rh3DG035600 Rh3DG035700 Rh3DG035800 Rh3DG035900 Rh3DG036000 Rh3DG036100 Rh3DG064800 Rh4AG018700 Rh4BG013600 Rh4BG013700 Rh4BG014900 Rh4CG021200 Rh4DG015600
rosa_wichuraiana Rw0G001850 Rw3G002200 Rw3G002580 Rw3G002590 Rw3G002610 Rw3G002620 Rw3G002630 Rw3G002640 Rw3G002650 Rw3G002660 Rw3G004920 Rw3G016120 Rw4G001220 Rw4G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 320
AccB1I GGYRCC 1 cut(s) 320
AccI GTMKAC 1 cut(s) 375
AclWI GGATC 2 cut(s) 847, 904
AcsI RAATTY 2 cut(s) 215, 936
AfaI GTAC 3 cut(s) 322, 392, 1021
AfiI CCNNNNNNNGG 1 cut(s) 857
AflII CTTAAG 2 cut(s) 446, 872
AgsI TTSAA 5 cut(s) 23, 238, 584, 724, 931
AjnI CCWGG 2 cut(s) 856, 879
AjuI GAANNNNNNNTTGG 2 cut(s) 207, 239
AleI CACNNNNGTG 1 cut(s) 545
Alw21I GWGCWC 1 cut(s) 523
Alw26I GTCTC 2 cut(s) 570, 966
AlwI GGATC 2 cut(s) 847, 904
Ama87I CYCGRG 2 cut(s) 249, 573
AoxI GGCC 1 cut(s) 280
ApeKI GCWGC 2 cut(s) 345, 779
ApoI RAATTY 2 cut(s) 215, 936
Asp700I GAANNNNTTC 1 cut(s) 594
Asp718I GGTACC 1 cut(s) 320
AspS9I GGNCC 1 cut(s) 187
AsuC2I CCSGG 1 cut(s) 326
AsuHPI GGTGA 1 cut(s) 209
AvaI CYCGRG 2 cut(s) 249, 573
AvaII GGWCC 1 cut(s) 187
BaeI ACNNNNGTAYC 2 cut(s) 469, 502
BanI GGYRCC 1 cut(s) 320
BanII GRGCYC 1 cut(s) 523
Bbv12I GWGCWC 1 cut(s) 523
BbvI GCAGC 2 cut(s) 332, 766
BccI CCATC 3 cut(s) 545, 837, 1033
BceAI ACGGC 2 cut(s) 313, 327
BciT130I CCWGG 2 cut(s) 858, 881
BciVI GTATCC 1 cut(s) 424
BcnI CCSGG 1 cut(s) 326
BcoDI GTCTC 2 cut(s) 570, 966
BfaI CTAG 3 cut(s) 66, 354, 498
BfmI CTRYAG 1 cut(s) 690
BfrI CTTAAG 2 cut(s) 446, 872
BfuI GTATCC 1 cut(s) 424
BisI GCNGC 2 cut(s) 346, 780
BlsI GCNGC 2 cut(s) 347, 781
Bme1390I CCNGG 3 cut(s) 326, 858, 881
Bme18I GGWCC 1 cut(s) 187
BmeT110I CYCGRG 2 cut(s) 249, 573
BmgT120I GGNCC 1 cut(s) 187
BmiI GGNNCC 1 cut(s) 322
BmrFI CCNGG 3 cut(s) 326, 858, 881
BmrI ACTGGG 1 cut(s) 50
BmsI GCATC 1 cut(s) 934
BmuI ACTGGG 1 cut(s) 50
BplI GAGNNNNNCTC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 698
BpuMI CCSGG 1 cut(s) 326
Bsa29I ATCGAT 1 cut(s) 408
BsaI GGTCTC 1 cut(s) 966
BsaJI CCNNGG 4 cut(s) 324, 716, 856, 857
Bsc4I CCNNNNNNNGG 1 cut(s) 857
Bse1I ACTGG 2 cut(s) 45, 270
Bse3DI GCAATG 1 cut(s) 802
BseBI CCWGG 2 cut(s) 858, 881
BseCI ATCGAT 1 cut(s) 408
BseDI CCNNGG 4 cut(s) 324, 716, 856, 857
BseGI GGATG 5 cut(s) 86, 137, 376, 532, 712
BseLI CCNNNNNNNGG 1 cut(s) 857
BseMI GCAATG 1 cut(s) 802
BseMII CTCAG 1 cut(s) 818
BseNI ACTGG 2 cut(s) 45, 270
BseRI GAGGAG 3 cut(s) 102, 792, 984
BseXI GCAGC 2 cut(s) 332, 766
Bsh1285I CGRYCG 1 cut(s) 649
BshFI GGCC 1 cut(s) 282
BshNI GGYRCC 1 cut(s) 320
BshVI ATCGAT 1 cut(s) 408
BsiEI CGRYCG 1 cut(s) 649
BsiHKAI GWGCWC 1 cut(s) 523
BsiHKCI CYCGRG 2 cut(s) 249, 573
BsiSI CCGG 2 cut(s) 326, 607
BslFI GGGAC 1 cut(s) 874
BslI CCNNNNNNNGG 1 cut(s) 857
BsmAI GTCTC 2 cut(s) 570, 966
BsmFI GGGAC 1 cut(s) 874
BsmI GAATGC 1 cut(s) 787
BsnI GGCC 1 cut(s) 282
Bso31I GGTCTC 1 cut(s) 966
BsoBI CYCGRG 2 cut(s) 249, 573
Bsp1286I GDGCHC 1 cut(s) 523
Bsp143I GATC 3 cut(s) 385, 852, 896
BspANI GGCC 1 cut(s) 282
BspCNI CTCAG 1 cut(s) 817
BspDI ATCGAT 1 cut(s) 408
BspHI TCATGA 2 cut(s) 121, 205
BspLI GGNNCC 1 cut(s) 322
BspMAI CTGCAG 1 cut(s) 694
BspPI GGATC 2 cut(s) 847, 904
BspT107I GGYRCC 1 cut(s) 320
BspTI CTTAAG 2 cut(s) 446, 872
BspTNI GGTCTC 1 cut(s) 966
BsrDI GCAATG 1 cut(s) 802
BsrI ACTGG 2 cut(s) 45, 270
BssECI CCNNGG 4 cut(s) 324, 716, 856, 857
BssMI GATC 3 cut(s) 385, 852, 896
BssT1I CCWWGG 1 cut(s) 716
Bst2UI CCWGG 2 cut(s) 858, 881
Bst4CI ACNGT 2 cut(s) 404, 567
BstAFI CTTAAG 2 cut(s) 446, 872
BstC8I GCNNGC 4 cut(s) 343, 686, 690, 768
BstDEI CTNAG 1 cut(s) 804
BstF5I GGATG 5 cut(s) 86, 137, 376, 532, 712
BstKTI GATC 3 cut(s) 388, 855, 899
BstMAI GTCTC 2 cut(s) 570, 966
BstMBI GATC 3 cut(s) 385, 852, 896
BstMCI CGRYCG 1 cut(s) 649
BstMWI GCNNNNNNNGC 2 cut(s) 451, 776
BstNI CCWGG 2 cut(s) 858, 881
BstNSI RCATGY 1 cut(s) 345
BstSCI CCNGG 3 cut(s) 324, 856, 879
BstSFI CTRYAG 1 cut(s) 690
BstV1I GCAGC 2 cut(s) 332, 766
Bsu15I ATCGAT 1 cut(s) 408
BsuI GTATCC 1 cut(s) 424
BsuRI GGCC 1 cut(s) 282
BsuTUI ATCGAT 1 cut(s) 408
BtsCI GGATG 5 cut(s) 86, 137, 376, 532, 712
BtsI GCAGTG 1 cut(s) 540
BtsIMutI CAGTG 4 cut(s) 144, 423, 540, 563
Cac8I GCNNGC 4 cut(s) 343, 686, 690, 768
CciI TCATGA 2 cut(s) 121, 205
Cfr13I GGNCC 1 cut(s) 187
ClaI ATCGAT 1 cut(s) 408
Csp6I GTAC 3 cut(s) 321, 391, 1020
CviAII CATG 7 cut(s) 122, 206, 342, 461, 524, 792, 819
CviQI GTAC 3 cut(s) 321, 391, 1020
DdeI CTNAG 1 cut(s) 804
DpnI GATC 3 cut(s) 387, 854, 898
DpnII GATC 3 cut(s) 385, 852, 896
Ecl136II GAGCTC 1 cut(s) 521
Eco130I CCWWGG 1 cut(s) 716
Eco24I GRGCYC 1 cut(s) 523
Eco31I GGTCTC 1 cut(s) 966
Eco32I GATATC 1 cut(s) 886
Eco47I GGWCC 1 cut(s) 187
Eco53kI GAGCTC 1 cut(s) 521
Eco88I CYCGRG 2 cut(s) 249, 573
EcoICRI GAGCTC 1 cut(s) 521
EcoRI GAATTC 1 cut(s) 215
EcoRII CCWGG 2 cut(s) 856, 879
EcoRV GATATC 1 cut(s) 886
EcoT14I CCWWGG 1 cut(s) 716
EcoT38I GRGCYC 1 cut(s) 523
ErhI CCWWGG 1 cut(s) 716
FaeI CATG 7 cut(s) 125, 209, 345, 464, 527, 795, 822
FalI AAGNNNNNCTT 2 cut(s) 897, 929
FaqI GGGAC 1 cut(s) 874
FatI CATG 7 cut(s) 121, 205, 341, 460, 523, 791, 818
FblI GTMKAC 1 cut(s) 375
Fnu4HI GCNGC 2 cut(s) 346, 780
FokI GGATG 5 cut(s) 93, 144, 383, 519, 699
FriOI GRGCYC 1 cut(s) 523
Fsp4HI GCNGC 2 cut(s) 346, 780
FspBI CTAG 3 cut(s) 66, 354, 498
GluI GCNGC 2 cut(s) 346, 780
GsuI CTGGAG 1 cut(s) 698
HaeIII GGCC 1 cut(s) 282
HapII CCGG 2 cut(s) 326, 607
Hin1II CATG 7 cut(s) 125, 209, 345, 464, 527, 795, 822
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HindIII AAGCTT 4 cut(s) 452, 611, 764, 911
HinfI GANTC 1 cut(s) 571
HpaII CCGG 2 cut(s) 326, 607
HphI GGTGA 1 cut(s) 209
Hpy166II GTNNAC 5 cut(s) 89, 309, 376, 570, 1022
Hpy188I TCNGA 4 cut(s) 187, 295, 493, 896
Hpy188III TCNNGA 9 cut(s) 66, 96, 122, 206, 251, 575, 677, 737, 850
Hpy8I GTNNAC 5 cut(s) 89, 309, 376, 570, 1022
HpyAV CCTTC 3 cut(s) 181, 458, 661
HpyCH4III ACNGT 2 cut(s) 404, 567
HpyCH4V TGCA 6 cut(s) 400, 460, 550, 692, 795, 1016
HpyF10VI GCNNNNNNNGC 2 cut(s) 451, 776
HpyF3I CTNAG 1 cut(s) 804
Hsp92II CATG 7 cut(s) 125, 209, 345, 464, 527, 795, 822
KpnI GGTACC 1 cut(s) 324
Kzo9I GATC 3 cut(s) 385, 852, 896
LmnI GCTCC 3 cut(s) 115, 442, 805
Lsp1109I GCAGC 2 cut(s) 332, 766
LweI GCATC 1 cut(s) 934
MaeI CTAG 3 cut(s) 66, 354, 498
MaeIII GTNAC 1 cut(s) 197
MalI GATC 3 cut(s) 387, 854, 898
MboI GATC 3 cut(s) 385, 852, 896
MfeI CAATTG 1 cut(s) 423
MhlI GDGCHC 1 cut(s) 523
MluCI AATT 6 cut(s) 215, 423, 594, 618, 936, 963
MlyI GAGTC 1 cut(s) 565
MmeI TCCRAC 3 cut(s) 286, 874, 882
MnlI CCTC 9 cut(s) 52, 123, 258, 357, 421, 651, 813, 916, 962
MroXI GAANNNNTTC 1 cut(s) 594
MseI TTAA 3 cut(s) 36, 447, 873
MslI CAYNNNNRTG 3 cut(s) 210, 512, 545
MspCI CTTAAG 2 cut(s) 446, 872
MspI CCGG 2 cut(s) 326, 607
MspR9I CCNGG 3 cut(s) 326, 858, 881
MunI CAATTG 1 cut(s) 423
Mva1269I GAATGC 1 cut(s) 787
MvaI CCWGG 2 cut(s) 858, 881
MwoI GCNNNNNNNGC 2 cut(s) 451, 776
NciI CCSGG 1 cut(s) 326
NdeII GATC 3 cut(s) 385, 852, 896
NlaIII CATG 7 cut(s) 125, 209, 345, 464, 527, 795, 822
NlaIV GGNNCC 1 cut(s) 322
NmuCI GTSAC 1 cut(s) 197
NspI RCATGY 1 cut(s) 345
OliI CACNNNNGTG 1 cut(s) 545
PaeI GCATGC 1 cut(s) 345
PaeR7I CTCGAG 2 cut(s) 249, 573
PagI TCATGA 2 cut(s) 121, 205
PasI CCCWGGG 1 cut(s) 857
PctI GAATGC 1 cut(s) 787
PdmI GAANNNNTTC 1 cut(s) 594
PkrI GCNGC 2 cut(s) 347, 781
PleI GAGTC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 565
Psp124BI GAGCTC 1 cut(s) 523
Psp6I CCWGG 2 cut(s) 856, 879
PspGI CCWGG 2 cut(s) 856, 879
PspN4I GGNNCC 1 cut(s) 322
PspPI GGNCC 1 cut(s) 187
PstI CTGCAG 1 cut(s) 694
RsaI GTAC 3 cut(s) 322, 392, 1021
RsaNI GTAC 3 cut(s) 321, 391, 1020
RseI CAYNNNNRTG 3 cut(s) 210, 512, 545
SacI GAGCTC 1 cut(s) 523
SalI GTCGAC 1 cut(s) 374
SaqAI TTAA 3 cut(s) 36, 447, 873
SatI GCNGC 2 cut(s) 346, 780
Sau3AI GATC 3 cut(s) 385, 852, 896
Sau96I GGNCC 1 cut(s) 187
SchI GAGTC 1 cut(s) 565
ScrFI CCNGG 3 cut(s) 326, 858, 881
SduI GDGCHC 1 cut(s) 523
SfaNI GCATC 1 cut(s) 934
SfcI CTRYAG 1 cut(s) 690
Sfr274I CTCGAG 2 cut(s) 249, 573
SinI GGWCC 1 cut(s) 187
SlaI CTCGAG 2 cut(s) 249, 573
SmiMI CAYNNNNRTG 3 cut(s) 210, 512, 545
SmlI CTYRAG 4 cut(s) 249, 446, 573, 872
SmoI CTYRAG 4 cut(s) 249, 446, 573, 872
SphI GCATGC 1 cut(s) 345
Sse9I AATT 6 cut(s) 215, 423, 594, 618, 936, 963
SspMI CTAG 3 cut(s) 66, 354, 498
SstI GAGCTC 1 cut(s) 523
StyD4I CCNGG 3 cut(s) 324, 856, 879
StyI CCWWGG 1 cut(s) 716
TaaI ACNGT 2 cut(s) 404, 567
TaqI TCGA 4 cut(s) 250, 375, 408, 574
TaqII GACCGA 2 cut(s) 635, 990
TasI AATT 6 cut(s) 215, 423, 594, 618, 936, 963
TatI WGTACW 2 cut(s) 390, 1019
Tru1I TTAA 3 cut(s) 36, 447, 873
Tru9I TTAA 3 cut(s) 36, 447, 873
TscAI CASTG 4 cut(s) 144, 423, 547, 570
TseFI GTSAC 1 cut(s) 197
TseI GCWGC 2 cut(s) 345, 779
Tsp45I GTSAC 1 cut(s) 197
TspRI CASTG 4 cut(s) 144, 423, 547, 570
Vha464I CTTAAG 2 cut(s) 446, 872
VpaK11BI GGWCC 1 cut(s) 187
XapI RAATTY 2 cut(s) 215, 936
XbaI TCTAGA 1 cut(s) 65
XceI RCATGY 1 cut(s) 345
XhoI CTCGAG 2 cut(s) 249, 573
XmiI GTMKAC 1 cut(s) 375
XmnI GAANNNNTTC 1 cut(s) 594
XspI CTAG 3 cut(s) 66, 354, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.