MD12G1239300.v1.1

aldo-keto reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000149
Physical Location & Seq
Reverse (-)
31206034 .. 31208133
2100 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1239300.v1.1.491

Sequence Viewer

Length: 639 bp
ATGGCAGAAGAGAACCAAGTCCAAGTTCAAAGAGTTAAACTCGGCAGCCAAGGACTTGAGGTCTCAAAGTTGGGGTTCGGATGCATGGGCCTTACCGGGACTTACAACTCTCCTGTCGCTGATGAGGATGGTATCGCTATAATAAAACATGCCTTCAACAAAGCAATCACTTTCTTTGACACAGCTGATGCGTATGGACCTCATACAAATGAAGTTCTTGTTGGAAAGGCCTTGAAGCAGCTGCCAAGGGAAAAAGTTCAACTGGCCACAAAGTTTGGTATTGTTGGAGTAGTGCCGCCTGCTGGTGTGGTAGTGAAGGGAACTCCTGAGTATGTCCGCTCATGCTGTGAGGCTAGCTTGAAACGTCTTGACGTGGACTACATTGATCTGTATTATTACCACCGGGTGGACACTTCGGTGCCTATAGAGGAAACTATGGATGAGCTGAAGAAACTGGTGGAAGAAGGAAAAATAAAGTACATTGGGTTATCTGAAGCCAGCCCTGACACAATAAGGAGAGCGCATGCAGTTCATCCGATCACGGCTCTGCAAATGGAGTGGTCCCTCTGGACTCGTGATATTGAGGAAGAGATTGTTCCACTTTTGTTGTTGAGTGTCATTGTAATACCTTTCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

213

Amino Acids

23.5

Weight (kDa)

5.29

Isoelectric Point (pI)

37.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 23 - 202 4.2e-51 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000149)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g01350 FvH4_4g01350 FvH4_4g01350 FvH4_4g01350 FvH4_4g01350 FvH4_4g01350 FvH4_4g01350 FvH4_4g01550 FvH4_4g01550 FvH4_4g01550 FvH4_6g03001 FvH4_6g03010 FvH4_6g03030 FvH4_6g03050 FvH4_6g03050 FvH4_6g03050 FvH4_6g03051 FvH4_6g03051 FvH4_6g03051 FvH4_6g03051 FvH4_6g03052 FvH4_6g03070 FvH4_6g03070 FvH4_6g03110
malus_domestica MD04G1223200.v1.1 MD08G1240600.v1.1 MD12G1239000.v1.1 MD12G1239100.v1.1 MD12G1239200.v1.1 MD12G1239300.v1.1 MD12G1239400.v1.1 MD12G1239600.v1.1 MD12G1239800.v1.1 MD12G1240000.v1.1 MD12G1240300.v1.1 MD12G1240400.v1.1 MD13G1200700.v1.1
prunus_persica Prupe.1G021900_v2.0.a1 Prupe.1G022000_v2.0.a1 Prupe.6G141300_v2.0.a1 Prupe.6G141300_v2.0.a1 Prupe.6G141300_v2.0.a1 Prupe.6G341900_v2.0.a1 Prupe.6G342100_v2.0.a1 Prupe.6G342200_v2.0.a1 Prupe.6G342300_v2.0.a1
pyrus_communis pycom04g19710 pycom12g21970 pycom12g21980 pycom12g21990 pycom12g22000 pycom12g22020 pycom13g17420 pycom16g16880
rosa_chinensis RchiOBHm_Chr3g0450451 RchiOBHm_Chr3g0451211 RchiOBHm_Chr3g0451221 RchiOBHm_Chr3g0451251 RchiOBHm_Chr3g0451261 RchiOBHm_Chr3g0451281 RchiOBHm_Chr3g0451321 RchiOBHm_Chr3g0451331 RchiOBHm_Chr3g0451341 RchiOBHm_Chr3g0454931 RchiOBHm_Chr4g0387811 RchiOBHm_Chr4g0387821 RchiOBHm_Chr4g0388101
rosa_laevigata RLG00000010080 RLG00000010092 RLG00000010093 RLG00000025402 RLG00000025666 RLG00000025667 RLG00000025668 RLG00000025669 RLG00000025672 RLG00000025673 RLG00000025674 RLG00000025675 RLG00000025676 RLG00000025678 RLG00000025679 RLG00000025729
rosa_multiflora Rmu_co8283913.1_g000001 Rmu_co8296899.1_g000001 Rmu_sc0000330.1_g000014 Rmu_sc0001792.1_g000004 Rmu_sc0002169.1_g000002 Rmu_sc0002180.1_g000028 Rmu_sc0002509.1_g000009 Rmu_sc0002575.1_g000011 Rmu_sc0002575.1_g000013 Rmu_sc0002575.1_g000015 Rmu_sc0002575.1_g000019 Rmu_sc0002575.1_g000021 Rmu_sc0002575.1_g000022 Rmu_sc0012229.1_g000010 Rmu_sc0012229.1_g000014 Rmu_sc0012229.1_g000017 Rmu_sc0012229.1_g000018 Rmu_sc0013958.1_g000001 Rmu_sc0030279.1_g000001 Rmu_sc0031850.1_g000002
rosa_roxburghii Rroxscaffold_163G00450840 Rroxscaffold_163G00450860 Rroxscaffold_163G00450870 Rroxscaffold_164G00436570 Rroxscaffold_5G00334430 Rroxscaffold_5G00334440 Rroxscaffold_5G00334650 Rroxscaffold_6G00428150 Rroxscaffold_6G00428660 Rroxscaffold_6G00428680 Rroxscaffold_6G00428710 Rroxscaffold_6G00428720 Rroxscaffold_6G00428730 Rroxscaffold_6G00428740 Rroxscaffold_6G00428750 Rroxscaffold_6G00428760
rosa_rugosa Rorug02G0535300 Rorug02G0627900 Rorug02G0632500 Rorug02G0632600 Rorug02G0633000 Rorug02G0633100 Rorug03G0003000 Rorug03G0313700 Rorug03G0314800 Rorug05G0229300 Rorug07G0155500
rosa_samantha Rh3AG028500 Rh3AG035300 Rh3AG035400 Rh3AG035500 Rh3AG035600 Rh3AG035700 Rh3AG035800 Rh3AG035900 Rh3AG062900 Rh3BG029000 Rh3BG035500 Rh3BG035600 Rh3BG035800 Rh3BG035900 Rh3BG036000 Rh3BG036100 Rh3BG036200 Rh3BG036300 Rh3BG036400 Rh3BG036500 Rh3BG036600 Rh3BG064800 Rh3CG027800 Rh3CG034200 Rh3CG034400 Rh3CG034500 Rh3CG034600 Rh3CG034700 Rh3CG034800 Rh3CG034900 Rh3CG063700 Rh3DG035100 Rh3DG035200 Rh3DG035400 Rh3DG035500 Rh3DG035600 Rh3DG035700 Rh3DG035800 Rh3DG035900 Rh3DG036000 Rh3DG036100 Rh3DG064800 Rh4AG018700 Rh4BG013600 Rh4BG013700 Rh4BG014900 Rh4CG021200 Rh4DG015600
rosa_wichuraiana Rw0G001850 Rw3G002200 Rw3G002580 Rw3G002590 Rw3G002610 Rw3G002620 Rw3G002630 Rw3G002640 Rw3G002650 Rw3G002660 Rw3G004920 Rw3G016120 Rw4G001220 Rw4G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 418
AccB7I CCANNNNNTGG 1 cut(s) 406
AccBSI CCGCTC 1 cut(s) 339
AciI CCGC 2 cut(s) 296, 337
AcoI YGGCCR 1 cut(s) 264
AcuI CTGAAG 2 cut(s) 467, 513
AdeI CACNNNGTG 1 cut(s) 406
AfaI GTAC 1 cut(s) 479
AfiI CCNNNNNNNGG 2 cut(s) 302, 406
AgsI TTSAA 5 cut(s) 29, 157, 235, 260, 361
AhdI GACNNNNNGTC 1 cut(s) 59
AjiI CACGTC 1 cut(s) 373
AjuI GAANNNNNNNTTGG 4 cut(s) 9, 41, 204, 236
AleI CACNNNNGTG 1 cut(s) 416
AluBI AGCT 4 cut(s) 185, 241, 357, 445
AluI AGCT 4 cut(s) 185, 241, 357, 445
Alw26I GTCTC 1 cut(s) 67
AoxI GGCC 3 cut(s) 88, 228, 264
ApeKI GCWGC 3 cut(s) 45, 238, 241
Asp700I GAANNNNTTC 1 cut(s) 255
AspLEI GCGC 1 cut(s) 523
AspS9I GGNCC 3 cut(s) 88, 197, 561
AsuC2I CCSGG 2 cut(s) 97, 404
AsuNHI GCTAGC 1 cut(s) 353
AvaII GGWCC 2 cut(s) 197, 561
BalI TGGCCA 1 cut(s) 266
BanI GGYRCC 1 cut(s) 418
BauI CACGAG 1 cut(s) 573
BbvI GCAGC 3 cut(s) 57, 228, 250
BccI CCATC 1 cut(s) 122
BceAI ACGGC 1 cut(s) 558
BcnI CCSGG 2 cut(s) 97, 404
BcoDI GTCTC 1 cut(s) 67
BfaI CTAG 1 cut(s) 354
BfmI CTRYAG 1 cut(s) 423
BisI GCNGC 4 cut(s) 46, 239, 242, 296
BlsI GCNGC 4 cut(s) 47, 240, 243, 297
Bme1390I CCNGG 2 cut(s) 97, 404
Bme18I GGWCC 2 cut(s) 197, 561
BmeRI GACNNNNNGTC 1 cut(s) 59
BmgBI CACGTC 1 cut(s) 373
BmgT120I GGNCC 3 cut(s) 88, 197, 561
BmiI GGNNCC 2 cut(s) 420, 563
BmrFI CCNGG 2 cut(s) 97, 404
BmsI GCATC 2 cut(s) 71, 178
BmtI GCTAGC 1 cut(s) 357
BplI GAGNNNNNCTC 2 cut(s) 24, 56
BpuEI CTTGAG 1 cut(s) 77
BpuMI CCSGG 2 cut(s) 97, 404
BsaI GGTCTC 1 cut(s) 67
BsaJI CCNNGG 2 cut(s) 49, 245
Bsc4I CCNNNNNNNGG 2 cut(s) 302, 406
Bse1I ACTGG 2 cut(s) 267, 459
BseDI CCNNGG 2 cut(s) 49, 245
BseGI GGATG 4 cut(s) 86, 133, 445, 532
BseLI CCNNNNNNNGG 2 cut(s) 302, 406
BseMII CTCAG 1 cut(s) 318
BseNI ACTGG 2 cut(s) 267, 459
BseXI GCAGC 3 cut(s) 57, 228, 250
BshFI GGCC 3 cut(s) 90, 230, 266
BshNI GGYRCC 1 cut(s) 418
BsiSI CCGG 2 cut(s) 96, 403
BslFI GGGAC 2 cut(s) 112, 547
BslI CCNNNNNNNGG 2 cut(s) 302, 406
BsmAI GTCTC 1 cut(s) 67
BsmFI GGGAC 2 cut(s) 112, 547
BsnI GGCC 3 cut(s) 90, 230, 266
Bso31I GGTCTC 1 cut(s) 67
Bsp143I GATC 2 cut(s) 385, 537
BspACI CCGC 2 cut(s) 296, 337
BspANI GGCC 3 cut(s) 90, 230, 266
BspCNI CTCAG 1 cut(s) 319
BspLI GGNNCC 2 cut(s) 420, 563
BspOI GCTAGC 1 cut(s) 357
BspT107I GGYRCC 1 cut(s) 418
BspTNI GGTCTC 1 cut(s) 67
BsrBI CCGCTC 1 cut(s) 339
BsrI ACTGG 2 cut(s) 267, 459
BssECI CCNNGG 2 cut(s) 49, 245
BssMI GATC 2 cut(s) 385, 537
BssSI CACGAG 1 cut(s) 573
BssT1I CCWWGG 2 cut(s) 49, 245
Bst2BI CACGAG 1 cut(s) 573
Bst6I CTCTTC 2 cut(s) 3, 582
BstC8I GCNNGC 4 cut(s) 300, 355, 499, 525
BstDEI CTNAG 1 cut(s) 327
BstF5I GGATG 4 cut(s) 86, 133, 445, 532
BstHHI GCGC 1 cut(s) 523
BstKTI GATC 2 cut(s) 388, 540
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 2 cut(s) 385, 537
BstNSI RCATGY 2 cut(s) 152, 527
BstSCI CCNGG 2 cut(s) 95, 402
BstSFI CTRYAG 1 cut(s) 423
BstV1I GCAGC 3 cut(s) 57, 228, 250
BsuRI GGCC 3 cut(s) 90, 230, 266
BtrI CACGTC 1 cut(s) 373
BtsCI GGATG 4 cut(s) 86, 133, 445, 532
Cac8I GCNNGC 4 cut(s) 300, 355, 499, 525
CfoI GCGC 1 cut(s) 523
Cfr13I GGNCC 3 cut(s) 88, 197, 561
Csp6I GTAC 1 cut(s) 478
CspCI CAANNNNNGTGG 6 cut(s) 256, 291, 539, 574, 588, 623
CviAII CATG 4 cut(s) 85, 149, 342, 524
CviQI GTAC 1 cut(s) 478
DdeI CTNAG 1 cut(s) 327
DpnI GATC 2 cut(s) 387, 539
DpnII GATC 2 cut(s) 385, 537
DraIII CACNNNGTG 1 cut(s) 406
DriI GACNNNNNGTC 1 cut(s) 59
EaeI YGGCCR 1 cut(s) 264
Eam1104I CTCTTC 2 cut(s) 3, 582
Eam1105I GACNNNNNGTC 1 cut(s) 59
EarI CTCTTC 2 cut(s) 3, 582
Eco130I CCWWGG 2 cut(s) 49, 245
Eco147I AGGCCT 1 cut(s) 230
Eco31I GGTCTC 1 cut(s) 67
Eco47I GGWCC 2 cut(s) 197, 561
Eco57I CTGAAG 2 cut(s) 467, 513
EcoT14I CCWWGG 2 cut(s) 49, 245
EcoT22I ATGCAT 1 cut(s) 86
ErhI CCWWGG 2 cut(s) 49, 245
FaeI CATG 4 cut(s) 88, 152, 345, 527
FaqI GGGAC 2 cut(s) 112, 547
FatI CATG 4 cut(s) 84, 148, 341, 523
Fnu4HI GCNGC 4 cut(s) 46, 239, 242, 296
FokI GGATG 4 cut(s) 93, 140, 452, 519
Fsp4HI GCNGC 4 cut(s) 46, 239, 242, 296
FspBI CTAG 1 cut(s) 354
GlaI GCGC 1 cut(s) 522
GluI GCNGC 4 cut(s) 46, 239, 242, 296
HaeIII GGCC 3 cut(s) 90, 230, 266
HapII CCGG 2 cut(s) 96, 403
HhaI GCGC 1 cut(s) 523
Hin1II CATG 4 cut(s) 88, 152, 345, 527
Hin6I GCGC 1 cut(s) 521
HinP1I GCGC 1 cut(s) 521
HinfI GANTC 1 cut(s) 571
HpaII CCGG 2 cut(s) 96, 403
Hpy166II GTNNAC 2 cut(s) 376, 409
Hpy188I TCNGA 3 cut(s) 80, 493, 537
Hpy188III TCNNGA 4 cut(s) 326, 368, 568, 575
Hpy8I GTNNAC 2 cut(s) 376, 409
HpyAV CCTTC 3 cut(s) 163, 310, 458
HpyCH4IV ACGT 2 cut(s) 364, 372
HpyCH4V TGCA 3 cut(s) 84, 527, 550
HpyF3I CTNAG 1 cut(s) 327
HpySE526I ACGT 2 cut(s) 364, 372
Hsp92II CATG 4 cut(s) 88, 152, 345, 527
HspAI GCGC 1 cut(s) 521
Kzo9I GATC 2 cut(s) 385, 537
Lsp1109I GCAGC 3 cut(s) 57, 228, 250
LweI GCATC 2 cut(s) 71, 178
MaeI CTAG 1 cut(s) 354
MaeII ACGT 2 cut(s) 364, 372
MalI GATC 2 cut(s) 387, 539
MbiI CCGCTC 1 cut(s) 339
MboI GATC 2 cut(s) 385, 537
MboII GAAGA 4 cut(s) 20, 460, 473, 599
MlsI TGGCCA 1 cut(s) 266
MluNI TGGCCA 1 cut(s) 266
MlyI GAGTC 1 cut(s) 565
MmeI TCCRAC 2 cut(s) 202, 265
MnlI CCTC 7 cut(s) 52, 118, 210, 343, 421, 575, 577
Mox20I TGGCCA 1 cut(s) 266
Mph1103I ATGCAT 1 cut(s) 86
MroXI GAANNNNTTC 1 cut(s) 255
MscI TGGCCA 1 cut(s) 266
MseI TTAA 1 cut(s) 36
MslI CAYNNNNRTG 2 cut(s) 207, 416
Msp20I TGGCCA 1 cut(s) 266
MspA1I CMGCKG 2 cut(s) 185, 241
MspI CCGG 2 cut(s) 96, 403
MspR9I CCNGG 2 cut(s) 97, 404
NciI CCSGG 2 cut(s) 97, 404
NdeII GATC 2 cut(s) 385, 537
NheI GCTAGC 1 cut(s) 353
NlaIII CATG 4 cut(s) 88, 152, 345, 527
NlaIV GGNNCC 2 cut(s) 420, 563
NmeAIII GCCGAG 1 cut(s) 21
NsiI ATGCAT 1 cut(s) 86
NspI RCATGY 2 cut(s) 152, 527
OliI CACNNNNGTG 1 cut(s) 416
PaeI GCATGC 1 cut(s) 527
PceI AGGCCT 1 cut(s) 230
PdmI GAANNNNTTC 1 cut(s) 255
PflMI CCANNNNNTGG 1 cut(s) 406
PkrI GCNGC 4 cut(s) 47, 240, 243, 297
PleI GAGTC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 565
PspN4I GGNNCC 2 cut(s) 420, 563
PspPI GGNCC 3 cut(s) 88, 197, 561
PvuII CAGCTG 2 cut(s) 185, 241
RsaI GTAC 1 cut(s) 479
RsaNI GTAC 1 cut(s) 478
RseI CAYNNNNRTG 2 cut(s) 207, 416
SaqAI TTAA 1 cut(s) 36
SatI GCNGC 4 cut(s) 46, 239, 242, 296
Sau3AI GATC 2 cut(s) 385, 537
Sau96I GGNCC 3 cut(s) 88, 197, 561
SchI GAGTC 1 cut(s) 565
ScrFI CCNGG 2 cut(s) 97, 404
SetI ASST 9 cut(s) 63, 187, 202, 243, 359, 367, 375, 447, 631
SfaNI GCATC 2 cut(s) 71, 178
SfcI CTRYAG 1 cut(s) 423
SinI GGWCC 2 cut(s) 197, 561
SmiMI CAYNNNNRTG 2 cut(s) 207, 416
SmlI CTYRAG 1 cut(s) 56
SmoI CTYRAG 1 cut(s) 56
SphI GCATGC 1 cut(s) 527
SseBI AGGCCT 1 cut(s) 230
SsiI CCGC 2 cut(s) 296, 337
SspMI CTAG 1 cut(s) 354
StuI AGGCCT 1 cut(s) 230
StyD4I CCNGG 2 cut(s) 95, 402
StyI CCWWGG 2 cut(s) 49, 245
TaiI ACGT 2 cut(s) 367, 375
TatI WGTACW 1 cut(s) 477
TauI GCSGC 1 cut(s) 298
Tru1I TTAA 1 cut(s) 36
Tru9I TTAA 1 cut(s) 36
TseI GCWGC 3 cut(s) 45, 238, 241
TspDTI ATGAA 2 cut(s) 225, 521
Van91I CCANNNNNTGG 1 cut(s) 406
VpaK11BI GGWCC 2 cut(s) 197, 561
XceI RCATGY 2 cut(s) 152, 527
XmnI GAANNNNTTC 1 cut(s) 255
XspI CTAG 1 cut(s) 354
Zsp2I ATGCAT 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.