Rmu_sc0013958.1_g000001

Perakine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0013958.1
OG0000149
Physical Location & Seq
Forward (+)
1331 .. 3454
2124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0013958.1_g000001.1.cds

Sequence Viewer

Length: 1035 bp
atggaggacaaatctgatattgaaatcccaaaggttaaactgggcagccaaggcttggaggtgtctagattgggttttggatgtgggggactatctggaatatacaatgctcctctctctcatgaagctggatgttcagtgatacaggaagcattcaataggggcatcaccttctacgatactgcagatgcttatggtcaggatcatgataatgaaatcatggtcggcaaggctttaaaacaacttcctcgagaaaaggttcaattggctacaaaatttggcattctcgagtcagacagctttgaattcagtgtaaatgggaccccggagtatgtaagagcatgctgtgaagctagtctgaagaggctggatgtcgactacattgatttgtactatcagcaccgtgttgatgtttcagtgccaattgaggatacgattgaggagcttaagaagctagtgaatgaaggaaaaataagatacattggcctttcagaagctagtatagacacaataatgagagctcatgctgttcatcccattactgcagtgcaaatggagtactctctttggactcgtgaaattgaaaatgagataattccactttgcaggaaacttggaattgggatagtatcatatggtccacttggtcgtggcttcttcggtgggaaggcaattatagagagcttgcctgccgaaagtttgttgtctattcatccaaggttcaatggtgagaatttggagaaaaacaaacttttctataacaaacttgccaaccttgctgcaaaacattcatgcactgctcctcagctagctctggcatggcttctccatcagggcaatgacatagttccaatcccaggaactactaaagttaagaacctttgtatcaatattggagcattgaatgtgaagcttacgaaagaggatttgactgaaatttgtgatgctgttcccaataatgaagttgaaggcgaccgagaatttgaagttctgtcaaaattcatgtggaataacgcaaataccccatcaaagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

344

Amino Acids

38.12

Weight (kDa)

5.32

Isoelectric Point (pI)

33.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000149)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g01350 FvH4_4g01350 FvH4_4g01350 FvH4_4g01350 FvH4_4g01350 FvH4_4g01350 FvH4_4g01350 FvH4_4g01550 FvH4_4g01550 FvH4_4g01550 FvH4_6g03001 FvH4_6g03010 FvH4_6g03030 FvH4_6g03050 FvH4_6g03050 FvH4_6g03050 FvH4_6g03051 FvH4_6g03051 FvH4_6g03051 FvH4_6g03051 FvH4_6g03052 FvH4_6g03070 FvH4_6g03070 FvH4_6g03110
malus_domestica MD04G1223200.v1.1 MD08G1240600.v1.1 MD12G1239000.v1.1 MD12G1239100.v1.1 MD12G1239200.v1.1 MD12G1239300.v1.1 MD12G1239400.v1.1 MD12G1239600.v1.1 MD12G1239800.v1.1 MD12G1240000.v1.1 MD12G1240300.v1.1 MD12G1240400.v1.1 MD13G1200700.v1.1
prunus_persica Prupe.1G021900_v2.0.a1 Prupe.1G022000_v2.0.a1 Prupe.6G141300_v2.0.a1 Prupe.6G141300_v2.0.a1 Prupe.6G141300_v2.0.a1 Prupe.6G341900_v2.0.a1 Prupe.6G342100_v2.0.a1 Prupe.6G342200_v2.0.a1 Prupe.6G342300_v2.0.a1
pyrus_communis pycom04g19710 pycom12g21970 pycom12g21980 pycom12g21990 pycom12g22000 pycom12g22020 pycom13g17420 pycom16g16880
rosa_chinensis RchiOBHm_Chr3g0450451 RchiOBHm_Chr3g0451211 RchiOBHm_Chr3g0451221 RchiOBHm_Chr3g0451251 RchiOBHm_Chr3g0451261 RchiOBHm_Chr3g0451281 RchiOBHm_Chr3g0451321 RchiOBHm_Chr3g0451331 RchiOBHm_Chr3g0451341 RchiOBHm_Chr3g0454931 RchiOBHm_Chr4g0387811 RchiOBHm_Chr4g0387821 RchiOBHm_Chr4g0388101
rosa_laevigata RLG00000010080 RLG00000010092 RLG00000010093 RLG00000025402 RLG00000025666 RLG00000025667 RLG00000025668 RLG00000025669 RLG00000025672 RLG00000025673 RLG00000025674 RLG00000025675 RLG00000025676 RLG00000025678 RLG00000025679 RLG00000025729
rosa_multiflora Rmu_co8283913.1_g000001 Rmu_co8296899.1_g000001 Rmu_sc0000330.1_g000014 Rmu_sc0001792.1_g000004 Rmu_sc0002169.1_g000002 Rmu_sc0002180.1_g000028 Rmu_sc0002509.1_g000009 Rmu_sc0002575.1_g000011 Rmu_sc0002575.1_g000013 Rmu_sc0002575.1_g000015 Rmu_sc0002575.1_g000019 Rmu_sc0002575.1_g000021 Rmu_sc0002575.1_g000022 Rmu_sc0012229.1_g000010 Rmu_sc0012229.1_g000014 Rmu_sc0012229.1_g000017 Rmu_sc0012229.1_g000018 Rmu_sc0013958.1_g000001 Rmu_sc0030279.1_g000001 Rmu_sc0031850.1_g000002
rosa_roxburghii Rroxscaffold_163G00450840 Rroxscaffold_163G00450860 Rroxscaffold_163G00450870 Rroxscaffold_164G00436570 Rroxscaffold_5G00334430 Rroxscaffold_5G00334440 Rroxscaffold_5G00334650 Rroxscaffold_6G00428150 Rroxscaffold_6G00428660 Rroxscaffold_6G00428680 Rroxscaffold_6G00428710 Rroxscaffold_6G00428720 Rroxscaffold_6G00428730 Rroxscaffold_6G00428740 Rroxscaffold_6G00428750 Rroxscaffold_6G00428760
rosa_rugosa Rorug02G0535300 Rorug02G0627900 Rorug02G0632500 Rorug02G0632600 Rorug02G0633000 Rorug02G0633100 Rorug03G0003000 Rorug03G0313700 Rorug03G0314800 Rorug05G0229300 Rorug07G0155500
rosa_samantha Rh3AG028500 Rh3AG035300 Rh3AG035400 Rh3AG035500 Rh3AG035600 Rh3AG035700 Rh3AG035800 Rh3AG035900 Rh3AG062900 Rh3BG029000 Rh3BG035500 Rh3BG035600 Rh3BG035800 Rh3BG035900 Rh3BG036000 Rh3BG036100 Rh3BG036200 Rh3BG036300 Rh3BG036400 Rh3BG036500 Rh3BG036600 Rh3BG064800 Rh3CG027800 Rh3CG034200 Rh3CG034400 Rh3CG034500 Rh3CG034600 Rh3CG034700 Rh3CG034800 Rh3CG034900 Rh3CG063700 Rh3DG035100 Rh3DG035200 Rh3DG035400 Rh3DG035500 Rh3DG035600 Rh3DG035700 Rh3DG035800 Rh3DG035900 Rh3DG036000 Rh3DG036100 Rh3DG064800 Rh4AG018700 Rh4BG013600 Rh4BG013700 Rh4BG014900 Rh4CG021200 Rh4DG015600
rosa_wichuraiana Rw0G001850 Rw3G002200 Rw3G002580 Rw3G002590 Rw3G002610 Rw3G002620 Rw3G002630 Rw3G002640 Rw3G002650 Rw3G002660 Rw3G004920 Rw3G016120 Rw4G001220 Rw4G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 55
AccI GTMKAC 1 cut(s) 375
AclWI GGATC 1 cut(s) 210
AcsI RAATTY 6 cut(s) 275, 305, 733, 936, 980, 998
AcuI CTGAAG 1 cut(s) 380
AfaI GTAC 2 cut(s) 392, 560
AfiI CCNNNNNNNGG 2 cut(s) 55, 857
AflII CTTAAG 1 cut(s) 446
AgsI TTSAA 9 cut(s) 23, 157, 263, 305, 584, 724, 904, 968, 986
AjnI CCWGG 1 cut(s) 856
Alw21I GWGCWC 1 cut(s) 523
AlwI GGATC 1 cut(s) 210
Ama87I CYCGRG 2 cut(s) 249, 287
AoxI GGCC 1 cut(s) 484
ApeKI GCWGC 2 cut(s) 45, 779
ApoI RAATTY 6 cut(s) 275, 305, 733, 936, 980, 998
ArsI GACNNNNNNTTYG 2 cut(s) 965, 997
Asp700I GAANNNNTTC 1 cut(s) 258
AspS9I GGNCC 2 cut(s) 321, 638
AsuC2I CCSGG 1 cut(s) 326
AsuHPI GGTGA 2 cut(s) 160, 740
AsuNHI GCTAGC 1 cut(s) 808
AvaI CYCGRG 2 cut(s) 249, 287
AvaII GGWCC 2 cut(s) 321, 638
BaeI ACNNNNGTAYC 2 cut(s) 868, 901
BanII GRGCYC 1 cut(s) 523
BauI CACGAG 1 cut(s) 573
Bbv12I GWGCWC 1 cut(s) 523
BbvCI CCTCAGC 1 cut(s) 804
BbvI GCAGC 2 cut(s) 57, 766
BccI CCATC 2 cut(s) 837, 1033
BciT130I CCWGG 1 cut(s) 858
BciVI GTATCC 1 cut(s) 424
BcnI CCSGG 1 cut(s) 326
BfaI CTAG 5 cut(s) 66, 354, 455, 498, 809
BfmI CTRYAG 2 cut(s) 183, 543
BfrI CTTAAG 1 cut(s) 446
BfuI GTATCC 1 cut(s) 424
BisI GCNGC 2 cut(s) 46, 780
BlsI GCNGC 2 cut(s) 47, 781
BmcAI AGTACT 1 cut(s) 560
Bme1390I CCNGG 2 cut(s) 326, 858
Bme18I GGWCC 2 cut(s) 321, 638
BmeT110I CYCGRG 2 cut(s) 249, 287
BmgT120I GGNCC 2 cut(s) 321, 638
BmiI GGNNCC 2 cut(s) 322, 323
BmrFI CCNGG 2 cut(s) 326, 858
BmrI ACTGGG 1 cut(s) 50
BmsI GCATC 3 cut(s) 174, 178, 934
BmtI GCTAGC 1 cut(s) 812
BmuI ACTGGG 1 cut(s) 50
Bpu10I CCTNAGC 1 cut(s) 804
BpuMI CCSGG 1 cut(s) 326
BsaJI CCNNGG 4 cut(s) 49, 324, 716, 856
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 857
Bse1I ACTGG 1 cut(s) 45
Bse3DI GCAATG 1 cut(s) 844
BseBI CCWGG 1 cut(s) 858
BseDI CCNNGG 4 cut(s) 49, 324, 716, 856
BseGI GGATG 5 cut(s) 86, 137, 376, 532, 712
BseLI CCNNNNNNNGG 2 cut(s) 55, 857
BseMI GCAATG 1 cut(s) 844
BseMII CTCAG 1 cut(s) 818
BseNI ACTGG 1 cut(s) 45
BseRI GAGGAG 3 cut(s) 102, 455, 792
BseXI GCAGC 2 cut(s) 57, 766
Bsh1285I CGRYCG 1 cut(s) 976
BshFI GGCC 1 cut(s) 486
BsiEI CGRYCG 1 cut(s) 976
BsiHKAI GWGCWC 1 cut(s) 523
BsiHKCI CYCGRG 2 cut(s) 249, 287
BsiSI CCGG 1 cut(s) 326
BslFI GGGAC 2 cut(s) 102, 334
BslI CCNNNNNNNGG 2 cut(s) 55, 857
BsmFI GGGAC 2 cut(s) 102, 334
BsmI GAATGC 2 cut(s) 152, 282
BsnI GGCC 1 cut(s) 486
BsoBI CYCGRG 2 cut(s) 249, 287
Bsp1286I GDGCHC 1 cut(s) 523
Bsp143I GATC 1 cut(s) 202
BspANI GGCC 1 cut(s) 486
BspCNI CTCAG 1 cut(s) 817
BspHI TCATGA 2 cut(s) 121, 205
BspLI GGNNCC 2 cut(s) 322, 323
BspMAI CTGCAG 2 cut(s) 187, 547
BspOI GCTAGC 1 cut(s) 812
BspPI GGATC 1 cut(s) 210
BspTI CTTAAG 1 cut(s) 446
BsrDI GCAATG 1 cut(s) 844
BsrI ACTGG 1 cut(s) 45
BssECI CCNNGG 4 cut(s) 49, 324, 716, 856
BssMI GATC 1 cut(s) 202
BssSI CACGAG 1 cut(s) 573
BssT1I CCWWGG 2 cut(s) 49, 716
Bst2BI CACGAG 1 cut(s) 573
Bst2UI CCWGG 1 cut(s) 858
Bst4CI ACNGT 1 cut(s) 404
Bst6I CTCTTC 1 cut(s) 356
BstAFI CTTAAG 1 cut(s) 446
BstC8I GCNNGC 4 cut(s) 343, 686, 690, 810
BstDEI CTNAG 1 cut(s) 804
BstF5I GGATG 5 cut(s) 86, 137, 376, 532, 712
BstKTI GATC 1 cut(s) 205
BstMBI GATC 1 cut(s) 202
BstMCI CGRYCG 1 cut(s) 976
BstMWI GCNNNNNNNGC 3 cut(s) 51, 451, 776
BstNI CCWGG 1 cut(s) 858
BstNSI RCATGY 1 cut(s) 345
BstSCI CCNGG 2 cut(s) 324, 856
BstSFI CTRYAG 2 cut(s) 183, 543
BstV1I GCAGC 2 cut(s) 57, 766
BsuI GTATCC 1 cut(s) 424
BsuRI GGCC 1 cut(s) 486
BtsCI GGATG 5 cut(s) 86, 137, 376, 532, 712
BtsI GCAGTG 2 cut(s) 552, 795
BtsIMutI CAGTG 5 cut(s) 144, 316, 423, 552, 795
Cac8I GCNNGC 4 cut(s) 343, 686, 690, 810
CciI TCATGA 2 cut(s) 121, 205
Cfr13I GGNCC 2 cut(s) 321, 638
Csp6I GTAC 2 cut(s) 391, 559
CviAII CATG 8 cut(s) 122, 206, 220, 342, 524, 792, 819, 1003
CviQI GTAC 2 cut(s) 391, 559
DdeI CTNAG 1 cut(s) 804
DpnI GATC 1 cut(s) 204
DpnII GATC 1 cut(s) 202
DraI TTTAAA 1 cut(s) 237
Eam1104I CTCTTC 1 cut(s) 356
EarI CTCTTC 1 cut(s) 356
Ecl136II GAGCTC 1 cut(s) 521
Eco130I CCWWGG 2 cut(s) 49, 716
Eco24I GRGCYC 1 cut(s) 523
Eco47I GGWCC 2 cut(s) 321, 638
Eco53kI GAGCTC 1 cut(s) 521
Eco57I CTGAAG 1 cut(s) 380
Eco88I CYCGRG 2 cut(s) 249, 287
EcoICRI GAGCTC 1 cut(s) 521
EcoO109I RGGNCCY 1 cut(s) 321
EcoRI GAATTC 1 cut(s) 305
EcoRII CCWGG 1 cut(s) 856
EcoT14I CCWWGG 2 cut(s) 49, 716
EcoT38I GRGCYC 1 cut(s) 523
ErhI CCWWGG 2 cut(s) 49, 716
FaeI CATG 8 cut(s) 125, 209, 223, 345, 527, 795, 822, 1006
FaqI GGGAC 2 cut(s) 102, 334
FatI CATG 8 cut(s) 121, 205, 219, 341, 523, 791, 818, 1002
FauNDI CATATG 1 cut(s) 634
FblI GTMKAC 1 cut(s) 375
Fnu4HI GCNGC 2 cut(s) 46, 780
FokI GGATG 5 cut(s) 93, 144, 383, 519, 699
FriOI GRGCYC 1 cut(s) 523
Fsp4HI GCNGC 2 cut(s) 46, 780
FspBI CTAG 5 cut(s) 66, 354, 455, 498, 809
GluI GCNGC 2 cut(s) 46, 780
HaeIII GGCC 1 cut(s) 486
HapII CCGG 1 cut(s) 326
Hin1II CATG 8 cut(s) 125, 209, 223, 345, 527, 795, 822, 1006
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HindIII AAGCTT 1 cut(s) 911
HinfI GANTC 2 cut(s) 290, 571
HpaII CCGG 1 cut(s) 326
HphI GGTGA 2 cut(s) 160, 740
Hpy166II GTNNAC 2 cut(s) 376, 641
Hpy188I TCNGA 4 cut(s) 16, 295, 360, 493
Hpy188III TCNNGA 8 cut(s) 66, 96, 122, 200, 206, 251, 287, 575
Hpy8I GTNNAC 2 cut(s) 376, 641
HpyAV CCTTC 4 cut(s) 181, 458, 661, 962
HpyCH4III ACNGT 1 cut(s) 404
HpyCH4V TGCA 6 cut(s) 185, 545, 550, 606, 782, 795
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 451, 776
HpyF3I CTNAG 1 cut(s) 804
Hsp92II CATG 8 cut(s) 125, 209, 223, 345, 527, 795, 822, 1006
KflI GGGWCCC 1 cut(s) 321
Kzo9I GATC 1 cut(s) 202
LmnI GCTCC 4 cut(s) 115, 442, 805, 896
Lsp1109I GCAGC 2 cut(s) 57, 766
LweI GCATC 3 cut(s) 174, 178, 934
MaeI CTAG 5 cut(s) 66, 354, 455, 498, 809
MalI GATC 1 cut(s) 204
MboI GATC 1 cut(s) 202
MboII GAAGA 2 cut(s) 373, 649
MfeI CAATTG 2 cut(s) 263, 423
MhlI GDGCHC 1 cut(s) 523
MlyI GAGTC 2 cut(s) 299, 565
MnlI CCTC 8 cut(s) 52, 123, 258, 357, 421, 433, 813, 916
MroXI GAANNNNTTC 1 cut(s) 258
MseI TTAA 4 cut(s) 36, 236, 447, 873
MslI CAYNNNNRTG 2 cut(s) 210, 512
MspCI CTTAAG 1 cut(s) 446
MspI CCGG 1 cut(s) 326
MspR9I CCNGG 2 cut(s) 326, 858
MunI CAATTG 2 cut(s) 263, 423
Mva1269I GAATGC 2 cut(s) 152, 282
MvaI CCWGG 1 cut(s) 858
MwoI GCNNNNNNNGC 3 cut(s) 51, 451, 776
NciI CCSGG 1 cut(s) 326
NdeI CATATG 1 cut(s) 634
NdeII GATC 1 cut(s) 202
NheI GCTAGC 1 cut(s) 808
NlaIII CATG 8 cut(s) 125, 209, 223, 345, 527, 795, 822, 1006
NlaIV GGNNCC 2 cut(s) 322, 323
NspI RCATGY 1 cut(s) 345
PaeI GCATGC 1 cut(s) 345
PaeR7I CTCGAG 2 cut(s) 249, 287
PagI TCATGA 2 cut(s) 121, 205
PctI GAATGC 2 cut(s) 152, 282
PdmI GAANNNNTTC 1 cut(s) 258
PflMI CCANNNNNTGG 1 cut(s) 55
PkrI GCNGC 2 cut(s) 47, 781
PleI GAGTC 2 cut(s) 298, 565
PpsI GAGTC 2 cut(s) 298, 565
PpuMI RGGWCCY 1 cut(s) 321
Psp124BI GAGCTC 1 cut(s) 523
Psp5II RGGWCCY 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 856
PspGI CCWGG 1 cut(s) 856
PspN4I GGNNCC 2 cut(s) 322, 323
PspPI GGNCC 2 cut(s) 321, 638
PspPPI RGGWCCY 1 cut(s) 321
PstI CTGCAG 2 cut(s) 187, 547
RsaI GTAC 2 cut(s) 392, 560
RsaNI GTAC 2 cut(s) 391, 559
RseI CAYNNNNRTG 2 cut(s) 210, 512
SacI GAGCTC 1 cut(s) 523
SalI GTCGAC 1 cut(s) 374
SaqAI TTAA 4 cut(s) 36, 236, 447, 873
SatI GCNGC 2 cut(s) 46, 780
Sau3AI GATC 1 cut(s) 202
Sau96I GGNCC 2 cut(s) 321, 638
ScaI AGTACT 1 cut(s) 560
SchI GAGTC 2 cut(s) 299, 565
ScrFI CCNGG 2 cut(s) 326, 858
SduI GDGCHC 1 cut(s) 523
SfaNI GCATC 3 cut(s) 174, 178, 934
SfcI CTRYAG 2 cut(s) 183, 543
Sfr274I CTCGAG 2 cut(s) 249, 287
SinI GGWCC 2 cut(s) 321, 638
SlaI CTCGAG 2 cut(s) 249, 287
SmiMI CAYNNNNRTG 2 cut(s) 210, 512
SmlI CTYRAG 3 cut(s) 249, 287, 446
SmoI CTYRAG 3 cut(s) 249, 287, 446
SphI GCATGC 1 cut(s) 345
SspI AATATT 1 cut(s) 892
SspMI CTAG 5 cut(s) 66, 354, 455, 498, 809
SstI GAGCTC 1 cut(s) 523
StyD4I CCNGG 2 cut(s) 324, 856
StyI CCWWGG 2 cut(s) 49, 716
TaaI ACNGT 1 cut(s) 404
TaqI TCGA 3 cut(s) 250, 288, 375
TaqII GACCGA 1 cut(s) 990
TatI WGTACW 2 cut(s) 390, 558
Tru1I TTAA 4 cut(s) 36, 236, 447, 873
Tru9I TTAA 4 cut(s) 36, 236, 447, 873
TscAI CASTG 5 cut(s) 144, 316, 423, 552, 802
TseI GCWGC 2 cut(s) 45, 779
TspDTI ATGAA 8 cut(s) 138, 228, 477, 521, 701, 780, 975, 991
TspRI CASTG 5 cut(s) 144, 316, 423, 552, 802
Van91I CCANNNNNTGG 1 cut(s) 55
Vha464I CTTAAG 1 cut(s) 446
VpaK11BI GGWCC 2 cut(s) 321, 638
XapI RAATTY 6 cut(s) 275, 305, 733, 936, 980, 998
XbaI TCTAGA 1 cut(s) 65
XceI RCATGY 1 cut(s) 345
XhoI CTCGAG 2 cut(s) 249, 287
XmiI GTMKAC 1 cut(s) 375
XmnI GAANNNNTTC 1 cut(s) 258
XspI CTAG 5 cut(s) 66, 354, 455, 498, 809
ZrmI AGTACT 1 cut(s) 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.