Rroxscaffold_5G00334650

Perakine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000149
Physical Location & Seq
Forward (+)
1910085 .. 1912086
2002 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00334650.1

Sequence Viewer

Length: 1038 bp
ATGGAGGACAACTACCAGATTCAAATCCCAAAGGTTCAACTGGGTAGTCAGGGATTAGAGGTTTCTAGATTGGGTTTTGGATGTGGTGGACTATCTGGAATATACAAAGCTCCTCCTCTCTCTCATGAAGCTGGGTGTTCTGTGATAAAGGAAGCATTTGATAGGGGCATCACCTTCTTTGACACTTCAGATCTTTATGGTCATAATCATGATAATGAAATCATGGTTGGGAAGGCTTTGAAACAGTTTCCTCGAGAAAAGGTTCAATTGGCTACAAAATTCGGCATTTTCAAGTCGGATGACTTTCAACTCGGGATAAATGGGAACCCGGAATATGTAAGAGCATGCTGTGAAGCTAGTCTAAAGCGGCTAGATGTTGACTACATTGATCTGTACTACCAGCACCGTGTCGATGTTTCAGTGGCAATTGAGGATACTATGGGGGAGCTTAAGAAGTTGGTAAATGAAGGAAAGATAATGTACATTGGTCTATCAGAAGCGAGTATAGACACAATAATGAGGGCTCATGCTGTTCATCCCATCACTGCTGTGCAAATGGAATATTCTCTTTGGACTCGTGATATTGAAGATGAGATTATTCCACTTTGCCGAAAGCTTGGAATTGGGATAGTGTCATATAGTCCCCTCGGTCGTGGGTTCTTCGGTGGGAAGGCAGTTCTAGAGAGCTTGCCTGCAGAAAGTTGGTTGAATACTCATCCAAGGTTCAATGGCGAGAATTTAGAAAAGAACAAACTTTTCTATAACAAACTTGTCAACCTTGCTGCCAAGCATTCGTGCACTGCTCCTCAATTAGCTCTGGCATGGCTTCTCCACCAGGGCAATGACATAATCCCAATCCCTGGGACTACTAAAGTCAAGAACCTTGATATCAATGTTGGATCAGTAAATGTGAAGCTTGCCGAAGAGGATTTGAGAGAAATTTCTGATGCTGTGCCCATTGACGAAGTCGGGGGCAACAGAGAATTTGAACTTTTCTCAAAATTTATGTGGAACAATGCAAATACTCCATCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

345

Amino Acids

38.42

Weight (kDa)

5.55

Isoelectric Point (pI)

24.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 24 - 316 1.6e-69 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000149)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g01350 FvH4_4g01350 FvH4_4g01350 FvH4_4g01350 FvH4_4g01350 FvH4_4g01350 FvH4_4g01350 FvH4_4g01550 FvH4_4g01550 FvH4_4g01550 FvH4_6g03001 FvH4_6g03010 FvH4_6g03030 FvH4_6g03050 FvH4_6g03050 FvH4_6g03050 FvH4_6g03051 FvH4_6g03051 FvH4_6g03051 FvH4_6g03051 FvH4_6g03052 FvH4_6g03070 FvH4_6g03070 FvH4_6g03110
malus_domestica MD04G1223200.v1.1 MD08G1240600.v1.1 MD12G1239000.v1.1 MD12G1239100.v1.1 MD12G1239200.v1.1 MD12G1239300.v1.1 MD12G1239400.v1.1 MD12G1239600.v1.1 MD12G1239800.v1.1 MD12G1240000.v1.1 MD12G1240300.v1.1 MD12G1240400.v1.1 MD13G1200700.v1.1
prunus_persica Prupe.1G021900_v2.0.a1 Prupe.1G022000_v2.0.a1 Prupe.6G141300_v2.0.a1 Prupe.6G141300_v2.0.a1 Prupe.6G141300_v2.0.a1 Prupe.6G341900_v2.0.a1 Prupe.6G342100_v2.0.a1 Prupe.6G342200_v2.0.a1 Prupe.6G342300_v2.0.a1
pyrus_communis pycom04g19710 pycom12g21970 pycom12g21980 pycom12g21990 pycom12g22000 pycom12g22020 pycom13g17420 pycom16g16880
rosa_chinensis RchiOBHm_Chr3g0450451 RchiOBHm_Chr3g0451211 RchiOBHm_Chr3g0451221 RchiOBHm_Chr3g0451251 RchiOBHm_Chr3g0451261 RchiOBHm_Chr3g0451281 RchiOBHm_Chr3g0451321 RchiOBHm_Chr3g0451331 RchiOBHm_Chr3g0451341 RchiOBHm_Chr3g0454931 RchiOBHm_Chr4g0387811 RchiOBHm_Chr4g0387821 RchiOBHm_Chr4g0388101
rosa_laevigata RLG00000010080 RLG00000010092 RLG00000010093 RLG00000025402 RLG00000025666 RLG00000025667 RLG00000025668 RLG00000025669 RLG00000025672 RLG00000025673 RLG00000025674 RLG00000025675 RLG00000025676 RLG00000025678 RLG00000025679 RLG00000025729
rosa_multiflora Rmu_co8283913.1_g000001 Rmu_co8296899.1_g000001 Rmu_sc0000330.1_g000014 Rmu_sc0001792.1_g000004 Rmu_sc0002169.1_g000002 Rmu_sc0002180.1_g000028 Rmu_sc0002509.1_g000009 Rmu_sc0002575.1_g000011 Rmu_sc0002575.1_g000013 Rmu_sc0002575.1_g000015 Rmu_sc0002575.1_g000019 Rmu_sc0002575.1_g000021 Rmu_sc0002575.1_g000022 Rmu_sc0012229.1_g000010 Rmu_sc0012229.1_g000014 Rmu_sc0012229.1_g000017 Rmu_sc0012229.1_g000018 Rmu_sc0013958.1_g000001 Rmu_sc0030279.1_g000001 Rmu_sc0031850.1_g000002
rosa_roxburghii Rroxscaffold_163G00450840 Rroxscaffold_163G00450860 Rroxscaffold_163G00450870 Rroxscaffold_164G00436570 Rroxscaffold_5G00334430 Rroxscaffold_5G00334440 Rroxscaffold_5G00334650 Rroxscaffold_6G00428150 Rroxscaffold_6G00428660 Rroxscaffold_6G00428680 Rroxscaffold_6G00428710 Rroxscaffold_6G00428720 Rroxscaffold_6G00428730 Rroxscaffold_6G00428740 Rroxscaffold_6G00428750 Rroxscaffold_6G00428760
rosa_rugosa Rorug02G0535300 Rorug02G0627900 Rorug02G0632500 Rorug02G0632600 Rorug02G0633000 Rorug02G0633100 Rorug03G0003000 Rorug03G0313700 Rorug03G0314800 Rorug05G0229300 Rorug07G0155500
rosa_samantha Rh3AG028500 Rh3AG035300 Rh3AG035400 Rh3AG035500 Rh3AG035600 Rh3AG035700 Rh3AG035800 Rh3AG035900 Rh3AG062900 Rh3BG029000 Rh3BG035500 Rh3BG035600 Rh3BG035800 Rh3BG035900 Rh3BG036000 Rh3BG036100 Rh3BG036200 Rh3BG036300 Rh3BG036400 Rh3BG036500 Rh3BG036600 Rh3BG064800 Rh3CG027800 Rh3CG034200 Rh3CG034400 Rh3CG034500 Rh3CG034600 Rh3CG034700 Rh3CG034800 Rh3CG034900 Rh3CG063700 Rh3DG035100 Rh3DG035200 Rh3DG035400 Rh3DG035500 Rh3DG035600 Rh3DG035700 Rh3DG035800 Rh3DG035900 Rh3DG036000 Rh3DG036100 Rh3DG064800 Rh4AG018700 Rh4BG013600 Rh4BG013700 Rh4BG014900 Rh4CG021200 Rh4DG015600
rosa_wichuraiana Rw0G001850 Rw3G002200 Rw3G002580 Rw3G002590 Rw3G002610 Rw3G002620 Rw3G002630 Rw3G002640 Rw3G002650 Rw3G002660 Rw3G004920 Rw3G016120 Rw4G001220 Rw4G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 860
AciI CCGC 1 cut(s) 367
AclWI GGATC 1 cut(s) 907
AcsI RAATTY 5 cut(s) 278, 736, 939, 983, 1001
AcuI CTGAAG 1 cut(s) 171
AfaI GTAC 2 cut(s) 395, 482
AfiI CCNNNNNNNGG 1 cut(s) 860
AflII CTTAAG 1 cut(s) 449
AjnI CCWGG 2 cut(s) 834, 859
AjuI GAANNNNNNNTTGG 2 cut(s) 210, 242
AleI CACNNNNGTG 1 cut(s) 548
AluBI AGCT 8 cut(s) 110, 131, 356, 448, 616, 687, 815, 916
AluI AGCT 8 cut(s) 110, 131, 356, 448, 616, 687, 815, 916
Alw21I GWGCWC 1 cut(s) 800
Alw44I GTGCAC 1 cut(s) 796
AlwI GGATC 1 cut(s) 907
Ama87I CYCGRG 2 cut(s) 252, 311
ApaLI GTGCAC 1 cut(s) 796
ApeKI GCWGC 1 cut(s) 782
ApoI RAATTY 5 cut(s) 278, 736, 939, 983, 1001
Asp700I GAANNNNTTC 1 cut(s) 261
AsuC2I CCSGG 1 cut(s) 329
AsuHPI GGTGA 1 cut(s) 163
AvaI CYCGRG 2 cut(s) 252, 311
BaeGI GKGCMC 2 cut(s) 800, 957
BanII GRGCYC 1 cut(s) 526
BauI CACGAG 1 cut(s) 576
Bbv12I GWGCWC 1 cut(s) 800
BbvI GCAGC 1 cut(s) 769
BccI CCATC 2 cut(s) 548, 1036
BciT130I CCWGG 2 cut(s) 836, 861
BciVI GTATCC 1 cut(s) 427
BcnI CCSGG 1 cut(s) 329
BfaI CTAG 4 cut(s) 66, 357, 371, 680
BfmI CTRYAG 1 cut(s) 693
BfrI CTTAAG 1 cut(s) 449
BfuI GTATCC 1 cut(s) 427
BglII AGATCT 1 cut(s) 190
BisI GCNGC 2 cut(s) 368, 783
BlsI GCNGC 2 cut(s) 369, 784
Bme1390I CCNGG 3 cut(s) 329, 836, 861
BmeT110I CYCGRG 2 cut(s) 252, 311
BmiI GGNNCC 1 cut(s) 326
BmrFI CCNGG 3 cut(s) 329, 836, 861
BmrI ACTGGG 1 cut(s) 50
BmsI GCATC 2 cut(s) 177, 937
BmuI ACTGGG 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 329
BsaBI GATNNNNATC 1 cut(s) 23
BsaJI CCNNGG 5 cut(s) 646, 719, 835, 859, 860
Bsc4I CCNNNNNNNGG 1 cut(s) 860
Bse1I ACTGG 1 cut(s) 45
Bse3DI GCAATG 1 cut(s) 847
Bse8I GATNNNNATC 1 cut(s) 23
BseBI CCWGG 2 cut(s) 836, 861
BseDI CCNNGG 5 cut(s) 646, 719, 835, 859, 860
BseGI GGATG 4 cut(s) 86, 304, 535, 715
BseJI GATNNNNATC 1 cut(s) 23
BseLI CCNNNNNNNGG 1 cut(s) 860
BseMI GCAATG 1 cut(s) 847
BseNI ACTGG 1 cut(s) 45
BseRI GAGGAG 3 cut(s) 102, 105, 795
BseSI GKGCMC 2 cut(s) 800, 957
BseXI GCAGC 1 cut(s) 769
BseYI CCCAGC 1 cut(s) 131
Bsh1285I CGRYCG 1 cut(s) 652
BsiEI CGRYCG 1 cut(s) 652
BsiHKAI GWGCWC 1 cut(s) 800
BsiHKCI CYCGRG 2 cut(s) 252, 311
BsiSI CCGG 1 cut(s) 329
BslFI GGGAC 2 cut(s) 627, 877
BslI CCNNNNNNNGG 1 cut(s) 860
BsmFI GGGAC 2 cut(s) 627, 877
BsmI GAATGC 1 cut(s) 790
BsoBI CYCGRG 2 cut(s) 252, 311
Bsp1286I GDGCHC 3 cut(s) 526, 800, 957
Bsp1407I TGTACA 1 cut(s) 480
Bsp143I GATC 3 cut(s) 190, 388, 899
BspACI CCGC 1 cut(s) 367
BspHI TCATGA 2 cut(s) 124, 208
BspLI GGNNCC 1 cut(s) 326
BspMAI CTGCAG 1 cut(s) 697
BspPI GGATC 1 cut(s) 907
BspTI CTTAAG 1 cut(s) 449
BsrDI GCAATG 1 cut(s) 847
BsrGI TGTACA 1 cut(s) 480
BsrI ACTGG 1 cut(s) 45
BssECI CCNNGG 5 cut(s) 646, 719, 835, 859, 860
BssMI GATC 3 cut(s) 190, 388, 899
BssSI CACGAG 1 cut(s) 576
BssT1I CCWWGG 1 cut(s) 719
Bst2BI CACGAG 1 cut(s) 576
Bst2UI CCWGG 2 cut(s) 836, 861
Bst4CI ACNGT 2 cut(s) 246, 407
Bst6I CTCTTC 1 cut(s) 918
BstAFI CTTAAG 1 cut(s) 449
BstAUI TGTACA 1 cut(s) 480
BstC8I GCNNGC 4 cut(s) 346, 689, 693, 918
BstF5I GGATG 4 cut(s) 86, 304, 535, 715
BstKTI GATC 3 cut(s) 193, 391, 902
BstMBI GATC 3 cut(s) 190, 388, 899
BstMCI CGRYCG 1 cut(s) 652
BstNI CCWGG 2 cut(s) 836, 861
BstNSI RCATGY 1 cut(s) 348
BstSCI CCNGG 3 cut(s) 327, 834, 859
BstSFI CTRYAG 1 cut(s) 693
BstSLI GKGCMC 2 cut(s) 800, 957
BstV1I GCAGC 1 cut(s) 769
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
BsuI GTATCC 1 cut(s) 427
BtsCI GGATG 4 cut(s) 86, 304, 535, 715
BtsI GCAGTG 2 cut(s) 543, 798
BtsIMutI CAGTG 3 cut(s) 426, 543, 798
Cac8I GCNNGC 4 cut(s) 346, 689, 693, 918
CciI TCATGA 2 cut(s) 124, 208
Csp6I GTAC 2 cut(s) 394, 481
CviAII CATG 6 cut(s) 125, 209, 223, 345, 527, 822
CviQI GTAC 2 cut(s) 394, 481
DpnI GATC 3 cut(s) 192, 390, 901
DpnII GATC 3 cut(s) 190, 388, 899
Eam1104I CTCTTC 1 cut(s) 918
EarI CTCTTC 1 cut(s) 918
Eco130I CCWWGG 1 cut(s) 719
Eco24I GRGCYC 1 cut(s) 526
Eco32I GATATC 1 cut(s) 889
Eco57I CTGAAG 1 cut(s) 171
Eco88I CYCGRG 2 cut(s) 252, 311
EcoRII CCWGG 2 cut(s) 834, 859
EcoRV GATATC 1 cut(s) 889
EcoT14I CCWWGG 1 cut(s) 719
EcoT38I GRGCYC 1 cut(s) 526
ErhI CCWWGG 1 cut(s) 719
FaeI CATG 6 cut(s) 128, 212, 226, 348, 530, 825
FaqI GGGAC 2 cut(s) 627, 877
FatI CATG 6 cut(s) 124, 208, 222, 344, 526, 821
Fnu4HI GCNGC 2 cut(s) 368, 783
FokI GGATG 4 cut(s) 93, 311, 522, 702
FriOI GRGCYC 1 cut(s) 526
Fsp4HI GCNGC 2 cut(s) 368, 783
FspBI CTAG 4 cut(s) 66, 357, 371, 680
GluI GCNGC 2 cut(s) 368, 783
GsaI CCCAGC 1 cut(s) 135
HapII CCGG 1 cut(s) 329
Hin1II CATG 6 cut(s) 128, 212, 226, 348, 530, 825
HincII GTYRAC 2 cut(s) 379, 775
HindII GTYRAC 2 cut(s) 379, 775
HindIII AAGCTT 2 cut(s) 614, 914
HinfI GANTC 2 cut(s) 19, 574
HpaII CCGG 1 cut(s) 329
HphI GGTGA 1 cut(s) 163
Hpy166II GTNNAC 4 cut(s) 89, 379, 775, 798
Hpy188I TCNGA 4 cut(s) 190, 298, 496, 946
Hpy188III TCNNGA 9 cut(s) 66, 96, 125, 209, 254, 313, 578, 680, 877
Hpy8I GTNNAC 4 cut(s) 89, 379, 775, 798
HpyAV CCTTC 4 cut(s) 184, 226, 461, 664
HpyCH4III ACNGT 2 cut(s) 246, 407
HpyCH4V TGCA 4 cut(s) 553, 695, 798, 1019
Hsp92II CATG 6 cut(s) 128, 212, 226, 348, 530, 825
Kzo9I GATC 3 cut(s) 190, 388, 899
LmnI GCTCC 3 cut(s) 115, 445, 808
Lsp1109I GCAGC 1 cut(s) 769
LweI GCATC 2 cut(s) 177, 937
MaeI CTAG 4 cut(s) 66, 357, 371, 680
MalI GATC 3 cut(s) 192, 390, 901
MboI GATC 3 cut(s) 190, 388, 899
MboII GAAGA 3 cut(s) 599, 652, 935
MfeI CAATTG 2 cut(s) 266, 426
MflI RGATCY 1 cut(s) 190
MhlI GDGCHC 3 cut(s) 526, 800, 957
MluCI AATT 9 cut(s) 266, 278, 426, 621, 736, 809, 939, 983, 1001
MlyI GAGTC 1 cut(s) 568
MmeI TCCRAC 2 cut(s) 276, 877
MnlI CCTC 9 cut(s) 52, 123, 126, 261, 424, 513, 656, 816, 919
MroXI GAANNNNTTC 1 cut(s) 261
MseI TTAA 1 cut(s) 450
MslI CAYNNNNRTG 4 cut(s) 207, 213, 515, 548
MspCI CTTAAG 1 cut(s) 449
MspI CCGG 1 cut(s) 329
MspR9I CCNGG 3 cut(s) 329, 836, 861
MunI CAATTG 2 cut(s) 266, 426
Mva1269I GAATGC 1 cut(s) 790
MvaI CCWGG 2 cut(s) 836, 861
NciI CCSGG 1 cut(s) 329
NdeII GATC 3 cut(s) 190, 388, 899
NlaIII CATG 6 cut(s) 128, 212, 226, 348, 530, 825
NlaIV GGNNCC 1 cut(s) 326
NspI RCATGY 1 cut(s) 348
OliI CACNNNNGTG 1 cut(s) 548
PaeI GCATGC 1 cut(s) 348
PaeR7I CTCGAG 1 cut(s) 252
PagI TCATGA 2 cut(s) 124, 208
PasI CCCWGGG 1 cut(s) 860
PctI GAATGC 1 cut(s) 790
PdmI GAANNNNTTC 1 cut(s) 261
PfeI GAWTC 1 cut(s) 19
PflFI GACNNNGTC 1 cut(s) 965
PflMI CCANNNNNTGG 1 cut(s) 860
PkrI GCNGC 2 cut(s) 369, 784
PleI GAGTC 1 cut(s) 568
PpsI GAGTC 1 cut(s) 568
Psp6I CCWGG 2 cut(s) 834, 859
PspFI CCCAGC 1 cut(s) 131
PspGI CCWGG 2 cut(s) 834, 859
PspN4I GGNNCC 1 cut(s) 326
PstI CTGCAG 1 cut(s) 697
PsuI RGATCY 1 cut(s) 190
PsyI GACNNNGTC 1 cut(s) 965
RsaI GTAC 2 cut(s) 395, 482
RsaNI GTAC 2 cut(s) 394, 481
RseI CAYNNNNRTG 4 cut(s) 207, 213, 515, 548
SaqAI TTAA 1 cut(s) 450
SatI GCNGC 2 cut(s) 368, 783
Sau3AI GATC 3 cut(s) 190, 388, 899
SchI GAGTC 1 cut(s) 568
ScrFI CCNGG 3 cut(s) 329, 836, 861
SduI GDGCHC 3 cut(s) 526, 800, 957
SfaNI GCATC 2 cut(s) 177, 937
SfcI CTRYAG 1 cut(s) 693
Sfr274I CTCGAG 1 cut(s) 252
SlaI CTCGAG 1 cut(s) 252
SmiMI CAYNNNNRTG 4 cut(s) 207, 213, 515, 548
SmlI CTYRAG 2 cut(s) 252, 449
SmoI CTYRAG 2 cut(s) 252, 449
SphI GCATGC 1 cut(s) 348
Sse9I AATT 9 cut(s) 266, 278, 426, 621, 736, 809, 939, 983, 1001
SsiI CCGC 1 cut(s) 367
SspI AATATT 1 cut(s) 563
SspMI CTAG 4 cut(s) 66, 357, 371, 680
StyD4I CCNGG 3 cut(s) 327, 834, 859
StyI CCWWGG 1 cut(s) 719
TaaI ACNGT 2 cut(s) 246, 407
TaqI TCGA 2 cut(s) 253, 411
TaqII GACCGA 1 cut(s) 638
TasI AATT 9 cut(s) 266, 278, 426, 621, 736, 809, 939, 983, 1001
TatI WGTACW 2 cut(s) 393, 480
TauI GCSGC 1 cut(s) 370
TfiI GAWTC 1 cut(s) 19
Tru1I TTAA 1 cut(s) 450
Tru9I TTAA 1 cut(s) 450
TscAI CASTG 3 cut(s) 426, 550, 805
TseI GCWGC 1 cut(s) 782
TspDTI ATGAA 4 cut(s) 141, 231, 480, 524
TspRI CASTG 3 cut(s) 426, 550, 805
Tth111I GACNNNGTC 1 cut(s) 965
Van91I CCANNNNNTGG 1 cut(s) 860
Vha464I CTTAAG 1 cut(s) 449
VneI GTGCAC 1 cut(s) 796
XapI RAATTY 5 cut(s) 278, 736, 939, 983, 1001
XbaI TCTAGA 2 cut(s) 65, 679
XceI RCATGY 1 cut(s) 348
XhoI CTCGAG 1 cut(s) 252
XmnI GAANNNNTTC 1 cut(s) 261
XspI CTAG 4 cut(s) 66, 357, 371, 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.