FvH4_4g33571

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000056
Physical Location & Seq
Reverse (-)
31778659 .. 31779488
830 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33571.t1

Sequence Viewer

Length: 624 bp
ATGGAGGTGGAGGAGATGTTTTACTATGATCTCCATGTGGTGCAAAGATGGACATATGGGCTGATAGGCTCATGGGAGGAGGATAAACTGTTCATCAGAATGAAGTTATACAAGTGCAACTCATGGCACCTTGATACAAGAGGTGTTTTGGAGGGTCCTATGATTGCCCTAATAGATTTCTCATATCAAGCAGCAATTCATCCTGCTATTCTTGAAAAGATATTCATCATGGTTGAGGCTGCCGTCTCCCCTGTGGACGCCGTTGTGATTGTATCGGACGTCACATCCTTCCACCGAGATCTGTATTATGATCCAGTTTTGACTCCTGCGAGCAGATCATCAATCCAGGCTTTGGAGCCAGTGACAGTTTTCGATGGTAATCATAGCACCTGTGCTGTCTGTTTGGATGATTTTGAAGGAAAACCTATTACTCGGTTGCCCTGCACACACCATTTTCATGTCGACTGCATTGTTAGTGGCTGGAGATTAACCACCTTTGTCCCTACTGCCGATATCCAATGCCGACAGATGAAGACTGTGTATGGAGCTCTGTATGTGGGATTCTCCACTCTGTTTGGAGCTTTGTTTTTGTACAATCTATCATCTTTTGTTGGGGCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

208

Amino Acids

23.5

Weight (kDa)

5.23

Isoelectric Point (pI)

30.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 130 - 157 1.8e-07 Ring finger domain
zf-RING_11 PF17123 131 - 157 1.1e-08 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000056)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74620
fragaria_vesca FvH4_1g17310 FvH4_1g21860 FvH4_2g00301 FvH4_2g00500 FvH4_2g00510 FvH4_2g01040 FvH4_2g01040 FvH4_2g01040 FvH4_2g01511 FvH4_2g01521 FvH4_2g05410 FvH4_2g15860 FvH4_2g16170 FvH4_2g20442 FvH4_3g22600 FvH4_4g03111 FvH4_4g31810 FvH4_4g31840 FvH4_4g33571 FvH4_4g33580 FvH4_4g33600 FvH4_4g33620 FvH4_4g33620 FvH4_5g04820 FvH4_5g04820 FvH4_5g04821 FvH4_5g04821 FvH4_5g04830 FvH4_5g04833 FvH4_5g04833 FvH4_5g04834 FvH4_5g05562 FvH4_5g06150 FvH4_5g07970 FvH4_5g09910 FvH4_5g11280 FvH4_5g12010 FvH4_6g06910 FvH4_6g11580 FvH4_6g15110 FvH4_6g24322 FvH4_6g31360 FvH4_6g34080 FvH4_6g44500 FvH4_6g47060 FvH4_6g50581 FvH4_6g50641 FvH4_6g50650 FvH4_6g50650 FvH4_6g50981 FvH4_6g51090 FvH4_6g51180 FvH4_6g51730 FvH4_6g51740 FvH4_6g51870 FvH4_6g52331 FvH4_6g52340 FvH4_6g52350 FvH4_6g52530 FvH4_6g52530
malus_domestica MD14G1195700.v1.1
prunus_persica Prupe.6G084800_v2.0.a1 Prupe.7G084100_v2.0.a1 Prupe.8G048800_v2.0.a1
pyrus_communis pycom17g01390
rosa_chinensis RchiOBHm_Chr0c11g0499451 RchiOBHm_Chr1g0314791 RchiOBHm_Chr1g0340221 RchiOBHm_Chr1g0362121 RchiOBHm_Chr1g0362131 RchiOBHm_Chr2g0107491 RchiOBHm_Chr2g0127721 RchiOBHm_Chr2g0143901 RchiOBHm_Chr2g0161531 RchiOBHm_Chr2g0169511 RchiOBHm_Chr2g0171151 RchiOBHm_Chr2g0171221 RchiOBHm_Chr2g0171251 RchiOBHm_Chr2g0171261 RchiOBHm_Chr2g0171341 RchiOBHm_Chr2g0171561 RchiOBHm_Chr2g0171591 RchiOBHm_Chr2g0171601 RchiOBHm_Chr2g0172411 RchiOBHm_Chr2g0172441 RchiOBHm_Chr2g0172771 RchiOBHm_Chr2g0172881 RchiOBHm_Chr2g0173201 RchiOBHm_Chr2g0173211 RchiOBHm_Chr2g0173941 RchiOBHm_Chr2g0173951 RchiOBHm_Chr2g0175281 RchiOBHm_Chr3g0463081 RchiOBHm_Chr3g0482941 RchiOBHm_Chr3g0486241 RchiOBHm_Chr3g0488151 RchiOBHm_Chr3g0488191 RchiOBHm_Chr3g0488271 RchiOBHm_Chr3g0488351 RchiOBHm_Chr3g0488411 RchiOBHm_Chr3g0488491 RchiOBHm_Chr4g0410881 RchiOBHm_Chr4g0440431 RchiOBHm_Chr4g0440501 RchiOBHm_Chr6g0269041 RchiOBHm_Chr7g0194201 RchiOBHm_Chr7g0194301 RchiOBHm_Chr7g0194311 RchiOBHm_Chr7g0194391 RchiOBHm_Chr7g0194401 RchiOBHm_Chr7g0236181
rosa_laevigata RLG00000004203 RLG00000006176 RLG00000022170 RLG00000022198 RLG00000022237 RLG00000022911
rosa_multiflora Rmu_co8086672.1_g000001 Rmu_co8091660.1_g000001 Rmu_co8325445.1_g000001 Rmu_co8482323.1_g000001 Rmu_sc0000239.1_g000034 Rmu_sc0000467.1_g000001 Rmu_sc0000766.1_g000038 Rmu_sc0000789.1_g000004 Rmu_sc0000831.1_g000055 Rmu_sc0000831.1_g000056 Rmu_sc0000831.1_g000057 Rmu_sc0000831.1_g000058 Rmu_sc0000831.1_g000059 Rmu_sc0000831.1_g000060 Rmu_sc0000901.1_g000011 Rmu_sc0000901.1_g000029 Rmu_sc0001513.1_g000006 Rmu_sc0001513.1_g000011 Rmu_sc0001576.1_g000034 Rmu_sc0001685.1_g000062 Rmu_sc0002784.1_g000036 Rmu_sc0003188.1_g000026 Rmu_sc0003188.1_g000027 Rmu_sc0003314.1_g000017 Rmu_sc0003548.1_g000019 Rmu_sc0003624.1_g000018 Rmu_sc0005193.1_g000015 Rmu_sc0006328.1_g000001 Rmu_sc0006328.1_g000005 Rmu_sc0006806.1_g000014 Rmu_sc0006806.1_g000028 Rmu_sc0007320.1_g000005 Rmu_sc0007493.1_g000003 Rmu_sc0007646.1_g000011 Rmu_sc0007648.1_g000001 Rmu_sc0007661.1_g000007 Rmu_sc0007684.1_g000008 Rmu_sc0007799.1_g000007 Rmu_sc0007841.1_g000004 Rmu_sc0008051.1_g000003 Rmu_sc0008124.1_g000008 Rmu_sc0009104.1_g000008 Rmu_sc0009433.1_g000012 Rmu_sc0009883.1_g000011 Rmu_sc0010744.1_g000003 Rmu_sc0011052.1_g000003 Rmu_sc0012403.1_g000008 Rmu_sc0013831.1_g000001 Rmu_sc0016754.1_g000004 Rmu_sc0022238.1_g000002 Rmu_sc0023157.1_g000001 Rmu_sc0023894.1_g000001 Rmu_sc0028130.1_g000001 Rmu_sc0040543.1_g000001 Rmu_ssc0000252.1_g000003 Rmu_ssc0000252.1_g000004 Rmu_ssc0000261.1_g000030 Rmu_ssc0000326.1_g000007
rosa_roxburghii Rroxscaffold_157G00438560 Rroxscaffold_2G00078410 Rroxscaffold_2G00078970 Rroxscaffold_2G00079030 Rroxscaffold_2G00079120 Rroxscaffold_2G00079340 Rroxscaffold_2G00079720 Rroxscaffold_2G00079870 Rroxscaffold_2G00079910 Rroxscaffold_2G00080570 Rroxscaffold_2G00080720 Rroxscaffold_2G00080770 Rroxscaffold_2G00080780 Rroxscaffold_2G00080850 Rroxscaffold_2G00080910 Rroxscaffold_2G00116750 Rroxscaffold_3G00261370 Rroxscaffold_3G00261500 Rroxscaffold_3G00266200 Rroxscaffold_4G00305290 Rroxscaffold_4G00311110 Rroxscaffold_4G00327720 Rroxscaffold_5G00381290 Rroxscaffold_5G00381320 Rroxscaffold_6G00394220 Rroxscaffold_6G00416970
rosa_rugosa Rorug01G0164800.1 Rorug01G0164900 Rorug01G0293800 Rorug01G0293900 Rorug01G0294200 Rorug02G0144400 Rorug02G0271300 Rorug02G0271400 Rorug02G0271400 Rorug02G0271500 Rorug02G0384900 Rorug02G0384900 Rorug02G0385000 Rorug02G0385100 Rorug02G0553000 Rorug02G0553100 Rorug02G0555000 Rorug02G0560000 Rorug02G0560100 Rorug02G0561500 Rorug02G0566500 Rorug02G0568800 Rorug02G0569000 Rorug02G0569400 Rorug02G0569700 Rorug02G0575200 Rorug02G0582300 Rorug03G0061300 Rorug03G0126300 Rorug03G0202400 Rorug03G0224200 Rorug03G0238200 Rorug03G0238300 Rorug03G0238400 Rorug03G0255100 Rorug03G0255100 Rorug04G0326000 Rorug04G0326200 Rorug04G0326300 Rorug06G0124000 Rorug07G0015300 Rorug07G0015700 Rorug07G0015700 Rorug07G0265000
rosa_samantha Rh1AG304100 Rh1AG304500 Rh1AG304600 Rh1CG166600 Rh1CG285500 Rh1DG218600 Rh2AG323600 Rh2AG611800 Rh2AG626100 Rh2AG626400 Rh2AG627700 Rh2AG629700 Rh2AG634400 Rh2AG634500 Rh2AG634800 Rh2AG652800 Rh2BG206800 Rh2BG636300 Rh2BG636400 Rh2BG637300 Rh2BG637400 Rh2BG642600 Rh2BG648000 Rh2BG648300 Rh2BG652700 Rh2BG664000 Rh2CG311300 Rh2CG604800 Rh2CG605400 Rh2CG606300 Rh2CG606800 Rh2CG623800 Rh2CG628700 Rh2DG201600 Rh2DG351100 Rh2DG648000 Rh2DG648100 Rh2DG648900 Rh2DG649000 Rh2DG649100 Rh2DG651000 Rh2DG654900 Rh2DG655100 Rh2DG655200 Rh2DG656700 Rh2DG656900 Rh2DG657100 Rh2DG662700 Rh2DG662900 Rh2DG666300 Rh2DG667000 Rh2DG670700 Rh2DG672100 Rh2DG673400 Rh2DG673600 Rh2DG675200 Rh2DG675300 Rh2DG675600 Rh2DG679000 Rh2DG679100 Rh3AG048000 Rh3AG048100 Rh3AG106800 Rh3AG119500 Rh3AG251300 Rh3AG285800 Rh3AG286700 Rh3AG287300 Rh3AG287400 Rh3CG049400 Rh3CG321600 Rh3DG320300 Rh4AG377300 Rh4AG377700 Rh4AG377900 Rh4DG159200 Rh7AG064400 Rh7AG097600 Rh7AG141500 Rh7AG142100 Rh7AG142200 Rh7AG142300 Rh7AG142600 Rh7AG142800 Rh7AG417800 Rh7AG424000 Rh7CG147100 Rh7DG143000 Rh7DG143900 Rh7DG144400 Rh7DG144700
rosa_wichuraiana Rw0G019520 Rw1G014950 Rw1G027080 Rw2G015390 Rw2G035650 Rw2G046060 Rw2G050780 Rw2G050810 Rw2G051810 Rw2G051850 Rw2G051940 Rw2G051990 Rw2G052000 Rw2G052130 Rw2G052140 Rw2G052530 Rw2G052760 Rw2G052830 Rw2G053180 Rw2G053330 Rw2G053630 Rw3G010110 Rw3G024300 Rw3G025400 Rw3G025450 Rw4G032320 Rw4G032440 Rw6G037400 Rw7G012180 Rw7G012220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 282
AccB1I GGYRCC 1 cut(s) 126
AccB7I CCANNNNNTGG 1 cut(s) 352
AccI GTMKAC 1 cut(s) 462
AclWI GGATC 1 cut(s) 305
AcyI GRCGYC 2 cut(s) 258, 279
AfaI GTAC 1 cut(s) 593
AfiI CCNNNNNNNGG 1 cut(s) 352
AgsI TTSAA 2 cut(s) 215, 416
AjnI CCWGG 1 cut(s) 345
AloI GAACNNNNNNTCC 2 cut(s) 74, 106
AluBI AGCT 2 cut(s) 548, 581
AluI AGCT 2 cut(s) 548, 581
Alw21I GWGCWC 1 cut(s) 550
Alw26I GTCTC 1 cut(s) 250
AlwI GGATC 1 cut(s) 305
ApeKI GCWGC 2 cut(s) 191, 239
AspS9I GGNCC 1 cut(s) 155
AvaII GGWCC 1 cut(s) 155
BanI GGYRCC 1 cut(s) 126
BanII GRGCYC 1 cut(s) 550
BbsI GAAGAC 1 cut(s) 539
Bbv12I GWGCWC 1 cut(s) 550
BbvI GCAGC 2 cut(s) 203, 226
BccI CCATC 2 cut(s) 42, 368
BceAI ACGGC 2 cut(s) 227, 245
BciT130I CCWGG 1 cut(s) 347
BcoDI GTCTC 1 cut(s) 250
BglII AGATCT 1 cut(s) 298
BisI GCNGC 2 cut(s) 192, 240
BlsI GCNGC 2 cut(s) 193, 241
Bme1390I CCNGG 1 cut(s) 347
Bme18I GGWCC 1 cut(s) 155
BmgT120I GGNCC 1 cut(s) 155
BmiI GGNNCC 3 cut(s) 128, 156, 357
BmrFI CCNGG 1 cut(s) 347
BpiI GAAGAC 1 cut(s) 539
BpmI CTGGAG 1 cut(s) 502
BsaBI GATNNNNATC 2 cut(s) 224, 378
BsaHI GRCGYC 2 cut(s) 258, 279
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 1 cut(s) 352
Bse1I ACTGG 2 cut(s) 314, 359
Bse8I GATNNNNATC 2 cut(s) 224, 378
BseBI CCWGG 1 cut(s) 347
BseGI GGATG 3 cut(s) 199, 284, 412
BseJI GATNNNNATC 2 cut(s) 224, 378
BseLI CCNNNNNNNGG 1 cut(s) 352
BseNI ACTGG 2 cut(s) 314, 359
BseRI GAGGAG 2 cut(s) 26, 92
BseXI GCAGC 2 cut(s) 203, 226
BsgI GTGCAG 1 cut(s) 427
BshNI GGYRCC 1 cut(s) 126
BsiHKAI GWGCWC 1 cut(s) 550
BslFI GGGAC 1 cut(s) 485
BslI CCNNNNNNNGG 1 cut(s) 352
BsmAI GTCTC 1 cut(s) 250
BsmBI CGTCTC 1 cut(s) 250
BsmFI GGGAC 1 cut(s) 485
Bsp1286I GDGCHC 1 cut(s) 550
Bsp1407I TGTACA 1 cut(s) 591
Bsp143I GATC 4 cut(s) 28, 298, 310, 335
BspLI GGNNCC 3 cut(s) 128, 156, 357
BspPI GGATC 1 cut(s) 305
BspT107I GGYRCC 1 cut(s) 126
BsrGI TGTACA 1 cut(s) 591
BsrI ACTGG 2 cut(s) 314, 359
BssMI GATC 4 cut(s) 28, 298, 310, 335
BssNI GRCGYC 2 cut(s) 258, 279
Bst2UI CCWGG 1 cut(s) 347
Bst4CI ACNGT 3 cut(s) 90, 367, 538
BstACI GRCGYC 2 cut(s) 258, 279
BstAUI TGTACA 1 cut(s) 591
BstC8I GCNNGC 1 cut(s) 331
BstF5I GGATG 3 cut(s) 199, 284, 412
BstKTI GATC 4 cut(s) 31, 301, 313, 338
BstMAI GTCTC 1 cut(s) 250
BstMBI GATC 4 cut(s) 28, 298, 310, 335
BstNI CCWGG 1 cut(s) 347
BstSCI CCNGG 1 cut(s) 345
BstV1I GCAGC 2 cut(s) 203, 226
BstV2I GAAGAC 1 cut(s) 539
BstX2I RGATCY 1 cut(s) 298
BstYI RGATCY 1 cut(s) 298
BtsCI GGATG 3 cut(s) 199, 284, 412
BtsIMutI CAGTG 1 cut(s) 366
Cac8I GCNNGC 1 cut(s) 331
Cfr13I GGNCC 1 cut(s) 155
CseI GACGC 1 cut(s) 266
Csp6I GTAC 1 cut(s) 592
CspCI CAANNNNNGTGG 2 cut(s) 556, 591
CviAII CATG 5 cut(s) 35, 72, 123, 229, 458
CviJI RGCY 8 cut(s) 61, 69, 239, 350, 358, 480, 548, 581
CviKI_1 RGCY 8 cut(s) 61, 69, 239, 350, 358, 480, 548, 581
CviQI GTAC 1 cut(s) 592
DpnI GATC 4 cut(s) 30, 300, 312, 337
DpnII GATC 4 cut(s) 28, 298, 310, 335
Ecl136II GAGCTC 1 cut(s) 548
Eco24I GRGCYC 1 cut(s) 550
Eco32I GATATC 1 cut(s) 514
Eco47I GGWCC 1 cut(s) 155
Eco53kI GAGCTC 1 cut(s) 548
EcoICRI GAGCTC 1 cut(s) 548
EcoO109I RGGNCCY 1 cut(s) 155
EcoRII CCWGG 1 cut(s) 345
EcoRV GATATC 1 cut(s) 514
EcoT38I GRGCYC 1 cut(s) 550
Esp3I CGTCTC 1 cut(s) 250
FaeI CATG 5 cut(s) 38, 75, 126, 232, 461
FaqI GGGAC 1 cut(s) 485
FatI CATG 5 cut(s) 34, 71, 122, 228, 457
FauNDI CATATG 1 cut(s) 55
FblI GTMKAC 1 cut(s) 462
Fnu4HI GCNGC 2 cut(s) 192, 240
FokI GGATG 3 cut(s) 186, 271, 419
FriOI GRGCYC 1 cut(s) 550
Fsp4HI GCNGC 2 cut(s) 192, 240
GluI GCNGC 2 cut(s) 192, 240
GsuI CTGGAG 1 cut(s) 502
HgaI GACGC 1 cut(s) 266
Hin1I GRCGYC 2 cut(s) 258, 279
Hin1II CATG 5 cut(s) 38, 75, 126, 232, 461
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HinfI GANTC 2 cut(s) 322, 561
Hpy166II GTNNAC 2 cut(s) 256, 463
Hpy188I TCNGA 2 cut(s) 98, 277
Hpy188III TCNNGA 1 cut(s) 212
Hpy8I GTNNAC 2 cut(s) 256, 463
HpyAV CCTTC 2 cut(s) 298, 410
HpyCH4III ACNGT 3 cut(s) 90, 367, 538
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 4 cut(s) 43, 117, 444, 468
HpySE526I ACGT 1 cut(s) 279
Hsp92I GRCGYC 2 cut(s) 258, 279
Hsp92II CATG 5 cut(s) 38, 75, 126, 232, 461
Kzo9I GATC 4 cut(s) 28, 298, 310, 335
LmnI GCTCC 3 cut(s) 355, 545, 578
Lsp1109I GCAGC 2 cut(s) 203, 226
MaeII ACGT 1 cut(s) 279
MaeIII GTNAC 2 cut(s) 280, 361
MalI GATC 4 cut(s) 30, 300, 312, 337
MboI GATC 4 cut(s) 28, 298, 310, 335
MboII GAAGA 1 cut(s) 544
MflI RGATCY 1 cut(s) 298
MhlI GDGCHC 1 cut(s) 550
MluCI AATT 1 cut(s) 195
MlyI GAGTC 1 cut(s) 316
MnlI CCTC 6 cut(s) 4, 70, 73, 134, 145, 229
MseI TTAA 1 cut(s) 488
MslI CAYNNNNRTG 2 cut(s) 98, 456
MspR9I CCNGG 1 cut(s) 347
MvaI CCWGG 1 cut(s) 347
NdeI CATATG 1 cut(s) 55
NdeII GATC 4 cut(s) 28, 298, 310, 335
NlaIII CATG 5 cut(s) 38, 75, 126, 232, 461
NlaIV GGNNCC 3 cut(s) 128, 156, 357
NmuCI GTSAC 2 cut(s) 280, 361
PfeI GAWTC 1 cut(s) 561
PflMI CCANNNNNTGG 1 cut(s) 352
PkrI GCNGC 2 cut(s) 193, 241
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
PpuMI RGGWCCY 1 cut(s) 155
Psp124BI GAGCTC 1 cut(s) 550
Psp5II RGGWCCY 1 cut(s) 155
Psp6I CCWGG 1 cut(s) 345
PspGI CCWGG 1 cut(s) 345
PspN4I GGNNCC 3 cut(s) 128, 156, 357
PspPI GGNCC 1 cut(s) 155
PspPPI RGGWCCY 1 cut(s) 155
PsuI RGATCY 1 cut(s) 298
RsaI GTAC 1 cut(s) 593
RsaNI GTAC 1 cut(s) 592
RseI CAYNNNNRTG 2 cut(s) 98, 456
SacI GAGCTC 1 cut(s) 550
SalI GTCGAC 1 cut(s) 461
SaqAI TTAA 1 cut(s) 488
SatI GCNGC 2 cut(s) 192, 240
Sau3AI GATC 4 cut(s) 28, 298, 310, 335
Sau96I GGNCC 1 cut(s) 155
SchI GAGTC 1 cut(s) 316
ScrFI CCNGG 1 cut(s) 347
SduI GDGCHC 1 cut(s) 550
SetI ASST 9 cut(s) 9, 132, 145, 282, 392, 427, 497, 550, 583
SinI GGWCC 1 cut(s) 155
SmiMI CAYNNNNRTG 2 cut(s) 98, 456
Sse9I AATT 1 cut(s) 195
SstI GAGCTC 1 cut(s) 550
StyD4I CCNGG 1 cut(s) 345
TaaI ACNGT 3 cut(s) 90, 367, 538
TaiI ACGT 1 cut(s) 282
TaqI TCGA 2 cut(s) 372, 462
TasI AATT 1 cut(s) 195
TatI WGTACW 1 cut(s) 591
TfiI GAWTC 1 cut(s) 561
Tru1I TTAA 1 cut(s) 488
Tru9I TTAA 1 cut(s) 488
TscAI CASTG 1 cut(s) 366
TseFI GTSAC 2 cut(s) 280, 361
TseI GCWGC 2 cut(s) 191, 239
Tsp45I GTSAC 2 cut(s) 280, 361
TspDTI ATGAA 6 cut(s) 82, 116, 188, 214, 446, 545
TspRI CASTG 1 cut(s) 366
Van91I CCANNNNNTGG 1 cut(s) 352
VpaK11BI GGWCC 1 cut(s) 155
XmiI GTMKAC 1 cut(s) 462
ZraI GACGTC 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.