ubiquitin-protein transferase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 259 |
| AccII | CGCG | 2 cut(s) | 81, 489 |
| AciI | CCGC | 1 cut(s) | 596 |
| AclWI | GGATC | 1 cut(s) | 843 |
| AcoI | YGGCCR | 1 cut(s) | 63 |
| AcsI | RAATTY | 1 cut(s) | 794 |
| AfaI | GTAC | 2 cut(s) | 603, 865 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 259 |
| AflIII | ACRYGT | 2 cut(s) | 371, 860 |
| AgsI | TTSAA | 9 cut(s) | 50, 59, 177, 281, 662, 676, 742, 749, 915 |
| AjnI | CCWGG | 1 cut(s) | 33 |
| AluBI | AGCT | 7 cut(s) | 98, 315, 350, 418, 584, 619, 665 |
| AluI | AGCT | 7 cut(s) | 98, 315, 350, 418, 584, 619, 665 |
| Alw21I | GWGCWC | 1 cut(s) | 579 |
| Alw26I | GTCTC | 1 cut(s) | 322 |
| Alw44I | GTGCAC | 1 cut(s) | 575 |
| AlwI | GGATC | 1 cut(s) | 843 |
| AoxI | GGCC | 2 cut(s) | 63, 768 |
| ApaLI | GTGCAC | 1 cut(s) | 575 |
| ApeKI | GCWGC | 1 cut(s) | 22 |
| ApoI | RAATTY | 1 cut(s) | 794 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 779, 811 |
| AseI | ATTAAT | 1 cut(s) | 899 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 276 |
| AspLEI | GCGC | 1 cut(s) | 114 |
| AsuHPI | GGTGA | 2 cut(s) | 493, 776 |
| AsuII | TTCGAA | 1 cut(s) | 668 |
| BaeGI | GKGCMC | 2 cut(s) | 497, 579 |
| BalI | TGGCCA | 1 cut(s) | 65 |
| BauI | CACGAG | 1 cut(s) | 197 |
| Bbv12I | GWGCWC | 1 cut(s) | 579 |
| BbvI | GCAGC | 1 cut(s) | 34 |
| BccI | CCATC | 3 cut(s) | 74, 152, 395 |
| BciT130I | CCWGG | 1 cut(s) | 35 |
| BclI | TGATCA | 2 cut(s) | 133, 607 |
| BcoDI | GTCTC | 1 cut(s) | 322 |
| BfaI | CTAG | 2 cut(s) | 476, 635 |
| BisI | GCNGC | 2 cut(s) | 23, 596 |
| BlsI | GCNGC | 2 cut(s) | 24, 597 |
| Bme1390I | CCNGG | 1 cut(s) | 35 |
| BmiI | GGNNCC | 1 cut(s) | 807 |
| BmrFI | CCNGG | 1 cut(s) | 35 |
| BpmI | CTGGAG | 1 cut(s) | 56 |
| Bpu14I | TTCGAA | 1 cut(s) | 668 |
| BpuEI | CTTGAG | 2 cut(s) | 738, 768 |
| Bsa29I | ATCGAT | 1 cut(s) | 147 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 29, 59 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 259 |
| BseBI | CCWGG | 1 cut(s) | 35 |
| BseCI | ATCGAT | 1 cut(s) | 147 |
| BseGI | GGATG | 3 cut(s) | 66, 166, 394 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 259 |
| BseMII | CTCAG | 1 cut(s) | 555 |
| BseRI | GAGGAG | 1 cut(s) | 32 |
| BseSI | GKGCMC | 2 cut(s) | 497, 579 |
| BseXI | GCAGC | 1 cut(s) | 34 |
| Bsh1236I | CGCG | 2 cut(s) | 81, 489 |
| BshFI | GGCC | 2 cut(s) | 65, 770 |
| BshVI | ATCGAT | 1 cut(s) | 147 |
| BsiHKAI | GWGCWC | 1 cut(s) | 579 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 259 |
| BsmAI | GTCTC | 1 cut(s) | 322 |
| BsmI | GAATGC | 1 cut(s) | 12 |
| BsnI | GGCC | 2 cut(s) | 65, 770 |
| Bsp119I | TTCGAA | 1 cut(s) | 668 |
| Bsp1286I | GDGCHC | 2 cut(s) | 497, 579 |
| Bsp143I | GATC | 4 cut(s) | 133, 412, 607, 835 |
| BspACI | CCGC | 1 cut(s) | 596 |
| BspANI | GGCC | 2 cut(s) | 65, 770 |
| BspCNI | CTCAG | 1 cut(s) | 554 |
| BspDI | ATCGAT | 1 cut(s) | 147 |
| BspFNI | CGCG | 2 cut(s) | 81, 489 |
| BspHI | TCATGA | 1 cut(s) | 244 |
| BspLI | GGNNCC | 1 cut(s) | 807 |
| BspPI | GGATC | 1 cut(s) | 843 |
| BspT104I | TTCGAA | 1 cut(s) | 668 |
| BssMI | GATC | 4 cut(s) | 133, 412, 607, 835 |
| BssSI | CACGAG | 1 cut(s) | 197 |
| Bst2BI | CACGAG | 1 cut(s) | 197 |
| Bst2UI | CCWGG | 1 cut(s) | 35 |
| Bst4CI | ACNGT | 2 cut(s) | 45, 109 |
| BstBI | TTCGAA | 1 cut(s) | 668 |
| BstC8I | GCNNGC | 1 cut(s) | 621 |
| BstDEI | CTNAG | 1 cut(s) | 541 |
| BstF5I | GGATG | 3 cut(s) | 66, 166, 394 |
| BstFNI | CGCG | 2 cut(s) | 81, 489 |
| BstHHI | GCGC | 1 cut(s) | 114 |
| BstKTI | GATC | 4 cut(s) | 136, 415, 610, 838 |
| BstMAI | GTCTC | 1 cut(s) | 322 |
| BstMBI | GATC | 4 cut(s) | 133, 412, 607, 835 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 574 |
| BstNI | CCWGG | 1 cut(s) | 35 |
| BstNSI | RCATGY | 1 cut(s) | 375 |
| BstSCI | CCNGG | 1 cut(s) | 33 |
| BstSLI | GKGCMC | 2 cut(s) | 497, 579 |
| BstUI | CGCG | 2 cut(s) | 81, 489 |
| BstV1I | GCAGC | 1 cut(s) | 34 |
| Bsu15I | ATCGAT | 1 cut(s) | 147 |
| BsuRI | GGCC | 2 cut(s) | 65, 770 |
| BsuTUI | ATCGAT | 1 cut(s) | 147 |
| BtsCI | GGATG | 3 cut(s) | 66, 166, 394 |
| BtsI | GCAGTG | 1 cut(s) | 457 |
| BtsIMutI | CAGTG | 2 cut(s) | 457, 552 |
| Cac8I | GCNNGC | 1 cut(s) | 621 |
| CciI | TCATGA | 1 cut(s) | 244 |
| CfoI | GCGC | 1 cut(s) | 114 |
| ClaI | ATCGAT | 1 cut(s) | 147 |
| CseI | GACGC | 2 cut(s) | 65, 70 |
| Csp6I | GTAC | 2 cut(s) | 602, 864 |
| CviAII | CATG | 8 cut(s) | 73, 245, 254, 267, 372, 452, 686, 818 |
| CviQI | GTAC | 2 cut(s) | 602, 864 |
| DdeI | CTNAG | 1 cut(s) | 541 |
| DpnI | GATC | 4 cut(s) | 135, 414, 609, 837 |
| DpnII | GATC | 4 cut(s) | 133, 412, 607, 835 |
| EaeI | YGGCCR | 1 cut(s) | 63 |
| Eco32I | GATATC | 2 cut(s) | 250, 532 |
| EcoRII | CCWGG | 1 cut(s) | 33 |
| EcoRV | GATATC | 2 cut(s) | 250, 532 |
| FaeI | CATG | 8 cut(s) | 76, 248, 257, 270, 375, 455, 689, 821 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 315, 347 |
| FatI | CATG | 8 cut(s) | 72, 244, 253, 266, 371, 451, 685, 817 |
| FbaI | TGATCA | 2 cut(s) | 133, 607 |
| Fnu4HI | GCNGC | 2 cut(s) | 23, 596 |
| FokI | GGATG | 3 cut(s) | 53, 153, 401 |
| Fsp4HI | GCNGC | 2 cut(s) | 23, 596 |
| FspBI | CTAG | 2 cut(s) | 476, 635 |
| GlaI | GCGC | 1 cut(s) | 113 |
| GluI | GCNGC | 2 cut(s) | 23, 596 |
| GsuI | CTGGAG | 1 cut(s) | 56 |
| HaeIII | GGCC | 2 cut(s) | 65, 770 |
| HgaI | GACGC | 2 cut(s) | 65, 70 |
| HhaI | GCGC | 1 cut(s) | 114 |
| Hin1II | CATG | 8 cut(s) | 76, 248, 257, 270, 375, 455, 689, 821 |
| Hin6I | GCGC | 1 cut(s) | 112 |
| HinP1I | GCGC | 1 cut(s) | 112 |
| HincII | GTYRAC | 1 cut(s) | 855 |
| HindII | GTYRAC | 1 cut(s) | 855 |
| HindIII | AAGCTT | 1 cut(s) | 663 |
| HinfI | GANTC | 4 cut(s) | 272, 484, 650, 749 |
| HpaI | GTTAAC | 1 cut(s) | 855 |
| HphI | GGTGA | 2 cut(s) | 493, 776 |
| Hpy166II | GTNNAC | 5 cut(s) | 196, 577, 718, 855, 864 |
| Hpy188I | TCNGA | 1 cut(s) | 544 |
| Hpy188III | TCNNGA | 5 cut(s) | 137, 197, 245, 755, 785 |
| Hpy8I | GTNNAC | 5 cut(s) | 196, 577, 718, 855, 864 |
| Hpy99I | CGWCG | 1 cut(s) | 81 |
| HpyCH4III | ACNGT | 2 cut(s) | 45, 109 |
| HpyCH4IV | ACGT | 1 cut(s) | 860 |
| HpyCH4V | TGCA | 6 cut(s) | 308, 440, 455, 462, 512, 577 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 574 |
| HpyF3I | CTNAG | 1 cut(s) | 541 |
| HpySE526I | ACGT | 1 cut(s) | 860 |
| Hsp92II | CATG | 8 cut(s) | 76, 248, 257, 270, 375, 455, 689, 821 |
| HspAI | GCGC | 1 cut(s) | 112 |
| Ksp22I | TGATCA | 2 cut(s) | 133, 607 |
| KspAI | GTTAAC | 1 cut(s) | 855 |
| Kzo9I | GATC | 4 cut(s) | 133, 412, 607, 835 |
| LmnI | GCTCC | 1 cut(s) | 19 |
| LpnPI | CCDG | 9 cut(s) | 20, 47, 122, 318, 404, 448, 585, 618, 788 |
| Lsp1109I | GCAGC | 1 cut(s) | 34 |
| MaeI | CTAG | 2 cut(s) | 476, 635 |
| MaeII | ACGT | 1 cut(s) | 860 |
| MaeIII | GTNAC | 2 cut(s) | 103, 262 |
| MalI | GATC | 4 cut(s) | 135, 414, 609, 837 |
| MboI | GATC | 4 cut(s) | 133, 412, 607, 835 |
| MboII | GAAGA | 2 cut(s) | 356, 859 |
| MhlI | GDGCHC | 2 cut(s) | 497, 579 |
| MlsI | TGGCCA | 1 cut(s) | 65 |
| MluCI | AATT | 5 cut(s) | 182, 546, 586, 730, 794 |
| MluNI | TGGCCA | 1 cut(s) | 65 |
| MmeI | TCCRAC | 1 cut(s) | 392 |
| MnlI | CCTC | 9 cut(s) | 10, 205, 226, 472, 517, 550, 713, 754, 760 |
| Mox20I | TGGCCA | 1 cut(s) | 65 |
| MroXI | GAANNNNTTC | 1 cut(s) | 276 |
| MscI | TGGCCA | 1 cut(s) | 65 |
| MseI | TTAA | 3 cut(s) | 854, 899, 922 |
| MslI | CAYNNNNRTG | 1 cut(s) | 71 |
| Msp20I | TGGCCA | 1 cut(s) | 65 |
| MspA1I | CMGCKG | 1 cut(s) | 418 |
| MspR9I | CCNGG | 1 cut(s) | 35 |
| Mva1269I | GAATGC | 1 cut(s) | 12 |
| MvaI | CCWGG | 1 cut(s) | 35 |
| MvnI | CGCG | 2 cut(s) | 81, 489 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 574 |
| NdeII | GATC | 4 cut(s) | 133, 412, 607, 835 |
| NlaIII | CATG | 8 cut(s) | 76, 248, 257, 270, 375, 455, 689, 821 |
| NlaIV | GGNNCC | 1 cut(s) | 807 |
| NmuCI | GTSAC | 1 cut(s) | 103 |
| NspI | RCATGY | 1 cut(s) | 375 |
| NspV | TTCGAA | 1 cut(s) | 668 |
| PagI | TCATGA | 1 cut(s) | 244 |
| PciI | ACATGT | 1 cut(s) | 371 |
| PctI | GAATGC | 1 cut(s) | 12 |
| PdmI | GAANNNNTTC | 1 cut(s) | 276 |
| PfeI | GAWTC | 4 cut(s) | 272, 484, 650, 749 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 259 |
| PfoI | TCCNGGA | 1 cut(s) | 33 |
| PkrI | GCNGC | 2 cut(s) | 24, 597 |
| PscI | ACATGT | 1 cut(s) | 371 |
| PshBI | ATTAAT | 1 cut(s) | 899 |
| Psp6I | CCWGG | 1 cut(s) | 33 |
| PspGI | CCWGG | 1 cut(s) | 33 |
| PspN4I | GGNNCC | 1 cut(s) | 807 |
| PvuII | CAGCTG | 1 cut(s) | 418 |
| RsaI | GTAC | 2 cut(s) | 603, 865 |
| RsaNI | GTAC | 2 cut(s) | 602, 864 |
| RseI | CAYNNNNRTG | 1 cut(s) | 71 |
| SaqAI | TTAA | 3 cut(s) | 854, 899, 922 |
| SatI | GCNGC | 2 cut(s) | 23, 596 |
| Sau3AI | GATC | 4 cut(s) | 133, 412, 607, 835 |
| ScrFI | CCNGG | 1 cut(s) | 35 |
| SduI | GDGCHC | 2 cut(s) | 497, 579 |
| SfuI | TTCGAA | 1 cut(s) | 668 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 71 |
| SmlI | CTYRAG | 2 cut(s) | 753, 783 |
| SmoI | CTYRAG | 2 cut(s) | 753, 783 |
| Sse9I | AATT | 5 cut(s) | 182, 546, 586, 730, 794 |
| SsiI | CCGC | 1 cut(s) | 596 |
| SspI | AATATT | 1 cut(s) | 814 |
| SspMI | CTAG | 2 cut(s) | 476, 635 |
| StyD4I | CCNGG | 1 cut(s) | 33 |
| TaaI | ACNGT | 2 cut(s) | 45, 109 |
| TaiI | ACGT | 1 cut(s) | 863 |
| TaqI | TCGA | 4 cut(s) | 147, 505, 534, 668 |
| TasI | AATT | 5 cut(s) | 182, 546, 586, 730, 794 |
| TauI | GCSGC | 1 cut(s) | 598 |
| TfiI | GAWTC | 4 cut(s) | 272, 484, 650, 749 |
| Tru1I | TTAA | 3 cut(s) | 854, 899, 922 |
| Tru9I | TTAA | 3 cut(s) | 854, 899, 922 |
| TscAI | CASTG | 2 cut(s) | 464, 559 |
| TseFI | GTSAC | 1 cut(s) | 103 |
| TseI | GCWGC | 1 cut(s) | 22 |
| Tsp45I | GTSAC | 1 cut(s) | 103 |
| TspDTI | ATGAA | 4 cut(s) | 440, 642, 806, 807 |
| TspGWI | ACGGA | 2 cut(s) | 848, 881 |
| TspRI | CASTG | 2 cut(s) | 464, 559 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 259 |
| VneI | GTGCAC | 1 cut(s) | 575 |
| VspI | ATTAAT | 1 cut(s) | 899 |
| XapI | RAATTY | 1 cut(s) | 794 |
| XceI | RCATGY | 1 cut(s) | 375 |
| XmnI | GAANNNNTTC | 1 cut(s) | 276 |
| XspI | CTAG | 2 cut(s) | 476, 635 |