Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 283 |
| AciI | CCGC | 2 cut(s) | 132, 283 |
| AclWI | GGATC | 2 cut(s) | 414, 423 |
| AcoI | YGGCCR | 1 cut(s) | 348 |
| AcsI | RAATTY | 1 cut(s) | 104 |
| AfaI | GTAC | 1 cut(s) | 33 |
| AflIII | ACRYGT | 1 cut(s) | 385 |
| AgsI | TTSAA | 3 cut(s) | 109, 404, 593 |
| AjuI | GAANNNNNNNTTGG | 1 cut(s) | 693 |
| AluBI | AGCT | 4 cut(s) | 197, 232, 491, 596 |
| AluI | AGCT | 4 cut(s) | 197, 232, 491, 596 |
| Alw21I | GWGCWC | 3 cut(s) | 199, 264, 342 |
| Alw26I | GTCTC | 1 cut(s) | 223 |
| AlwI | GGATC | 2 cut(s) | 414, 423 |
| AoxI | GGCC | 3 cut(s) | 348, 476, 523 |
| ApeKI | GCWGC | 2 cut(s) | 361, 488 |
| ApoI | RAATTY | 1 cut(s) | 104 |
| AspLEI | GCGC | 1 cut(s) | 251 |
| AspS9I | GGNCC | 1 cut(s) | 523 |
| AsuHPI | GGTGA | 1 cut(s) | 596 |
| BalI | TGGCCA | 1 cut(s) | 350 |
| BanII | GRGCYC | 1 cut(s) | 199 |
| BauI | CACGAG | 1 cut(s) | 222 |
| Bbv12I | GWGCWC | 3 cut(s) | 199, 264, 342 |
| BbvI | GCAGC | 2 cut(s) | 348, 500 |
| BccI | CCATC | 2 cut(s) | 107, 280 |
| BciVI | GTATCC | 1 cut(s) | 454 |
| BclI | TGATCA | 1 cut(s) | 54 |
| BcoDI | GTCTC | 1 cut(s) | 223 |
| BfaI | CTAG | 1 cut(s) | 42 |
| BfmI | CTRYAG | 1 cut(s) | 486 |
| BfuI | GTATCC | 1 cut(s) | 454 |
| BisI | GCNGC | 2 cut(s) | 362, 489 |
| BlsI | GCNGC | 2 cut(s) | 363, 490 |
| BmgT120I | GGNCC | 1 cut(s) | 523 |
| BmsI | GCATC | 1 cut(s) | 260 |
| BpuEI | CTTGAG | 1 cut(s) | 183 |
| Bsa29I | ATCGAT | 1 cut(s) | 418 |
| BsaI | GGTCTC | 1 cut(s) | 223 |
| BsaJI | CCNNGG | 2 cut(s) | 437, 456 |
| Bse1I | ACTGG | 2 cut(s) | 643, 657 |
| BseCI | ATCGAT | 1 cut(s) | 418 |
| BseDI | CCNNGG | 2 cut(s) | 437, 456 |
| BseGI | GGATG | 3 cut(s) | 251, 291, 295 |
| BseNI | ACTGG | 2 cut(s) | 643, 657 |
| BseXI | GCAGC | 2 cut(s) | 348, 500 |
| Bsh1236I | CGCG | 1 cut(s) | 283 |
| BshFI | GGCC | 3 cut(s) | 350, 478, 525 |
| BshVI | ATCGAT | 1 cut(s) | 418 |
| BsiHKAI | GWGCWC | 3 cut(s) | 199, 264, 342 |
| BslFI | GGGAC | 4 cut(s) | 55, 143, 152, 278 |
| BsmAI | GTCTC | 1 cut(s) | 223 |
| BsmFI | GGGAC | 4 cut(s) | 55, 143, 152, 278 |
| BsnI | GGCC | 3 cut(s) | 350, 478, 525 |
| Bso31I | GGTCTC | 1 cut(s) | 223 |
| Bsp1286I | GDGCHC | 3 cut(s) | 199, 264, 342 |
| Bsp143I | GATC | 4 cut(s) | 54, 415, 419, 424 |
| Bsp19I | CCATGG | 1 cut(s) | 456 |
| BspACI | CCGC | 2 cut(s) | 132, 283 |
| BspANI | GGCC | 3 cut(s) | 350, 478, 525 |
| BspDI | ATCGAT | 1 cut(s) | 418 |
| BspFNI | CGCG | 1 cut(s) | 283 |
| BspMAI | CTGCAG | 1 cut(s) | 490 |
| BspPI | GGATC | 2 cut(s) | 414, 423 |
| BspTNI | GGTCTC | 1 cut(s) | 223 |
| BsrI | ACTGG | 2 cut(s) | 643, 657 |
| BssECI | CCNNGG | 2 cut(s) | 437, 456 |
| BssMI | GATC | 4 cut(s) | 54, 415, 419, 424 |
| BssSI | CACGAG | 1 cut(s) | 222 |
| BssT1I | CCWWGG | 1 cut(s) | 456 |
| Bst2BI | CACGAG | 1 cut(s) | 222 |
| Bst4CI | ACNGT | 2 cut(s) | 259, 313 |
| BstC8I | GCNNGC | 1 cut(s) | 605 |
| BstDSI | CCRYGG | 1 cut(s) | 456 |
| BstF5I | GGATG | 3 cut(s) | 251, 291, 295 |
| BstFNI | CGCG | 1 cut(s) | 283 |
| BstHHI | GCGC | 1 cut(s) | 251 |
| BstKTI | GATC | 4 cut(s) | 57, 418, 422, 427 |
| BstMAI | GTCTC | 1 cut(s) | 223 |
| BstMBI | GATC | 4 cut(s) | 54, 415, 419, 424 |
| BstSFI | CTRYAG | 1 cut(s) | 486 |
| BstUI | CGCG | 1 cut(s) | 283 |
| BstV1I | GCAGC | 2 cut(s) | 348, 500 |
| Bsu15I | ATCGAT | 1 cut(s) | 418 |
| BsuI | GTATCC | 1 cut(s) | 454 |
| BsuRI | GGCC | 3 cut(s) | 350, 478, 525 |
| BsuTUI | ATCGAT | 1 cut(s) | 418 |
| BtgI | CCRYGG | 1 cut(s) | 456 |
| BtsCI | GGATG | 3 cut(s) | 251, 291, 295 |
| BtsI | GCAGTG | 1 cut(s) | 643 |
| BtsIMutI | CAGTG | 2 cut(s) | 643, 650 |
| Cac8I | GCNNGC | 1 cut(s) | 605 |
| CfoI | GCGC | 1 cut(s) | 251 |
| Cfr13I | GGNCC | 1 cut(s) | 523 |
| ClaI | ATCGAT | 1 cut(s) | 418 |
| CseI | GACGC | 1 cut(s) | 95 |
| Csp6I | GTAC | 1 cut(s) | 32 |
| CviAII | CATG | 5 cut(s) | 265, 358, 457, 609, 626 |
| CviJI | RGCY | 8 cut(s) | 197, 232, 350, 478, 491, 525, 596, 649 |
| CviKI_1 | RGCY | 8 cut(s) | 197, 232, 350, 478, 491, 525, 596, 649 |
| CviQI | GTAC | 1 cut(s) | 32 |
| DpnI | GATC | 4 cut(s) | 56, 417, 421, 426 |
| DpnII | GATC | 4 cut(s) | 54, 415, 419, 424 |
| EaeI | YGGCCR | 1 cut(s) | 348 |
| Ecl136II | GAGCTC | 1 cut(s) | 197 |
| Eco130I | CCWWGG | 1 cut(s) | 456 |
| Eco147I | AGGCCT | 1 cut(s) | 478 |
| Eco24I | GRGCYC | 1 cut(s) | 199 |
| Eco31I | GGTCTC | 1 cut(s) | 223 |
| Eco53kI | GAGCTC | 1 cut(s) | 197 |
| EcoICRI | GAGCTC | 1 cut(s) | 197 |
| EcoT14I | CCWWGG | 1 cut(s) | 456 |
| EcoT38I | GRGCYC | 1 cut(s) | 199 |
| ErhI | CCWWGG | 1 cut(s) | 456 |
| FaeI | CATG | 5 cut(s) | 268, 361, 460, 612, 629 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 693 |
| FaqI | GGGAC | 4 cut(s) | 55, 143, 152, 278 |
| FatI | CATG | 5 cut(s) | 264, 357, 456, 608, 625 |
| FauNDI | CATATG | 1 cut(s) | 466 |
| FbaI | TGATCA | 1 cut(s) | 54 |
| Fnu4HI | GCNGC | 2 cut(s) | 362, 489 |
| FokI | GGATG | 3 cut(s) | 238, 298, 302 |
| FriOI | GRGCYC | 1 cut(s) | 199 |
| Fsp4HI | GCNGC | 2 cut(s) | 362, 489 |
| FspBI | CTAG | 1 cut(s) | 42 |
| GlaI | GCGC | 1 cut(s) | 250 |
| GluI | GCNGC | 2 cut(s) | 362, 489 |
| HaeIII | GGCC | 3 cut(s) | 350, 478, 525 |
| HgaI | GACGC | 1 cut(s) | 95 |
| HhaI | GCGC | 1 cut(s) | 251 |
| Hin1II | CATG | 5 cut(s) | 268, 361, 460, 612, 629 |
| Hin6I | GCGC | 1 cut(s) | 249 |
| HinP1I | GCGC | 1 cut(s) | 249 |
| HinfI | GANTC | 5 cut(s) | 140, 433, 452, 533, 551 |
| HphI | GGTGA | 1 cut(s) | 596 |
| Hpy166II | GTNNAC | 3 cut(s) | 32, 376, 614 |
| Hpy188I | TCNGA | 6 cut(s) | 145, 241, 424, 429, 438, 538 |
| Hpy188III | TCNNGA | 1 cut(s) | 200 |
| Hpy8I | GTNNAC | 3 cut(s) | 32, 376, 614 |
| HpyCH4III | ACNGT | 2 cut(s) | 259, 313 |
| HpyCH4IV | ACGT | 2 cut(s) | 385, 588 |
| HpyCH4V | TGCA | 2 cut(s) | 488, 636 |
| HpySE526I | ACGT | 2 cut(s) | 385, 588 |
| Hsp92II | CATG | 5 cut(s) | 268, 361, 460, 612, 629 |
| HspAI | GCGC | 1 cut(s) | 249 |
| Ksp22I | TGATCA | 1 cut(s) | 54 |
| Kzo9I | GATC | 4 cut(s) | 54, 415, 419, 424 |
| LmnI | GCTCC | 1 cut(s) | 246 |
| LpnPI | CCDG | 6 cut(s) | 309, 320, 498, 617, 656, 670 |
| Lsp1109I | GCAGC | 2 cut(s) | 348, 500 |
| LweI | GCATC | 1 cut(s) | 260 |
| MaeI | CTAG | 1 cut(s) | 42 |
| MaeII | ACGT | 2 cut(s) | 385, 588 |
| MaeIII | GTNAC | 4 cut(s) | 388, 442, 584, 659 |
| MalI | GATC | 4 cut(s) | 56, 417, 421, 426 |
| MboI | GATC | 4 cut(s) | 54, 415, 419, 424 |
| MboII | GAAGA | 5 cut(s) | 158, 311, 551, 710, 713 |
| MhlI | GDGCHC | 3 cut(s) | 199, 264, 342 |
| MlsI | TGGCCA | 1 cut(s) | 350 |
| MluCI | AATT | 3 cut(s) | 104, 344, 631 |
| MluNI | TGGCCA | 1 cut(s) | 350 |
| MlyI | GAGTC | 2 cut(s) | 134, 560 |
| MnlI | CCTC | 6 cut(s) | 208, 265, 405, 432, 463, 574 |
| Mox20I | TGGCCA | 1 cut(s) | 350 |
| MscI | TGGCCA | 1 cut(s) | 350 |
| MseI | TTAA | 2 cut(s) | 191, 504 |
| Msp20I | TGGCCA | 1 cut(s) | 350 |
| MvnI | CGCG | 1 cut(s) | 283 |
| NcoI | CCATGG | 1 cut(s) | 456 |
| NdeI | CATATG | 1 cut(s) | 466 |
| NdeII | GATC | 4 cut(s) | 54, 415, 419, 424 |
| NlaIII | CATG | 5 cut(s) | 268, 361, 460, 612, 629 |
| NmeAIII | GCCGAG | 1 cut(s) | 253 |
| NmuCI | GTSAC | 2 cut(s) | 584, 659 |
| PceI | AGGCCT | 1 cut(s) | 478 |
| PfeI | GAWTC | 3 cut(s) | 433, 452, 533 |
| PkrI | GCNGC | 2 cut(s) | 363, 490 |
| PleI | GAGTC | 2 cut(s) | 134, 559 |
| PpsI | GAGTC | 2 cut(s) | 134, 559 |
| Psp124BI | GAGCTC | 1 cut(s) | 199 |
| PspPI | GGNCC | 1 cut(s) | 523 |
| PstI | CTGCAG | 1 cut(s) | 490 |
| RsaI | GTAC | 1 cut(s) | 33 |
| RsaNI | GTAC | 1 cut(s) | 32 |
| SacI | GAGCTC | 1 cut(s) | 199 |
| SaqAI | TTAA | 2 cut(s) | 191, 504 |
| SatI | GCNGC | 2 cut(s) | 362, 489 |
| Sau3AI | GATC | 4 cut(s) | 54, 415, 419, 424 |
| Sau96I | GGNCC | 1 cut(s) | 523 |
| SchI | GAGTC | 2 cut(s) | 134, 560 |
| SduI | GDGCHC | 3 cut(s) | 199, 264, 342 |
| SfaNI | GCATC | 1 cut(s) | 260 |
| SfcI | CTRYAG | 1 cut(s) | 486 |
| SmlI | CTYRAG | 1 cut(s) | 198 |
| SmoI | CTYRAG | 1 cut(s) | 198 |
| Sse9I | AATT | 3 cut(s) | 104, 344, 631 |
| SseBI | AGGCCT | 1 cut(s) | 478 |
| SsiI | CCGC | 2 cut(s) | 132, 283 |
| SspI | AATATT | 1 cut(s) | 64 |
| SspMI | CTAG | 1 cut(s) | 42 |
| SstI | GAGCTC | 1 cut(s) | 199 |
| StuI | AGGCCT | 1 cut(s) | 478 |
| StyI | CCWWGG | 1 cut(s) | 456 |
| TaaI | ACNGT | 2 cut(s) | 259, 313 |
| TaiI | ACGT | 2 cut(s) | 388, 591 |
| TaqI | TCGA | 2 cut(s) | 342, 418 |
| TasI | AATT | 3 cut(s) | 104, 344, 631 |
| TfiI | GAWTC | 3 cut(s) | 433, 452, 533 |
| Tru1I | TTAA | 2 cut(s) | 191, 504 |
| Tru9I | TTAA | 2 cut(s) | 191, 504 |
| TscAI | CASTG | 2 cut(s) | 643, 650 |
| TseFI | GTSAC | 2 cut(s) | 584, 659 |
| TseI | GCWGC | 2 cut(s) | 361, 488 |
| Tsp45I | GTSAC | 2 cut(s) | 584, 659 |
| TspDTI | ATGAA | 4 cut(s) | 318, 465, 490, 642 |
| TspRI | CASTG | 2 cut(s) | 643, 650 |
| XapI | RAATTY | 1 cut(s) | 104 |
| XspI | CTAG | 1 cut(s) | 42 |