FvH4_6g51870

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000056
Physical Location & Seq
Forward (+)
38387215 .. 38388709
1495 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g51870.t1

Sequence Viewer

Length: 636 bp
ATGGAGGGATATTACCGCTACGTTCACCAGGCGCTGCAAAAGAGGGCAACCAAGCTCATAGGCTCTTGGGAGGAGGACAAGCTTTTCATCAGAATCAAGCTTTACAAGTCAACCAGATTGAGCAGGGATCCACTTGTTTTTGATGACCATAAAATAGCCCAAAGAGATTTTACGGTCAAGTTTTCTCATTTCAAGCAGCAATTATCCAACAACTGCGGCAGTGATATGGAGTATGAAGCACTCATCTCCGAGCATCTGAAGAGTATGGGTGTGCCCCAACATGAACATTCTGCTATTGCTCAAAGGGTGTTCCTGCTGGTTGAGGCGGCTGTCACCCCTGTGGACGCTGTTGTGGTTGGTGTGTGGGACACCTTTTACCCACCTCTATATGTACCACATATCCCCGATTTCGTTCCTGCGAGCAGATCATCGATCCGGGCTCTGGAGCAAGTGACACTCGATAGTGAGACTATCGCATGCACGCCCTCATGTGCTATTTGTTTGGAGGATTTTGAACAAGAACCTGTTACTCCGTTGCCCTGCCGACACCATTTTCATCTACATTGCATTGTTCAGTACTTGGAGGTGAACCACATGTGTCCCCTCTGCCGATACTCAATGCCGACGCAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

212

Amino Acids

24.33

Weight (kDa)

6.04

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 163 - 204 3e-12 Ring finger domain
zf-RING_11 PF17123 164 - 190 1.2e-07 RING-like zinc finger
zf-C3HC4_2 PF13923 164 - 203 2.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 164 - 204 3.1e-06 RING-H2 zinc finger domain
zf-C3HC4 PF00097 164 - 203 1.2e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000056)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74620
fragaria_vesca FvH4_1g17310 FvH4_1g21860 FvH4_2g00301 FvH4_2g00500 FvH4_2g00510 FvH4_2g01040 FvH4_2g01040 FvH4_2g01040 FvH4_2g01511 FvH4_2g01521 FvH4_2g05410 FvH4_2g15860 FvH4_2g16170 FvH4_2g20442 FvH4_3g22600 FvH4_4g03111 FvH4_4g31810 FvH4_4g31840 FvH4_4g33571 FvH4_4g33580 FvH4_4g33600 FvH4_4g33620 FvH4_4g33620 FvH4_5g04820 FvH4_5g04820 FvH4_5g04821 FvH4_5g04821 FvH4_5g04830 FvH4_5g04833 FvH4_5g04833 FvH4_5g04834 FvH4_5g05562 FvH4_5g06150 FvH4_5g07970 FvH4_5g09910 FvH4_5g11280 FvH4_5g12010 FvH4_6g06910 FvH4_6g11580 FvH4_6g15110 FvH4_6g24322 FvH4_6g31360 FvH4_6g34080 FvH4_6g44500 FvH4_6g47060 FvH4_6g50581 FvH4_6g50641 FvH4_6g50650 FvH4_6g50650 FvH4_6g50981 FvH4_6g51090 FvH4_6g51180 FvH4_6g51730 FvH4_6g51740 FvH4_6g51870 FvH4_6g52331 FvH4_6g52340 FvH4_6g52350 FvH4_6g52530 FvH4_6g52530
malus_domestica MD14G1195700.v1.1
prunus_persica Prupe.6G084800_v2.0.a1 Prupe.7G084100_v2.0.a1 Prupe.8G048800_v2.0.a1
pyrus_communis pycom17g01390
rosa_chinensis RchiOBHm_Chr0c11g0499451 RchiOBHm_Chr1g0314791 RchiOBHm_Chr1g0340221 RchiOBHm_Chr1g0362121 RchiOBHm_Chr1g0362131 RchiOBHm_Chr2g0107491 RchiOBHm_Chr2g0127721 RchiOBHm_Chr2g0143901 RchiOBHm_Chr2g0161531 RchiOBHm_Chr2g0169511 RchiOBHm_Chr2g0171151 RchiOBHm_Chr2g0171221 RchiOBHm_Chr2g0171251 RchiOBHm_Chr2g0171261 RchiOBHm_Chr2g0171341 RchiOBHm_Chr2g0171561 RchiOBHm_Chr2g0171591 RchiOBHm_Chr2g0171601 RchiOBHm_Chr2g0172411 RchiOBHm_Chr2g0172441 RchiOBHm_Chr2g0172771 RchiOBHm_Chr2g0172881 RchiOBHm_Chr2g0173201 RchiOBHm_Chr2g0173211 RchiOBHm_Chr2g0173941 RchiOBHm_Chr2g0173951 RchiOBHm_Chr2g0175281 RchiOBHm_Chr3g0463081 RchiOBHm_Chr3g0482941 RchiOBHm_Chr3g0486241 RchiOBHm_Chr3g0488151 RchiOBHm_Chr3g0488191 RchiOBHm_Chr3g0488271 RchiOBHm_Chr3g0488351 RchiOBHm_Chr3g0488411 RchiOBHm_Chr3g0488491 RchiOBHm_Chr4g0410881 RchiOBHm_Chr4g0440431 RchiOBHm_Chr4g0440501 RchiOBHm_Chr6g0269041 RchiOBHm_Chr7g0194201 RchiOBHm_Chr7g0194301 RchiOBHm_Chr7g0194311 RchiOBHm_Chr7g0194391 RchiOBHm_Chr7g0194401 RchiOBHm_Chr7g0236181
rosa_laevigata RLG00000004203 RLG00000006176 RLG00000022170 RLG00000022198 RLG00000022237 RLG00000022911
rosa_multiflora Rmu_co8086672.1_g000001 Rmu_co8091660.1_g000001 Rmu_co8325445.1_g000001 Rmu_co8482323.1_g000001 Rmu_sc0000239.1_g000034 Rmu_sc0000467.1_g000001 Rmu_sc0000766.1_g000038 Rmu_sc0000789.1_g000004 Rmu_sc0000831.1_g000055 Rmu_sc0000831.1_g000056 Rmu_sc0000831.1_g000057 Rmu_sc0000831.1_g000058 Rmu_sc0000831.1_g000059 Rmu_sc0000831.1_g000060 Rmu_sc0000901.1_g000011 Rmu_sc0000901.1_g000029 Rmu_sc0001513.1_g000006 Rmu_sc0001513.1_g000011 Rmu_sc0001576.1_g000034 Rmu_sc0001685.1_g000062 Rmu_sc0002784.1_g000036 Rmu_sc0003188.1_g000026 Rmu_sc0003188.1_g000027 Rmu_sc0003314.1_g000017 Rmu_sc0003548.1_g000019 Rmu_sc0003624.1_g000018 Rmu_sc0005193.1_g000015 Rmu_sc0006328.1_g000001 Rmu_sc0006328.1_g000005 Rmu_sc0006806.1_g000014 Rmu_sc0006806.1_g000028 Rmu_sc0007320.1_g000005 Rmu_sc0007493.1_g000003 Rmu_sc0007646.1_g000011 Rmu_sc0007648.1_g000001 Rmu_sc0007661.1_g000007 Rmu_sc0007684.1_g000008 Rmu_sc0007799.1_g000007 Rmu_sc0007841.1_g000004 Rmu_sc0008051.1_g000003 Rmu_sc0008124.1_g000008 Rmu_sc0009104.1_g000008 Rmu_sc0009433.1_g000012 Rmu_sc0009883.1_g000011 Rmu_sc0010744.1_g000003 Rmu_sc0011052.1_g000003 Rmu_sc0012403.1_g000008 Rmu_sc0013831.1_g000001 Rmu_sc0016754.1_g000004 Rmu_sc0022238.1_g000002 Rmu_sc0023157.1_g000001 Rmu_sc0023894.1_g000001 Rmu_sc0028130.1_g000001 Rmu_sc0040543.1_g000001 Rmu_ssc0000252.1_g000003 Rmu_ssc0000252.1_g000004 Rmu_ssc0000261.1_g000030 Rmu_ssc0000326.1_g000007
rosa_roxburghii Rroxscaffold_157G00438560 Rroxscaffold_2G00078410 Rroxscaffold_2G00078970 Rroxscaffold_2G00079030 Rroxscaffold_2G00079120 Rroxscaffold_2G00079340 Rroxscaffold_2G00079720 Rroxscaffold_2G00079870 Rroxscaffold_2G00079910 Rroxscaffold_2G00080570 Rroxscaffold_2G00080720 Rroxscaffold_2G00080770 Rroxscaffold_2G00080780 Rroxscaffold_2G00080850 Rroxscaffold_2G00080910 Rroxscaffold_2G00116750 Rroxscaffold_3G00261370 Rroxscaffold_3G00261500 Rroxscaffold_3G00266200 Rroxscaffold_4G00305290 Rroxscaffold_4G00311110 Rroxscaffold_4G00327720 Rroxscaffold_5G00381290 Rroxscaffold_5G00381320 Rroxscaffold_6G00394220 Rroxscaffold_6G00416970
rosa_rugosa Rorug01G0164800.1 Rorug01G0164900 Rorug01G0293800 Rorug01G0293900 Rorug01G0294200 Rorug02G0144400 Rorug02G0271300 Rorug02G0271400 Rorug02G0271400 Rorug02G0271500 Rorug02G0384900 Rorug02G0384900 Rorug02G0385000 Rorug02G0385100 Rorug02G0553000 Rorug02G0553100 Rorug02G0555000 Rorug02G0560000 Rorug02G0560100 Rorug02G0561500 Rorug02G0566500 Rorug02G0568800 Rorug02G0569000 Rorug02G0569400 Rorug02G0569700 Rorug02G0575200 Rorug02G0582300 Rorug03G0061300 Rorug03G0126300 Rorug03G0202400 Rorug03G0224200 Rorug03G0238200 Rorug03G0238300 Rorug03G0238400 Rorug03G0255100 Rorug03G0255100 Rorug04G0326000 Rorug04G0326200 Rorug04G0326300 Rorug06G0124000 Rorug07G0015300 Rorug07G0015700 Rorug07G0015700 Rorug07G0265000
rosa_samantha Rh1AG304100 Rh1AG304500 Rh1AG304600 Rh1CG166600 Rh1CG285500 Rh1DG218600 Rh2AG323600 Rh2AG611800 Rh2AG626100 Rh2AG626400 Rh2AG627700 Rh2AG629700 Rh2AG634400 Rh2AG634500 Rh2AG634800 Rh2AG652800 Rh2BG206800 Rh2BG636300 Rh2BG636400 Rh2BG637300 Rh2BG637400 Rh2BG642600 Rh2BG648000 Rh2BG648300 Rh2BG652700 Rh2BG664000 Rh2CG311300 Rh2CG604800 Rh2CG605400 Rh2CG606300 Rh2CG606800 Rh2CG623800 Rh2CG628700 Rh2DG201600 Rh2DG351100 Rh2DG648000 Rh2DG648100 Rh2DG648900 Rh2DG649000 Rh2DG649100 Rh2DG651000 Rh2DG654900 Rh2DG655100 Rh2DG655200 Rh2DG656700 Rh2DG656900 Rh2DG657100 Rh2DG662700 Rh2DG662900 Rh2DG666300 Rh2DG667000 Rh2DG670700 Rh2DG672100 Rh2DG673400 Rh2DG673600 Rh2DG675200 Rh2DG675300 Rh2DG675600 Rh2DG679000 Rh2DG679100 Rh3AG048000 Rh3AG048100 Rh3AG106800 Rh3AG119500 Rh3AG251300 Rh3AG285800 Rh3AG286700 Rh3AG287300 Rh3AG287400 Rh3CG049400 Rh3CG321600 Rh3DG320300 Rh4AG377300 Rh4AG377700 Rh4AG377900 Rh4DG159200 Rh7AG064400 Rh7AG097600 Rh7AG141500 Rh7AG142100 Rh7AG142200 Rh7AG142300 Rh7AG142600 Rh7AG142800 Rh7AG417800 Rh7AG424000 Rh7CG147100 Rh7DG143000 Rh7DG143900 Rh7DG144400 Rh7DG144700
rosa_wichuraiana Rw0G019520 Rw1G014950 Rw1G027080 Rw2G015390 Rw2G035650 Rw2G046060 Rw2G050780 Rw2G050810 Rw2G051810 Rw2G051850 Rw2G051940 Rw2G051990 Rw2G052000 Rw2G052130 Rw2G052140 Rw2G052530 Rw2G052760 Rw2G052830 Rw2G053180 Rw2G053330 Rw2G053630 Rw3G010110 Rw3G024300 Rw3G025400 Rw3G025450 Rw4G032320 Rw4G032440 Rw6G037400 Rw7G012180 Rw7G012220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 16, 216, 326
AclWI GGATC 3 cut(s) 122, 135, 427
AcuI CTGAAG 1 cut(s) 278
AfaI GTAC 2 cut(s) 393, 578
AfiI CCNNNNNNNGG 1 cut(s) 442
AflIII ACRYGT 1 cut(s) 594
AgsI TTSAA 2 cut(s) 193, 515
AjnI CCWGG 1 cut(s) 27
AleI CACNNNNGTG 1 cut(s) 338
AluBI AGCT 3 cut(s) 55, 82, 100
AluI AGCT 3 cut(s) 55, 82, 100
Alw26I GTCTC 1 cut(s) 461
AlwI GGATC 3 cut(s) 122, 135, 427
AlwNI CAGNNNCTG 1 cut(s) 34
ApeKI GCWGC 2 cut(s) 34, 196
AspLEI GCGC 1 cut(s) 34
AsuC2I CCSGG 1 cut(s) 437
AsuHPI GGTGA 3 cut(s) 17, 325, 598
BaeGI GKGCMC 1 cut(s) 276
BamHI GGATCC 1 cut(s) 127
BanII GRGCYC 1 cut(s) 442
BbvI GCAGC 2 cut(s) 21, 208
BciT130I CCWGG 1 cut(s) 29
BcnI CCSGG 1 cut(s) 437
BcoDI GTCTC 1 cut(s) 461
BfoI RGCGCY 1 cut(s) 35
BisI GCNGC 4 cut(s) 35, 197, 217, 327
BlsI GCNGC 4 cut(s) 36, 198, 218, 328
BmcAI AGTACT 1 cut(s) 578
Bme1390I CCNGG 2 cut(s) 29, 437
BmiI GGNNCC 1 cut(s) 129
BmrFI CCNGG 2 cut(s) 29, 437
BmsI GCATC 1 cut(s) 262
BpmI CTGGAG 1 cut(s) 464
BpuMI CCSGG 1 cut(s) 437
Bsa29I ATCGAT 1 cut(s) 431
Bsc4I CCNNNNNNNGG 1 cut(s) 442
Bse3DI GCAATG 1 cut(s) 562
BseBI CCWGG 1 cut(s) 29
BseCI ATCGAT 1 cut(s) 431
BseLI CCNNNNNNNGG 1 cut(s) 442
BseMI GCAATG 1 cut(s) 562
BseRI GAGGAG 1 cut(s) 86
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 2 cut(s) 21, 208
BshVI ATCGAT 1 cut(s) 431
BsiSI CCGG 1 cut(s) 436
BslFI GGGAC 2 cut(s) 380, 585
BslI CCNNNNNNNGG 1 cut(s) 442
BsmAI GTCTC 1 cut(s) 461
BsmFI GGGAC 2 cut(s) 380, 585
Bsp1286I GDGCHC 2 cut(s) 276, 442
Bsp143I GATC 3 cut(s) 127, 425, 432
BspACI CCGC 3 cut(s) 16, 216, 326
BspDI ATCGAT 1 cut(s) 431
BspLI GGNNCC 1 cut(s) 129
BspPI GGATC 3 cut(s) 122, 135, 427
BsrDI GCAATG 1 cut(s) 562
BssMI GATC 3 cut(s) 127, 425, 432
Bst2UI CCWGG 1 cut(s) 29
Bst4CI ACNGT 1 cut(s) 175
Bst6I CTCTTC 1 cut(s) 254
BstC8I GCNNGC 3 cut(s) 421, 478, 482
BstH2I RGCGCY 1 cut(s) 35
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 3 cut(s) 130, 428, 435
BstMAI GTCTC 1 cut(s) 461
BstMBI GATC 3 cut(s) 127, 425, 432
BstNI CCWGG 1 cut(s) 29
BstNSI RCATGY 2 cut(s) 480, 598
BstSCI CCNGG 2 cut(s) 27, 435
BstSLI GKGCMC 1 cut(s) 276
BstV1I GCAGC 2 cut(s) 21, 208
BstX2I RGATCY 1 cut(s) 127
BstYI RGATCY 1 cut(s) 127
Bsu15I ATCGAT 1 cut(s) 431
BsuTUI ATCGAT 1 cut(s) 431
BtsI GCAGTG 1 cut(s) 226
BtsIMutI CAGTG 1 cut(s) 226
Cac8I GCNNGC 3 cut(s) 421, 478, 482
CaiI CAGNNNCTG 1 cut(s) 34
CfoI GCGC 1 cut(s) 34
ClaI ATCGAT 1 cut(s) 431
CseI GACGC 1 cut(s) 353
Csp6I GTAC 2 cut(s) 392, 577
CviAII CATG 4 cut(s) 281, 477, 489, 595
CviJI RGCY 7 cut(s) 55, 63, 82, 100, 158, 329, 440
CviKI_1 RGCY 7 cut(s) 55, 63, 82, 100, 158, 329, 440
CviQI GTAC 2 cut(s) 392, 577
DpnI GATC 3 cut(s) 129, 427, 434
DpnII GATC 3 cut(s) 127, 425, 432
Eam1104I CTCTTC 1 cut(s) 254
EarI CTCTTC 1 cut(s) 254
Eco24I GRGCYC 1 cut(s) 442
Eco57I CTGAAG 1 cut(s) 278
EcoRII CCWGG 1 cut(s) 27
EcoT38I GRGCYC 1 cut(s) 442
FaeI CATG 4 cut(s) 284, 480, 492, 598
FaqI GGGAC 2 cut(s) 380, 585
FatI CATG 4 cut(s) 280, 476, 488, 594
Fnu4HI GCNGC 4 cut(s) 35, 197, 217, 327
FriOI GRGCYC 1 cut(s) 442
Fsp4HI GCNGC 4 cut(s) 35, 197, 217, 327
GlaI GCGC 1 cut(s) 33
GluI GCNGC 4 cut(s) 35, 197, 217, 327
GsuI CTGGAG 1 cut(s) 464
HaeII RGCGCY 1 cut(s) 35
HapII CCGG 1 cut(s) 436
HgaI GACGC 1 cut(s) 353
HhaI GCGC 1 cut(s) 34
Hin1II CATG 4 cut(s) 284, 480, 492, 598
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HincII GTYRAC 1 cut(s) 111
HindII GTYRAC 1 cut(s) 111
HindIII AAGCTT 2 cut(s) 80, 98
HinfI GANTC 1 cut(s) 93
HpaII CCGG 1 cut(s) 436
HphI GGTGA 3 cut(s) 17, 325, 598
Hpy166II GTNNAC 4 cut(s) 25, 111, 343, 589
Hpy188I TCNGA 3 cut(s) 92, 250, 258
Hpy188III TCNNGA 1 cut(s) 443
Hpy8I GTNNAC 4 cut(s) 25, 111, 343, 589
Hpy99I CGWCG 1 cut(s) 628
HpyCH4III ACNGT 1 cut(s) 175
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 3 cut(s) 37, 480, 567
HpySE526I ACGT 1 cut(s) 21
Hsp92II CATG 4 cut(s) 284, 480, 492, 598
HspAI GCGC 1 cut(s) 32
Kzo9I GATC 3 cut(s) 127, 425, 432
LmnI GCTCC 1 cut(s) 445
Lsp1109I GCAGC 2 cut(s) 21, 208
LweI GCATC 1 cut(s) 262
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 3 cut(s) 331, 451, 526
MalI GATC 3 cut(s) 129, 427, 434
MboI GATC 3 cut(s) 127, 425, 432
MboII GAAGA 1 cut(s) 271
MflI RGATCY 1 cut(s) 127
MhlI GDGCHC 2 cut(s) 276, 442
MluCI AATT 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 231
MnlI CCTC 9 cut(s) 36, 64, 67, 316, 393, 496, 499, 577, 614
MseI TTAA 1 cut(s) 634
MslI CAYNNNNRTG 1 cut(s) 338
MspI CCGG 1 cut(s) 436
MspR9I CCNGG 2 cut(s) 29, 437
MvaI CCWGG 1 cut(s) 29
NciI CCSGG 1 cut(s) 437
NdeII GATC 3 cut(s) 127, 425, 432
NlaIII CATG 4 cut(s) 284, 480, 492, 598
NlaIV GGNNCC 1 cut(s) 129
NmuCI GTSAC 2 cut(s) 331, 451
NspI RCATGY 2 cut(s) 480, 598
OliI CACNNNNGTG 1 cut(s) 338
PaeI GCATGC 1 cut(s) 480
PciI ACATGT 1 cut(s) 594
PfeI GAWTC 1 cut(s) 93
PkrI GCNGC 4 cut(s) 36, 198, 218, 328
PscI ACATGT 1 cut(s) 594
Psp6I CCWGG 1 cut(s) 27
PspGI CCWGG 1 cut(s) 27
PspN4I GGNNCC 1 cut(s) 129
PstNI CAGNNNCTG 1 cut(s) 34
PsuI RGATCY 1 cut(s) 127
RsaI GTAC 2 cut(s) 393, 578
RsaNI GTAC 2 cut(s) 392, 577
RseI CAYNNNNRTG 1 cut(s) 338
SaqAI TTAA 1 cut(s) 634
SatI GCNGC 4 cut(s) 35, 197, 217, 327
Sau3AI GATC 3 cut(s) 127, 425, 432
ScaI AGTACT 1 cut(s) 578
ScrFI CCNGG 2 cut(s) 29, 437
SduI GDGCHC 2 cut(s) 276, 442
SetI ASST 8 cut(s) 24, 57, 84, 102, 374, 385, 526, 588
SfaNI GCATC 1 cut(s) 262
SmiMI CAYNNNNRTG 1 cut(s) 338
SphI GCATGC 1 cut(s) 480
Sse9I AATT 1 cut(s) 200
SsiI CCGC 3 cut(s) 16, 216, 326
StyD4I CCNGG 2 cut(s) 27, 435
TaaI ACNGT 1 cut(s) 175
TaiI ACGT 1 cut(s) 24
TaqI TCGA 2 cut(s) 431, 459
TasI AATT 1 cut(s) 200
TatI WGTACW 1 cut(s) 576
TauI GCSGC 2 cut(s) 219, 329
TfiI GAWTC 1 cut(s) 93
Tru1I TTAA 1 cut(s) 634
Tru9I TTAA 1 cut(s) 634
TscAI CASTG 1 cut(s) 226
TseFI GTSAC 2 cut(s) 331, 451
TseI GCWGC 2 cut(s) 34, 196
Tsp45I GTSAC 2 cut(s) 331, 451
TspDTI ATGAA 4 cut(s) 76, 249, 297, 545
TspGWI ACGGA 1 cut(s) 522
TspRI CASTG 1 cut(s) 226
XceI RCATGY 2 cut(s) 480, 598
ZrmI AGTACT 1 cut(s) 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.