Rorug04G0326200

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000056
Physical Location & Seq
Reverse (-)
50107452 .. 50118647
11196 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0326200.1

Sequence Viewer

Length: 3495 bp
ATGCTCTTTCCTATGAAGGCCAAAGGATGCTATTATGAAAATTGGAAGCTTGGTTGTGTAACTGGATCTAACTCAGAGTTGGAATTTGCAGACTTCTCAAAATCAAGTTTGTTCAAGGTTGAAATGCAAACAAGGAAAAAAAGTTCTGGAAGAACTGCTTCTAGAGAGCATACCAGTCCCAAGGTTACAAGAGCTCAGAAGAAAACACATGAAAATGTCAAAGTATTGGATACCAAAGTTACAGATATGATTACATCTTCATCCAGAAAGCAGAGACTCGGTGGGACTCATAAGAAGAATGAGGAGCCTGTTACTGCAAGCACTTTAAGTACAAATTATGACTTGCTGCTTAATGAGACTGCCGGGGCTTGTTTGGGGCATGATGCTTTAAGTGATGATTGCAAAGAGAATAAGGGTTGTGAGGAGGGTGCTGCTGATTGCGTTCTGGGAACTATATTTTCTCCTGCCTTTCACATACCGAAACATGCTGGTGGAGAATCTGCCAATGGAGTTGATTTTGTTAAATTCTTTCAAAATGGAGATCACAATTTCCATAAAGAGATGGAAATAAAGTACTCACCCGATGATGCACACCATGGTTATGTTGGCCAAGAGACATCGGAGTCCACTGTTCGAAACGAGAACAACTACTGTGAGAACTCATTATCTTCTGTAACTATAACATGTCCTGTTGTGGAGGCAACCAATATTTCTTCTGAAGTCTCAGCCATATATCTTGCCATGAAAAATTCCAAGTTGGAATGTTTAGATGAGCATGGTCAGGAACCTATGTCAACTGATTTGTACGTGGATGATGATGAGAGCGAGGAATTTGATGACTTTGATCCTTACTTATTTATCAAGAACTTACCTGACTTGTCCTCAGTTGTCCCCACTTTTCGGCCGTTGCTGCTGCCTAAGCAGACTCGAAGTTGTCCTCCTACAAGTCTTGTTTTGGACTTGGATGAAACTCTGGTACATTCCACACTAGAACCTTGTGATGATGCAGACTTCACATTTCCCGTAAATTTTAACCTACAAGAGCATACAGTATATGTTCGATGCCGTCCTCATCTCAGAGATTTTCTGGACAGAGTTTCGCTTCTTTTCGAGATCATTATCTTCACAGCTAGTCAAAGTATCTATGCAGAGCAGCTTCTAAATGTGCTGGATCCGAAGAGGAAGATTTTTCGCCATCGTGTTTTTCGTGATTCCTGTGTTTTTGTGGAGGGGAATTATCTCAAAGATTTGTCGGTTCTTGGCCGTGATTTGGCACGTGTTGTCATAGTCGACAACTCTCCACAGGCATTTGGGTTCCAAGTGGACAATGGAATACCGATTGAGAGCTGGTTTGATGATCGTTCTGATACAGAATTACTTTCATTACTTCCTTTTTTGGAAAGCTTGGTTGGAGTAGAAGATGTTCGACCGCTAATTGCAAACAAATTCAACCTCCGGGAGAAAATAGCTACTGCTGATTATCCACTAAACTCAAATAGAGGTGATTCTTTAGTGCCAATTGTTCTGTTTGTTGTGAAGTTACGCGGGTCAGATACAAGGGAGACTGGTAAACTGCTGACAGATAATCAGCTTAGAAATAAGGTTCTTGATAATTTAAAGGTTGTGGCTCAAAAACTGAAGGAGGCGGAGATCACCGAGCAGGATTCGCTTCTTCTGACGAGGAACCTGCTTCGCATCGCTATATTCAATATCAGTTACATCAGAGGCCTTTTTCCGGAGAAGTATTTCAATGATAAATCTGTTCCTGCTTTAGAGATGAAGATTAAAAAGCTAATGCCAATGGATGCAGAGTCTCGCAGACTTATTGATTGGATGGAGAAAGGTGTATATGACGCCTTGCAGAAGAAGTACCTGAAAACGCTTTTGTTCTGTGTGTGTGAAGAGGTTGAAGGGCCGATGATAGAGGAATACGCATTTTCATTCAGTTACTCAAATTCCGAGAGCCAGGAGGTTTCAATGAATATCAGTCGCTCTGGAAACAAGAAGCATGGTGGAACATTCAAGTGTGACTCCACAGCAGAAATTACCCCCAACCAGATGAGAAGTTCTGCTTGTAAAATGGTCCGCACACTGGTTCAACTGATGAGAACTCTGGATAGGATGCCTGAAGAGCGCACCATACTGATGAAACTCCTATATTATGATGATGTGACGCCAGTAGATTATGAGCCTCCATTCTTCAGATGTTGCACAGAAGAAGAAACTCATAACCCATGGACAAAGAATCCTTTGAAACTGGAGGTCGGGAATGTGAACAGCAAGCATCTTGTCTTAGCTCTCAAGGTAAAGAGCGTGCTTGATCCCTGTGAGGATGAAAATGATGATAATCAAGATGATGAAGTGAGCTTAGGAGCTGACTCCATGCAAAGGGATGACTCTTCTGAAACTGACAGTGAGGTTAACCAATCACAAGAGGATCGATATATAGTTGCCCCCATAGCTAAGCGACAACCACAGGAGGATAACAGCATACCTCAGGAAGTTAACAGCATGGATGATGAAGCTACAGATGATACTCAGGATCCAGAGGAAGATGAACAGCAATTTTCTAGGATAAAGGACTGGATCAGTAGCAGCCATCTTGACAATGTTGAAGTTACTGATGTTCTCTCGAATTTCCCAGACATCTCAGTGGTTCTGACTGAAGAAATTATGGATAGGCTTGTTGCTGAAGGTGTTCTATCAAAAACTGGAGGGGACAGTTACACAATTAACAGGAAGAAGAAGTCTGATCATGAATTTATCAAGGTAAAAAAAGAAATGGACTGTCAAGTGGTCCCTGATAGTGAAAAAACTCCAAACGCCAATGATCACTTGTACATGAAGGCACTCTATCATGTTCTTCCAATGCAGTATGTGTCAATTGCGAAGCTTCAGAACAAGCTTGGAGGAGAAGCAAATCAGAATACTGTGCGCAAGATGATTGATAAGATGGTGCAAGAAGGTTTGGTTGAAGCCAAGGGAAATCGAAGGCTAGGAAAGCGTGTGCTCCATTCTGATATTACCAAGAAAAAGCTTATTGAAGTCCAGAAAGCTTTGAATTATGATGCAATGGATGTGGACAACATTGAGCCCCAAAGCAAGTCGAATCATCAAGATTTTTATCCAATTGCTAGTAACTACAGAGACACATCCACATGCGGTGCGCTCCACTCCATTGGATCAGATCTGACACGCATGAGAATAAGGGCTAATTCAAATGAACAGAGTCCAATGAGGAGTGAGCAGACTACTTCAATAAGAAAGGAGCATGGAAACACCCCCACAAGCCGGGCTCAGCCAGAAATTTCAAGAGAGAGTTTTGCACCTGGTAATGATGAAAACAGAGCAAATTGCAACACAAACTACTGTGATGAAGATGAGAGAGTGATGTGCAGTAGTAGGTCGACGCAAGACAAGCGTTCCAGGAAAACAAGCATGGTCAAGGAGCCTATTCTTCAGTACACAAAGCGCCAGAAATCTCAAGCCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

1164

Amino Acids

132.07

Weight (kDa)

5.21

Isoelectric Point (pI)

45.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 317 - 476 4.8e-59 NLI interacting factor-like phosphatase
HORMA PF02301 556 - 762 3.1e-62 HORMA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000056)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74620
fragaria_vesca FvH4_1g17310 FvH4_1g21860 FvH4_2g00301 FvH4_2g00500 FvH4_2g00510 FvH4_2g01040 FvH4_2g01040 FvH4_2g01040 FvH4_2g01511 FvH4_2g01521 FvH4_2g05410 FvH4_2g15860 FvH4_2g16170 FvH4_2g20442 FvH4_3g22600 FvH4_4g03111 FvH4_4g31810 FvH4_4g31840 FvH4_4g33571 FvH4_4g33580 FvH4_4g33600 FvH4_4g33620 FvH4_4g33620 FvH4_5g04820 FvH4_5g04820 FvH4_5g04821 FvH4_5g04821 FvH4_5g04830 FvH4_5g04833 FvH4_5g04833 FvH4_5g04834 FvH4_5g05562 FvH4_5g06150 FvH4_5g07970 FvH4_5g09910 FvH4_5g11280 FvH4_5g12010 FvH4_6g06910 FvH4_6g11580 FvH4_6g15110 FvH4_6g24322 FvH4_6g31360 FvH4_6g34080 FvH4_6g44500 FvH4_6g47060 FvH4_6g50581 FvH4_6g50641 FvH4_6g50650 FvH4_6g50650 FvH4_6g50981 FvH4_6g51090 FvH4_6g51180 FvH4_6g51730 FvH4_6g51740 FvH4_6g51870 FvH4_6g52331 FvH4_6g52340 FvH4_6g52350 FvH4_6g52530 FvH4_6g52530
malus_domestica MD14G1195700.v1.1
prunus_persica Prupe.6G084800_v2.0.a1 Prupe.7G084100_v2.0.a1 Prupe.8G048800_v2.0.a1
pyrus_communis pycom17g01390
rosa_chinensis RchiOBHm_Chr0c11g0499451 RchiOBHm_Chr1g0314791 RchiOBHm_Chr1g0340221 RchiOBHm_Chr1g0362121 RchiOBHm_Chr1g0362131 RchiOBHm_Chr2g0107491 RchiOBHm_Chr2g0127721 RchiOBHm_Chr2g0143901 RchiOBHm_Chr2g0161531 RchiOBHm_Chr2g0169511 RchiOBHm_Chr2g0171151 RchiOBHm_Chr2g0171221 RchiOBHm_Chr2g0171251 RchiOBHm_Chr2g0171261 RchiOBHm_Chr2g0171341 RchiOBHm_Chr2g0171561 RchiOBHm_Chr2g0171591 RchiOBHm_Chr2g0171601 RchiOBHm_Chr2g0172411 RchiOBHm_Chr2g0172441 RchiOBHm_Chr2g0172771 RchiOBHm_Chr2g0172881 RchiOBHm_Chr2g0173201 RchiOBHm_Chr2g0173211 RchiOBHm_Chr2g0173941 RchiOBHm_Chr2g0173951 RchiOBHm_Chr2g0175281 RchiOBHm_Chr3g0463081 RchiOBHm_Chr3g0482941 RchiOBHm_Chr3g0486241 RchiOBHm_Chr3g0488151 RchiOBHm_Chr3g0488191 RchiOBHm_Chr3g0488271 RchiOBHm_Chr3g0488351 RchiOBHm_Chr3g0488411 RchiOBHm_Chr3g0488491 RchiOBHm_Chr4g0410881 RchiOBHm_Chr4g0440431 RchiOBHm_Chr4g0440501 RchiOBHm_Chr6g0269041 RchiOBHm_Chr7g0194201 RchiOBHm_Chr7g0194301 RchiOBHm_Chr7g0194311 RchiOBHm_Chr7g0194391 RchiOBHm_Chr7g0194401 RchiOBHm_Chr7g0236181
rosa_laevigata RLG00000004203 RLG00000006176 RLG00000022170 RLG00000022198 RLG00000022237 RLG00000022911
rosa_multiflora Rmu_co8086672.1_g000001 Rmu_co8091660.1_g000001 Rmu_co8325445.1_g000001 Rmu_co8482323.1_g000001 Rmu_sc0000239.1_g000034 Rmu_sc0000467.1_g000001 Rmu_sc0000766.1_g000038 Rmu_sc0000789.1_g000004 Rmu_sc0000831.1_g000055 Rmu_sc0000831.1_g000056 Rmu_sc0000831.1_g000057 Rmu_sc0000831.1_g000058 Rmu_sc0000831.1_g000059 Rmu_sc0000831.1_g000060 Rmu_sc0000901.1_g000011 Rmu_sc0000901.1_g000029 Rmu_sc0001513.1_g000006 Rmu_sc0001513.1_g000011 Rmu_sc0001576.1_g000034 Rmu_sc0001685.1_g000062 Rmu_sc0002784.1_g000036 Rmu_sc0003188.1_g000026 Rmu_sc0003188.1_g000027 Rmu_sc0003314.1_g000017 Rmu_sc0003548.1_g000019 Rmu_sc0003624.1_g000018 Rmu_sc0005193.1_g000015 Rmu_sc0006328.1_g000001 Rmu_sc0006328.1_g000005 Rmu_sc0006806.1_g000014 Rmu_sc0006806.1_g000028 Rmu_sc0007320.1_g000005 Rmu_sc0007493.1_g000003 Rmu_sc0007646.1_g000011 Rmu_sc0007648.1_g000001 Rmu_sc0007661.1_g000007 Rmu_sc0007684.1_g000008 Rmu_sc0007799.1_g000007 Rmu_sc0007841.1_g000004 Rmu_sc0008051.1_g000003 Rmu_sc0008124.1_g000008 Rmu_sc0009104.1_g000008 Rmu_sc0009433.1_g000012 Rmu_sc0009883.1_g000011 Rmu_sc0010744.1_g000003 Rmu_sc0011052.1_g000003 Rmu_sc0012403.1_g000008 Rmu_sc0013831.1_g000001 Rmu_sc0016754.1_g000004 Rmu_sc0022238.1_g000002 Rmu_sc0023157.1_g000001 Rmu_sc0023894.1_g000001 Rmu_sc0028130.1_g000001 Rmu_sc0040543.1_g000001 Rmu_ssc0000252.1_g000003 Rmu_ssc0000252.1_g000004 Rmu_ssc0000261.1_g000030 Rmu_ssc0000326.1_g000007
rosa_roxburghii Rroxscaffold_157G00438560 Rroxscaffold_2G00078410 Rroxscaffold_2G00078970 Rroxscaffold_2G00079030 Rroxscaffold_2G00079120 Rroxscaffold_2G00079340 Rroxscaffold_2G00079720 Rroxscaffold_2G00079870 Rroxscaffold_2G00079910 Rroxscaffold_2G00080570 Rroxscaffold_2G00080720 Rroxscaffold_2G00080770 Rroxscaffold_2G00080780 Rroxscaffold_2G00080850 Rroxscaffold_2G00080910 Rroxscaffold_2G00116750 Rroxscaffold_3G00261370 Rroxscaffold_3G00261500 Rroxscaffold_3G00266200 Rroxscaffold_4G00305290 Rroxscaffold_4G00311110 Rroxscaffold_4G00327720 Rroxscaffold_5G00381290 Rroxscaffold_5G00381320 Rroxscaffold_6G00394220 Rroxscaffold_6G00416970
rosa_rugosa Rorug01G0164800.1 Rorug01G0164900 Rorug01G0293800 Rorug01G0293900 Rorug01G0294200 Rorug02G0144400 Rorug02G0271300 Rorug02G0271400 Rorug02G0271400 Rorug02G0271500 Rorug02G0384900 Rorug02G0384900 Rorug02G0385000 Rorug02G0385100 Rorug02G0553000 Rorug02G0553100 Rorug02G0555000 Rorug02G0560000 Rorug02G0560100 Rorug02G0561500 Rorug02G0566500 Rorug02G0568800 Rorug02G0569000 Rorug02G0569400 Rorug02G0569700 Rorug02G0575200 Rorug02G0582300 Rorug03G0061300 Rorug03G0126300 Rorug03G0202400 Rorug03G0224200 Rorug03G0238200 Rorug03G0238300 Rorug03G0238400 Rorug03G0255100 Rorug03G0255100 Rorug04G0326000 Rorug04G0326200 Rorug04G0326300 Rorug06G0124000 Rorug07G0015300 Rorug07G0015700 Rorug07G0015700 Rorug07G0265000
rosa_samantha Rh1AG304100 Rh1AG304500 Rh1AG304600 Rh1CG166600 Rh1CG285500 Rh1DG218600 Rh2AG323600 Rh2AG611800 Rh2AG626100 Rh2AG626400 Rh2AG627700 Rh2AG629700 Rh2AG634400 Rh2AG634500 Rh2AG634800 Rh2AG652800 Rh2BG206800 Rh2BG636300 Rh2BG636400 Rh2BG637300 Rh2BG637400 Rh2BG642600 Rh2BG648000 Rh2BG648300 Rh2BG652700 Rh2BG664000 Rh2CG311300 Rh2CG604800 Rh2CG605400 Rh2CG606300 Rh2CG606800 Rh2CG623800 Rh2CG628700 Rh2DG201600 Rh2DG351100 Rh2DG648000 Rh2DG648100 Rh2DG648900 Rh2DG649000 Rh2DG649100 Rh2DG651000 Rh2DG654900 Rh2DG655100 Rh2DG655200 Rh2DG656700 Rh2DG656900 Rh2DG657100 Rh2DG662700 Rh2DG662900 Rh2DG666300 Rh2DG667000 Rh2DG670700 Rh2DG672100 Rh2DG673400 Rh2DG673600 Rh2DG675200 Rh2DG675300 Rh2DG675600 Rh2DG679000 Rh2DG679100 Rh3AG048000 Rh3AG048100 Rh3AG106800 Rh3AG119500 Rh3AG251300 Rh3AG285800 Rh3AG286700 Rh3AG287300 Rh3AG287400 Rh3CG049400 Rh3CG321600 Rh3DG320300 Rh4AG377300 Rh4AG377700 Rh4AG377900 Rh4DG159200 Rh7AG064400 Rh7AG097600 Rh7AG141500 Rh7AG142100 Rh7AG142200 Rh7AG142300 Rh7AG142600 Rh7AG142800 Rh7AG417800 Rh7AG424000 Rh7CG147100 Rh7DG143000 Rh7DG143900 Rh7DG144400 Rh7DG144700
rosa_wichuraiana Rw0G019520 Rw1G014950 Rw1G027080 Rw2G015390 Rw2G035650 Rw2G046060 Rw2G050780 Rw2G050810 Rw2G051810 Rw2G051850 Rw2G051940 Rw2G051990 Rw2G052000 Rw2G052130 Rw2G052140 Rw2G052530 Rw2G052760 Rw2G052830 Rw2G053180 Rw2G053330 Rw2G053630 Rw3G010110 Rw3G024300 Rw3G025400 Rw3G025450 Rw4G032320 Rw4G032440 Rw6G037400 Rw7G012180 Rw7G012220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 622
Acc16I TGCGCA 1 cut(s) 2936
Acc36I ACCTGC 1 cut(s) 1695
AccI GTMKAC 2 cut(s) 1290, 3407
AccII CGCG 1 cut(s) 1545
AccIII TCCGGA 1 cut(s) 1735
AciI CCGC 5 cut(s) 1430, 1545, 1646, 2086, 3162
AcoI YGGCCR 3 cut(s) 607, 902, 1261
AcuI CTGAAG 8 cut(s) 738, 1658, 2148, 2185, 2685, 2712, 2879, 3443
AcvI CACGTG 1 cut(s) 1277
AcyI GRCGYC 2 cut(s) 1854, 2174
AfaI GTAC 7 cut(s) 331, 575, 806, 978, 1871, 2840, 3464
AfiI CCNNNNNNNGG 6 cut(s) 900, 1270, 1735, 2092, 2388, 3291
AflIII ACRYGT 2 cut(s) 683, 1276
AjnI CCWGG 3 cut(s) 1965, 3328, 3425
AjuI GAANNNNNNNTTGG 2 cut(s) 1392, 1424
AloI GAACNNNNNNTCC 2 cut(s) 127, 159
Alw21I GWGCWC 2 cut(s) 196, 3012
Alw26I GTCTC 7 cut(s) 268, 350, 608, 727, 1556, 1818, 3141
Aor13HI TCCGGA 1 cut(s) 1735
AoxI GGCC 6 cut(s) 18, 607, 902, 1261, 1726, 1913
ApeKI GCWGC 6 cut(s) 346, 431, 910, 913, 1153, 2595
Asp700I GAANNNNTTC 4 cut(s) 157, 1422, 1745, 2697
AspLEI GCGC 4 cut(s) 2136, 2937, 3169, 3474
AspS9I GGNCC 3 cut(s) 1913, 2083, 2797
AsuC2I CCSGG 3 cut(s) 364, 1457, 3293
AsuHPI GGTGA 3 cut(s) 570, 1514, 1645
AsuII TTCGAA 1 cut(s) 634
AvaII GGWCC 2 cut(s) 2083, 2797
AxyI CCTNAGG 1 cut(s) 2496
BaeI ACNNNNGTAYC 2 cut(s) 222, 255
BalI TGGCCA 1 cut(s) 609
BamHI GGATCC 2 cut(s) 1171, 2542
BanII GRGCYC 3 cut(s) 196, 3096, 3298
BbrPI CACGTG 1 cut(s) 1277
Bbv12I GWGCWC 2 cut(s) 196, 3012
BbvI GCAGC 6 cut(s) 333, 418, 897, 900, 1165, 2607
BccI CCATC 5 cut(s) 556, 1203, 1828, 2607, 2947
BceAI ACGGC 3 cut(s) 889, 1050, 1248
BcgI CGANNNNNNTGC 2 cut(s) 1669, 1703
BciT130I CCWGG 3 cut(s) 1967, 3330, 3427
BciVI GTATCC 1 cut(s) 223
BclI TGATCA 2 cut(s) 2752, 2830
BcnI CCSGG 3 cut(s) 364, 1457, 3293
BcoDI GTCTC 7 cut(s) 268, 350, 608, 727, 1556, 1818, 3141
BfaI CTAG 6 cut(s) 162, 989, 1131, 2571, 2996, 3135
BfmI CTRYAG 2 cut(s) 2526, 3142
BfoI RGCGCY 1 cut(s) 3475
BfuAI ACCTGC 1 cut(s) 1695
BfuI GTATCC 1 cut(s) 223
BglII AGATCT 1 cut(s) 3187
BisI GCNGC 6 cut(s) 347, 432, 911, 914, 1154, 2596
BlpI GCTNAGC 2 cut(s) 2463, 3297
BlsI GCNGC 6 cut(s) 348, 433, 912, 915, 1155, 2597
BmcAI AGTACT 1 cut(s) 575
Bme1390I CCNGG 6 cut(s) 364, 1457, 1967, 3293, 3330, 3427
Bme18I GGWCC 2 cut(s) 2083, 2797
BmgT120I GGNCC 3 cut(s) 1913, 2083, 2797
BmiI GGNNCC 8 cut(s) 306, 786, 1173, 1316, 1685, 2544, 2799, 3450
BmrFI CCNGG 6 cut(s) 364, 1457, 1967, 3293, 3330, 3427
BpmI CTGGAG 2 cut(s) 2279, 2733
Bpu10I CCTNAGC 2 cut(s) 918, 2368
Bpu1102I GCTNAGC 2 cut(s) 2463, 3297
Bpu14I TTCGAA 1 cut(s) 634
BpuEI CTTGAG 2 cut(s) 2285, 3468
BpuMI CCSGG 3 cut(s) 364, 1457, 3293
Bsa29I ATCGAT 1 cut(s) 2440
BsaAI YACGTR 2 cut(s) 808, 1277
BsaBI GATNNNNATC 3 cut(s) 1118, 2346, 3123
BsaHI GRCGYC 2 cut(s) 1854, 2174
BsaJI CCNNGG 5 cut(s) 180, 363, 595, 2234, 2979
BsaWI WCCGGW 1 cut(s) 1735
Bsc4I CCNNNNNNNGG 6 cut(s) 900, 1270, 1735, 2092, 2388, 3291
Bse1I ACTGG 8 cut(s) 67, 174, 1570, 2097, 2177, 2262, 2588, 2716
Bse21I CCTNAGG 1 cut(s) 2496
Bse3DI GCAATG 1 cut(s) 3078
Bse8I GATNNNNATC 3 cut(s) 1118, 2346, 3123
BseAI TCCGGA 1 cut(s) 1735
BseBI CCWGG 3 cut(s) 1967, 3330, 3427
BseCI ATCGAT 1 cut(s) 2440
BseDI CCNNGG 5 cut(s) 180, 363, 595, 2234, 2979
BseJI GATNNNNATC 3 cut(s) 1118, 2346, 3123
BseLI CCNNNNNNNGG 6 cut(s) 900, 1270, 1735, 2092, 2388, 3291
BseMI GCAATG 1 cut(s) 3078
BseMII CTCAG 9 cut(s) 87, 209, 738, 897, 1090, 2510, 2552, 2664, 3311
BseNI ACTGG 8 cut(s) 67, 174, 1570, 2097, 2177, 2262, 2588, 2716
BseRI GAGGAG 4 cut(s) 317, 437, 2925, 3253
BseX3I CGGCCG 1 cut(s) 902
BseXI GCAGC 6 cut(s) 333, 418, 897, 900, 1165, 2607
BsgI GTGCAG 1 cut(s) 3415
Bsh1236I CGCG 1 cut(s) 1545
Bsh1285I CGRYCG 2 cut(s) 905, 1430
BshFI GGCC 6 cut(s) 20, 609, 904, 1263, 1728, 1915
BshVI ATCGAT 1 cut(s) 2440
BsiEI CGRYCG 2 cut(s) 905, 1430
BsiHKAI GWGCWC 2 cut(s) 196, 3012
BsiSI CCGG 4 cut(s) 363, 1456, 1736, 3292
BslFI GGGAC 5 cut(s) 162, 298, 875, 2732, 2783
BslI CCNNNNNNNGG 6 cut(s) 900, 1270, 1735, 2092, 2388, 3291
BsmAI GTCTC 7 cut(s) 268, 350, 608, 727, 1556, 1818, 3141
BsmFI GGGAC 5 cut(s) 162, 298, 875, 2732, 2783
BsnI GGCC 6 cut(s) 20, 609, 904, 1263, 1728, 1915
Bsp119I TTCGAA 1 cut(s) 634
Bsp1286I GDGCHC 4 cut(s) 196, 3012, 3096, 3298
Bsp13I TCCGGA 1 cut(s) 1735
Bsp1407I TGTACA 1 cut(s) 2838
Bsp1720I GCTNAGC 2 cut(s) 2463, 3297
Bsp19I CCATGG 2 cut(s) 595, 2234
BspACI CCGC 5 cut(s) 1430, 1545, 1646, 2086, 3162
BspANI GGCC 6 cut(s) 20, 609, 904, 1263, 1728, 1915
BspCNI CTCAG 9 cut(s) 86, 208, 737, 896, 1089, 2509, 2551, 2663, 3310
BspDI ATCGAT 1 cut(s) 2440
BspEI TCCGGA 1 cut(s) 1735
BspFNI CGCG 1 cut(s) 1545
BspHI TCATGA 1 cut(s) 2755
BspLI GGNNCC 8 cut(s) 306, 786, 1173, 1316, 1685, 2544, 2799, 3450
BspMI ACCTGC 1 cut(s) 1695
BspQI GCTCTTC 1 cut(s) 2124
BspT104I TTCGAA 1 cut(s) 634
BsrDI GCAATG 1 cut(s) 3078
BsrGI TGTACA 1 cut(s) 2838
BsrI ACTGG 8 cut(s) 67, 174, 1570, 2097, 2177, 2262, 2588, 2716
BssECI CCNNGG 5 cut(s) 180, 363, 595, 2234, 2979
BssNI GRCGYC 2 cut(s) 1854, 2174
BssT1I CCWWGG 4 cut(s) 180, 595, 2234, 2979
Bst2UI CCWGG 3 cut(s) 1967, 3330, 3427
Bst4CI ACNGT 8 cut(s) 631, 653, 1051, 2414, 2723, 2789, 2932, 3371
Bst6I CTCTTC 4 cut(s) 1172, 1896, 2124, 2404
BstACI GRCGYC 2 cut(s) 1854, 2174
BstAUI TGTACA 1 cut(s) 2838
BstBAI YACGTR 2 cut(s) 808, 1277
BstBI TTCGAA 1 cut(s) 634
BstC8I GCNNGC 3 cut(s) 319, 2282, 2315
BstDSI CCRYGG 2 cut(s) 595, 2234
BstFNI CGCG 1 cut(s) 1545
BstH2I RGCGCY 1 cut(s) 3475
BstHHI GCGC 4 cut(s) 2136, 2937, 3169, 3474
BstMAI GTCTC 7 cut(s) 268, 350, 608, 727, 1556, 1818, 3141
BstMCI CGRYCG 2 cut(s) 905, 1430
BstMWI GCNNNNNNNGC 7 cut(s) 910, 919, 1666, 2131, 2459, 3001, 3418
BstNI CCWGG 3 cut(s) 1967, 3330, 3427
BstNSI RCATGY 3 cut(s) 488, 687, 3162
BstSCI CCNGG 6 cut(s) 362, 1455, 1965, 3291, 3328, 3425
BstSFI CTRYAG 2 cut(s) 2526, 3142
BstUI CGCG 1 cut(s) 1545
BstV1I GCAGC 6 cut(s) 333, 418, 897, 900, 1165, 2607
BstX2I RGATCY 4 cut(s) 65, 1171, 2542, 3187
BstXI CCANNNNNNTGG 1 cut(s) 3179
BstYI RGATCY 4 cut(s) 65, 1171, 2542, 3187
BstZI CGGCCG 1 cut(s) 902
Bsu15I ATCGAT 1 cut(s) 2440
Bsu36I CCTNAGG 1 cut(s) 2496
BsuI GTATCC 1 cut(s) 223
BsuRI GGCC 6 cut(s) 20, 609, 904, 1263, 1728, 1915
BsuTUI ATCGAT 1 cut(s) 2440
BtgI CCRYGG 2 cut(s) 595, 2234
BtgZI GCGATG 1 cut(s) 1681
BtsIMutI CAGTG 4 cut(s) 627, 2090, 2419, 2658
BveI ACCTGC 1 cut(s) 1695
Cac8I GCNNGC 3 cut(s) 319, 2282, 2315
CciI TCATGA 1 cut(s) 2755
CfoI GCGC 4 cut(s) 2136, 2937, 3169, 3474
Cfr13I GGNCC 3 cut(s) 1913, 2083, 2797
ClaI ATCGAT 1 cut(s) 2440
CseI GACGC 3 cut(s) 1862, 2182, 3418
CsiI ACCWGGT 1 cut(s) 3328
Csp6I GTAC 7 cut(s) 330, 574, 805, 977, 1870, 2839, 3463
CspCI CAANNNNNGTGG 2 cut(s) 3060, 3095
CviQI GTAC 7 cut(s) 330, 574, 805, 977, 1870, 2839, 3463
DraI TTTAAA 1 cut(s) 1617
DrdI GACNNNNNNGTC 1 cut(s) 622
DseDI GACNNNNNNGTC 1 cut(s) 622
EaeI YGGCCR 3 cut(s) 607, 902, 1261
EagI CGGCCG 1 cut(s) 902
Eam1104I CTCTTC 4 cut(s) 1172, 1896, 2124, 2404
EarI CTCTTC 4 cut(s) 1172, 1896, 2124, 2404
EciI GGCGGA 1 cut(s) 1661
Ecl136II GAGCTC 1 cut(s) 194
EclXI CGGCCG 1 cut(s) 902
Eco130I CCWWGG 4 cut(s) 180, 595, 2234, 2979
Eco147I AGGCCT 1 cut(s) 1728
Eco24I GRGCYC 3 cut(s) 196, 3096, 3298
Eco47I GGWCC 2 cut(s) 2083, 2797
Eco52I CGGCCG 1 cut(s) 902
Eco53kI GAGCTC 1 cut(s) 194
Eco57I CTGAAG 8 cut(s) 738, 1658, 2148, 2185, 2685, 2712, 2879, 3443
Eco72I CACGTG 1 cut(s) 1277
Eco81I CCTNAGG 1 cut(s) 2496
EcoICRI GAGCTC 1 cut(s) 194
EcoRII CCWGG 3 cut(s) 1965, 3328, 3425
EcoT14I CCWWGG 4 cut(s) 180, 595, 2234, 2979
EcoT38I GRGCYC 3 cut(s) 196, 3096, 3298
ErhI CCWWGG 4 cut(s) 180, 595, 2234, 2979
FalI AAGNNNNNCTT 4 cut(s) 142, 174, 2056, 2088
FaqI GGGAC 5 cut(s) 162, 298, 875, 2732, 2783
FauI CCCGC 1 cut(s) 1538
FbaI TGATCA 2 cut(s) 2752, 2830
FblI GTMKAC 2 cut(s) 1290, 3407
Fnu4HI GCNGC 6 cut(s) 347, 432, 911, 914, 1154, 2596
FriOI GRGCYC 3 cut(s) 196, 3096, 3298
Fsp4HI GCNGC 6 cut(s) 347, 432, 911, 914, 1154, 2596
FspBI CTAG 6 cut(s) 162, 989, 1131, 2571, 2996, 3135
FspI TGCGCA 1 cut(s) 2936
GlaI GCGC 4 cut(s) 2135, 2936, 3168, 3473
GluI GCNGC 6 cut(s) 347, 432, 911, 914, 1154, 2596
GsuI CTGGAG 2 cut(s) 2279, 2733
HaeII RGCGCY 1 cut(s) 3475
HaeIII GGCC 6 cut(s) 20, 609, 904, 1263, 1728, 1915
HapII CCGG 4 cut(s) 363, 1456, 1736, 3292
HgaI GACGC 3 cut(s) 1862, 2182, 3418
HhaI GCGC 4 cut(s) 2136, 2937, 3169, 3474
Hin1I GRCGYC 2 cut(s) 1854, 2174
Hin6I GCGC 4 cut(s) 2134, 2935, 3167, 3472
HinP1I GCGC 4 cut(s) 2134, 2935, 3167, 3472
HincII GTYRAC 5 cut(s) 795, 1291, 2422, 2506, 3408
HindII GTYRAC 5 cut(s) 795, 1291, 2422, 2506, 3408
HindIII AAGCTT 6 cut(s) 47, 1402, 2891, 2903, 3035, 3054
HpaI GTTAAC 2 cut(s) 2422, 2506
HpaII CCGG 4 cut(s) 363, 1456, 1736, 3292
HphI GGTGA 3 cut(s) 570, 1514, 1645
Hpy99I CGWCG 1 cut(s) 3412
HpyAV CCTTC 7 cut(s) 10, 1633, 1904, 2687, 2839, 2957, 2985
HpyCH4III ACNGT 8 cut(s) 631, 653, 1051, 2414, 2723, 2789, 2932, 3371
HpyCH4IV ACGT 2 cut(s) 807, 1276
HpyF10VI GCNNNNNNNGC 7 cut(s) 910, 919, 1666, 2131, 2459, 3001, 3418
HpySE526I ACGT 2 cut(s) 807, 1276
Hsp92I GRCGYC 2 cut(s) 1854, 2174
HspAI GCGC 4 cut(s) 2134, 2935, 3167, 3472
Kpn2I TCCGGA 1 cut(s) 1735
Ksp22I TGATCA 2 cut(s) 2752, 2830
KspAI GTTAAC 2 cut(s) 2422, 2506
LguI GCTCTTC 1 cut(s) 2124
LmnI GCTCC 6 cut(s) 304, 2372, 3015, 3174, 3268, 3448
Lsp1109I GCAGC 6 cut(s) 333, 418, 897, 900, 1165, 2607
MabI ACCWGGT 1 cut(s) 3328
MaeI CTAG 6 cut(s) 162, 989, 1131, 2571, 2996, 3135
MaeII ACGT 2 cut(s) 807, 1276
MfeI CAATTG 3 cut(s) 1518, 2883, 3129
MflI RGATCY 4 cut(s) 65, 1171, 2542, 3187
MhlI GDGCHC 4 cut(s) 196, 3012, 3096, 3298
MlsI TGGCCA 1 cut(s) 609
MluNI TGGCCA 1 cut(s) 609
MlyI GAGTC 9 cut(s) 270, 280, 632, 919, 1820, 2024, 2372, 2390, 3238
MmeI TCCRAC 3 cut(s) 60, 738, 1390
Mox20I TGGCCA 1 cut(s) 609
MroI TCCGGA 1 cut(s) 1735
MroXI GAANNNNTTC 4 cut(s) 157, 1422, 1745, 2697
MscI TGGCCA 1 cut(s) 609
MslI CAYNNNNRTG 7 cut(s) 213, 489, 600, 2023, 2144, 2651, 3157
Msp20I TGGCCA 1 cut(s) 609
MspI CCGG 4 cut(s) 363, 1456, 1736, 3292
MspR9I CCNGG 6 cut(s) 364, 1457, 1967, 3293, 3330, 3427
MunI CAATTG 3 cut(s) 1518, 2883, 3129
MvaI CCWGG 3 cut(s) 1967, 3330, 3427
MvnI CGCG 1 cut(s) 1545
MwoI GCNNNNNNNGC 7 cut(s) 910, 919, 1666, 2131, 2459, 3001, 3418
NciI CCSGG 3 cut(s) 364, 1457, 3293
NcoI CCATGG 2 cut(s) 595, 2234
NlaIV GGNNCC 8 cut(s) 306, 786, 1173, 1316, 1685, 2544, 2799, 3450
NmuCI GTSAC 2 cut(s) 2027, 2170
NsbI TGCGCA 1 cut(s) 2936
NspI RCATGY 3 cut(s) 488, 687, 3162
NspV TTCGAA 1 cut(s) 634
PagI TCATGA 1 cut(s) 2755
PceI AGGCCT 1 cut(s) 1728
PciI ACATGT 1 cut(s) 683
PciSI GCTCTTC 1 cut(s) 2124
PcsI WCGNNNNNNNCGW 1 cut(s) 1204
PdmI GAANNNNTTC 4 cut(s) 157, 1422, 1745, 2697
PfeI GAWTC 6 cut(s) 497, 1211, 1505, 1664, 2245, 3109
PfoI TCCNGGA 2 cut(s) 1455, 3425
PkrI GCNGC 6 cut(s) 348, 433, 912, 915, 1155, 2597
PleI GAGTC 9 cut(s) 270, 280, 631, 919, 1819, 2024, 2372, 2390, 3237
PmaCI CACGTG 1 cut(s) 1277
PmlI CACGTG 1 cut(s) 1277
PpsI GAGTC 9 cut(s) 270, 280, 631, 919, 1819, 2024, 2372, 2390, 3237
Ppu21I YACGTR 2 cut(s) 808, 1277
PscI ACATGT 1 cut(s) 683
Psp124BI GAGCTC 1 cut(s) 196
Psp6I CCWGG 3 cut(s) 1965, 3328, 3425
PspCI CACGTG 1 cut(s) 1277
PspGI CCWGG 3 cut(s) 1965, 3328, 3425
PspN4I GGNNCC 8 cut(s) 306, 786, 1173, 1316, 1685, 2544, 2799, 3450
PspPI GGNCC 3 cut(s) 1913, 2083, 2797
PsrI GAACNNNNNNTAC 2 cut(s) 1407, 1439
PsuI RGATCY 4 cut(s) 65, 1171, 2542, 3187
RsaI GTAC 7 cut(s) 331, 575, 806, 978, 1871, 2840, 3464
RsaNI GTAC 7 cut(s) 330, 574, 805, 977, 1870, 2839, 3463
RseI CAYNNNNRTG 7 cut(s) 213, 489, 600, 2023, 2144, 2651, 3157
SacI GAGCTC 1 cut(s) 196
SalI GTCGAC 2 cut(s) 1289, 3406
SapI GCTCTTC 1 cut(s) 2124
SatI GCNGC 6 cut(s) 347, 432, 911, 914, 1154, 2596
Sau96I GGNCC 3 cut(s) 1913, 2083, 2797
ScaI AGTACT 1 cut(s) 575
SchI GAGTC 9 cut(s) 270, 280, 632, 919, 1820, 2024, 2372, 2390, 3238
ScrFI CCNGG 6 cut(s) 364, 1457, 1967, 3293, 3330, 3427
SduI GDGCHC 4 cut(s) 196, 3012, 3096, 3298
SexAI ACCWGGT 1 cut(s) 3328
SfcI CTRYAG 2 cut(s) 2526, 3142
SfuI TTCGAA 1 cut(s) 634
SinI GGWCC 2 cut(s) 2083, 2797
SmiMI CAYNNNNRTG 7 cut(s) 213, 489, 600, 2023, 2144, 2651, 3157
SmlI CTYRAG 2 cut(s) 2300, 3483
SmoI CTYRAG 2 cut(s) 2300, 3483
SseBI AGGCCT 1 cut(s) 1728
SsiI CCGC 5 cut(s) 1430, 1545, 1646, 2086, 3162
SspI AATATT 1 cut(s) 709
SspMI CTAG 6 cut(s) 162, 989, 1131, 2571, 2996, 3135
SstI GAGCTC 1 cut(s) 196
StuI AGGCCT 1 cut(s) 1728
StyD4I CCNGG 6 cut(s) 362, 1455, 1965, 3291, 3328, 3425
StyI CCWWGG 4 cut(s) 180, 595, 2234, 2979
TaaI ACNGT 8 cut(s) 631, 653, 1051, 2414, 2723, 2789, 2932, 3371
TaiI ACGT 2 cut(s) 810, 1279
TatI WGTACW 4 cut(s) 329, 573, 2838, 3462
TfiI GAWTC 6 cut(s) 497, 1211, 1505, 1664, 2245, 3109
TscAI CASTG 4 cut(s) 634, 2097, 2419, 2658
TseFI GTSAC 2 cut(s) 2027, 2170
TseI GCWGC 6 cut(s) 346, 431, 910, 913, 1153, 2595
Tsp45I GTSAC 2 cut(s) 2027, 2170
TspRI CASTG 4 cut(s) 634, 2097, 2419, 2658
VpaK11BI GGWCC 2 cut(s) 2083, 2797
XbaI TCTAGA 1 cut(s) 161
XceI RCATGY 3 cut(s) 488, 687, 3162
XcmI CCANNNNNNNNNTGG 2 cut(s) 602, 1325
XmiI GTMKAC 2 cut(s) 1290, 3407
XmnI GAANNNNTTC 4 cut(s) 157, 1422, 1745, 2697
XspI CTAG 6 cut(s) 162, 989, 1131, 2571, 2996, 3135
ZrmI AGTACT 1 cut(s) 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.