FvH4_5g05562

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000056
Physical Location & Seq
Forward (+)
3273687 .. 3274223
537 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g05562.t1

Sequence Viewer

Length: 537 bp
ATGATGTCAAAAGAGTTGGGTTATATCCGATTCCACATGTGGAATGGCATGCGCTATACAAACCGATTAGACTTTACGTTTGTTCACTCTGTTGTGAAGCAACCGTCGCACGGGATGCTTGAAAGTGAGCTGCGTACGATTCTTTGGAGGTTTGGTGTCCCAGAAGCTAACCAGCCGCGTGTTATAGACGAAATATTGCAGGCGGTTCAAGTATCCCTTCCCATGCTGCCTATTGATGTAAATATATACCTAGAGCCGGTTCTAAGACTTCCACCGGCGGCTAGGCTGGAGCCGAACGTCAAATCGGCGACGGAATCGTCGATTGAGAGCCTGGAGAAAGTGAGGCTTGATAGTTTGGAAGAGTCACCTGAGTCATTGTGTACTATTTGTTCCGACGGCCTCGCAGATCAACTCATTGCTCGCATGCCTTGCTCACACTGTTTTCATGAACATTGCATCGTCCGGTGGCTGAAGATAAGTCACTTATGTCCCTTGTGCCGATATCCCATGCCAATCAGTAAGGAGGGCTTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

179

Amino Acids

20.41

Weight (kDa)

6.16

Isoelectric Point (pI)

59.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 124 - 167 7.3e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 126 - 167 3e-11 Ring finger domain
zf-C3HC4_2 PF13923 127 - 166 2.4e-07 Zinc finger, C3HC4 type (RING finger)
zf-ANAPC11 PF12861 142 - 170 2.1e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000056)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74620
fragaria_vesca FvH4_1g17310 FvH4_1g21860 FvH4_2g00301 FvH4_2g00500 FvH4_2g00510 FvH4_2g01040 FvH4_2g01040 FvH4_2g01040 FvH4_2g01511 FvH4_2g01521 FvH4_2g05410 FvH4_2g15860 FvH4_2g16170 FvH4_2g20442 FvH4_3g22600 FvH4_4g03111 FvH4_4g31810 FvH4_4g31840 FvH4_4g33571 FvH4_4g33580 FvH4_4g33600 FvH4_4g33620 FvH4_4g33620 FvH4_5g04820 FvH4_5g04820 FvH4_5g04821 FvH4_5g04821 FvH4_5g04830 FvH4_5g04833 FvH4_5g04833 FvH4_5g04834 FvH4_5g05562 FvH4_5g06150 FvH4_5g07970 FvH4_5g09910 FvH4_5g11280 FvH4_5g12010 FvH4_6g06910 FvH4_6g11580 FvH4_6g15110 FvH4_6g24322 FvH4_6g31360 FvH4_6g34080 FvH4_6g44500 FvH4_6g47060 FvH4_6g50581 FvH4_6g50641 FvH4_6g50650 FvH4_6g50650 FvH4_6g50981 FvH4_6g51090 FvH4_6g51180 FvH4_6g51730 FvH4_6g51740 FvH4_6g51870 FvH4_6g52331 FvH4_6g52340 FvH4_6g52350 FvH4_6g52530 FvH4_6g52530
malus_domestica MD14G1195700.v1.1
prunus_persica Prupe.6G084800_v2.0.a1 Prupe.7G084100_v2.0.a1 Prupe.8G048800_v2.0.a1
pyrus_communis pycom17g01390
rosa_chinensis RchiOBHm_Chr0c11g0499451 RchiOBHm_Chr1g0314791 RchiOBHm_Chr1g0340221 RchiOBHm_Chr1g0362121 RchiOBHm_Chr1g0362131 RchiOBHm_Chr2g0107491 RchiOBHm_Chr2g0127721 RchiOBHm_Chr2g0143901 RchiOBHm_Chr2g0161531 RchiOBHm_Chr2g0169511 RchiOBHm_Chr2g0171151 RchiOBHm_Chr2g0171221 RchiOBHm_Chr2g0171251 RchiOBHm_Chr2g0171261 RchiOBHm_Chr2g0171341 RchiOBHm_Chr2g0171561 RchiOBHm_Chr2g0171591 RchiOBHm_Chr2g0171601 RchiOBHm_Chr2g0172411 RchiOBHm_Chr2g0172441 RchiOBHm_Chr2g0172771 RchiOBHm_Chr2g0172881 RchiOBHm_Chr2g0173201 RchiOBHm_Chr2g0173211 RchiOBHm_Chr2g0173941 RchiOBHm_Chr2g0173951 RchiOBHm_Chr2g0175281 RchiOBHm_Chr3g0463081 RchiOBHm_Chr3g0482941 RchiOBHm_Chr3g0486241 RchiOBHm_Chr3g0488151 RchiOBHm_Chr3g0488191 RchiOBHm_Chr3g0488271 RchiOBHm_Chr3g0488351 RchiOBHm_Chr3g0488411 RchiOBHm_Chr3g0488491 RchiOBHm_Chr4g0410881 RchiOBHm_Chr4g0440431 RchiOBHm_Chr4g0440501 RchiOBHm_Chr6g0269041 RchiOBHm_Chr7g0194201 RchiOBHm_Chr7g0194301 RchiOBHm_Chr7g0194311 RchiOBHm_Chr7g0194391 RchiOBHm_Chr7g0194401 RchiOBHm_Chr7g0236181
rosa_laevigata RLG00000004203 RLG00000006176 RLG00000022170 RLG00000022198 RLG00000022237 RLG00000022911
rosa_multiflora Rmu_co8086672.1_g000001 Rmu_co8091660.1_g000001 Rmu_co8325445.1_g000001 Rmu_co8482323.1_g000001 Rmu_sc0000239.1_g000034 Rmu_sc0000467.1_g000001 Rmu_sc0000766.1_g000038 Rmu_sc0000789.1_g000004 Rmu_sc0000831.1_g000055 Rmu_sc0000831.1_g000056 Rmu_sc0000831.1_g000057 Rmu_sc0000831.1_g000058 Rmu_sc0000831.1_g000059 Rmu_sc0000831.1_g000060 Rmu_sc0000901.1_g000011 Rmu_sc0000901.1_g000029 Rmu_sc0001513.1_g000006 Rmu_sc0001513.1_g000011 Rmu_sc0001576.1_g000034 Rmu_sc0001685.1_g000062 Rmu_sc0002784.1_g000036 Rmu_sc0003188.1_g000026 Rmu_sc0003188.1_g000027 Rmu_sc0003314.1_g000017 Rmu_sc0003548.1_g000019 Rmu_sc0003624.1_g000018 Rmu_sc0005193.1_g000015 Rmu_sc0006328.1_g000001 Rmu_sc0006328.1_g000005 Rmu_sc0006806.1_g000014 Rmu_sc0006806.1_g000028 Rmu_sc0007320.1_g000005 Rmu_sc0007493.1_g000003 Rmu_sc0007646.1_g000011 Rmu_sc0007648.1_g000001 Rmu_sc0007661.1_g000007 Rmu_sc0007684.1_g000008 Rmu_sc0007799.1_g000007 Rmu_sc0007841.1_g000004 Rmu_sc0008051.1_g000003 Rmu_sc0008124.1_g000008 Rmu_sc0009104.1_g000008 Rmu_sc0009433.1_g000012 Rmu_sc0009883.1_g000011 Rmu_sc0010744.1_g000003 Rmu_sc0011052.1_g000003 Rmu_sc0012403.1_g000008 Rmu_sc0013831.1_g000001 Rmu_sc0016754.1_g000004 Rmu_sc0022238.1_g000002 Rmu_sc0023157.1_g000001 Rmu_sc0023894.1_g000001 Rmu_sc0028130.1_g000001 Rmu_sc0040543.1_g000001 Rmu_ssc0000252.1_g000003 Rmu_ssc0000252.1_g000004 Rmu_ssc0000261.1_g000030 Rmu_ssc0000326.1_g000007
rosa_roxburghii Rroxscaffold_157G00438560 Rroxscaffold_2G00078410 Rroxscaffold_2G00078970 Rroxscaffold_2G00079030 Rroxscaffold_2G00079120 Rroxscaffold_2G00079340 Rroxscaffold_2G00079720 Rroxscaffold_2G00079870 Rroxscaffold_2G00079910 Rroxscaffold_2G00080570 Rroxscaffold_2G00080720 Rroxscaffold_2G00080770 Rroxscaffold_2G00080780 Rroxscaffold_2G00080850 Rroxscaffold_2G00080910 Rroxscaffold_2G00116750 Rroxscaffold_3G00261370 Rroxscaffold_3G00261500 Rroxscaffold_3G00266200 Rroxscaffold_4G00305290 Rroxscaffold_4G00311110 Rroxscaffold_4G00327720 Rroxscaffold_5G00381290 Rroxscaffold_5G00381320 Rroxscaffold_6G00394220 Rroxscaffold_6G00416970
rosa_rugosa Rorug01G0164800.1 Rorug01G0164900 Rorug01G0293800 Rorug01G0293900 Rorug01G0294200 Rorug02G0144400 Rorug02G0271300 Rorug02G0271400 Rorug02G0271400 Rorug02G0271500 Rorug02G0384900 Rorug02G0384900 Rorug02G0385000 Rorug02G0385100 Rorug02G0553000 Rorug02G0553100 Rorug02G0555000 Rorug02G0560000 Rorug02G0560100 Rorug02G0561500 Rorug02G0566500 Rorug02G0568800 Rorug02G0569000 Rorug02G0569400 Rorug02G0569700 Rorug02G0575200 Rorug02G0582300 Rorug03G0061300 Rorug03G0126300 Rorug03G0202400 Rorug03G0224200 Rorug03G0238200 Rorug03G0238300 Rorug03G0238400 Rorug03G0255100 Rorug03G0255100 Rorug04G0326000 Rorug04G0326200 Rorug04G0326300 Rorug06G0124000 Rorug07G0015300 Rorug07G0015700 Rorug07G0015700 Rorug07G0265000
rosa_samantha Rh1AG304100 Rh1AG304500 Rh1AG304600 Rh1CG166600 Rh1CG285500 Rh1DG218600 Rh2AG323600 Rh2AG611800 Rh2AG626100 Rh2AG626400 Rh2AG627700 Rh2AG629700 Rh2AG634400 Rh2AG634500 Rh2AG634800 Rh2AG652800 Rh2BG206800 Rh2BG636300 Rh2BG636400 Rh2BG637300 Rh2BG637400 Rh2BG642600 Rh2BG648000 Rh2BG648300 Rh2BG652700 Rh2BG664000 Rh2CG311300 Rh2CG604800 Rh2CG605400 Rh2CG606300 Rh2CG606800 Rh2CG623800 Rh2CG628700 Rh2DG201600 Rh2DG351100 Rh2DG648000 Rh2DG648100 Rh2DG648900 Rh2DG649000 Rh2DG649100 Rh2DG651000 Rh2DG654900 Rh2DG655100 Rh2DG655200 Rh2DG656700 Rh2DG656900 Rh2DG657100 Rh2DG662700 Rh2DG662900 Rh2DG666300 Rh2DG667000 Rh2DG670700 Rh2DG672100 Rh2DG673400 Rh2DG673600 Rh2DG675200 Rh2DG675300 Rh2DG675600 Rh2DG679000 Rh2DG679100 Rh3AG048000 Rh3AG048100 Rh3AG106800 Rh3AG119500 Rh3AG251300 Rh3AG285800 Rh3AG286700 Rh3AG287300 Rh3AG287400 Rh3CG049400 Rh3CG321600 Rh3DG320300 Rh4AG377300 Rh4AG377700 Rh4AG377900 Rh4DG159200 Rh7AG064400 Rh7AG097600 Rh7AG141500 Rh7AG142100 Rh7AG142200 Rh7AG142300 Rh7AG142600 Rh7AG142800 Rh7AG417800 Rh7AG424000 Rh7CG147100 Rh7DG143000 Rh7DG143900 Rh7DG144400 Rh7DG144700
rosa_wichuraiana Rw0G019520 Rw1G014950 Rw1G027080 Rw2G015390 Rw2G035650 Rw2G046060 Rw2G050780 Rw2G050810 Rw2G051810 Rw2G051850 Rw2G051940 Rw2G051990 Rw2G052000 Rw2G052130 Rw2G052140 Rw2G052530 Rw2G052760 Rw2G052830 Rw2G053180 Rw2G053330 Rw2G053630 Rw3G010110 Rw3G024300 Rw3G025400 Rw3G025450 Rw4G032320 Rw4G032440 Rw6G037400 Rw7G012180 Rw7G012220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 316
AccII CGCG 1 cut(s) 178
AciI CCGC 3 cut(s) 176, 203, 278
AcuI CTGAAG 1 cut(s) 491
AfaI GTAC 2 cut(s) 136, 382
AfiI CCNNNNNNNGG 2 cut(s) 110, 256
AflIII ACRYGT 1 cut(s) 36
AgsI TTSAA 2 cut(s) 122, 209
AjnI CCWGG 1 cut(s) 330
AluBI AGCT 2 cut(s) 130, 167
AluI AGCT 2 cut(s) 130, 167
AoxI GGCC 1 cut(s) 397
ApeKI GCWGC 2 cut(s) 130, 226
ArsI GACNNNNNNTTYG 2 cut(s) 62, 94
AspLEI GCGC 1 cut(s) 54
AsuHPI GGTGA 1 cut(s) 357
BbvI GCAGC 2 cut(s) 117, 213
BceAI ACGGC 1 cut(s) 412
BciT130I CCWGG 1 cut(s) 332
BciVI GTATCC 1 cut(s) 223
BfaI CTAG 2 cut(s) 251, 282
BfuI GTATCC 1 cut(s) 223
BisI GCNGC 4 cut(s) 131, 176, 227, 279
BlsI GCNGC 4 cut(s) 132, 177, 228, 280
Bme1390I CCNGG 1 cut(s) 332
BmiI GGNNCC 1 cut(s) 291
BmrFI CCNGG 1 cut(s) 332
BmsI GCATC 2 cut(s) 105, 465
BpmI CTGGAG 2 cut(s) 308, 353
BsaWI WCCGGW 1 cut(s) 462
BsaXI ACNNNNNCTCC 2 cut(s) 139, 169
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 256
Bse118I RCCGGY 2 cut(s) 256, 274
Bse3DI GCAATG 2 cut(s) 414, 451
BseBI CCWGG 1 cut(s) 332
BseGI GGATG 1 cut(s) 120
BseLI CCNNNNNNNGG 2 cut(s) 110, 256
BseMI GCAATG 2 cut(s) 414, 451
BseMII CTCAG 1 cut(s) 360
BseXI GCAGC 2 cut(s) 117, 213
Bsh1236I CGCG 1 cut(s) 178
BshFI GGCC 1 cut(s) 399
BsiSI CCGG 3 cut(s) 257, 275, 463
BsiWI CGTACG 1 cut(s) 134
BslFI GGGAC 2 cut(s) 143, 474
BslI CCNNNNNNNGG 2 cut(s) 110, 256
BsmFI GGGAC 2 cut(s) 143, 474
BsnI GGCC 1 cut(s) 399
Bsp143I GATC 1 cut(s) 406
BspACI CCGC 3 cut(s) 176, 203, 278
BspANI GGCC 1 cut(s) 399
BspCNI CTCAG 1 cut(s) 361
BspFNI CGCG 1 cut(s) 178
BspHI TCATGA 1 cut(s) 445
BspLI GGNNCC 1 cut(s) 291
BsrDI GCAATG 2 cut(s) 414, 451
BsrFI RCCGGY 2 cut(s) 256, 274
BssAI RCCGGY 2 cut(s) 256, 274
BssMI GATC 1 cut(s) 406
Bst2UI CCWGG 1 cut(s) 332
Bst4CI ACNGT 2 cut(s) 105, 440
Bst6I CTCTTC 1 cut(s) 354
BstAPI GCANNNNNTGC 2 cut(s) 115, 429
BstC8I GCNNGC 4 cut(s) 50, 201, 421, 425
BstDEI CTNAG 2 cut(s) 263, 369
BstF5I GGATG 1 cut(s) 120
BstFNI CGCG 1 cut(s) 178
BstHHI GCGC 1 cut(s) 54
BstKTI GATC 1 cut(s) 409
BstMBI GATC 1 cut(s) 406
BstMWI GCNNNNNNNGC 3 cut(s) 106, 115, 429
BstNI CCWGG 1 cut(s) 332
BstNSI RCATGY 3 cut(s) 40, 52, 427
BstSCI CCNGG 1 cut(s) 330
BstUI CGCG 1 cut(s) 178
BstV1I GCAGC 2 cut(s) 117, 213
BsuI GTATCC 1 cut(s) 223
BsuRI GGCC 1 cut(s) 399
BtsCI GGATG 1 cut(s) 120
BtsIMutI CAGTG 1 cut(s) 436
Cac8I GCNNGC 4 cut(s) 50, 201, 421, 425
CciI TCATGA 1 cut(s) 445
CfoI GCGC 1 cut(s) 54
Cfr10I RCCGGY 2 cut(s) 256, 274
Csp6I GTAC 2 cut(s) 135, 381
CviAII CATG 6 cut(s) 37, 49, 223, 424, 446, 508
CviQI GTAC 2 cut(s) 135, 381
DdeI CTNAG 2 cut(s) 263, 369
DpnI GATC 1 cut(s) 408
DpnII GATC 1 cut(s) 406
DrdI GACNNNNNNGTC 1 cut(s) 316
DseDI GACNNNNNNGTC 1 cut(s) 316
Eam1104I CTCTTC 1 cut(s) 354
EarI CTCTTC 1 cut(s) 354
Eco32I GATATC 1 cut(s) 503
Eco57I CTGAAG 1 cut(s) 491
EcoRII CCWGG 1 cut(s) 330
EcoRV GATATC 1 cut(s) 503
FaeI CATG 6 cut(s) 40, 52, 226, 427, 449, 511
FalI AAGNNNNNCTT 5 cut(s) 201, 233, 330, 362, 512
FaqI GGGAC 2 cut(s) 143, 474
FatI CATG 6 cut(s) 36, 48, 222, 423, 445, 507
Fnu4HI GCNGC 4 cut(s) 131, 176, 227, 279
FokI GGATG 1 cut(s) 127
Fsp4HI GCNGC 4 cut(s) 131, 176, 227, 279
FspBI CTAG 2 cut(s) 251, 282
GlaI GCGC 1 cut(s) 53
GluI GCNGC 4 cut(s) 131, 176, 227, 279
GsuI CTGGAG 2 cut(s) 308, 353
HaeIII GGCC 1 cut(s) 399
HapII CCGG 3 cut(s) 257, 275, 463
HhaI GCGC 1 cut(s) 54
Hin1II CATG 6 cut(s) 40, 52, 226, 427, 449, 511
Hin6I GCGC 1 cut(s) 52
HinP1I GCGC 1 cut(s) 52
HinfI GANTC 5 cut(s) 30, 139, 314, 362, 371
HpaII CCGG 3 cut(s) 257, 275, 463
HphI GGTGA 1 cut(s) 357
Hpy166II GTNNAC 2 cut(s) 85, 381
Hpy188I TCNGA 2 cut(s) 29, 394
Hpy188III TCNNGA 1 cut(s) 446
Hpy8I GTNNAC 2 cut(s) 85, 381
Hpy99I CGWCG 4 cut(s) 109, 313, 322, 398
HpyAV CCTTC 1 cut(s) 227
HpyCH4III ACNGT 2 cut(s) 105, 440
HpyCH4IV ACGT 2 cut(s) 77, 297
HpyCH4V TGCA 2 cut(s) 199, 456
HpyF10VI GCNNNNNNNGC 3 cut(s) 106, 115, 429
HpyF3I CTNAG 2 cut(s) 263, 369
HpySE526I ACGT 2 cut(s) 77, 297
Hsp92II CATG 6 cut(s) 40, 52, 226, 427, 449, 511
HspAI GCGC 1 cut(s) 52
Kzo9I GATC 1 cut(s) 406
LmnI GCTCC 1 cut(s) 289
Lsp1109I GCAGC 2 cut(s) 117, 213
LweI GCATC 2 cut(s) 105, 465
MaeI CTAG 2 cut(s) 251, 282
MaeII ACGT 2 cut(s) 77, 297
MaeIII GTNAC 2 cut(s) 363, 479
MalI GATC 1 cut(s) 408
MboI GATC 1 cut(s) 406
MboII GAAGA 2 cut(s) 371, 484
MlyI GAGTC 2 cut(s) 371, 380
MmeI TCCRAC 1 cut(s) 417
MnlI CCTC 4 cut(s) 141, 336, 410, 517
MseI TTAA 1 cut(s) 535
MspI CCGG 3 cut(s) 257, 275, 463
MspR9I CCNGG 1 cut(s) 332
MvaI CCWGG 1 cut(s) 332
MvnI CGCG 1 cut(s) 178
MwoI GCNNNNNNNGC 3 cut(s) 106, 115, 429
NdeII GATC 1 cut(s) 406
NlaIII CATG 6 cut(s) 40, 52, 226, 427, 449, 511
NlaIV GGNNCC 1 cut(s) 291
NmuCI GTSAC 2 cut(s) 363, 479
NspI RCATGY 3 cut(s) 40, 52, 427
PaeI GCATGC 2 cut(s) 52, 427
PagI TCATGA 1 cut(s) 445
PciI ACATGT 1 cut(s) 36
PcsI WCGNNNNNNNCGW 1 cut(s) 317
PfeI GAWTC 3 cut(s) 30, 139, 314
Pfl23II CGTACG 1 cut(s) 134
PkrI GCNGC 4 cut(s) 132, 177, 228, 280
PleI GAGTC 2 cut(s) 370, 379
PpsI GAGTC 2 cut(s) 370, 379
PscI ACATGT 1 cut(s) 36
Psp6I CCWGG 1 cut(s) 330
PspGI CCWGG 1 cut(s) 330
PspLI CGTACG 1 cut(s) 134
PspN4I GGNNCC 1 cut(s) 291
PsrI GAACNNNNNNTAC 2 cut(s) 373, 405
RsaI GTAC 2 cut(s) 136, 382
RsaNI GTAC 2 cut(s) 135, 381
SaqAI TTAA 1 cut(s) 535
SatI GCNGC 4 cut(s) 131, 176, 227, 279
Sau3AI GATC 1 cut(s) 406
SchI GAGTC 2 cut(s) 371, 380
ScrFI CCNGG 1 cut(s) 332
SetI ASST 7 cut(s) 80, 132, 152, 169, 252, 300, 370
SfaNI GCATC 2 cut(s) 105, 465
SgrAI CRCCGGYG 1 cut(s) 274
SphI GCATGC 2 cut(s) 52, 427
SsiI CCGC 3 cut(s) 176, 203, 278
SspI AATATT 1 cut(s) 195
SspMI CTAG 2 cut(s) 251, 282
StyD4I CCNGG 1 cut(s) 330
TaaI ACNGT 2 cut(s) 105, 440
TaiI ACGT 2 cut(s) 80, 300
TaqI TCGA 1 cut(s) 320
TatI WGTACW 1 cut(s) 380
TauI GCSGC 2 cut(s) 178, 281
TfiI GAWTC 3 cut(s) 30, 139, 314
Tru1I TTAA 1 cut(s) 535
Tru9I TTAA 1 cut(s) 535
TscAI CASTG 1 cut(s) 443
TseFI GTSAC 2 cut(s) 363, 479
TseI GCWGC 2 cut(s) 130, 226
Tsp45I GTSAC 2 cut(s) 363, 479
TspDTI ATGAA 2 cut(s) 434, 462
TspGWI ACGGA 1 cut(s) 326
TspRI CASTG 1 cut(s) 443
XceI RCATGY 3 cut(s) 40, 52, 427
XcmI CCANNNNNNNNNTGG 1 cut(s) 41
XspI CTAG 2 cut(s) 251, 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.