ubiquitin-protein transferase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 733 |
| Acc36I | ACCTGC | 1 cut(s) | 733 |
| AccB1I | GGYRCC | 1 cut(s) | 253 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 213 |
| AccI | GTMKAC | 1 cut(s) | 360 |
| AciI | CCGC | 1 cut(s) | 106 |
| AclWI | GGATC | 2 cut(s) | 116, 501 |
| AcsI | RAATTY | 1 cut(s) | 339 |
| AcuI | CTGAAG | 1 cut(s) | 701 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 12, 13, 213 |
| AflIII | ACRYGT | 1 cut(s) | 578 |
| AgsI | TTSAA | 2 cut(s) | 518, 526 |
| AhlI | ACTAGT | 1 cut(s) | 629 |
| AjnI | CCWGG | 1 cut(s) | 61 |
| AluBI | AGCT | 2 cut(s) | 79, 307 |
| AluI | AGCT | 2 cut(s) | 79, 307 |
| Alw26I | GTCTC | 1 cut(s) | 563 |
| AlwI | GGATC | 2 cut(s) | 116, 501 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 679 |
| Ama87I | CYCGRG | 1 cut(s) | 302 |
| ApoI | RAATTY | 1 cut(s) | 339 |
| AspLEI | GCGC | 2 cut(s) | 213, 644 |
| AsuHPI | GGTGA | 1 cut(s) | 638 |
| AvaI | CYCGRG | 1 cut(s) | 302 |
| BanI | GGYRCC | 1 cut(s) | 253 |
| BbsI | GAAGAC | 1 cut(s) | 227 |
| BccI | CCATC | 1 cut(s) | 787 |
| BciT130I | CCWGG | 1 cut(s) | 63 |
| BcoDI | GTCTC | 1 cut(s) | 563 |
| BcuI | ACTAGT | 1 cut(s) | 629 |
| BfaI | CTAG | 2 cut(s) | 504, 630 |
| BfmI | CTRYAG | 1 cut(s) | 94 |
| BfuAI | ACCTGC | 1 cut(s) | 733 |
| BisI | GCNGC | 1 cut(s) | 106 |
| BlsI | GCNGC | 1 cut(s) | 107 |
| Bme1390I | CCNGG | 1 cut(s) | 63 |
| BmeT110I | CYCGRG | 1 cut(s) | 302 |
| BmiI | GGNNCC | 2 cut(s) | 29, 255 |
| BmrFI | CCNGG | 1 cut(s) | 63 |
| BmsI | GCATC | 2 cut(s) | 406, 509 |
| BpiI | GAAGAC | 1 cut(s) | 227 |
| BpuEI | CTTGAG | 1 cut(s) | 284 |
| BsaJI | CCNNGG | 1 cut(s) | 7 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 12, 13, 213 |
| Bse1I | ACTGG | 1 cut(s) | 673 |
| Bse3DI | GCAATG | 1 cut(s) | 53 |
| BseBI | CCWGG | 1 cut(s) | 63 |
| BseDI | CCNNGG | 1 cut(s) | 7 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 12, 13, 213 |
| BseMI | GCAATG | 1 cut(s) | 53 |
| BseNI | ACTGG | 1 cut(s) | 673 |
| BsgI | GTGCAG | 2 cut(s) | 314, 711 |
| BshNI | GGYRCC | 1 cut(s) | 253 |
| BsiHKCI | CYCGRG | 1 cut(s) | 302 |
| BslFI | GGGAC | 6 cut(s) | 284, 684, 761, 767, 773, 779 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 12, 13, 213 |
| BsmAI | GTCTC | 1 cut(s) | 563 |
| BsmFI | GGGAC | 6 cut(s) | 284, 684, 761, 767, 773, 779 |
| BsmI | GAATGC | 1 cut(s) | 723 |
| BsoBI | CYCGRG | 1 cut(s) | 302 |
| Bsp143I | GATC | 4 cut(s) | 121, 399, 506, 688 |
| BspACI | CCGC | 1 cut(s) | 106 |
| BspHI | TCATGA | 1 cut(s) | 139 |
| BspLI | GGNNCC | 2 cut(s) | 29, 255 |
| BspMI | ACCTGC | 1 cut(s) | 733 |
| BspPI | GGATC | 2 cut(s) | 116, 501 |
| BspT107I | GGYRCC | 1 cut(s) | 253 |
| BsrDI | GCAATG | 1 cut(s) | 53 |
| BsrI | ACTGG | 1 cut(s) | 673 |
| BssECI | CCNNGG | 1 cut(s) | 7 |
| BssMI | GATC | 4 cut(s) | 121, 399, 506, 688 |
| BssT1I | CCWWGG | 1 cut(s) | 7 |
| Bst2UI | CCWGG | 1 cut(s) | 63 |
| Bst4CI | ACNGT | 2 cut(s) | 319, 457 |
| Bst6I | CTCTTC | 1 cut(s) | 677 |
| BstC8I | GCNNGC | 2 cut(s) | 447, 730 |
| BstDEI | CTNAG | 1 cut(s) | 224 |
| BstHHI | GCGC | 2 cut(s) | 213, 644 |
| BstKTI | GATC | 4 cut(s) | 124, 402, 509, 691 |
| BstMAI | GTCTC | 1 cut(s) | 563 |
| BstMBI | GATC | 4 cut(s) | 121, 399, 506, 688 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 293 |
| BstNI | CCWGG | 1 cut(s) | 63 |
| BstNSI | RCATGY | 1 cut(s) | 582 |
| BstSCI | CCNGG | 1 cut(s) | 61 |
| BstSFI | CTRYAG | 1 cut(s) | 94 |
| BstV2I | GAAGAC | 1 cut(s) | 227 |
| BstX2I | RGATCY | 1 cut(s) | 506 |
| BstYI | RGATCY | 1 cut(s) | 506 |
| BtsIMutI | CAGTG | 1 cut(s) | 680 |
| BveI | ACCTGC | 1 cut(s) | 733 |
| Cac8I | GCNNGC | 2 cut(s) | 447, 730 |
| CaiI | CAGNNNCTG | 1 cut(s) | 679 |
| CciI | TCATGA | 1 cut(s) | 139 |
| CfoI | GCGC | 2 cut(s) | 213, 644 |
| CseI | GACGC | 1 cut(s) | 776 |
| CviAII | CATG | 6 cut(s) | 116, 140, 208, 579, 656, 733 |
| CviJI | RGCY | 7 cut(s) | 30, 79, 105, 296, 307, 679, 778 |
| CviKI_1 | RGCY | 7 cut(s) | 30, 79, 105, 296, 307, 679, 778 |
| DdeI | CTNAG | 1 cut(s) | 224 |
| DpnI | GATC | 4 cut(s) | 123, 401, 508, 690 |
| DpnII | GATC | 4 cut(s) | 121, 399, 506, 688 |
| Eam1104I | CTCTTC | 1 cut(s) | 677 |
| EarI | CTCTTC | 1 cut(s) | 677 |
| Eco130I | CCWWGG | 1 cut(s) | 7 |
| Eco32I | GATATC | 1 cut(s) | 373 |
| Eco57I | CTGAAG | 1 cut(s) | 701 |
| Eco88I | CYCGRG | 1 cut(s) | 302 |
| EcoRII | CCWGG | 1 cut(s) | 61 |
| EcoRV | GATATC | 1 cut(s) | 373 |
| EcoT14I | CCWWGG | 1 cut(s) | 7 |
| ErhI | CCWWGG | 1 cut(s) | 7 |
| FaeI | CATG | 6 cut(s) | 119, 143, 211, 582, 659, 736 |
| FaqI | GGGAC | 6 cut(s) | 284, 684, 761, 767, 773, 779 |
| FatI | CATG | 6 cut(s) | 115, 139, 207, 578, 655, 732 |
| FblI | GTMKAC | 1 cut(s) | 360 |
| Fnu4HI | GCNGC | 1 cut(s) | 106 |
| Fsp4HI | GCNGC | 1 cut(s) | 106 |
| FspBI | CTAG | 2 cut(s) | 504, 630 |
| GlaI | GCGC | 2 cut(s) | 212, 643 |
| GluI | GCNGC | 1 cut(s) | 106 |
| HgaI | GACGC | 1 cut(s) | 776 |
| HhaI | GCGC | 2 cut(s) | 213, 644 |
| Hin1II | CATG | 6 cut(s) | 119, 143, 211, 582, 659, 736 |
| Hin6I | GCGC | 2 cut(s) | 211, 642 |
| HinP1I | GCGC | 2 cut(s) | 211, 642 |
| HincII | GTYRAC | 1 cut(s) | 361 |
| HindII | GTYRAC | 1 cut(s) | 361 |
| HinfI | GANTC | 3 cut(s) | 259, 537, 571 |
| HphI | GGTGA | 1 cut(s) | 638 |
| Hpy166II | GTNNAC | 2 cut(s) | 361, 635 |
| Hpy188I | TCNGA | 1 cut(s) | 566 |
| Hpy188III | TCNNGA | 5 cut(s) | 140, 185, 263, 368, 504 |
| Hpy8I | GTNNAC | 2 cut(s) | 361, 635 |
| HpyAV | CCTTC | 1 cut(s) | 512 |
| HpyCH4III | ACNGT | 2 cut(s) | 319, 457 |
| HpyCH4V | TGCA | 7 cut(s) | 128, 157, 331, 389, 500, 666, 728 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 293 |
| HpyF3I | CTNAG | 1 cut(s) | 224 |
| Hsp92II | CATG | 6 cut(s) | 119, 143, 211, 582, 659, 736 |
| HspAI | GCGC | 2 cut(s) | 211, 642 |
| Kzo9I | GATC | 4 cut(s) | 121, 399, 506, 688 |
| LmnI | GCTCC | 2 cut(s) | 27, 76 |
| LweI | GCATC | 2 cut(s) | 406, 509 |
| MaeI | CTAG | 2 cut(s) | 504, 630 |
| MaeIII | GTNAC | 3 cut(s) | 178, 216, 480 |
| MalI | GATC | 4 cut(s) | 123, 401, 508, 690 |
| MboI | GATC | 4 cut(s) | 121, 399, 506, 688 |
| MboII | GAAGA | 6 cut(s) | 227, 257, 538, 563, 566, 694 |
| MflI | RGATCY | 1 cut(s) | 506 |
| MluCI | AATT | 3 cut(s) | 281, 339, 409 |
| MlyI | GAGTC | 1 cut(s) | 565 |
| MmeI | TCCRAC | 1 cut(s) | 599 |
| MnlI | CCTC | 4 cut(s) | 6, 259, 520, 733 |
| MseI | TTAA | 3 cut(s) | 81, 194, 408 |
| MslI | CAYNNNNRTG | 2 cut(s) | 440, 654 |
| MspR9I | CCNGG | 1 cut(s) | 63 |
| Mva1269I | GAATGC | 1 cut(s) | 723 |
| MvaI | CCWGG | 1 cut(s) | 63 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 293 |
| NdeII | GATC | 4 cut(s) | 121, 399, 506, 688 |
| NlaIII | CATG | 6 cut(s) | 119, 143, 211, 582, 659, 736 |
| NlaIV | GGNNCC | 2 cut(s) | 29, 255 |
| NmuCI | GTSAC | 2 cut(s) | 178, 480 |
| NspI | RCATGY | 1 cut(s) | 582 |
| PagI | TCATGA | 1 cut(s) | 139 |
| PaqCI | CACCTGC | 1 cut(s) | 733 |
| PciI | ACATGT | 1 cut(s) | 578 |
| PctI | GAATGC | 1 cut(s) | 723 |
| PfeI | GAWTC | 2 cut(s) | 259, 537 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 213 |
| PkrI | GCNGC | 1 cut(s) | 107 |
| PleI | GAGTC | 1 cut(s) | 565 |
| PpsI | GAGTC | 1 cut(s) | 565 |
| PscI | ACATGT | 1 cut(s) | 578 |
| Psp6I | CCWGG | 1 cut(s) | 61 |
| PspGI | CCWGG | 1 cut(s) | 61 |
| PspN4I | GGNNCC | 2 cut(s) | 29, 255 |
| PstNI | CAGNNNCTG | 1 cut(s) | 679 |
| PsuI | RGATCY | 1 cut(s) | 506 |
| RseI | CAYNNNNRTG | 2 cut(s) | 440, 654 |
| SalI | GTCGAC | 1 cut(s) | 359 |
| SaqAI | TTAA | 3 cut(s) | 81, 194, 408 |
| SatI | GCNGC | 1 cut(s) | 106 |
| Sau3AI | GATC | 4 cut(s) | 121, 399, 506, 688 |
| SchI | GAGTC | 1 cut(s) | 565 |
| ScrFI | CCNGG | 1 cut(s) | 63 |
| SetI | ASST | 7 cut(s) | 81, 163, 235, 309, 355, 523, 727 |
| SfaNI | GCATC | 2 cut(s) | 406, 509 |
| SfcI | CTRYAG | 1 cut(s) | 94 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 440, 654 |
| SmlI | CTYRAG | 1 cut(s) | 263 |
| SmoI | CTYRAG | 1 cut(s) | 263 |
| SpeI | ACTAGT | 1 cut(s) | 629 |
| Sse9I | AATT | 3 cut(s) | 281, 339, 409 |
| SsiI | CCGC | 1 cut(s) | 106 |
| SspI | AATATT | 1 cut(s) | 87 |
| SspMI | CTAG | 2 cut(s) | 504, 630 |
| StyD4I | CCNGG | 1 cut(s) | 61 |
| StyI | CCWWGG | 1 cut(s) | 7 |
| TaaI | ACNGT | 2 cut(s) | 319, 457 |
| TaqI | TCGA | 4 cut(s) | 289, 360, 512, 540 |
| TasI | AATT | 3 cut(s) | 281, 339, 409 |
| TauI | GCSGC | 1 cut(s) | 108 |
| TfiI | GAWTC | 2 cut(s) | 259, 537 |
| Tru1I | TTAA | 3 cut(s) | 81, 194, 408 |
| Tru9I | TTAA | 3 cut(s) | 81, 194, 408 |
| TscAI | CASTG | 1 cut(s) | 680 |
| TseFI | GTSAC | 2 cut(s) | 178, 480 |
| Tsp45I | GTSAC | 2 cut(s) | 178, 480 |
| TspDTI | ATGAA | 2 cut(s) | 159, 633 |
| TspRI | CASTG | 1 cut(s) | 680 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 213 |
| XapI | RAATTY | 1 cut(s) | 339 |
| XbaI | TCTAGA | 1 cut(s) | 503 |
| XceI | RCATGY | 1 cut(s) | 582 |
| XmiI | GTMKAC | 1 cut(s) | 360 |
| XspI | CTAG | 2 cut(s) | 504, 630 |