FvH4_6g52530

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000056
Physical Location & Seq
Forward (+)
38649451 .. 38650848
1398 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g52530.t1

Sequence Viewer

Length: 639 bp
ATGGAGTTCACGGAGAAGGAAACTGTTATGAACGCGGTGCAAGAGAGCGTACCGTGCGCGGACAGAGGAGGCGAGGTCGCCGACAATTATATTCTACTCAAATTTTCATTATACAAGTATATCGGCTGCTGCCCTGGTCCGGAGTCCCCAATTCGTTGGGACTCACAAACCCACCAAGGAGAAAAAGACTTCTGGGTCGACCTGGCGGGGATCAAGCAGATGCCTTCCGCCTACCGCAGCCGGTGTAGAAGATCGTTTTCAGATTATCTTTCAACCACGAATGTCCCGGAAGATCAACATGCCCTTGCAATTCAGATGTTACTCGAGCTTGTAGACGAGGCCGTCAACCCTGATCTCCCAATTAACGTGGAGATATTTGACGTGACTGTTCATAATAGGAGGAGGACGATTCCTGCGACAAGGGAGTCGATTGAAGGCTTGGAAAGAGTGAGGCTTGATCTCTTGGAAGAAGATGGAGAAACATGTGCCATTTGTTGGGGGGTTTATGATCAACTGGTTACTAGGTTGCCCTGCTCACACTATTTTCATGGAGAATGTATTTGCGGGTGGCTGCAGATCAATCACGTTTGTCCTATGTGCCGATTCGCAATGCCTACTGAGGAACTGCCATCAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

213

Amino Acids

24.22

Weight (kDa)

4.79

Isoelectric Point (pI)

59.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 161 - 201 2.4e-10 Ring finger domain
zf-C3HC4_2 PF13923 162 - 200 3.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 162 - 200 8.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 162 - 187 1.7e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000056)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74620
fragaria_vesca FvH4_1g17310 FvH4_1g21860 FvH4_2g00301 FvH4_2g00500 FvH4_2g00510 FvH4_2g01040 FvH4_2g01040 FvH4_2g01040 FvH4_2g01511 FvH4_2g01521 FvH4_2g05410 FvH4_2g15860 FvH4_2g16170 FvH4_2g20442 FvH4_3g22600 FvH4_4g03111 FvH4_4g31810 FvH4_4g31840 FvH4_4g33571 FvH4_4g33580 FvH4_4g33600 FvH4_4g33620 FvH4_4g33620 FvH4_5g04820 FvH4_5g04820 FvH4_5g04821 FvH4_5g04821 FvH4_5g04830 FvH4_5g04833 FvH4_5g04833 FvH4_5g04834 FvH4_5g05562 FvH4_5g06150 FvH4_5g07970 FvH4_5g09910 FvH4_5g11280 FvH4_5g12010 FvH4_6g06910 FvH4_6g11580 FvH4_6g15110 FvH4_6g24322 FvH4_6g31360 FvH4_6g34080 FvH4_6g44500 FvH4_6g47060 FvH4_6g50581 FvH4_6g50641 FvH4_6g50650 FvH4_6g50650 FvH4_6g50981 FvH4_6g51090 FvH4_6g51180 FvH4_6g51730 FvH4_6g51740 FvH4_6g51870 FvH4_6g52331 FvH4_6g52340 FvH4_6g52350 FvH4_6g52530 FvH4_6g52530
malus_domestica MD14G1195700.v1.1
prunus_persica Prupe.6G084800_v2.0.a1 Prupe.7G084100_v2.0.a1 Prupe.8G048800_v2.0.a1
pyrus_communis pycom17g01390
rosa_chinensis RchiOBHm_Chr0c11g0499451 RchiOBHm_Chr1g0314791 RchiOBHm_Chr1g0340221 RchiOBHm_Chr1g0362121 RchiOBHm_Chr1g0362131 RchiOBHm_Chr2g0107491 RchiOBHm_Chr2g0127721 RchiOBHm_Chr2g0143901 RchiOBHm_Chr2g0161531 RchiOBHm_Chr2g0169511 RchiOBHm_Chr2g0171151 RchiOBHm_Chr2g0171221 RchiOBHm_Chr2g0171251 RchiOBHm_Chr2g0171261 RchiOBHm_Chr2g0171341 RchiOBHm_Chr2g0171561 RchiOBHm_Chr2g0171591 RchiOBHm_Chr2g0171601 RchiOBHm_Chr2g0172411 RchiOBHm_Chr2g0172441 RchiOBHm_Chr2g0172771 RchiOBHm_Chr2g0172881 RchiOBHm_Chr2g0173201 RchiOBHm_Chr2g0173211 RchiOBHm_Chr2g0173941 RchiOBHm_Chr2g0173951 RchiOBHm_Chr2g0175281 RchiOBHm_Chr3g0463081 RchiOBHm_Chr3g0482941 RchiOBHm_Chr3g0486241 RchiOBHm_Chr3g0488151 RchiOBHm_Chr3g0488191 RchiOBHm_Chr3g0488271 RchiOBHm_Chr3g0488351 RchiOBHm_Chr3g0488411 RchiOBHm_Chr3g0488491 RchiOBHm_Chr4g0410881 RchiOBHm_Chr4g0440431 RchiOBHm_Chr4g0440501 RchiOBHm_Chr6g0269041 RchiOBHm_Chr7g0194201 RchiOBHm_Chr7g0194301 RchiOBHm_Chr7g0194311 RchiOBHm_Chr7g0194391 RchiOBHm_Chr7g0194401 RchiOBHm_Chr7g0236181
rosa_laevigata RLG00000004203 RLG00000006176 RLG00000022170 RLG00000022198 RLG00000022237 RLG00000022911
rosa_multiflora Rmu_co8086672.1_g000001 Rmu_co8091660.1_g000001 Rmu_co8325445.1_g000001 Rmu_co8482323.1_g000001 Rmu_sc0000239.1_g000034 Rmu_sc0000467.1_g000001 Rmu_sc0000766.1_g000038 Rmu_sc0000789.1_g000004 Rmu_sc0000831.1_g000055 Rmu_sc0000831.1_g000056 Rmu_sc0000831.1_g000057 Rmu_sc0000831.1_g000058 Rmu_sc0000831.1_g000059 Rmu_sc0000831.1_g000060 Rmu_sc0000901.1_g000011 Rmu_sc0000901.1_g000029 Rmu_sc0001513.1_g000006 Rmu_sc0001513.1_g000011 Rmu_sc0001576.1_g000034 Rmu_sc0001685.1_g000062 Rmu_sc0002784.1_g000036 Rmu_sc0003188.1_g000026 Rmu_sc0003188.1_g000027 Rmu_sc0003314.1_g000017 Rmu_sc0003548.1_g000019 Rmu_sc0003624.1_g000018 Rmu_sc0005193.1_g000015 Rmu_sc0006328.1_g000001 Rmu_sc0006328.1_g000005 Rmu_sc0006806.1_g000014 Rmu_sc0006806.1_g000028 Rmu_sc0007320.1_g000005 Rmu_sc0007493.1_g000003 Rmu_sc0007646.1_g000011 Rmu_sc0007648.1_g000001 Rmu_sc0007661.1_g000007 Rmu_sc0007684.1_g000008 Rmu_sc0007799.1_g000007 Rmu_sc0007841.1_g000004 Rmu_sc0008051.1_g000003 Rmu_sc0008124.1_g000008 Rmu_sc0009104.1_g000008 Rmu_sc0009433.1_g000012 Rmu_sc0009883.1_g000011 Rmu_sc0010744.1_g000003 Rmu_sc0011052.1_g000003 Rmu_sc0012403.1_g000008 Rmu_sc0013831.1_g000001 Rmu_sc0016754.1_g000004 Rmu_sc0022238.1_g000002 Rmu_sc0023157.1_g000001 Rmu_sc0023894.1_g000001 Rmu_sc0028130.1_g000001 Rmu_sc0040543.1_g000001 Rmu_ssc0000252.1_g000003 Rmu_ssc0000252.1_g000004 Rmu_ssc0000261.1_g000030 Rmu_ssc0000326.1_g000007
rosa_roxburghii Rroxscaffold_157G00438560 Rroxscaffold_2G00078410 Rroxscaffold_2G00078970 Rroxscaffold_2G00079030 Rroxscaffold_2G00079120 Rroxscaffold_2G00079340 Rroxscaffold_2G00079720 Rroxscaffold_2G00079870 Rroxscaffold_2G00079910 Rroxscaffold_2G00080570 Rroxscaffold_2G00080720 Rroxscaffold_2G00080770 Rroxscaffold_2G00080780 Rroxscaffold_2G00080850 Rroxscaffold_2G00080910 Rroxscaffold_2G00116750 Rroxscaffold_3G00261370 Rroxscaffold_3G00261500 Rroxscaffold_3G00266200 Rroxscaffold_4G00305290 Rroxscaffold_4G00311110 Rroxscaffold_4G00327720 Rroxscaffold_5G00381290 Rroxscaffold_5G00381320 Rroxscaffold_6G00394220 Rroxscaffold_6G00416970
rosa_rugosa Rorug01G0164800.1 Rorug01G0164900 Rorug01G0293800 Rorug01G0293900 Rorug01G0294200 Rorug02G0144400 Rorug02G0271300 Rorug02G0271400 Rorug02G0271400 Rorug02G0271500 Rorug02G0384900 Rorug02G0384900 Rorug02G0385000 Rorug02G0385100 Rorug02G0553000 Rorug02G0553100 Rorug02G0555000 Rorug02G0560000 Rorug02G0560100 Rorug02G0561500 Rorug02G0566500 Rorug02G0568800 Rorug02G0569000 Rorug02G0569400 Rorug02G0569700 Rorug02G0575200 Rorug02G0582300 Rorug03G0061300 Rorug03G0126300 Rorug03G0202400 Rorug03G0224200 Rorug03G0238200 Rorug03G0238300 Rorug03G0238400 Rorug03G0255100 Rorug03G0255100 Rorug04G0326000 Rorug04G0326200 Rorug04G0326300 Rorug06G0124000 Rorug07G0015300 Rorug07G0015700 Rorug07G0015700 Rorug07G0265000
rosa_samantha Rh1AG304100 Rh1AG304500 Rh1AG304600 Rh1CG166600 Rh1CG285500 Rh1DG218600 Rh2AG323600 Rh2AG611800 Rh2AG626100 Rh2AG626400 Rh2AG627700 Rh2AG629700 Rh2AG634400 Rh2AG634500 Rh2AG634800 Rh2AG652800 Rh2BG206800 Rh2BG636300 Rh2BG636400 Rh2BG637300 Rh2BG637400 Rh2BG642600 Rh2BG648000 Rh2BG648300 Rh2BG652700 Rh2BG664000 Rh2CG311300 Rh2CG604800 Rh2CG605400 Rh2CG606300 Rh2CG606800 Rh2CG623800 Rh2CG628700 Rh2DG201600 Rh2DG351100 Rh2DG648000 Rh2DG648100 Rh2DG648900 Rh2DG649000 Rh2DG649100 Rh2DG651000 Rh2DG654900 Rh2DG655100 Rh2DG655200 Rh2DG656700 Rh2DG656900 Rh2DG657100 Rh2DG662700 Rh2DG662900 Rh2DG666300 Rh2DG667000 Rh2DG670700 Rh2DG672100 Rh2DG673400 Rh2DG673600 Rh2DG675200 Rh2DG675300 Rh2DG675600 Rh2DG679000 Rh2DG679100 Rh3AG048000 Rh3AG048100 Rh3AG106800 Rh3AG119500 Rh3AG251300 Rh3AG285800 Rh3AG286700 Rh3AG287300 Rh3AG287400 Rh3CG049400 Rh3CG321600 Rh3DG320300 Rh4AG377300 Rh4AG377700 Rh4AG377900 Rh4DG159200 Rh7AG064400 Rh7AG097600 Rh7AG141500 Rh7AG142100 Rh7AG142200 Rh7AG142300 Rh7AG142600 Rh7AG142800 Rh7AG417800 Rh7AG424000 Rh7CG147100 Rh7DG143000 Rh7DG143900 Rh7DG144400 Rh7DG144700
rosa_wichuraiana Rw0G019520 Rw1G014950 Rw1G027080 Rw2G015390 Rw2G035650 Rw2G046060 Rw2G050780 Rw2G050810 Rw2G051810 Rw2G051850 Rw2G051940 Rw2G051990 Rw2G052000 Rw2G052130 Rw2G052140 Rw2G052530 Rw2G052760 Rw2G052830 Rw2G053180 Rw2G053330 Rw2G053630 Rw3G010110 Rw3G024300 Rw3G025400 Rw3G025450 Rw4G032320 Rw4G032440 Rw6G037400 Rw7G012180 Rw7G012220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 194, 341, 424
AccB7I CCANNNNNTGG 1 cut(s) 495
AccI GTMKAC 2 cut(s) 198, 333
AccII CGCG 2 cut(s) 35, 59
AccIII TCCGGA 1 cut(s) 139
AciI CCGC 6 cut(s) 35, 59, 206, 228, 235, 564
AclWI GGATC 1 cut(s) 218
AcsI RAATTY 1 cut(s) 101
AfaI GTAC 1 cut(s) 51
AfiI CCNNNNNNNGG 2 cut(s) 139, 495
AflIII ACRYGT 1 cut(s) 482
AgsI TTSAA 2 cut(s) 273, 434
AjiI CACGTC 1 cut(s) 382
AjnI CCWGG 2 cut(s) 133, 201
AluBI AGCT 1 cut(s) 328
AluI AGCT 1 cut(s) 328
AlwI GGATC 1 cut(s) 218
Ama87I CYCGRG 1 cut(s) 323
Aor13HI TCCGGA 1 cut(s) 139
AoxI GGCC 1 cut(s) 339
ApeKI GCWGC 4 cut(s) 126, 129, 237, 571
ApoI RAATTY 1 cut(s) 101
AspLEI GCGC 1 cut(s) 59
AspS9I GGNCC 1 cut(s) 137
AsuC2I CCSGG 1 cut(s) 287
AvaI CYCGRG 1 cut(s) 323
AvaII GGWCC 1 cut(s) 137
BbvI GCAGC 4 cut(s) 113, 116, 249, 558
BccI CCATC 2 cut(s) 467, 637
BceAI ACGGC 1 cut(s) 326
BciT130I CCWGG 2 cut(s) 135, 203
BclI TGATCA 1 cut(s) 508
BcnI CCSGG 1 cut(s) 287
BfaI CTAG 1 cut(s) 522
BfmI CTRYAG 1 cut(s) 572
BisI GCNGC 4 cut(s) 127, 130, 238, 572
BlsI GCNGC 4 cut(s) 128, 131, 239, 573
Bme1390I CCNGG 3 cut(s) 135, 203, 287
Bme18I GGWCC 1 cut(s) 137
BmeT110I CYCGRG 1 cut(s) 323
BmgBI CACGTC 1 cut(s) 382
BmgT120I GGNCC 1 cut(s) 137
BmrFI CCNGG 3 cut(s) 135, 203, 287
BmsI GCATC 1 cut(s) 210
BpuMI CCSGG 1 cut(s) 287
BsaJI CCNNGG 2 cut(s) 133, 175
BsaWI WCCGGW 1 cut(s) 139
BsaXI ACNNNNNCTCC 2 cut(s) 60, 90
Bsc4I CCNNNNNNNGG 2 cut(s) 139, 495
Bse118I RCCGGY 1 cut(s) 240
Bse1I ACTGG 1 cut(s) 519
Bse3DI GCAATG 1 cut(s) 615
BseAI TCCGGA 1 cut(s) 139
BseBI CCWGG 2 cut(s) 135, 203
BseDI CCNNGG 2 cut(s) 133, 175
BseLI CCNNNNNNNGG 2 cut(s) 139, 495
BseMI GCAATG 1 cut(s) 615
BseMII CTCAG 1 cut(s) 609
BseNI ACTGG 1 cut(s) 519
BseRI GAGGAG 2 cut(s) 81, 415
BseXI GCAGC 4 cut(s) 113, 116, 249, 558
Bsh1236I CGCG 2 cut(s) 35, 59
BshFI GGCC 1 cut(s) 341
BsiHKCI CYCGRG 1 cut(s) 323
BsiSI CCGG 3 cut(s) 140, 241, 287
BslFI GGGAC 3 cut(s) 130, 173, 269
BslI CCNNNNNNNGG 2 cut(s) 139, 495
BsmFI GGGAC 3 cut(s) 130, 173, 269
BsnI GGCC 1 cut(s) 341
BsoBI CYCGRG 1 cut(s) 323
Bsp13I TCCGGA 1 cut(s) 139
Bsp143I GATC 7 cut(s) 210, 251, 292, 352, 457, 508, 576
BspACI CCGC 6 cut(s) 35, 59, 206, 228, 235, 564
BspANI GGCC 1 cut(s) 341
BspCNI CTCAG 1 cut(s) 610
BspEI TCCGGA 1 cut(s) 139
BspFNI CGCG 2 cut(s) 35, 59
BspMAI CTGCAG 1 cut(s) 576
BspPI GGATC 1 cut(s) 218
BsrDI GCAATG 1 cut(s) 615
BsrFI RCCGGY 1 cut(s) 240
BsrI ACTGG 1 cut(s) 519
BssAI RCCGGY 1 cut(s) 240
BssECI CCNNGG 2 cut(s) 133, 175
BssMI GATC 7 cut(s) 210, 251, 292, 352, 457, 508, 576
BssT1I CCWWGG 1 cut(s) 175
Bst2UI CCWGG 2 cut(s) 135, 203
Bst4CI ACNGT 3 cut(s) 25, 54, 388
BstDEI CTNAG 1 cut(s) 618
BstFNI CGCG 2 cut(s) 35, 59
BstHHI GCGC 1 cut(s) 59
BstKTI GATC 7 cut(s) 213, 254, 295, 355, 460, 511, 579
BstMBI GATC 7 cut(s) 210, 251, 292, 352, 457, 508, 576
BstMWI GCNNNNNNNGC 1 cut(s) 54
BstNI CCWGG 2 cut(s) 135, 203
BstNSI RCATGY 2 cut(s) 302, 486
BstSCI CCNGG 3 cut(s) 133, 201, 285
BstSFI CTRYAG 1 cut(s) 572
BstUI CGCG 2 cut(s) 35, 59
BstV1I GCAGC 4 cut(s) 113, 116, 249, 558
BstXI CCANNNNNNTGG 1 cut(s) 156
BsuRI GGCC 1 cut(s) 341
BtrI CACGTC 1 cut(s) 382
CfoI GCGC 1 cut(s) 59
Cfr10I RCCGGY 1 cut(s) 240
Cfr13I GGNCC 1 cut(s) 137
Csp6I GTAC 1 cut(s) 50
CspCI CAANNNNNGTGG 2 cut(s) 348, 383
CviAII CATG 3 cut(s) 299, 483, 548
CviJI RGCY 7 cut(s) 126, 240, 328, 341, 438, 454, 571
CviKI_1 RGCY 7 cut(s) 126, 240, 328, 341, 438, 454, 571
CviQI GTAC 1 cut(s) 50
DdeI CTNAG 1 cut(s) 618
DpnI GATC 7 cut(s) 212, 253, 294, 354, 459, 510, 578
DpnII GATC 7 cut(s) 210, 251, 292, 352, 457, 508, 576
DrdI GACNNNNNNGTC 3 cut(s) 194, 341, 424
DseDI GACNNNNNNGTC 3 cut(s) 194, 341, 424
EciI GGCGGA 1 cut(s) 217
Eco130I CCWWGG 1 cut(s) 175
Eco47I GGWCC 1 cut(s) 137
Eco88I CYCGRG 1 cut(s) 323
EcoRII CCWGG 2 cut(s) 133, 201
EcoT14I CCWWGG 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 175
FaeI CATG 3 cut(s) 302, 486, 551
FaqI GGGAC 3 cut(s) 130, 173, 269
FatI CATG 3 cut(s) 298, 482, 547
FauI CCCGC 2 cut(s) 199, 557
FbaI TGATCA 1 cut(s) 508
FblI GTMKAC 2 cut(s) 198, 333
Fnu4HI GCNGC 4 cut(s) 127, 130, 238, 572
Fsp4HI GCNGC 4 cut(s) 127, 130, 238, 572
FspBI CTAG 1 cut(s) 522
GlaI GCGC 1 cut(s) 58
GluI GCNGC 4 cut(s) 127, 130, 238, 572
HaeIII GGCC 1 cut(s) 341
HapII CCGG 3 cut(s) 140, 241, 287
HhaI GCGC 1 cut(s) 59
Hin1II CATG 3 cut(s) 302, 486, 551
Hin6I GCGC 1 cut(s) 57
HinP1I GCGC 1 cut(s) 57
HincII GTYRAC 2 cut(s) 199, 346
HindII GTYRAC 2 cut(s) 199, 346
HinfI GANTC 5 cut(s) 143, 161, 409, 425, 603
HpaII CCGG 3 cut(s) 140, 241, 287
Hpy166II GTNNAC 4 cut(s) 9, 199, 334, 346
Hpy188I TCNGA 2 cut(s) 262, 315
Hpy188III TCNNGA 1 cut(s) 140
Hpy8I GTNNAC 4 cut(s) 9, 199, 334, 346
HpyAV CCTTC 3 cut(s) 10, 234, 428
HpyCH4III ACNGT 3 cut(s) 25, 54, 388
HpyCH4IV ACGT 3 cut(s) 366, 381, 585
HpyCH4V TGCA 3 cut(s) 40, 308, 574
HpyF10VI GCNNNNNNNGC 1 cut(s) 54
HpyF3I CTNAG 1 cut(s) 618
HpySE526I ACGT 3 cut(s) 366, 381, 585
Hsp92II CATG 3 cut(s) 302, 486, 551
HspAI GCGC 1 cut(s) 57
Kpn2I TCCGGA 1 cut(s) 139
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 7 cut(s) 210, 251, 292, 352, 457, 508, 576
Lsp1109I GCAGC 4 cut(s) 113, 116, 249, 558
LweI GCATC 1 cut(s) 210
MaeI CTAG 1 cut(s) 522
MaeII ACGT 3 cut(s) 366, 381, 585
MaeIII GTNAC 3 cut(s) 318, 382, 517
MalI GATC 7 cut(s) 212, 253, 294, 354, 459, 510, 578
MboI GATC 7 cut(s) 210, 251, 292, 352, 457, 508, 576
MboII GAAGA 4 cut(s) 261, 302, 479, 482
MluCI AATT 5 cut(s) 85, 101, 150, 309, 360
MlyI GAGTC 3 cut(s) 152, 155, 434
MnlI CCTC 8 cut(s) 59, 62, 67, 331, 393, 396, 444, 613
MroI TCCGGA 1 cut(s) 139
MseI TTAA 2 cut(s) 363, 637
MspI CCGG 3 cut(s) 140, 241, 287
MspR9I CCNGG 3 cut(s) 135, 203, 287
MvaI CCWGG 2 cut(s) 135, 203
MvnI CGCG 2 cut(s) 35, 59
MwoI GCNNNNNNNGC 1 cut(s) 54
NciI CCSGG 1 cut(s) 287
NdeII GATC 7 cut(s) 210, 251, 292, 352, 457, 508, 576
NlaIII CATG 3 cut(s) 302, 486, 551
NmuCI GTSAC 1 cut(s) 382
NspI RCATGY 2 cut(s) 302, 486
PaeR7I CTCGAG 1 cut(s) 323
PciI ACATGT 1 cut(s) 482
PcsI WCGNNNNNNNCGW 1 cut(s) 413
PfeI GAWTC 2 cut(s) 409, 603
PflMI CCANNNNNTGG 1 cut(s) 495
PfoI TCCNGGA 1 cut(s) 285
PkrI GCNGC 4 cut(s) 128, 131, 239, 573
PleI GAGTC 3 cut(s) 151, 155, 433
PpsI GAGTC 3 cut(s) 151, 155, 433
PscI ACATGT 1 cut(s) 482
Psp6I CCWGG 2 cut(s) 133, 201
PspGI CCWGG 2 cut(s) 133, 201
PspPI GGNCC 1 cut(s) 137
PspXI VCTCGAGB 1 cut(s) 323
PstI CTGCAG 1 cut(s) 576
RsaI GTAC 1 cut(s) 51
RsaNI GTAC 1 cut(s) 50
SalI GTCGAC 1 cut(s) 197
SaqAI TTAA 2 cut(s) 363, 637
SatI GCNGC 4 cut(s) 127, 130, 238, 572
Sau3AI GATC 7 cut(s) 210, 251, 292, 352, 457, 508, 576
Sau96I GGNCC 1 cut(s) 137
SchI GAGTC 3 cut(s) 152, 155, 434
ScrFI CCNGG 3 cut(s) 135, 203, 287
SetI ASST 7 cut(s) 78, 204, 330, 369, 384, 527, 588
SfaNI GCATC 1 cut(s) 210
SfcI CTRYAG 1 cut(s) 572
Sfr274I CTCGAG 1 cut(s) 323
SinI GGWCC 1 cut(s) 137
SlaI CTCGAG 1 cut(s) 323
SmlI CTYRAG 1 cut(s) 323
SmoI CTYRAG 1 cut(s) 323
Sse9I AATT 5 cut(s) 85, 101, 150, 309, 360
SsiI CCGC 6 cut(s) 35, 59, 206, 228, 235, 564
SspMI CTAG 1 cut(s) 522
StyD4I CCNGG 3 cut(s) 133, 201, 285
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 3 cut(s) 25, 54, 388
TaiI ACGT 3 cut(s) 369, 384, 588
TaqI TCGA 3 cut(s) 198, 324, 428
TasI AATT 5 cut(s) 85, 101, 150, 309, 360
TfiI GAWTC 2 cut(s) 409, 603
Tru1I TTAA 2 cut(s) 363, 637
Tru9I TTAA 2 cut(s) 363, 637
TseFI GTSAC 1 cut(s) 382
TseI GCWGC 4 cut(s) 126, 129, 237, 571
Tsp45I GTSAC 1 cut(s) 382
TspDTI ATGAA 4 cut(s) 44, 96, 380, 536
TspGWI ACGGA 1 cut(s) 26
Van91I CCANNNNNTGG 1 cut(s) 495
VpaK11BI GGWCC 1 cut(s) 137
XapI RAATTY 1 cut(s) 101
XceI RCATGY 2 cut(s) 302, 486
XhoI CTCGAG 1 cut(s) 323
XmiI GTMKAC 2 cut(s) 198, 333
XspI CTAG 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.