ubiquitin-protein transferase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 739 |
| Acc36I | ACCTGC | 1 cut(s) | 739 |
| AccI | GTMKAC | 1 cut(s) | 360 |
| AciI | CCGC | 2 cut(s) | 106, 337 |
| AclWI | GGATC | 2 cut(s) | 116, 507 |
| AcuI | CTGAAG | 1 cut(s) | 707 |
| AfaI | GTAC | 1 cut(s) | 151 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 12 |
| AflIII | ACRYGT | 1 cut(s) | 584 |
| AgsI | TTSAA | 2 cut(s) | 524, 532 |
| AhlI | ACTAGT | 1 cut(s) | 635 |
| AjnI | CCWGG | 1 cut(s) | 61 |
| AleI | CACNNNNGTG | 1 cut(s) | 440 |
| AluBI | AGCT | 2 cut(s) | 79, 307 |
| AluI | AGCT | 2 cut(s) | 79, 307 |
| Alw26I | GTCTC | 1 cut(s) | 569 |
| AlwI | GGATC | 2 cut(s) | 116, 507 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 685 |
| Ama87I | CYCGRG | 1 cut(s) | 302 |
| AspLEI | GCGC | 2 cut(s) | 213, 650 |
| AsuHPI | GGTGA | 1 cut(s) | 644 |
| AvaI | CYCGRG | 1 cut(s) | 302 |
| BbsI | GAAGAC | 1 cut(s) | 227 |
| BccI | CCATC | 1 cut(s) | 793 |
| BciT130I | CCWGG | 1 cut(s) | 63 |
| BcoDI | GTCTC | 1 cut(s) | 569 |
| BcuI | ACTAGT | 1 cut(s) | 635 |
| BfaI | CTAG | 3 cut(s) | 473, 510, 636 |
| BfmI | CTRYAG | 1 cut(s) | 94 |
| BfuAI | ACCTGC | 1 cut(s) | 739 |
| BisI | GCNGC | 1 cut(s) | 106 |
| BlsI | GCNGC | 1 cut(s) | 107 |
| Bme1390I | CCNGG | 1 cut(s) | 63 |
| BmeT110I | CYCGRG | 1 cut(s) | 302 |
| BmiI | GGNNCC | 1 cut(s) | 29 |
| BmrFI | CCNGG | 1 cut(s) | 63 |
| BmsI | GCATC | 2 cut(s) | 406, 515 |
| BpiI | GAAGAC | 1 cut(s) | 227 |
| BpuEI | CTTGAG | 2 cut(s) | 273, 284 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 12 |
| Bse1I | ACTGG | 1 cut(s) | 679 |
| Bse3DI | GCAATG | 1 cut(s) | 53 |
| BseBI | CCWGG | 1 cut(s) | 63 |
| BseGI | GGATG | 1 cut(s) | 333 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 12 |
| BseMI | GCAATG | 1 cut(s) | 53 |
| BseNI | ACTGG | 1 cut(s) | 679 |
| BsgI | GTGCAG | 2 cut(s) | 314, 717 |
| BsiHKCI | CYCGRG | 1 cut(s) | 302 |
| BslFI | GGGAC | 5 cut(s) | 284, 690, 773, 779, 785 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 12 |
| BsmAI | GTCTC | 1 cut(s) | 569 |
| BsmFI | GGGAC | 5 cut(s) | 284, 690, 773, 779, 785 |
| BsmI | GAATGC | 2 cut(s) | 339, 729 |
| BsoBI | CYCGRG | 1 cut(s) | 302 |
| Bsp143I | GATC | 4 cut(s) | 121, 399, 512, 694 |
| BspACI | CCGC | 2 cut(s) | 106, 337 |
| BspLI | GGNNCC | 1 cut(s) | 29 |
| BspMI | ACCTGC | 1 cut(s) | 739 |
| BspPI | GGATC | 2 cut(s) | 116, 507 |
| BsrDI | GCAATG | 1 cut(s) | 53 |
| BsrI | ACTGG | 1 cut(s) | 679 |
| BssMI | GATC | 4 cut(s) | 121, 399, 512, 694 |
| Bst2UI | CCWGG | 1 cut(s) | 63 |
| Bst4CI | ACNGT | 2 cut(s) | 319, 463 |
| Bst6I | CTCTTC | 1 cut(s) | 683 |
| BstC8I | GCNNGC | 1 cut(s) | 453 |
| BstDEI | CTNAG | 1 cut(s) | 224 |
| BstF5I | GGATG | 1 cut(s) | 333 |
| BstHHI | GCGC | 2 cut(s) | 213, 650 |
| BstKTI | GATC | 4 cut(s) | 124, 402, 515, 697 |
| BstMAI | GTCTC | 1 cut(s) | 569 |
| BstMBI | GATC | 4 cut(s) | 121, 399, 512, 694 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 293 |
| BstNI | CCWGG | 1 cut(s) | 63 |
| BstNSI | RCATGY | 4 cut(s) | 211, 234, 455, 588 |
| BstSCI | CCNGG | 1 cut(s) | 61 |
| BstSFI | CTRYAG | 1 cut(s) | 94 |
| BstV2I | GAAGAC | 1 cut(s) | 227 |
| BstX2I | RGATCY | 1 cut(s) | 512 |
| BstYI | RGATCY | 1 cut(s) | 512 |
| BtsCI | GGATG | 1 cut(s) | 333 |
| BtsIMutI | CAGTG | 1 cut(s) | 686 |
| BveI | ACCTGC | 1 cut(s) | 739 |
| Cac8I | GCNNGC | 1 cut(s) | 453 |
| CaiI | CAGNNNCTG | 1 cut(s) | 685 |
| CfoI | GCGC | 2 cut(s) | 213, 650 |
| CseI | GACGC | 1 cut(s) | 782 |
| Csp6I | GTAC | 1 cut(s) | 150 |
| CviAII | CATG | 8 cut(s) | 116, 140, 208, 231, 452, 585, 662, 739 |
| CviJI | RGCY | 7 cut(s) | 30, 79, 105, 296, 307, 685, 784 |
| CviKI_1 | RGCY | 7 cut(s) | 30, 79, 105, 296, 307, 685, 784 |
| CviQI | GTAC | 1 cut(s) | 150 |
| DdeI | CTNAG | 1 cut(s) | 224 |
| DpnI | GATC | 4 cut(s) | 123, 401, 514, 696 |
| DpnII | GATC | 4 cut(s) | 121, 399, 512, 694 |
| Eam1104I | CTCTTC | 1 cut(s) | 683 |
| EarI | CTCTTC | 1 cut(s) | 683 |
| Eco32I | GATATC | 1 cut(s) | 373 |
| Eco57I | CTGAAG | 1 cut(s) | 707 |
| Eco88I | CYCGRG | 1 cut(s) | 302 |
| EcoRII | CCWGG | 1 cut(s) | 61 |
| EcoRV | GATATC | 1 cut(s) | 373 |
| FaeI | CATG | 8 cut(s) | 119, 143, 211, 234, 455, 588, 665, 742 |
| FaqI | GGGAC | 5 cut(s) | 284, 690, 773, 779, 785 |
| FatI | CATG | 8 cut(s) | 115, 139, 207, 230, 451, 584, 661, 738 |
| FblI | GTMKAC | 1 cut(s) | 360 |
| Fnu4HI | GCNGC | 1 cut(s) | 106 |
| FokI | GGATG | 1 cut(s) | 320 |
| Fsp4HI | GCNGC | 1 cut(s) | 106 |
| FspBI | CTAG | 3 cut(s) | 473, 510, 636 |
| GlaI | GCGC | 2 cut(s) | 212, 649 |
| GluI | GCNGC | 1 cut(s) | 106 |
| HgaI | GACGC | 1 cut(s) | 782 |
| HhaI | GCGC | 2 cut(s) | 213, 650 |
| Hin1II | CATG | 8 cut(s) | 119, 143, 211, 234, 455, 588, 665, 742 |
| Hin6I | GCGC | 2 cut(s) | 211, 648 |
| HinP1I | GCGC | 2 cut(s) | 211, 648 |
| HincII | GTYRAC | 1 cut(s) | 361 |
| HindII | GTYRAC | 1 cut(s) | 361 |
| HinfI | GANTC | 3 cut(s) | 259, 543, 577 |
| HphI | GGTGA | 1 cut(s) | 644 |
| Hpy166II | GTNNAC | 2 cut(s) | 361, 641 |
| Hpy188I | TCNGA | 2 cut(s) | 7, 572 |
| Hpy188III | TCNNGA | 4 cut(s) | 185, 263, 368, 510 |
| Hpy8I | GTNNAC | 2 cut(s) | 361, 641 |
| HpyAV | CCTTC | 2 cut(s) | 518, 749 |
| HpyCH4III | ACNGT | 2 cut(s) | 319, 463 |
| HpyCH4V | TGCA | 9 cut(s) | 128, 230, 331, 389, 445, 451, 506, 672, 734 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 293 |
| HpyF3I | CTNAG | 1 cut(s) | 224 |
| Hsp92II | CATG | 8 cut(s) | 119, 143, 211, 234, 455, 588, 665, 742 |
| HspAI | GCGC | 2 cut(s) | 211, 648 |
| Kzo9I | GATC | 4 cut(s) | 121, 399, 512, 694 |
| LmnI | GCTCC | 2 cut(s) | 27, 76 |
| LweI | GCATC | 2 cut(s) | 406, 515 |
| MaeI | CTAG | 3 cut(s) | 473, 510, 636 |
| MaeIII | GTNAC | 3 cut(s) | 178, 216, 486 |
| MalI | GATC | 4 cut(s) | 123, 401, 514, 696 |
| MboI | GATC | 4 cut(s) | 121, 399, 512, 694 |
| MboII | GAAGA | 6 cut(s) | 227, 257, 544, 569, 572, 700 |
| MflI | RGATCY | 1 cut(s) | 512 |
| MluCI | AATT | 2 cut(s) | 281, 409 |
| MlyI | GAGTC | 1 cut(s) | 571 |
| MmeI | TCCRAC | 1 cut(s) | 605 |
| MnlI | CCTC | 4 cut(s) | 6, 259, 526, 739 |
| MseI | TTAA | 3 cut(s) | 81, 194, 408 |
| MslI | CAYNNNNRTG | 3 cut(s) | 440, 450, 660 |
| MspR9I | CCNGG | 1 cut(s) | 63 |
| Mva1269I | GAATGC | 2 cut(s) | 339, 729 |
| MvaI | CCWGG | 1 cut(s) | 63 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 293 |
| NdeII | GATC | 4 cut(s) | 121, 399, 512, 694 |
| NlaIII | CATG | 8 cut(s) | 119, 143, 211, 234, 455, 588, 665, 742 |
| NlaIV | GGNNCC | 1 cut(s) | 29 |
| NmuCI | GTSAC | 2 cut(s) | 178, 486 |
| NspI | RCATGY | 4 cut(s) | 211, 234, 455, 588 |
| OliI | CACNNNNGTG | 1 cut(s) | 440 |
| PaeI | GCATGC | 1 cut(s) | 455 |
| PaqCI | CACCTGC | 1 cut(s) | 739 |
| PciI | ACATGT | 1 cut(s) | 584 |
| PctI | GAATGC | 2 cut(s) | 339, 729 |
| PfeI | GAWTC | 2 cut(s) | 259, 543 |
| PkrI | GCNGC | 1 cut(s) | 107 |
| PleI | GAGTC | 1 cut(s) | 571 |
| PpsI | GAGTC | 1 cut(s) | 571 |
| PscI | ACATGT | 1 cut(s) | 584 |
| Psp6I | CCWGG | 1 cut(s) | 61 |
| PspGI | CCWGG | 1 cut(s) | 61 |
| PspN4I | GGNNCC | 1 cut(s) | 29 |
| PstNI | CAGNNNCTG | 1 cut(s) | 685 |
| PsuI | RGATCY | 1 cut(s) | 512 |
| RsaI | GTAC | 1 cut(s) | 151 |
| RsaNI | GTAC | 1 cut(s) | 150 |
| RseI | CAYNNNNRTG | 3 cut(s) | 440, 450, 660 |
| SalI | GTCGAC | 1 cut(s) | 359 |
| SaqAI | TTAA | 3 cut(s) | 81, 194, 408 |
| SatI | GCNGC | 1 cut(s) | 106 |
| Sau3AI | GATC | 4 cut(s) | 121, 399, 512, 694 |
| SchI | GAGTC | 1 cut(s) | 571 |
| ScrFI | CCNGG | 1 cut(s) | 63 |
| SetI | ASST | 6 cut(s) | 81, 155, 309, 355, 529, 733 |
| SfaNI | GCATC | 2 cut(s) | 406, 515 |
| SfcI | CTRYAG | 1 cut(s) | 94 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 440, 450, 660 |
| SmlI | CTYRAG | 2 cut(s) | 263, 288 |
| SmoI | CTYRAG | 2 cut(s) | 263, 288 |
| SpeI | ACTAGT | 1 cut(s) | 635 |
| SphI | GCATGC | 1 cut(s) | 455 |
| Sse9I | AATT | 2 cut(s) | 281, 409 |
| SsiI | CCGC | 2 cut(s) | 106, 337 |
| SspI | AATATT | 1 cut(s) | 87 |
| SspMI | CTAG | 3 cut(s) | 473, 510, 636 |
| StyD4I | CCNGG | 1 cut(s) | 61 |
| TaaI | ACNGT | 2 cut(s) | 319, 463 |
| TaqI | TCGA | 3 cut(s) | 360, 518, 546 |
| TasI | AATT | 2 cut(s) | 281, 409 |
| TauI | GCSGC | 1 cut(s) | 108 |
| TfiI | GAWTC | 2 cut(s) | 259, 543 |
| Tru1I | TTAA | 3 cut(s) | 81, 194, 408 |
| Tru9I | TTAA | 3 cut(s) | 81, 194, 408 |
| TscAI | CASTG | 1 cut(s) | 686 |
| TseFI | GTSAC | 2 cut(s) | 178, 486 |
| Tsp45I | GTSAC | 2 cut(s) | 178, 486 |
| TspDTI | ATGAA | 2 cut(s) | 159, 639 |
| TspRI | CASTG | 1 cut(s) | 686 |
| XbaI | TCTAGA | 1 cut(s) | 509 |
| XceI | RCATGY | 4 cut(s) | 211, 234, 455, 588 |
| XmiI | GTMKAC | 1 cut(s) | 360 |
| XspI | CTAG | 3 cut(s) | 473, 510, 636 |