ubiquitin-protein transferase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 329 |
| Acc36I | ACCTGC | 1 cut(s) | 351 |
| AciI | CCGC | 3 cut(s) | 114, 118, 223 |
| AclWI | GGATC | 1 cut(s) | 206 |
| AcoI | YGGCCR | 1 cut(s) | 115 |
| AcsI | RAATTY | 1 cut(s) | 89 |
| AfaI | GTAC | 3 cut(s) | 11, 235, 437 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 56 |
| AgsI | TTSAA | 1 cut(s) | 261 |
| AjiI | CACGTC | 1 cut(s) | 325 |
| AjnI | CCWGG | 1 cut(s) | 193 |
| Alw26I | GTCTC | 1 cut(s) | 337 |
| AlwI | GGATC | 1 cut(s) | 206 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 375 |
| AoxI | GGCC | 1 cut(s) | 115 |
| ApeKI | GCWGC | 3 cut(s) | 28, 225, 395 |
| ApoI | RAATTY | 1 cut(s) | 89 |
| AseI | ATTAAT | 1 cut(s) | 242 |
| AspLEI | GCGC | 1 cut(s) | 28 |
| AspS9I | GGNCC | 1 cut(s) | 64 |
| AsuC2I | CCSGG | 1 cut(s) | 275 |
| AvaII | GGWCC | 1 cut(s) | 64 |
| BaeGI | GKGCMC | 1 cut(s) | 45 |
| BbvI | GCAGC | 3 cut(s) | 40, 237, 382 |
| BciT130I | CCWGG | 1 cut(s) | 195 |
| BcnI | CCSGG | 1 cut(s) | 275 |
| BcoDI | GTCTC | 1 cut(s) | 337 |
| BfaI | CTAG | 3 cut(s) | 83, 123, 413 |
| BfuAI | ACCTGC | 1 cut(s) | 351 |
| BisI | GCNGC | 5 cut(s) | 29, 115, 118, 226, 396 |
| BlsI | GCNGC | 5 cut(s) | 30, 116, 119, 227, 397 |
| Bme1390I | CCNGG | 2 cut(s) | 195, 275 |
| Bme18I | GGWCC | 1 cut(s) | 64 |
| BmgBI | CACGTC | 1 cut(s) | 325 |
| BmgT120I | GGNCC | 1 cut(s) | 64 |
| BmrFI | CCNGG | 2 cut(s) | 195, 275 |
| BpuMI | CCSGG | 1 cut(s) | 275 |
| BsaJI | CCNNGG | 3 cut(s) | 21, 193, 194 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 56 |
| Bse1I | ACTGG | 1 cut(s) | 447 |
| BseBI | CCWGG | 1 cut(s) | 195 |
| BseDI | CCNNGG | 3 cut(s) | 21, 193, 194 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 56 |
| BseNI | ACTGG | 1 cut(s) | 447 |
| BseSI | GKGCMC | 1 cut(s) | 45 |
| BseX3I | CGGCCG | 1 cut(s) | 115 |
| BseXI | GCAGC | 3 cut(s) | 40, 237, 382 |
| Bsh1285I | CGRYCG | 1 cut(s) | 118 |
| BshFI | GGCC | 1 cut(s) | 117 |
| BsiEI | CGRYCG | 1 cut(s) | 118 |
| BsiSI | CCGG | 1 cut(s) | 275 |
| BslFI | GGGAC | 2 cut(s) | 161, 257 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 56 |
| BsmAI | GTCTC | 1 cut(s) | 337 |
| BsmFI | GGGAC | 2 cut(s) | 161, 257 |
| BsnI | GGCC | 1 cut(s) | 117 |
| Bsp1286I | GDGCHC | 1 cut(s) | 45 |
| Bsp1407I | TGTACA | 2 cut(s) | 9, 233 |
| Bsp143I | GATC | 1 cut(s) | 198 |
| BspACI | CCGC | 3 cut(s) | 114, 118, 223 |
| BspANI | GGCC | 1 cut(s) | 117 |
| BspMI | ACCTGC | 1 cut(s) | 351 |
| BspPI | GGATC | 1 cut(s) | 206 |
| BsrGI | TGTACA | 2 cut(s) | 9, 233 |
| BsrI | ACTGG | 1 cut(s) | 447 |
| BssECI | CCNNGG | 3 cut(s) | 21, 193, 194 |
| BssMI | GATC | 1 cut(s) | 198 |
| BssT1I | CCWWGG | 1 cut(s) | 21 |
| Bst2UI | CCWGG | 1 cut(s) | 195 |
| BstAUI | TGTACA | 2 cut(s) | 9, 233 |
| BstC8I | GCNNGC | 1 cut(s) | 41 |
| BstHHI | GCGC | 1 cut(s) | 28 |
| BstKTI | GATC | 1 cut(s) | 201 |
| BstMAI | GTCTC | 1 cut(s) | 337 |
| BstMBI | GATC | 1 cut(s) | 198 |
| BstMCI | CGRYCG | 1 cut(s) | 118 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 123, 132, 401 |
| BstNI | CCWGG | 1 cut(s) | 195 |
| BstNSI | RCATGY | 1 cut(s) | 290 |
| BstSCI | CCNGG | 2 cut(s) | 193, 273 |
| BstSLI | GKGCMC | 1 cut(s) | 45 |
| BstV1I | GCAGC | 3 cut(s) | 40, 237, 382 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 144 |
| BstZI | CGGCCG | 1 cut(s) | 115 |
| BsuRI | GGCC | 1 cut(s) | 117 |
| BtgZI | GCGATG | 1 cut(s) | 20 |
| BtrI | CACGTC | 1 cut(s) | 325 |
| BtsIMutI | CAGTG | 1 cut(s) | 454 |
| BveI | ACCTGC | 1 cut(s) | 351 |
| Cac8I | GCNNGC | 1 cut(s) | 41 |
| CaiI | CAGNNNCTG | 1 cut(s) | 375 |
| CciNI | GCGGCCGC | 1 cut(s) | 115 |
| CfoI | GCGC | 1 cut(s) | 28 |
| Cfr13I | GGNCC | 1 cut(s) | 64 |
| Csp6I | GTAC | 3 cut(s) | 10, 234, 436 |
| CviAII | CATG | 1 cut(s) | 287 |
| CviJI | RGCY | 3 cut(s) | 117, 126, 329 |
| CviKI_1 | RGCY | 3 cut(s) | 117, 126, 329 |
| CviQI | GTAC | 3 cut(s) | 10, 234, 436 |
| DpnI | GATC | 1 cut(s) | 200 |
| DpnII | GATC | 1 cut(s) | 198 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 329 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 329 |
| EaeI | YGGCCR | 1 cut(s) | 115 |
| EagI | CGGCCG | 1 cut(s) | 115 |
| EclXI | CGGCCG | 1 cut(s) | 115 |
| Eco130I | CCWWGG | 1 cut(s) | 21 |
| Eco47I | GGWCC | 1 cut(s) | 64 |
| Eco52I | CGGCCG | 1 cut(s) | 115 |
| EcoO109I | RGGNCCY | 1 cut(s) | 64 |
| EcoRII | CCWGG | 1 cut(s) | 193 |
| EcoT14I | CCWWGG | 1 cut(s) | 21 |
| ErhI | CCWWGG | 1 cut(s) | 21 |
| FaeI | CATG | 1 cut(s) | 290 |
| FaiI | YATR | 6 cut(s) | 78, 100, 108, 288, 432, 460 |
| FaqI | GGGAC | 2 cut(s) | 161, 257 |
| FatI | CATG | 1 cut(s) | 286 |
| Fnu4HI | GCNGC | 5 cut(s) | 29, 115, 118, 226, 396 |
| Fsp4HI | GCNGC | 5 cut(s) | 29, 115, 118, 226, 396 |
| FspBI | CTAG | 3 cut(s) | 83, 123, 413 |
| GlaI | GCGC | 1 cut(s) | 27 |
| GluI | GCNGC | 5 cut(s) | 29, 115, 118, 226, 396 |
| HaeIII | GGCC | 1 cut(s) | 117 |
| HapII | CCGG | 1 cut(s) | 275 |
| HhaI | GCGC | 1 cut(s) | 28 |
| Hin1II | CATG | 1 cut(s) | 290 |
| Hin6I | GCGC | 1 cut(s) | 26 |
| HinP1I | GCGC | 1 cut(s) | 26 |
| HinfI | GANTC | 1 cut(s) | 149 |
| HpaII | CCGG | 1 cut(s) | 275 |
| Hpy166II | GTNNAC | 2 cut(s) | 12, 436 |
| Hpy188III | TCNNGA | 1 cut(s) | 320 |
| Hpy8I | GTNNAC | 2 cut(s) | 12, 436 |
| Hpy99I | CGWCG | 1 cut(s) | 74 |
| HpyCH4IV | ACGT | 3 cut(s) | 95, 324, 354 |
| HpyCH4V | TGCA | 1 cut(s) | 398 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 123, 132, 401 |
| HpySE526I | ACGT | 3 cut(s) | 95, 324, 354 |
| Hsp92II | CATG | 1 cut(s) | 290 |
| HspAI | GCGC | 1 cut(s) | 26 |
| Kzo9I | GATC | 1 cut(s) | 198 |
| LpnPI | CCDG | 8 cut(s) | 63, 88, 180, 207, 288, 351, 355, 356 |
| Lsp1109I | GCAGC | 3 cut(s) | 40, 237, 382 |
| MaeI | CTAG | 3 cut(s) | 83, 123, 413 |
| MaeII | ACGT | 3 cut(s) | 95, 324, 354 |
| MaeIII | GTNAC | 1 cut(s) | 208 |
| MalI | GATC | 1 cut(s) | 200 |
| MboI | GATC | 1 cut(s) | 198 |
| MboII | GAAGA | 2 cut(s) | 290, 370 |
| MfeI | CAATTG | 1 cut(s) | 399 |
| MhlI | GDGCHC | 1 cut(s) | 45 |
| MluCI | AATT | 7 cut(s) | 89, 138, 156, 297, 348, 385, 399 |
| MlyI | GAGTC | 1 cut(s) | 143 |
| MnlI | CCTC | 7 cut(s) | 50, 55, 77, 163, 200, 307, 390 |
| MseI | TTAA | 3 cut(s) | 242, 351, 384 |
| MspI | CCGG | 1 cut(s) | 275 |
| MspR9I | CCNGG | 2 cut(s) | 195, 275 |
| MunI | CAATTG | 1 cut(s) | 399 |
| MvaI | CCWGG | 1 cut(s) | 195 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 123, 132, 401 |
| NciI | CCSGG | 1 cut(s) | 275 |
| NdeII | GATC | 1 cut(s) | 198 |
| NlaIII | CATG | 1 cut(s) | 290 |
| NotI | GCGGCCGC | 1 cut(s) | 115 |
| NspI | RCATGY | 1 cut(s) | 290 |
| PasI | CCCWGGG | 1 cut(s) | 194 |
| PfoI | TCCNGGA | 1 cut(s) | 273 |
| PkrI | GCNGC | 5 cut(s) | 30, 116, 119, 227, 397 |
| PleI | GAGTC | 1 cut(s) | 143 |
| PpsI | GAGTC | 1 cut(s) | 143 |
| PpuMI | RGGWCCY | 1 cut(s) | 64 |
| PshBI | ATTAAT | 1 cut(s) | 242 |
| Psp5II | RGGWCCY | 1 cut(s) | 64 |
| Psp6I | CCWGG | 1 cut(s) | 193 |
| PspGI | CCWGG | 1 cut(s) | 193 |
| PspPI | GGNCC | 1 cut(s) | 64 |
| PspPPI | RGGWCCY | 1 cut(s) | 64 |
| PstNI | CAGNNNCTG | 1 cut(s) | 375 |
| RsaI | GTAC | 3 cut(s) | 11, 235, 437 |
| RsaNI | GTAC | 3 cut(s) | 10, 234, 436 |
| SaqAI | TTAA | 3 cut(s) | 242, 351, 384 |
| SatI | GCNGC | 5 cut(s) | 29, 115, 118, 226, 396 |
| Sau3AI | GATC | 1 cut(s) | 198 |
| Sau96I | GGNCC | 1 cut(s) | 64 |
| SchI | GAGTC | 1 cut(s) | 143 |
| ScrFI | CCNGG | 2 cut(s) | 195, 275 |
| SduI | GDGCHC | 1 cut(s) | 45 |
| SinI | GGWCC | 1 cut(s) | 64 |
| Sse9I | AATT | 7 cut(s) | 89, 138, 156, 297, 348, 385, 399 |
| SsiI | CCGC | 3 cut(s) | 114, 118, 223 |
| SspI | AATATT | 1 cut(s) | 179 |
| SspMI | CTAG | 3 cut(s) | 83, 123, 413 |
| StyD4I | CCNGG | 2 cut(s) | 193, 273 |
| StyI | CCWWGG | 1 cut(s) | 21 |
| TaiI | ACGT | 3 cut(s) | 98, 327, 357 |
| TaqI | TCGA | 2 cut(s) | 69, 186 |
| TasI | AATT | 7 cut(s) | 89, 138, 156, 297, 348, 385, 399 |
| TatI | WGTACW | 2 cut(s) | 9, 233 |
| TauI | GCSGC | 2 cut(s) | 117, 120 |
| Tru1I | TTAA | 3 cut(s) | 242, 351, 384 |
| Tru9I | TTAA | 3 cut(s) | 242, 351, 384 |
| TscAI | CASTG | 1 cut(s) | 454 |
| TseI | GCWGC | 3 cut(s) | 28, 225, 395 |
| TspRI | CASTG | 1 cut(s) | 454 |
| VpaK11BI | GGWCC | 1 cut(s) | 64 |
| VspI | ATTAAT | 1 cut(s) | 242 |
| XapI | RAATTY | 1 cut(s) | 89 |
| XceI | RCATGY | 1 cut(s) | 290 |
| XspI | CTAG | 3 cut(s) | 83, 123, 413 |