MD03G1255700.v1.1

Plant transposase (Ptta/En/Spm family)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000260
Physical Location & Seq
Reverse (-)
34296382 .. 34300706
4325 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1255700.v1.1.491

Sequence Viewer

Length: 1458 bp
ATGAGAATATCATGCGCTGTTGAAAGAGAGCATGGCTTGGAGTATTCATTAATCAGGGAAACCTTTCAGATGTCGCGGTTGATCACTCGTCGTAGGAGTGTGACCAATGCGCCTCCCGCATTATCACCATCTACTGCTCCGGCCGTGAGTGCTCCATTGATTGGGGAGCCTACACCTTTCATTGAGGCTACTGCTACGACATCCCAGGTGCCATTATCATCGACGTCATCAGTGTCCGTTCAGTTTCTCAATGCACGGCGGCCTCACCGGCGCCGCCGTGATTCAGAGTCTTCTATTCACAGTTCCTCGTCATCCAGAGTCGAGGATGGGGGCTCCCCTCCAGCTAAAAAAAACACCAGGGGGCCTAATCGAATGCTGAAGGCGGCACAAATGGTACGTCTGTCCGCCTCCTTGATCAAGATTGCATATGACTCGCGACATCGTGGAGCGGCTACCTCACAGCAACATAGTATCGTCGTTACTAGCTGTGGTTATGTTATTAAAAATTGTTGTCCTATGCAATGGAAATCTTGGGCAGAAATTCCCGAGGAGACGAAGATACTGGTGCGAGACAAGTTGTCGGTCAATTTTGATTTTAAGGACATATCCCCCGAGGTCATCACCTACTTAGATGAGACCTTTGCAAACCGGTACAAAAATTGGAAGAGCGATCTTCATGCGCATTTTAAGATATGGGGTGATGTGGAGACTGCTCGCCTACAGGGTTGCCCATCCGAGTTGGCGGACCGGCGAGAGGATTGGGAGTGGCTTTGCAACCATTTTACGGACCCAAAATTTGTGAAGAAATCTATTGCTGGCAAGATTGCTCGGGAGTCAAAGACACTTCTCCACCATTCCGGTTCGAAACCCTTTTCGTATAGGCTTGAGGCACGACGTGAGGAGGGTTCTAAGTTCCCAGAGATCGACGTGTTCAATGACGTTTACGTTCAACCTGGCGATGAGACCAGTGAGCAGCTTTATGCTTCTATGGTGGAAAAGCGTGATGTTGTTCTCCAAGAAGCAACATCGCAACTTCCCCCGGATACCCCGATTGAGGACGTCACTGTATCCGATGATGCAGGTTTTGAGATCATGACTGAGGTCCTGAATCAGAAGTTCGGTCGTCGTCATGGCAAAGTTGTTCGGGGCATGGGGAAGGCGCGTGTTCGTGAAACGGGTGCCTCCTCTTCCAGATCGACCACAGGAGAGGTCAATGCTCTGAAGGAGGAAGTGACAACCTTAAAAGGTCAGCTTGTAGCCCAGAATGAGCAGATGAAGGTTCAGAACGAGCAGTTGAGGGCCGAGAACGAGCAGATGAAGGCTCAGGTTAGGACCCATGACGACAAGATGAATATGATTCTACAGGCCTTAGCGATATCCGGTCTTCAAATCCAGATGCCATCACCTGATCTTGTTCCACCTTCGACTTCCCAGCCATTTCATCCAACTGATACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transposase_24

Protein Analysis

486

Amino Acids

54.39

Weight (kDa)

8.59

Isoelectric Point (pI)

55.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_24 PF03004 252 - 386 1.3e-07 Plant transposase (Ptta/En/Spm family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000260)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01701 FvH4_4g03270
malus_domestica MD00G1017800.v1.1 MD00G1142300.v1.1 MD00G1145500.v1.1 MD00G1191000.v1.1 MD01G1112800.v1.1 MD01G1143700.v1.1 MD02G1048600.v1.1 MD02G1196000.v1.1 MD03G1062700.v1.1 MD03G1095500.v1.1 MD03G1095600.v1.1 MD03G1125500.v1.1 MD03G1154200.v1.1 MD03G1154300.v1.1 MD03G1170400.v1.1 MD03G1179800.v1.1 MD03G1196800.v1.1 MD03G1255700.v1.1 MD04G1029900.v1.1 MD04G1054700.v1.1 MD04G1102500.v1.1 MD04G1142700.v1.1 MD05G1052900.v1.1 MD05G1063000.v1.1 MD05G1069200.v1.1 MD05G1160700.v1.1 MD05G1235500.v1.1 MD05G1285100.v1.1 MD05G1321300.v1.1 MD07G1170900.v1.1 MD08G1072400.v1.1 MD08G1151000.v1.1 MD09G1075100.v1.1 MD11G1004200.v1.1 MD11G1134000.v1.1 MD11G1134100.v1.1 MD11G1150600.v1.1 MD11G1167200.v1.1 MD11G1169500.v1.1 MD11G1191200.v1.1 MD11G1215700.v1.1 MD12G1110400.v1.1 MD12G1226000.v1.1 MD14G1070600.v1.1 MD15G1246900.v1.1 MD15G1271300.v1.1 MD15G1304400.v1.1 MD15G1306500.v1.1 MD15G1394800.v1.1 MD16G1222400.v1.1 MD16G1235600.v1.1 MD16G1275200.v1.1 MD17G1164900.v1.1 MD17G1199400.v1.1 MD17G1200600.v1.1 MD17G1255500.v1.1
prunus_persica Prupe.5G199500_v2.0.a1
pyrus_communis pycom01g05550 pycom01g06510 pycom01g10640 pycom02g04780 pycom02g10380 pycom02g16940 pycom02g21170 pycom02g25250 pycom03g11350 pycom03g15980 pycom03g17500 pycom04g07430 pycom04g11390 pycom05g02890 pycom05g12620 pycom05g21240 pycom06g01740 pycom06g06680 pycom06g08420 pycom07g09890 pycom07g10330 pycom07g13390 pycom07g13400 pycom07g14390 pycom08g04050 pycom08g04060 pycom08g09610 pycom08g14690 pycom09g14670 pycom09g14680 pycom09g19450 pycom10g09940 pycom10g14290 pycom11g03600 pycom11g13710 pycom11g14810 pycom11g17720 pycom12g02240 pycom12g06830 pycom12g09950 pycom12g13060 pycom13g02180 pycom13g07740 pycom13g21900 pycom13g23040 pycom14g10400 pycom14g10410 pycom14g10490 pycom14g10680 pycom14g13340 pycom15g15950 pycom15g31530 pycom15g31550 pycom15g38660 pycom16g08600 pycom16g20570 pycom17g09630 pycom17g09650 pycom17g16800 pycom17g16830 pycom17g24070
rosa_chinensis RchiOBHm_Chr2g0086631
rosa_laevigata RLG00000015768
rosa_multiflora Rmu_co8061662.1_g000001 Rmu_co8118782.1_g000001
rosa_roxburghii Rroxscaffold_2G00154370
rosa_rugosa Rorug01G0468000
rosa_samantha Rh2AG019800 Rh2BG019700 Rh2BG019800 Rh2CG019500 Rh2DG020300
rosa_wichuraiana Rw2G001640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 227, 1062
Acc16I TGCGCA 1 cut(s) 681
Acc36I ACCTGC 1 cut(s) 1070
AccB1I GGYRCC 3 cut(s) 208, 270, 1178
AccB7I CCANNNNNTGG 1 cut(s) 161
AccBSI CCGCTC 1 cut(s) 449
AccII CGCG 3 cut(s) 76, 436, 1162
AciI CCGC 8 cut(s) 76, 117, 259, 274, 383, 405, 449, 743
AcoI YGGCCR 1 cut(s) 141
AcsI RAATTY 2 cut(s) 540, 794
AcuI CTGAAG 2 cut(s) 398, 1241
AcyI GRCGYC 3 cut(s) 224, 271, 1059
AdeI CACNNNGTG 1 cut(s) 896
AfaI GTAC 2 cut(s) 396, 653
AfiI CCNNNNNNNGG 4 cut(s) 161, 754, 784, 1054
AflIII ACRYGT 1 cut(s) 927
AgeI ACCGGT 1 cut(s) 648
AgsI TTSAA 4 cut(s) 23, 934, 950, 1388
AhdI GACNNNNNGTC 1 cut(s) 577
AjiI CACGTC 2 cut(s) 896, 928
AjnI CCWGG 3 cut(s) 204, 356, 952
AluBI AGCT 4 cut(s) 344, 486, 976, 1252
AluI AGCT 4 cut(s) 344, 486, 976, 1252
Alw21I GWGCWC 1 cut(s) 154
Alw26I GTCTC 5 cut(s) 545, 564, 629, 701, 956
Ama87I CYCGRG 3 cut(s) 545, 611, 828
AoxI GGCC 5 cut(s) 141, 260, 362, 1299, 1365
ApeKI GCWGC 1 cut(s) 973
ApoI RAATTY 2 cut(s) 540, 794
ArsI GACNNNNNNTTYG 4 cut(s) 382, 414, 779, 811
AseI ATTAAT 1 cut(s) 50
AsiGI ACCGGT 1 cut(s) 648
Asp700I GAANNNNTTC 1 cut(s) 63
AspLEI GCGC 5 cut(s) 17, 112, 273, 682, 1162
AspS9I GGNCC 6 cut(s) 362, 745, 787, 1102, 1299, 1332
AsuC2I CCSGG 1 cut(s) 1040
AsuHPI GGTGA 5 cut(s) 117, 257, 613, 710, 1395
AsuII TTCGAA 1 cut(s) 863
AvaI CYCGRG 3 cut(s) 545, 611, 828
AvaII GGWCC 4 cut(s) 745, 787, 1102, 1332
BaeI ACNNNNGTAYC 2 cut(s) 386, 419
BanI GGYRCC 3 cut(s) 208, 270, 1178
BanII GRGCYC 1 cut(s) 335
BbsI GAAGAC 2 cut(s) 282, 1376
Bbv12I GWGCWC 1 cut(s) 154
BbvI GCAGC 1 cut(s) 985
BccI CCATC 4 cut(s) 136, 320, 739, 1408
BceAI ACGGC 3 cut(s) 128, 261, 272
BcgI CGANNNNNNTGC 4 cut(s) 414, 448, 659, 693
BciT130I CCWGG 3 cut(s) 206, 358, 954
BciVI GTATCC 2 cut(s) 1036, 1078
BclI TGATCA 2 cut(s) 81, 414
BcnI CCSGG 1 cut(s) 1040
BcoDI GTCTC 5 cut(s) 545, 564, 629, 701, 956
BfaI CTAG 2 cut(s) 483, 1456
BfmI CTRYAG 2 cut(s) 719, 1361
BfoI RGCGCY 1 cut(s) 274
BfuAI ACCTGC 1 cut(s) 1070
BfuI GTATCC 2 cut(s) 1036, 1078
BglI GCCNNNNNGGC 1 cut(s) 268
BisI GCNGC 5 cut(s) 260, 274, 384, 450, 974
BlsI GCNGC 5 cut(s) 261, 275, 385, 451, 975
Bme1390I CCNGG 4 cut(s) 206, 358, 954, 1040
Bme18I GGWCC 4 cut(s) 745, 787, 1102, 1332
BmeRI GACNNNNNGTC 1 cut(s) 577
BmeT110I CYCGRG 3 cut(s) 545, 611, 828
BmgBI CACGTC 2 cut(s) 896, 928
BmgT120I GGNCC 6 cut(s) 362, 745, 787, 1102, 1299, 1332
BmiI GGNNCC 8 cut(s) 168, 210, 272, 334, 363, 789, 1180, 1334
BmrFI CCNGG 4 cut(s) 206, 358, 954, 1040
BmsI GCATC 2 cut(s) 1066, 1386
BoxI GACNNNNGTC 1 cut(s) 1100
BpiI GAAGAC 2 cut(s) 282, 1376
BpmI CTGGAG 1 cut(s) 324
Bpu10I CCTNAGC 2 cut(s) 1323, 1369
Bpu14I TTCGAA 1 cut(s) 863
BpuEI CTTGAG 1 cut(s) 905
BpuMI CCSGG 1 cut(s) 1040
BsaHI GRCGYC 3 cut(s) 224, 271, 1059
BsaI GGTCTC 2 cut(s) 629, 956
BsaJI CCNNGG 5 cut(s) 204, 357, 546, 612, 1038
BsaWI WCCGGW 3 cut(s) 648, 857, 1379
Bsc4I CCNNNNNNNGG 4 cut(s) 161, 754, 784, 1054
Bse118I RCCGGY 3 cut(s) 267, 648, 747
Bse1I ACTGG 2 cut(s) 567, 966
Bse3DI GCAATG 1 cut(s) 527
BseBI CCWGG 3 cut(s) 206, 358, 954
BseDI CCNNGG 5 cut(s) 204, 357, 546, 612, 1038
BseGI GGATG 5 cut(s) 200, 311, 331, 731, 1441
BseLI CCNNNNNNNGG 4 cut(s) 161, 754, 784, 1054
BseMI GCAATG 1 cut(s) 527
BseMII CTCAG 2 cut(s) 1089, 1337
BseNI ACTGG 2 cut(s) 567, 966
BseRI GAGGAG 3 cut(s) 563, 914, 1174
BseX3I CGGCCG 1 cut(s) 141
BseXI GCAGC 1 cut(s) 985
BseYI CCCAGC 1 cut(s) 1431
Bsh1236I CGCG 3 cut(s) 76, 436, 1162
Bsh1285I CGRYCG 2 cut(s) 144, 1123
BshFI GGCC 5 cut(s) 143, 262, 364, 1301, 1367
BshNI GGYRCC 3 cut(s) 208, 270, 1178
BshTI ACCGGT 1 cut(s) 648
BsiEI CGRYCG 2 cut(s) 144, 1123
BsiHKAI GWGCWC 1 cut(s) 154
BsiHKCI CYCGRG 3 cut(s) 545, 611, 828
BsiSI CCGG 7 cut(s) 140, 268, 649, 748, 858, 1040, 1380
BslI CCNNNNNNNGG 4 cut(s) 161, 754, 784, 1054
BsmAI GTCTC 5 cut(s) 545, 564, 629, 701, 956
BsmBI CGTCTC 1 cut(s) 545
BsmI GAATGC 1 cut(s) 378
BsnI GGCC 5 cut(s) 143, 262, 364, 1301, 1367
Bso31I GGTCTC 2 cut(s) 629, 956
BsoBI CYCGRG 3 cut(s) 545, 611, 828
Bsp119I TTCGAA 1 cut(s) 863
Bsp1286I GDGCHC 2 cut(s) 154, 335
Bsp143I GATC 7 cut(s) 81, 414, 670, 921, 1089, 1193, 1408
Bsp68I TCGCGA 1 cut(s) 436
BspACI CCGC 8 cut(s) 76, 117, 259, 274, 383, 405, 449, 743
BspANI GGCC 5 cut(s) 143, 262, 364, 1301, 1367
BspCNI CTCAG 2 cut(s) 1090, 1336
BspFNI CGCG 3 cut(s) 76, 436, 1162
BspHI TCATGA 1 cut(s) 1092
BspLI GGNNCC 8 cut(s) 168, 210, 272, 334, 363, 789, 1180, 1334
BspMI ACCTGC 1 cut(s) 1070
BspQI GCTCTTC 1 cut(s) 659
BspT104I TTCGAA 1 cut(s) 863
BspT107I GGYRCC 3 cut(s) 208, 270, 1178
BspTNI GGTCTC 2 cut(s) 629, 956
BsrBI CCGCTC 1 cut(s) 449
BsrDI GCAATG 1 cut(s) 527
BsrFI RCCGGY 3 cut(s) 267, 648, 747
BsrI ACTGG 2 cut(s) 567, 966
BssAI RCCGGY 3 cut(s) 267, 648, 747
BssECI CCNNGG 5 cut(s) 204, 357, 546, 612, 1038
BssMI GATC 7 cut(s) 81, 414, 670, 921, 1089, 1193, 1408
BssNI GRCGYC 3 cut(s) 224, 271, 1059
Bst2UI CCWGG 3 cut(s) 206, 358, 954
Bst4CI ACNGT 2 cut(s) 302, 1066
Bst6I CTCTTC 2 cut(s) 659, 1192
BstACI GRCGYC 3 cut(s) 224, 271, 1059
BstBI TTCGAA 1 cut(s) 863
BstC8I GCNNGC 2 cut(s) 715, 817
BstDEI CTNAG 5 cut(s) 628, 909, 1098, 1323, 1369
BstF5I GGATG 5 cut(s) 200, 311, 331, 731, 1441
BstFNI CGCG 3 cut(s) 76, 436, 1162
BstH2I RGCGCY 1 cut(s) 274
BstHHI GCGC 5 cut(s) 17, 112, 273, 682, 1162
BstKTI GATC 7 cut(s) 84, 417, 673, 924, 1092, 1196, 1411
BstMAI GTCTC 5 cut(s) 545, 564, 629, 701, 956
BstMBI GATC 7 cut(s) 81, 414, 670, 921, 1089, 1193, 1408
BstMCI CGRYCG 2 cut(s) 144, 1123
BstMWI GCNNNNNNNGC 3 cut(s) 116, 149, 268
BstNI CCWGG 3 cut(s) 206, 358, 954
BstPAI GACNNNNGTC 1 cut(s) 1100
BstSCI CCNGG 4 cut(s) 204, 356, 952, 1038
BstSFI CTRYAG 2 cut(s) 719, 1361
BstUI CGCG 3 cut(s) 76, 436, 1162
BstV1I GCAGC 1 cut(s) 985
BstV2I GAAGAC 2 cut(s) 282, 1376
BstZI CGGCCG 1 cut(s) 141
BsuI GTATCC 2 cut(s) 1036, 1078
BsuRI GGCC 5 cut(s) 143, 262, 364, 1301, 1367
BtgZI GCGATG 2 cut(s) 972, 1011
BtrI CACGTC 2 cut(s) 896, 928
BtsCI GGATG 5 cut(s) 200, 311, 331, 731, 1441
BtsIMutI CAGTG 3 cut(s) 237, 973, 1062
BtuMI TCGCGA 1 cut(s) 436
BveI ACCTGC 1 cut(s) 1070
Cac8I GCNNGC 2 cut(s) 715, 817
CciI TCATGA 1 cut(s) 1092
CfoI GCGC 5 cut(s) 17, 112, 273, 682, 1162
Cfr10I RCCGGY 3 cut(s) 267, 648, 747
Cfr13I GGNCC 6 cut(s) 362, 745, 787, 1102, 1299, 1332
CpoI CGGWCCG 1 cut(s) 745
Csp6I GTAC 2 cut(s) 395, 652
CspAI ACCGGT 1 cut(s) 648
CspI CGGWCCG 1 cut(s) 745
CviAII CATG 7 cut(s) 12, 32, 677, 1093, 1130, 1150, 1337
CviQI GTAC 2 cut(s) 395, 652
DdeI CTNAG 5 cut(s) 628, 909, 1098, 1323, 1369
DinI GGCGCC 1 cut(s) 272
DpnI GATC 7 cut(s) 83, 416, 672, 923, 1091, 1195, 1410
DpnII GATC 7 cut(s) 81, 414, 670, 921, 1089, 1193, 1408
DraIII CACNNNGTG 1 cut(s) 896
DriI GACNNNNNGTC 1 cut(s) 577
EaeI YGGCCR 1 cut(s) 141
EagI CGGCCG 1 cut(s) 141
Eam1104I CTCTTC 2 cut(s) 659, 1192
Eam1105I GACNNNNNGTC 1 cut(s) 577
EarI CTCTTC 2 cut(s) 659, 1192
EciI GGCGGA 2 cut(s) 394, 758
EclXI CGGCCG 1 cut(s) 141
Eco147I AGGCCT 1 cut(s) 1367
Eco24I GRGCYC 1 cut(s) 335
Eco31I GGTCTC 2 cut(s) 629, 956
Eco32I GATATC 1 cut(s) 1377
Eco47I GGWCC 4 cut(s) 745, 787, 1102, 1332
Eco52I CGGCCG 1 cut(s) 141
Eco57I CTGAAG 2 cut(s) 398, 1241
Eco88I CYCGRG 3 cut(s) 545, 611, 828
EcoO109I RGGNCCY 3 cut(s) 362, 1102, 1332
EcoRII CCWGG 3 cut(s) 204, 356, 952
EcoRV GATATC 1 cut(s) 1377
EcoT38I GRGCYC 1 cut(s) 335
EgeI GGCGCC 1 cut(s) 272
EheI GGCGCC 1 cut(s) 272
Esp3I CGTCTC 1 cut(s) 545
FaeI CATG 7 cut(s) 15, 35, 680, 1096, 1133, 1153, 1340
FalI AAGNNNNNCTT 2 cut(s) 1236, 1268
FatI CATG 7 cut(s) 11, 31, 676, 1092, 1129, 1149, 1336
FauI CCCGC 1 cut(s) 124
FauNDI CATATG 1 cut(s) 427
FbaI TGATCA 2 cut(s) 81, 414
Fnu4HI GCNGC 5 cut(s) 260, 274, 384, 450, 974
FokI GGATG 5 cut(s) 187, 298, 338, 718, 1428
FriOI GRGCYC 1 cut(s) 335
Fsp4HI GCNGC 5 cut(s) 260, 274, 384, 450, 974
FspAI RTGCGCAY 1 cut(s) 681
FspBI CTAG 2 cut(s) 483, 1456
FspI TGCGCA 1 cut(s) 681
GlaI GCGC 5 cut(s) 16, 111, 272, 681, 1161
GluI GCNGC 5 cut(s) 260, 274, 384, 450, 974
GsaI CCCAGC 1 cut(s) 1435
GsuI CTGGAG 1 cut(s) 324
HaeII RGCGCY 1 cut(s) 274
HaeIII GGCC 5 cut(s) 143, 262, 364, 1301, 1367
HapII CCGG 7 cut(s) 140, 268, 649, 748, 858, 1040, 1380
HhaI GCGC 5 cut(s) 17, 112, 273, 682, 1162
Hin1I GRCGYC 3 cut(s) 224, 271, 1059
Hin1II CATG 7 cut(s) 15, 35, 680, 1096, 1133, 1153, 1340
Hin6I GCGC 5 cut(s) 15, 110, 271, 680, 1160
HinP1I GCGC 5 cut(s) 15, 110, 271, 680, 1160
HinfI GANTC 7 cut(s) 281, 287, 318, 431, 833, 1108, 1357
HpaII CCGG 7 cut(s) 140, 268, 649, 748, 858, 1040, 1380
HphI GGTGA 5 cut(s) 117, 257, 613, 710, 1395
Hpy166II GTNNAC 1 cut(s) 943
Hpy188I TCNGA 7 cut(s) 69, 286, 736, 1072, 1113, 1221, 1284
Hpy8I GTNNAC 1 cut(s) 943
Hpy99I CGWCG 6 cut(s) 93, 226, 479, 897, 929, 1128
HpyAV CCTTC 6 cut(s) 373, 1150, 1216, 1270, 1312, 1431
HpyCH4III ACNGT 2 cut(s) 302, 1066
HpyCH4IV ACGT 7 cut(s) 224, 397, 895, 927, 939, 945, 1059
HpyCH4V TGCA 6 cut(s) 254, 425, 520, 644, 774, 1079
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 149, 268
HpyF3I CTNAG 5 cut(s) 628, 909, 1098, 1323, 1369
HpySE526I ACGT 7 cut(s) 224, 397, 895, 927, 939, 945, 1059
Hsp92I GRCGYC 3 cut(s) 224, 271, 1059
Hsp92II CATG 7 cut(s) 15, 35, 680, 1096, 1133, 1153, 1340
HspAI GCGC 5 cut(s) 15, 110, 271, 680, 1160
KasI GGCGCC 1 cut(s) 270
Ksp22I TGATCA 2 cut(s) 81, 414
Kzo9I GATC 7 cut(s) 81, 414, 670, 921, 1089, 1193, 1408
LguI GCTCTTC 1 cut(s) 659
LmnI GCTCC 5 cut(s) 142, 157, 166, 338, 446
Lsp1109I GCAGC 1 cut(s) 985
LweI GCATC 2 cut(s) 1066, 1386
MaeI CTAG 2 cut(s) 483, 1456
MaeII ACGT 7 cut(s) 224, 397, 895, 927, 939, 945, 1059
MaeIII GTNAC 4 cut(s) 100, 478, 1060, 1231
MalI GATC 7 cut(s) 83, 416, 672, 923, 1091, 1195, 1410
MbiI CCGCTC 1 cut(s) 449
MboI GATC 7 cut(s) 81, 414, 670, 921, 1089, 1193, 1408
MboII GAAGA 7 cut(s) 282, 568, 665, 676, 814, 1179, 1376
MhlI GDGCHC 2 cut(s) 154, 335
MluCI AATT 5 cut(s) 505, 540, 586, 658, 794
Mly113I GGCGCC 1 cut(s) 271
MlyI GAGTC 4 cut(s) 296, 327, 425, 842
MroXI GAANNNNTTC 1 cut(s) 63
MseI TTAA 5 cut(s) 50, 501, 597, 687, 1241
MspI CCGG 7 cut(s) 140, 268, 649, 748, 858, 1040, 1380
MspR9I CCNGG 4 cut(s) 206, 358, 954, 1040
Mva1269I GAATGC 1 cut(s) 378
MvaI CCWGG 3 cut(s) 206, 358, 954
MvnI CGCG 3 cut(s) 76, 436, 1162
MwoI GCNNNNNNNGC 3 cut(s) 116, 149, 268
NarI GGCGCC 1 cut(s) 271
NciI CCSGG 1 cut(s) 1040
NdeI CATATG 1 cut(s) 427
NdeII GATC 7 cut(s) 81, 414, 670, 921, 1089, 1193, 1408
NlaIII CATG 7 cut(s) 15, 35, 680, 1096, 1133, 1153, 1340
NlaIV GGNNCC 8 cut(s) 168, 210, 272, 334, 363, 789, 1180, 1334
NmeAIII GCCGAG 1 cut(s) 1327
NmuCI GTSAC 3 cut(s) 100, 1060, 1231
NruI TCGCGA 1 cut(s) 436
NsbI TGCGCA 1 cut(s) 681
NspV TTCGAA 1 cut(s) 863
PagI TCATGA 1 cut(s) 1092
PceI AGGCCT 1 cut(s) 1367
PciSI GCTCTTC 1 cut(s) 659
PctI GAATGC 1 cut(s) 378
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 3 cut(s) 281, 1108, 1357
PflMI CCANNNNNTGG 1 cut(s) 161
PinAI ACCGGT 1 cut(s) 648
PkrI GCNGC 5 cut(s) 261, 275, 385, 451, 975
PleI GAGTC 4 cut(s) 295, 326, 425, 841
PluTI GGCGCC 1 cut(s) 274
PpsI GAGTC 4 cut(s) 295, 326, 425, 841
PpuMI RGGWCCY 2 cut(s) 1102, 1332
PshAI GACNNNNGTC 1 cut(s) 1100
PshBI ATTAAT 1 cut(s) 50
Psp5II RGGWCCY 2 cut(s) 1102, 1332
Psp6I CCWGG 3 cut(s) 204, 356, 952
PspFI CCCAGC 1 cut(s) 1431
PspGI CCWGG 3 cut(s) 204, 356, 952
PspN4I GGNNCC 8 cut(s) 168, 210, 272, 334, 363, 789, 1180, 1334
PspPI GGNCC 6 cut(s) 362, 745, 787, 1102, 1299, 1332
PspPPI RGGWCCY 2 cut(s) 1102, 1332
RruI TCGCGA 1 cut(s) 436
RsaI GTAC 2 cut(s) 396, 653
RsaNI GTAC 2 cut(s) 395, 652
Rsr2I CGGWCCG 1 cut(s) 745
RsrII CGGWCCG 1 cut(s) 745
SapI GCTCTTC 1 cut(s) 659
SaqAI TTAA 5 cut(s) 50, 501, 597, 687, 1241
SatI GCNGC 5 cut(s) 260, 274, 384, 450, 974
Sau3AI GATC 7 cut(s) 81, 414, 670, 921, 1089, 1193, 1408
Sau96I GGNCC 6 cut(s) 362, 745, 787, 1102, 1299, 1332
SchI GAGTC 4 cut(s) 296, 327, 425, 842
ScrFI CCNGG 4 cut(s) 206, 358, 954, 1040
SduI GDGCHC 2 cut(s) 154, 335
SfaNI GCATC 2 cut(s) 1066, 1386
SfcI CTRYAG 2 cut(s) 719, 1361
SfoI GGCGCC 1 cut(s) 272
SfuI TTCGAA 1 cut(s) 863
SgrAI CRCCGGYG 1 cut(s) 267
SinI GGWCC 4 cut(s) 745, 787, 1102, 1332
SmlI CTYRAG 1 cut(s) 884
SmoI CTYRAG 1 cut(s) 884
Sse9I AATT 5 cut(s) 505, 540, 586, 658, 794
SseBI AGGCCT 1 cut(s) 1367
SsiI CCGC 8 cut(s) 76, 117, 259, 274, 383, 405, 449, 743
SspDI GGCGCC 1 cut(s) 270
SspMI CTAG 2 cut(s) 483, 1456
StuI AGGCCT 1 cut(s) 1367
StyD4I CCNGG 4 cut(s) 204, 356, 952, 1038
TaaI ACNGT 2 cut(s) 302, 1066
TaiI ACGT 7 cut(s) 227, 400, 898, 930, 942, 948, 1062
TaqI TCGA 7 cut(s) 221, 321, 370, 863, 924, 1196, 1424
TaqII GACCGA 2 cut(s) 571, 1109
TasI AATT 5 cut(s) 505, 540, 586, 658, 794
TauI GCSGC 4 cut(s) 262, 276, 386, 452
TfiI GAWTC 3 cut(s) 281, 1108, 1357
Tru1I TTAA 5 cut(s) 50, 501, 597, 687, 1241
Tru9I TTAA 5 cut(s) 50, 501, 597, 687, 1241
TscAI CASTG 3 cut(s) 237, 973, 1069
TseFI GTSAC 3 cut(s) 100, 1060, 1231
TseI GCWGC 1 cut(s) 973
Tsp45I GTSAC 3 cut(s) 100, 1060, 1231
TspDTI ATGAA 7 cut(s) 36, 169, 665, 1289, 1331, 1364, 1430
TspGWI ACGGA 2 cut(s) 226, 800
TspRI CASTG 3 cut(s) 237, 973, 1069
Van91I CCANNNNNTGG 1 cut(s) 161
VpaK11BI GGWCC 4 cut(s) 745, 787, 1102, 1332
VspI ATTAAT 1 cut(s) 50
XapI RAATTY 2 cut(s) 540, 794
XmnI GAANNNNTTC 1 cut(s) 63
XspI CTAG 2 cut(s) 483, 1456
ZraI GACGTC 2 cut(s) 225, 1060
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.