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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 16 |
| AclWI | GGATC | 1 cut(s) | 32 |
| AcsI | RAATTY | 2 cut(s) | 230, 249 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 175 |
| Alw26I | GTCTC | 2 cut(s) | 24, 54 |
| AlwI | GGATC | 1 cut(s) | 32 |
| AoxI | GGCC | 1 cut(s) | 119 |
| ApoI | RAATTY | 2 cut(s) | 230, 249 |
| AspLEI | GCGC | 1 cut(s) | 101 |
| AspS9I | GGNCC | 2 cut(s) | 120, 180 |
| AsuHPI | GGTGA | 1 cut(s) | 91 |
| AvaII | GGWCC | 1 cut(s) | 180 |
| BaeGI | GKGCMC | 1 cut(s) | 8 |
| BceAI | ACGGC | 1 cut(s) | 134 |
| BcoDI | GTCTC | 2 cut(s) | 24, 54 |
| BfaI | CTAG | 1 cut(s) | 150 |
| BfoI | RGCGCY | 1 cut(s) | 102 |
| BlpI | GCTNAGC | 1 cut(s) | 141 |
| Bme18I | GGWCC | 1 cut(s) | 180 |
| BmgT120I | GGNCC | 2 cut(s) | 120, 180 |
| BmiI | GGNNCC | 3 cut(s) | 57, 122, 181 |
| BmsI | GCATC | 1 cut(s) | 94 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 36 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 141 |
| BsaJI | CCNNGG | 1 cut(s) | 169 |
| BsaWI | WCCGGW | 1 cut(s) | 22 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 175 |
| BseDI | CCNNGG | 1 cut(s) | 169 |
| BseGI | GGATG | 1 cut(s) | 85 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 175 |
| BseMII | CTCAG | 2 cut(s) | 109, 132 |
| BseSI | GKGCMC | 1 cut(s) | 8 |
| BshFI | GGCC | 1 cut(s) | 121 |
| BsiSI | CCGG | 1 cut(s) | 23 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 175 |
| BsmAI | GTCTC | 2 cut(s) | 24, 54 |
| BsnI | GGCC | 1 cut(s) | 121 |
| Bsp1286I | GDGCHC | 1 cut(s) | 8 |
| Bsp143I | GATC | 1 cut(s) | 37 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 141 |
| Bsp19I | CCATGG | 1 cut(s) | 169 |
| BspANI | GGCC | 1 cut(s) | 121 |
| BspCNI | CTCAG | 2 cut(s) | 108, 133 |
| BspLI | GGNNCC | 3 cut(s) | 57, 122, 181 |
| BspPI | GGATC | 1 cut(s) | 32 |
| BssECI | CCNNGG | 1 cut(s) | 169 |
| BssMI | GATC | 1 cut(s) | 37 |
| BssT1I | CCWWGG | 1 cut(s) | 169 |
| Bst4CI | ACNGT | 1 cut(s) | 46 |
| BstDEI | CTNAG | 3 cut(s) | 95, 132, 141 |
| BstDSI | CCRYGG | 1 cut(s) | 169 |
| BstF5I | GGATG | 1 cut(s) | 85 |
| BstH2I | RGCGCY | 1 cut(s) | 102 |
| BstHHI | GCGC | 1 cut(s) | 101 |
| BstKTI | GATC | 1 cut(s) | 40 |
| BstMAI | GTCTC | 2 cut(s) | 24, 54 |
| BstMBI | GATC | 1 cut(s) | 37 |
| BstSLI | GKGCMC | 1 cut(s) | 8 |
| BsuRI | GGCC | 1 cut(s) | 121 |
| BtgI | CCRYGG | 1 cut(s) | 169 |
| BtsCI | GGATG | 1 cut(s) | 85 |
| BtsIMutI | CAGTG | 1 cut(s) | 51 |
| CfoI | GCGC | 1 cut(s) | 101 |
| Cfr13I | GGNCC | 2 cut(s) | 120, 180 |
| CseI | GACGC | 1 cut(s) | 91 |
| CviAII | CATG | 1 cut(s) | 170 |
| CviJI | RGCY | 4 cut(s) | 56, 121, 140, 158 |
| CviKI_1 | RGCY | 4 cut(s) | 56, 121, 140, 158 |
| DdeI | CTNAG | 3 cut(s) | 95, 132, 141 |
| DpnI | GATC | 1 cut(s) | 39 |
| DpnII | GATC | 1 cut(s) | 37 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 16 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 16 |
| Eco130I | CCWWGG | 1 cut(s) | 169 |
| Eco47I | GGWCC | 1 cut(s) | 180 |
| EcoO109I | RGGNCCY | 1 cut(s) | 180 |
| EcoT14I | CCWWGG | 1 cut(s) | 169 |
| ErhI | CCWWGG | 1 cut(s) | 169 |
| FaeI | CATG | 1 cut(s) | 173 |
| FaiI | YATR | 4 cut(s) | 171, 186, 207, 260 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 146, 178 |
| FatI | CATG | 1 cut(s) | 169 |
| FokI | GGATG | 1 cut(s) | 72 |
| FspBI | CTAG | 1 cut(s) | 150 |
| GlaI | GCGC | 1 cut(s) | 100 |
| HaeII | RGCGCY | 1 cut(s) | 102 |
| HaeIII | GGCC | 1 cut(s) | 121 |
| HapII | CCGG | 1 cut(s) | 23 |
| HgaI | GACGC | 1 cut(s) | 91 |
| HhaI | GCGC | 1 cut(s) | 101 |
| Hin1II | CATG | 1 cut(s) | 173 |
| Hin6I | GCGC | 1 cut(s) | 99 |
| HinP1I | GCGC | 1 cut(s) | 99 |
| HinfI | GANTC | 1 cut(s) | 109 |
| HpaII | CCGG | 1 cut(s) | 23 |
| HphI | GGTGA | 1 cut(s) | 91 |
| Hpy99I | CGWCG | 1 cut(s) | 13 |
| HpyCH4III | ACNGT | 1 cut(s) | 46 |
| HpyF3I | CTNAG | 3 cut(s) | 95, 132, 141 |
| Hsp92II | CATG | 1 cut(s) | 173 |
| HspAI | GCGC | 1 cut(s) | 99 |
| Kzo9I | GATC | 1 cut(s) | 37 |
| LmnI | GCTCC | 1 cut(s) | 61 |
| LpnPI | CCDG | 4 cut(s) | 36, 38, 137, 174 |
| LweI | GCATC | 1 cut(s) | 94 |
| MaeI | CTAG | 1 cut(s) | 150 |
| MaeIII | GTNAC | 2 cut(s) | 25, 79 |
| MalI | GATC | 1 cut(s) | 39 |
| MboI | GATC | 1 cut(s) | 37 |
| MboII | GAAGA | 2 cut(s) | 57, 66 |
| MhlI | GDGCHC | 1 cut(s) | 8 |
| MluCI | AATT | 2 cut(s) | 230, 249 |
| MlyI | GAGTC | 1 cut(s) | 103 |
| MmeI | TCCRAC | 1 cut(s) | 65 |
| MnlI | CCTC | 4 cut(s) | 99, 130, 169, 212 |
| MspI | CCGG | 1 cut(s) | 23 |
| NcoI | CCATGG | 1 cut(s) | 169 |
| NdeII | GATC | 1 cut(s) | 37 |
| NlaIII | CATG | 1 cut(s) | 173 |
| NlaIV | GGNNCC | 3 cut(s) | 57, 122, 181 |
| NmuCI | GTSAC | 2 cut(s) | 25, 79 |
| PleI | GAGTC | 1 cut(s) | 103 |
| PpsI | GAGTC | 1 cut(s) | 103 |
| PpuMI | RGGWCCY | 1 cut(s) | 180 |
| Psp5II | RGGWCCY | 1 cut(s) | 180 |
| PspN4I | GGNNCC | 3 cut(s) | 57, 122, 181 |
| PspPI | GGNCC | 2 cut(s) | 120, 180 |
| PspPPI | RGGWCCY | 1 cut(s) | 180 |
| Sau3AI | GATC | 1 cut(s) | 37 |
| Sau96I | GGNCC | 2 cut(s) | 120, 180 |
| SchI | GAGTC | 1 cut(s) | 103 |
| SduI | GDGCHC | 1 cut(s) | 8 |
| SetI | ASST | 2 cut(s) | 193, 223 |
| SfaNI | GCATC | 1 cut(s) | 94 |
| SinI | GGWCC | 1 cut(s) | 180 |
| Sse9I | AATT | 2 cut(s) | 230, 249 |
| SspMI | CTAG | 1 cut(s) | 150 |
| StyI | CCWWGG | 1 cut(s) | 169 |
| TaaI | ACNGT | 1 cut(s) | 46 |
| TaqI | TCGA | 2 cut(s) | 36, 68 |
| TasI | AATT | 2 cut(s) | 230, 249 |
| TscAI | CASTG | 1 cut(s) | 51 |
| TseFI | GTSAC | 2 cut(s) | 25, 79 |
| Tsp45I | GTSAC | 2 cut(s) | 25, 79 |
| TspRI | CASTG | 1 cut(s) | 51 |
| VpaK11BI | GGWCC | 1 cut(s) | 180 |
| XapI | RAATTY | 2 cut(s) | 230, 249 |
| XspI | CTAG | 1 cut(s) | 150 |