Plant transposase (Ptta/En/Spm family)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 2 cut(s) | 67, 114 |
| AccB1I | GGYRCC | 1 cut(s) | 48 |
| AccBSI | CCGCTC | 1 cut(s) | 87 |
| AccIII | TCCGGA | 1 cut(s) | 286 |
| AciI | CCGC | 2 cut(s) | 85, 146 |
| AcyI | GRCGYC | 2 cut(s) | 64, 111 |
| AfaI | GTAC | 1 cut(s) | 236 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 24 |
| AjnI | CCWGG | 1 cut(s) | 44 |
| AluBI | AGCT | 1 cut(s) | 274 |
| AluI | AGCT | 1 cut(s) | 274 |
| Alw21I | GWGCWC | 1 cut(s) | 96 |
| Alw26I | GTCTC | 2 cut(s) | 60, 212 |
| Alw44I | GTGCAC | 1 cut(s) | 92 |
| Ama87I | CYCGRG | 1 cut(s) | 194 |
| Aor13HI | TCCGGA | 1 cut(s) | 286 |
| AoxI | GGCC | 2 cut(s) | 40, 204 |
| ApaI | GGGCCC | 1 cut(s) | 44 |
| ApaLI | GTGCAC | 1 cut(s) | 92 |
| ApeKI | GCWGC | 1 cut(s) | 148 |
| AspLEI | GCGC | 1 cut(s) | 6 |
| AspS9I | GGNCC | 3 cut(s) | 40, 41, 126 |
| AsuHPI | GGTGA | 2 cut(s) | 73, 97 |
| AvaI | CYCGRG | 1 cut(s) | 194 |
| AvaII | GGWCC | 1 cut(s) | 126 |
| BaeGI | GKGCMC | 2 cut(s) | 44, 96 |
| BanI | GGYRCC | 1 cut(s) | 48 |
| BanII | GRGCYC | 1 cut(s) | 44 |
| Bbv12I | GWGCWC | 1 cut(s) | 96 |
| BbvI | GCAGC | 1 cut(s) | 160 |
| BciT130I | CCWGG | 1 cut(s) | 46 |
| BciVI | GTATCC | 2 cut(s) | 84, 274 |
| BclI | TGATCA | 1 cut(s) | 135 |
| BcoDI | GTCTC | 2 cut(s) | 60, 212 |
| BfuI | GTATCC | 2 cut(s) | 84, 274 |
| BisI | GCNGC | 1 cut(s) | 149 |
| BlsI | GCNGC | 1 cut(s) | 150 |
| Bme1390I | CCNGG | 1 cut(s) | 46 |
| Bme18I | GGWCC | 1 cut(s) | 126 |
| BmeT110I | CYCGRG | 1 cut(s) | 194 |
| BmgT120I | GGNCC | 3 cut(s) | 40, 41, 126 |
| BmiI | GGNNCC | 5 cut(s) | 41, 42, 43, 50, 128 |
| BmrFI | CCNGG | 1 cut(s) | 46 |
| BmsI | GCATC | 1 cut(s) | 160 |
| Bpu10I | CCTNAGC | 1 cut(s) | 223 |
| BsaHI | GRCGYC | 2 cut(s) | 64, 111 |
| BsaI | GGTCTC | 1 cut(s) | 212 |
| BsaJI | CCNNGG | 2 cut(s) | 44, 195 |
| BsaWI | WCCGGW | 2 cut(s) | 231, 286 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 24 |
| BseAI | TCCGGA | 1 cut(s) | 286 |
| BseBI | CCWGG | 1 cut(s) | 46 |
| BseDI | CCNNGG | 2 cut(s) | 44, 195 |
| BseGI | GGATG | 1 cut(s) | 151 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 24 |
| BseMII | CTCAG | 2 cut(s) | 14, 102 |
| BseSI | GKGCMC | 2 cut(s) | 44, 96 |
| BseXI | GCAGC | 1 cut(s) | 160 |
| BshFI | GGCC | 2 cut(s) | 42, 206 |
| BshNI | GGYRCC | 1 cut(s) | 48 |
| BsiHKAI | GWGCWC | 1 cut(s) | 96 |
| BsiHKCI | CYCGRG | 1 cut(s) | 194 |
| BsiSI | CCGG | 3 cut(s) | 124, 232, 287 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 24 |
| BsmAI | GTCTC | 2 cut(s) | 60, 212 |
| BsnI | GGCC | 2 cut(s) | 42, 206 |
| Bso31I | GGTCTC | 1 cut(s) | 212 |
| BsoBI | CYCGRG | 1 cut(s) | 194 |
| Bsp120I | GGGCCC | 1 cut(s) | 40 |
| Bsp1286I | GDGCHC | 2 cut(s) | 44, 96 |
| Bsp13I | TCCGGA | 1 cut(s) | 286 |
| Bsp143I | GATC | 2 cut(s) | 135, 175 |
| BspACI | CCGC | 2 cut(s) | 85, 146 |
| BspANI | GGCC | 2 cut(s) | 42, 206 |
| BspCNI | CTCAG | 2 cut(s) | 15, 101 |
| BspEI | TCCGGA | 1 cut(s) | 286 |
| BspLI | GGNNCC | 5 cut(s) | 41, 42, 43, 50, 128 |
| BspT107I | GGYRCC | 1 cut(s) | 48 |
| BspTNI | GGTCTC | 1 cut(s) | 212 |
| BsrBI | CCGCTC | 1 cut(s) | 87 |
| BssECI | CCNNGG | 2 cut(s) | 44, 195 |
| BssMI | GATC | 2 cut(s) | 135, 175 |
| BssNI | GRCGYC | 2 cut(s) | 64, 111 |
| Bst2UI | CCWGG | 1 cut(s) | 46 |
| BstACI | GRCGYC | 2 cut(s) | 64, 111 |
| BstC8I | GCNNGC | 2 cut(s) | 298, 336 |
| BstDEI | CTNAG | 4 cut(s) | 23, 88, 211, 223 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 22 |
| BstF5I | GGATG | 1 cut(s) | 151 |
| BstHHI | GCGC | 1 cut(s) | 6 |
| BstKTI | GATC | 2 cut(s) | 138, 178 |
| BstMAI | GTCTC | 2 cut(s) | 60, 212 |
| BstMBI | GATC | 2 cut(s) | 135, 175 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 48, 271 |
| BstNI | CCWGG | 1 cut(s) | 46 |
| BstSCI | CCNGG | 1 cut(s) | 44 |
| BstSLI | GKGCMC | 2 cut(s) | 44, 96 |
| BstV1I | GCAGC | 1 cut(s) | 160 |
| BsuI | GTATCC | 2 cut(s) | 84, 274 |
| BsuRI | GGCC | 2 cut(s) | 42, 206 |
| BtsCI | GGATG | 1 cut(s) | 151 |
| BtsIMutI | CAGTG | 1 cut(s) | 96 |
| Cac8I | GCNNGC | 2 cut(s) | 298, 336 |
| CfoI | GCGC | 1 cut(s) | 6 |
| Cfr13I | GGNCC | 3 cut(s) | 40, 41, 126 |
| Csp6I | GTAC | 1 cut(s) | 235 |
| CviAII | CATG | 1 cut(s) | 305 |
| CviJI | RGCY | 5 cut(s) | 28, 42, 123, 206, 274 |
| CviKI_1 | RGCY | 5 cut(s) | 28, 42, 123, 206, 274 |
| CviQI | GTAC | 1 cut(s) | 235 |
| DdeI | CTNAG | 4 cut(s) | 23, 88, 211, 223 |
| DpnI | GATC | 2 cut(s) | 137, 177 |
| DpnII | GATC | 2 cut(s) | 135, 175 |
| Eco147I | AGGCCT | 1 cut(s) | 206 |
| Eco24I | GRGCYC | 1 cut(s) | 44 |
| Eco31I | GGTCTC | 1 cut(s) | 212 |
| Eco47I | GGWCC | 1 cut(s) | 126 |
| Eco88I | CYCGRG | 1 cut(s) | 194 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 22 |
| EcoO109I | RGGNCCY | 2 cut(s) | 40, 41 |
| EcoRII | CCWGG | 1 cut(s) | 44 |
| EcoT38I | GRGCYC | 1 cut(s) | 44 |
| FaeI | CATG | 1 cut(s) | 308 |
| FaiI | YATR | 4 cut(s) | 37, 188, 277, 306 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 194, 226 |
| FatI | CATG | 1 cut(s) | 304 |
| FauI | CCCGC | 1 cut(s) | 92 |
| FbaI | TGATCA | 1 cut(s) | 135 |
| Fnu4HI | GCNGC | 1 cut(s) | 149 |
| FokI | GGATG | 1 cut(s) | 138 |
| FriOI | GRGCYC | 1 cut(s) | 44 |
| Fsp4HI | GCNGC | 1 cut(s) | 149 |
| GlaI | GCGC | 1 cut(s) | 5 |
| GluI | GCNGC | 1 cut(s) | 149 |
| HaeIII | GGCC | 2 cut(s) | 42, 206 |
| HapII | CCGG | 3 cut(s) | 124, 232, 287 |
| HhaI | GCGC | 1 cut(s) | 6 |
| Hin1I | GRCGYC | 2 cut(s) | 64, 111 |
| Hin1II | CATG | 1 cut(s) | 308 |
| Hin6I | GCGC | 1 cut(s) | 4 |
| HinP1I | GCGC | 1 cut(s) | 4 |
| HinfI | GANTC | 1 cut(s) | 158 |
| HpaII | CCGG | 3 cut(s) | 124, 232, 287 |
| HphI | GGTGA | 2 cut(s) | 73, 97 |
| Hpy166II | GTNNAC | 1 cut(s) | 94 |
| Hpy188I | TCNGA | 1 cut(s) | 135 |
| Hpy188III | TCNNGA | 5 cut(s) | 118, 155, 179, 194, 287 |
| Hpy8I | GTNNAC | 1 cut(s) | 94 |
| Hpy99I | CGWCG | 5 cut(s) | 66, 101, 113, 116, 119 |
| HpyAV | CCTTC | 1 cut(s) | 140 |
| HpyCH4IV | ACGT | 2 cut(s) | 64, 111 |
| HpyCH4V | TGCA | 1 cut(s) | 94 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 48, 271 |
| HpyF3I | CTNAG | 4 cut(s) | 23, 88, 211, 223 |
| HpySE526I | ACGT | 2 cut(s) | 64, 111 |
| Hsp92I | GRCGYC | 2 cut(s) | 64, 111 |
| Hsp92II | CATG | 1 cut(s) | 308 |
| HspAI | GCGC | 1 cut(s) | 4 |
| Kpn2I | TCCGGA | 1 cut(s) | 286 |
| Ksp22I | TGATCA | 1 cut(s) | 135 |
| Kzo9I | GATC | 2 cut(s) | 135, 175 |
| LpnPI | CCDG | 8 cut(s) | 31, 58, 65, 137, 168, 245, 300, 320 |
| Lsp1109I | GCAGC | 1 cut(s) | 160 |
| LweI | GCATC | 1 cut(s) | 160 |
| MaeII | ACGT | 2 cut(s) | 64, 111 |
| MalI | GATC | 2 cut(s) | 137, 177 |
| MbiI | CCGCTC | 1 cut(s) | 87 |
| MboI | GATC | 2 cut(s) | 135, 175 |
| MboII | GAAGA | 2 cut(s) | 248, 259 |
| MhlI | GDGCHC | 2 cut(s) | 44, 96 |
| MluCI | AATT | 1 cut(s) | 168 |
| MlyI | GAGTC | 1 cut(s) | 167 |
| MnlI | CCTC | 5 cut(s) | 17, 18, 113, 156, 190 |
| MroI | TCCGGA | 1 cut(s) | 286 |
| MseI | TTAA | 1 cut(s) | 270 |
| MspI | CCGG | 3 cut(s) | 124, 232, 287 |
| MspR9I | CCNGG | 1 cut(s) | 46 |
| MvaI | CCWGG | 1 cut(s) | 46 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 48, 271 |
| NdeII | GATC | 2 cut(s) | 135, 175 |
| NlaIII | CATG | 1 cut(s) | 308 |
| NlaIV | GGNNCC | 5 cut(s) | 41, 42, 43, 50, 128 |
| PceI | AGGCCT | 1 cut(s) | 206 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 105 |
| PflFI | GACNNNGTC | 1 cut(s) | 113 |
| PkrI | GCNGC | 1 cut(s) | 150 |
| PleI | GAGTC | 1 cut(s) | 166 |
| PpsI | GAGTC | 1 cut(s) | 166 |
| Psp6I | CCWGG | 1 cut(s) | 44 |
| PspGI | CCWGG | 1 cut(s) | 44 |
| PspN4I | GGNNCC | 5 cut(s) | 41, 42, 43, 50, 128 |
| PspOMI | GGGCCC | 1 cut(s) | 40 |
| PspPI | GGNCC | 3 cut(s) | 40, 41, 126 |
| PsyI | GACNNNGTC | 1 cut(s) | 113 |
| RsaI | GTAC | 1 cut(s) | 236 |
| RsaNI | GTAC | 1 cut(s) | 235 |
| SaqAI | TTAA | 1 cut(s) | 270 |
| SatI | GCNGC | 1 cut(s) | 149 |
| Sau3AI | GATC | 2 cut(s) | 135, 175 |
| Sau96I | GGNCC | 3 cut(s) | 40, 41, 126 |
| SchI | GAGTC | 1 cut(s) | 167 |
| ScrFI | CCNGG | 1 cut(s) | 46 |
| SduI | GDGCHC | 2 cut(s) | 44, 96 |
| SetI | ASST | 7 cut(s) | 50, 67, 105, 114, 201, 224, 276 |
| SfaNI | GCATC | 1 cut(s) | 160 |
| SinI | GGWCC | 1 cut(s) | 126 |
| Sse9I | AATT | 1 cut(s) | 168 |
| SseBI | AGGCCT | 1 cut(s) | 206 |
| SsiI | CCGC | 2 cut(s) | 85, 146 |
| StuI | AGGCCT | 1 cut(s) | 206 |
| StyD4I | CCNGG | 1 cut(s) | 44 |
| TaiI | ACGT | 2 cut(s) | 67, 114 |
| TaqI | TCGA | 3 cut(s) | 61, 161, 174 |
| TasI | AATT | 1 cut(s) | 168 |
| Tru1I | TTAA | 1 cut(s) | 270 |
| Tru9I | TTAA | 1 cut(s) | 270 |
| TscAI | CASTG | 1 cut(s) | 96 |
| TseI | GCWGC | 1 cut(s) | 148 |
| TspRI | CASTG | 1 cut(s) | 96 |
| Tth111I | GACNNNGTC | 1 cut(s) | 113 |
| VneI | GTGCAC | 1 cut(s) | 92 |
| VpaK11BI | GGWCC | 1 cut(s) | 126 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 22 |
| ZraI | GACGTC | 2 cut(s) | 65, 112 |