Plant transposase (Ptta/En/Spm family)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 595 |
| AccB1I | GGYRCC | 1 cut(s) | 595 |
| AccI | GTMKAC | 1 cut(s) | 10 |
| AciI | CCGC | 3 cut(s) | 214, 299, 415 |
| AclWI | GGATC | 1 cut(s) | 609 |
| AcvI | CACGTG | 1 cut(s) | 449 |
| AfaI | GTAC | 3 cut(s) | 246, 597, 659 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 628 |
| AflIII | ACRYGT | 1 cut(s) | 446 |
| AgsI | TTSAA | 1 cut(s) | 434 |
| AluBI | AGCT | 2 cut(s) | 169, 526 |
| AluI | AGCT | 2 cut(s) | 169, 526 |
| Alw26I | GTCTC | 2 cut(s) | 42, 179 |
| AlwI | GGATC | 1 cut(s) | 609 |
| AoxI | GGCC | 2 cut(s) | 177, 639 |
| ApeKI | GCWGC | 3 cut(s) | 283, 380, 523 |
| AseI | ATTAAT | 1 cut(s) | 426 |
| Asp718I | GGTACC | 1 cut(s) | 595 |
| AspLEI | GCGC | 3 cut(s) | 87, 202, 606 |
| AspS9I | GGNCC | 1 cut(s) | 653 |
| AsuC2I | CCSGG | 1 cut(s) | 204 |
| AsuHPI | GGTGA | 1 cut(s) | 194 |
| AvaII | GGWCC | 1 cut(s) | 653 |
| BanI | GGYRCC | 1 cut(s) | 595 |
| BbrPI | CACGTG | 1 cut(s) | 449 |
| BbsI | GAAGAC | 1 cut(s) | 468 |
| BbvCI | CCTCAGC | 1 cut(s) | 510 |
| BbvI | GCAGC | 3 cut(s) | 295, 392, 535 |
| BccI | CCATC | 3 cut(s) | 571, 626, 650 |
| BcnI | CCSGG | 1 cut(s) | 204 |
| BcoDI | GTCTC | 2 cut(s) | 42, 179 |
| BfaI | CTAG | 1 cut(s) | 661 |
| BfmI | CTRYAG | 3 cut(s) | 468, 531, 579 |
| BisI | GCNGC | 3 cut(s) | 284, 381, 524 |
| BlsI | GCNGC | 3 cut(s) | 285, 382, 525 |
| Bme1390I | CCNGG | 1 cut(s) | 204 |
| Bme18I | GGWCC | 1 cut(s) | 653 |
| BmgT120I | GGNCC | 1 cut(s) | 653 |
| BmiI | GGNNCC | 1 cut(s) | 597 |
| BmrFI | CCNGG | 1 cut(s) | 204 |
| BmsI | GCATC | 2 cut(s) | 216, 565 |
| BpiI | GAAGAC | 1 cut(s) | 468 |
| Bpu10I | CCTNAGC | 1 cut(s) | 510 |
| BpuMI | CCSGG | 1 cut(s) | 204 |
| BsaAI | YACGTR | 1 cut(s) | 449 |
| BsaJI | CCNNGG | 4 cut(s) | 115, 203, 348, 492 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 628 |
| Bse118I | RCCGGY | 2 cut(s) | 179, 628 |
| Bse1I | ACTGG | 1 cut(s) | 655 |
| BseDI | CCNNGG | 4 cut(s) | 115, 203, 348, 492 |
| BseGI | GGATG | 1 cut(s) | 618 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 628 |
| BseMII | CTCAG | 2 cut(s) | 501, 537 |
| BseNI | ACTGG | 1 cut(s) | 655 |
| BseRI | GAGGAG | 2 cut(s) | 329, 338 |
| BseXI | GCAGC | 3 cut(s) | 295, 392, 535 |
| BshFI | GGCC | 2 cut(s) | 179, 641 |
| BshNI | GGYRCC | 1 cut(s) | 595 |
| BsiSI | CCGG | 3 cut(s) | 180, 203, 629 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 628 |
| BsmAI | GTCTC | 2 cut(s) | 42, 179 |
| BsmI | GAATGC | 2 cut(s) | 298, 478 |
| BsnI | GGCC | 2 cut(s) | 179, 641 |
| Bsp143I | GATC | 2 cut(s) | 39, 614 |
| BspACI | CCGC | 3 cut(s) | 214, 299, 415 |
| BspANI | GGCC | 2 cut(s) | 179, 641 |
| BspCNI | CTCAG | 2 cut(s) | 502, 538 |
| BspLI | GGNNCC | 1 cut(s) | 597 |
| BspPI | GGATC | 1 cut(s) | 609 |
| BspT107I | GGYRCC | 1 cut(s) | 595 |
| BsrFI | RCCGGY | 2 cut(s) | 179, 628 |
| BsrI | ACTGG | 1 cut(s) | 655 |
| BssAI | RCCGGY | 2 cut(s) | 179, 628 |
| BssECI | CCNNGG | 4 cut(s) | 115, 203, 348, 492 |
| BssMI | GATC | 2 cut(s) | 39, 614 |
| BssNAI | GTATAC | 1 cut(s) | 11 |
| BssT1I | CCWWGG | 2 cut(s) | 115, 492 |
| Bst1107I | GTATAC | 1 cut(s) | 11 |
| Bst4CI | ACNGT | 5 cut(s) | 190, 343, 487, 580, 657 |
| Bst6I | CTCTTC | 1 cut(s) | 306 |
| BstBAI | YACGTR | 1 cut(s) | 449 |
| BstC8I | GCNNGC | 3 cut(s) | 112, 240, 630 |
| BstDEI | CTNAG | 3 cut(s) | 510, 538, 546 |
| BstF5I | GGATG | 1 cut(s) | 618 |
| BstHHI | GCGC | 3 cut(s) | 87, 202, 606 |
| BstKTI | GATC | 2 cut(s) | 42, 617 |
| BstMAI | GTCTC | 2 cut(s) | 42, 179 |
| BstMBI | GATC | 2 cut(s) | 39, 614 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 235, 515, 638 |
| BstSCI | CCNGG | 1 cut(s) | 202 |
| BstSFI | CTRYAG | 3 cut(s) | 468, 531, 579 |
| BstV1I | GCAGC | 3 cut(s) | 295, 392, 535 |
| BstV2I | GAAGAC | 1 cut(s) | 468 |
| BstX2I | RGATCY | 1 cut(s) | 614 |
| BstYI | RGATCY | 1 cut(s) | 614 |
| BstZ17I | GTATAC | 1 cut(s) | 11 |
| BsuRI | GGCC | 2 cut(s) | 179, 641 |
| BtsCI | GGATG | 1 cut(s) | 618 |
| BtsI | GCAGTG | 1 cut(s) | 599 |
| BtsIMutI | CAGTG | 1 cut(s) | 599 |
| Cac8I | GCNNGC | 3 cut(s) | 112, 240, 630 |
| CfoI | GCGC | 3 cut(s) | 87, 202, 606 |
| Cfr10I | RCCGGY | 2 cut(s) | 179, 628 |
| Cfr13I | GGNCC | 1 cut(s) | 653 |
| CseI | GACGC | 1 cut(s) | 377 |
| Csp6I | GTAC | 3 cut(s) | 245, 596, 658 |
| CviAII | CATG | 1 cut(s) | 88 |
| CviJI | RGCY | 7 cut(s) | 5, 169, 179, 509, 526, 537, 641 |
| CviKI_1 | RGCY | 7 cut(s) | 5, 169, 179, 509, 526, 537, 641 |
| CviQI | GTAC | 3 cut(s) | 245, 596, 658 |
| DdeI | CTNAG | 3 cut(s) | 510, 538, 546 |
| DpnI | GATC | 2 cut(s) | 41, 616 |
| DpnII | GATC | 2 cut(s) | 39, 614 |
| Eam1104I | CTCTTC | 1 cut(s) | 306 |
| EarI | CTCTTC | 1 cut(s) | 306 |
| Eco130I | CCWWGG | 2 cut(s) | 115, 492 |
| Eco47I | GGWCC | 1 cut(s) | 653 |
| Eco72I | CACGTG | 1 cut(s) | 449 |
| EcoT14I | CCWWGG | 2 cut(s) | 115, 492 |
| ErhI | CCWWGG | 2 cut(s) | 115, 492 |
| FaeI | CATG | 1 cut(s) | 91 |
| FaiI | YATR | 9 cut(s) | 11, 89, 129, 264, 275, 372, 470, 533, 591 |
| FatI | CATG | 1 cut(s) | 87 |
| FblI | GTMKAC | 1 cut(s) | 10 |
| Fnu4HI | GCNGC | 3 cut(s) | 284, 381, 524 |
| FokI | GGATG | 1 cut(s) | 605 |
| Fsp4HI | GCNGC | 3 cut(s) | 284, 381, 524 |
| FspBI | CTAG | 1 cut(s) | 661 |
| GlaI | GCGC | 3 cut(s) | 86, 201, 605 |
| GluI | GCNGC | 3 cut(s) | 284, 381, 524 |
| HaeIII | GGCC | 2 cut(s) | 179, 641 |
| HapII | CCGG | 3 cut(s) | 180, 203, 629 |
| HgaI | GACGC | 1 cut(s) | 377 |
| HhaI | GCGC | 3 cut(s) | 87, 202, 606 |
| Hin1II | CATG | 1 cut(s) | 91 |
| Hin6I | GCGC | 3 cut(s) | 85, 200, 604 |
| HinP1I | GCGC | 3 cut(s) | 85, 200, 604 |
| HincII | GTYRAC | 1 cut(s) | 576 |
| HindII | GTYRAC | 1 cut(s) | 576 |
| HinfI | GANTC | 2 cut(s) | 77, 94 |
| HpaII | CCGG | 3 cut(s) | 180, 203, 629 |
| HphI | GGTGA | 1 cut(s) | 194 |
| Hpy166II | GTNNAC | 2 cut(s) | 11, 576 |
| Hpy188I | TCNGA | 6 cut(s) | 39, 47, 76, 82, 349, 612 |
| Hpy188III | TCNNGA | 3 cut(s) | 392, 431, 442 |
| Hpy8I | GTNNAC | 2 cut(s) | 11, 576 |
| Hpy99I | CGWCG | 2 cut(s) | 65, 157 |
| HpyAV | CCTTC | 5 cut(s) | 41, 187, 313, 617, 631 |
| HpyCH4III | ACNGT | 5 cut(s) | 190, 343, 487, 580, 657 |
| HpyCH4IV | ACGT | 3 cut(s) | 60, 448, 552 |
| HpyCH4V | TGCA | 3 cut(s) | 452, 478, 556 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 235, 515, 638 |
| HpyF3I | CTNAG | 3 cut(s) | 510, 538, 546 |
| HpySE526I | ACGT | 3 cut(s) | 60, 448, 552 |
| Hsp92II | CATG | 1 cut(s) | 91 |
| HspAI | GCGC | 3 cut(s) | 85, 200, 604 |
| KpnI | GGTACC | 1 cut(s) | 599 |
| KroI | GCCGGC | 1 cut(s) | 628 |
| KroNI | GCCGGC | 1 cut(s) | 630 |
| Kzo9I | GATC | 2 cut(s) | 39, 614 |
| LmnI | GCTCC | 2 cut(s) | 82, 301 |
| Lsp1109I | GCAGC | 3 cut(s) | 295, 392, 535 |
| LweI | GCATC | 2 cut(s) | 216, 565 |
| MaeI | CTAG | 1 cut(s) | 661 |
| MaeII | ACGT | 3 cut(s) | 60, 448, 552 |
| MaeIII | GTNAC | 2 cut(s) | 337, 487 |
| MalI | GATC | 2 cut(s) | 41, 616 |
| MboI | GATC | 2 cut(s) | 39, 614 |
| MboII | GAAGA | 5 cut(s) | 63, 317, 320, 323, 473 |
| MflI | RGATCY | 1 cut(s) | 614 |
| MluCI | AATT | 3 cut(s) | 134, 233, 473 |
| MlyI | GAGTC | 1 cut(s) | 86 |
| MmeI | TCCRAC | 1 cut(s) | 60 |
| MnlI | CCTC | 9 cut(s) | 108, 307, 316, 319, 343, 411, 490, 505, 586 |
| MroNI | GCCGGC | 1 cut(s) | 628 |
| MseI | TTAA | 3 cut(s) | 330, 357, 426 |
| MspA1I | CMGCKG | 1 cut(s) | 301 |
| MspI | CCGG | 3 cut(s) | 180, 203, 629 |
| MspR9I | CCNGG | 1 cut(s) | 204 |
| Mva1269I | GAATGC | 2 cut(s) | 298, 478 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 235, 515, 638 |
| NaeI | GCCGGC | 1 cut(s) | 630 |
| NciI | CCSGG | 1 cut(s) | 204 |
| NdeII | GATC | 2 cut(s) | 39, 614 |
| NgoMIV | GCCGGC | 1 cut(s) | 628 |
| NlaIII | CATG | 1 cut(s) | 91 |
| NlaIV | GGNNCC | 1 cut(s) | 597 |
| NmeAIII | GCCGAG | 1 cut(s) | 150 |
| NmuCI | GTSAC | 1 cut(s) | 487 |
| PctI | GAATGC | 2 cut(s) | 298, 478 |
| PdiI | GCCGGC | 1 cut(s) | 630 |
| PfeI | GAWTC | 1 cut(s) | 94 |
| PkrI | GCNGC | 3 cut(s) | 285, 382, 525 |
| PleI | GAGTC | 1 cut(s) | 85 |
| PmaCI | CACGTG | 1 cut(s) | 449 |
| PmlI | CACGTG | 1 cut(s) | 449 |
| PpsI | GAGTC | 1 cut(s) | 85 |
| Ppu21I | YACGTR | 1 cut(s) | 449 |
| PshBI | ATTAAT | 1 cut(s) | 426 |
| PspCI | CACGTG | 1 cut(s) | 449 |
| PspN4I | GGNNCC | 1 cut(s) | 597 |
| PspPI | GGNCC | 1 cut(s) | 653 |
| PsuI | RGATCY | 1 cut(s) | 614 |
| RsaI | GTAC | 3 cut(s) | 246, 597, 659 |
| RsaNI | GTAC | 3 cut(s) | 245, 596, 658 |
| SaqAI | TTAA | 3 cut(s) | 330, 357, 426 |
| SatI | GCNGC | 3 cut(s) | 284, 381, 524 |
| Sau3AI | GATC | 2 cut(s) | 39, 614 |
| Sau96I | GGNCC | 1 cut(s) | 653 |
| SchI | GAGTC | 1 cut(s) | 86 |
| ScrFI | CCNGG | 1 cut(s) | 204 |
| SfaNI | GCATC | 2 cut(s) | 216, 565 |
| SfcI | CTRYAG | 3 cut(s) | 468, 531, 579 |
| SinI | GGWCC | 1 cut(s) | 653 |
| Sse9I | AATT | 3 cut(s) | 134, 233, 473 |
| SsiI | CCGC | 3 cut(s) | 214, 299, 415 |
| SspMI | CTAG | 1 cut(s) | 661 |
| StyD4I | CCNGG | 1 cut(s) | 202 |
| StyI | CCWWGG | 2 cut(s) | 115, 492 |
| TaaI | ACNGT | 5 cut(s) | 190, 343, 487, 580, 657 |
| TaiI | ACGT | 3 cut(s) | 63, 451, 555 |
| TasI | AATT | 3 cut(s) | 134, 233, 473 |
| TfiI | GAWTC | 1 cut(s) | 94 |
| Tru1I | TTAA | 3 cut(s) | 330, 357, 426 |
| Tru9I | TTAA | 3 cut(s) | 330, 357, 426 |
| TscAI | CASTG | 1 cut(s) | 606 |
| TseFI | GTSAC | 1 cut(s) | 487 |
| TseI | GCWGC | 3 cut(s) | 283, 380, 523 |
| Tsp45I | GTSAC | 1 cut(s) | 487 |
| TspDTI | ATGAA | 3 cut(s) | 107, 377, 387 |
| TspRI | CASTG | 1 cut(s) | 606 |
| VpaK11BI | GGWCC | 1 cut(s) | 653 |
| VspI | ATTAAT | 1 cut(s) | 426 |
| XmiI | GTMKAC | 1 cut(s) | 10 |
| XspI | CTAG | 1 cut(s) | 661 |