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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 2 cut(s) | 310, 474 |
| Acc36I | ACCTGC | 1 cut(s) | 482 |
| AciI | CCGC | 2 cut(s) | 116, 574 |
| AclWI | GGATC | 1 cut(s) | 528 |
| AcoI | YGGCCR | 1 cut(s) | 161 |
| AcsI | RAATTY | 2 cut(s) | 206, 218 |
| AcuI | CTGAAG | 1 cut(s) | 482 |
| AcyI | GRCGYC | 2 cut(s) | 307, 471 |
| AfaI | GTAC | 2 cut(s) | 65, 480 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 166, 511 |
| AgsI | TTSAA | 2 cut(s) | 11, 276 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 512, 544 |
| AluBI | AGCT | 4 cut(s) | 103, 239, 388, 445 |
| AluI | AGCT | 4 cut(s) | 103, 239, 388, 445 |
| Alw26I | GTCTC | 2 cut(s) | 368, 578 |
| AlwI | GGATC | 1 cut(s) | 528 |
| Ama87I | CYCGRG | 1 cut(s) | 240 |
| AoxI | GGCC | 3 cut(s) | 27, 33, 161 |
| ApeKI | GCWGC | 3 cut(s) | 385, 395, 442 |
| ApoI | RAATTY | 2 cut(s) | 206, 218 |
| AspS9I | GGNCC | 1 cut(s) | 157 |
| AsuC2I | CCSGG | 1 cut(s) | 61 |
| AvaI | CYCGRG | 1 cut(s) | 240 |
| AvaII | GGWCC | 1 cut(s) | 157 |
| AxyI | CCTNAGG | 1 cut(s) | 483 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 240, 272 |
| BbsI | GAAGAC | 1 cut(s) | 488 |
| BbvI | GCAGC | 3 cut(s) | 382, 397, 454 |
| BcnI | CCSGG | 1 cut(s) | 61 |
| BcoDI | GTCTC | 2 cut(s) | 368, 578 |
| BfaI | CTAG | 2 cut(s) | 131, 296 |
| BfuAI | ACCTGC | 1 cut(s) | 482 |
| BglII | AGATCT | 1 cut(s) | 456 |
| BisI | GCNGC | 3 cut(s) | 386, 396, 443 |
| BlsI | GCNGC | 3 cut(s) | 387, 397, 444 |
| Bme1390I | CCNGG | 1 cut(s) | 61 |
| Bme18I | GGWCC | 1 cut(s) | 157 |
| BmeT110I | CYCGRG | 1 cut(s) | 240 |
| BmgT120I | GGNCC | 1 cut(s) | 157 |
| BmrFI | CCNGG | 1 cut(s) | 61 |
| BmsI | GCATC | 3 cut(s) | 103, 478, 580 |
| BpiI | GAAGAC | 1 cut(s) | 488 |
| Bpu10I | CCTNAGC | 1 cut(s) | 52 |
| BpuMI | CCSGG | 1 cut(s) | 61 |
| BsaBI | GATNNNNATC | 1 cut(s) | 461 |
| BsaHI | GRCGYC | 2 cut(s) | 307, 471 |
| BsaI | GGTCTC | 1 cut(s) | 368 |
| BsaJI | CCNNGG | 3 cut(s) | 24, 30, 450 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 166, 511 |
| Bse118I | RCCGGY | 1 cut(s) | 159 |
| Bse21I | CCTNAGG | 1 cut(s) | 483 |
| Bse8I | GATNNNNATC | 1 cut(s) | 461 |
| BseDI | CCNNGG | 3 cut(s) | 24, 30, 450 |
| BseGI | GGATG | 3 cut(s) | 115, 493, 595 |
| BseJI | GATNNNNATC | 1 cut(s) | 461 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 166, 511 |
| BseMII | CTCAG | 3 cut(s) | 372, 474, 501 |
| BseXI | GCAGC | 3 cut(s) | 382, 397, 454 |
| BseYI | CCCAGC | 1 cut(s) | 142 |
| Bsh1285I | CGRYCG | 1 cut(s) | 465 |
| BshFI | GGCC | 3 cut(s) | 29, 35, 163 |
| BsiEI | CGRYCG | 1 cut(s) | 465 |
| BsiHKCI | CYCGRG | 1 cut(s) | 240 |
| BsiSI | CCGG | 2 cut(s) | 61, 160 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 166, 511 |
| BsmAI | GTCTC | 2 cut(s) | 368, 578 |
| BsmBI | CGTCTC | 1 cut(s) | 578 |
| BsnI | GGCC | 3 cut(s) | 29, 35, 163 |
| Bso31I | GGTCTC | 1 cut(s) | 368 |
| BsoBI | CYCGRG | 1 cut(s) | 240 |
| Bsp143I | GATC | 8 cut(s) | 3, 82, 333, 456, 462, 520, 532, 605 |
| Bsp19I | CCATGG | 1 cut(s) | 30 |
| BspACI | CCGC | 2 cut(s) | 116, 574 |
| BspANI | GGCC | 3 cut(s) | 29, 35, 163 |
| BspCNI | CTCAG | 3 cut(s) | 373, 475, 502 |
| BspMI | ACCTGC | 1 cut(s) | 482 |
| BspPI | GGATC | 1 cut(s) | 528 |
| BspQI | GCTCTTC | 1 cut(s) | 71 |
| BspTNI | GGTCTC | 1 cut(s) | 368 |
| BsrFI | RCCGGY | 1 cut(s) | 159 |
| BssAI | RCCGGY | 1 cut(s) | 159 |
| BssECI | CCNNGG | 3 cut(s) | 24, 30, 450 |
| BssMI | GATC | 8 cut(s) | 3, 82, 333, 456, 462, 520, 532, 605 |
| BssNI | GRCGYC | 2 cut(s) | 307, 471 |
| BssT1I | CCWWGG | 2 cut(s) | 24, 30 |
| Bst6I | CTCTTC | 1 cut(s) | 71 |
| BstACI | GRCGYC | 2 cut(s) | 307, 471 |
| BstC8I | GCNNGC | 1 cut(s) | 229 |
| BstDEI | CTNAG | 6 cut(s) | 40, 52, 321, 381, 483, 510 |
| BstDSI | CCRYGG | 1 cut(s) | 30 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 509 |
| BstF5I | GGATG | 3 cut(s) | 115, 493, 595 |
| BstKTI | GATC | 8 cut(s) | 6, 85, 336, 459, 465, 523, 535, 608 |
| BstMAI | GTCTC | 2 cut(s) | 368, 578 |
| BstMBI | GATC | 8 cut(s) | 3, 82, 333, 456, 462, 520, 532, 605 |
| BstMCI | CGRYCG | 1 cut(s) | 465 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 100, 122 |
| BstSCI | CCNGG | 1 cut(s) | 59 |
| BstV1I | GCAGC | 3 cut(s) | 382, 397, 454 |
| BstV2I | GAAGAC | 1 cut(s) | 488 |
| BstX2I | RGATCY | 1 cut(s) | 456 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 31, 171 |
| BstYI | RGATCY | 1 cut(s) | 456 |
| Bsu36I | CCTNAGG | 1 cut(s) | 483 |
| BsuRI | GGCC | 3 cut(s) | 29, 35, 163 |
| BtgI | CCRYGG | 1 cut(s) | 30 |
| BtgZI | GCGATG | 1 cut(s) | 423 |
| BtsCI | GGATG | 3 cut(s) | 115, 493, 595 |
| BtsI | GCAGTG | 1 cut(s) | 414 |
| BtsIMutI | CAGTG | 2 cut(s) | 378, 414 |
| BveI | ACCTGC | 1 cut(s) | 482 |
| Cac8I | GCNNGC | 1 cut(s) | 229 |
| Cfr10I | RCCGGY | 1 cut(s) | 159 |
| Cfr13I | GGNCC | 1 cut(s) | 157 |
| Csp6I | GTAC | 2 cut(s) | 64, 479 |
| CviAII | CATG | 5 cut(s) | 31, 89, 392, 542, 615 |
| CviQI | GTAC | 2 cut(s) | 64, 479 |
| DdeI | CTNAG | 6 cut(s) | 40, 52, 321, 381, 483, 510 |
| DpnI | GATC | 8 cut(s) | 5, 84, 335, 458, 464, 522, 534, 607 |
| DpnII | GATC | 8 cut(s) | 3, 82, 333, 456, 462, 520, 532, 605 |
| EaeI | YGGCCR | 1 cut(s) | 161 |
| Eam1104I | CTCTTC | 1 cut(s) | 71 |
| EarI | CTCTTC | 1 cut(s) | 71 |
| Eco130I | CCWWGG | 2 cut(s) | 24, 30 |
| Eco31I | GGTCTC | 1 cut(s) | 368 |
| Eco47I | GGWCC | 1 cut(s) | 157 |
| Eco57I | CTGAAG | 1 cut(s) | 482 |
| Eco81I | CCTNAGG | 1 cut(s) | 483 |
| Eco88I | CYCGRG | 1 cut(s) | 240 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 509 |
| EcoT14I | CCWWGG | 2 cut(s) | 24, 30 |
| ErhI | CCWWGG | 2 cut(s) | 24, 30 |
| Esp3I | CGTCTC | 1 cut(s) | 578 |
| FaeI | CATG | 5 cut(s) | 34, 92, 395, 545, 618 |
| FatI | CATG | 5 cut(s) | 30, 88, 391, 541, 614 |
| FauI | CCCGC | 1 cut(s) | 567 |
| Fnu4HI | GCNGC | 3 cut(s) | 386, 396, 443 |
| FokI | GGATG | 3 cut(s) | 122, 500, 602 |
| Fsp4HI | GCNGC | 3 cut(s) | 386, 396, 443 |
| FspBI | CTAG | 2 cut(s) | 131, 296 |
| GluI | GCNGC | 3 cut(s) | 386, 396, 443 |
| GsaI | CCCAGC | 1 cut(s) | 146 |
| HaeIII | GGCC | 3 cut(s) | 29, 35, 163 |
| HapII | CCGG | 2 cut(s) | 61, 160 |
| Hin1I | GRCGYC | 2 cut(s) | 307, 471 |
| Hin1II | CATG | 5 cut(s) | 34, 92, 395, 545, 618 |
| HinfI | GANTC | 2 cut(s) | 200, 363 |
| HpaII | CCGG | 2 cut(s) | 61, 160 |
| Hpy166II | GTNNAC | 1 cut(s) | 355 |
| Hpy188I | TCNGA | 3 cut(s) | 454, 501, 525 |
| Hpy188III | TCNNGA | 8 cut(s) | 11, 131, 242, 311, 460, 518, 581, 603 |
| Hpy8I | GTNNAC | 1 cut(s) | 355 |
| Hpy99I | CGWCG | 1 cut(s) | 309 |
| HpyAV | CCTTC | 1 cut(s) | 562 |
| HpyCH4IV | ACGT | 3 cut(s) | 307, 351, 471 |
| HpyCH4V | TGCA | 3 cut(s) | 94, 186, 491 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 100, 122 |
| HpyF3I | CTNAG | 6 cut(s) | 40, 52, 321, 381, 483, 510 |
| HpySE526I | ACGT | 3 cut(s) | 307, 351, 471 |
| Hsp92I | GRCGYC | 2 cut(s) | 307, 471 |
| Hsp92II | CATG | 5 cut(s) | 34, 92, 395, 545, 618 |
| Kzo9I | GATC | 8 cut(s) | 3, 82, 333, 456, 462, 520, 532, 605 |
| LguI | GCTCTTC | 1 cut(s) | 71 |
| Lsp1109I | GCAGC | 3 cut(s) | 382, 397, 454 |
| LweI | GCATC | 3 cut(s) | 103, 478, 580 |
| MaeI | CTAG | 2 cut(s) | 131, 296 |
| MaeII | ACGT | 3 cut(s) | 307, 351, 471 |
| MalI | GATC | 8 cut(s) | 5, 84, 335, 458, 464, 522, 534, 607 |
| MboI | GATC | 8 cut(s) | 3, 82, 333, 456, 462, 520, 532, 605 |
| MboII | GAAGA | 4 cut(s) | 77, 88, 226, 488 |
| MflI | RGATCY | 1 cut(s) | 456 |
| MluCI | AATT | 3 cut(s) | 70, 206, 218 |
| MlyI | GAGTC | 2 cut(s) | 194, 357 |
| MmeI | TCCRAC | 1 cut(s) | 132 |
| MseI | TTAA | 2 cut(s) | 99, 345 |
| MslI | CAYNNNNRTG | 1 cut(s) | 540 |
| MspI | CCGG | 2 cut(s) | 61, 160 |
| MspR9I | CCNGG | 1 cut(s) | 61 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 100, 122 |
| NciI | CCSGG | 1 cut(s) | 61 |
| NcoI | CCATGG | 1 cut(s) | 30 |
| NdeII | GATC | 8 cut(s) | 3, 82, 333, 456, 462, 520, 532, 605 |
| NlaIII | CATG | 5 cut(s) | 34, 92, 395, 545, 618 |
| NmeAIII | GCCGAG | 1 cut(s) | 172 |
| PciSI | GCTCTTC | 1 cut(s) | 71 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 458 |
| PkrI | GCNGC | 3 cut(s) | 387, 397, 444 |
| Ple19I | CGATCG | 1 cut(s) | 465 |
| PleI | GAGTC | 2 cut(s) | 194, 357 |
| PpsI | GAGTC | 2 cut(s) | 194, 357 |
| PspFI | CCCAGC | 1 cut(s) | 142 |
| PspPI | GGNCC | 1 cut(s) | 157 |
| PsuI | RGATCY | 1 cut(s) | 456 |
| PvuI | CGATCG | 1 cut(s) | 465 |
| RsaI | GTAC | 2 cut(s) | 65, 480 |
| RsaNI | GTAC | 2 cut(s) | 64, 479 |
| RseI | CAYNNNNRTG | 1 cut(s) | 540 |
| SapI | GCTCTTC | 1 cut(s) | 71 |
| SaqAI | TTAA | 2 cut(s) | 99, 345 |
| SatI | GCNGC | 3 cut(s) | 386, 396, 443 |
| Sau3AI | GATC | 8 cut(s) | 3, 82, 333, 456, 462, 520, 532, 605 |
| Sau96I | GGNCC | 1 cut(s) | 157 |
| SchI | GAGTC | 2 cut(s) | 194, 357 |
| ScrFI | CCNGG | 1 cut(s) | 61 |
| SfaNI | GCATC | 3 cut(s) | 103, 478, 580 |
| SinI | GGWCC | 1 cut(s) | 157 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 540 |
| Sse9I | AATT | 3 cut(s) | 70, 206, 218 |
| SsiI | CCGC | 2 cut(s) | 116, 574 |
| SspMI | CTAG | 2 cut(s) | 131, 296 |
| StyD4I | CCNGG | 1 cut(s) | 59 |
| StyI | CCWWGG | 2 cut(s) | 24, 30 |
| TaiI | ACGT | 3 cut(s) | 310, 354, 474 |
| TaqI | TCGA | 7 cut(s) | 336, 361, 366, 461, 465, 531, 608 |
| TasI | AATT | 3 cut(s) | 70, 206, 218 |
| Tru1I | TTAA | 2 cut(s) | 99, 345 |
| Tru9I | TTAA | 2 cut(s) | 99, 345 |
| TscAI | CASTG | 2 cut(s) | 385, 421 |
| TseI | GCWGC | 3 cut(s) | 385, 395, 442 |
| TspDTI | ATGAA | 3 cut(s) | 77, 227, 380 |
| TspGWI | ACGGA | 2 cut(s) | 212, 602 |
| TspRI | CASTG | 2 cut(s) | 385, 421 |
| VpaK11BI | GGWCC | 1 cut(s) | 157 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 509 |
| XapI | RAATTY | 2 cut(s) | 206, 218 |
| XbaI | TCTAGA | 1 cut(s) | 130 |
| XspI | CTAG | 2 cut(s) | 131, 296 |
| ZraI | GACGTC | 2 cut(s) | 308, 472 |