Plant transposase (Ptta/En/Spm family)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 135 |
| AccB1I | GGYRCC | 1 cut(s) | 251 |
| AclWI | GGATC | 1 cut(s) | 189 |
| AcyI | GRCGYC | 1 cut(s) | 132 |
| AfaI | GTAC | 1 cut(s) | 399 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 127, 358, 384 |
| AgsI | TTSAA | 2 cut(s) | 292, 425 |
| AjnI | CCWGG | 4 cut(s) | 25, 177, 318, 351 |
| AluBI | AGCT | 1 cut(s) | 106 |
| AluI | AGCT | 1 cut(s) | 106 |
| Alw21I | GWGCWC | 1 cut(s) | 77 |
| Alw26I | GTCTC | 2 cut(s) | 110, 384 |
| AlwI | GGATC | 1 cut(s) | 189 |
| AoxI | GGCC | 3 cut(s) | 315, 348, 418 |
| ApaI | GGGCCC | 1 cut(s) | 352 |
| ApeKI | GCWGC | 1 cut(s) | 103 |
| AspS9I | GGNCC | 4 cut(s) | 316, 348, 349, 452 |
| AsuC2I | CCSGG | 2 cut(s) | 113, 379 |
| AvaII | GGWCC | 1 cut(s) | 452 |
| AxyI | CCTNAGG | 1 cut(s) | 144 |
| BaeGI | GKGCMC | 1 cut(s) | 352 |
| BanI | GGYRCC | 1 cut(s) | 251 |
| BanII | GRGCYC | 1 cut(s) | 352 |
| Bbv12I | GWGCWC | 1 cut(s) | 77 |
| BbvI | GCAGC | 1 cut(s) | 115 |
| BciT130I | CCWGG | 4 cut(s) | 27, 179, 320, 353 |
| BcnI | CCSGG | 2 cut(s) | 113, 379 |
| BcoDI | GTCTC | 2 cut(s) | 110, 384 |
| BfaI | CTAG | 1 cut(s) | 493 |
| BisI | GCNGC | 1 cut(s) | 104 |
| BlsI | GCNGC | 1 cut(s) | 105 |
| Bme1390I | CCNGG | 6 cut(s) | 27, 113, 179, 320, 353, 379 |
| Bme18I | GGWCC | 1 cut(s) | 452 |
| BmgT120I | GGNCC | 4 cut(s) | 316, 348, 349, 452 |
| BmiI | GGNNCC | 2 cut(s) | 253, 350 |
| BmrFI | CCNGG | 6 cut(s) | 27, 113, 179, 320, 353, 379 |
| BmsI | GCATC | 1 cut(s) | 274 |
| BoxI | GACNNNNGTC | 1 cut(s) | 173 |
| BpuMI | CCSGG | 2 cut(s) | 113, 379 |
| BsaHI | GRCGYC | 1 cut(s) | 132 |
| BsaI | GGTCTC | 2 cut(s) | 110, 384 |
| BsaJI | CCNNGG | 6 cut(s) | 111, 318, 319, 351, 352, 378 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 353, 383 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 127, 358, 384 |
| Bse21I | CCTNAGG | 1 cut(s) | 144 |
| BseBI | CCWGG | 4 cut(s) | 27, 179, 320, 353 |
| BseDI | CCNNGG | 6 cut(s) | 111, 318, 319, 351, 352, 378 |
| BseGI | GGATG | 1 cut(s) | 154 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 127, 358, 384 |
| BseMII | CTCAG | 4 cut(s) | 33, 135, 335, 480 |
| BseRI | GAGGAG | 1 cut(s) | 374 |
| BseSI | GKGCMC | 1 cut(s) | 352 |
| BseXI | GCAGC | 1 cut(s) | 115 |
| Bsh1285I | CGRYCG | 1 cut(s) | 126 |
| BshFI | GGCC | 3 cut(s) | 317, 350, 420 |
| BshNI | GGYRCC | 1 cut(s) | 251 |
| BsiEI | CGRYCG | 1 cut(s) | 126 |
| BsiHKAI | GWGCWC | 1 cut(s) | 77 |
| BsiSI | CCGG | 3 cut(s) | 113, 378, 417 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 127, 358, 384 |
| BsmAI | GTCTC | 2 cut(s) | 110, 384 |
| BsnI | GGCC | 3 cut(s) | 317, 350, 420 |
| Bso31I | GGTCTC | 2 cut(s) | 110, 384 |
| Bsp120I | GGGCCC | 1 cut(s) | 348 |
| Bsp1286I | GDGCHC | 2 cut(s) | 77, 352 |
| Bsp1407I | TGTACA | 1 cut(s) | 397 |
| Bsp143I | GATC | 4 cut(s) | 123, 162, 181, 266 |
| BspANI | GGCC | 3 cut(s) | 317, 350, 420 |
| BspCNI | CTCAG | 4 cut(s) | 34, 136, 336, 479 |
| BspHI | TCATGA | 1 cut(s) | 165 |
| BspLI | GGNNCC | 2 cut(s) | 253, 350 |
| BspPI | GGATC | 1 cut(s) | 189 |
| BspT107I | GGYRCC | 1 cut(s) | 251 |
| BspTNI | GGTCTC | 2 cut(s) | 110, 384 |
| BsrGI | TGTACA | 1 cut(s) | 397 |
| BssECI | CCNNGG | 6 cut(s) | 111, 318, 319, 351, 352, 378 |
| BssMI | GATC | 4 cut(s) | 123, 162, 181, 266 |
| BssNI | GRCGYC | 1 cut(s) | 132 |
| Bst2UI | CCWGG | 4 cut(s) | 27, 179, 320, 353 |
| Bst6I | CTCTTC | 1 cut(s) | 262 |
| BstACI | GRCGYC | 1 cut(s) | 132 |
| BstAUI | TGTACA | 1 cut(s) | 397 |
| BstC8I | GCNNGC | 1 cut(s) | 326 |
| BstDEI | CTNAG | 5 cut(s) | 42, 144, 190, 344, 466 |
| BstF5I | GGATG | 1 cut(s) | 154 |
| BstKTI | GATC | 4 cut(s) | 126, 165, 184, 269 |
| BstMAI | GTCTC | 2 cut(s) | 110, 384 |
| BstMBI | GATC | 4 cut(s) | 123, 162, 181, 266 |
| BstMCI | CGRYCG | 1 cut(s) | 126 |
| BstNI | CCWGG | 4 cut(s) | 27, 179, 320, 353 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 173 |
| BstSCI | CCNGG | 6 cut(s) | 25, 111, 177, 318, 351, 377 |
| BstSLI | GKGCMC | 1 cut(s) | 352 |
| BstV1I | GCAGC | 1 cut(s) | 115 |
| Bsu36I | CCTNAGG | 1 cut(s) | 144 |
| BsuRI | GGCC | 3 cut(s) | 317, 350, 420 |
| BtgZI | GCGATG | 1 cut(s) | 84 |
| BtsCI | GGATG | 1 cut(s) | 154 |
| Cac8I | GCNNGC | 1 cut(s) | 326 |
| CciI | TCATGA | 1 cut(s) | 165 |
| Cfr13I | GGNCC | 4 cut(s) | 316, 348, 349, 452 |
| CsiI | ACCWGGT | 1 cut(s) | 25 |
| Csp6I | GTAC | 1 cut(s) | 398 |
| CviAII | CATG | 2 cut(s) | 53, 166 |
| CviJI | RGCY | 6 cut(s) | 106, 317, 350, 404, 420, 472 |
| CviKI_1 | RGCY | 6 cut(s) | 106, 317, 350, 404, 420, 472 |
| CviQI | GTAC | 1 cut(s) | 398 |
| DdeI | CTNAG | 5 cut(s) | 42, 144, 190, 344, 466 |
| DpnI | GATC | 4 cut(s) | 125, 164, 183, 268 |
| DpnII | GATC | 4 cut(s) | 123, 162, 181, 266 |
| Eam1104I | CTCTTC | 1 cut(s) | 262 |
| EarI | CTCTTC | 1 cut(s) | 262 |
| Eco24I | GRGCYC | 1 cut(s) | 352 |
| Eco31I | GGTCTC | 2 cut(s) | 110, 384 |
| Eco47I | GGWCC | 1 cut(s) | 452 |
| Eco81I | CCTNAGG | 1 cut(s) | 144 |
| EcoO109I | RGGNCCY | 1 cut(s) | 348 |
| EcoRII | CCWGG | 4 cut(s) | 25, 177, 318, 351 |
| EcoT22I | ATGCAT | 1 cut(s) | 289 |
| EcoT38I | GRGCYC | 1 cut(s) | 352 |
| FaeI | CATG | 2 cut(s) | 56, 169 |
| FaiI | YATR | 7 cut(s) | 54, 62, 167, 224, 373, 375, 407 |
| FatI | CATG | 2 cut(s) | 52, 165 |
| FauNDI | CATATG | 1 cut(s) | 373 |
| Fnu4HI | GCNGC | 1 cut(s) | 104 |
| FokI | GGATG | 1 cut(s) | 161 |
| FriOI | GRGCYC | 1 cut(s) | 352 |
| Fsp4HI | GCNGC | 1 cut(s) | 104 |
| FspBI | CTAG | 1 cut(s) | 493 |
| GluI | GCNGC | 1 cut(s) | 104 |
| HaeIII | GGCC | 3 cut(s) | 317, 350, 420 |
| HapII | CCGG | 3 cut(s) | 113, 378, 417 |
| Hin1I | GRCGYC | 1 cut(s) | 132 |
| Hin1II | CATG | 2 cut(s) | 56, 169 |
| HinfI | GANTC | 1 cut(s) | 238 |
| HpaII | CCGG | 3 cut(s) | 113, 378, 417 |
| Hpy166II | GTNNAC | 2 cut(s) | 16, 455 |
| Hpy188I | TCNGA | 3 cut(s) | 162, 186, 469 |
| Hpy188III | TCNNGA | 5 cut(s) | 32, 136, 166, 242, 264 |
| Hpy8I | GTNNAC | 2 cut(s) | 16, 455 |
| Hpy99I | CGWCG | 2 cut(s) | 201, 204 |
| HpyAV | CCTTC | 4 cut(s) | 286, 328, 431, 468 |
| HpyCH4IV | ACGT | 3 cut(s) | 12, 18, 132 |
| HpyCH4V | TGCA | 1 cut(s) | 287 |
| HpyF3I | CTNAG | 5 cut(s) | 42, 144, 190, 344, 466 |
| HpySE526I | ACGT | 3 cut(s) | 12, 18, 132 |
| Hsp92I | GRCGYC | 1 cut(s) | 132 |
| Hsp92II | CATG | 2 cut(s) | 56, 169 |
| Kzo9I | GATC | 4 cut(s) | 123, 162, 181, 266 |
| LmnI | GCTCC | 1 cut(s) | 337 |
| Lsp1109I | GCAGC | 1 cut(s) | 115 |
| LweI | GCATC | 1 cut(s) | 274 |
| MabI | ACCWGGT | 1 cut(s) | 25 |
| MaeI | CTAG | 1 cut(s) | 493 |
| MaeII | ACGT | 3 cut(s) | 12, 18, 132 |
| MaeIII | GTNAC | 2 cut(s) | 133, 301 |
| MalI | GATC | 4 cut(s) | 125, 164, 183, 268 |
| MboI | GATC | 4 cut(s) | 123, 162, 181, 266 |
| MboII | GAAGA | 2 cut(s) | 249, 252 |
| MhlI | GDGCHC | 2 cut(s) | 77, 352 |
| Mph1103I | ATGCAT | 1 cut(s) | 289 |
| MslI | CAYNNNNRTG | 1 cut(s) | 410 |
| MspI | CCGG | 3 cut(s) | 113, 378, 417 |
| MspR9I | CCNGG | 6 cut(s) | 27, 113, 179, 320, 353, 379 |
| MvaI | CCWGG | 4 cut(s) | 27, 179, 320, 353 |
| NciI | CCSGG | 2 cut(s) | 113, 379 |
| NdeI | CATATG | 1 cut(s) | 373 |
| NdeII | GATC | 4 cut(s) | 123, 162, 181, 266 |
| NlaIII | CATG | 2 cut(s) | 56, 169 |
| NlaIV | GGNNCC | 2 cut(s) | 253, 350 |
| NmuCI | GTSAC | 2 cut(s) | 133, 301 |
| NsiI | ATGCAT | 1 cut(s) | 289 |
| PagI | TCATGA | 1 cut(s) | 165 |
| PasI | CCCWGGG | 2 cut(s) | 319, 352 |
| PfeI | GAWTC | 1 cut(s) | 238 |
| PflFI | GACNNNGTC | 1 cut(s) | 27 |
| PfoI | TCCNGGA | 1 cut(s) | 177 |
| PkrI | GCNGC | 1 cut(s) | 105 |
| Ple19I | CGATCG | 1 cut(s) | 126 |
| PshAI | GACNNNNGTC | 1 cut(s) | 173 |
| Psp6I | CCWGG | 4 cut(s) | 25, 177, 318, 351 |
| PspGI | CCWGG | 4 cut(s) | 25, 177, 318, 351 |
| PspN4I | GGNNCC | 2 cut(s) | 253, 350 |
| PspOMI | GGGCCC | 1 cut(s) | 348 |
| PspPI | GGNCC | 4 cut(s) | 316, 348, 349, 452 |
| PsyI | GACNNNGTC | 1 cut(s) | 27 |
| PvuI | CGATCG | 1 cut(s) | 126 |
| RsaI | GTAC | 1 cut(s) | 399 |
| RsaNI | GTAC | 1 cut(s) | 398 |
| RseI | CAYNNNNRTG | 1 cut(s) | 410 |
| SatI | GCNGC | 1 cut(s) | 104 |
| Sau3AI | GATC | 4 cut(s) | 123, 162, 181, 266 |
| Sau96I | GGNCC | 4 cut(s) | 316, 348, 349, 452 |
| ScrFI | CCNGG | 6 cut(s) | 27, 113, 179, 320, 353, 379 |
| SduI | GDGCHC | 2 cut(s) | 77, 352 |
| SexAI | ACCWGGT | 1 cut(s) | 25 |
| SfaNI | GCATC | 1 cut(s) | 274 |
| SinI | GGWCC | 1 cut(s) | 452 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 410 |
| SmlI | CTYRAG | 1 cut(s) | 5 |
| SmoI | CTYRAG | 1 cut(s) | 5 |
| SspMI | CTAG | 1 cut(s) | 493 |
| StyD4I | CCNGG | 6 cut(s) | 25, 111, 177, 318, 351, 377 |
| TaiI | ACGT | 3 cut(s) | 15, 21, 135 |
| TaqI | TCGA | 3 cut(s) | 22, 126, 461 |
| TatI | WGTACW | 1 cut(s) | 397 |
| TfiI | GAWTC | 1 cut(s) | 238 |
| TseFI | GTSAC | 2 cut(s) | 133, 301 |
| TseI | GCWGC | 1 cut(s) | 103 |
| Tsp45I | GTSAC | 2 cut(s) | 133, 301 |
| TspDTI | ATGAA | 3 cut(s) | 41, 347, 434 |
| Tth111I | GACNNNGTC | 1 cut(s) | 27 |
| VpaK11BI | GGWCC | 1 cut(s) | 452 |
| XspI | CTAG | 1 cut(s) | 493 |
| ZraI | GACGTC | 1 cut(s) | 133 |
| Zsp2I | ATGCAT | 1 cut(s) | 289 |