Plant transposase (Ptta/En/Spm family)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 205 |
| AccB1I | GGYRCC | 1 cut(s) | 205 |
| AclWI | GGATC | 1 cut(s) | 178 |
| AcsI | RAATTY | 1 cut(s) | 32 |
| AfaI | GTAC | 1 cut(s) | 207 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 193 |
| AflIII | ACRYGT | 1 cut(s) | 66 |
| AgsI | TTSAA | 2 cut(s) | 31, 73 |
| AluBI | AGCT | 2 cut(s) | 115, 172 |
| AluI | AGCT | 2 cut(s) | 115, 172 |
| Alw26I | GTCTC | 1 cut(s) | 95 |
| AlwI | GGATC | 1 cut(s) | 178 |
| ApeKI | GCWGC | 2 cut(s) | 112, 169 |
| ApoI | RAATTY | 1 cut(s) | 32 |
| Asp718I | GGTACC | 1 cut(s) | 205 |
| AsuC2I | CCSGG | 1 cut(s) | 179 |
| AxyI | CCTNAGG | 1 cut(s) | 210 |
| BanI | GGYRCC | 1 cut(s) | 205 |
| BbvI | GCAGC | 2 cut(s) | 124, 181 |
| BcnI | CCSGG | 1 cut(s) | 179 |
| BcoDI | GTCTC | 1 cut(s) | 95 |
| BisI | GCNGC | 2 cut(s) | 113, 170 |
| BlsI | GCNGC | 2 cut(s) | 114, 171 |
| Bme1390I | CCNGG | 1 cut(s) | 179 |
| BmiI | GGNNCC | 1 cut(s) | 207 |
| BmrFI | CCNGG | 1 cut(s) | 179 |
| BpuMI | CCSGG | 1 cut(s) | 179 |
| BsaBI | GATNNNNATC | 1 cut(s) | 188 |
| BsaI | GGTCTC | 1 cut(s) | 95 |
| BsaJI | CCNNGG | 1 cut(s) | 177 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 193 |
| Bse21I | CCTNAGG | 1 cut(s) | 210 |
| Bse8I | GATNNNNATC | 1 cut(s) | 188 |
| BseDI | CCNNGG | 1 cut(s) | 177 |
| BseGI | GGATG | 1 cut(s) | 220 |
| BseJI | GATNNNNATC | 1 cut(s) | 188 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 193 |
| BseMII | CTCAG | 1 cut(s) | 99 |
| BseXI | GCAGC | 2 cut(s) | 124, 181 |
| BshNI | GGYRCC | 1 cut(s) | 205 |
| BsiSI | CCGG | 1 cut(s) | 179 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 193 |
| BsmAI | GTCTC | 1 cut(s) | 95 |
| Bso31I | GGTCTC | 1 cut(s) | 95 |
| Bsp143I | GATC | 2 cut(s) | 183, 189 |
| BspCNI | CTCAG | 1 cut(s) | 100 |
| BspLI | GGNNCC | 1 cut(s) | 207 |
| BspPI | GGATC | 1 cut(s) | 178 |
| BspT107I | GGYRCC | 1 cut(s) | 205 |
| BspTNI | GGTCTC | 1 cut(s) | 95 |
| BssECI | CCNNGG | 1 cut(s) | 177 |
| BssMI | GATC | 2 cut(s) | 183, 189 |
| Bst4CI | ACNGT | 1 cut(s) | 205 |
| BstDEI | CTNAG | 3 cut(s) | 92, 108, 210 |
| BstF5I | GGATG | 1 cut(s) | 220 |
| BstKTI | GATC | 2 cut(s) | 186, 192 |
| BstMAI | GTCTC | 1 cut(s) | 95 |
| BstMBI | GATC | 2 cut(s) | 183, 189 |
| BstNSI | RCATGY | 1 cut(s) | 70 |
| BstSCI | CCNGG | 1 cut(s) | 177 |
| BstV1I | GCAGC | 2 cut(s) | 124, 181 |
| BstX2I | RGATCY | 1 cut(s) | 183 |
| BstYI | RGATCY | 1 cut(s) | 183 |
| Bsu36I | CCTNAGG | 1 cut(s) | 210 |
| BtgZI | GCGATG | 1 cut(s) | 150 |
| BtsCI | GGATG | 1 cut(s) | 220 |
| BtsI | GCAGTG | 1 cut(s) | 148 |
| BtsIMutI | CAGTG | 2 cut(s) | 105, 148 |
| Csp6I | GTAC | 1 cut(s) | 206 |
| CviAII | CATG | 2 cut(s) | 67, 119 |
| CviJI | RGCY | 2 cut(s) | 115, 172 |
| CviKI_1 | RGCY | 2 cut(s) | 115, 172 |
| CviQI | GTAC | 1 cut(s) | 206 |
| DdeI | CTNAG | 3 cut(s) | 92, 108, 210 |
| DpnI | GATC | 2 cut(s) | 185, 191 |
| DpnII | GATC | 2 cut(s) | 183, 189 |
| Eco31I | GGTCTC | 1 cut(s) | 95 |
| Eco81I | CCTNAGG | 1 cut(s) | 210 |
| FaeI | CATG | 2 cut(s) | 70, 122 |
| FaiI | YATR | 5 cut(s) | 20, 39, 68, 120, 128 |
| FatI | CATG | 2 cut(s) | 66, 118 |
| Fnu4HI | GCNGC | 2 cut(s) | 113, 170 |
| Fsp4HI | GCNGC | 2 cut(s) | 113, 170 |
| GluI | GCNGC | 2 cut(s) | 113, 170 |
| HapII | CCGG | 1 cut(s) | 179 |
| Hin1II | CATG | 2 cut(s) | 70, 122 |
| HpaII | CCGG | 1 cut(s) | 179 |
| Hpy188III | TCNNGA | 1 cut(s) | 187 |
| HpyCH4III | ACNGT | 1 cut(s) | 205 |
| HpyCH4IV | ACGT | 3 cut(s) | 5, 84, 198 |
| HpyF3I | CTNAG | 3 cut(s) | 92, 108, 210 |
| HpySE526I | ACGT | 3 cut(s) | 5, 84, 198 |
| Hsp92II | CATG | 2 cut(s) | 70, 122 |
| KpnI | GGTACC | 1 cut(s) | 209 |
| Kzo9I | GATC | 2 cut(s) | 183, 189 |
| LpnPI | CCDG | 1 cut(s) | 192 |
| Lsp1109I | GCAGC | 2 cut(s) | 124, 181 |
| MaeII | ACGT | 3 cut(s) | 5, 84, 198 |
| MaeIII | GTNAC | 2 cut(s) | 95, 199 |
| MalI | GATC | 2 cut(s) | 185, 191 |
| MboI | GATC | 2 cut(s) | 183, 189 |
| MflI | RGATCY | 1 cut(s) | 183 |
| MluCI | AATT | 2 cut(s) | 32, 42 |
| MseI | TTAA | 2 cut(s) | 23, 63 |
| MspI | CCGG | 1 cut(s) | 179 |
| MspR9I | CCNGG | 1 cut(s) | 179 |
| NciI | CCSGG | 1 cut(s) | 179 |
| NdeII | GATC | 2 cut(s) | 183, 189 |
| NlaIII | CATG | 2 cut(s) | 70, 122 |
| NlaIV | GGNNCC | 1 cut(s) | 207 |
| NspI | RCATGY | 1 cut(s) | 70 |
| PciI | ACATGT | 1 cut(s) | 66 |
| PkrI | GCNGC | 2 cut(s) | 114, 171 |
| PscI | ACATGT | 1 cut(s) | 66 |
| PspN4I | GGNNCC | 1 cut(s) | 207 |
| PsuI | RGATCY | 1 cut(s) | 183 |
| RsaI | GTAC | 1 cut(s) | 207 |
| RsaNI | GTAC | 1 cut(s) | 206 |
| SaqAI | TTAA | 2 cut(s) | 23, 63 |
| SatI | GCNGC | 2 cut(s) | 113, 170 |
| Sau3AI | GATC | 2 cut(s) | 183, 189 |
| ScrFI | CCNGG | 1 cut(s) | 179 |
| SetI | ASST | 8 cut(s) | 8, 13, 87, 117, 174, 201, 211, 223 |
| SgeI | CNNG | 9 cut(s) | 79, 85, 112, 131, 167, 190, 191, 199, 205 |
| Sse9I | AATT | 2 cut(s) | 32, 42 |
| StyD4I | CCNGG | 1 cut(s) | 177 |
| TaaI | ACNGT | 1 cut(s) | 205 |
| TaiI | ACGT | 3 cut(s) | 8, 87, 201 |
| TaqI | TCGA | 1 cut(s) | 88 |
| TasI | AATT | 2 cut(s) | 32, 42 |
| Tru1I | TTAA | 2 cut(s) | 23, 63 |
| Tru9I | TTAA | 2 cut(s) | 23, 63 |
| TscAI | CASTG | 2 cut(s) | 112, 148 |
| TseI | GCWGC | 2 cut(s) | 112, 169 |
| TspDTI | ATGAA | 1 cut(s) | 107 |
| TspRI | CASTG | 2 cut(s) | 112, 148 |
| XapI | RAATTY | 1 cut(s) | 32 |
| XceI | RCATGY | 1 cut(s) | 70 |