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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 11 |
| AclWI | GGATC | 1 cut(s) | 137 |
| AluBI | AGCT | 2 cut(s) | 139, 196 |
| AluI | AGCT | 2 cut(s) | 139, 196 |
| AlwI | GGATC | 1 cut(s) | 137 |
| AoxI | GGCC | 1 cut(s) | 64 |
| ApeKI | GCWGC | 1 cut(s) | 77 |
| AspLEI | GCGC | 2 cut(s) | 6, 77 |
| AsuHPI | GGTGA | 1 cut(s) | 11 |
| BbvI | GCAGC | 1 cut(s) | 64 |
| BccI | CCATC | 1 cut(s) | 30 |
| BfaI | CTAG | 2 cut(s) | 204, 226 |
| BfmI | CTRYAG | 1 cut(s) | 78 |
| BfoI | RGCGCY | 1 cut(s) | 78 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 72 |
| BisI | GCNGC | 1 cut(s) | 78 |
| BlsI | GCNGC | 1 cut(s) | 79 |
| BmiI | GGNNCC | 1 cut(s) | 92 |
| Bse1I | ACTGG | 1 cut(s) | 76 |
| BseGI | GGATG | 1 cut(s) | 115 |
| BseNI | ACTGG | 1 cut(s) | 76 |
| BseXI | GCAGC | 1 cut(s) | 64 |
| BshFI | GGCC | 1 cut(s) | 66 |
| BsnI | GGCC | 1 cut(s) | 66 |
| Bsp143I | GATC | 2 cut(s) | 142, 156 |
| BspACI | CCGC | 1 cut(s) | 11 |
| BspANI | GGCC | 1 cut(s) | 66 |
| BspLI | GGNNCC | 1 cut(s) | 92 |
| BspMAI | CTGCAG | 1 cut(s) | 82 |
| BspPI | GGATC | 1 cut(s) | 137 |
| BsrI | ACTGG | 1 cut(s) | 76 |
| BssMI | GATC | 2 cut(s) | 142, 156 |
| Bst4CI | ACNGT | 1 cut(s) | 62 |
| BstF5I | GGATG | 1 cut(s) | 115 |
| BstH2I | RGCGCY | 1 cut(s) | 78 |
| BstHHI | GCGC | 2 cut(s) | 6, 77 |
| BstKTI | GATC | 2 cut(s) | 145, 159 |
| BstMBI | GATC | 2 cut(s) | 142, 156 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 10, 72 |
| BstSFI | CTRYAG | 1 cut(s) | 78 |
| BstV1I | GCAGC | 1 cut(s) | 64 |
| BsuRI | GGCC | 1 cut(s) | 66 |
| BtsCI | GGATG | 1 cut(s) | 115 |
| BtsI | GCAGTG | 1 cut(s) | 87 |
| BtsIMutI | CAGTG | 3 cut(s) | 41, 69, 87 |
| CfoI | GCGC | 2 cut(s) | 6, 77 |
| CviJI | RGCY | 5 cut(s) | 66, 93, 122, 139, 196 |
| CviKI_1 | RGCY | 5 cut(s) | 66, 93, 122, 139, 196 |
| DpnI | GATC | 2 cut(s) | 144, 158 |
| DpnII | GATC | 2 cut(s) | 142, 156 |
| FauI | CCCGC | 1 cut(s) | 18 |
| Fnu4HI | GCNGC | 1 cut(s) | 78 |
| FokI | GGATG | 1 cut(s) | 102 |
| Fsp4HI | GCNGC | 1 cut(s) | 78 |
| FspBI | CTAG | 2 cut(s) | 204, 226 |
| GlaI | GCGC | 2 cut(s) | 5, 76 |
| GluI | GCNGC | 1 cut(s) | 78 |
| HaeII | RGCGCY | 1 cut(s) | 78 |
| HaeIII | GGCC | 1 cut(s) | 66 |
| HhaI | GCGC | 2 cut(s) | 6, 77 |
| Hin6I | GCGC | 2 cut(s) | 4, 75 |
| HinP1I | GCGC | 2 cut(s) | 4, 75 |
| HindIII | AAGCTT | 1 cut(s) | 137 |
| HinfI | GANTC | 1 cut(s) | 85 |
| HphI | GGTGA | 1 cut(s) | 11 |
| Hpy166II | GTNNAC | 1 cut(s) | 102 |
| Hpy188I | TCNGA | 1 cut(s) | 90 |
| Hpy8I | GTNNAC | 1 cut(s) | 102 |
| HpyAV | CCTTC | 2 cut(s) | 104, 179 |
| HpyCH4III | ACNGT | 1 cut(s) | 62 |
| HpyCH4IV | ACGT | 2 cut(s) | 28, 215 |
| HpyCH4V | TGCA | 2 cut(s) | 58, 80 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 10, 72 |
| HpySE526I | ACGT | 2 cut(s) | 28, 215 |
| HspAI | GCGC | 2 cut(s) | 4, 75 |
| Kzo9I | GATC | 2 cut(s) | 142, 156 |
| LmnI | GCTCC | 2 cut(s) | 90, 168 |
| LpnPI | CCDG | 3 cut(s) | 57, 132, 206 |
| Lsp1109I | GCAGC | 1 cut(s) | 64 |
| MaeI | CTAG | 2 cut(s) | 204, 226 |
| MaeII | ACGT | 2 cut(s) | 28, 215 |
| MalI | GATC | 2 cut(s) | 144, 158 |
| MboI | GATC | 2 cut(s) | 142, 156 |
| MluCI | AATT | 1 cut(s) | 105 |
| MnlI | CCTC | 5 cut(s) | 17, 77, 120, 142, 186 |
| MspA1I | CMGCKG | 1 cut(s) | 196 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 10, 72 |
| NdeII | GATC | 2 cut(s) | 142, 156 |
| NlaIV | GGNNCC | 1 cut(s) | 92 |
| PfeI | GAWTC | 1 cut(s) | 85 |
| PkrI | GCNGC | 1 cut(s) | 79 |
| PspN4I | GGNNCC | 1 cut(s) | 92 |
| PstI | CTGCAG | 1 cut(s) | 82 |
| PvuII | CAGCTG | 1 cut(s) | 196 |
| SatI | GCNGC | 1 cut(s) | 78 |
| Sau3AI | GATC | 2 cut(s) | 142, 156 |
| SetI | ASST | 9 cut(s) | 31, 141, 155, 171, 178, 198, 205, 218, 227 |
| SfcI | CTRYAG | 1 cut(s) | 78 |
| Sse9I | AATT | 1 cut(s) | 105 |
| SsiI | CCGC | 1 cut(s) | 11 |
| SspMI | CTAG | 2 cut(s) | 204, 226 |
| TaaI | ACNGT | 1 cut(s) | 62 |
| TaiI | ACGT | 2 cut(s) | 31, 218 |
| TaqI | TCGA | 1 cut(s) | 172 |
| TasI | AATT | 1 cut(s) | 105 |
| TfiI | GAWTC | 1 cut(s) | 85 |
| TscAI | CASTG | 3 cut(s) | 41, 76, 87 |
| TseI | GCWGC | 1 cut(s) | 77 |
| TspRI | CASTG | 3 cut(s) | 41, 76, 87 |
| XspI | CTAG | 2 cut(s) | 204, 226 |