Plant transposase (Ptta/En/Spm family)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 252 |
| Acc65I | GGTACC | 1 cut(s) | 256 |
| AccB1I | GGYRCC | 2 cut(s) | 256, 368 |
| AciI | CCGC | 1 cut(s) | 146 |
| AclWI | GGATC | 1 cut(s) | 306 |
| AcoI | YGGCCR | 2 cut(s) | 38, 322 |
| AcsI | RAATTY | 1 cut(s) | 163 |
| AcuI | CTGAAG | 1 cut(s) | 309 |
| AcyI | GRCGYC | 1 cut(s) | 249 |
| AfaI | GTAC | 1 cut(s) | 258 |
| AflII | CTTAAG | 1 cut(s) | 430 |
| AflIII | ACRYGT | 1 cut(s) | 107 |
| AjnI | CCWGG | 2 cut(s) | 132, 294 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 76, 108 |
| AluBI | AGCT | 3 cut(s) | 48, 223, 442 |
| AluI | AGCT | 3 cut(s) | 48, 223, 442 |
| Alw21I | GWGCWC | 1 cut(s) | 194 |
| Alw26I | GTCTC | 2 cut(s) | 227, 356 |
| AlwI | GGATC | 1 cut(s) | 306 |
| Ama87I | CYCGRG | 1 cut(s) | 49 |
| AoxI | GGCC | 3 cut(s) | 38, 322, 480 |
| ApeKI | GCWGC | 2 cut(s) | 212, 220 |
| ApoI | RAATTY | 1 cut(s) | 163 |
| Asp718I | GGTACC | 1 cut(s) | 256 |
| AspS9I | GGNCC | 1 cut(s) | 480 |
| AvaI | CYCGRG | 1 cut(s) | 49 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 165, 198 |
| BalI | TGGCCA | 2 cut(s) | 40, 324 |
| BanI | GGYRCC | 2 cut(s) | 256, 368 |
| Bbv12I | GWGCWC | 1 cut(s) | 194 |
| BbvI | GCAGC | 2 cut(s) | 224, 232 |
| BccI | CCATC | 1 cut(s) | 406 |
| BciT130I | CCWGG | 2 cut(s) | 134, 296 |
| BciVI | GTATCC | 1 cut(s) | 166 |
| BcoDI | GTCTC | 2 cut(s) | 227, 356 |
| BfaI | CTAG | 1 cut(s) | 484 |
| BfrI | CTTAAG | 1 cut(s) | 430 |
| BfuI | GTATCC | 1 cut(s) | 166 |
| BisI | GCNGC | 2 cut(s) | 213, 221 |
| BlpI | GCTNAGC | 1 cut(s) | 149 |
| BlsI | GCNGC | 2 cut(s) | 214, 222 |
| Bme1390I | CCNGG | 2 cut(s) | 134, 296 |
| BmeT110I | CYCGRG | 1 cut(s) | 49 |
| BmgT120I | GGNCC | 1 cut(s) | 480 |
| BmiI | GGNNCC | 2 cut(s) | 258, 370 |
| BmrFI | CCNGG | 2 cut(s) | 134, 296 |
| BmsI | GCATC | 2 cut(s) | 224, 394 |
| BoxI | GACNNNNGTC | 1 cut(s) | 290 |
| BpmI | CTGGAG | 1 cut(s) | 100 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 149 |
| BsaHI | GRCGYC | 1 cut(s) | 249 |
| BsaJI | CCNNGG | 2 cut(s) | 132, 307 |
| BseBI | CCWGG | 2 cut(s) | 134, 296 |
| BseDI | CCNNGG | 2 cut(s) | 132, 307 |
| BseGI | GGATG | 1 cut(s) | 271 |
| BseMII | CTCAG | 1 cut(s) | 140 |
| BseXI | GCAGC | 2 cut(s) | 224, 232 |
| Bsh1285I | CGRYCG | 1 cut(s) | 243 |
| BshFI | GGCC | 3 cut(s) | 40, 324, 482 |
| BshNI | GGYRCC | 2 cut(s) | 256, 368 |
| BsiEI | CGRYCG | 1 cut(s) | 243 |
| BsiHKAI | GWGCWC | 1 cut(s) | 194 |
| BsiHKCI | CYCGRG | 1 cut(s) | 49 |
| BsmAI | GTCTC | 2 cut(s) | 227, 356 |
| BsmBI | CGTCTC | 1 cut(s) | 356 |
| BsnI | GGCC | 3 cut(s) | 40, 324, 482 |
| BsoBI | CYCGRG | 1 cut(s) | 49 |
| Bsp1286I | GDGCHC | 1 cut(s) | 194 |
| Bsp143I | GATC | 5 cut(s) | 101, 240, 279, 298, 383 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 149 |
| BspACI | CCGC | 1 cut(s) | 146 |
| BspANI | GGCC | 3 cut(s) | 40, 324, 482 |
| BspCNI | CTCAG | 1 cut(s) | 141 |
| BspHI | TCATGA | 1 cut(s) | 282 |
| BspLI | GGNNCC | 2 cut(s) | 258, 370 |
| BspPI | GGATC | 1 cut(s) | 306 |
| BspQI | GCTCTTC | 1 cut(s) | 450 |
| BspT107I | GGYRCC | 2 cut(s) | 256, 368 |
| BspTI | CTTAAG | 1 cut(s) | 430 |
| BssECI | CCNNGG | 2 cut(s) | 132, 307 |
| BssMI | GATC | 5 cut(s) | 101, 240, 279, 298, 383 |
| BssNI | GRCGYC | 1 cut(s) | 249 |
| BssT1I | CCWWGG | 1 cut(s) | 307 |
| Bst2UI | CCWGG | 2 cut(s) | 134, 296 |
| Bst6I | CTCTTC | 2 cut(s) | 379, 450 |
| BstACI | GRCGYC | 1 cut(s) | 249 |
| BstAFI | CTTAAG | 1 cut(s) | 430 |
| BstC8I | GCNNGC | 2 cut(s) | 38, 444 |
| BstDEI | CTNAG | 2 cut(s) | 89, 149 |
| BstF5I | GGATG | 1 cut(s) | 271 |
| BstKTI | GATC | 5 cut(s) | 104, 243, 282, 301, 386 |
| BstMAI | GTCTC | 2 cut(s) | 227, 356 |
| BstMBI | GATC | 5 cut(s) | 101, 240, 279, 298, 383 |
| BstMCI | CGRYCG | 1 cut(s) | 243 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 448, 457 |
| BstNI | CCWGG | 2 cut(s) | 134, 296 |
| BstNSI | RCATGY | 1 cut(s) | 111 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 290 |
| BstSCI | CCNGG | 2 cut(s) | 132, 294 |
| BstV1I | GCAGC | 2 cut(s) | 224, 232 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 78 |
| BsuI | GTATCC | 1 cut(s) | 166 |
| BsuRI | GGCC | 3 cut(s) | 40, 324, 482 |
| BtgZI | GCGATG | 1 cut(s) | 201 |
| BtsCI | GGATG | 1 cut(s) | 271 |
| BtsIMutI | CAGTG | 1 cut(s) | 192 |
| Cac8I | GCNNGC | 2 cut(s) | 38, 444 |
| CciI | TCATGA | 1 cut(s) | 282 |
| Cfr13I | GGNCC | 1 cut(s) | 480 |
| Csp6I | GTAC | 1 cut(s) | 257 |
| CviAII | CATG | 4 cut(s) | 108, 253, 283, 320 |
| CviJI | RGCY | 6 cut(s) | 40, 48, 223, 324, 442, 482 |
| CviKI_1 | RGCY | 6 cut(s) | 40, 48, 223, 324, 442, 482 |
| CviQI | GTAC | 1 cut(s) | 257 |
| DdeI | CTNAG | 2 cut(s) | 89, 149 |
| DpnI | GATC | 5 cut(s) | 103, 242, 281, 300, 385 |
| DpnII | GATC | 5 cut(s) | 101, 240, 279, 298, 383 |
| EaeI | YGGCCR | 2 cut(s) | 38, 322 |
| Eam1104I | CTCTTC | 2 cut(s) | 379, 450 |
| EarI | CTCTTC | 2 cut(s) | 379, 450 |
| Eco130I | CCWWGG | 1 cut(s) | 307 |
| Eco57I | CTGAAG | 1 cut(s) | 309 |
| Eco88I | CYCGRG | 1 cut(s) | 49 |
| EcoO109I | RGGNCCY | 1 cut(s) | 480 |
| EcoRII | CCWGG | 2 cut(s) | 132, 294 |
| EcoT14I | CCWWGG | 1 cut(s) | 307 |
| EcoT22I | ATGCAT | 1 cut(s) | 409 |
| ErhI | CCWWGG | 1 cut(s) | 307 |
| Esp3I | CGTCTC | 1 cut(s) | 356 |
| FaeI | CATG | 4 cut(s) | 111, 256, 286, 323 |
| FaiI | YATR | 8 cut(s) | 6, 109, 161, 179, 254, 284, 321, 341 |
| FatI | CATG | 4 cut(s) | 107, 252, 282, 319 |
| Fnu4HI | GCNGC | 2 cut(s) | 213, 221 |
| FokI | GGATG | 1 cut(s) | 278 |
| Fsp4HI | GCNGC | 2 cut(s) | 213, 221 |
| FspBI | CTAG | 1 cut(s) | 484 |
| GluI | GCNGC | 2 cut(s) | 213, 221 |
| GsuI | CTGGAG | 1 cut(s) | 100 |
| HaeIII | GGCC | 3 cut(s) | 40, 324, 482 |
| Hin1I | GRCGYC | 1 cut(s) | 249 |
| Hin1II | CATG | 4 cut(s) | 111, 256, 286, 323 |
| HindIII | AAGCTT | 1 cut(s) | 440 |
| HinfI | GANTC | 2 cut(s) | 54, 85 |
| Hpy166II | GTNNAC | 1 cut(s) | 123 |
| Hpy188I | TCNGA | 2 cut(s) | 279, 303 |
| Hpy188III | TCNNGA | 5 cut(s) | 51, 79, 283, 359, 381 |
| Hpy8I | GTNNAC | 1 cut(s) | 123 |
| Hpy99I | CGWCG | 1 cut(s) | 318 |
| HpyCH4IV | ACGT | 2 cut(s) | 119, 249 |
| HpyCH4V | TGCA | 2 cut(s) | 407, 451 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 448, 457 |
| HpyF3I | CTNAG | 2 cut(s) | 89, 149 |
| HpySE526I | ACGT | 2 cut(s) | 119, 249 |
| Hsp92I | GRCGYC | 1 cut(s) | 249 |
| Hsp92II | CATG | 4 cut(s) | 111, 256, 286, 323 |
| KpnI | GGTACC | 1 cut(s) | 260 |
| Kzo9I | GATC | 5 cut(s) | 101, 240, 279, 298, 383 |
| LguI | GCTCTTC | 1 cut(s) | 450 |
| LmnI | GCTCC | 1 cut(s) | 469 |
| Lsp1109I | GCAGC | 2 cut(s) | 224, 232 |
| LweI | GCATC | 2 cut(s) | 224, 394 |
| MaeI | CTAG | 1 cut(s) | 484 |
| MaeII | ACGT | 2 cut(s) | 119, 249 |
| MaeIII | GTNAC | 1 cut(s) | 421 |
| MalI | GATC | 5 cut(s) | 103, 242, 281, 300, 385 |
| MboI | GATC | 5 cut(s) | 101, 240, 279, 298, 383 |
| MboII | GAAGA | 4 cut(s) | 35, 366, 369, 467 |
| MhlI | GDGCHC | 1 cut(s) | 194 |
| MlsI | TGGCCA | 2 cut(s) | 40, 324 |
| MluCI | AATT | 1 cut(s) | 163 |
| MluNI | TGGCCA | 2 cut(s) | 40, 324 |
| MlyI | GAGTC | 1 cut(s) | 63 |
| MnlI | CCTC | 8 cut(s) | 75, 238, 256, 382, 391, 409, 459, 471 |
| Mox20I | TGGCCA | 2 cut(s) | 40, 324 |
| Mph1103I | ATGCAT | 1 cut(s) | 409 |
| MscI | TGGCCA | 2 cut(s) | 40, 324 |
| MseI | TTAA | 3 cut(s) | 17, 113, 431 |
| Msp20I | TGGCCA | 2 cut(s) | 40, 324 |
| MspA1I | CMGCKG | 1 cut(s) | 148 |
| MspCI | CTTAAG | 1 cut(s) | 430 |
| MspR9I | CCNGG | 2 cut(s) | 134, 296 |
| MvaI | CCWGG | 2 cut(s) | 134, 296 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 448, 457 |
| NdeII | GATC | 5 cut(s) | 101, 240, 279, 298, 383 |
| NlaIII | CATG | 4 cut(s) | 111, 256, 286, 323 |
| NlaIV | GGNNCC | 2 cut(s) | 258, 370 |
| NmuCI | GTSAC | 1 cut(s) | 421 |
| NsiI | ATGCAT | 1 cut(s) | 409 |
| NspI | RCATGY | 1 cut(s) | 111 |
| PagI | TCATGA | 1 cut(s) | 282 |
| PciI | ACATGT | 1 cut(s) | 107 |
| PciSI | GCTCTTC | 1 cut(s) | 450 |
| PfeI | GAWTC | 1 cut(s) | 85 |
| PfoI | TCCNGGA | 1 cut(s) | 294 |
| PkrI | GCNGC | 2 cut(s) | 214, 222 |
| Ple19I | CGATCG | 1 cut(s) | 243 |
| PleI | GAGTC | 1 cut(s) | 62 |
| PpsI | GAGTC | 1 cut(s) | 62 |
| PscI | ACATGT | 1 cut(s) | 107 |
| PshAI | GACNNNNGTC | 1 cut(s) | 290 |
| Psp6I | CCWGG | 2 cut(s) | 132, 294 |
| PspGI | CCWGG | 2 cut(s) | 132, 294 |
| PspN4I | GGNNCC | 2 cut(s) | 258, 370 |
| PspPI | GGNCC | 1 cut(s) | 480 |
| PvuI | CGATCG | 1 cut(s) | 243 |
| RsaI | GTAC | 1 cut(s) | 258 |
| RsaNI | GTAC | 1 cut(s) | 257 |
| SapI | GCTCTTC | 1 cut(s) | 450 |
| SaqAI | TTAA | 3 cut(s) | 17, 113, 431 |
| SatI | GCNGC | 2 cut(s) | 213, 221 |
| Sau3AI | GATC | 5 cut(s) | 101, 240, 279, 298, 383 |
| Sau96I | GGNCC | 1 cut(s) | 480 |
| SchI | GAGTC | 1 cut(s) | 63 |
| ScrFI | CCNGG | 2 cut(s) | 134, 296 |
| SduI | GDGCHC | 1 cut(s) | 194 |
| SetI | ASST | 9 cut(s) | 50, 122, 225, 252, 274, 309, 402, 431, 444 |
| SfaNI | GCATC | 2 cut(s) | 224, 394 |
| SmlI | CTYRAG | 1 cut(s) | 430 |
| SmoI | CTYRAG | 1 cut(s) | 430 |
| Sse9I | AATT | 1 cut(s) | 163 |
| SsiI | CCGC | 1 cut(s) | 146 |
| SspMI | CTAG | 1 cut(s) | 484 |
| StyD4I | CCNGG | 2 cut(s) | 132, 294 |
| StyI | CCWWGG | 1 cut(s) | 307 |
| TaiI | ACGT | 2 cut(s) | 122, 252 |
| TaqI | TCGA | 4 cut(s) | 104, 239, 243, 386 |
| TasI | AATT | 1 cut(s) | 163 |
| TfiI | GAWTC | 1 cut(s) | 85 |
| Tru1I | TTAA | 3 cut(s) | 17, 113, 431 |
| Tru9I | TTAA | 3 cut(s) | 17, 113, 431 |
| TscAI | CASTG | 1 cut(s) | 199 |
| TseFI | GTSAC | 1 cut(s) | 421 |
| TseI | GCWGC | 2 cut(s) | 212, 220 |
| Tsp45I | GTSAC | 1 cut(s) | 421 |
| TspDTI | ATGAA | 2 cut(s) | 155, 176 |
| TspRI | CASTG | 1 cut(s) | 199 |
| Vha464I | CTTAAG | 1 cut(s) | 430 |
| XapI | RAATTY | 1 cut(s) | 163 |
| XceI | RCATGY | 1 cut(s) | 111 |
| XspI | CTAG | 1 cut(s) | 484 |
| ZraI | GACGTC | 1 cut(s) | 250 |
| Zsp2I | ATGCAT | 1 cut(s) | 409 |