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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 143 |
| AclWI | GGATC | 2 cut(s) | 290, 326 |
| AcsI | RAATTY | 1 cut(s) | 249 |
| AfaI | GTAC | 1 cut(s) | 114 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 89, 143 |
| AgsI | TTSAA | 3 cut(s) | 254, 265, 308 |
| AjnI | CCWGG | 1 cut(s) | 324 |
| AluBI | AGCT | 3 cut(s) | 156, 292, 319 |
| AluI | AGCT | 3 cut(s) | 156, 292, 319 |
| Alw26I | GTCTC | 1 cut(s) | 164 |
| AlwI | GGATC | 2 cut(s) | 290, 326 |
| AoxI | GGCC | 1 cut(s) | 18 |
| ApeKI | GCWGC | 1 cut(s) | 153 |
| ApoI | RAATTY | 1 cut(s) | 249 |
| AspLEI | GCGC | 1 cut(s) | 313 |
| AsuHPI | GGTGA | 1 cut(s) | 314 |
| AxyI | CCTNAGG | 1 cut(s) | 84 |
| BbvI | GCAGC | 1 cut(s) | 165 |
| BciT130I | CCWGG | 1 cut(s) | 326 |
| BcoDI | GTCTC | 1 cut(s) | 164 |
| BfoI | RGCGCY | 1 cut(s) | 314 |
| BisI | GCNGC | 1 cut(s) | 154 |
| BlsI | GCNGC | 1 cut(s) | 155 |
| Bme1390I | CCNGG | 1 cut(s) | 326 |
| BmrFI | CCNGG | 1 cut(s) | 326 |
| BmsI | GCATC | 1 cut(s) | 55 |
| BpmI | CTGGAG | 2 cut(s) | 166, 221 |
| BpuEI | CTTGAG | 1 cut(s) | 242 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 89, 143 |
| Bse1I | ACTGG | 2 cut(s) | 137, 204 |
| Bse21I | CCTNAGG | 1 cut(s) | 84 |
| BseBI | CCWGG | 1 cut(s) | 326 |
| BseGI | GGATG | 1 cut(s) | 46 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 89, 143 |
| BseMII | CTCAG | 1 cut(s) | 75 |
| BseNI | ACTGG | 2 cut(s) | 137, 204 |
| BseRI | GAGGAG | 1 cut(s) | 29 |
| BseXI | GCAGC | 1 cut(s) | 165 |
| BshFI | GGCC | 1 cut(s) | 20 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 89, 143 |
| BsmAI | GTCTC | 1 cut(s) | 164 |
| BsnI | GGCC | 1 cut(s) | 20 |
| Bsp143I | GATC | 2 cut(s) | 295, 331 |
| BspANI | GGCC | 1 cut(s) | 20 |
| BspCNI | CTCAG | 1 cut(s) | 76 |
| BspPI | GGATC | 2 cut(s) | 290, 326 |
| BsrI | ACTGG | 2 cut(s) | 137, 204 |
| BssMI | GATC | 2 cut(s) | 295, 331 |
| Bst2UI | CCWGG | 1 cut(s) | 326 |
| BstDEI | CTNAG | 2 cut(s) | 84, 284 |
| BstF5I | GGATG | 1 cut(s) | 46 |
| BstH2I | RGCGCY | 1 cut(s) | 314 |
| BstHHI | GCGC | 1 cut(s) | 313 |
| BstKTI | GATC | 2 cut(s) | 298, 334 |
| BstMAI | GTCTC | 1 cut(s) | 164 |
| BstMBI | GATC | 2 cut(s) | 295, 331 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 242 |
| BstNI | CCWGG | 1 cut(s) | 326 |
| BstSCI | CCNGG | 1 cut(s) | 324 |
| BstV1I | GCAGC | 1 cut(s) | 165 |
| Bsu36I | CCTNAGG | 1 cut(s) | 84 |
| BsuRI | GGCC | 1 cut(s) | 20 |
| BtsCI | GGATG | 1 cut(s) | 46 |
| CfoI | GCGC | 1 cut(s) | 313 |
| CsiI | ACCWGGT | 1 cut(s) | 324 |
| Csp6I | GTAC | 1 cut(s) | 113 |
| CviJI | RGCY | 6 cut(s) | 20, 33, 156, 245, 292, 319 |
| CviKI_1 | RGCY | 6 cut(s) | 20, 33, 156, 245, 292, 319 |
| CviQI | GTAC | 1 cut(s) | 113 |
| DdeI | CTNAG | 2 cut(s) | 84, 284 |
| DpnI | GATC | 2 cut(s) | 297, 333 |
| DpnII | GATC | 2 cut(s) | 295, 331 |
| DraI | TTTAAA | 1 cut(s) | 105 |
| Eco147I | AGGCCT | 1 cut(s) | 20 |
| Eco81I | CCTNAGG | 1 cut(s) | 84 |
| EcoRII | CCWGG | 1 cut(s) | 324 |
| FaiI | YATR | 3 cut(s) | 191, 213, 276 |
| Fnu4HI | GCNGC | 1 cut(s) | 154 |
| FokI | GGATG | 1 cut(s) | 33 |
| Fsp4HI | GCNGC | 1 cut(s) | 154 |
| GlaI | GCGC | 1 cut(s) | 312 |
| GluI | GCNGC | 1 cut(s) | 154 |
| GsuI | CTGGAG | 2 cut(s) | 166, 221 |
| HaeII | RGCGCY | 1 cut(s) | 314 |
| HaeIII | GGCC | 1 cut(s) | 20 |
| HhaI | GCGC | 1 cut(s) | 313 |
| Hin6I | GCGC | 1 cut(s) | 311 |
| HinP1I | GCGC | 1 cut(s) | 311 |
| HinfI | GANTC | 1 cut(s) | 4 |
| HphI | GGTGA | 1 cut(s) | 314 |
| Hpy188I | TCNGA | 1 cut(s) | 9 |
| Hpy188III | TCNNGA | 1 cut(s) | 50 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 242 |
| HpyF3I | CTNAG | 2 cut(s) | 84, 284 |
| HspAI | GCGC | 1 cut(s) | 311 |
| Kzo9I | GATC | 2 cut(s) | 295, 331 |
| LpnPI | CCDG | 7 cut(s) | 63, 96, 150, 185, 196, 311, 338 |
| Lsp1109I | GCAGC | 1 cut(s) | 165 |
| LweI | GCATC | 1 cut(s) | 55 |
| MabI | ACCWGGT | 1 cut(s) | 324 |
| MalI | GATC | 2 cut(s) | 297, 333 |
| MboI | GATC | 2 cut(s) | 295, 331 |
| MluCI | AATT | 3 cut(s) | 237, 249, 301 |
| MnlI | CCTC | 9 cut(s) | 7, 10, 79, 103, 187, 190, 196, 219, 225 |
| MseI | TTAA | 2 cut(s) | 104, 343 |
| MspR9I | CCNGG | 1 cut(s) | 326 |
| MvaI | CCWGG | 1 cut(s) | 326 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 242 |
| NdeII | GATC | 2 cut(s) | 295, 331 |
| PceI | AGGCCT | 1 cut(s) | 20 |
| PfeI | GAWTC | 1 cut(s) | 4 |
| PflFI | GACNNNGTC | 1 cut(s) | 285 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 143 |
| PkrI | GCNGC | 1 cut(s) | 155 |
| Psp6I | CCWGG | 1 cut(s) | 324 |
| PspGI | CCWGG | 1 cut(s) | 324 |
| PsyI | GACNNNGTC | 1 cut(s) | 285 |
| RsaI | GTAC | 1 cut(s) | 114 |
| RsaNI | GTAC | 1 cut(s) | 113 |
| SaqAI | TTAA | 2 cut(s) | 104, 343 |
| SatI | GCNGC | 1 cut(s) | 154 |
| Sau3AI | GATC | 2 cut(s) | 295, 331 |
| ScrFI | CCNGG | 1 cut(s) | 326 |
| SetI | ASST | 7 cut(s) | 90, 114, 158, 182, 294, 321, 327 |
| SexAI | ACCWGGT | 1 cut(s) | 324 |
| SfaNI | GCATC | 1 cut(s) | 55 |
| SmlI | CTYRAG | 1 cut(s) | 221 |
| SmoI | CTYRAG | 1 cut(s) | 221 |
| Sse9I | AATT | 3 cut(s) | 237, 249, 301 |
| SseBI | AGGCCT | 1 cut(s) | 20 |
| SspI | AATATT | 1 cut(s) | 340 |
| StuI | AGGCCT | 1 cut(s) | 20 |
| StyD4I | CCNGG | 1 cut(s) | 324 |
| TasI | AATT | 3 cut(s) | 237, 249, 301 |
| TfiI | GAWTC | 1 cut(s) | 4 |
| Tru1I | TTAA | 2 cut(s) | 104, 343 |
| Tru9I | TTAA | 2 cut(s) | 104, 343 |
| TseI | GCWGC | 1 cut(s) | 153 |
| TspDTI | ATGAA | 1 cut(s) | 291 |
| Tth111I | GACNNNGTC | 1 cut(s) | 285 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 143 |
| XapI | RAATTY | 1 cut(s) | 249 |