Plant transposase (Ptta/En/Spm family)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 75 |
| AccB1I | GGYRCC | 1 cut(s) | 191 |
| AciI | CCGC | 2 cut(s) | 173, 269 |
| AclWI | GGATC | 2 cut(s) | 129, 343 |
| AcvI | CACGTG | 1 cut(s) | 365 |
| AcyI | GRCGYC | 1 cut(s) | 72 |
| AfaI | GTAC | 3 cut(s) | 81, 284, 321 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 67 |
| AflIII | ACRYGT | 1 cut(s) | 362 |
| AgsI | TTSAA | 2 cut(s) | 235, 384 |
| AjnI | CCWGG | 2 cut(s) | 273, 294 |
| AluBI | AGCT | 3 cut(s) | 46, 265, 407 |
| AluI | AGCT | 3 cut(s) | 46, 265, 407 |
| Alw26I | GTCTC | 2 cut(s) | 50, 179 |
| AlwI | GGATC | 2 cut(s) | 129, 343 |
| Ama87I | CYCGRG | 1 cut(s) | 83 |
| AoxI | GGCC | 3 cut(s) | 270, 291, 340 |
| ApeKI | GCWGC | 1 cut(s) | 43 |
| AspS9I | GGNCC | 2 cut(s) | 271, 292 |
| AsuC2I | CCSGG | 1 cut(s) | 53 |
| AsuHPI | GGTGA | 1 cut(s) | 392 |
| AvaI | CYCGRG | 1 cut(s) | 83 |
| BanI | GGYRCC | 1 cut(s) | 191 |
| BbrPI | CACGTG | 1 cut(s) | 365 |
| BbvI | GCAGC | 1 cut(s) | 55 |
| BciT130I | CCWGG | 2 cut(s) | 275, 296 |
| BcnI | CCSGG | 1 cut(s) | 53 |
| BcoDI | GTCTC | 2 cut(s) | 50, 179 |
| BisI | GCNGC | 2 cut(s) | 44, 270 |
| BlsI | GCNGC | 2 cut(s) | 45, 271 |
| Bme1390I | CCNGG | 3 cut(s) | 53, 275, 296 |
| BmeT110I | CYCGRG | 1 cut(s) | 83 |
| BmgT120I | GGNCC | 2 cut(s) | 271, 292 |
| BmiI | GGNNCC | 1 cut(s) | 193 |
| BmrFI | CCNGG | 3 cut(s) | 53, 275, 296 |
| BmrI | ACTGGG | 1 cut(s) | 409 |
| BmsI | GCATC | 2 cut(s) | 79, 345 |
| BmuI | ACTGGG | 1 cut(s) | 409 |
| BoxI | GACNNNNGTC | 1 cut(s) | 113 |
| BpmI | CTGGAG | 1 cut(s) | 329 |
| Bpu10I | CCTNAGC | 1 cut(s) | 328 |
| BpuMI | CCSGG | 1 cut(s) | 53 |
| BsaAI | YACGTR | 1 cut(s) | 365 |
| BsaHI | GRCGYC | 1 cut(s) | 72 |
| BsaI | GGTCTC | 1 cut(s) | 50 |
| BsaJI | CCNNGG | 5 cut(s) | 51, 84, 273, 274, 294 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 67 |
| Bse1I | ACTGG | 1 cut(s) | 415 |
| BseBI | CCWGG | 2 cut(s) | 275, 296 |
| BseDI | CCNNGG | 5 cut(s) | 51, 84, 273, 274, 294 |
| BseGI | GGATG | 2 cut(s) | 94, 312 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 67 |
| BseMII | CTCAG | 1 cut(s) | 102 |
| BseNI | ACTGG | 1 cut(s) | 415 |
| BseRI | GAGGAG | 1 cut(s) | 187 |
| BseXI | GCAGC | 1 cut(s) | 55 |
| BseYI | CCCAGC | 2 cut(s) | 390, 403 |
| Bsh1285I | CGRYCG | 1 cut(s) | 66 |
| BshFI | GGCC | 3 cut(s) | 272, 293, 342 |
| BshNI | GGYRCC | 1 cut(s) | 191 |
| BsiEI | CGRYCG | 1 cut(s) | 66 |
| BsiHKCI | CYCGRG | 1 cut(s) | 83 |
| BsiSI | CCGG | 2 cut(s) | 53, 339 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 67 |
| BsmAI | GTCTC | 2 cut(s) | 50, 179 |
| BsmBI | CGTCTC | 1 cut(s) | 179 |
| BsnI | GGCC | 3 cut(s) | 272, 293, 342 |
| Bso31I | GGTCTC | 1 cut(s) | 50 |
| BsoBI | CYCGRG | 1 cut(s) | 83 |
| Bsp1407I | TGTACA | 1 cut(s) | 319 |
| Bsp143I | GATC | 5 cut(s) | 63, 102, 121, 206, 348 |
| BspACI | CCGC | 2 cut(s) | 173, 269 |
| BspANI | GGCC | 3 cut(s) | 272, 293, 342 |
| BspCNI | CTCAG | 1 cut(s) | 103 |
| BspLI | GGNNCC | 1 cut(s) | 193 |
| BspPI | GGATC | 2 cut(s) | 129, 343 |
| BspT107I | GGYRCC | 1 cut(s) | 191 |
| BspTNI | GGTCTC | 1 cut(s) | 50 |
| BsrGI | TGTACA | 1 cut(s) | 319 |
| BsrI | ACTGG | 1 cut(s) | 415 |
| BssECI | CCNNGG | 5 cut(s) | 51, 84, 273, 274, 294 |
| BssMI | GATC | 5 cut(s) | 63, 102, 121, 206, 348 |
| BssNI | GRCGYC | 1 cut(s) | 72 |
| Bst2UI | CCWGG | 2 cut(s) | 275, 296 |
| Bst6I | CTCTTC | 1 cut(s) | 205 |
| BstACI | GRCGYC | 1 cut(s) | 72 |
| BstAUI | TGTACA | 1 cut(s) | 319 |
| BstBAI | YACGTR | 1 cut(s) | 365 |
| BstC8I | GCNNGC | 1 cut(s) | 267 |
| BstDEI | CTNAG | 2 cut(s) | 111, 328 |
| BstF5I | GGATG | 2 cut(s) | 94, 312 |
| BstKTI | GATC | 5 cut(s) | 66, 105, 124, 209, 351 |
| BstMAI | GTCTC | 2 cut(s) | 50, 179 |
| BstMBI | GATC | 5 cut(s) | 63, 102, 121, 206, 348 |
| BstMCI | CGRYCG | 1 cut(s) | 66 |
| BstNI | CCWGG | 2 cut(s) | 275, 296 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 113 |
| BstSCI | CCNGG | 3 cut(s) | 51, 273, 294 |
| BstV1I | GCAGC | 1 cut(s) | 55 |
| BstX2I | RGATCY | 1 cut(s) | 348 |
| BstYI | RGATCY | 1 cut(s) | 348 |
| BsuRI | GGCC | 3 cut(s) | 272, 293, 342 |
| BtgZI | GCGATG | 2 cut(s) | 24, 347 |
| BtsCI | GGATG | 2 cut(s) | 94, 312 |
| BtsI | GCAGTG | 1 cut(s) | 15 |
| BtsIMutI | CAGTG | 1 cut(s) | 15 |
| Cac8I | GCNNGC | 1 cut(s) | 267 |
| Cfr13I | GGNCC | 2 cut(s) | 271, 292 |
| Csp6I | GTAC | 3 cut(s) | 80, 283, 320 |
| CviJI | RGCY | 8 cut(s) | 46, 265, 272, 293, 326, 342, 394, 407 |
| CviKI_1 | RGCY | 8 cut(s) | 46, 265, 272, 293, 326, 342, 394, 407 |
| CviQI | GTAC | 3 cut(s) | 80, 283, 320 |
| DdeI | CTNAG | 2 cut(s) | 111, 328 |
| DpnI | GATC | 5 cut(s) | 65, 104, 123, 208, 350 |
| DpnII | GATC | 5 cut(s) | 63, 102, 121, 206, 348 |
| Eam1104I | CTCTTC | 1 cut(s) | 205 |
| EarI | CTCTTC | 1 cut(s) | 205 |
| Eco31I | GGTCTC | 1 cut(s) | 50 |
| Eco72I | CACGTG | 1 cut(s) | 365 |
| Eco88I | CYCGRG | 1 cut(s) | 83 |
| EcoRII | CCWGG | 2 cut(s) | 273, 294 |
| Esp3I | CGTCTC | 1 cut(s) | 179 |
| FaiI | YATR | 3 cut(s) | 144, 164, 314 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 249, 281 |
| FauI | CCCGC | 1 cut(s) | 166 |
| Fnu4HI | GCNGC | 2 cut(s) | 44, 270 |
| FokI | GGATG | 2 cut(s) | 101, 319 |
| Fsp4HI | GCNGC | 2 cut(s) | 44, 270 |
| GluI | GCNGC | 2 cut(s) | 44, 270 |
| GsaI | CCCAGC | 2 cut(s) | 394, 407 |
| GsuI | CTGGAG | 1 cut(s) | 329 |
| HaeIII | GGCC | 3 cut(s) | 272, 293, 342 |
| HapII | CCGG | 2 cut(s) | 53, 339 |
| Hin1I | GRCGYC | 1 cut(s) | 72 |
| HpaII | CCGG | 2 cut(s) | 53, 339 |
| HphI | GGTGA | 1 cut(s) | 392 |
| Hpy166II | GTNNAC | 2 cut(s) | 15, 213 |
| Hpy188I | TCNGA | 2 cut(s) | 102, 126 |
| Hpy188III | TCNNGA | 5 cut(s) | 106, 182, 204, 346, 352 |
| Hpy8I | GTNNAC | 2 cut(s) | 15, 213 |
| Hpy99I | CGWCG | 1 cut(s) | 141 |
| HpyAV | CCTTC | 3 cut(s) | 163, 229, 390 |
| HpyCH4IV | ACGT | 2 cut(s) | 72, 364 |
| HpyCH4V | TGCA | 1 cut(s) | 92 |
| HpyF3I | CTNAG | 2 cut(s) | 111, 328 |
| HpySE526I | ACGT | 2 cut(s) | 72, 364 |
| Hsp92I | GRCGYC | 1 cut(s) | 72 |
| Kzo9I | GATC | 5 cut(s) | 63, 102, 121, 206, 348 |
| LmnI | GCTCC | 1 cut(s) | 379 |
| Lsp1109I | GCAGC | 1 cut(s) | 55 |
| LweI | GCATC | 2 cut(s) | 79, 345 |
| MaeII | ACGT | 2 cut(s) | 72, 364 |
| MaeIII | GTNAC | 2 cut(s) | 106, 244 |
| MalI | GATC | 5 cut(s) | 65, 104, 123, 208, 350 |
| MboI | GATC | 5 cut(s) | 63, 102, 121, 206, 348 |
| MboII | GAAGA | 1 cut(s) | 192 |
| MflI | RGATCY | 1 cut(s) | 348 |
| MnlI | CCTC | 9 cut(s) | 61, 79, 106, 205, 208, 214, 232, 297, 353 |
| MseI | TTAA | 1 cut(s) | 288 |
| MspA1I | CMGCKG | 1 cut(s) | 407 |
| MspI | CCGG | 2 cut(s) | 53, 339 |
| MspR9I | CCNGG | 3 cut(s) | 53, 275, 296 |
| MvaI | CCWGG | 2 cut(s) | 275, 296 |
| NciI | CCSGG | 1 cut(s) | 53 |
| NdeII | GATC | 5 cut(s) | 63, 102, 121, 206, 348 |
| NlaIV | GGNNCC | 1 cut(s) | 193 |
| NmuCI | GTSAC | 2 cut(s) | 106, 244 |
| PasI | CCCWGGG | 1 cut(s) | 274 |
| PkrI | GCNGC | 2 cut(s) | 45, 271 |
| Ple19I | CGATCG | 1 cut(s) | 66 |
| PmaCI | CACGTG | 1 cut(s) | 365 |
| PmlI | CACGTG | 1 cut(s) | 365 |
| Ppu21I | YACGTR | 1 cut(s) | 365 |
| PshAI | GACNNNNGTC | 1 cut(s) | 113 |
| Psp6I | CCWGG | 2 cut(s) | 273, 294 |
| PspCI | CACGTG | 1 cut(s) | 365 |
| PspFI | CCCAGC | 2 cut(s) | 390, 403 |
| PspGI | CCWGG | 2 cut(s) | 273, 294 |
| PspN4I | GGNNCC | 1 cut(s) | 193 |
| PspPI | GGNCC | 2 cut(s) | 271, 292 |
| PsuI | RGATCY | 1 cut(s) | 348 |
| PvuI | CGATCG | 1 cut(s) | 66 |
| PvuII | CAGCTG | 1 cut(s) | 407 |
| RsaI | GTAC | 3 cut(s) | 81, 284, 321 |
| RsaNI | GTAC | 3 cut(s) | 80, 283, 320 |
| SaqAI | TTAA | 1 cut(s) | 288 |
| SatI | GCNGC | 2 cut(s) | 44, 270 |
| Sau3AI | GATC | 5 cut(s) | 63, 102, 121, 206, 348 |
| Sau96I | GGNCC | 2 cut(s) | 271, 292 |
| ScrFI | CCNGG | 3 cut(s) | 53, 275, 296 |
| SetI | ASST | 9 cut(s) | 48, 75, 117, 225, 262, 267, 367, 382, 409 |
| SfaNI | GCATC | 2 cut(s) | 79, 345 |
| SsiI | CCGC | 2 cut(s) | 173, 269 |
| StyD4I | CCNGG | 3 cut(s) | 51, 273, 294 |
| TaiI | ACGT | 2 cut(s) | 75, 367 |
| TaqI | TCGA | 2 cut(s) | 66, 157 |
| TatI | WGTACW | 2 cut(s) | 282, 319 |
| TauI | GCSGC | 1 cut(s) | 272 |
| Tru1I | TTAA | 1 cut(s) | 288 |
| Tru9I | TTAA | 1 cut(s) | 288 |
| TscAI | CASTG | 1 cut(s) | 22 |
| TseFI | GTSAC | 2 cut(s) | 106, 244 |
| TseI | GCWGC | 1 cut(s) | 43 |
| Tsp45I | GTSAC | 2 cut(s) | 106, 244 |
| TspRI | CASTG | 1 cut(s) | 22 |
| ZraI | GACGTC | 1 cut(s) | 73 |