Plant transposase (Ptta/En/Spm family)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 2 cut(s) | 223, 384 |
| Acc16I | TGCGCA | 1 cut(s) | 5 |
| AccII | CGCG | 1 cut(s) | 484 |
| AciI | CCGC | 1 cut(s) | 484 |
| AclWI | GGATC | 2 cut(s) | 20, 438 |
| AcoI | YGGCCR | 1 cut(s) | 74 |
| AcsI | RAATTY | 1 cut(s) | 119 |
| AcyI | GRCGYC | 2 cut(s) | 220, 381 |
| AfaI | GTAC | 1 cut(s) | 440 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 79, 376 |
| AflIII | ACRYGT | 1 cut(s) | 249 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 219 |
| AjiI | CACGTC | 1 cut(s) | 250 |
| AjnI | CCWGG | 2 cut(s) | 274, 426 |
| AluBI | AGCT | 2 cut(s) | 152, 298 |
| AluI | AGCT | 2 cut(s) | 152, 298 |
| Alw26I | GTCTC | 2 cut(s) | 206, 488 |
| AlwI | GGATC | 2 cut(s) | 20, 438 |
| Ama87I | CYCGRG | 2 cut(s) | 153, 392 |
| AoxI | GGCC | 1 cut(s) | 74 |
| ApeKI | GCWGC | 1 cut(s) | 295 |
| ApoI | RAATTY | 1 cut(s) | 119 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 104, 136, 427, 459 |
| AspLEI | GCGC | 2 cut(s) | 6, 484 |
| AspS9I | GGNCC | 2 cut(s) | 70, 424 |
| AsuII | TTCGAA | 1 cut(s) | 188 |
| AvaI | CYCGRG | 2 cut(s) | 153, 392 |
| AvaII | GGWCC | 2 cut(s) | 70, 424 |
| BbvI | GCAGC | 1 cut(s) | 307 |
| BccI | CCATC | 2 cut(s) | 64, 367 |
| BceAI | ACGGC | 1 cut(s) | 513 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 268, 302 |
| BciT130I | CCWGG | 2 cut(s) | 276, 428 |
| BcoDI | GTCTC | 2 cut(s) | 206, 488 |
| BfaI | CTAG | 3 cut(s) | 44, 239, 514 |
| BisI | GCNGC | 1 cut(s) | 296 |
| BlpI | GCTNAGC | 1 cut(s) | 291 |
| BlsI | GCNGC | 1 cut(s) | 297 |
| Bme1390I | CCNGG | 2 cut(s) | 276, 428 |
| Bme18I | GGWCC | 2 cut(s) | 70, 424 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 219 |
| BmeT110I | CYCGRG | 2 cut(s) | 153, 392 |
| BmgBI | CACGTC | 1 cut(s) | 250 |
| BmgT120I | GGNCC | 2 cut(s) | 70, 424 |
| BmrFI | CCNGG | 2 cut(s) | 276, 428 |
| BmsI | GCATC | 1 cut(s) | 316 |
| BoxI | GACNNNNGTC | 1 cut(s) | 422 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 291 |
| Bpu14I | TTCGAA | 1 cut(s) | 188 |
| BpuEI | CTTGAG | 1 cut(s) | 230 |
| Bsa29I | ATCGAT | 1 cut(s) | 24 |
| BsaHI | GRCGYC | 2 cut(s) | 220, 381 |
| BsaJI | CCNNGG | 2 cut(s) | 360, 393 |
| BsaWI | WCCGGW | 1 cut(s) | 182 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 79, 376 |
| Bse118I | RCCGGY | 1 cut(s) | 72 |
| BseBI | CCWGG | 2 cut(s) | 276, 428 |
| BseCI | ATCGAT | 1 cut(s) | 24 |
| BseDI | CCNNGG | 2 cut(s) | 360, 393 |
| BseGI | GGATG | 2 cut(s) | 56, 403 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 79, 376 |
| BseMII | CTCAG | 2 cut(s) | 282, 411 |
| BseXI | GCAGC | 1 cut(s) | 307 |
| Bsh1236I | CGCG | 1 cut(s) | 484 |
| Bsh1285I | CGRYCG | 1 cut(s) | 445 |
| BshFI | GGCC | 1 cut(s) | 76 |
| BshVI | ATCGAT | 1 cut(s) | 24 |
| BsiEI | CGRYCG | 1 cut(s) | 445 |
| BsiHKCI | CYCGRG | 2 cut(s) | 153, 392 |
| BsiSI | CCGG | 2 cut(s) | 73, 183 |
| BslFI | GGGAC | 1 cut(s) | 272 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 79, 376 |
| BsmAI | GTCTC | 2 cut(s) | 206, 488 |
| BsmBI | CGTCTC | 1 cut(s) | 488 |
| BsmFI | GGGAC | 1 cut(s) | 272 |
| BsnI | GGCC | 1 cut(s) | 76 |
| BsoBI | CYCGRG | 2 cut(s) | 153, 392 |
| Bsp119I | TTCGAA | 1 cut(s) | 188 |
| Bsp143I | GATC | 5 cut(s) | 25, 243, 285, 411, 430 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 291 |
| BspACI | CCGC | 1 cut(s) | 484 |
| BspANI | GGCC | 1 cut(s) | 76 |
| BspCNI | CTCAG | 2 cut(s) | 283, 412 |
| BspDI | ATCGAT | 1 cut(s) | 24 |
| BspFNI | CGCG | 1 cut(s) | 484 |
| BspHI | TCATGA | 1 cut(s) | 414 |
| BspPI | GGATC | 2 cut(s) | 20, 438 |
| BspT104I | TTCGAA | 1 cut(s) | 188 |
| BsrFI | RCCGGY | 1 cut(s) | 72 |
| BssAI | RCCGGY | 1 cut(s) | 72 |
| BssECI | CCNNGG | 2 cut(s) | 360, 393 |
| BssMI | GATC | 5 cut(s) | 25, 243, 285, 411, 430 |
| BssNI | GRCGYC | 2 cut(s) | 220, 381 |
| Bst2UI | CCWGG | 2 cut(s) | 276, 428 |
| Bst4CI | ACNGT | 1 cut(s) | 443 |
| Bst6I | CTCTTC | 1 cut(s) | 512 |
| BstACI | GRCGYC | 2 cut(s) | 220, 381 |
| BstBI | TTCGAA | 1 cut(s) | 188 |
| BstC8I | GCNNGC | 2 cut(s) | 142, 502 |
| BstDEI | CTNAG | 4 cut(s) | 162, 231, 291, 420 |
| BstF5I | GGATG | 2 cut(s) | 56, 403 |
| BstFNI | CGCG | 1 cut(s) | 484 |
| BstHHI | GCGC | 2 cut(s) | 6, 484 |
| BstKTI | GATC | 5 cut(s) | 28, 246, 288, 414, 433 |
| BstMAI | GTCTC | 2 cut(s) | 206, 488 |
| BstMBI | GATC | 5 cut(s) | 25, 243, 285, 411, 430 |
| BstMCI | CGRYCG | 1 cut(s) | 445 |
| BstNI | CCWGG | 2 cut(s) | 276, 428 |
| BstNSI | RCATGY | 1 cut(s) | 504 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 422 |
| BstSCI | CCNGG | 2 cut(s) | 274, 426 |
| BstUI | CGCG | 1 cut(s) | 484 |
| BstV1I | GCAGC | 1 cut(s) | 307 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 84 |
| Bsu15I | ATCGAT | 1 cut(s) | 24 |
| BsuRI | GGCC | 1 cut(s) | 76 |
| BsuTUI | ATCGAT | 1 cut(s) | 24 |
| BtgZI | GCGATG | 2 cut(s) | 333, 339 |
| BtrI | CACGTC | 1 cut(s) | 250 |
| BtsCI | GGATG | 2 cut(s) | 56, 403 |
| BtsI | GCAGTG | 1 cut(s) | 286 |
| BtsIMutI | CAGTG | 1 cut(s) | 286 |
| Cac8I | GCNNGC | 2 cut(s) | 142, 502 |
| CciI | TCATGA | 1 cut(s) | 414 |
| CfoI | GCGC | 2 cut(s) | 6, 484 |
| Cfr10I | RCCGGY | 1 cut(s) | 72 |
| Cfr13I | GGNCC | 2 cut(s) | 70, 424 |
| ClaI | ATCGAT | 1 cut(s) | 24 |
| Csp6I | GTAC | 1 cut(s) | 439 |
| CviAII | CATG | 4 cut(s) | 302, 415, 452, 501 |
| CviJI | RGCY | 6 cut(s) | 76, 94, 152, 193, 208, 298 |
| CviKI_1 | RGCY | 6 cut(s) | 76, 94, 152, 193, 208, 298 |
| CviQI | GTAC | 1 cut(s) | 439 |
| DdeI | CTNAG | 4 cut(s) | 162, 231, 291, 420 |
| DpnI | GATC | 5 cut(s) | 27, 245, 287, 413, 432 |
| DpnII | GATC | 5 cut(s) | 25, 243, 285, 411, 430 |
| DriI | GACNNNNNGTC | 1 cut(s) | 219 |
| EaeI | YGGCCR | 1 cut(s) | 74 |
| Eam1104I | CTCTTC | 1 cut(s) | 512 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 219 |
| EarI | CTCTTC | 1 cut(s) | 512 |
| Eco47I | GGWCC | 2 cut(s) | 70, 424 |
| Eco88I | CYCGRG | 2 cut(s) | 153, 392 |
| EcoO109I | RGGNCCY | 1 cut(s) | 424 |
| EcoRII | CCWGG | 2 cut(s) | 274, 426 |
| Esp3I | CGTCTC | 1 cut(s) | 488 |
| FaeI | CATG | 4 cut(s) | 305, 418, 455, 504 |
| FaiI | YATR | 7 cut(s) | 204, 303, 311, 416, 453, 473, 502 |
| FaqI | GGGAC | 1 cut(s) | 272 |
| FatI | CATG | 4 cut(s) | 301, 414, 451, 500 |
| FauI | CCCGC | 1 cut(s) | 477 |
| Fnu4HI | GCNGC | 1 cut(s) | 296 |
| FokI | GGATG | 2 cut(s) | 43, 410 |
| Fsp4HI | GCNGC | 1 cut(s) | 296 |
| FspAI | RTGCGCAY | 1 cut(s) | 5 |
| FspBI | CTAG | 3 cut(s) | 44, 239, 514 |
| FspI | TGCGCA | 1 cut(s) | 5 |
| GlaI | GCGC | 2 cut(s) | 5, 483 |
| GluI | GCNGC | 1 cut(s) | 296 |
| HaeIII | GGCC | 1 cut(s) | 76 |
| HapII | CCGG | 2 cut(s) | 73, 183 |
| HhaI | GCGC | 2 cut(s) | 6, 484 |
| Hin1I | GRCGYC | 2 cut(s) | 220, 381 |
| Hin1II | CATG | 4 cut(s) | 305, 418, 455, 504 |
| Hin6I | GCGC | 2 cut(s) | 4, 482 |
| HinP1I | GCGC | 2 cut(s) | 4, 482 |
| HinfI | GANTC | 1 cut(s) | 158 |
| HpaII | CCGG | 2 cut(s) | 73, 183 |
| Hpy166II | GTNNAC | 1 cut(s) | 265 |
| Hpy188I | TCNGA | 2 cut(s) | 364, 411 |
| Hpy188III | TCNNGA | 5 cut(s) | 29, 44, 155, 415, 491 |
| Hpy8I | GTNNAC | 1 cut(s) | 265 |
| Hpy99I | CGWCG | 3 cut(s) | 222, 251, 450 |
| HpyAV | CCTTC | 1 cut(s) | 472 |
| HpyCH4III | ACNGT | 1 cut(s) | 443 |
| HpyCH4IV | ACGT | 5 cut(s) | 220, 249, 261, 267, 381 |
| HpyCH4V | TGCA | 1 cut(s) | 99 |
| HpyF3I | CTNAG | 4 cut(s) | 162, 231, 291, 420 |
| HpySE526I | ACGT | 5 cut(s) | 220, 249, 261, 267, 381 |
| Hsp92I | GRCGYC | 2 cut(s) | 220, 381 |
| Hsp92II | CATG | 4 cut(s) | 305, 418, 455, 504 |
| HspAI | GCGC | 2 cut(s) | 4, 482 |
| Kzo9I | GATC | 5 cut(s) | 25, 243, 285, 411, 430 |
| Lsp1109I | GCAGC | 1 cut(s) | 307 |
| LweI | GCATC | 1 cut(s) | 316 |
| MaeI | CTAG | 3 cut(s) | 44, 239, 514 |
| MaeII | ACGT | 5 cut(s) | 220, 249, 261, 267, 381 |
| MaeIII | GTNAC | 1 cut(s) | 382 |
| MalI | GATC | 5 cut(s) | 27, 245, 287, 413, 432 |
| MboI | GATC | 5 cut(s) | 25, 243, 285, 411, 430 |
| MboII | GAAGA | 3 cut(s) | 139, 499, 502 |
| MluCI | AATT | 1 cut(s) | 119 |
| MlyI | GAGTC | 1 cut(s) | 167 |
| MmeI | TCCRAC | 1 cut(s) | 45 |
| MnlI | CCTC | 9 cut(s) | 25, 40, 61, 73, 370, 370, 388, 415, 515 |
| MseI | TTAA | 2 cut(s) | 11, 20 |
| MspI | CCGG | 2 cut(s) | 73, 183 |
| MspR9I | CCNGG | 2 cut(s) | 276, 428 |
| MvaI | CCWGG | 2 cut(s) | 276, 428 |
| MvnI | CGCG | 1 cut(s) | 484 |
| NdeII | GATC | 5 cut(s) | 25, 243, 285, 411, 430 |
| NlaIII | CATG | 4 cut(s) | 305, 418, 455, 504 |
| NmuCI | GTSAC | 1 cut(s) | 382 |
| NsbI | TGCGCA | 1 cut(s) | 5 |
| NspI | RCATGY | 1 cut(s) | 504 |
| NspV | TTCGAA | 1 cut(s) | 188 |
| PaeI | GCATGC | 1 cut(s) | 504 |
| PagI | TCATGA | 1 cut(s) | 414 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 368 |
| PfoI | TCCNGGA | 1 cut(s) | 426 |
| PkrI | GCNGC | 1 cut(s) | 297 |
| PleI | GAGTC | 1 cut(s) | 166 |
| PpsI | GAGTC | 1 cut(s) | 166 |
| PpuMI | RGGWCCY | 1 cut(s) | 424 |
| PshAI | GACNNNNGTC | 1 cut(s) | 422 |
| Psp5II | RGGWCCY | 1 cut(s) | 424 |
| Psp6I | CCWGG | 2 cut(s) | 274, 426 |
| PspGI | CCWGG | 2 cut(s) | 274, 426 |
| PspPI | GGNCC | 2 cut(s) | 70, 424 |
| PspPPI | RGGWCCY | 1 cut(s) | 424 |
| RsaI | GTAC | 1 cut(s) | 440 |
| RsaNI | GTAC | 1 cut(s) | 439 |
| SaqAI | TTAA | 2 cut(s) | 11, 20 |
| SatI | GCNGC | 1 cut(s) | 296 |
| Sau3AI | GATC | 5 cut(s) | 25, 243, 285, 411, 430 |
| Sau96I | GGNCC | 2 cut(s) | 70, 424 |
| SchI | GAGTC | 1 cut(s) | 167 |
| ScrFI | CCNGG | 2 cut(s) | 276, 428 |
| SfaNI | GCATC | 1 cut(s) | 316 |
| SfuI | TTCGAA | 1 cut(s) | 188 |
| SinI | GGWCC | 2 cut(s) | 70, 424 |
| SmlI | CTYRAG | 1 cut(s) | 209 |
| SmoI | CTYRAG | 1 cut(s) | 209 |
| SphI | GCATGC | 1 cut(s) | 504 |
| Sse9I | AATT | 1 cut(s) | 119 |
| SsiI | CCGC | 1 cut(s) | 484 |
| SspMI | CTAG | 3 cut(s) | 44, 239, 514 |
| StyD4I | CCNGG | 2 cut(s) | 274, 426 |
| TaaI | ACNGT | 1 cut(s) | 443 |
| TaiI | ACGT | 5 cut(s) | 223, 252, 264, 270, 384 |
| TaqI | TCGA | 4 cut(s) | 24, 188, 246, 271 |
| TasI | AATT | 1 cut(s) | 119 |
| Tru1I | TTAA | 2 cut(s) | 11, 20 |
| Tru9I | TTAA | 2 cut(s) | 11, 20 |
| TscAI | CASTG | 1 cut(s) | 286 |
| TseFI | GTSAC | 1 cut(s) | 382 |
| TseI | GCWGC | 1 cut(s) | 295 |
| Tsp45I | GTSAC | 1 cut(s) | 382 |
| TspDTI | ATGAA | 1 cut(s) | 290 |
| TspRI | CASTG | 1 cut(s) | 286 |
| VpaK11BI | GGWCC | 2 cut(s) | 70, 424 |
| XapI | RAATTY | 1 cut(s) | 119 |
| XbaI | TCTAGA | 1 cut(s) | 43 |
| XceI | RCATGY | 1 cut(s) | 504 |
| XspI | CTAG | 3 cut(s) | 44, 239, 514 |
| ZraI | GACGTC | 2 cut(s) | 221, 382 |