Rh2DG020300

Plant transposase (Ptta/En/Spm family)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000260
Physical Location & Seq
Forward (+)
1209062 .. 1212156
3095 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG020300.1

Sequence Viewer

Length: 1524 bp
ATGGGGATAGACGATTATTACTATGGTAGCCCAAAAAATCCGAGCAGGGAAGCCGACCCGAGTTCCACACGACTCAGATCGTCGTCGTCGTCGTCGTCGTTAAATTTCAGGGCTCAATTCCCGCCCTTAATTTTGTTTCAGGGCTCCAAAGGTTCAATCTTTATTGTAAAATGTGCGTCCAATACGTACTGGGTTAATGGGTTTCAATTAATTTTGGGTTCCCCAGTAAAATTCCGTCCTCTCAAAACCATGGAACACGAAGAGGGCCCCACCGTCACCGCCATCGTCCCCTTCATCCCCACCGCCGCCGCCTCCGCCATTGTCGCAGTCAGCGCCGCAGAATCCGCCGTCGTCACAGTCGCAGCCAACCCACCGTGTCCAACCAACGACTCGGACCCCGATCAATCGGCGGTGGCCGGCGGCGGCGGTCTCTCCGAGGATCTTAAACAAAAGCAGATAGATGTGGTTGCGGGTAAGAAGAGGCCCCGTAAGGAGACTAAGTGCACGGCGGCGGGTAAGAAGAGACCCCGGAATGGGACAAAGTCCATGACTGCTGAGACTGCTGACATTAGTACTCAGCACATTGTTCATCAGAGGAGGGCTCGCGGAACTCAAGCCGAAAATGGAGAGGTCACAGAAGTGACTGCATCTTCAACTGGTACTGGTTCAACACAATCAGATGATGTAGTGCCAATAACAAAAGAAAGCAAAAAGAGGAAGAATGGGCCTGGAAAGCGAGGACCATCCACAGGCTCTAAAGCTGAGCAAATTAGGAGAAGAACAGGGAATAGGATTTCTGTTAATCTTGATCCTGAGCATGGAAGGCCGACTGATAAAGGCGTTTCAAGCCTTTTGTCTAATGAGTGTGGGAAGATTGTCAGGGAGAAGTGCCCAATGGTGCATGAAACGTGGACAAAGATTTCCAGGGACGTGAAACATAACCTCAGAAAGGAGGTTGAAGTGAACTTTGAAGTTGATGTACAGCGCCCATCAATTATACATTACTTGAATAAGGTTATGGGTAAGGTGTACAGGGAGTTTAAGTGTGAGTTGCACAAGCACTACAAAAAGATGAGGTCCCTTGAAGAGGCACTTGATAAACCCCCCAAGGCAATGATAGCTAGAAACGATCCAGATCAGTGGAAGTGTTTGTGTAAACATTTCTCAGAGGAAAAGTTTAAGAGGAAAAGTGAAACCAATGCAAAAAATCGAGCTGGAAAGGAGTATGAGCACTGTGGAGGTTCTCGTCCTCATGGTTTATATTTTCAAGATAAAGTTCAGGAGGGTTCAAAGTTTCCCGCAATCGAGACCTTCAGAGTGACTCATCTCAAAAAGGGGAGTAATGAATGGATCAGTAACAAAGCTCAGGAACAATATGTATGTTTGAAATTTCATTACTCATTTATCGTATTACACGTTATAGATGAAAAGTCAGCAAATGTGAATGTCTTCATATCAAACATTATTGATATTTCAGCCTTAACAAAACTTTATTGTGTCTGCTTGTTTAAACATGTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transposase_24

Protein Analysis

507

Amino Acids

56.32

Weight (kDa)

9.47

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_24 PF03004 377 - 459 3.9e-10 Plant transposase (Ptta/En/Spm family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000260)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01701 FvH4_4g03270
malus_domestica MD00G1017800.v1.1 MD00G1142300.v1.1 MD00G1145500.v1.1 MD00G1191000.v1.1 MD01G1112800.v1.1 MD01G1143700.v1.1 MD02G1048600.v1.1 MD02G1196000.v1.1 MD03G1062700.v1.1 MD03G1095500.v1.1 MD03G1095600.v1.1 MD03G1125500.v1.1 MD03G1154200.v1.1 MD03G1154300.v1.1 MD03G1170400.v1.1 MD03G1179800.v1.1 MD03G1196800.v1.1 MD03G1255700.v1.1 MD04G1029900.v1.1 MD04G1054700.v1.1 MD04G1102500.v1.1 MD04G1142700.v1.1 MD05G1052900.v1.1 MD05G1063000.v1.1 MD05G1069200.v1.1 MD05G1160700.v1.1 MD05G1235500.v1.1 MD05G1285100.v1.1 MD05G1321300.v1.1 MD07G1170900.v1.1 MD08G1072400.v1.1 MD08G1151000.v1.1 MD09G1075100.v1.1 MD11G1004200.v1.1 MD11G1134000.v1.1 MD11G1134100.v1.1 MD11G1150600.v1.1 MD11G1167200.v1.1 MD11G1169500.v1.1 MD11G1191200.v1.1 MD11G1215700.v1.1 MD12G1110400.v1.1 MD12G1226000.v1.1 MD14G1070600.v1.1 MD15G1246900.v1.1 MD15G1271300.v1.1 MD15G1304400.v1.1 MD15G1306500.v1.1 MD15G1394800.v1.1 MD16G1222400.v1.1 MD16G1235600.v1.1 MD16G1275200.v1.1 MD17G1164900.v1.1 MD17G1199400.v1.1 MD17G1200600.v1.1 MD17G1255500.v1.1
prunus_persica Prupe.5G199500_v2.0.a1
pyrus_communis pycom01g05550 pycom01g06510 pycom01g10640 pycom02g04780 pycom02g10380 pycom02g16940 pycom02g21170 pycom02g25250 pycom03g11350 pycom03g15980 pycom03g17500 pycom04g07430 pycom04g11390 pycom05g02890 pycom05g12620 pycom05g21240 pycom06g01740 pycom06g06680 pycom06g08420 pycom07g09890 pycom07g10330 pycom07g13390 pycom07g13400 pycom07g14390 pycom08g04050 pycom08g04060 pycom08g09610 pycom08g14690 pycom09g14670 pycom09g14680 pycom09g19450 pycom10g09940 pycom10g14290 pycom11g03600 pycom11g13710 pycom11g14810 pycom11g17720 pycom12g02240 pycom12g06830 pycom12g09950 pycom12g13060 pycom13g02180 pycom13g07740 pycom13g21900 pycom13g23040 pycom14g10400 pycom14g10410 pycom14g10490 pycom14g10680 pycom14g13340 pycom15g15950 pycom15g31530 pycom15g31550 pycom15g38660 pycom16g08600 pycom16g20570 pycom17g09630 pycom17g09650 pycom17g16800 pycom17g16830 pycom17g24070
rosa_chinensis RchiOBHm_Chr2g0086631
rosa_laevigata RLG00000015768
rosa_multiflora Rmu_co8061662.1_g000001 Rmu_co8118782.1_g000001
rosa_roxburghii Rroxscaffold_2G00154370
rosa_rugosa Rorug01G0468000
rosa_samantha Rh2AG019800 Rh2BG019700 Rh2BG019800 Rh2CG019500 Rh2DG020300
rosa_wichuraiana Rw2G001640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 606
AclWI GGATC 4 cut(s) 447, 803, 1124, 1358
AcoI YGGCCR 1 cut(s) 414
AcsI RAATTY 3 cut(s) 103, 230, 1388
AcuI CTGAAG 1 cut(s) 1297
AfaI GTAC 5 cut(s) 188, 574, 661, 981, 1031
AfiI CCNNNNNNNGG 6 cut(s) 533, 534, 749, 818, 949, 1087
AflIII ACRYGT 2 cut(s) 1414, 1513
AjiI CACGTC 1 cut(s) 931
AjnI CCWGG 2 cut(s) 727, 923
AleI CACNNNNGTG 1 cut(s) 638
AluBI AGCT 4 cut(s) 761, 1121, 1214, 1364
AluI AGCT 4 cut(s) 761, 1121, 1214, 1364
Alw21I GWGCWC 2 cut(s) 506, 1233
Alw26I GTCTC 5 cut(s) 434, 488, 517, 551, 1301
Alw44I GTGCAC 1 cut(s) 502
AlwI GGATC 4 cut(s) 447, 803, 1124, 1358
Ama87I CYCGRG 1 cut(s) 58
AoxI GGCC 5 cut(s) 265, 414, 482, 725, 824
ApaI GGGCCC 1 cut(s) 269
ApaLI GTGCAC 1 cut(s) 502
ApeKI GCWGC 1 cut(s) 362
ApoI RAATTY 3 cut(s) 103, 230, 1388
AseI ATTAAT 1 cut(s) 209
Asp700I GAANNNNTTC 1 cut(s) 1448
AspLEI GCGC 2 cut(s) 335, 987
AspS9I GGNCC 7 cut(s) 265, 266, 394, 483, 725, 740, 1077
AsuC2I CCSGG 1 cut(s) 529
AsuHPI GGTGA 1 cut(s) 268
AvaI CYCGRG 1 cut(s) 58
AvaII GGWCC 3 cut(s) 394, 740, 1077
BaeGI GKGCMC 3 cut(s) 269, 506, 893
BanII GRGCYC 4 cut(s) 115, 146, 269, 604
BarI GAAGNNNNNNTAC 2 cut(s) 963, 995
BbsI GAAGAC 1 cut(s) 1441
Bbv12I GWGCWC 2 cut(s) 506, 1233
BbvI GCAGC 1 cut(s) 374
BccI CCATC 3 cut(s) 290, 751, 997
BceAI ACGGC 2 cut(s) 332, 522
BciT130I CCWGG 2 cut(s) 729, 925
BcnI CCSGG 1 cut(s) 529
BcoDI GTCTC 5 cut(s) 434, 488, 517, 551, 1301
BfaI CTAG 1 cut(s) 1122
BfoI RGCGCY 2 cut(s) 336, 988
BisI GCNGC 7 cut(s) 306, 309, 336, 363, 421, 424, 510
BlpI GCTNAGC 1 cut(s) 762
BlsI GCNGC 7 cut(s) 307, 310, 337, 364, 422, 425, 511
BmcAI AGTACT 1 cut(s) 574
Bme1390I CCNGG 3 cut(s) 529, 729, 925
Bme18I GGWCC 3 cut(s) 394, 740, 1077
BmeT110I CYCGRG 1 cut(s) 58
BmgBI CACGTC 1 cut(s) 931
BmgT120I GGNCC 7 cut(s) 265, 266, 394, 483, 725, 740, 1077
BmiI GGNNCC 7 cut(s) 145, 220, 267, 268, 396, 485, 1079
BmrFI CCNGG 3 cut(s) 529, 729, 925
BmrI ACTGGG 2 cut(s) 199, 218
BmsI GCATC 1 cut(s) 656
BmuI ACTGGG 2 cut(s) 199, 218
BpiI GAAGAC 1 cut(s) 1441
BplI GAGNNNNNCTC 2 cut(s) 586, 618
Bpu10I CCTNAGC 2 cut(s) 813, 1365
Bpu1102I GCTNAGC 1 cut(s) 762
BpuEI CTTGAG 1 cut(s) 597
BpuMI CCSGG 1 cut(s) 529
BsaAI YACGTR 1 cut(s) 186
BsaBI GATNNNNATC 1 cut(s) 1134
BsaI GGTCTC 3 cut(s) 434, 517, 1301
BsaJI CCNNGG 5 cut(s) 249, 435, 527, 924, 1107
BsaXI ACNNNNNCTCC 4 cut(s) 485, 515, 1028, 1058
Bsc4I CCNNNNNNNGG 6 cut(s) 533, 534, 749, 818, 949, 1087
Bse118I RCCGGY 1 cut(s) 416
Bse1I ACTGG 4 cut(s) 194, 224, 661, 667
Bse3DI GCAATG 1 cut(s) 1119
Bse8I GATNNNNATC 1 cut(s) 1134
BseBI CCWGG 2 cut(s) 729, 925
BseDI CCNNGG 5 cut(s) 249, 435, 527, 924, 1107
BseGI GGATG 2 cut(s) 294, 743
BseJI GATNNNNATC 1 cut(s) 1134
BseLI CCNNNNNNNGG 6 cut(s) 533, 534, 749, 818, 949, 1087
BseMI GCAATG 1 cut(s) 1119
BseMII CTCAG 8 cut(s) 88, 546, 590, 753, 804, 958, 1179, 1379
BseNI ACTGG 4 cut(s) 194, 224, 661, 667
BseRI GAGGAG 1 cut(s) 610
BseSI GKGCMC 3 cut(s) 269, 506, 893
BseXI GCAGC 1 cut(s) 374
Bsh1236I CGCG 1 cut(s) 606
BshFI GGCC 5 cut(s) 267, 416, 484, 727, 826
BsiHKAI GWGCWC 2 cut(s) 506, 1233
BsiHKCI CYCGRG 1 cut(s) 58
BsiSI CCGG 2 cut(s) 417, 529
BslFI GGGAC 4 cut(s) 272, 550, 941, 1063
BslI CCNNNNNNNGG 6 cut(s) 533, 534, 749, 818, 949, 1087
BsmAI GTCTC 5 cut(s) 434, 488, 517, 551, 1301
BsmFI GGGAC 4 cut(s) 272, 550, 941, 1063
BsnI GGCC 5 cut(s) 267, 416, 484, 727, 826
Bso31I GGTCTC 3 cut(s) 434, 517, 1301
BsoBI CYCGRG 1 cut(s) 58
Bsp120I GGGCCC 1 cut(s) 265
Bsp1286I GDGCHC 7 cut(s) 115, 146, 269, 506, 604, 893, 1233
Bsp1407I TGTACA 2 cut(s) 979, 1029
Bsp143I GATC 7 cut(s) 77, 400, 439, 808, 1129, 1135, 1350
Bsp1720I GCTNAGC 1 cut(s) 762
Bsp19I CCATGG 1 cut(s) 249
BspANI GGCC 5 cut(s) 267, 416, 484, 727, 826
BspCNI CTCAG 8 cut(s) 87, 547, 589, 754, 805, 957, 1178, 1378
BspFNI CGCG 1 cut(s) 606
BspLI GGNNCC 7 cut(s) 145, 220, 267, 268, 396, 485, 1079
BspPI GGATC 4 cut(s) 447, 803, 1124, 1358
BspTNI GGTCTC 3 cut(s) 434, 517, 1301
BsrDI GCAATG 1 cut(s) 1119
BsrFI RCCGGY 1 cut(s) 416
BsrGI TGTACA 2 cut(s) 979, 1029
BsrI ACTGG 4 cut(s) 194, 224, 661, 667
BssAI RCCGGY 1 cut(s) 416
BssECI CCNNGG 5 cut(s) 249, 435, 527, 924, 1107
BssMI GATC 7 cut(s) 77, 400, 439, 808, 1129, 1135, 1350
BssT1I CCWWGG 2 cut(s) 249, 1107
Bst2UI CCWGG 2 cut(s) 729, 925
Bst4CI ACNGT 4 cut(s) 274, 358, 375, 1235
Bst6I CTCTTC 4 cut(s) 255, 473, 515, 1080
BstAUI TGTACA 2 cut(s) 979, 1029
BstBAI YACGTR 1 cut(s) 186
BstC8I GCNNGC 2 cut(s) 418, 604
BstDEI CTNAG 9 cut(s) 74, 498, 555, 576, 762, 813, 944, 1165, 1365
BstDSI CCRYGG 1 cut(s) 249
BstENI CCTNNNNNAGG 2 cut(s) 947, 1085
BstF5I GGATG 2 cut(s) 294, 743
BstFNI CGCG 1 cut(s) 606
BstH2I RGCGCY 2 cut(s) 336, 988
BstHHI GCGC 2 cut(s) 335, 987
BstKTI GATC 7 cut(s) 80, 403, 442, 811, 1132, 1138, 1353
BstMAI GTCTC 5 cut(s) 434, 488, 517, 551, 1301
BstMBI GATC 7 cut(s) 77, 400, 439, 808, 1129, 1135, 1350
BstMWI GCNNNNNNNGC 9 cut(s) 314, 323, 332, 344, 560, 733, 823, 846, 1118
BstNI CCWGG 2 cut(s) 729, 925
BstNSI RCATGY 1 cut(s) 1517
BstSCI CCNGG 3 cut(s) 527, 727, 923
BstSLI GKGCMC 3 cut(s) 269, 506, 893
BstSNI TACGTA 1 cut(s) 186
BstUI CGCG 1 cut(s) 606
BstV1I GCAGC 1 cut(s) 374
BstV2I GAAGAC 1 cut(s) 1441
BstX2I RGATCY 1 cut(s) 439
BstXI CCANNNNNNTGG 1 cut(s) 1140
BstYI RGATCY 1 cut(s) 439
BsuRI GGCC 5 cut(s) 267, 416, 484, 727, 826
BtgI CCRYGG 1 cut(s) 249
BtrI CACGTC 1 cut(s) 931
BtsCI GGATG 2 cut(s) 294, 743
BtsIMutI CAGTG 2 cut(s) 1145, 1231
Cac8I GCNNGC 2 cut(s) 418, 604
CfoI GCGC 2 cut(s) 335, 987
Cfr10I RCCGGY 1 cut(s) 416
Cfr13I GGNCC 7 cut(s) 265, 266, 394, 483, 725, 740, 1077
CseI GACGC 1 cut(s) 165
Csp6I GTAC 5 cut(s) 187, 573, 660, 980, 1030
CviAII CATG 6 cut(s) 250, 547, 818, 902, 1253, 1514
CviQI GTAC 5 cut(s) 187, 573, 660, 980, 1030
DdeI CTNAG 9 cut(s) 74, 498, 555, 576, 762, 813, 944, 1165, 1365
DpnI GATC 7 cut(s) 79, 402, 441, 810, 1131, 1137, 1352
DpnII GATC 7 cut(s) 77, 400, 439, 808, 1129, 1135, 1350
DraI TTTAAA 1 cut(s) 1510
EaeI YGGCCR 1 cut(s) 414
Eam1104I CTCTTC 4 cut(s) 255, 473, 515, 1080
EarI CTCTTC 4 cut(s) 255, 473, 515, 1080
EciI GGCGGA 2 cut(s) 304, 334
Eco105I TACGTA 1 cut(s) 186
Eco130I CCWWGG 2 cut(s) 249, 1107
Eco24I GRGCYC 4 cut(s) 115, 146, 269, 604
Eco31I GGTCTC 3 cut(s) 434, 517, 1301
Eco47I GGWCC 3 cut(s) 394, 740, 1077
Eco57I CTGAAG 1 cut(s) 1297
Eco88I CYCGRG 1 cut(s) 58
EcoNI CCTNNNNNAGG 2 cut(s) 947, 1085
EcoO109I RGGNCCY 4 cut(s) 265, 266, 483, 1077
EcoRII CCWGG 2 cut(s) 727, 923
EcoT14I CCWWGG 2 cut(s) 249, 1107
EcoT38I GRGCYC 4 cut(s) 115, 146, 269, 604
ErhI CCWWGG 2 cut(s) 249, 1107
FaeI CATG 6 cut(s) 253, 550, 821, 905, 1256, 1517
FalI AAGNNNNNCTT 2 cut(s) 1077, 1109
FaqI GGGAC 4 cut(s) 272, 550, 941, 1063
FatI CATG 6 cut(s) 249, 546, 817, 901, 1252, 1513
FauI CCCGC 4 cut(s) 129, 463, 505, 1306
Fnu4HI GCNGC 7 cut(s) 306, 309, 336, 363, 421, 424, 510
FokI GGATG 2 cut(s) 281, 730
FriOI GRGCYC 4 cut(s) 115, 146, 269, 604
Fsp4HI GCNGC 7 cut(s) 306, 309, 336, 363, 421, 424, 510
FspBI CTAG 1 cut(s) 1122
GlaI GCGC 2 cut(s) 334, 986
GluI GCNGC 7 cut(s) 306, 309, 336, 363, 421, 424, 510
HaeII RGCGCY 2 cut(s) 336, 988
HaeIII GGCC 5 cut(s) 267, 416, 484, 727, 826
HapII CCGG 2 cut(s) 417, 529
HgaI GACGC 1 cut(s) 165
HhaI GCGC 2 cut(s) 335, 987
Hin1II CATG 6 cut(s) 253, 550, 821, 905, 1256, 1517
Hin6I GCGC 2 cut(s) 333, 985
HinP1I GCGC 2 cut(s) 333, 985
HinfI GANTC 4 cut(s) 72, 341, 389, 1321
HpaII CCGG 2 cut(s) 417, 529
HphI GGTGA 1 cut(s) 268
Hpy166II GTNNAC 5 cut(s) 504, 912, 964, 1030, 1157
Hpy188I TCNGA 9 cut(s) 42, 77, 394, 436, 594, 679, 947, 1168, 1316
Hpy188III TCNNGA 7 cut(s) 806, 812, 1133, 1268, 1280, 1306, 1367
Hpy8I GTNNAC 5 cut(s) 504, 912, 964, 1030, 1157
Hpy99I CGWCG 7 cut(s) 85, 88, 91, 94, 97, 100, 353
HpyAV CCTTC 3 cut(s) 301, 816, 1321
HpyCH4III ACNGT 4 cut(s) 274, 358, 375, 1235
HpyCH4IV ACGT 4 cut(s) 185, 908, 930, 1416
HpyCH4V TGCA 5 cut(s) 504, 647, 901, 1054, 1202
HpyF10VI GCNNNNNNNGC 9 cut(s) 314, 323, 332, 344, 560, 733, 823, 846, 1118
HpyF3I CTNAG 9 cut(s) 74, 498, 555, 576, 762, 813, 944, 1165, 1365
HpySE526I ACGT 4 cut(s) 185, 908, 930, 1416
Hsp92II CATG 6 cut(s) 253, 550, 821, 905, 1256, 1517
HspAI GCGC 2 cut(s) 333, 985
KroI GCCGGC 1 cut(s) 416
KroNI GCCGGC 1 cut(s) 418
Kzo9I GATC 7 cut(s) 77, 400, 439, 808, 1129, 1135, 1350
LmnI GCTCC 1 cut(s) 149
Lsp1109I GCAGC 1 cut(s) 374
LweI GCATC 1 cut(s) 656
MaeI CTAG 1 cut(s) 1122
MaeII ACGT 4 cut(s) 185, 908, 930, 1416
MaeIII GTNAC 6 cut(s) 274, 352, 631, 640, 1318, 1355
MalI GATC 7 cut(s) 79, 402, 441, 810, 1131, 1137, 1352
MboI GATC 7 cut(s) 77, 400, 439, 808, 1129, 1135, 1350
MboII GAAGA 9 cut(s) 272, 490, 532, 642, 730, 789, 883, 1097, 1441
MflI RGATCY 1 cut(s) 439
MhlI GDGCHC 7 cut(s) 115, 146, 269, 506, 604, 893, 1233
MluCI AATT 9 cut(s) 103, 116, 129, 206, 210, 230, 768, 993, 1388
MlyI GAGTC 3 cut(s) 66, 383, 1315
MmeI TCCRAC 1 cut(s) 404
MroNI GCCGGC 1 cut(s) 416
MroXI GAANNNNTTC 1 cut(s) 1448
MslI CAYNNNNRTG 1 cut(s) 638
MspI CCGG 2 cut(s) 417, 529
MspR9I CCNGG 3 cut(s) 529, 729, 925
MssI GTTTAAAC 1 cut(s) 1510
MvaI CCWGG 2 cut(s) 729, 925
MvnI CGCG 1 cut(s) 606
MwoI GCNNNNNNNGC 9 cut(s) 314, 323, 332, 344, 560, 733, 823, 846, 1118
NaeI GCCGGC 1 cut(s) 418
NciI CCSGG 1 cut(s) 529
NcoI CCATGG 1 cut(s) 249
NdeII GATC 7 cut(s) 77, 400, 439, 808, 1129, 1135, 1350
NgoMIV GCCGGC 1 cut(s) 416
NlaIII CATG 6 cut(s) 253, 550, 821, 905, 1256, 1517
NlaIV GGNNCC 7 cut(s) 145, 220, 267, 268, 396, 485, 1079
NmuCI GTSAC 5 cut(s) 274, 352, 631, 640, 1318
NspI RCATGY 1 cut(s) 1517
OliI CACNNNNGTG 1 cut(s) 638
PciI ACATGT 1 cut(s) 1513
PcsI WCGNNNNNNNCGW 5 cut(s) 86, 89, 92, 95, 1413
PdiI GCCGGC 1 cut(s) 418
PdmI GAANNNNTTC 1 cut(s) 1448
PfeI GAWTC 1 cut(s) 341
PflFI GACNNNGTC 1 cut(s) 541
PkrI GCNGC 7 cut(s) 307, 310, 337, 364, 422, 425, 511
PleI GAGTC 3 cut(s) 66, 383, 1315
PmeI GTTTAAAC 1 cut(s) 1510
PpsI GAGTC 3 cut(s) 66, 383, 1315
Ppu21I YACGTR 1 cut(s) 186
PpuMI RGGWCCY 1 cut(s) 1077
PscI ACATGT 1 cut(s) 1513
PshBI ATTAAT 1 cut(s) 209
Psp5II RGGWCCY 1 cut(s) 1077
Psp6I CCWGG 2 cut(s) 727, 923
PspGI CCWGG 2 cut(s) 727, 923
PspN4I GGNNCC 7 cut(s) 145, 220, 267, 268, 396, 485, 1079
PspOMI GGGCCC 1 cut(s) 265
PspPI GGNCC 7 cut(s) 265, 266, 394, 483, 725, 740, 1077
PspPPI RGGWCCY 1 cut(s) 1077
PsuI RGATCY 1 cut(s) 439
PsyI GACNNNGTC 1 cut(s) 541
RsaI GTAC 5 cut(s) 188, 574, 661, 981, 1031
RsaNI GTAC 5 cut(s) 187, 573, 660, 980, 1030
RseI CAYNNNNRTG 1 cut(s) 638
SatI GCNGC 7 cut(s) 306, 309, 336, 363, 421, 424, 510
Sau3AI GATC 7 cut(s) 77, 400, 439, 808, 1129, 1135, 1350
Sau96I GGNCC 7 cut(s) 265, 266, 394, 483, 725, 740, 1077
ScaI AGTACT 1 cut(s) 574
SchI GAGTC 3 cut(s) 66, 383, 1315
ScrFI CCNGG 3 cut(s) 529, 729, 925
SduI GDGCHC 7 cut(s) 115, 146, 269, 506, 604, 893, 1233
SfaNI GCATC 1 cut(s) 656
SinI GGWCC 3 cut(s) 394, 740, 1077
SmiMI CAYNNNNRTG 1 cut(s) 638
SmlI CTYRAG 1 cut(s) 612
SmoI CTYRAG 1 cut(s) 612
SnaBI TACGTA 1 cut(s) 186
Sse9I AATT 9 cut(s) 103, 116, 129, 206, 210, 230, 768, 993, 1388
SspMI CTAG 1 cut(s) 1122
StyD4I CCNGG 3 cut(s) 527, 727, 923
StyI CCWWGG 2 cut(s) 249, 1107
TaaI ACNGT 4 cut(s) 274, 358, 375, 1235
TaiI ACGT 4 cut(s) 188, 911, 933, 1419
TaqI TCGA 2 cut(s) 1210, 1305
TasI AATT 9 cut(s) 103, 116, 129, 206, 210, 230, 768, 993, 1388
TatI WGTACW 3 cut(s) 572, 979, 1029
TauI GCSGC 6 cut(s) 308, 311, 338, 423, 426, 512
TfiI GAWTC 1 cut(s) 341
TscAI CASTG 2 cut(s) 1145, 1238
TseFI GTSAC 5 cut(s) 274, 352, 631, 640, 1318
TseI GCWGC 1 cut(s) 362
Tsp45I GTSAC 5 cut(s) 274, 352, 631, 640, 1318
TspDTI ATGAA 7 cut(s) 283, 578, 918, 1359, 1382, 1440, 1441
TspGWI ACGGA 1 cut(s) 224
TspRI CASTG 2 cut(s) 1145, 1238
Tth111I GACNNNGTC 1 cut(s) 541
VneI GTGCAC 1 cut(s) 502
VpaK11BI GGWCC 3 cut(s) 394, 740, 1077
VspI ATTAAT 1 cut(s) 209
XagI CCTNNNNNAGG 2 cut(s) 947, 1085
XapI RAATTY 3 cut(s) 103, 230, 1388
XceI RCATGY 1 cut(s) 1517
XmnI GAANNNNTTC 1 cut(s) 1448
XspI CTAG 1 cut(s) 1122
ZrmI AGTACT 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.