Plant transposase (Ptta/En/Spm family)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 77 |
| AccIII | TCCGGA | 1 cut(s) | 331 |
| AciI | CCGC | 2 cut(s) | 77, 148 |
| AclWI | GGATC | 2 cut(s) | 410, 423 |
| AcsI | RAATTY | 3 cut(s) | 133, 168, 422 |
| AdeI | CACNNNGTG | 1 cut(s) | 524 |
| AfaI | GTAC | 1 cut(s) | 24 |
| AfeI | AGCGCT | 1 cut(s) | 298 |
| AgsI | TTSAA | 3 cut(s) | 7, 221, 430 |
| AjiI | CACGTC | 1 cut(s) | 524 |
| AjnI | CCWGG | 1 cut(s) | 189 |
| AluBI | AGCT | 4 cut(s) | 13, 19, 319, 455 |
| AluI | AGCT | 4 cut(s) | 13, 19, 319, 455 |
| Alw26I | GTCTC | 3 cut(s) | 173, 192, 257 |
| AlwI | GGATC | 2 cut(s) | 410, 423 |
| Ama87I | CYCGRG | 3 cut(s) | 173, 362, 456 |
| Aor13HI | TCCGGA | 1 cut(s) | 331 |
| Aor51HI | AGCGCT | 1 cut(s) | 298 |
| ApeKI | GCWGC | 2 cut(s) | 91, 440 |
| ApoI | RAATTY | 3 cut(s) | 133, 168, 422 |
| AspLEI | GCGC | 1 cut(s) | 299 |
| AspS9I | GGNCC | 1 cut(s) | 186 |
| AsuII | TTCGAA | 1 cut(s) | 491 |
| AvaI | CYCGRG | 3 cut(s) | 173, 362, 456 |
| AvaII | GGWCC | 1 cut(s) | 186 |
| AxyI | CCTNAGG | 1 cut(s) | 240 |
| BamHI | GGATCC | 1 cut(s) | 415 |
| BbvI | GCAGC | 2 cut(s) | 103, 427 |
| BceAI | ACGGC | 1 cut(s) | 392 |
| BciT130I | CCWGG | 1 cut(s) | 191 |
| BclI | TGATCA | 1 cut(s) | 42 |
| BcoDI | GTCTC | 3 cut(s) | 173, 192, 257 |
| BfmI | CTRYAG | 2 cut(s) | 14, 347 |
| BfoI | RGCGCY | 1 cut(s) | 300 |
| BisI | GCNGC | 2 cut(s) | 92, 441 |
| BlsI | GCNGC | 2 cut(s) | 93, 442 |
| Bme1390I | CCNGG | 1 cut(s) | 191 |
| Bme18I | GGWCC | 1 cut(s) | 186 |
| BmeT110I | CYCGRG | 3 cut(s) | 173, 362, 456 |
| BmgBI | CACGTC | 1 cut(s) | 524 |
| BmgT120I | GGNCC | 1 cut(s) | 186 |
| BmiI | GGNNCC | 2 cut(s) | 188, 417 |
| BmrFI | CCNGG | 1 cut(s) | 191 |
| Bpu14I | TTCGAA | 1 cut(s) | 491 |
| BsaAI | YACGTR | 1 cut(s) | 26 |
| BsaJI | CCNNGG | 2 cut(s) | 174, 189 |
| BsaWI | WCCGGW | 2 cut(s) | 331, 485 |
| Bse118I | RCCGGY | 1 cut(s) | 276 |
| Bse21I | CCTNAGG | 1 cut(s) | 240 |
| BseAI | TCCGGA | 1 cut(s) | 331 |
| BseBI | CCWGG | 1 cut(s) | 191 |
| BseDI | CCNNGG | 2 cut(s) | 174, 189 |
| BseMII | CTCAG | 1 cut(s) | 231 |
| BseRI | GAGGAG | 2 cut(s) | 191, 275 |
| BseXI | GCAGC | 2 cut(s) | 103, 427 |
| BsgI | GTGCAG | 1 cut(s) | 385 |
| BsiHKCI | CYCGRG | 3 cut(s) | 173, 362, 456 |
| BsiSI | CCGG | 3 cut(s) | 277, 332, 486 |
| BslFI | GGGAC | 1 cut(s) | 473 |
| BsmAI | GTCTC | 3 cut(s) | 173, 192, 257 |
| BsmFI | GGGAC | 1 cut(s) | 473 |
| BsoBI | CYCGRG | 3 cut(s) | 173, 362, 456 |
| Bsp119I | TTCGAA | 1 cut(s) | 491 |
| Bsp13I | TCCGGA | 1 cut(s) | 331 |
| Bsp143I | GATC | 2 cut(s) | 42, 415 |
| BspACI | CCGC | 2 cut(s) | 77, 148 |
| BspCNI | CTCAG | 1 cut(s) | 232 |
| BspEI | TCCGGA | 1 cut(s) | 331 |
| BspLI | GGNNCC | 2 cut(s) | 188, 417 |
| BspPI | GGATC | 2 cut(s) | 410, 423 |
| BspQI | GCTCTTC | 2 cut(s) | 287, 306 |
| BspT104I | TTCGAA | 1 cut(s) | 491 |
| BsrBI | CCGCTC | 1 cut(s) | 77 |
| BsrFI | RCCGGY | 1 cut(s) | 276 |
| BssAI | RCCGGY | 1 cut(s) | 276 |
| BssECI | CCNNGG | 2 cut(s) | 174, 189 |
| BssMI | GATC | 2 cut(s) | 42, 415 |
| Bst2UI | CCWGG | 1 cut(s) | 191 |
| Bst6I | CTCTTC | 2 cut(s) | 287, 306 |
| BstBAI | YACGTR | 1 cut(s) | 26 |
| BstBI | TTCGAA | 1 cut(s) | 491 |
| BstC8I | GCNNGC | 3 cut(s) | 274, 343, 445 |
| BstDEI | CTNAG | 2 cut(s) | 240, 256 |
| BstEII | GGTNACC | 1 cut(s) | 79 |
| BstH2I | RGCGCY | 1 cut(s) | 300 |
| BstHHI | GCGC | 1 cut(s) | 299 |
| BstKTI | GATC | 2 cut(s) | 45, 418 |
| BstMAI | GTCTC | 3 cut(s) | 173, 192, 257 |
| BstMBI | GATC | 2 cut(s) | 42, 415 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 316 |
| BstNI | CCWGG | 1 cut(s) | 191 |
| BstPI | GGTNACC | 1 cut(s) | 79 |
| BstSCI | CCNGG | 1 cut(s) | 189 |
| BstSFI | CTRYAG | 2 cut(s) | 14, 347 |
| BstSNI | TACGTA | 1 cut(s) | 26 |
| BstV1I | GCAGC | 2 cut(s) | 103, 427 |
| BstX2I | RGATCY | 1 cut(s) | 415 |
| BstYI | RGATCY | 1 cut(s) | 415 |
| Bsu36I | CCTNAGG | 1 cut(s) | 240 |
| BtrI | CACGTC | 1 cut(s) | 524 |
| BtsIMutI | CAGTG | 1 cut(s) | 253 |
| Cac8I | GCNNGC | 3 cut(s) | 274, 343, 445 |
| CfoI | GCGC | 1 cut(s) | 299 |
| Cfr10I | RCCGGY | 1 cut(s) | 276 |
| Cfr13I | GGNCC | 1 cut(s) | 186 |
| CseI | GACGC | 1 cut(s) | 91 |
| Csp6I | GTAC | 1 cut(s) | 23 |
| CviJI | RGCY | 7 cut(s) | 13, 19, 276, 319, 397, 455, 496 |
| CviKI_1 | RGCY | 7 cut(s) | 13, 19, 276, 319, 397, 455, 496 |
| CviQI | GTAC | 1 cut(s) | 23 |
| DdeI | CTNAG | 2 cut(s) | 240, 256 |
| DpnI | GATC | 2 cut(s) | 44, 417 |
| DpnII | GATC | 2 cut(s) | 42, 415 |
| DraIII | CACNNNGTG | 1 cut(s) | 524 |
| Eam1104I | CTCTTC | 2 cut(s) | 287, 306 |
| EarI | CTCTTC | 2 cut(s) | 287, 306 |
| Eco105I | TACGTA | 1 cut(s) | 26 |
| Eco47I | GGWCC | 1 cut(s) | 186 |
| Eco47III | AGCGCT | 1 cut(s) | 298 |
| Eco81I | CCTNAGG | 1 cut(s) | 240 |
| Eco88I | CYCGRG | 3 cut(s) | 173, 362, 456 |
| Eco91I | GGTNACC | 1 cut(s) | 79 |
| EcoO109I | RGGNCCY | 1 cut(s) | 186 |
| EcoO65I | GGTNACC | 1 cut(s) | 79 |
| EcoRII | CCWGG | 1 cut(s) | 189 |
| FaqI | GGGAC | 1 cut(s) | 473 |
| FbaI | TGATCA | 1 cut(s) | 42 |
| Fnu4HI | GCNGC | 2 cut(s) | 92, 441 |
| Fsp4HI | GCNGC | 2 cut(s) | 92, 441 |
| GlaI | GCGC | 1 cut(s) | 298 |
| GluI | GCNGC | 2 cut(s) | 92, 441 |
| HaeII | RGCGCY | 1 cut(s) | 300 |
| HapII | CCGG | 3 cut(s) | 277, 332, 486 |
| HgaI | GACGC | 1 cut(s) | 91 |
| HhaI | GCGC | 1 cut(s) | 299 |
| Hin6I | GCGC | 1 cut(s) | 297 |
| HinP1I | GCGC | 1 cut(s) | 297 |
| HinfI | GANTC | 2 cut(s) | 326, 461 |
| HpaII | CCGG | 3 cut(s) | 277, 332, 486 |
| Hpy188III | TCNNGA | 4 cut(s) | 46, 173, 332, 458 |
| Hpy99I | CGWCG | 2 cut(s) | 107, 525 |
| HpyCH4IV | ACGT | 2 cut(s) | 25, 523 |
| HpyCH4V | TGCA | 1 cut(s) | 402 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 316 |
| HpyF3I | CTNAG | 2 cut(s) | 240, 256 |
| HpySE526I | ACGT | 2 cut(s) | 25, 523 |
| HspAI | GCGC | 1 cut(s) | 297 |
| Kpn2I | TCCGGA | 1 cut(s) | 331 |
| Ksp22I | TGATCA | 1 cut(s) | 42 |
| Kzo9I | GATC | 2 cut(s) | 42, 415 |
| LguI | GCTCTTC | 2 cut(s) | 287, 306 |
| LmnI | GCTCC | 2 cut(s) | 10, 74 |
| LpnPI | CCDG | 8 cut(s) | 176, 203, 252, 290, 335, 345, 429, 499 |
| Lsp1109I | GCAGC | 2 cut(s) | 103, 427 |
| MaeII | ACGT | 2 cut(s) | 25, 523 |
| MaeIII | GTNAC | 1 cut(s) | 79 |
| MalI | GATC | 2 cut(s) | 44, 417 |
| MbiI | CCGCTC | 1 cut(s) | 77 |
| MboI | GATC | 2 cut(s) | 42, 415 |
| MboII | GAAGA | 3 cut(s) | 293, 304, 442 |
| MflI | RGATCY | 1 cut(s) | 415 |
| MluCI | AATT | 5 cut(s) | 109, 133, 168, 202, 422 |
| MlyI | GAGTC | 1 cut(s) | 455 |
| MroI | TCCGGA | 1 cut(s) | 331 |
| MseI | TTAA | 2 cut(s) | 108, 541 |
| MspI | CCGG | 3 cut(s) | 277, 332, 486 |
| MspR9I | CCNGG | 1 cut(s) | 191 |
| MvaI | CCWGG | 1 cut(s) | 191 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 316 |
| NdeII | GATC | 2 cut(s) | 42, 415 |
| NlaIV | GGNNCC | 2 cut(s) | 188, 417 |
| NspV | TTCGAA | 1 cut(s) | 491 |
| PaeR7I | CTCGAG | 1 cut(s) | 362 |
| PciSI | GCTCTTC | 2 cut(s) | 287, 306 |
| PfeI | GAWTC | 1 cut(s) | 326 |
| PkrI | GCNGC | 2 cut(s) | 93, 442 |
| PleI | GAGTC | 1 cut(s) | 455 |
| PpsI | GAGTC | 1 cut(s) | 455 |
| Ppu21I | YACGTR | 1 cut(s) | 26 |
| PpuMI | RGGWCCY | 1 cut(s) | 186 |
| Psp5II | RGGWCCY | 1 cut(s) | 186 |
| Psp6I | CCWGG | 1 cut(s) | 189 |
| PspEI | GGTNACC | 1 cut(s) | 79 |
| PspGI | CCWGG | 1 cut(s) | 189 |
| PspN4I | GGNNCC | 2 cut(s) | 188, 417 |
| PspPI | GGNCC | 1 cut(s) | 186 |
| PspPPI | RGGWCCY | 1 cut(s) | 186 |
| PspXI | VCTCGAGB | 1 cut(s) | 362 |
| PsuI | RGATCY | 1 cut(s) | 415 |
| RsaI | GTAC | 1 cut(s) | 24 |
| RsaNI | GTAC | 1 cut(s) | 23 |
| SapI | GCTCTTC | 2 cut(s) | 287, 306 |
| SaqAI | TTAA | 2 cut(s) | 108, 541 |
| SatI | GCNGC | 2 cut(s) | 92, 441 |
| Sau3AI | GATC | 2 cut(s) | 42, 415 |
| Sau96I | GGNCC | 1 cut(s) | 186 |
| SchI | GAGTC | 1 cut(s) | 455 |
| ScrFI | CCNGG | 1 cut(s) | 191 |
| SfcI | CTRYAG | 2 cut(s) | 14, 347 |
| Sfr274I | CTCGAG | 1 cut(s) | 362 |
| SfuI | TTCGAA | 1 cut(s) | 491 |
| SinI | GGWCC | 1 cut(s) | 186 |
| SlaI | CTCGAG | 1 cut(s) | 362 |
| SmlI | CTYRAG | 1 cut(s) | 362 |
| SmoI | CTYRAG | 1 cut(s) | 362 |
| SnaBI | TACGTA | 1 cut(s) | 26 |
| Sse9I | AATT | 5 cut(s) | 109, 133, 168, 202, 422 |
| SsiI | CCGC | 2 cut(s) | 77, 148 |
| StyD4I | CCNGG | 1 cut(s) | 189 |
| TaiI | ACGT | 2 cut(s) | 28, 526 |
| TaqI | TCGA | 2 cut(s) | 363, 491 |
| TasI | AATT | 5 cut(s) | 109, 133, 168, 202, 422 |
| TfiI | GAWTC | 1 cut(s) | 326 |
| Tru1I | TTAA | 2 cut(s) | 108, 541 |
| Tru9I | TTAA | 2 cut(s) | 108, 541 |
| TscAI | CASTG | 1 cut(s) | 253 |
| TseI | GCWGC | 2 cut(s) | 91, 440 |
| TspGWI | ACGGA | 1 cut(s) | 428 |
| TspRI | CASTG | 1 cut(s) | 253 |
| VpaK11BI | GGWCC | 1 cut(s) | 186 |
| XapI | RAATTY | 3 cut(s) | 133, 168, 422 |
| XhoI | CTCGAG | 1 cut(s) | 362 |