C1 domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1239 |
| Acc36I | ACCTGC | 1 cut(s) | 769 |
| AccB1I | GGYRCC | 2 cut(s) | 81, 519 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 98 |
| AccBSI | CCGCTC | 4 cut(s) | 181, 499, 1687, 1729 |
| AccII | CGCG | 3 cut(s) | 67, 234, 1571 |
| AclWI | GGATC | 4 cut(s) | 12, 83, 1412, 1701 |
| AcoI | YGGCCR | 1 cut(s) | 412 |
| AcsI | RAATTY | 4 cut(s) | 12, 392, 1243, 1746 |
| AcuI | CTGAAG | 2 cut(s) | 775, 1237 |
| AfaI | GTAC | 4 cut(s) | 563, 1231, 1325, 1388 |
| AfeI | AGCGCT | 1 cut(s) | 1595 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 98, 275, 378, 832, 894, 987, 1030 |
| AflII | CTTAAG | 1 cut(s) | 676 |
| AflIII | ACRYGT | 2 cut(s) | 120, 1065 |
| AgsI | TTSAA | 5 cut(s) | 37, 337, 392, 434, 644 |
| AhlI | ACTAGT | 1 cut(s) | 1232 |
| AjnI | CCWGG | 1 cut(s) | 1122 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 975, 1007 |
| AluBI | AGCT | 5 cut(s) | 287, 466, 538, 1015, 1207 |
| AluI | AGCT | 5 cut(s) | 287, 466, 538, 1015, 1207 |
| Alw21I | GWGCWC | 6 cut(s) | 363, 789, 1017, 1215, 1533, 1683 |
| Alw26I | GTCTC | 1 cut(s) | 743 |
| Alw44I | GTGCAC | 1 cut(s) | 1211 |
| AlwI | GGATC | 4 cut(s) | 12, 83, 1412, 1701 |
| Aor51HI | AGCGCT | 1 cut(s) | 1595 |
| AoxI | GGCC | 5 cut(s) | 101, 221, 383, 412, 823 |
| ApaLI | GTGCAC | 1 cut(s) | 1211 |
| ApeKI | GCWGC | 2 cut(s) | 659, 1218 |
| ApoI | RAATTY | 4 cut(s) | 12, 392, 1243, 1746 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 337, 369 |
| AseI | ATTAAT | 1 cut(s) | 1665 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 279 |
| AspLEI | GCGC | 2 cut(s) | 1571, 1596 |
| AspS9I | GGNCC | 5 cut(s) | 59, 101, 221, 384, 1126 |
| AsuC2I | CCSGG | 2 cut(s) | 1445, 1676 |
| AsuHPI | GGTGA | 2 cut(s) | 361, 911 |
| AvaII | GGWCC | 2 cut(s) | 59, 1126 |
| BaeGI | GKGCMC | 1 cut(s) | 1215 |
| BalI | TGGCCA | 1 cut(s) | 414 |
| BamHI | GGATCC | 1 cut(s) | 4 |
| BanI | GGYRCC | 2 cut(s) | 81, 519 |
| BanII | GRGCYC | 2 cut(s) | 1017, 1514 |
| BauI | CACGAG | 3 cut(s) | 1010, 1208, 1682 |
| Bbv12I | GWGCWC | 6 cut(s) | 363, 789, 1017, 1215, 1533, 1683 |
| BbvI | GCAGC | 2 cut(s) | 671, 1230 |
| BccI | CCATC | 5 cut(s) | 50, 483, 727, 815, 896 |
| BceAI | ACGGC | 1 cut(s) | 533 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1189, 1223 |
| BciT130I | CCWGG | 1 cut(s) | 1124 |
| BciVI | GTATCC | 1 cut(s) | 1500 |
| BclI | TGATCA | 1 cut(s) | 763 |
| BcnI | CCSGG | 2 cut(s) | 1445, 1676 |
| BcoDI | GTCTC | 1 cut(s) | 743 |
| BcuI | ACTAGT | 1 cut(s) | 1232 |
| BfaI | CTAG | 6 cut(s) | 86, 215, 284, 827, 1233, 1362 |
| BfmI | CTRYAG | 2 cut(s) | 776, 1216 |
| BfoI | RGCGCY | 1 cut(s) | 1597 |
| BfrI | CTTAAG | 1 cut(s) | 676 |
| BfuAI | ACCTGC | 1 cut(s) | 769 |
| BfuI | GTATCC | 1 cut(s) | 1500 |
| BglII | AGATCT | 1 cut(s) | 168 |
| BisI | GCNGC | 3 cut(s) | 235, 660, 1219 |
| BlsI | GCNGC | 3 cut(s) | 236, 661, 1220 |
| BmcAI | AGTACT | 1 cut(s) | 1388 |
| Bme1390I | CCNGG | 3 cut(s) | 1124, 1445, 1676 |
| Bme18I | GGWCC | 2 cut(s) | 59, 1126 |
| BmgT120I | GGNCC | 5 cut(s) | 59, 101, 221, 384, 1126 |
| BmiI | GGNNCC | 5 cut(s) | 6, 83, 238, 521, 551 |
| BmrFI | CCNGG | 3 cut(s) | 1124, 1445, 1676 |
| BmsI | GCATC | 5 cut(s) | 31, 293, 456, 1043, 1162 |
| BpmI | CTGGAG | 1 cut(s) | 1175 |
| BpuEI | CTTGAG | 2 cut(s) | 518, 1099 |
| BpuMI | CCSGG | 2 cut(s) | 1445, 1676 |
| BsaAI | YACGTR | 1 cut(s) | 543 |
| BsaBI | GATNNNNATC | 3 cut(s) | 320, 426, 1464 |
| BsaI | GGTCTC | 1 cut(s) | 743 |
| BsaJI | CCNNGG | 3 cut(s) | 65, 232, 1084 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 221, 251 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 98, 275, 378, 832, 894, 987, 1030 |
| Bse1I | ACTGG | 3 cut(s) | 92, 259, 1192 |
| Bse3DI | GCAATG | 1 cut(s) | 1471 |
| Bse8I | GATNNNNATC | 3 cut(s) | 320, 426, 1464 |
| BseBI | CCWGG | 1 cut(s) | 1124 |
| BseDI | CCNNGG | 3 cut(s) | 65, 232, 1084 |
| BseGI | GGATG | 8 cut(s) | 22, 427, 702, 719, 725, 1339, 1465, 1741 |
| BseJI | GATNNNNATC | 3 cut(s) | 320, 426, 1464 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 98, 275, 378, 832, 894, 987, 1030 |
| BseMI | GCAATG | 1 cut(s) | 1471 |
| BseMII | CTCAG | 1 cut(s) | 220 |
| BseNI | ACTGG | 3 cut(s) | 92, 259, 1192 |
| BseRI | GAGGAG | 2 cut(s) | 59, 62 |
| BseSI | GKGCMC | 1 cut(s) | 1215 |
| BseXI | GCAGC | 2 cut(s) | 671, 1230 |
| BseYI | CCCAGC | 1 cut(s) | 739 |
| Bsh1236I | CGCG | 3 cut(s) | 67, 234, 1571 |
| BshFI | GGCC | 5 cut(s) | 103, 223, 385, 414, 825 |
| BshNI | GGYRCC | 2 cut(s) | 81, 519 |
| BsiHKAI | GWGCWC | 6 cut(s) | 363, 789, 1017, 1215, 1533, 1683 |
| BsiSI | CCGG | 2 cut(s) | 1445, 1675 |
| BslFI | GGGAC | 1 cut(s) | 720 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 98, 275, 378, 832, 894, 987, 1030 |
| BsmAI | GTCTC | 1 cut(s) | 743 |
| BsmFI | GGGAC | 1 cut(s) | 720 |
| BsmI | GAATGC | 1 cut(s) | 1476 |
| BsnI | GGCC | 5 cut(s) | 103, 223, 385, 414, 825 |
| Bso31I | GGTCTC | 1 cut(s) | 743 |
| Bsp1286I | GDGCHC | 7 cut(s) | 363, 789, 1017, 1215, 1514, 1533, 1683 |
| Bsp143I | GATC | 9 cut(s) | 4, 88, 168, 637, 763, 1282, 1336, 1417, 1693 |
| BspANI | GGCC | 5 cut(s) | 103, 223, 385, 414, 825 |
| BspCNI | CTCAG | 1 cut(s) | 219 |
| BspFNI | CGCG | 3 cut(s) | 67, 234, 1571 |
| BspHI | TCATGA | 5 cut(s) | 355, 634, 696, 766, 891 |
| BspLI | GGNNCC | 5 cut(s) | 6, 83, 238, 521, 551 |
| BspMAI | CTGCAG | 2 cut(s) | 780, 1220 |
| BspMI | ACCTGC | 1 cut(s) | 769 |
| BspPI | GGATC | 4 cut(s) | 12, 83, 1412, 1701 |
| BspT107I | GGYRCC | 2 cut(s) | 81, 519 |
| BspTI | CTTAAG | 1 cut(s) | 676 |
| BspTNI | GGTCTC | 1 cut(s) | 743 |
| BsrBI | CCGCTC | 4 cut(s) | 181, 499, 1687, 1729 |
| BsrDI | GCAATG | 1 cut(s) | 1471 |
| BsrI | ACTGG | 3 cut(s) | 92, 259, 1192 |
| BssECI | CCNNGG | 3 cut(s) | 65, 232, 1084 |
| BssMI | GATC | 9 cut(s) | 4, 88, 168, 637, 763, 1282, 1336, 1417, 1693 |
| BssSI | CACGAG | 3 cut(s) | 1010, 1208, 1682 |
| BssT1I | CCWWGG | 1 cut(s) | 1084 |
| Bst2BI | CACGAG | 3 cut(s) | 1010, 1208, 1682 |
| Bst2UI | CCWGG | 1 cut(s) | 1124 |
| Bst4CI | ACNGT | 6 cut(s) | 353, 617, 668, 875, 1100, 1318 |
| Bst6I | CTCTTC | 1 cut(s) | 36 |
| BstAFI | CTTAAG | 1 cut(s) | 676 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 876 |
| BstBAI | YACGTR | 1 cut(s) | 543 |
| BstC8I | GCNNGC | 2 cut(s) | 464, 881 |
| BstDEI | CTNAG | 2 cut(s) | 206, 1620 |
| BstDSI | CCRYGG | 2 cut(s) | 65, 232 |
| BstEII | GGTNACC | 1 cut(s) | 492 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1028 |
| BstF5I | GGATG | 8 cut(s) | 22, 427, 702, 719, 725, 1339, 1465, 1741 |
| BstFNI | CGCG | 3 cut(s) | 67, 234, 1571 |
| BstH2I | RGCGCY | 1 cut(s) | 1597 |
| BstHHI | GCGC | 2 cut(s) | 1571, 1596 |
| BstKTI | GATC | 9 cut(s) | 7, 91, 171, 640, 766, 1285, 1339, 1420, 1696 |
| BstMAI | GTCTC | 1 cut(s) | 743 |
| BstMBI | GATC | 9 cut(s) | 4, 88, 168, 637, 763, 1282, 1336, 1417, 1693 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 784, 876, 1528 |
| BstNI | CCWGG | 1 cut(s) | 1124 |
| BstNSI | RCATGY | 4 cut(s) | 124, 486, 1069, 1769 |
| BstPI | GGTNACC | 1 cut(s) | 492 |
| BstSCI | CCNGG | 3 cut(s) | 1122, 1443, 1674 |
| BstSFI | CTRYAG | 2 cut(s) | 776, 1216 |
| BstSLI | GKGCMC | 1 cut(s) | 1215 |
| BstUI | CGCG | 3 cut(s) | 67, 234, 1571 |
| BstV1I | GCAGC | 2 cut(s) | 671, 1230 |
| BstX2I | RGATCY | 4 cut(s) | 4, 88, 168, 1417 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1428 |
| BstYI | RGATCY | 4 cut(s) | 4, 88, 168, 1417 |
| BsuI | GTATCC | 1 cut(s) | 1500 |
| BsuRI | GGCC | 5 cut(s) | 103, 223, 385, 414, 825 |
| BtgI | CCRYGG | 2 cut(s) | 65, 232 |
| BtgZI | GCGATG | 2 cut(s) | 417, 461 |
| BtsCI | GGATG | 8 cut(s) | 22, 427, 702, 719, 725, 1339, 1465, 1741 |
| BtsI | GCAGTG | 1 cut(s) | 1213 |
| BtsIMutI | CAGTG | 3 cut(s) | 99, 664, 1213 |
| BveI | ACCTGC | 1 cut(s) | 769 |
| Cac8I | GCNNGC | 2 cut(s) | 464, 881 |
| CciI | TCATGA | 5 cut(s) | 355, 634, 696, 766, 891 |
| CfoI | GCGC | 2 cut(s) | 1571, 1596 |
| Cfr13I | GGNCC | 5 cut(s) | 59, 101, 221, 384, 1126 |
| Cfr42I | CCGCGG | 2 cut(s) | 68, 235 |
| CseI | GACGC | 1 cut(s) | 1168 |
| Csp6I | GTAC | 4 cut(s) | 562, 1230, 1324, 1387 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 947, 982 |
| CviQI | GTAC | 4 cut(s) | 562, 1230, 1324, 1387 |
| DdeI | CTNAG | 2 cut(s) | 206, 1620 |
| DpnI | GATC | 9 cut(s) | 6, 90, 170, 639, 765, 1284, 1338, 1419, 1695 |
| DpnII | GATC | 9 cut(s) | 4, 88, 168, 637, 763, 1282, 1336, 1417, 1693 |
| EaeI | YGGCCR | 1 cut(s) | 412 |
| Eam1104I | CTCTTC | 1 cut(s) | 36 |
| EarI | CTCTTC | 1 cut(s) | 36 |
| Ecl136II | GAGCTC | 1 cut(s) | 1015 |
| Eco130I | CCWWGG | 1 cut(s) | 1084 |
| Eco24I | GRGCYC | 2 cut(s) | 1017, 1514 |
| Eco31I | GGTCTC | 1 cut(s) | 743 |
| Eco47I | GGWCC | 2 cut(s) | 59, 1126 |
| Eco47III | AGCGCT | 1 cut(s) | 1595 |
| Eco53kI | GAGCTC | 1 cut(s) | 1015 |
| Eco57I | CTGAAG | 2 cut(s) | 775, 1237 |
| Eco91I | GGTNACC | 1 cut(s) | 492 |
| EcoICRI | GAGCTC | 1 cut(s) | 1015 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1028 |
| EcoO65I | GGTNACC | 1 cut(s) | 492 |
| EcoRI | GAATTC | 3 cut(s) | 12, 392, 1746 |
| EcoRII | CCWGG | 1 cut(s) | 1122 |
| EcoT14I | CCWWGG | 1 cut(s) | 1084 |
| EcoT38I | GRGCYC | 2 cut(s) | 1017, 1514 |
| ErhI | CCWWGG | 1 cut(s) | 1084 |
| FaqI | GGGAC | 1 cut(s) | 720 |
| FauI | CCCGC | 3 cut(s) | 186, 1680, 1722 |
| FauNDI | CATATG | 1 cut(s) | 1400 |
| FbaI | TGATCA | 1 cut(s) | 763 |
| Fnu4HI | GCNGC | 3 cut(s) | 235, 660, 1219 |
| FokI | GGATG | 8 cut(s) | 9, 434, 689, 706, 712, 1326, 1452, 1748 |
| FriOI | GRGCYC | 2 cut(s) | 1017, 1514 |
| Fsp4HI | GCNGC | 3 cut(s) | 235, 660, 1219 |
| FspBI | CTAG | 6 cut(s) | 86, 215, 284, 827, 1233, 1362 |
| GlaI | GCGC | 2 cut(s) | 1570, 1595 |
| GluI | GCNGC | 3 cut(s) | 235, 660, 1219 |
| GsaI | CCCAGC | 1 cut(s) | 743 |
| GsuI | CTGGAG | 1 cut(s) | 1175 |
| HaeII | RGCGCY | 1 cut(s) | 1597 |
| HaeIII | GGCC | 5 cut(s) | 103, 223, 385, 414, 825 |
| HapII | CCGG | 2 cut(s) | 1445, 1675 |
| HgaI | GACGC | 1 cut(s) | 1168 |
| HhaI | GCGC | 2 cut(s) | 1571, 1596 |
| Hin6I | GCGC | 2 cut(s) | 1569, 1594 |
| HinP1I | GCGC | 2 cut(s) | 1569, 1594 |
| HincII | GTYRAC | 1 cut(s) | 810 |
| HindII | GTYRAC | 1 cut(s) | 810 |
| HindIII | AAGCTT | 1 cut(s) | 536 |
| HinfI | GANTC | 3 cut(s) | 902, 1073, 1573 |
| HpaI | GTTAAC | 1 cut(s) | 810 |
| HpaII | CCGG | 2 cut(s) | 1445, 1675 |
| HphI | GGTGA | 2 cut(s) | 361, 911 |
| Hpy166II | GTNNAC | 4 cut(s) | 62, 810, 1183, 1213 |
| Hpy188I | TCNGA | 4 cut(s) | 209, 302, 1256, 1723 |
| Hpy8I | GTNNAC | 4 cut(s) | 62, 810, 1183, 1213 |
| Hpy99I | CGWCG | 1 cut(s) | 353 |
| HpyAV | CCTTC | 5 cut(s) | 269, 851, 1034, 1400, 1625 |
| HpyCH4III | ACNGT | 6 cut(s) | 353, 617, 668, 875, 1100, 1318 |
| HpyCH4IV | ACGT | 2 cut(s) | 542, 1740 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 784, 876, 1528 |
| HpyF3I | CTNAG | 2 cut(s) | 206, 1620 |
| HpySE526I | ACGT | 2 cut(s) | 542, 1740 |
| HspAI | GCGC | 2 cut(s) | 1569, 1594 |
| Ksp22I | TGATCA | 1 cut(s) | 763 |
| KspAI | GTTAAC | 1 cut(s) | 810 |
| KspI | CCGCGG | 2 cut(s) | 68, 235 |
| Kzo9I | GATC | 9 cut(s) | 4, 88, 168, 637, 763, 1282, 1336, 1417, 1693 |
| LmnI | GCTCC | 5 cut(s) | 186, 242, 504, 1167, 1678 |
| Lsp1109I | GCAGC | 2 cut(s) | 671, 1230 |
| LweI | GCATC | 5 cut(s) | 31, 293, 456, 1043, 1162 |
| MaeI | CTAG | 6 cut(s) | 86, 215, 284, 827, 1233, 1362 |
| MaeII | ACGT | 2 cut(s) | 542, 1740 |
| MaeIII | GTNAC | 6 cut(s) | 156, 492, 584, 1061, 1394, 1451 |
| MalI | GATC | 9 cut(s) | 6, 90, 170, 639, 765, 1284, 1338, 1419, 1695 |
| MbiI | CCGCTC | 4 cut(s) | 181, 499, 1687, 1729 |
| MboI | GATC | 9 cut(s) | 4, 88, 168, 637, 763, 1282, 1336, 1417, 1693 |
| MboII | GAAGA | 2 cut(s) | 53, 422 |
| MflI | RGATCY | 4 cut(s) | 4, 88, 168, 1417 |
| MhlI | GDGCHC | 7 cut(s) | 363, 789, 1017, 1215, 1514, 1533, 1683 |
| MlsI | TGGCCA | 1 cut(s) | 414 |
| MluNI | TGGCCA | 1 cut(s) | 414 |
| MlyI | GAGTC | 1 cut(s) | 911 |
| Mox20I | TGGCCA | 1 cut(s) | 414 |
| MroXI | GAANNNNTTC | 1 cut(s) | 279 |
| MscI | TGGCCA | 1 cut(s) | 414 |
| MseI | TTAA | 9 cut(s) | 677, 809, 1056, 1302, 1368, 1598, 1665, 1712, 1777 |
| MslI | CAYNNNNRTG | 1 cut(s) | 730 |
| Msp20I | TGGCCA | 1 cut(s) | 414 |
| MspA1I | CMGCKG | 3 cut(s) | 67, 234, 758 |
| MspCI | CTTAAG | 1 cut(s) | 676 |
| MspI | CCGG | 2 cut(s) | 1445, 1675 |
| MspR9I | CCNGG | 3 cut(s) | 1124, 1445, 1676 |
| Mva1269I | GAATGC | 1 cut(s) | 1476 |
| MvaI | CCWGG | 1 cut(s) | 1124 |
| MvnI | CGCG | 3 cut(s) | 67, 234, 1571 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 784, 876, 1528 |
| NciI | CCSGG | 2 cut(s) | 1445, 1676 |
| NdeI | CATATG | 1 cut(s) | 1400 |
| NdeII | GATC | 9 cut(s) | 4, 88, 168, 637, 763, 1282, 1336, 1417, 1693 |
| NlaIV | GGNNCC | 5 cut(s) | 6, 83, 238, 521, 551 |
| NmuCI | GTSAC | 4 cut(s) | 584, 1061, 1394, 1451 |
| NspI | RCATGY | 4 cut(s) | 124, 486, 1069, 1769 |
| PagI | TCATGA | 5 cut(s) | 355, 634, 696, 766, 891 |
| PciI | ACATGT | 2 cut(s) | 120, 1065 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1702 |
| PctI | GAATGC | 1 cut(s) | 1476 |
| PdmI | GAANNNNTTC | 1 cut(s) | 279 |
| PfeI | GAWTC | 2 cut(s) | 1073, 1573 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 98 |
| PkrI | GCNGC | 3 cut(s) | 236, 661, 1220 |
| PleI | GAGTC | 1 cut(s) | 910 |
| PpsI | GAGTC | 1 cut(s) | 910 |
| Ppu21I | YACGTR | 1 cut(s) | 543 |
| PscI | ACATGT | 2 cut(s) | 120, 1065 |
| PshBI | ATTAAT | 1 cut(s) | 1665 |
| PsiI | TTATAA | 1 cut(s) | 1239 |
| Psp124BI | GAGCTC | 1 cut(s) | 1017 |
| Psp6I | CCWGG | 1 cut(s) | 1122 |
| PspEI | GGTNACC | 1 cut(s) | 492 |
| PspFI | CCCAGC | 1 cut(s) | 739 |
| PspGI | CCWGG | 1 cut(s) | 1122 |
| PspN4I | GGNNCC | 5 cut(s) | 6, 83, 238, 521, 551 |
| PspPI | GGNCC | 5 cut(s) | 59, 101, 221, 384, 1126 |
| PstI | CTGCAG | 2 cut(s) | 780, 1220 |
| PsuI | RGATCY | 4 cut(s) | 4, 88, 168, 1417 |
| RsaI | GTAC | 4 cut(s) | 563, 1231, 1325, 1388 |
| RsaNI | GTAC | 4 cut(s) | 562, 1230, 1324, 1387 |
| RseI | CAYNNNNRTG | 1 cut(s) | 730 |
| SacI | GAGCTC | 1 cut(s) | 1017 |
| SacII | CCGCGG | 2 cut(s) | 68, 235 |
| SaqAI | TTAA | 9 cut(s) | 677, 809, 1056, 1302, 1368, 1598, 1665, 1712, 1777 |
| SatI | GCNGC | 3 cut(s) | 235, 660, 1219 |
| Sau3AI | GATC | 9 cut(s) | 4, 88, 168, 637, 763, 1282, 1336, 1417, 1693 |
| Sau96I | GGNCC | 5 cut(s) | 59, 101, 221, 384, 1126 |
| SbfI | CCTGCAGG | 1 cut(s) | 780 |
| ScaI | AGTACT | 1 cut(s) | 1388 |
| SchI | GAGTC | 1 cut(s) | 911 |
| ScrFI | CCNGG | 3 cut(s) | 1124, 1445, 1676 |
| SdaI | CCTGCAGG | 1 cut(s) | 780 |
| SduI | GDGCHC | 7 cut(s) | 363, 789, 1017, 1215, 1514, 1533, 1683 |
| SfaNI | GCATC | 5 cut(s) | 31, 293, 456, 1043, 1162 |
| SfcI | CTRYAG | 2 cut(s) | 776, 1216 |
| Sfr303I | CCGCGG | 2 cut(s) | 68, 235 |
| SgrBI | CCGCGG | 2 cut(s) | 68, 235 |
| SinI | GGWCC | 2 cut(s) | 59, 1126 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 730 |
| SmlI | CTYRAG | 3 cut(s) | 533, 676, 1078 |
| SmoI | CTYRAG | 3 cut(s) | 533, 676, 1078 |
| SpeI | ACTAGT | 1 cut(s) | 1232 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 780 |
| SspMI | CTAG | 6 cut(s) | 86, 215, 284, 827, 1233, 1362 |
| SstI | GAGCTC | 1 cut(s) | 1017 |
| StyD4I | CCNGG | 3 cut(s) | 1122, 1443, 1674 |
| StyI | CCWWGG | 1 cut(s) | 1084 |
| TaaI | ACNGT | 6 cut(s) | 353, 617, 668, 875, 1100, 1318 |
| TaiI | ACGT | 2 cut(s) | 545, 1743 |
| TaqI | TCGA | 5 cut(s) | 711, 783, 1281, 1380, 1480 |
| TatI | WGTACW | 1 cut(s) | 1386 |
| TauI | GCSGC | 1 cut(s) | 237 |
| TfiI | GAWTC | 2 cut(s) | 1073, 1573 |
| Tru1I | TTAA | 9 cut(s) | 677, 809, 1056, 1302, 1368, 1598, 1665, 1712, 1777 |
| Tru9I | TTAA | 9 cut(s) | 677, 809, 1056, 1302, 1368, 1598, 1665, 1712, 1777 |
| TscAI | CASTG | 3 cut(s) | 99, 671, 1220 |
| TseFI | GTSAC | 4 cut(s) | 584, 1061, 1394, 1451 |
| TseI | GCWGC | 2 cut(s) | 659, 1218 |
| Tsp45I | GTSAC | 4 cut(s) | 584, 1061, 1394, 1451 |
| TspDTI | ATGAA | 4 cut(s) | 713, 729, 783, 870 |
| TspGWI | ACGGA | 1 cut(s) | 1593 |
| TspRI | CASTG | 3 cut(s) | 99, 671, 1220 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 98 |
| Vha464I | CTTAAG | 1 cut(s) | 676 |
| VneI | GTGCAC | 1 cut(s) | 1211 |
| VpaK11BI | GGWCC | 2 cut(s) | 59, 1126 |
| VspI | ATTAAT | 1 cut(s) | 1665 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1028 |
| XapI | RAATTY | 4 cut(s) | 12, 392, 1243, 1746 |
| XceI | RCATGY | 4 cut(s) | 124, 486, 1069, 1769 |
| XmnI | GAANNNNTTC | 1 cut(s) | 279 |
| XspI | CTAG | 6 cut(s) | 86, 215, 284, 827, 1233, 1362 |
| ZrmI | AGTACT | 1 cut(s) | 1388 |