C1 domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 642 |
| AccB1I | GGYRCC | 1 cut(s) | 243 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 166 |
| AccII | CGCG | 1 cut(s) | 312 |
| AciI | CCGC | 2 cut(s) | 471, 1108 |
| AcsI | RAATTY | 1 cut(s) | 544 |
| AcuI | CTGAAG | 2 cut(s) | 433, 791 |
| AfaI | GTAC | 3 cut(s) | 377, 998, 1183 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 69, 166, 698, 1304 |
| AflIII | ACRYGT | 1 cut(s) | 144 |
| AgsI | TTSAA | 5 cut(s) | 43, 251, 785, 1092, 1319 |
| AjnI | CCWGG | 2 cut(s) | 380, 548 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 908, 940 |
| AleI | CACNNNNGTG | 1 cut(s) | 98 |
| AluBI | AGCT | 9 cut(s) | 24, 163, 371, 570, 936, 1024, 1051, 1174, 1407 |
| AluI | AGCT | 9 cut(s) | 24, 163, 371, 570, 936, 1024, 1051, 1174, 1407 |
| Alw21I | GWGCWC | 2 cut(s) | 625, 1409 |
| Alw26I | GTCTC | 3 cut(s) | 579, 650, 1398 |
| ApeKI | GCWGC | 2 cut(s) | 570, 1110 |
| ApoI | RAATTY | 1 cut(s) | 544 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1269 |
| AspLEI | GCGC | 3 cut(s) | 62, 185, 314 |
| AsuHPI | GGTGA | 3 cut(s) | 446, 650, 1210 |
| BanI | GGYRCC | 1 cut(s) | 243 |
| BanII | GRGCYC | 2 cut(s) | 691, 1409 |
| BbsI | GAAGAC | 1 cut(s) | 258 |
| Bbv12I | GWGCWC | 2 cut(s) | 625, 1409 |
| BbvI | GCAGC | 2 cut(s) | 557, 1097 |
| BccI | CCATC | 3 cut(s) | 276, 280, 963 |
| BceAI | ACGGC | 1 cut(s) | 919 |
| BciT130I | CCWGG | 2 cut(s) | 382, 550 |
| BclI | TGATCA | 1 cut(s) | 478 |
| BcoDI | GTCTC | 3 cut(s) | 579, 650, 1398 |
| BfaI | CTAG | 4 cut(s) | 776, 1035, 1052, 1175 |
| BisI | GCNGC | 3 cut(s) | 471, 571, 1111 |
| BlsI | GCNGC | 3 cut(s) | 472, 572, 1112 |
| BmcAI | AGTACT | 2 cut(s) | 377, 1183 |
| Bme1390I | CCNGG | 2 cut(s) | 382, 550 |
| BmiI | GGNNCC | 3 cut(s) | 245, 690, 913 |
| BmrFI | CCNGG | 2 cut(s) | 382, 550 |
| BmsI | GCATC | 7 cut(s) | 47, 112, 421, 540, 630, 935, 1186 |
| BpiI | GAAGAC | 1 cut(s) | 258 |
| BpmI | CTGGAG | 1 cut(s) | 142 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1408 |
| BpuEI | CTTGAG | 2 cut(s) | 667, 1004 |
| BsaBI | GATNNNNATC | 1 cut(s) | 954 |
| BsaJI | CCNNGG | 1 cut(s) | 381 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 69, 166, 698, 1304 |
| Bse1I | ACTGG | 3 cut(s) | 125, 474, 667 |
| Bse3DI | GCAATG | 2 cut(s) | 354, 1141 |
| Bse8I | GATNNNNATC | 1 cut(s) | 954 |
| BseBI | CCWGG | 2 cut(s) | 382, 550 |
| BseDI | CCNNGG | 1 cut(s) | 381 |
| BseGI | GGATG | 4 cut(s) | 268, 531, 1012, 1361 |
| BseJI | GATNNNNATC | 1 cut(s) | 954 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 69, 166, 698, 1304 |
| BseMI | GCAATG | 2 cut(s) | 354, 1141 |
| BseMII | CTCAG | 3 cut(s) | 44, 363, 638 |
| BseNI | ACTGG | 3 cut(s) | 125, 474, 667 |
| BseRI | GAGGAG | 1 cut(s) | 857 |
| BseXI | GCAGC | 2 cut(s) | 557, 1097 |
| BsgI | GTGCAG | 2 cut(s) | 556, 832 |
| Bsh1236I | CGCG | 1 cut(s) | 312 |
| BshNI | GGYRCC | 1 cut(s) | 243 |
| BsiHKAI | GWGCWC | 2 cut(s) | 625, 1409 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 69, 166, 698, 1304 |
| BsmAI | GTCTC | 3 cut(s) | 579, 650, 1398 |
| BsmBI | CGTCTC | 2 cut(s) | 579, 650 |
| BsmI | GAATGC | 3 cut(s) | 185, 297, 1146 |
| Bsp1286I | GDGCHC | 3 cut(s) | 625, 691, 1409 |
| Bsp143I | GATC | 4 cut(s) | 438, 478, 802, 1009 |
| BspACI | CCGC | 2 cut(s) | 471, 1108 |
| BspCNI | CTCAG | 3 cut(s) | 45, 364, 637 |
| BspFNI | CGCG | 1 cut(s) | 312 |
| BspLI | GGNNCC | 3 cut(s) | 245, 690, 913 |
| BspQI | GCTCTTC | 1 cut(s) | 1397 |
| BspT107I | GGYRCC | 1 cut(s) | 243 |
| BsrDI | GCAATG | 2 cut(s) | 354, 1141 |
| BsrI | ACTGG | 3 cut(s) | 125, 474, 667 |
| BssECI | CCNNGG | 1 cut(s) | 381 |
| BssMI | GATC | 4 cut(s) | 438, 478, 802, 1009 |
| Bst2UI | CCWGG | 2 cut(s) | 382, 550 |
| Bst4CI | ACNGT | 1 cut(s) | 506 |
| Bst6I | CTCTTC | 2 cut(s) | 1320, 1397 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 285 |
| BstC8I | GCNNGC | 4 cut(s) | 161, 295, 361, 400 |
| BstDEI | CTNAG | 4 cut(s) | 53, 372, 624, 1408 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1302 |
| BstF5I | GGATG | 4 cut(s) | 268, 531, 1012, 1361 |
| BstFNI | CGCG | 1 cut(s) | 312 |
| BstHHI | GCGC | 3 cut(s) | 62, 185, 314 |
| BstKTI | GATC | 4 cut(s) | 441, 481, 805, 1012 |
| BstMAI | GTCTC | 3 cut(s) | 579, 650, 1398 |
| BstMBI | GATC | 4 cut(s) | 438, 478, 802, 1009 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 285, 294, 356, 404, 1171 |
| BstNI | CCWGG | 2 cut(s) | 382, 550 |
| BstNSI | RCATGY | 2 cut(s) | 148, 363 |
| BstSCI | CCNGG | 2 cut(s) | 380, 548 |
| BstUI | CGCG | 1 cut(s) | 312 |
| BstV1I | GCAGC | 2 cut(s) | 557, 1097 |
| BstV2I | GAAGAC | 1 cut(s) | 258 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1289 |
| BtgZI | GCGATG | 3 cut(s) | 209, 342, 845 |
| BtsCI | GGATG | 4 cut(s) | 268, 531, 1012, 1361 |
| BtsIMutI | CAGTG | 3 cut(s) | 481, 719, 815 |
| Cac8I | GCNNGC | 4 cut(s) | 161, 295, 361, 400 |
| CfoI | GCGC | 3 cut(s) | 62, 185, 314 |
| CseI | GACGC | 1 cut(s) | 632 |
| Csp6I | GTAC | 3 cut(s) | 376, 997, 1182 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 889, 924 |
| CviAII | CATG | 8 cut(s) | 98, 145, 360, 500, 845, 969, 1046, 1202 |
| CviQI | GTAC | 3 cut(s) | 376, 997, 1182 |
| DdeI | CTNAG | 4 cut(s) | 53, 372, 624, 1408 |
| DpnI | GATC | 4 cut(s) | 440, 480, 804, 1011 |
| DpnII | GATC | 4 cut(s) | 438, 478, 802, 1009 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 642 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 642 |
| Eam1104I | CTCTTC | 2 cut(s) | 1320, 1397 |
| EarI | CTCTTC | 2 cut(s) | 1320, 1397 |
| Ecl136II | GAGCTC | 1 cut(s) | 1407 |
| Eco24I | GRGCYC | 2 cut(s) | 691, 1409 |
| Eco53kI | GAGCTC | 1 cut(s) | 1407 |
| Eco57I | CTGAAG | 2 cut(s) | 433, 791 |
| EcoICRI | GAGCTC | 1 cut(s) | 1407 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1302 |
| EcoRII | CCWGG | 2 cut(s) | 380, 548 |
| EcoT38I | GRGCYC | 2 cut(s) | 691, 1409 |
| Esp3I | CGTCTC | 2 cut(s) | 579, 650 |
| FaeI | CATG | 8 cut(s) | 101, 148, 363, 503, 848, 972, 1049, 1205 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 676, 708 |
| FatI | CATG | 8 cut(s) | 97, 144, 359, 499, 844, 968, 1045, 1201 |
| FauNDI | CATATG | 2 cut(s) | 32, 1030 |
| FbaI | TGATCA | 1 cut(s) | 478 |
| Fnu4HI | GCNGC | 3 cut(s) | 471, 571, 1111 |
| FokI | GGATG | 4 cut(s) | 255, 518, 999, 1368 |
| FriOI | GRGCYC | 2 cut(s) | 691, 1409 |
| Fsp4HI | GCNGC | 3 cut(s) | 471, 571, 1111 |
| FspBI | CTAG | 4 cut(s) | 776, 1035, 1052, 1175 |
| GlaI | GCGC | 3 cut(s) | 61, 184, 313 |
| GluI | GCNGC | 3 cut(s) | 471, 571, 1111 |
| GsuI | CTGGAG | 1 cut(s) | 142 |
| HgaI | GACGC | 1 cut(s) | 632 |
| HhaI | GCGC | 3 cut(s) | 62, 185, 314 |
| Hin1II | CATG | 8 cut(s) | 101, 148, 363, 503, 848, 972, 1049, 1205 |
| Hin6I | GCGC | 3 cut(s) | 60, 183, 312 |
| HinP1I | GCGC | 3 cut(s) | 60, 183, 312 |
| HincII | GTYRAC | 1 cut(s) | 1208 |
| HindII | GTYRAC | 1 cut(s) | 1208 |
| HinfI | GANTC | 5 cut(s) | 131, 422, 557, 848, 1088 |
| HphI | GGTGA | 3 cut(s) | 446, 650, 1210 |
| Hpy166II | GTNNAC | 3 cut(s) | 118, 672, 1208 |
| Hpy188I | TCNGA | 3 cut(s) | 427, 438, 977 |
| Hpy188III | TCNNGA | 1 cut(s) | 1092 |
| Hpy8I | GTNNAC | 3 cut(s) | 118, 672, 1208 |
| Hpy99I | CGWCG | 1 cut(s) | 1010 |
| HpyAV | CCTTC | 5 cut(s) | 702, 880, 1070, 1298, 1346 |
| HpyCH4III | ACNGT | 1 cut(s) | 506 |
| HpyCH4IV | ACGT | 2 cut(s) | 780, 855 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 285, 294, 356, 404, 1171 |
| HpyF3I | CTNAG | 4 cut(s) | 53, 372, 624, 1408 |
| HpySE526I | ACGT | 2 cut(s) | 780, 855 |
| Hsp92II | CATG | 8 cut(s) | 101, 148, 363, 503, 848, 972, 1049, 1205 |
| HspAI | GCGC | 3 cut(s) | 60, 183, 312 |
| Ksp22I | TGATCA | 1 cut(s) | 478 |
| Kzo9I | GATC | 4 cut(s) | 438, 478, 802, 1009 |
| LguI | GCTCTTC | 1 cut(s) | 1397 |
| LmnI | GCTCC | 2 cut(s) | 439, 694 |
| Lsp1109I | GCAGC | 2 cut(s) | 557, 1097 |
| LweI | GCATC | 7 cut(s) | 47, 112, 421, 540, 630, 935, 1186 |
| MaeI | CTAG | 4 cut(s) | 776, 1035, 1052, 1175 |
| MaeII | ACGT | 2 cut(s) | 780, 855 |
| MaeIII | GTNAC | 8 cut(s) | 35, 128, 452, 627, 728, 1121, 1216, 1366 |
| MalI | GATC | 4 cut(s) | 440, 480, 804, 1011 |
| MboI | GATC | 4 cut(s) | 438, 478, 802, 1009 |
| MboII | GAAGA | 7 cut(s) | 263, 398, 401, 797, 1265, 1337, 1414 |
| MfeI | CAATTG | 2 cut(s) | 67, 754 |
| MhlI | GDGCHC | 3 cut(s) | 625, 691, 1409 |
| MluCI | AATT | 5 cut(s) | 67, 238, 513, 544, 754 |
| MlyI | GAGTC | 2 cut(s) | 125, 842 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1269 |
| MseI | TTAA | 6 cut(s) | 230, 303, 495, 831, 840, 873 |
| MslI | CAYNNNNRTG | 1 cut(s) | 98 |
| MspA1I | CMGCKG | 2 cut(s) | 371, 1110 |
| MspR9I | CCNGG | 2 cut(s) | 382, 550 |
| MunI | CAATTG | 2 cut(s) | 67, 754 |
| Mva1269I | GAATGC | 3 cut(s) | 185, 297, 1146 |
| MvaI | CCWGG | 2 cut(s) | 382, 550 |
| MvnI | CGCG | 1 cut(s) | 312 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 285, 294, 356, 404, 1171 |
| NdeI | CATATG | 2 cut(s) | 32, 1030 |
| NdeII | GATC | 4 cut(s) | 438, 478, 802, 1009 |
| NlaIII | CATG | 8 cut(s) | 101, 148, 363, 503, 848, 972, 1049, 1205 |
| NlaIV | GGNNCC | 3 cut(s) | 245, 690, 913 |
| NmuCI | GTSAC | 5 cut(s) | 35, 128, 452, 1121, 1216 |
| NspI | RCATGY | 2 cut(s) | 148, 363 |
| OliI | CACNNNNGTG | 1 cut(s) | 98 |
| PaeI | GCATGC | 1 cut(s) | 363 |
| PciI | ACATGT | 1 cut(s) | 144 |
| PciSI | GCTCTTC | 1 cut(s) | 1397 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 640 |
| PctI | GAATGC | 3 cut(s) | 185, 297, 1146 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1269 |
| PfeI | GAWTC | 3 cut(s) | 422, 557, 1088 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 166 |
| PfoI | TCCNGGA | 1 cut(s) | 548 |
| PkrI | GCNGC | 3 cut(s) | 472, 572, 1112 |
| PleI | GAGTC | 2 cut(s) | 125, 842 |
| PpsI | GAGTC | 2 cut(s) | 125, 842 |
| PscI | ACATGT | 1 cut(s) | 144 |
| Psp124BI | GAGCTC | 1 cut(s) | 1409 |
| Psp6I | CCWGG | 2 cut(s) | 380, 548 |
| PspGI | CCWGG | 2 cut(s) | 380, 548 |
| PspN4I | GGNNCC | 3 cut(s) | 245, 690, 913 |
| PvuII | CAGCTG | 1 cut(s) | 371 |
| RsaI | GTAC | 3 cut(s) | 377, 998, 1183 |
| RsaNI | GTAC | 3 cut(s) | 376, 997, 1182 |
| RseI | CAYNNNNRTG | 1 cut(s) | 98 |
| SacI | GAGCTC | 1 cut(s) | 1409 |
| SapI | GCTCTTC | 1 cut(s) | 1397 |
| SaqAI | TTAA | 6 cut(s) | 230, 303, 495, 831, 840, 873 |
| SatI | GCNGC | 3 cut(s) | 471, 571, 1111 |
| Sau3AI | GATC | 4 cut(s) | 438, 478, 802, 1009 |
| ScaI | AGTACT | 2 cut(s) | 377, 1183 |
| SchI | GAGTC | 2 cut(s) | 125, 842 |
| ScrFI | CCNGG | 2 cut(s) | 382, 550 |
| SduI | GDGCHC | 3 cut(s) | 625, 691, 1409 |
| SfaNI | GCATC | 7 cut(s) | 47, 112, 421, 540, 630, 935, 1186 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 98 |
| SmlI | CTYRAG | 2 cut(s) | 682, 1019 |
| SmoI | CTYRAG | 2 cut(s) | 682, 1019 |
| SphI | GCATGC | 1 cut(s) | 363 |
| Sse9I | AATT | 5 cut(s) | 67, 238, 513, 544, 754 |
| SsiI | CCGC | 2 cut(s) | 471, 1108 |
| SspMI | CTAG | 4 cut(s) | 776, 1035, 1052, 1175 |
| SstI | GAGCTC | 1 cut(s) | 1409 |
| StyD4I | CCNGG | 2 cut(s) | 380, 548 |
| TaaI | ACNGT | 1 cut(s) | 506 |
| TaiI | ACGT | 2 cut(s) | 783, 858 |
| TaqI | TCGA | 3 cut(s) | 884, 1041, 1254 |
| TasI | AATT | 5 cut(s) | 67, 238, 513, 544, 754 |
| TatI | WGTACW | 2 cut(s) | 375, 1181 |
| TauI | GCSGC | 1 cut(s) | 473 |
| TfiI | GAWTC | 3 cut(s) | 422, 557, 1088 |
| Tru1I | TTAA | 6 cut(s) | 230, 303, 495, 831, 840, 873 |
| Tru9I | TTAA | 6 cut(s) | 230, 303, 495, 831, 840, 873 |
| TscAI | CASTG | 3 cut(s) | 481, 719, 815 |
| TseFI | GTSAC | 5 cut(s) | 35, 128, 452, 1121, 1216 |
| TseI | GCWGC | 2 cut(s) | 570, 1110 |
| Tsp45I | GTSAC | 5 cut(s) | 35, 128, 452, 1121, 1216 |
| TspDTI | ATGAA | 4 cut(s) | 161, 177, 345, 848 |
| TspRI | CASTG | 3 cut(s) | 481, 719, 815 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 166 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1302 |
| XapI | RAATTY | 1 cut(s) | 544 |
| XceI | RCATGY | 2 cut(s) | 148, 363 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1269 |
| XspI | CTAG | 4 cut(s) | 776, 1035, 1052, 1175 |
| ZrmI | AGTACT | 2 cut(s) | 377, 1183 |