Rh3DG273800

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0000226
Physical Location & Seq
Forward (+)
27634061 .. 27649761
15701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG273800.1

Sequence Viewer

Length: 4461 bp
ATGTTTAAGGAAGGGGAGCAGAAAAATGAAGATGATGCAGCAGCATCAGGTCCACCAGTTGTATGCTCTGCGTGTGGGGATCCAGTACTTGGTCCTAACTACAGTTGCAACCAGTGTTCCAAATTATTTATTCTCCACAAGTCATGTGCGGAGCTTCCCCGTGAGATGCACCACCCGATACACCGCAAACATCCACTTGTTCTCCTCCCAGATAGATATTACAACTGTGATATTTGTCGCAAATCCCATTCTGGCTTATTCTCTTACAAGTGTAAGCCATGTGACTTTGACCTTGACCTCCAGTGTGCTTCTAATTTGAGCGAAATAAAATACTTAGAGCACTTCAGTCATGAGCATGCACTGATCTTTGTGGGAGATCCAAAAAAACGAGAGGGAGATTTACCAGTTGTTTGTCAAGGGTGCGGAGAAAGAGTGTTAGGTCCAGGCTATTGCTGCAGCCGGTTTCTTTGTCACTACACTCTTCACAAGTCATGTGCGGAGCTTCCCCGTGACATGCACCACCCGATACACCGCAAACATCCACTTGTTCTCCTCCCAGATAGATATTACAACTGTGATATTTGTCGCAAATCCCATTCCGGCTTATTCTCTTACAAGTGTAAGCCATGTGACTTTGACCTTGACCTCCAGTGTGCTTCTAATTTGAGCGAAATAAAATACTTAGAGCACTTCAGTCATGAGCATGCACTGATCTTTGTAGAAAATCCAGAAAAACGAGAGGGAGATTTACCAGTTATTTGTCAAGGGTGCGGAGAACGAGTGTTAGGTCCAGGCTATTGCTGCAGTCGGTTTCTCTGTCACTACACTCTCCATAAGTCATGTGCGGAGCTTCCCCGTGAGATGCACCACCCGATACACCACAAACATCCACTTGTTCTCCTCCCAGATAGATATTACAGCTGTGATATTTGTCGCAAATCCCATTCCGGCTTATTCTCCTACAATTGTAAGCCATGTGACTTCGACCTTGACCTCCAGTGTGCTTCTAATTTGAGCGAAATAAAATACTTAGAGCACTTCAGTCATGAGCATGCACTGATCTTTGTGGAAGATCCGAAAAGACGAGAGGGAGGTTTACCAGTTGTTTGTCAAGGGTGCCAGGAGGAAGTGTTGGCTCCAAGCTATTGCTGTAGTCGGATTCTTTGCCACTACACTCTCCATAAGTCATGTGCGGAGATGTCGGTTGAGTTTCATCACCCAAGGCATATCCAACACCCGCTTATTCTCAACAAATTAGATGCAGCAAAACAACACCTTTGTGATGCTTGTGACCAAGATTGCAGCGGCAAGTTCACTTATAGTTGCTTCCAGTGTCACTTCAACCTTGACCCTCAATGTACTTTCAATTGGACTGACTTTAAACATTTCAGTCACGTCCATCCACTGATGTTTAAGGAAGGGGAGCAGGAAATGTCTGACCGAGGATCTATATCTCCCAGCAACTCCTTTGAGATCGACGTTGGAGAAGTAGAAGTAGAAACACCACCAGAGTCTATTCTGGGTGTCTCTTCCTATCTCGAAGACAATGGACACTCGGAAGACATGGCTGTGGGGAAAGAAGAGATATTTAAAGCCCGTCCGGACAAGAGGGAGTACAGGAGGATCGTCCTCCCTAACTCCCTTGAAGTCCTTCTCATCAGCGATCCTGATACTGACAAGTGTGCTGCTTCCATGGATGTCAGTGTTGGTTCTTTCAGCGACCCTGATGGCCTGGAGGGCCTTGCCCATTTCCTTGAGCATATGCTGTTTTATGCTAGTGAGAAGCACCCTTTGGAGGATACTTACTCGAAGTACATCACTGAGCATGGAGGAAGAACAAATGCTTTCACAGCTGCAGAACACACCAACTTTTATTTTGACATTAATCCCGATGGTTTTGAAGAGGCTCTGGACAGGTTTGCTCAGTTCTTTATTAAACCCTTGATGTCAGCTGATGCTACAACGAGGGAAATTAAAGCTGTTGATTCAGAAAATCAGAAAAATCTGTTGTCCGATGGCTGGAGAATAAACCAGCTTCAGAAGCATCTAAGTGCAGCGAATCATCCTTACCATAAATTTAGTACAGGCAATTGGAATACTTTGGAGGTTCAACCAAAAGCAAAAGGGTTGGATACCAGACATGAACTTATTAAATTCTATGAAGAATACTATTCAGCCAACCTCATGCATCTGGTTATATACGGAAAAGAGAAACTTGATAAAATTCAAGGCCTTGTAGAGGACAAGTTTAAGGAAATTCGAAACATTGACCGAAGTTGCCTTCGTTTTACTGGTGAGCCTTGCACATCAGAACATCTGGAGATTCTTGTCAGAACCGTTCCAATAAAAGAGGGTCATAAATTGAGACTTGCATGGCCTATAACTCCAGAAATTCACCATTACAAGGAAGGGCCATGTAGGTATCTTGGTCATCTTATCGGCCATGAAGGAGAAGGATCTTTATATTACATCTTGAAAACATTGGGATGGGCAACAGGTTTGTCGGCTGGTGAATCAGATTCTACTTTGGACTTTGCTTTCTTTAAAGTAGATATTGATCTCACCGATGCAGGCCACGACCACATGCAAGATACAGTAGGCTTGCTTTTCAAATACATTTCCCTGTTACAGCAGTCGGGTGTCTGCAAATGGATATTTGATGAGCTGTCTGCTGTTTGTGAGACAAAGTTTCATTATCAGGACAAAATTCAGCCTATTAATTACGTGGTCAATATTTCATCAAATATGCAGAAATATCCCCCGAAAGACTGGCTTGTAAGATCATCATTGCCTTCTAACTTCAGTCCAGTCATTATCCAAATGGTGCTGAACAAGCTTTCTCCAAACAATGTCCGAATCTTCTGGGAGTCCAAGAAATTTGAAGGTGATACAAACATGTTTGAGCCCTGGTATGGAACTGCTTACTGCATGGAGAAAATTACGGGCTCCAGAATTCAGGAATGGATAGCATCTTCCCCAAATGAGAATTTACACCTACCAGCACGTAATGTTTTCATCCCCACGGACTTGTCTCTTAAGAATGAGAATGAGAAGGTCAAATGTCCAGTTCTATTAAGAAAGTCACCATATGCAACACTATGGTACAAGCCTGATACAATGTTTTTTACTCCTAAAGCATACGTCAAGATTGATTTCAATTGTCCCCATGCAAGTGGCTCCCCTGAAGCAGAAGTCCTGACTAATATTTTTACTCACTTGTTAATGGATTACTTGAATGACTATGCTTATTATGCGAAGGTTGCTGGACTGTACTATGGAATAAACCACACAGAAAGTGGTTTTCAGGTGACTCTGGTTGGTTATAATCACAAATTGAGGATTTTACTGGAAACGGTAGTTGAGAAGATGGCAAGCTTTAAAGTGAAAGCTGACAGGTTCTCTGTGATCAAGGAAATGGTCACAAAGAAGTACCAAAATTACAAATTTCAACAGCCCTATGAGCAGGCTATGTACTATTTCTCATTAATTCTACAGTACGAGACATGGCCATTGATGGAACAACTAGAAGTTCTTCCTCATCTTGAAGTTGAAGATCTTGCTAAGTTTGTTCCATTGATGCTCTCAAGGGCCTTCTTAGAGTGTTACACAGCAGGAAACCTTGAAAGTACTGAAGCTGAGTCAATGATCCTGCACGTAGAAGATGTTTTTTTTAAGGGTTCAAACCCTATTTGCCAACCTTTATTCCCATCCCAACATTTGACAAATAGAGTCGTGAAGCTTGAAAAGGGAAAAAACTTTATCTATCCTGTGGAAGGCCTTAATCCAAGTGATGAGAATTCTTCCCTTGTCCACTATATCCAGGTTCATCGAGATGATTTTATGCTGAATGTGAAACTTCGGCTGTTTGTTCTTATTGCAAAGCAACCAGCCTTTCACCAACTTAGATCAGTCGAGCAACTTGGTTACATAACTCGTCTTTCACAGAGGAATGATTGTGGTATCCGCGGACTGCAGTTTATTATACAATCCACAGTGAAGGGTCCAGGACATATTGATTTGAGAGTGGAGGAATTTCTCAAAACGTTTGAGAGTAAGCTTTATGAGATGACAAATGACGAATTCAAGAGCAATGTAAATACTTTGATCGATATGAAGCTTGAGAAGCACAAGAACTTGAGGGAAGAAGCTGGATTTTATTGGAGGGAGATTTCTGATGGGACCCTCAAGTTTGATCGTAAAGAAGCAGAGATTGCAGCACTGAGGCAACTTACACAACAAGAGTTGATTGAGTTTTATAATGAGCATATAAAAGTTGGGGCACCTCGTAAGAGGACATTGAGTGTACGAGTATATGGGAATCCTCATTCATCTGAGTATGCATCAGATAAAAGCTCCCCAGTGCAACCTTGCACTGTCAAAATCGATGATATATTCACTTTCAGAAGGTCACAGCCTCTTTATGGTTCATTCAAGGGAAACTTTGGTCATGTGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

1486

Amino Acids

171.0

Weight (kDa)

6.16

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C1_2 PF03107 60 - 103 2.4e-08 C1 domain
C1_2 PF03107 115 - 166 2.8e-06 C1 domain
C1_2 PF03107 176 - 219 2.4e-08 C1 domain
C1_2 PF03107 231 - 282 6.3e-06 C1 domain
C1_2 PF03107 293 - 335 2.8e-08 C1 domain
C1_2 PF03107 407 - 453 2.7e-06 C1 domain
Peptidase_M16 PF00675 550 - 684 2.6e-35 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 712 - 889 2.2e-29 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 897 - 1176 1.1e-102 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 1181 - 1367 5.8e-11 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 1292 - 1387 1.2e-20 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000226)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G58037 AT1G58037 AT1G60787 AT1G60787 AT1G60787 AT1G60787 AT1G65180 AT1G69150 AT2G04500 AT2G04680 AT2G19650 AT2G19660 AT2G19660 AT2G21810 AT2G21830 AT2G21830 AT2G21840 AT2G21850 AT2G43220 AT3G06990 AT3G07000 AT3G25850 AT3G27473 AT3G27473 AT3G27473 AT3G27473 AT3G27480 AT3G27480 AT3G27490 AT3G27490 AT3G27500 AT3G27500 AT3G27500 AT3G27510 AT3G27510 AT3G28650 AT3G43890 AT3G45530 AT3G45840 AT3G46800 AT3G46810 AT3G48400 AT4G01350 AT4G01740 AT4G01760 AT4G01910 AT4G01920 AT4G01925 AT4G01930 AT4G02180 AT4G02190 AT4G09690 AT4G11390 AT4G11390 AT4G11540 AT4G11550 AT4G13992 AT4G14980 AT4G16015 AT4G16015 AT5G22355 AT5G26190 AT5G29624 AT5G37620 AT5G39471 AT5G40320 AT5G42840 AT5G42840 AT5G43030 AT5G43040 AT5G44770 AT5G45730 AT5G54020 AT5G54020 AT5G54030 AT5G54040 AT5G54050 AT5G59920 AT5G59930 AT5G59930 AT5G59940
fragaria_vesca FvH4_4g12791 FvH4_6g40980 FvH4_6g41040 FvH4_6g47450 FvH4_7g18770
malus_domestica MD00G1084300.v1.1 MD12G1059900.v1.1 MD14G1057000.v1.1 MD14G1057200.v1.1 MD14G1060000.v1.1 MD14G1060100.v1.1
prunus_persica Prupe.7G074300_v2.0.a1 Prupe.7G074400_v2.0.a1 Prupe.7G074600_v2.0.a1 Prupe.7G074600_v2.0.a1 Prupe.7G078300_v2.0.a1 Prupe.7G078400_v2.0.a1 Prupe.7G078800_v2.0.a1 Prupe.7G081900_v2.0.a1 Prupe.7G092300_v2.0.a1
pyrus_communis pycom14g04870
rosa_chinensis RchiOBHm_Chr1g0362581 RchiOBHm_Chr3g0480191
rosa_laevigata RLG00000023512 RLG00000023513 RLG00000027637
rosa_multiflora Rmu_co8180116.1_g000001 Rmu_co8226133.1_g000001 Rmu_sc0005725.1_g000009 Rmu_sc0006604.1_g000012 Rmu_sc0015624.1_g000001 Rmu_sc0020879.1_g000005 Rmu_sc0020879.1_g000008
rosa_roxburghii Rroxscaffold_4G00293940 Rroxscaffold_6G00401730
rosa_rugosa Rorug01G0299800.1 Rorug03G0180900 Rorug04G0106000 Rorug04G0106100
rosa_samantha Rh1AG308400 Rh1BG271700 Rh1BG271800 Rh1BG272000 Rh1BG272100 Rh1CG289300 Rh1DG302100 Rh1DG302200 Rh1DG302400 Rh1DG302500 Rh3BG264400 Rh3CG262400 Rh3CG262500 Rh3DG258100 Rh3DG258200 Rh3DG258300 Rh3DG273800 Rh4BG170600 Rh7AG404100 Rh7AG404700 Rh7CG422900
rosa_wichuraiana Rw0G000030 Rw1G027380 Rw1G027390 Rw3G020880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3324, 4257
AccB1I GGYRCC 2 cut(s) 1116, 4279
AccB7I CCANNNNNTGG 1 cut(s) 89
AccII CGCG 1 cut(s) 3966
AccIII TCCGGA 1 cut(s) 1600
AclI AACGTT 1 cut(s) 4043
AclWI GGATC 9 cut(s) 74, 87, 371, 1067, 1453, 1631, 1658, 2464, 3640
AcoI YGGCCR 2 cut(s) 2440, 3506
AcuI CTGAAG 7 cut(s) 328, 676, 1024, 2021, 2785, 3204, 3651
AfiI CCNNNNNNNGG 8 cut(s) 89, 1795, 2019, 2239, 2404, 2912, 3773, 4421
AflII CTTAAG 1 cut(s) 3035
AflIII ACRYGT 1 cut(s) 2895
AjiI CACGTC 1 cut(s) 1396
AjnI CCWGG 7 cut(s) 442, 790, 1119, 1731, 2906, 3819, 4003
AjuI GAANNNNNNNTTGG 2 cut(s) 1775, 1807
AleI CACNNNNGTG 1 cut(s) 1278
Alw21I GWGCWC 3 cut(s) 342, 690, 1038
Alw26I GTCTC 5 cut(s) 1531, 2359, 2675, 3036, 3494
AlwI GGATC 9 cut(s) 74, 87, 371, 1067, 1453, 1631, 1658, 2464, 3640
Aor13HI TCCGGA 1 cut(s) 1600
AseI ATTAAT 3 cut(s) 1884, 2718, 3485
Asp700I GAANNNNTTC 2 cut(s) 1650, 3531
AspS9I GGNCC 9 cut(s) 50, 92, 440, 788, 1738, 2411, 3588, 4001, 4179
AsuHPI GGTGA 9 cut(s) 1208, 2306, 2387, 2522, 2554, 2897, 3075, 3319, 3887
AsuII TTCGAA 1 cut(s) 2260
AvaII GGWCC 6 cut(s) 50, 92, 440, 788, 4001, 4179
BaeGI GKGCMC 1 cut(s) 4282
BalI TGGCCA 1 cut(s) 3508
BamHI GGATCC 1 cut(s) 79
BanI GGYRCC 2 cut(s) 1116, 4279
BanII GRGCYC 2 cut(s) 2907, 2948
BbsI GAAGAC 2 cut(s) 1548, 1566
Bbv12I GWGCWC 3 cut(s) 342, 690, 1038
BccI CCATC 9 cut(s) 1407, 1721, 1886, 2009, 2481, 3361, 3508, 3715, 4169
BciT130I CCWGG 7 cut(s) 444, 792, 1121, 1733, 2908, 3821, 4005
BciVI GTATCC 3 cut(s) 1792, 2125, 3971
BclI TGATCA 1 cut(s) 3405
BcoDI GTCTC 5 cut(s) 1531, 2359, 2675, 3036, 3494
BfaI CTAG 2 cut(s) 1776, 3524
BfmI CTRYAG 7 cut(s) 100, 454, 802, 1150, 1854, 3491, 3971
BfrI CTTAAG 1 cut(s) 3035
BfuI GTATCC 3 cut(s) 1792, 2125, 3971
BglI GCCNNNNNGGC 1 cut(s) 1737
BglII AGATCT 1 cut(s) 3553
BmcAI AGTACT 2 cut(s) 87, 3628
Bme1390I CCNGG 7 cut(s) 444, 792, 1121, 1733, 2908, 3821, 4005
Bme18I GGWCC 6 cut(s) 50, 92, 440, 788, 4001, 4179
BmgBI CACGTC 1 cut(s) 1396
BmgT120I GGNCC 9 cut(s) 50, 92, 440, 788, 1738, 2411, 3588, 4001, 4179
BmiI GGNNCC 9 cut(s) 81, 1118, 1137, 2947, 3178, 4002, 4180, 4181, 4281
BmrFI CCNGG 7 cut(s) 444, 792, 1121, 1733, 2908, 3821, 4005
BmrI ACTGGG 1 cut(s) 4352
BmuI ACTGGG 1 cut(s) 4352
BpiI GAAGAC 2 cut(s) 1548, 1566
BpmI CTGGAG 8 cut(s) 284, 632, 980, 1754, 2041, 2339, 2370, 2932
Bpu14I TTCGAA 1 cut(s) 2260
BpuEI CTTGAG 5 cut(s) 1775, 3568, 4139, 4156, 4169
Bsa29I ATCGAT 2 cut(s) 4107, 4383
BsaAI YACGTR 3 cut(s) 2725, 3005, 3655
BsaBI GATNNNNATC 2 cut(s) 1450, 2556
BsaJI CCNNGG 6 cut(s) 1220, 1441, 1692, 2906, 3021, 3964
BsaWI WCCGGW 1 cut(s) 1600
Bsc4I CCNNNNNNNGG 8 cut(s) 89, 1795, 2019, 2239, 2404, 2912, 3773, 4421
Bse118I RCCGGY 1 cut(s) 459
Bse3DI GCAATG 2 cut(s) 2786, 4096
Bse8I GATNNNNATC 2 cut(s) 1450, 2556
BseAI TCCGGA 1 cut(s) 1600
BseBI CCWGG 7 cut(s) 444, 792, 1121, 1733, 2908, 3821, 4005
BseCI ATCGAT 2 cut(s) 4107, 4383
BseDI CCNNGG 6 cut(s) 1220, 1441, 1692, 2906, 3021, 3964
BseGI GGATG 9 cut(s) 190, 538, 886, 1399, 1702, 2062, 2492, 3015, 3707
BseJI GATNNNNATC 2 cut(s) 1450, 2556
BseLI CCNNNNNNNGG 8 cut(s) 89, 1795, 2019, 2239, 2404, 2912, 3773, 4421
BseMI GCAATG 2 cut(s) 2786, 4096
BseMII CTCAG 5 cut(s) 1812, 1937, 3627, 4211, 4323
BseRI GAGGAG 3 cut(s) 194, 542, 890
BseSI GKGCMC 1 cut(s) 4282
BseYI CCCAGC 1 cut(s) 1457
BsgI GTGCAG 2 cut(s) 2073, 3635
Bsh1236I CGCG 1 cut(s) 3966
BshNI GGYRCC 2 cut(s) 1116, 4279
BshVI ATCGAT 2 cut(s) 4107, 4383
BsiHKAI GWGCWC 3 cut(s) 342, 690, 1038
BsiSI CCGG 4 cut(s) 460, 600, 948, 1601
BslFI GGGAC 2 cut(s) 3147, 4192
BslI CCNNNNNNNGG 8 cut(s) 89, 1795, 2019, 2239, 2404, 2912, 3773, 4421
BsmAI GTCTC 5 cut(s) 1531, 2359, 2675, 3036, 3494
BsmFI GGGAC 2 cut(s) 3147, 4192
Bsp119I TTCGAA 1 cut(s) 2260
Bsp1286I GDGCHC 6 cut(s) 342, 690, 1038, 2907, 2948, 4282
Bsp13I TCCGGA 1 cut(s) 1600
Bsp19I CCATGG 1 cut(s) 1692
BspCNI CTCAG 5 cut(s) 1813, 1936, 3628, 4212, 4324
BspDI ATCGAT 2 cut(s) 4107, 4383
BspEI TCCGGA 1 cut(s) 1600
BspFNI CGCG 1 cut(s) 3966
BspHI TCATGA 3 cut(s) 349, 697, 1045
BspLI GGNNCC 9 cut(s) 81, 1118, 1137, 2947, 3178, 4002, 4180, 4181, 4281
BspMAI CTGCAG 4 cut(s) 458, 806, 1858, 3975
BspPI GGATC 9 cut(s) 74, 87, 371, 1067, 1453, 1631, 1658, 2464, 3640
BspT104I TTCGAA 1 cut(s) 2260
BspT107I GGYRCC 2 cut(s) 1116, 4279
BspTI CTTAAG 1 cut(s) 3035
BsrDI GCAATG 2 cut(s) 2786, 4096
BsrFI RCCGGY 1 cut(s) 459
BssAI RCCGGY 1 cut(s) 459
BssECI CCNNGG 6 cut(s) 1220, 1441, 1692, 2906, 3021, 3964
BssT1I CCWWGG 2 cut(s) 1220, 1692
Bst2UI CCWGG 7 cut(s) 444, 792, 1121, 1733, 2908, 3821, 4005
Bst6I CTCTTC 4 cut(s) 486, 1534, 1575, 1896
BstAFI CTTAAG 1 cut(s) 3035
BstAPI GCANNNNNTGC 1 cut(s) 4211
BstBAI YACGTR 3 cut(s) 2725, 3005, 3655
BstBI TTCGAA 1 cut(s) 2260
BstC8I GCNNGC 7 cut(s) 357, 705, 1053, 2572, 2603, 3373, 3465
BstDSI CCRYGG 3 cut(s) 1692, 3021, 3964
BstENI CCTNNNNNAGG 2 cut(s) 2237, 3771
BstF5I GGATG 9 cut(s) 190, 538, 886, 1399, 1702, 2062, 2492, 3015, 3707
BstFNI CGCG 1 cut(s) 3966
BstMAI GTCTC 5 cut(s) 1531, 2359, 2675, 3036, 3494
BstNI CCWGG 7 cut(s) 444, 792, 1121, 1733, 2908, 3821, 4005
BstNSI RCATGY 6 cut(s) 359, 517, 707, 1055, 2587, 2899
BstSCI CCNGG 7 cut(s) 442, 790, 1119, 1731, 2906, 3819, 4003
BstSFI CTRYAG 7 cut(s) 100, 454, 802, 1150, 1854, 3491, 3971
BstSLI GKGCMC 1 cut(s) 4282
BstUI CGCG 1 cut(s) 3966
BstV2I GAAGAC 2 cut(s) 1548, 1566
BstX2I RGATCY 6 cut(s) 79, 376, 1072, 1445, 2456, 3553
BstXI CCANNNNNNTGG 1 cut(s) 3173
BstYI RGATCY 6 cut(s) 79, 376, 1072, 1445, 2456, 3553
Bsu15I ATCGAT 2 cut(s) 4107, 4383
BsuI GTATCC 3 cut(s) 1792, 2125, 3971
BsuTUI ATCGAT 2 cut(s) 4107, 4383
BtgI CCRYGG 3 cut(s) 1692, 3021, 3964
BtrI CACGTC 1 cut(s) 1396
BtsCI GGATG 9 cut(s) 190, 538, 886, 1399, 1702, 2062, 2492, 3015, 3707
Cac8I GCNNGC 7 cut(s) 357, 705, 1053, 2572, 2603, 3373, 3465
CciI TCATGA 3 cut(s) 349, 697, 1045
Cfr10I RCCGGY 1 cut(s) 459
Cfr13I GGNCC 9 cut(s) 50, 92, 440, 788, 1738, 2411, 3588, 4001, 4179
Cfr42I CCGCGG 1 cut(s) 3967
ClaI ATCGAT 2 cut(s) 4107, 4383
DraI TTTAAA 4 cut(s) 1381, 1591, 2545, 3379
EaeI YGGCCR 2 cut(s) 2440, 3506
Eam1104I CTCTTC 4 cut(s) 486, 1534, 1575, 1896
EarI CTCTTC 4 cut(s) 486, 1534, 1575, 1896
Eco130I CCWWGG 2 cut(s) 1220, 1692
Eco147I AGGCCT 2 cut(s) 2232, 3777
Eco24I GRGCYC 2 cut(s) 2907, 2948
Eco47I GGWCC 6 cut(s) 50, 92, 440, 788, 4001, 4179
Eco57I CTGAAG 7 cut(s) 328, 676, 1024, 2021, 2785, 3204, 3651
EcoNI CCTNNNNNAGG 2 cut(s) 2237, 3771
EcoO109I RGGNCCY 3 cut(s) 1738, 3588, 4179
EcoRI GAATTC 3 cut(s) 2952, 3796, 4079
EcoRII CCWGG 7 cut(s) 442, 790, 1119, 1731, 2906, 3819, 4003
EcoT14I CCWWGG 2 cut(s) 1220, 1692
EcoT22I ATGCAT 2 cut(s) 2190, 4342
EcoT38I GRGCYC 2 cut(s) 2907, 2948
ErhI CCWWGG 2 cut(s) 1220, 1692
FalI AAGNNNNNCTT 6 cut(s) 2199, 2231, 2757, 2789, 4424, 4456
FaqI GGGAC 2 cut(s) 3147, 4192
FauI CCCGC 1 cut(s) 1245
FauNDI CATATG 2 cut(s) 1761, 3088
FbaI TGATCA 1 cut(s) 3405
FokI GGATG 9 cut(s) 177, 525, 873, 1386, 1709, 2049, 2499, 3002, 3694
FriOI GRGCYC 2 cut(s) 2907, 2948
FspBI CTAG 2 cut(s) 1776, 3524
GsaI CCCAGC 1 cut(s) 1461
GsuI CTGGAG 8 cut(s) 284, 632, 980, 1754, 2041, 2339, 2370, 2932
HapII CCGG 4 cut(s) 460, 600, 948, 1601
HindIII AAGCTT 5 cut(s) 2834, 3373, 3737, 4055, 4115
HpaII CCGG 4 cut(s) 460, 600, 948, 1601
HphI GGTGA 9 cut(s) 1208, 2306, 2387, 2522, 2554, 2897, 3075, 3319, 3887
Hpy166II GTNNAC 5 cut(s) 53, 1097, 1314, 3811, 4304
Hpy8I GTNNAC 5 cut(s) 53, 1097, 1314, 3811, 4304
Hpy99I CGWCG 1 cut(s) 1481
HpyCH4IV ACGT 7 cut(s) 1395, 1479, 2724, 3004, 3141, 3654, 4043
HpySE526I ACGT 7 cut(s) 1395, 1479, 2724, 3004, 3141, 3654, 4043
KflI GGGWCCC 1 cut(s) 4179
Kpn2I TCCGGA 1 cut(s) 1600
Ksp22I TGATCA 1 cut(s) 3405
KspI CCGCGG 1 cut(s) 3967
LmnI GCTCC 9 cut(s) 16, 151, 499, 847, 1141, 1423, 2951, 3182, 4358
MaeI CTAG 2 cut(s) 1776, 3524
MaeII ACGT 7 cut(s) 1395, 1479, 2724, 3004, 3141, 3654, 4043
MfeI CAATTG 4 cut(s) 964, 1366, 2089, 3157
MflI RGATCY 6 cut(s) 79, 376, 1072, 1445, 2456, 3553
MhlI GDGCHC 6 cut(s) 342, 690, 1038, 2907, 2948, 4282
MlsI TGGCCA 1 cut(s) 3508
MluNI TGGCCA 1 cut(s) 3508
MlyI GAGTC 5 cut(s) 1520, 2876, 3304, 3647, 3738
MmeI TCCRAC 4 cut(s) 1136, 1255, 1462, 2109
Mox20I TGGCCA 1 cut(s) 3508
Mph1103I ATGCAT 2 cut(s) 2190, 4342
MroI TCCGGA 1 cut(s) 1600
MroXI GAANNNNTTC 2 cut(s) 1650, 3531
MscI TGGCCA 1 cut(s) 3508
MslI CAYNNNNRTG 9 cut(s) 354, 702, 1050, 1278, 1568, 2049, 2485, 3171, 3831
Msp20I TGGCCA 1 cut(s) 3508
MspA1I CMGCKG 5 cut(s) 921, 1305, 1853, 1952, 3966
MspCI CTTAAG 1 cut(s) 3035
MspI CCGG 4 cut(s) 460, 600, 948, 1601
MspR9I CCNGG 7 cut(s) 444, 792, 1121, 1733, 2908, 3821, 4005
MunI CAATTG 4 cut(s) 964, 1366, 2089, 3157
MvaI CCWGG 7 cut(s) 444, 792, 1121, 1733, 2908, 3821, 4005
MvnI CGCG 1 cut(s) 3966
NcoI CCATGG 1 cut(s) 1692
NdeI CATATG 2 cut(s) 1761, 3088
NlaIV GGNNCC 9 cut(s) 81, 1118, 1137, 2947, 3178, 4002, 4180, 4181, 4281
NsiI ATGCAT 2 cut(s) 2190, 4342
NspI RCATGY 6 cut(s) 359, 517, 707, 1055, 2587, 2899
NspV TTCGAA 1 cut(s) 2260
OliI CACNNNNGTG 1 cut(s) 1278
PaeI GCATGC 3 cut(s) 359, 707, 1055
PagI TCATGA 3 cut(s) 349, 697, 1045
PceI AGGCCT 2 cut(s) 2232, 3777
PciI ACATGT 1 cut(s) 2895
PdmI GAANNNNTTC 2 cut(s) 1650, 3531
PfeI GAWTC 8 cut(s) 1159, 1985, 2059, 2323, 2513, 2519, 2856, 4318
PflMI CCANNNNNTGG 1 cut(s) 89
PfoI TCCNGGA 1 cut(s) 4003
PleI GAGTC 5 cut(s) 1519, 2875, 3304, 3646, 3737
PpsI GAGTC 5 cut(s) 1519, 2875, 3304, 3646, 3737
Ppu21I YACGTR 3 cut(s) 2725, 3005, 3655
PpuMI RGGWCCY 1 cut(s) 4179
PscI ACATGT 1 cut(s) 2895
PshBI ATTAAT 3 cut(s) 1884, 2718, 3485
PsiI TTATAA 2 cut(s) 3324, 4257
Psp1406I AACGTT 1 cut(s) 4043
Psp5II RGGWCCY 1 cut(s) 4179
Psp6I CCWGG 7 cut(s) 442, 790, 1119, 1731, 2906, 3819, 4003
PspFI CCCAGC 1 cut(s) 1457
PspGI CCWGG 7 cut(s) 442, 790, 1119, 1731, 2906, 3819, 4003
PspN4I GGNNCC 9 cut(s) 81, 1118, 1137, 2947, 3178, 4002, 4180, 4181, 4281
PspPI GGNCC 9 cut(s) 50, 92, 440, 788, 1738, 2411, 3588, 4001, 4179
PspPPI RGGWCCY 1 cut(s) 4179
PstI CTGCAG 4 cut(s) 458, 806, 1858, 3975
PsuI RGATCY 6 cut(s) 79, 376, 1072, 1445, 2456, 3553
PvuII CAGCTG 3 cut(s) 921, 1853, 1952
RseI CAYNNNNRTG 9 cut(s) 354, 702, 1050, 1278, 1568, 2049, 2485, 3171, 3831
SacII CCGCGG 1 cut(s) 3967
Sau96I GGNCC 9 cut(s) 50, 92, 440, 788, 1738, 2411, 3588, 4001, 4179
ScaI AGTACT 2 cut(s) 87, 3628
SchI GAGTC 5 cut(s) 1520, 2876, 3304, 3647, 3738
ScrFI CCNGG 7 cut(s) 444, 792, 1121, 1733, 2908, 3821, 4005
SduI GDGCHC 6 cut(s) 342, 690, 1038, 2907, 2948, 4282
SfcI CTRYAG 7 cut(s) 100, 454, 802, 1150, 1854, 3491, 3971
SfiI GGCCNNNNNGGCC 1 cut(s) 1737
Sfr303I CCGCGG 1 cut(s) 3967
SfuI TTCGAA 1 cut(s) 2260
SgrBI CCGCGG 1 cut(s) 3967
SinI GGWCC 6 cut(s) 50, 92, 440, 788, 4001, 4179
SmiMI CAYNNNNRTG 9 cut(s) 354, 702, 1050, 1278, 1568, 2049, 2485, 3171, 3831
SmlI CTYRAG 6 cut(s) 1754, 3035, 3583, 4118, 4135, 4184
SmoI CTYRAG 6 cut(s) 1754, 3035, 3583, 4118, 4135, 4184
SphI GCATGC 3 cut(s) 359, 707, 1055
SseBI AGGCCT 2 cut(s) 2232, 3777
SspI AATATT 2 cut(s) 2734, 3205
SspMI CTAG 2 cut(s) 1776, 3524
StuI AGGCCT 2 cut(s) 2232, 3777
StyD4I CCNGG 7 cut(s) 442, 790, 1119, 1731, 2906, 3819, 4003
StyI CCWWGG 2 cut(s) 1220, 1692
TaiI ACGT 7 cut(s) 1398, 1482, 2727, 3007, 3144, 3657, 4046
TaqI TCGA 9 cut(s) 984, 1476, 1539, 1808, 2260, 3829, 3912, 4107, 4383
TaqII GACCGA 2 cut(s) 1455, 2286
TatI WGTACW 8 cut(s) 85, 1358, 1614, 1812, 2081, 3270, 3471, 3626
TauI GCSGC 1 cut(s) 1308
TfiI GAWTC 8 cut(s) 1159, 1985, 2059, 2323, 2513, 2519, 2856, 4318
TspGWI ACGGA 2 cut(s) 2217, 3038
Van91I CCANNNNNTGG 1 cut(s) 89
Vha464I CTTAAG 1 cut(s) 3035
VpaK11BI GGWCC 6 cut(s) 50, 92, 440, 788, 4001, 4179
VspI ATTAAT 3 cut(s) 1884, 2718, 3485
XagI CCTNNNNNAGG 2 cut(s) 2237, 3771
XceI RCATGY 6 cut(s) 359, 517, 707, 1055, 2587, 2899
XcmI CCANNNNNNNNNTGG 1 cut(s) 3293
XmnI GAANNNNTTC 2 cut(s) 1650, 3531
XspI CTAG 2 cut(s) 1776, 3524
ZrmI AGTACT 2 cut(s) 87, 3628
Zsp2I ATGCAT 2 cut(s) 2190, 4342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.