Rorug04G0106100

C1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000226
Physical Location & Seq
Forward (+)
16412642 .. 16417385
4744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0106100.1

Sequence Viewer

Length: 2466 bp
ATGAAACCAATCATCAGTCCTCTCTACCTCTACTTGTTCCTCCACATCCTCACTCTCTTCGTCACCGGTCAGTCGCCACCATACATCCCGGTCGAAAATATCACCCTTGCCTGTGGCAATTCCGGCCAACTAACCTCCAATGACAACAGAAAGTGGAGTGGAGATATCGACTCAAAATTCACCCCAATTGGTAACGCATCCGAATCCATAAATGCACCGCGGTCGCACACCGGTAGCCGAGTCCCTTACTCCAAAGCTCGGCTCTCTCGCTCCAACTTCACTTACACAATTCCCCTCTCTCCAGGCCAAAAATTCATCCGCTTGCATTTCAATCCGGATACATACACCCCGCACTTCCAACGCCCCAACTCCCTCTTCGCCGTCACCGCCGGCGGCTTCACTCTTCTCAAAGACTTCAACGCCTCCTTCACCGCCGACGCTTTCGGCGAGTCCCCGCTGGTCAGAGAATTCTGTGTAAACATCGAGACAGGACGGAGACTCGAGATCACCTTCACACCAAGCAGAGATGCGTACGCTTTCATCAACGGAATCGAAATCGTGTCCATGCCCACCGGTCTTTACAATACTTCTTCAGCTAACATGGTGGACGCCAATGCAGGCTCATTTGCGATCGGAAACAAAACAGCTCTGGAGATGGTGTACCGACTCAACGTCGGCGGAGGCCCCATCTGGCCGAACGCAGACACCGGCATGTACCGGACTTGGGAAACTGCCGACGATATTTATTTGGATAATGTAAGTAAAAAACTCAGCGTGCTACAAAACGATACAATCGAGCTCAACTTCGTCGAAGTTCCAGAATACTCTGCTCCAAAACCAGTTTACACAACAGGTCGGTCAATGGGTTGGGACGTAACCACCGGCAATAACCTCACATGGGTATTTCCCTTAGATCCCAACTTTTATTACCTGGTGAGACTCCATTTCTGTGGGATTCGAAACAGAGAGCGATGGTTTCAAATCTACTTGGCCAATCAAACCGCAGATGTCATCCGTTGGAGTGGAGGAGATGGGATTCCAGTTTACAAAGACTACGTTGTGGGCATGTTCATGCCTCCGGTCGGAAGCCAGAAAAAAGTTGACTTATTTCTCGCACTTTCACCGCTCCCGATTGGGTATGCTGACAGCAATGCCATGTTGAACGGGCTGGAAATCTTCAAGCTCAGTGACTCGAGTGGGAATTTCGCCGGACCCAACCCCAATCCACCACAACTGCAGCCCACGTCACAAAAACTAGATGATACTGAAAATAAGAAGTCGAGTCCCATGGTTGCTATCGTTGCCGGTGTAGTTGCCGTCTTCATTACGCTCTCTGTTCTCATATTCTTGGTTTCGAGGCGACGATGGAAATTAAAGGACTCTGGGTCTGGCTCCAGGCAAACAATGAACCCGGGGCAATTTATGCCTACTAATTTGTGTCGTTACTTTCCGCTGGAAGAGATCACAGCCGCCACCAAAAACTTCAATGATACATTCATTATTGGAGCTGGAGGATTTGGCAACGTATACAAAGGATGCACTGATGGTGGAGCCACTCCAGTTGCCATAAAACGACTGAAACCAGGGTCATCGCAGGGTGCCCACGAGTTTAAGACCGAAATTGAGATGCTCTCCCAACTCCGTCATCGCCATTTGGTTTCATTAATTGGATATTGTGTTGATAAGGGCGAGATGATCTTAGTGTATGATTACATGGCACACGGAACCCTCCGTGACCATCTCTACCACACCGACAACCCGCCTCTTCCCTGGGAGAAGCGACTTCAGATTTGCGTAGGCACCGCTCGAGGGTTGCAATACCTTCATAATGGTGCAAAGGGCACCATCATTCACCGAGATGTGAAGAGCACAAACATATTATTGGATGACAAATGGGTGGCCAAAGTTTCAGATTTTGGGCTGTCTAAGGGCACGACTAACATGTCCGAAACCCACATCAGCACAATGGTGAAAGGCAGTTTTGGGTATCTGGACCCGGAATACTACCGCCGACAACGTCTGACTGAGAAGTCAGACGTGTACTCACTTGGTGTAGTGTTGTTTGAGGTATTGTGTGCAAGGCCAGCAGTGATTCAGACAGAAGAGCTGAAGCAAGTGAGCCTGGCTGAGTGGGCCAAGATCTGTCATCAGAATGGGGACCTTGATCAAATCATTGATCCAAGTTTGAGAGGTAAGATTTCAGCTGACTGCCTGAATAAGTTCACTGAGGTAGCTATCAGTTGCATGCATGATGATGGGACCGAACGACCATCCATGAATGATGTTGTAAAGGAGCTTGAGCTTGCATTGCTGCTTCAGCAGAGTGCTGAAGGGAATACCAAATGCACTGATAGGAATGTTGAAACTGAAACAAGCAGTAGTGACCACAGTTGTGCCACCAACGATTCCGTCAAATGTATATCTGCGACAATCTTCTCTGAGATTAACAACCCAAGTGGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2 Jacalin

Protein Analysis

821

Amino Acids

91.05

Weight (kDa)

6.51

Isoelectric Point (pI)

42.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 37 - 394 2e-24 Malectin-like domain
Pkinase PF00069 500 - 764 2e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 501 - 766 1.5e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000226)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G58037 AT1G58037 AT1G60787 AT1G60787 AT1G60787 AT1G60787 AT1G65180 AT1G69150 AT2G04500 AT2G04680 AT2G19650 AT2G19660 AT2G19660 AT2G21810 AT2G21830 AT2G21830 AT2G21840 AT2G21850 AT2G43220 AT3G06990 AT3G07000 AT3G25850 AT3G27473 AT3G27473 AT3G27473 AT3G27473 AT3G27480 AT3G27480 AT3G27490 AT3G27490 AT3G27500 AT3G27500 AT3G27500 AT3G27510 AT3G27510 AT3G28650 AT3G43890 AT3G45530 AT3G45840 AT3G46800 AT3G46810 AT3G48400 AT4G01350 AT4G01740 AT4G01760 AT4G01910 AT4G01920 AT4G01925 AT4G01930 AT4G02180 AT4G02190 AT4G09690 AT4G11390 AT4G11390 AT4G11540 AT4G11550 AT4G13992 AT4G14980 AT4G16015 AT4G16015 AT5G22355 AT5G26190 AT5G29624 AT5G37620 AT5G39471 AT5G40320 AT5G42840 AT5G42840 AT5G43030 AT5G43040 AT5G44770 AT5G45730 AT5G54020 AT5G54020 AT5G54030 AT5G54040 AT5G54050 AT5G59920 AT5G59930 AT5G59930 AT5G59940
fragaria_vesca FvH4_4g12791 FvH4_6g40980 FvH4_6g41040 FvH4_6g47450 FvH4_7g18770
malus_domestica MD00G1084300.v1.1 MD12G1059900.v1.1 MD14G1057000.v1.1 MD14G1057200.v1.1 MD14G1060000.v1.1 MD14G1060100.v1.1
prunus_persica Prupe.7G074300_v2.0.a1 Prupe.7G074400_v2.0.a1 Prupe.7G074600_v2.0.a1 Prupe.7G074600_v2.0.a1 Prupe.7G078300_v2.0.a1 Prupe.7G078400_v2.0.a1 Prupe.7G078800_v2.0.a1 Prupe.7G081900_v2.0.a1 Prupe.7G092300_v2.0.a1
pyrus_communis pycom14g04870
rosa_chinensis RchiOBHm_Chr1g0362581 RchiOBHm_Chr3g0480191
rosa_laevigata RLG00000023512 RLG00000023513 RLG00000027637
rosa_multiflora Rmu_co8180116.1_g000001 Rmu_co8226133.1_g000001 Rmu_sc0005725.1_g000009 Rmu_sc0006604.1_g000012 Rmu_sc0015624.1_g000001 Rmu_sc0020879.1_g000005 Rmu_sc0020879.1_g000008
rosa_roxburghii Rroxscaffold_4G00293940 Rroxscaffold_6G00401730
rosa_rugosa Rorug01G0299800.1 Rorug03G0180900 Rorug04G0106000 Rorug04G0106100
rosa_samantha Rh1AG308400 Rh1BG271700 Rh1BG271800 Rh1BG272000 Rh1BG272100 Rh1CG289300 Rh1DG302100 Rh1DG302200 Rh1DG302400 Rh1DG302500 Rh3BG264400 Rh3CG262400 Rh3CG262500 Rh3DG258100 Rh3DG258200 Rh3DG258300 Rh3DG273800 Rh4BG170600 Rh7AG404100 Rh7AG404700 Rh7CG422900
rosa_wichuraiana Rw0G000030 Rw1G027380 Rw1G027390 Rw3G020880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1942, 2029
AccB1I GGYRCC 3 cut(s) 1598, 1799, 1841
AccBSI CCGCTC 2 cut(s) 1126, 1805
AccI GTMKAC 1 cut(s) 1527
AccII CGCG 1 cut(s) 220
AccIII TCCGGA 1 cut(s) 334
AclWI GGATC 2 cut(s) 908, 2171
AcoI YGGCCR 4 cut(s) 124, 692, 990, 1899
AcsI RAATTY 4 cut(s) 176, 311, 467, 1201
AcuI CTGAAG 5 cut(s) 576, 1769, 2129, 2300, 2349
AcyI GRCGYC 1 cut(s) 609
AdeI CACNNNGTG 1 cut(s) 2051
AfaI GTAC 4 cut(s) 533, 662, 716, 2042
AfiI CCNNNNNNNGG 5 cut(s) 258, 724, 898, 1082, 1809
AflIII ACRYGT 2 cut(s) 1941, 2037
AgeI ACCGGT 3 cut(s) 65, 230, 572
AgsI TTSAA 7 cut(s) 331, 418, 980, 1162, 1180, 1486, 2363
AhdI GACNNNNNGTC 2 cut(s) 671, 1384
AjiI CACGTC 2 cut(s) 1245, 2038
AjnI CCWGG 6 cut(s) 301, 930, 1394, 1582, 1769, 2121
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
AleI CACNNNNGTG 2 cut(s) 1967, 2391
Alw21I GWGCWC 2 cut(s) 801, 1871
Alw26I GTCTC 3 cut(s) 479, 490, 931
AlwI GGATC 2 cut(s) 908, 2171
Ama87I CYCGRG 4 cut(s) 500, 1192, 1411, 1806
Aor13HI TCCGGA 1 cut(s) 334
AoxI GGCC 8 cut(s) 124, 304, 682, 692, 990, 1899, 2081, 2133
ApeKI GCWGC 2 cut(s) 1237, 2311
ApoI RAATTY 4 cut(s) 176, 311, 467, 1201
ArsI GACNNNNNNTTYG 2 cut(s) 1773, 1805
AseI ATTAAT 1 cut(s) 1664
AsiGI ACCGGT 3 cut(s) 65, 230, 572
Asp700I GAANNNNTTC 1 cut(s) 2219
AspS9I GGNCC 6 cut(s) 683, 1211, 1993, 2133, 2158, 2259
AsuC2I CCSGG 4 cut(s) 89, 1412, 1413, 1997
AsuII TTCGAA 1 cut(s) 958
AvaI CYCGRG 4 cut(s) 500, 1192, 1411, 1806
AvaII GGWCC 4 cut(s) 1211, 1993, 2158, 2259
BaeGI GKGCMC 3 cut(s) 1603, 1844, 1934
BalI TGGCCA 2 cut(s) 992, 1901
BanI GGYRCC 3 cut(s) 1598, 1799, 1841
BanII GRGCYC 1 cut(s) 801
BauI CACGAG 1 cut(s) 1604
BbsI GAAGAC 1 cut(s) 1312
Bbv12I GWGCWC 2 cut(s) 801, 1871
BbvI GCAGC 2 cut(s) 1249, 2298
BceAI ACGGC 2 cut(s) 365, 1301
BcgI CGANNNNNNTGC 2 cut(s) 204, 238
BciT130I CCWGG 6 cut(s) 303, 932, 1396, 1584, 1771, 2123
BciVI GTATCC 1 cut(s) 331
BclI TGATCA 1 cut(s) 2164
BcnI CCSGG 4 cut(s) 89, 1412, 1413, 1997
BcoDI GTCTC 3 cut(s) 479, 490, 931
BfaI CTAG 1 cut(s) 1256
BfmI CTRYAG 1 cut(s) 1235
BfuI GTATCC 1 cut(s) 331
BglII AGATCT 1 cut(s) 2139
BisI GCNGC 4 cut(s) 394, 1238, 1470, 2312
BlsI GCNGC 4 cut(s) 395, 1239, 1471, 2313
Bme18I GGWCC 4 cut(s) 1211, 1993, 2158, 2259
BmeRI GACNNNNNGTC 2 cut(s) 671, 1384
BmeT110I CYCGRG 4 cut(s) 500, 1192, 1411, 1806
BmgBI CACGTC 2 cut(s) 1245, 2038
BmgT120I GGNCC 6 cut(s) 683, 1211, 1993, 2133, 2158, 2259
BmsI GCATC 4 cut(s) 206, 517, 1526, 1617
BpiI GAAGAC 1 cut(s) 1312
BplI GAGNNNNNCTC 2 cut(s) 1616, 1648
BpmI CTGGAG 5 cut(s) 285, 671, 1378, 1530, 1542
Bpu14I TTCGAA 1 cut(s) 958
BpuEI CTTGAG 1 cut(s) 2318
BpuMI CCSGG 4 cut(s) 89, 1412, 1413, 1997
BsaHI GRCGYC 1 cut(s) 609
BsaJI CCNNGG 7 cut(s) 218, 1287, 1411, 1412, 1583, 1769, 1770
BsaWI WCCGGW 6 cut(s) 65, 230, 334, 572, 717, 1078
BsaXI ACNNNNNCTCC 2 cut(s) 644, 674
Bsc4I CCNNNNNNNGG 5 cut(s) 258, 724, 898, 1082, 1809
Bse118I RCCGGY 7 cut(s) 65, 230, 389, 572, 707, 881, 1304
Bse1I ACTGG 3 cut(s) 839, 1040, 1559
Bse3DI GCAATG 2 cut(s) 1156, 2306
BseAI TCCGGA 1 cut(s) 334
BseBI CCWGG 6 cut(s) 303, 932, 1396, 1584, 1771, 2123
BseDI CCNNGG 7 cut(s) 218, 1287, 1411, 1412, 1583, 1769, 1770
BseGI GGATG 8 cut(s) 45, 84, 197, 315, 1011, 1541, 1891, 2270
BseLI CCNNNNNNNGG 5 cut(s) 258, 724, 898, 1082, 1809
BseMI GCAATG 2 cut(s) 1156, 2306
BseMII CTCAG 6 cut(s) 784, 1198, 2016, 2118, 2217, 2430
BseNI ACTGG 3 cut(s) 839, 1040, 1559
BseRI GAGGAG 1 cut(s) 1041
BseSI GKGCMC 3 cut(s) 1603, 1844, 1934
BseXI GCAGC 2 cut(s) 1249, 2298
Bsh1236I CGCG 1 cut(s) 220
Bsh1285I CGRYCG 4 cut(s) 93, 224, 633, 1083
BshFI GGCC 8 cut(s) 126, 306, 684, 694, 992, 1901, 2083, 2135
BshNI GGYRCC 3 cut(s) 1598, 1799, 1841
BshTI ACCGGT 3 cut(s) 65, 230, 572
BsiEI CGRYCG 4 cut(s) 93, 224, 633, 1083
BsiHKAI GWGCWC 2 cut(s) 801, 1871
BsiHKCI CYCGRG 4 cut(s) 500, 1192, 1411, 1806
BsiWI CGTACG 1 cut(s) 531
BslFI GGGAC 6 cut(s) 227, 436, 884, 1269, 2171, 2272
BslI CCNNNNNNNGG 5 cut(s) 258, 724, 898, 1082, 1809
BsmAI GTCTC 3 cut(s) 479, 490, 931
BsmFI GGGAC 6 cut(s) 227, 436, 884, 1269, 2171, 2272
BsnI GGCC 8 cut(s) 126, 306, 684, 694, 992, 1901, 2083, 2135
BsoBI CYCGRG 4 cut(s) 500, 1192, 1411, 1806
Bsp119I TTCGAA 1 cut(s) 958
Bsp1286I GDGCHC 5 cut(s) 801, 1603, 1844, 1871, 1934
Bsp13I TCCGGA 1 cut(s) 334
Bsp143I GATC 8 cut(s) 504, 630, 913, 1461, 1695, 2139, 2164, 2176
Bsp19I CCATGG 1 cut(s) 1287
BspANI GGCC 8 cut(s) 126, 306, 684, 694, 992, 1901, 2083, 2135
BspCNI CTCAG 6 cut(s) 783, 1197, 2017, 2119, 2218, 2431
BspEI TCCGGA 1 cut(s) 334
BspFNI CGCG 1 cut(s) 220
BspMAI CTGCAG 1 cut(s) 1239
BspPI GGATC 2 cut(s) 908, 2171
BspQI GCTCTTC 2 cut(s) 1859, 2097
BspT104I TTCGAA 1 cut(s) 958
BspT107I GGYRCC 3 cut(s) 1598, 1799, 1841
BsrBI CCGCTC 2 cut(s) 1126, 1805
BsrDI GCAATG 2 cut(s) 1156, 2306
BsrFI RCCGGY 7 cut(s) 65, 230, 389, 572, 707, 881, 1304
BsrI ACTGG 3 cut(s) 839, 1040, 1559
BssAI RCCGGY 7 cut(s) 65, 230, 389, 572, 707, 881, 1304
BssECI CCNNGG 7 cut(s) 218, 1287, 1411, 1412, 1583, 1769, 1770
BssMI GATC 8 cut(s) 504, 630, 913, 1461, 1695, 2139, 2164, 2176
BssNAI GTATAC 1 cut(s) 1528
BssNI GRCGYC 1 cut(s) 609
BssSI CACGAG 1 cut(s) 1604
BssT1I CCWWGG 1 cut(s) 1287
Bst1107I GTATAC 1 cut(s) 1528
Bst2BI CACGAG 1 cut(s) 1604
Bst2UI CCWGG 6 cut(s) 303, 932, 1396, 1584, 1771, 2123
Bst4CI ACNGT 1 cut(s) 2390
Bst6I CTCTTC 7 cut(s) 62, 380, 408, 1452, 1770, 1859, 2097
BstACI GRCGYC 1 cut(s) 609
BstAPI GCANNNNNTGC 1 cut(s) 1423
BstBI TTCGAA 1 cut(s) 958
BstC8I GCNNGC 7 cut(s) 323, 391, 619, 776, 2085, 2246, 2304
BstDEI CTNAG 9 cut(s) 770, 910, 1184, 1699, 1926, 2025, 2127, 2226, 2439
BstDSI CCRYGG 2 cut(s) 218, 1287
BstF5I GGATG 8 cut(s) 45, 84, 197, 315, 1011, 1541, 1891, 2270
BstFNI CGCG 1 cut(s) 220
BstKTI GATC 8 cut(s) 507, 633, 916, 1464, 1698, 2142, 2167, 2179
BstMAI GTCTC 3 cut(s) 479, 490, 931
BstMBI GATC 8 cut(s) 504, 630, 913, 1461, 1695, 2139, 2164, 2176
BstMCI CGRYCG 4 cut(s) 93, 224, 633, 1083
BstMWI GCNNNNNNNGC 8 cut(s) 123, 386, 1301, 1423, 2084, 2132, 2308, 2317
BstNI CCWGG 6 cut(s) 303, 932, 1396, 1584, 1771, 2123
BstNSI RCATGY 4 cut(s) 715, 1069, 1945, 2248
BstSFI CTRYAG 1 cut(s) 1235
BstSLI GKGCMC 3 cut(s) 1603, 1844, 1934
BstUI CGCG 1 cut(s) 220
BstV1I GCAGC 2 cut(s) 1249, 2298
BstV2I GAAGAC 1 cut(s) 1312
BstX2I RGATCY 2 cut(s) 913, 2139
BstXI CCANNNNNNTGG 1 cut(s) 950
BstYI RGATCY 2 cut(s) 913, 2139
BstZ17I GTATAC 1 cut(s) 1528
BsuI GTATCC 1 cut(s) 331
BsuRI GGCC 8 cut(s) 126, 306, 684, 694, 992, 1901, 2083, 2135
BtgI CCRYGG 2 cut(s) 218, 1287
BtgZI GCGATG 3 cut(s) 985, 1575, 1631
BtrI CACGTC 2 cut(s) 1245, 2038
BtsCI GGATG 8 cut(s) 45, 84, 197, 315, 1011, 1541, 1891, 2270
BtsI GCAGTG 1 cut(s) 2094
BtsIMutI CAGTG 5 cut(s) 1192, 1539, 2094, 2223, 2346
Cac8I GCNNGC 7 cut(s) 323, 391, 619, 776, 2085, 2246, 2304
Cfr10I RCCGGY 7 cut(s) 65, 230, 389, 572, 707, 881, 1304
Cfr13I GGNCC 6 cut(s) 683, 1211, 1993, 2133, 2158, 2259
Cfr42I CCGCGG 1 cut(s) 221
Cfr9I CCCGGG 1 cut(s) 1411
CseI GACGC 2 cut(s) 446, 617
CsiI ACCWGGT 1 cut(s) 930
Csp6I GTAC 4 cut(s) 532, 661, 715, 2041
CspAI ACCGGT 3 cut(s) 65, 230, 572
CspCI CAANNNNNGTGG 3 cut(s) 1543, 1578, 2437
CviQI GTAC 4 cut(s) 532, 661, 715, 2041
DdeI CTNAG 9 cut(s) 770, 910, 1184, 1699, 1926, 2025, 2127, 2226, 2439
DpnI GATC 8 cut(s) 506, 632, 915, 1463, 1697, 2141, 2166, 2178
DpnII GATC 8 cut(s) 504, 630, 913, 1461, 1695, 2139, 2164, 2176
DraIII CACNNNGTG 1 cut(s) 2051
DrdI GACNNNNNNGTC 2 cut(s) 1942, 2029
DriI GACNNNNNGTC 2 cut(s) 671, 1384
DseDI GACNNNNNNGTC 2 cut(s) 1942, 2029
EaeI YGGCCR 4 cut(s) 124, 692, 990, 1899
Eam1104I CTCTTC 7 cut(s) 62, 380, 408, 1452, 1770, 1859, 2097
Eam1105I GACNNNNNGTC 2 cut(s) 671, 1384
EarI CTCTTC 7 cut(s) 62, 380, 408, 1452, 1770, 1859, 2097
EciI GGCGGA 1 cut(s) 693
Ecl136II GAGCTC 1 cut(s) 799
Eco130I CCWWGG 1 cut(s) 1287
Eco24I GRGCYC 1 cut(s) 801
Eco32I GATATC 1 cut(s) 166
Eco47I GGWCC 4 cut(s) 1211, 1993, 2158, 2259
Eco53kI GAGCTC 1 cut(s) 799
Eco57I CTGAAG 5 cut(s) 576, 1769, 2129, 2300, 2349
Eco88I CYCGRG 4 cut(s) 500, 1192, 1411, 1806
EcoICRI GAGCTC 1 cut(s) 799
EcoO109I RGGNCCY 2 cut(s) 683, 2158
EcoRI GAATTC 1 cut(s) 467
EcoRII CCWGG 6 cut(s) 301, 930, 1394, 1582, 1769, 2121
EcoRV GATATC 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 1287
EcoT22I ATGCAT 1 cut(s) 2250
EcoT38I GRGCYC 1 cut(s) 801
ErhI CCWWGG 1 cut(s) 1287
FaqI GGGAC 6 cut(s) 227, 436, 884, 1269, 2171, 2272
FauI CCCGC 3 cut(s) 357, 462, 1767
FbaI TGATCA 1 cut(s) 2164
FblI GTMKAC 1 cut(s) 1527
Fnu4HI GCNGC 4 cut(s) 394, 1238, 1470, 2312
FokI GGATG 8 cut(s) 32, 71, 184, 302, 998, 1548, 1898, 2257
FriOI GRGCYC 1 cut(s) 801
Fsp4HI GCNGC 4 cut(s) 394, 1238, 1470, 2312
FspBI CTAG 1 cut(s) 1256
GluI GCNGC 4 cut(s) 394, 1238, 1470, 2312
GsuI CTGGAG 5 cut(s) 285, 671, 1378, 1530, 1542
HaeIII GGCC 8 cut(s) 126, 306, 684, 694, 992, 1901, 2083, 2135
HgaI GACGC 2 cut(s) 446, 617
Hin1I GRCGYC 1 cut(s) 609
HincII GTYRAC 1 cut(s) 1102
HindII GTYRAC 1 cut(s) 1102
Hpy166II GTNNAC 9 cut(s) 478, 607, 661, 844, 1045, 1102, 1528, 2041, 2223
Hpy188III TCNNGA 7 cut(s) 335, 484, 502, 650, 818, 1129, 1991
Hpy8I GTNNAC 9 cut(s) 478, 607, 661, 844, 1045, 1102, 1528, 2041, 2223
Hpy99I CGWCG 5 cut(s) 440, 677, 740, 812, 1365
HpyAV CCTTC 4 cut(s) 436, 520, 1832, 2324
HpyCH4III ACNGT 1 cut(s) 2390
HpyCH4IV ACGT 7 cut(s) 672, 873, 1056, 1244, 1524, 2017, 2037
HpyF10VI GCNNNNNNNGC 8 cut(s) 123, 386, 1301, 1423, 2084, 2132, 2308, 2317
HpyF3I CTNAG 9 cut(s) 770, 910, 1184, 1699, 1926, 2025, 2127, 2226, 2439
HpySE526I ACGT 7 cut(s) 672, 873, 1056, 1244, 1524, 2017, 2037
Hsp92I GRCGYC 1 cut(s) 609
Kpn2I TCCGGA 1 cut(s) 334
KroI GCCGGC 1 cut(s) 389
KroNI GCCGGC 1 cut(s) 391
Ksp22I TGATCA 1 cut(s) 2164
KspI CCGCGG 1 cut(s) 221
Kzo9I GATC 8 cut(s) 504, 630, 913, 1461, 1695, 2139, 2164, 2176
LguI GCTCTTC 2 cut(s) 1859, 2097
LmnI GCTCC 7 cut(s) 275, 835, 1131, 1397, 1505, 1550, 2293
Lsp1109I GCAGC 2 cut(s) 1249, 2298
LweI GCATC 4 cut(s) 206, 517, 1526, 1617
MabI ACCWGGT 1 cut(s) 930
MaeI CTAG 1 cut(s) 1256
MaeII ACGT 7 cut(s) 672, 873, 1056, 1244, 1524, 2017, 2037
MaeIII GTNAC 9 cut(s) 61, 191, 382, 874, 1187, 1245, 1442, 1733, 2381
MalI GATC 8 cut(s) 506, 632, 915, 1463, 1697, 2141, 2166, 2178
MbiI CCGCTC 2 cut(s) 1126, 1805
MboI GATC 8 cut(s) 504, 630, 913, 1461, 1695, 2139, 2164, 2176
MfeI CAATTG 1 cut(s) 186
MflI RGATCY 2 cut(s) 913, 2139
MhlI GDGCHC 5 cut(s) 801, 1603, 1844, 1871, 1934
MlsI TGGCCA 2 cut(s) 992, 1901
MluNI TGGCCA 2 cut(s) 992, 1901
MlyI GAGTC 9 cut(s) 164, 249, 458, 492, 660, 933, 1184, 1291, 1373
MmeI TCCRAC 4 cut(s) 297, 382, 998, 1063
Mox20I TGGCCA 2 cut(s) 992, 1901
Mph1103I ATGCAT 1 cut(s) 2250
MreI CGCCGGCG 1 cut(s) 389
MroI TCCGGA 1 cut(s) 334
MroNI GCCGGC 1 cut(s) 389
MroXI GAANNNNTTC 1 cut(s) 2219
MscI TGGCCA 2 cut(s) 992, 1901
MseI TTAA 4 cut(s) 1373, 1611, 1664, 2445
MslI CAYNNNNRTG 9 cut(s) 710, 948, 1070, 1830, 1857, 1967, 2151, 2253, 2391
Msp20I TGGCCA 2 cut(s) 992, 1901
MspA1I CMGCKG 4 cut(s) 220, 457, 1453, 2204
MunI CAATTG 1 cut(s) 186
MvaI CCWGG 6 cut(s) 303, 932, 1396, 1584, 1771, 2123
MvnI CGCG 1 cut(s) 220
MwoI GCNNNNNNNGC 8 cut(s) 123, 386, 1301, 1423, 2084, 2132, 2308, 2317
NaeI GCCGGC 1 cut(s) 391
NciI CCSGG 4 cut(s) 89, 1412, 1413, 1997
NcoI CCATGG 1 cut(s) 1287
NdeII GATC 8 cut(s) 504, 630, 913, 1461, 1695, 2139, 2164, 2176
NgoMIV GCCGGC 1 cut(s) 389
NmeAIII GCCGAG 2 cut(s) 238, 263
NmuCI GTSAC 6 cut(s) 61, 382, 1187, 1245, 1733, 2381
NsiI ATGCAT 1 cut(s) 2250
NspI RCATGY 4 cut(s) 715, 1069, 1945, 2248
NspV TTCGAA 1 cut(s) 958
OliI CACNNNNGTG 2 cut(s) 1967, 2391
PaeI GCATGC 1 cut(s) 2248
PaeR7I CTCGAG 3 cut(s) 500, 1192, 1806
PasI CCCWGGG 1 cut(s) 1770
PciI ACATGT 1 cut(s) 1941
PciSI GCTCTTC 2 cut(s) 1859, 2097
PcsI WCGNNNNNNNCGW 2 cut(s) 444, 792
PdiI GCCGGC 1 cut(s) 391
PdmI GAANNNNTTC 1 cut(s) 2219
PfeI GAWTC 6 cut(s) 203, 549, 955, 1036, 2092, 2405
Pfl23II CGTACG 1 cut(s) 531
PflFI GACNNNGTC 1 cut(s) 2016
PinAI ACCGGT 3 cut(s) 65, 230, 572
PkrI GCNGC 4 cut(s) 395, 1239, 1471, 2313
Ple19I CGATCG 1 cut(s) 633
PleI GAGTC 9 cut(s) 164, 248, 457, 492, 660, 933, 1184, 1290, 1373
PpsI GAGTC 9 cut(s) 164, 248, 457, 492, 660, 933, 1184, 1290, 1373
PpuMI RGGWCCY 1 cut(s) 2158
PscI ACATGT 1 cut(s) 1941
PshBI ATTAAT 1 cut(s) 1664
Psp124BI GAGCTC 1 cut(s) 801
Psp5II RGGWCCY 1 cut(s) 2158
Psp6I CCWGG 6 cut(s) 301, 930, 1394, 1582, 1769, 2121
PspGI CCWGG 6 cut(s) 301, 930, 1394, 1582, 1769, 2121
PspLI CGTACG 1 cut(s) 531
PspPI GGNCC 6 cut(s) 683, 1211, 1993, 2133, 2158, 2259
PspPPI RGGWCCY 1 cut(s) 2158
PspXI VCTCGAGB 2 cut(s) 1192, 1806
PstI CTGCAG 1 cut(s) 1239
PsuI RGATCY 2 cut(s) 913, 2139
PsyI GACNNNGTC 1 cut(s) 2016
PvuI CGATCG 1 cut(s) 633
PvuII CAGCTG 1 cut(s) 2204
RsaI GTAC 4 cut(s) 533, 662, 716, 2042
RsaNI GTAC 4 cut(s) 532, 661, 715, 2041
RseI CAYNNNNRTG 9 cut(s) 710, 948, 1070, 1830, 1857, 1967, 2151, 2253, 2391
SacI GAGCTC 1 cut(s) 801
SacII CCGCGG 1 cut(s) 221
SapI GCTCTTC 2 cut(s) 1859, 2097
SaqAI TTAA 4 cut(s) 1373, 1611, 1664, 2445
SatI GCNGC 4 cut(s) 394, 1238, 1470, 2312
Sau3AI GATC 8 cut(s) 504, 630, 913, 1461, 1695, 2139, 2164, 2176
Sau96I GGNCC 6 cut(s) 683, 1211, 1993, 2133, 2158, 2259
SchI GAGTC 9 cut(s) 164, 249, 458, 492, 660, 933, 1184, 1291, 1373
SduI GDGCHC 5 cut(s) 801, 1603, 1844, 1871, 1934
SexAI ACCWGGT 1 cut(s) 930
SfaNI GCATC 4 cut(s) 206, 517, 1526, 1617
SfcI CTRYAG 1 cut(s) 1235
Sfr274I CTCGAG 3 cut(s) 500, 1192, 1806
Sfr303I CCGCGG 1 cut(s) 221
SfuI TTCGAA 1 cut(s) 958
SgrAI CRCCGGYG 1 cut(s) 389
SgrBI CCGCGG 1 cut(s) 221
SinI GGWCC 4 cut(s) 1211, 1993, 2158, 2259
SlaI CTCGAG 3 cut(s) 500, 1192, 1806
SmaI CCCGGG 1 cut(s) 1413
SmiMI CAYNNNNRTG 9 cut(s) 710, 948, 1070, 1830, 1857, 1967, 2151, 2253, 2391
SmlI CTYRAG 4 cut(s) 500, 1192, 1806, 2297
SmoI CTYRAG 4 cut(s) 500, 1192, 1806, 2297
SphI GCATGC 1 cut(s) 2248
SspMI CTAG 1 cut(s) 1256
SstI GAGCTC 1 cut(s) 801
StyI CCWWGG 1 cut(s) 1287
TaaI ACNGT 1 cut(s) 2390
TaiI ACGT 7 cut(s) 675, 876, 1059, 1247, 1527, 2020, 2040
TaqII GACCGA 3 cut(s) 846, 1631, 2276
TatI WGTACW 1 cut(s) 2040
TauI GCSGC 2 cut(s) 396, 1472
TfiI GAWTC 6 cut(s) 203, 549, 955, 1036, 2092, 2405
Tru1I TTAA 4 cut(s) 1373, 1611, 1664, 2445
Tru9I TTAA 4 cut(s) 1373, 1611, 1664, 2445
TscAI CASTG 5 cut(s) 1192, 1546, 2094, 2230, 2353
TseFI GTSAC 6 cut(s) 61, 382, 1187, 1245, 1733, 2381
TseI GCWGC 2 cut(s) 1237, 2311
Tsp45I GTSAC 6 cut(s) 61, 382, 1187, 1245, 1733, 2381
TspGWI ACGGA 7 cut(s) 508, 561, 1004, 1631, 1721, 1737, 2398
TspMI CCCGGG 1 cut(s) 1411
TspRI CASTG 5 cut(s) 1192, 1546, 2094, 2230, 2353
Tth111I GACNNNGTC 1 cut(s) 2016
VpaK11BI GGWCC 4 cut(s) 1211, 1993, 2158, 2259
VspI ATTAAT 1 cut(s) 1664
XapI RAATTY 4 cut(s) 176, 311, 467, 1201
XceI RCATGY 4 cut(s) 715, 1069, 1945, 2248
XhoI CTCGAG 3 cut(s) 500, 1192, 1806
XmaI CCCGGG 1 cut(s) 1411
XmiI GTMKAC 1 cut(s) 1527
XmnI GAANNNNTTC 1 cut(s) 2219
XspI CTAG 1 cut(s) 1256
Zsp2I ATGCAT 1 cut(s) 2250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.