RchiOBHm_Chr5g0022701

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000048
Physical Location & Seq
Reverse (-)
16830350 .. 16833473
3124 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30262

Sequence Viewer

Length: 3051 bp
ATGGATAGCTTCTGTGCAAACCAATTCAAGCCTTCCGGCTATCGCTTCCTCATCAACAATGTTGCTCATTACTTGCTCTTTCTTTTTCTCGCATCTACATCTCTGCAGACTACTAAACTGTGTTTGGGAGATGGACTTCCCAGTGGTGTGAGATCATCATCATGCATCCAGGAAGAGAGACGAGCGCTTCTCAGCTTCAAAGGAGATGTTACTGATCGTTCCGGCAGGCTTTCTTCTTGGGTGGGTCAGGATTGCTGTCAATGGAAAGGGATTTCATGCAACAACCGTACAGGTCACGTTGTAAAGATGGACCTCCGGAACCTGAATTATGATGAAGGCTTCTACTGGGAGACTTCTTTGGGGGGTAAGATTAATCCTTCTTTGCTTAGCTTTGAGAACTTATATTACCTTGATCTAAGCGGAAACGATTTTCAAGGACGTCGAATTCCTAACTTCTTTGGGAAGCTTCAAAGTTTGAGGTATCTCAATCTCTCATATAATTCATTTGTGGGAGAGATTCCCCCAGCTCTTGGTAACCTCTCAAACTTGAACTATCTTGACCTCAATTCTGTTTATCGCGGTCTTTCTTCCAAAAACTTGAATTGGCTTTCTCATCTCTCTTCCCTAAAGTACCTCAATCTTGGAGGTGTGAACCTTAGCAGCACAGGAGTAAGTTGGCTACATGATGTCAACATGCTTCCTTCACTCTTAGAGTTGCATTTGTCTGTTTGCCAAATTCAAAACCTTCCACACTACTCCCAGCTTCCGTCAGTGAACTTCACATCCCTATTGGTTCTTGATCTATCAGGGAATTCTATTAATTCTTCATTTCCCAGCTGGCTTTTTAATCTTACCAACCTCAAAAAACTTGATGTATCTAGCAACTGGTTCGCGGGTCACTTCCCTGTTGAAATTGCAAACCTCAAGTCTTTGGAAGACCTTCGTTCAAATAATGATTTTCTCCAAGGTCAAATTCCCAAAGTCATTGGTACTTTGTGCAGCCTAATTATATTAGATCTTTCGTCAAACTCGTTCAATGGTAGTTTGGAAAATGTTTTGAATGGCTTCTCAAACTGTACAAGTCATAGATTAGAGTCACTTGATTTATCTTACAATATTATGTTGGAAGGTGAATTGCCTGCCGCCTTGGGAATGCTAGAAAATCTTATGGAACTTCATCCAAGTGGAAACCAATTTTCTGGTTCAATTCCACAATCTATCGATAATTTATCATCCTTGGAAACACTTGACATCTCTGGTAATAATTTGACCGAGTCCATTCCTGAAAGTTTGGGACAACTTTCTCAGCTAGTTCACCTAGATCTATCTCAAAATTTGTGGGTAGGCAATATAACTGAAGCGCATTTCCGATCTCTCACAAAGTTGGAGTTCTTTGCAGTCAATACAGACCTACCTAGGTCCCTCATTTTTAACGTGGCTCATGACTGGGTTCCTCCTTTCCAGCTTGATTCCGTTGACATTGCCAACTGCAGAGCAGGTCCTGCCTTTGGGGTATGGCTTCAGTCTCAAACTGAATTAACGCATCTCAATCTCAGTAATACTGGAATCTCAGATTTCATACCAGAAGAGTGGTTCTTGAAGATTTCTTTCCAACTCACATCGCTGGATTTATCTAACAACCAAATTCGTGGAAGGCTTCCATTTCACATGAACTTCCCATCTTTGGAGTATATAGATTTGAGTCATAATCAATTTGAGGGCCCTCTCCCACATTGGTCTAGTGATGCTGCCGCCTATCTCGATCTTCAAAGCAATTTATTTTCGGGGCCAATTCCCTCAAATTATGGTCAACTGTTGCCTAACTTGCTAAAATTGTATCTATCTGACAATCATCTGAATGGTAGTATACCACCCTCTTTGTGCAACATGTCAGGTCTATATATCTTTATGTTAAGAAGTAATCACTTGTCTGGAGAATTACCTCCAGCATGGAGTCTTTGGCGAGATATATGGGTTGTAGATGTCTCAGACAACAATTTGTCTGGCAATATCCCCAGTTCAATGGGTGTTCCGAGCTCACTGTACGTATTGAATCTGAGGAACAACAATTTTGGGGGTCAGATTCCTTCTTCTCTCTTCCAAAACTGCAGCCACTTGCAGAGTATTGATCTTGGAGGCAATAGATTTACTGGAAGCATACCTTTACAGACAAGATCAATTGTATCATCTGAATTGTATATGCTCAGATTGCGATCCAACTCTTTAAGCGGACATATCCCCAATCGATTGTGCTCTCTTCAATCTCTTCATATCATAGACCTTGGCCACAACAAATTTTCAGGGACCATTCCCAAGTGTTTGTATAATCTGACTGCTTTGGCCTACGGTAATGACACTCGTACGTACTTTTTGTTAAATTATCTTGAGAAGACCAGCTTGACATTGAAGGGACAAGAACTCGTATATAACACCACTCTGAATTTGGTGAATACCATTGATTTCTCATCAAATAACTTAGAAGGTGTAATCCTTGAAGAAATAAGCAGTCTCATTGCATTGGGTACCTTGAACTTGTCAAGAAATCAGTTGAGTGGAAACATTCCTTTGAAGATTGGAAACTTGCGATGGCTGGAAACTCTTGATCTCTCACAAAATCACCTCTCCGGGCAGATTCCTCAAAGTTTCTCTTCATTAACCTCTTTGTCTCACTTGAACTTGTCTTTCAACAACTTGTCAGGCAGAATTCCTTCAGGCAACCAACTTCGAACACTCGATGATTCATCCATTTATGTTGGCAATCCATCCTTGTGTGGGGTTCCTCTTCCAACTGTCTGCCCGGGAGATGGCGCACTCGCACCAGCTGACCCTGACGATGGTGATCATAATGATGATGATACTGATGAGAATGGGAAGTTTGGTATCTATGTTAGCGCTGTCCTCGGCTTCATCATAGGCTTTTGGAGTGTTTGTGGCACATTGGTTATGAAGAAATCATGGAGGTATGCCTATTTTCGATTCTTTGATAACATACAGGAGAGGGCAGCATTAGCAATTGCATTGAGAGTGGCTCGTTGGCAAAGGAAGACTTGA

Protein Analysis

1016

Amino Acids

112.81

Weight (kDa)

5.56

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 57 - 94 1.2e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 123 - 190 8.2e-07 Leucine-rich repeat region
LRR_8 PF13855 133 - 189 1.3e-06 Leucine rich repeat
LRR_14 PF23598 201 - 370 1.2e-08 Leucine-rich repeat region
LRR_14 PF23598 364 - 520 8.2e-13 Leucine-rich repeat region
LRR_8 PF13855 388 - 445 3.9e-07 Leucine rich repeat
LRR_4 PF12799 538 - 572 2e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 824 - 909 9.7e-09 Leucine-rich repeat region
LRR_8 PF13855 860 - 898 3.9e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000048)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34930
fragaria_vesca FvH4_3g12670 FvH4_3g12672 FvH4_3g12740 FvH4_3g12742 FvH4_3g12770 FvH4_3g12770 FvH4_3g12860 FvH4_3g12890 FvH4_3g13431 FvH4_3g13433 FvH4_3g13435 FvH4_3g13470 FvH4_3g13470 FvH4_3g13480 FvH4_3g13562 FvH4_3g13580 FvH4_3g13600 FvH4_3g13600 FvH4_3g13600 FvH4_3g13600 FvH4_3g13600 FvH4_3g13600 FvH4_3g13610 FvH4_3g13620 FvH4_3g13620 FvH4_3g23240 FvH4_3g32910 FvH4_3g35070 FvH4_3g45520 FvH4_5g08430 FvH4_5g08450 FvH4_6g53230 FvH4_6g53300
malus_domestica MD05G1217600.v1.1 MD05G1217700.v1.1 MD05G1224700.v1.1 MD05G1225100.v1.1 MD05G1225300.v1.1 MD05G1225400.v1.1 MD05G1235900.v1.1 MD05G1236100.v1.1 MD05G1236300.v1.1 MD05G1237200.v1.1 MD05G1241600.v1.1 MD05G1245700.v1.1 MD05G1245800.v1.1 MD05G1245900.v1.1 MD05G1246000.v1.1 MD05G1246100.v1.1 MD05G1246300.v1.1 MD05G1246400.v1.1 MD05G1246500.v1.1 MD05G1248100.v1.1 MD05G1248200.v1.1 MD05G1264300.v1.1 MD05G1275900.v1.1 MD07G1051400.v1.1 MD09G1012600.v1.1 MD09G1012700.v1.1 MD09G1012800.v1.1 MD10G1221400.v1.1 MD10G1221500.v1.1 MD10G1221600.v1.1 MD10G1221900.v1.1 MD10G1222200.v1.1 MD10G1222400.v1.1 MD10G1222500.v1.1 MD10G1222900.v1.1 MD10G1223000.v1.1 MD10G1223100.v1.1 MD10G1223500.v1.1 MD10G1228000.v1.1 MD12G1019600.v1.1 MD12G1020000.v1.1 MD12G1020100.v1.1 MD17G1013200.v1.1 MD17G1013300.v1.1 MD17G1013400.v1.1
prunus_persica Prupe.1G060500_v2.0.a1 Prupe.1G100100_v2.0.a1 Prupe.3G124000_v2.0.a1 Prupe.3G304400_v2.0.a1 Prupe.4G116800_v2.0.a1 Prupe.4G116900_v2.0.a1 Prupe.4G117000_v2.0.a1 Prupe.4G121800_v2.0.a1 Prupe.4G121900_v2.0.a1 Prupe.4G122000_v2.0.a1 Prupe.4G122100_v2.0.a1 Prupe.4G122200_v2.0.a1 Prupe.4G122300_v2.0.a1 Prupe.4G122500_v2.0.a1 Prupe.4G122600_v2.0.a1 Prupe.4G122800_v2.0.a1 Prupe.4G122900_v2.0.a1 Prupe.4G123000_v2.0.a1 Prupe.4G123100_v2.0.a1 Prupe.4G123200_v2.0.a1 Prupe.4G131500_v2.0.a1 Prupe.4G131600_v2.0.a1 Prupe.7G032500_v2.0.a1 Prupe.7G032900_v2.0.a1 Prupe.7G033000_v2.0.a1 Prupe.7G033100_v2.0.a1 Prupe.7G033300_v2.0.a1 Prupe.7G033600_v2.0.a1 Prupe.7G034600_v2.0.a1 Prupe.7G040200_v2.0.a1 Prupe.7G059200_v2.0.a1
pyrus_communis pycom05g20580 pycom05g20590 pycom05g20760 pycom05g20800 pycom05g21280 pycom05g21290 pycom05g21820 pycom05g21830 pycom05g22180 pycom05g22190 pycom05g22210 pycom05g22450 pycom05g22460 pycom05g22470 pycom05g22480 pycom05g22490 pycom05g23770 pycom05g23780 pycom05g24050 pycom05g24060 pycom07g03910 pycom10g18700 pycom10g18720 pycom10g18730 pycom10g18740 pycom10g19150 pycom111g01060 pycom12g01660 pycom12g01690 pycom12g01720 pycom12g01740 pycom12g01750 pycom12g01770 pycom12g01810 pycom12g01820 pycom12g01830 pycom12g01840 pycom12g01870 pycom12g01900 pycom17g01060 pycom17g01070
rosa_chinensis RchiOBHm_Chr1g0315041 RchiOBHm_Chr1g0315051 RchiOBHm_Chr1g0315071 RchiOBHm_Chr1g0315161 RchiOBHm_Chr1g0315211 RchiOBHm_Chr1g0315251 RchiOBHm_Chr1g0315321 RchiOBHm_Chr1g0315391 RchiOBHm_Chr1g0315451 RchiOBHm_Chr1g0315481 RchiOBHm_Chr1g0315551 RchiOBHm_Chr1g0315601 RchiOBHm_Chr1g0315621 RchiOBHm_Chr1g0359221 RchiOBHm_Chr2g0142981 RchiOBHm_Chr2g0143001 RchiOBHm_Chr2g0143061 RchiOBHm_Chr2g0143091 RchiOBHm_Chr2g0175321 RchiOBHm_Chr2g0175341 RchiOBHm_Chr2g0175361 RchiOBHm_Chr2g0175371 RchiOBHm_Chr3g0470491 RchiOBHm_Chr3g0480881 RchiOBHm_Chr3g0485511 RchiOBHm_Chr5g0020741 RchiOBHm_Chr5g0020901 RchiOBHm_Chr5g0020931 RchiOBHm_Chr5g0020941 RchiOBHm_Chr5g0020961 RchiOBHm_Chr5g0020971 RchiOBHm_Chr5g0021451 RchiOBHm_Chr5g0021531 RchiOBHm_Chr5g0021831 RchiOBHm_Chr5g0021841 RchiOBHm_Chr5g0021931 RchiOBHm_Chr5g0021941 RchiOBHm_Chr5g0021981 RchiOBHm_Chr5g0021991 RchiOBHm_Chr5g0022011 RchiOBHm_Chr5g0022181 RchiOBHm_Chr5g0022191 RchiOBHm_Chr5g0022211 RchiOBHm_Chr5g0022221 RchiOBHm_Chr5g0022231 RchiOBHm_Chr5g0022291 RchiOBHm_Chr5g0022301 RchiOBHm_Chr5g0022331 RchiOBHm_Chr5g0022361 RchiOBHm_Chr5g0022371 RchiOBHm_Chr5g0022381 RchiOBHm_Chr5g0022401 RchiOBHm_Chr5g0022431 RchiOBHm_Chr5g0022451 RchiOBHm_Chr5g0022541 RchiOBHm_Chr5g0022591 RchiOBHm_Chr5g0022601 RchiOBHm_Chr5g0022701 RchiOBHm_Chr5g0022741 RchiOBHm_Chr5g0022791 RchiOBHm_Chr5g0022811 RchiOBHm_Chr5g0022851 RchiOBHm_Chr5g0029441 RchiOBHm_Chr5g0041631 RchiOBHm_Chr5g0069391 RchiOBHm_Chr6g0273811 RchiOBHm_Chr6g0273831 RchiOBHm_Chr6g0275331 RchiOBHm_Chr7g0219061
rosa_laevigata RLG00000002463 RLG00000020007 RLG00000020008 RLG00000022320 RLG00000022321 RLG00000023446 RLG00000030742 RLG00000030743 RLG00000030747 RLG00000032557 RLG00000032564 RLG00000032571 RLG00000032573 RLG00000032574 RLG00000032612 RLG00000032633 RLG00000032636 RLG00000032638 RLG00000032650 RLG00000032651 RLG00000032652 RLG00000032653 RLG00000032655 RLG00000032673 RLG00000032678 RLG00000032769 RLG00000032857 RLG00000033168 RLG00000033465 RLG00000034086 RLG00000034222 RLG00000035290
rosa_multiflora Rmu_co8143358.1_g000001 Rmu_co8518791.1_g000001 Rmu_sc0000178.1_g000008 Rmu_sc0000178.1_g000014 Rmu_sc0000401.1_g000005 Rmu_sc0000401.1_g000013 Rmu_sc0000401.1_g000017 Rmu_sc0000401.1_g000018 Rmu_sc0000401.1_g000024 Rmu_sc0000401.1_g000025 Rmu_sc0000576.1_g000020 Rmu_sc0001585.1_g000003 Rmu_sc0001585.1_g000005 Rmu_sc0001585.1_g000008 Rmu_sc0001784.1_g000006 Rmu_sc0001784.1_g000011 Rmu_sc0001784.1_g000022 Rmu_sc0001784.1_g000028 Rmu_sc0002052.1_g000004 Rmu_sc0002052.1_g000011 Rmu_sc0002277.1_g000003 Rmu_sc0002277.1_g000006 Rmu_sc0002277.1_g000024 Rmu_sc0002658.1_g000012 Rmu_sc0003512.1_g000004 Rmu_sc0003512.1_g000007 Rmu_sc0004180.1_g000016 Rmu_sc0004180.1_g000029 Rmu_sc0004180.1_g000034 Rmu_sc0005639.1_g000008 Rmu_sc0005639.1_g000023 Rmu_sc0006070.1_g000016 Rmu_sc0006070.1_g000017 Rmu_sc0006966.1_g000021 Rmu_sc0006966.1_g000023 Rmu_sc0007239.1_g000003 Rmu_sc0008460.1_g000010 Rmu_sc0009984.1_g000008 Rmu_sc0010181.1_g000005 Rmu_sc0010181.1_g000010 Rmu_sc0010503.1_g000006 Rmu_sc0011646.1_g000003 Rmu_sc0012984.1_g000011 Rmu_sc0016972.1_g000005 Rmu_sc0016972.1_g000008 Rmu_sc0023070.1_g000003 Rmu_sc0025660.1_g000001 Rmu_ssc0000322.1_g000019
rosa_roxburghii Rroxscaffold_1G00038860 Rroxscaffold_1G00053490 Rroxscaffold_1G00053500 Rroxscaffold_1G00054230 Rroxscaffold_1G00056460 Rroxscaffold_1G00056470 Rroxscaffold_1G00056480 Rroxscaffold_1G00056500 Rroxscaffold_1G00056510 Rroxscaffold_1G00056620 Rroxscaffold_1G00056690 Rroxscaffold_1G00056710 Rroxscaffold_1G00056720 Rroxscaffold_1G00056730 Rroxscaffold_1G00056740 Rroxscaffold_1G00056750 Rroxscaffold_1G00056760 Rroxscaffold_1G00056870 Rroxscaffold_1G00056900 Rroxscaffold_1G00057120 Rroxscaffold_1G00057560 Rroxscaffold_2G00077430 Rroxscaffold_2G00102160 Rroxscaffold_3G00231420 Rroxscaffold_3G00231430 Rroxscaffold_4G00297230 Rroxscaffold_4G00331570 Rroxscaffold_6G00396700 Rroxscaffold_6G00400940 Rroxscaffold_7G00192740
rosa_rugosa Rorug01G0004400 Rorug01G0004500 Rorug02G0377600 Rorug02G0378000 Rorug02G0582800 Rorug02G0583400 Rorug03G0187000 Rorug03G0218800 Rorug04G0081200 Rorug05G0062700 Rorug05G0062700 Rorug05G0063100 Rorug05G0063200 Rorug05G0063200 Rorug05G0063200 Rorug05G0067000 Rorug05G0069700 Rorug05G0069900 Rorug05G0070000 Rorug05G0070100 Rorug05G0070300 Rorug05G0070400 Rorug05G0070600 Rorug05G0070600 Rorug05G0072300 Rorug05G0072400 Rorug05G0072500 Rorug05G0072500 Rorug05G0072600.1 Rorug05G0072700 Rorug05G0072800 Rorug05G0072800 Rorug05G0072900 Rorug05G0073000 Rorug05G0073000 Rorug05G0073400 Rorug05G0073400 Rorug05G0073400 Rorug05G0074800 Rorug05G0075000 Rorug05G0114300 Rorug05G0194200 Rorug06G0097800
rosa_samantha Rh2AG401400 Rh2CG387600 Rh2CG417000 Rh2CG637400 Rh5CG169600 Rh5CG172100 Rh5CG175200 Rh5CG317100 Rh6DG187900
rosa_wichuraiana Rw1G000750 Rw1G000790 Rw1G000820 Rw1G000860 Rw1G000880 Rw1G000910 Rw2G035180 Rw2G035200 Rw2G035240 Rw2G054370 Rw2G054380 Rw5G013570 Rw5G013580 Rw5G013590 Rw5G013910 Rw5G013980 Rw5G014110 Rw5G014120 Rw5G014280 Rw5G014310 Rw5G014400 Rw5G014420 Rw5G014440 Rw5G014460 Rw5G014480 Rw5G014500 Rw5G014520 Rw5G014530 Rw5G042390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 442
Acc36I ACCTGC 1 cut(s) 1487
Acc65I GGTACC 1 cut(s) 2522
AccB1I GGYRCC 1 cut(s) 2522
AccB7I CCANNNNNTGG 1 cut(s) 530
AccI GTMKAC 1 cut(s) 1867
AccII CGCG 2 cut(s) 579, 893
AccIII TCCGGA 1 cut(s) 315
AciI CCGC 6 cut(s) 420, 579, 893, 1143, 1752, 2229
AclWI GGATC 1 cut(s) 2208
AcoI YGGCCR 1 cut(s) 2284
AcsI RAATTY 9 cut(s) 444, 735, 811, 972, 1333, 1644, 2294, 2440, 2703
AcuI CTGAAG 3 cut(s) 1377, 1505, 2694
AcyI GRCGYC 1 cut(s) 439
AfaI GTAC 8 cut(s) 289, 632, 991, 1078, 2045, 2362, 2366, 2524
AfeI AGCGCT 2 cut(s) 186, 2893
AfiI CCNNNNNNNGG 6 cut(s) 530, 1508, 1684, 2772, 2804, 2834
AflIII ACRYGT 1 cut(s) 1887
AjnI CCWGG 1 cut(s) 168
AjuI GAANNNNNNNTTGG 2 cut(s) 957, 989
AloI GAACNNNNNNTCC 2 cut(s) 767, 799
Alw21I GWGCWC 2 cut(s) 2039, 2255
Alw26I GTCTC 6 cut(s) 172, 344, 1530, 1990, 2513, 2670
AlwI GGATC 1 cut(s) 2208
AlwNI CAGNNNCTG 1 cut(s) 1502
Ama87I CYCGRG 1 cut(s) 2797
Aor13HI TCCGGA 1 cut(s) 315
Aor51HI AGCGCT 2 cut(s) 186, 2893
AoxI GGCC 4 cut(s) 1720, 1787, 2284, 2340
ApaI GGGCCC 1 cut(s) 1724
ApeKI GCWGC 5 cut(s) 660, 999, 1748, 2109, 3002
ApoI RAATTY 9 cut(s) 444, 735, 811, 972, 1333, 1644, 2294, 2440, 2703
ArsI GACNNNNNNTTYG 2 cut(s) 911, 943
AseI ATTAAT 2 cut(s) 372, 819
Asp700I GAANNNNTTC 3 cut(s) 939, 1064, 2707
Asp718I GGTACC 1 cut(s) 2522
AspA2I CCTAGG 1 cut(s) 1415
AspLEI GCGC 4 cut(s) 187, 1363, 2810, 2894
AspS9I GGNCC 7 cut(s) 310, 1419, 1499, 1720, 1721, 1787, 2304
AsuC2I CCSGG 3 cut(s) 2626, 2798, 2799
AsuHPI GGTGA 5 cut(s) 1142, 1307, 2458, 2609, 2849
AsuII TTCGAA 1 cut(s) 2725
AvaI CYCGRG 1 cut(s) 2797
AvaII GGWCC 4 cut(s) 310, 1419, 1499, 2304
AvrII CCTAGG 1 cut(s) 1415
BaeGI GKGCMC 1 cut(s) 1724
BalI TGGCCA 1 cut(s) 2286
BanI GGYRCC 1 cut(s) 2522
BanII GRGCYC 2 cut(s) 1724, 2039
BbsI GAAGAC 2 cut(s) 942, 2396
Bbv12I GWGCWC 2 cut(s) 2039, 2255
BbvI GCAGC 5 cut(s) 672, 1011, 1735, 2121, 3014
BccI CCATC 7 cut(s) 125, 301, 1687, 2580, 2770, 2798, 2828
BcgI CGANNNNNNTGC 2 cut(s) 871, 905
BciT130I CCWGG 1 cut(s) 170
BclI TGATCA 1 cut(s) 2839
BcnI CCSGG 3 cut(s) 2626, 2798, 2799
BcoDI GTCTC 6 cut(s) 172, 344, 1530, 1990, 2513, 2670
BfaI CTAG 6 cut(s) 879, 1157, 1310, 1319, 1416, 1740
BfmI CTRYAG 3 cut(s) 104, 1489, 2107
BfoI RGCGCY 2 cut(s) 188, 2895
BfuAI ACCTGC 1 cut(s) 1487
BglII AGATCT 2 cut(s) 1015, 1321
BisI GCNGC 7 cut(s) 661, 1000, 1143, 1749, 1752, 2110, 3003
BlnI CCTAGG 1 cut(s) 1415
BlpI GCTNAGC 1 cut(s) 386
BlsI GCNGC 7 cut(s) 662, 1001, 1144, 1750, 1753, 2111, 3004
Bme1390I CCNGG 4 cut(s) 170, 2626, 2798, 2799
Bme18I GGWCC 4 cut(s) 310, 1419, 1499, 2304
BmeT110I CYCGRG 1 cut(s) 2797
BmgT120I GGNCC 7 cut(s) 310, 1419, 1499, 1720, 1721, 1787, 2304
BmiI GGNNCC 8 cut(s) 320, 1421, 1452, 1722, 1788, 2305, 2524, 2778
BmrFI CCNGG 4 cut(s) 170, 2626, 2798, 2799
BmrI ACTGGG 4 cut(s) 135, 355, 1456, 2010
BmsI GCATC 4 cut(s) 101, 174, 1552, 1735
BmuI ACTGGG 4 cut(s) 135, 355, 1456, 2010
BpiI GAAGAC 2 cut(s) 942, 2396
BplI GAGNNNNNCTC 4 cut(s) 174, 206, 3013, 3045
BpmI CTGGAG 2 cut(s) 1929, 1953
Bpu10I CCTNAGC 1 cut(s) 656
Bpu1102I GCTNAGC 1 cut(s) 386
Bpu14I TTCGAA 1 cut(s) 2725
BpuEI CTTGAG 2 cut(s) 908, 2405
BpuMI CCSGG 3 cut(s) 2626, 2798, 2799
Bsa29I ATCGAT 2 cut(s) 1221, 2245
BsaAI YACGTR 2 cut(s) 2047, 2364
BsaBI GATNNNNATC 3 cut(s) 157, 2212, 2838
BsaHI GRCGYC 1 cut(s) 439
BsaJI CCNNGG 7 cut(s) 964, 1146, 1236, 1415, 2281, 2797, 2899
BsaWI WCCGGW 1 cut(s) 315
BsaXI ACNNNNNCTCC 2 cut(s) 2914, 2944
Bsc4I CCNNNNNNNGG 6 cut(s) 530, 1508, 1684, 2772, 2804, 2834
Bse1I ACTGG 7 cut(s) 141, 350, 890, 1451, 1567, 2016, 2155
Bse3DI GCAATG 2 cut(s) 1479, 2511
Bse8I GATNNNNATC 3 cut(s) 157, 2212, 2838
BseAI TCCGGA 1 cut(s) 315
BseBI CCWGG 1 cut(s) 170
BseCI ATCGAT 2 cut(s) 1221, 2245
BseDI CCNNGG 7 cut(s) 964, 1146, 1236, 1415, 2281, 2797, 2899
BseGI GGATG 6 cut(s) 165, 782, 1177, 1232, 2741, 2762
BseJI GATNNNNATC 3 cut(s) 157, 2212, 2838
BseLI CCNNNNNNNGG 6 cut(s) 530, 1508, 1684, 2772, 2804, 2834
BseMI GCAATG 2 cut(s) 1479, 2511
BseMII CTCAG 7 cut(s) 205, 1319, 1567, 1584, 2001, 2048, 2218
BseNI ACTGG 7 cut(s) 141, 350, 890, 1451, 1567, 2016, 2155
BseSI GKGCMC 1 cut(s) 1724
BseXI GCAGC 5 cut(s) 672, 1011, 1735, 2121, 3014
BseYI CCCAGC 3 cut(s) 523, 759, 833
BsgI GTGCAG 1 cut(s) 1018
Bsh1236I CGCG 2 cut(s) 579, 893
BshFI GGCC 4 cut(s) 1722, 1789, 2286, 2342
BshNI GGYRCC 1 cut(s) 2522
BshVI ATCGAT 2 cut(s) 1221, 2245
BsiHKAI GWGCWC 2 cut(s) 2039, 2255
BsiHKCI CYCGRG 1 cut(s) 2797
BsiSI CCGG 5 cut(s) 36, 222, 316, 2625, 2798
BsiWI CGTACG 1 cut(s) 2360
BslFI GGGAC 4 cut(s) 1308, 1405, 2317, 2424
BslI CCNNNNNNNGG 6 cut(s) 530, 1508, 1684, 2772, 2804, 2834
BsmAI GTCTC 6 cut(s) 172, 344, 1530, 1990, 2513, 2670
BsmBI CGTCTC 1 cut(s) 172
BsmFI GGGAC 4 cut(s) 1308, 1405, 2317, 2424
BsmI GAATGC 1 cut(s) 1158
BsnI GGCC 4 cut(s) 1722, 1789, 2286, 2342
BsoBI CYCGRG 1 cut(s) 2797
Bsp119I TTCGAA 1 cut(s) 2725
Bsp120I GGGCCC 1 cut(s) 1720
Bsp1286I GDGCHC 3 cut(s) 1724, 2039, 2255
Bsp13I TCCGGA 1 cut(s) 315
Bsp1407I TGTACA 1 cut(s) 1076
Bsp1720I GCTNAGC 1 cut(s) 386
BspACI CCGC 6 cut(s) 420, 579, 893, 1143, 1752, 2229
BspANI GGCC 4 cut(s) 1722, 1789, 2286, 2342
BspCNI CTCAG 7 cut(s) 204, 1318, 1566, 1583, 2000, 2049, 2217
BspDI ATCGAT 2 cut(s) 1221, 2245
BspEI TCCGGA 1 cut(s) 315
BspFNI CGCG 2 cut(s) 579, 893
BspHI TCATGA 1 cut(s) 1441
BspLI GGNNCC 8 cut(s) 320, 1421, 1452, 1722, 1788, 2305, 2524, 2778
BspMAI CTGCAG 3 cut(s) 108, 1493, 2111
BspMI ACCTGC 1 cut(s) 1487
BspPI GGATC 1 cut(s) 2208
BspT104I TTCGAA 1 cut(s) 2725
BspT107I GGYRCC 1 cut(s) 2522
BsrDI GCAATG 2 cut(s) 1479, 2511
BsrGI TGTACA 1 cut(s) 1076
BsrI ACTGG 7 cut(s) 141, 350, 890, 1451, 1567, 2016, 2155
BssECI CCNNGG 7 cut(s) 964, 1146, 1236, 1415, 2281, 2797, 2899
BssNAI GTATAC 1 cut(s) 1868
BssNI GRCGYC 1 cut(s) 439
BssT1I CCWWGG 5 cut(s) 964, 1146, 1236, 1415, 2281
Bst1107I GTATAC 1 cut(s) 1868
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 7 cut(s) 120, 287, 1076, 1815, 2043, 2348, 2791
Bst6I CTCTTC 8 cut(s) 168, 625, 1581, 2102, 2262, 2271, 2653, 2787
BstACI GRCGYC 1 cut(s) 439
BstAPI GCANNNNNTGC 1 cut(s) 1502
BstAUI TGTACA 1 cut(s) 1076
BstBAI YACGTR 2 cut(s) 2047, 2364
BstBI TTCGAA 1 cut(s) 2725
BstC8I GCNNGC 3 cut(s) 227, 839, 1140
BstEII GGTNACC 1 cut(s) 533
BstF5I GGATG 6 cut(s) 165, 782, 1177, 1232, 2741, 2762
BstFNI CGCG 2 cut(s) 579, 893
BstH2I RGCGCY 2 cut(s) 188, 2895
BstHHI GCGC 4 cut(s) 187, 1363, 2810, 2894
BstMAI GTCTC 6 cut(s) 172, 344, 1530, 1990, 2513, 2670
BstMWI GCNNNNNNNGC 4 cut(s) 1502, 1825, 2209, 3008
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 2 cut(s) 697, 1891
BstPI GGTNACC 1 cut(s) 533
BstSCI CCNGG 4 cut(s) 168, 2624, 2796, 2797
BstSFI CTRYAG 3 cut(s) 104, 1489, 2107
BstSLI GKGCMC 1 cut(s) 1724
BstSNI TACGTA 2 cut(s) 2047, 2364
BstUI CGCG 2 cut(s) 579, 893
BstV1I GCAGC 5 cut(s) 672, 1011, 1735, 2121, 3014
BstV2I GAAGAC 2 cut(s) 942, 2396
BstX2I RGATCY 2 cut(s) 1015, 1321
BstXI CCANNNNNNTGG 4 cut(s) 1199, 1590, 1649, 2023
BstYI RGATCY 2 cut(s) 1015, 1321
BstZ17I GTATAC 1 cut(s) 1868
Bsu15I ATCGAT 2 cut(s) 1221, 2245
BsuRI GGCC 4 cut(s) 1722, 1789, 2286, 2342
BsuTUI ATCGAT 2 cut(s) 1221, 2245
BtgZI GCGATG 2 cut(s) 1605, 2599
BtsCI GGATG 6 cut(s) 165, 782, 1177, 1232, 2741, 2762
BtsIMutI CAGTG 3 cut(s) 148, 777, 2039
BveI ACCTGC 1 cut(s) 1487
Cac8I GCNNGC 3 cut(s) 227, 839, 1140
CaiI CAGNNNCTG 1 cut(s) 1502
CciI TCATGA 1 cut(s) 1441
CfoI GCGC 4 cut(s) 187, 1363, 2810, 2894
Cfr13I GGNCC 7 cut(s) 310, 1419, 1499, 1720, 1721, 1787, 2304
Cfr9I CCCGGG 1 cut(s) 2797
ClaI ATCGAT 2 cut(s) 1221, 2245
Csp6I GTAC 8 cut(s) 288, 631, 990, 1077, 2044, 2361, 2365, 2523
CspCI CAANNNNNGTGG 4 cut(s) 1319, 1354, 1860, 1895
CviAII CATG 9 cut(s) 162, 276, 683, 694, 1442, 1669, 1888, 1950, 2955
CviQI GTAC 8 cut(s) 288, 631, 990, 1077, 2044, 2361, 2365, 2523
EaeI YGGCCR 1 cut(s) 2284
Eam1104I CTCTTC 8 cut(s) 168, 625, 1581, 2102, 2262, 2271, 2653, 2787
EarI CTCTTC 8 cut(s) 168, 625, 1581, 2102, 2262, 2271, 2653, 2787
Ecl136II GAGCTC 1 cut(s) 2037
Eco105I TACGTA 2 cut(s) 2047, 2364
Eco130I CCWWGG 5 cut(s) 964, 1146, 1236, 1415, 2281
Eco24I GRGCYC 2 cut(s) 1724, 2039
Eco47I GGWCC 4 cut(s) 310, 1419, 1499, 2304
Eco47III AGCGCT 2 cut(s) 186, 2893
Eco53kI GAGCTC 1 cut(s) 2037
Eco57I CTGAAG 3 cut(s) 1377, 1505, 2694
Eco88I CYCGRG 1 cut(s) 2797
Eco91I GGTNACC 1 cut(s) 533
EcoICRI GAGCTC 1 cut(s) 2037
EcoO109I RGGNCCY 4 cut(s) 1419, 1499, 1720, 1721
EcoO65I GGTNACC 1 cut(s) 533
EcoRI GAATTC 3 cut(s) 444, 811, 2703
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 5 cut(s) 964, 1146, 1236, 1415, 2281
EcoT22I ATGCAT 1 cut(s) 167
EcoT38I GRGCYC 2 cut(s) 1724, 2039
ErhI CCWWGG 5 cut(s) 964, 1146, 1236, 1415, 2281
Esp3I CGTCTC 1 cut(s) 172
FaeI CATG 9 cut(s) 165, 279, 686, 697, 1445, 1672, 1891, 1953, 2958
FalI AAGNNNNNCTT 7 cut(s) 2146, 2178, 2381, 2413, 2632, 2664, 3031
FaqI GGGAC 4 cut(s) 1308, 1405, 2317, 2424
FatI CATG 9 cut(s) 161, 275, 682, 693, 1441, 1668, 1887, 1949, 2954
FauI CCCGC 1 cut(s) 886
FbaI TGATCA 1 cut(s) 2839
FblI GTMKAC 1 cut(s) 1867
Fnu4HI GCNGC 7 cut(s) 661, 1000, 1143, 1749, 1752, 2110, 3003
FokI GGATG 6 cut(s) 152, 769, 1164, 1219, 2728, 2749
FriOI GRGCYC 2 cut(s) 1724, 2039
Fsp4HI GCNGC 7 cut(s) 661, 1000, 1143, 1749, 1752, 2110, 3003
FspBI CTAG 6 cut(s) 879, 1157, 1310, 1319, 1416, 1740
GlaI GCGC 4 cut(s) 186, 1362, 2809, 2893
GluI GCNGC 7 cut(s) 661, 1000, 1143, 1749, 1752, 2110, 3003
GsaI CCCAGC 3 cut(s) 527, 763, 837
GsuI CTGGAG 2 cut(s) 1929, 1953
HaeII RGCGCY 2 cut(s) 188, 2895
HaeIII GGCC 4 cut(s) 1722, 1789, 2286, 2342
HapII CCGG 5 cut(s) 36, 222, 316, 2625, 2798
HhaI GCGC 4 cut(s) 187, 1363, 2810, 2894
Hin1I GRCGYC 1 cut(s) 439
Hin1II CATG 9 cut(s) 165, 279, 686, 697, 1445, 1672, 1891, 1953, 2958
Hin6I GCGC 4 cut(s) 185, 1361, 2808, 2892
HinP1I GCGC 4 cut(s) 185, 1361, 2808, 2892
HincII GTYRAC 3 cut(s) 691, 1477, 1811
HindII GTYRAC 3 cut(s) 691, 1477, 1811
HindIII AAGCTT 1 cut(s) 464
HpaII CCGG 5 cut(s) 36, 222, 316, 2625, 2798
HphI GGTGA 5 cut(s) 1142, 1307, 2458, 2609, 2849
Hpy166II GTNNAC 7 cut(s) 652, 691, 775, 1315, 1477, 1811, 1868
Hpy8I GTNNAC 7 cut(s) 652, 691, 775, 1315, 1477, 1811, 1868
Hpy99I CGWCG 1 cut(s) 444
HpyCH4III ACNGT 7 cut(s) 120, 287, 1076, 1815, 2043, 2348, 2791
HpyCH4IV ACGT 5 cut(s) 297, 439, 1434, 2046, 2363
HpyF10VI GCNNNNNNNGC 4 cut(s) 1502, 1825, 2209, 3008
HpySE526I ACGT 5 cut(s) 297, 439, 1434, 2046, 2363
Hsp92I GRCGYC 1 cut(s) 439
Hsp92II CATG 9 cut(s) 165, 279, 686, 697, 1445, 1672, 1891, 1953, 2958
HspAI GCGC 4 cut(s) 185, 1361, 2808, 2892
Kpn2I TCCGGA 1 cut(s) 315
KpnI GGTACC 1 cut(s) 2526
Ksp22I TGATCA 1 cut(s) 2839
Lsp1109I GCAGC 5 cut(s) 672, 1011, 1735, 2121, 3014
LweI GCATC 4 cut(s) 101, 174, 1552, 1735
MaeI CTAG 6 cut(s) 879, 1157, 1310, 1319, 1416, 1740
MaeII ACGT 5 cut(s) 297, 439, 1434, 2046, 2363
MaeIII GTNAC 5 cut(s) 208, 293, 533, 896, 1095
MfeI CAATTG 2 cut(s) 2178, 3012
MflI RGATCY 2 cut(s) 1015, 1321
MhlI GDGCHC 3 cut(s) 1724, 2039, 2255
MlsI TGGCCA 1 cut(s) 2286
MluNI TGGCCA 1 cut(s) 2286
MlyI GAGTC 4 cut(s) 1103, 1283, 1711, 1963
MmeI TCCRAC 5 cut(s) 1104, 1365, 1636, 2241, 2810
Mox20I TGGCCA 1 cut(s) 2286
Mph1103I ATGCAT 1 cut(s) 167
MroI TCCGGA 1 cut(s) 315
MroXI GAANNNNTTC 3 cut(s) 939, 1064, 2707
MscI TGGCCA 1 cut(s) 2286
MseI TTAA 9 cut(s) 372, 819, 846, 1431, 1538, 1913, 2225, 2375, 2654
MslI CAYNNNNRTG 5 cut(s) 160, 1182, 1857, 2767, 2847
Msp20I TGGCCA 1 cut(s) 2286
MspA1I CMGCKG 2 cut(s) 837, 2822
MspI CCGG 5 cut(s) 36, 222, 316, 2625, 2798
MspR9I CCNGG 4 cut(s) 170, 2626, 2798, 2799
MunI CAATTG 2 cut(s) 2178, 3012
Mva1269I GAATGC 1 cut(s) 1158
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 2 cut(s) 579, 893
MwoI GCNNNNNNNGC 4 cut(s) 1502, 1825, 2209, 3008
NciI CCSGG 3 cut(s) 2626, 2798, 2799
NlaIII CATG 9 cut(s) 165, 279, 686, 697, 1445, 1672, 1891, 1953, 2958
NlaIV GGNNCC 8 cut(s) 320, 1421, 1452, 1722, 1788, 2305, 2524, 2778
NmeAIII GCCGAG 1 cut(s) 2880
NmuCI GTSAC 3 cut(s) 293, 896, 1095
NsiI ATGCAT 1 cut(s) 167
NspI RCATGY 2 cut(s) 697, 1891
NspV TTCGAA 1 cut(s) 2725
PagI TCATGA 1 cut(s) 1441
PciI ACATGT 1 cut(s) 1887
PcsI WCGNNNNNNNCGW 1 cut(s) 1028
PctI GAATGC 1 cut(s) 1158
PdmI GAANNNNTTC 3 cut(s) 939, 1064, 2707
PfeI GAWTC 8 cut(s) 517, 1469, 1566, 2053, 2083, 2632, 2738, 2976
Pfl23II CGTACG 1 cut(s) 2360
PflFI GACNNNGTC 1 cut(s) 1273
PflMI CCANNNNNTGG 1 cut(s) 530
PfoI TCCNGGA 1 cut(s) 168
PkrI GCNGC 7 cut(s) 662, 1001, 1144, 1750, 1753, 2111, 3004
PleI GAGTC 4 cut(s) 1102, 1282, 1710, 1962
PpsI GAGTC 4 cut(s) 1102, 1282, 1710, 1962
Ppu21I YACGTR 2 cut(s) 2047, 2364
PpuMI RGGWCCY 2 cut(s) 1419, 1499
PscI ACATGT 1 cut(s) 1887
PshBI ATTAAT 2 cut(s) 372, 819
Psp124BI GAGCTC 1 cut(s) 2039
Psp5II RGGWCCY 2 cut(s) 1419, 1499
Psp6I CCWGG 1 cut(s) 168
PspEI GGTNACC 1 cut(s) 533
PspFI CCCAGC 3 cut(s) 523, 759, 833
PspGI CCWGG 1 cut(s) 168
PspLI CGTACG 1 cut(s) 2360
PspN4I GGNNCC 8 cut(s) 320, 1421, 1452, 1722, 1788, 2305, 2524, 2778
PspOMI GGGCCC 1 cut(s) 1720
PspPI GGNCC 7 cut(s) 310, 1419, 1499, 1720, 1721, 1787, 2304
PspPPI RGGWCCY 2 cut(s) 1419, 1499
PsrI GAACNNNNNNTAC 2 cut(s) 389, 421
PstI CTGCAG 3 cut(s) 108, 1493, 2111
PstNI CAGNNNCTG 1 cut(s) 1502
PsuI RGATCY 2 cut(s) 1015, 1321
PsyI GACNNNGTC 1 cut(s) 1273
PvuII CAGCTG 2 cut(s) 837, 2822
RsaI GTAC 8 cut(s) 289, 632, 991, 1078, 2045, 2362, 2366, 2524
RsaNI GTAC 8 cut(s) 288, 631, 990, 1077, 2044, 2361, 2365, 2523
RseI CAYNNNNRTG 5 cut(s) 160, 1182, 1857, 2767, 2847
SacI GAGCTC 1 cut(s) 2039
SaqAI TTAA 9 cut(s) 372, 819, 846, 1431, 1538, 1913, 2225, 2375, 2654
SatI GCNGC 7 cut(s) 661, 1000, 1143, 1749, 1752, 2110, 3003
Sau96I GGNCC 7 cut(s) 310, 1419, 1499, 1720, 1721, 1787, 2304
SchI GAGTC 4 cut(s) 1103, 1283, 1711, 1963
ScrFI CCNGG 4 cut(s) 170, 2626, 2798, 2799
SduI GDGCHC 3 cut(s) 1724, 2039, 2255
SfaNI GCATC 4 cut(s) 101, 174, 1552, 1735
SfcI CTRYAG 3 cut(s) 104, 1489, 2107
SfuI TTCGAA 1 cut(s) 2725
SinI GGWCC 4 cut(s) 310, 1419, 1499, 2304
SmaI CCCGGG 1 cut(s) 2799
SmiMI CAYNNNNRTG 5 cut(s) 160, 1182, 1857, 2767, 2847
SmlI CTYRAG 2 cut(s) 923, 2384
SmoI CTYRAG 2 cut(s) 923, 2384
SnaBI TACGTA 2 cut(s) 2047, 2364
SsiI CCGC 6 cut(s) 420, 579, 893, 1143, 1752, 2229
SspI AATATT 1 cut(s) 1117
SspMI CTAG 6 cut(s) 879, 1157, 1310, 1319, 1416, 1740
SstI GAGCTC 1 cut(s) 2039
StyD4I CCNGG 4 cut(s) 168, 2624, 2796, 2797
StyI CCWWGG 5 cut(s) 964, 1146, 1236, 1415, 2281
TaaI ACNGT 7 cut(s) 120, 287, 1076, 1815, 2043, 2348, 2791
TaiI ACGT 5 cut(s) 300, 442, 1437, 2049, 2366
TaqI TCGA 7 cut(s) 442, 1221, 1761, 2245, 2725, 2733, 2974
TaqII GACCGA 1 cut(s) 1286
TatI WGTACW 1 cut(s) 1076
TauI GCSGC 2 cut(s) 1145, 1754
TfiI GAWTC 8 cut(s) 517, 1469, 1566, 2053, 2083, 2632, 2738, 2976
Tru1I TTAA 9 cut(s) 372, 819, 846, 1431, 1538, 1913, 2225, 2375, 2654
Tru9I TTAA 9 cut(s) 372, 819, 846, 1431, 1538, 1913, 2225, 2375, 2654
TscAI CASTG 3 cut(s) 148, 777, 2046
TseFI GTSAC 3 cut(s) 293, 896, 1095
TseI GCWGC 5 cut(s) 660, 999, 1748, 2109, 3002
Tsp45I GTSAC 3 cut(s) 293, 896, 1095
TspGWI ACGGA 2 cut(s) 756, 1462
TspMI CCCGGG 1 cut(s) 2797
TspRI CASTG 3 cut(s) 148, 777, 2046
Tth111I GACNNNGTC 1 cut(s) 1273
Van91I CCANNNNNTGG 1 cut(s) 530
VpaK11BI GGWCC 4 cut(s) 310, 1419, 1499, 2304
VspI ATTAAT 2 cut(s) 372, 819
XapI RAATTY 9 cut(s) 444, 735, 811, 972, 1333, 1644, 2294, 2440, 2703
XceI RCATGY 2 cut(s) 697, 1891
XcmI CCANNNNNNNNNTGG 1 cut(s) 2440
XmaI CCCGGG 1 cut(s) 2797
XmaJI CCTAGG 1 cut(s) 1415
XmiI GTMKAC 1 cut(s) 1867
XmnI GAANNNNTTC 3 cut(s) 939, 1064, 2707
XspI CTAG 6 cut(s) 879, 1157, 1310, 1319, 1416, 1740
ZraI GACGTC 1 cut(s) 440
Zsp2I ATGCAT 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.