AT3G13300

Enhancer of mRNA-decapping protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000324
Physical Location & Seq
Forward (+)
4303822 .. 4310275
6454 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G13300.3

Sequence Viewer

Length: 4035 bp
ATGGCGTCTTCTCCTGGTAATACTAACCCTCACAATACTCCTCCGTTTGATCTCGGTATTCTCTTCAAACCTTCATCCAATCCTTATCCGCCACCGGCGGCATCTTATCCTCCGCCTACAGGTCCTTTTCTTCACAACCAGTACGATCAGCAGCACTACGCTCCGCCTGGTATCTCCGCTCAACCATCTCCGGTGACTCAGCAGCAACAGGATGTGTCTTCCTCTTCCGCCGCGACTAACTTGCATCCTCAGAGAACGCTGTCTTATCCCACGCCACCTCTCAATCTACAATCCCCCCGTTCCAATCACAATCCCGGTACGCACATCCTCGCTCTCCTTAACAATACCAACAACGGAGCCCCCGTGGCTAACCAAGAGCCGTCGCATCAGCTCCCAGTAGTCAATCACAACGAGATCGCTCGCTCTTTTCCCGGCGGTTCAGGTCCTATTCGTGTACCGAGCTGTAAATTGCCCAAGGGAAGGCGATTGATTGGTGAACACGCTGTGTACGATGTTGATGTGAGATTACAAGGTGAGATTCAGCCGCAGCTGGAGGTGACTCCGATTACCAAATACGGGTCGGATCCTCAGCTCGTAGTGGGTAGGCAAATCGCCGTGAATAAGGTTTACATTTGCTATGGATTGAAAGGAGGAAACATTAGGGTTCTCAATATCAACACAGCATTGAGGTCTCTGTTCCGTGGCCATTCACAGAGAGTGACGGATATGGCTTTCTTTGCCGAGGATGTTGATATGTTGGCCAGTGTTAGCCTAGATGGAAAAGTTTTTGTGTGGAAAATTTCTGAAGGGTCTGAGGGAGAGGATCAGCCCCAAATAACTGGGAAGATAGTACTTGCTCTTCAGATACTAGGAGAGGAAGACACCAAACATCCACGCGTTTGTTGGCACTGCCACAAACAGGAAATTTTGGTAGTTTCAATTGGTAAACATGTACTGCGTATTGATACTACAAAAGTTGGCAGAGGTGAAGTATTCTCTGCTGAGGCTCCTCTCCAGTGTCCCCTTGATAAGCTGATCGATGGTGTTCAGATTGTTGGTAAGCATGATGGAGAAGTGACAGATTTGTCAATGTGCCAATGGATGACCACGCGTCTGGTTTCTTCTTCAGTTGATGGCACGATTAAGATATGGCAAGATCGTAAGGCACAACCACTTGTAGTTTTGAGGCCTCATGATGGACATCCAGTCAGTTCAGCCACGTTTGTGACATCTCCTGAGAGACCTGATCACATCATACTTATCACGGGGGGTCCTCTAAATCGAGAAATGAAAATCTGGGTCTCTGCTGGGGAAGAAGGGTGGCTTTTACCAGCTGATGCTGAATCATGGAGGTGTACCCAGACTCTTGACTTGAAAAGTTCAACCGAGCCACGAGCTGAAGAGGCATTTTTTAACCAAGTCATAGCATTATCTGAAGCAGGCTTGCTTTTACTTGCAAATGCAAAAAGGAACGCCTTATATGCTGTGCATTTGGACTATGGTTCTTCTCCAGTTGGTACGCGGATGGATTACCTATCAGAGTTTACAGTCACTATGCCCATATTGAGTTTCATTGGGACAAATGATCCTCCAGAAGAACCCATTGTTAAGGTTTATTGTGTTCAGACTCTAGCAATCCAGCAGTATACATTAGACTTGTGCTTATGCTTGCCACCACCTATAGAAAATATGGGTTTGGAGAAGTCAGATTCTAGTGTATCACGAGAGGCAAATCTTGTTGAAGGCATGTCAGAACCATCTGGACTTAAACCTACTGACTTACCTTCAGTTGATTCAGTGCCAAAACCTTCGATTATAGTGAATAGATCAGAAAGTGCTAATAAGTTGAGTTTTCCATCAGCGGAAGCCACATCACAAGCAATTGTTCCACCCAACGGTGAACCTAAAACTTCTGGCCTGCCATCTCAAACCAGTGGTGCAGGTTCTGCGTATGCTACTTTACCCCAACTTCCTCTCAGTCCCAGACTATCAAGTAAACTTTCTGGCTATCACACTCCTGTAGAGGCGATTGAGCCAGTAATACCTCATCATGAGCTTGGTGGCAAAACACCTTCTGCTGATTACTCTGTTGATAGGCAAATGGATGCTGTTGGAGAAAGAAATTTGGACGTGTCATCCGTAGAAGAAATCTCAAGAAGCAAGGACTCAAATGTTACGCCTGATGATGATGTGTCTGGGATGCGAAGCCCATCAGCTTTTTTCAAACACCCCACTCATCTTGTAACTCCTTCAGAGATATTGATGGGCGTTTCTTCCGCTGAAGCCTCCATTACTACTGAAGACAGGAGAGATAGGGATGCAAATATTCAGGACGTGAATAATGATCCAAGAGACACCGAAGTAGAGGTGAAAGAAATAAGTGAAGCAAGGTCAACACAGAATGGTGAAATCAATGATCATGACGAAACTGAGAATTGCACTTCAGAAAATAGAGAAAAAGTCTTCTGCTCACAGGTTTCAAATCTCAGCACTGAGATGGCAAGAGACTGTTATCCTAGTACTGAGGGAACTTTCATTCCAGGGGAATCTAAGGCATATGGACAACCTATAAAGGCTGGAGATGAAAGTGGTGTTGACTCGAGAGGTGGGCCTGCAAAGCTTCTTAAAGGAAAGAAGCAGAAGGCCAAAAATTCACAGGGTCCCGGTTTGTCATCTACATCCTCAAATGTTGCTAATCTGGCTGACTCCTTCAATGAGCAAAGTCAGAGTTTAAGTCATCCCATGACAGATTTACTTCCTCAGTTGTTAGCAATGCAAGAAACGATGAATCAGGTGATGGCTTCGCAGAAGGAGATGCAGAGACAACTATCAAATGCTGCCACTGGCCCTATCGGAAAAGAAAGTAAAAGACTAGAAGTTGCGTTAGGGAGAATGATTGAGAAATCCAGCAAGTCAAATGCTGATGCTCTATGGGCCCGCATCCAGGAGGAGACTGTTAAGAATGAAAAGGCATTACGTGACCATGCCCAGCAAATTGTGAATGCAACGACAAACTTCATGAGCAAGGAGTTAAATGCCATGTTTGAGAAAACGATAAAGAAAGAATTGGCTGCAATTGGTCCAGCCCTAGCACGTTCAGTAGTACCAGTTATTGAAAAAACTGTATCTTCTGCAATCACAGAGTCCTTTCAGAGAGGAATAGGTGACAAAGCAGTTAATCAGCTTGACAAATCTGTTAATATAAAGCTTGAAGCAACCGTAGCTAGGCAAATTCAAGCCCAATTTCAGACCTCTGGCAAGCAAGCCCTCCAGGAAGGTCTTAGGTCAAGTGTGGAGTCCTCAGTCATACCTTCCTTTGAGAAGGCATGCAAGGCCATGTTTGACCAAATAGACTCAGCCTTCCAGAAAGGTATTGCTGAGCATACAAATGCAGCCCAGCAACGGTTTGACTCTGGACACTCCCAGCTTGCTCATACTCTAAAGGAATCAATTACTTCTGCGTCGTCAGTTGCTCAAGCCTTAAGTCGTGAGTTAGCCGAGACCCAAAGGAATCTCTTAGCTCTCGCAGCTGCTGGAGCAAATTCTGGTGGGTCTAATTCCTTGGTTACTCAACTAAGTGGCGGACCTTTGGGTGCTCTTCTTGAAAAGGTTGAAGCACCTATGGACCCAACAACAGAACTATCGAGGTTGATATCTGAACGGAAGTACGAAGAATCTTTCACTTCGGCCCTACAGAGAAGCGATGTCTCTATAGTATCATGGCTTTGCTCACAGGTGGATCTACGTGGATTACTGGCGATGAATCCGCTTCCACTGAGCCAAGGCGTGCTTCTTTCACTGCTGCAGCAGCTAGCCTGTGACATTAGCAAGGACACATCCCGTAAGCTGGCTTGGATGACTGATGTGGTTGCAGCCATAAACCCATCAGACCAGATGATTGCGGTCCACGCTCGGCCAATCTTTGAACAGGTATATCAGATTCTGCACCATCACCGCAACGCACCGGGAAGCGATGTCTCAGCCATCAGACTGATAATGCACGTGATCAACTCCATGCTTATGGGTTGCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000932 GO:0000956 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009892 GO:0009933 GO:0009965 GO:0009987 GO:0010014 GO:0010015 GO:0010016 GO:0010033 GO:0010071 GO:0010072 GO:0010154 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0022414 GO:0022622 GO:0023052 GO:0031086 GO:0031087 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0042221 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046700 GO:0046983 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048532 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071704 GO:0090304 GO:0090305 GO:0090421 GO:0097159 GO:0099402 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
Ge1_WD40

Protein Analysis

1344

Amino Acids

145.72

Weight (kDa)

5.73

Isoelectric Point (pI)

50.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ge1_WD40 PF16529 186 - 498 4.7e-21 WD40 region of Ge1, enhancer of mRNA-decapping protein
WD40_WDHD1_1st PF24817 210 - 322 2.6e-08 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13290 AT3G13300 AT3G13300 AT3G13300
fragaria_vesca FvH4_3g41972 FvH4_3g41980 FvH4_3g41980 FvH4_3g41980 FvH4_3g41980 FvH4_3g42780 FvH4_3g42790 FvH4_3g42820 FvH4_3g42820 FvH4_3g42820 FvH4_3g42820 FvH4_4g01881 FvH4_7g10420
malus_domestica MD00G1013700.v1.1 MD03G1039800.v1.1 MD11G1041300.v1.1 MD11G1094900.v1.1
prunus_persica Prupe.6G032100_v2.0.a1
pyrus_communis pycom03g03070 pycom11g03500 pycom11g08470 pycom12g06730
rosa_chinensis RchiOBHm_Chr1g0328831 RchiOBHm_Chr4g0386471 RchiOBHm_Chr4g0386481 RchiOBHm_Chr4g0386551 RchiOBHm_Chr4g0386701 RchiOBHm_Chr4g0386801 RchiOBHm_Chr4g0386811 RchiOBHm_Chr4g0386821 RchiOBHm_Chr4g0386921 RchiOBHm_Chr4g0386931 RchiOBHm_Chr4g0386941 RchiOBHm_Chr4g0387211 RchiOBHm_Chr4g0389101 RchiOBHm_Chr4g0389111 RchiOBHm_Chr4g0389201 RchiOBHm_Chr4g0389211 RchiOBHm_Chr4g0389321 RchiOBHm_Chr4g0389331 RchiOBHm_Chr4g0389341 RchiOBHm_Chr4g0389411 RchiOBHm_Chr5g0075171
rosa_laevigata RLG00000009985 RLG00000009990 RLG00000009996 RLG00000009998 RLG00000010136 RLG00000010140 RLG00000036537
rosa_multiflora Rmu_co8461959.1_g000001 Rmu_sc0000013.1_g000017 Rmu_sc0000013.1_g000024 Rmu_sc0001015.1_g000001 Rmu_sc0001395.1_g000004 Rmu_sc0001942.1_g000022 Rmu_sc0002498.1_g000018 Rmu_sc0002498.1_g000020 Rmu_sc0002557.1_g000005 Rmu_sc0002604.1_g000010 Rmu_sc0002604.1_g000011 Rmu_sc0004750.1_g000003 Rmu_sc0006571.1_g000001 Rmu_sc0006571.1_g000014 Rmu_sc0006571.1_g000018 Rmu_sc0008050.1_g000016 Rmu_sc0008066.1_g000009 Rmu_sc0008380.1_g000006 Rmu_sc0008380.1_g000010 Rmu_sc0008380.1_g000012 Rmu_sc0010272.1_g000021 Rmu_sc0010693.1_g000010 Rmu_sc0010693.1_g000012 Rmu_sc0010693.1_g000014 Rmu_sc0010693.1_g000015 Rmu_sc0031790.1_g000003 Rmu_sc0040539.1_g000001 Rmu_ssc0000211.1_g000001 Rmu_ssc0000211.1_g000011 Rmu_ssc0000211.1_g000013
rosa_roxburghii Rroxscaffold_1G00006420 Rroxscaffold_1G00006430 Rroxscaffold_5G00334020 Rroxscaffold_5G00334050 Rroxscaffold_5G00335420
rosa_rugosa Rorug03G0307600 Rorug03G0308600 Rorug03G0322400 Rorug05G0437200 Rorug05G0437200 Rorug05G0437200
rosa_samantha Rh1BG075700 Rh4BG010000 Rh4BG010100 Rh4BG010200 Rh4BG020000 Rh4BG020400 Rh4BG172100 Rh4CG015300 Rh4CG016300 Rh5AG494100 Rh5AG494200 Rh5BG515500 Rh5CG539300 Rh5DG528200
rosa_wichuraiana Rw4G000930 Rw4G001980 Rw4G001990 Rw4G011950 Rw5G045850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1084
Acc36I ACCTGC 1 cut(s) 1931
AccBSI CCGCTC 1 cut(s) 179
AccI GTMKAC 1 cut(s) 1646
AccII CGCG 4 cut(s) 233, 897, 1111, 1522
AclWI GGATC 6 cut(s) 578, 591, 831, 1580, 2339, 3747
AcoI YGGCCR 3 cut(s) 703, 759, 3914
AcsI RAATTY 6 cut(s) 798, 924, 2122, 2650, 3231, 3541
AcvI CACGTG 1 cut(s) 4003
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 505
AflII CTTAAG 1 cut(s) 3481
AflIII ACRYGT 4 cut(s) 895, 949, 1109, 2130
AhdI GACNNNNNGTC 2 cut(s) 1110, 1205
AjiI CACGTC 2 cut(s) 2131, 2335
AjnI CCWGG 5 cut(s) 13, 166, 2539, 2943, 3270
AjuI GAANNNNNNNTTGG 2 cut(s) 3774, 3806
AleI CACNNNNGTG 1 cut(s) 1223
AloI GAACNNNNNNTCC 2 cut(s) 2521, 2553
Alw21I GWGCWC 1 cut(s) 3598
AlwI GGATC 6 cut(s) 578, 591, 831, 1580, 2339, 3747
AlwNI CAGNNNCTG 3 cut(s) 1946, 3533, 3943
Ama87I CYCGRG 1 cut(s) 2599
ApaI GGGCCC 1 cut(s) 2938
ApoI RAATTY 6 cut(s) 798, 924, 2122, 2650, 3231, 3541
AsuC2I CCSGG 4 cut(s) 315, 432, 2664, 3966
AsuNHI GCTAGC 1 cut(s) 3811
AvaI CYCGRG 1 cut(s) 2599
AvaII GGWCC 8 cut(s) 122, 443, 1271, 2660, 3080, 3584, 3625, 3904
BaeGI GKGCMC 1 cut(s) 2938
BalI TGGCCA 2 cut(s) 705, 761
BamHI GGATCC 1 cut(s) 583
BanII GRGCYC 2 cut(s) 361, 2938
BarI GAAGNNNNNNTAC 2 cut(s) 843, 875
BauI CACGAG 2 cut(s) 1392, 1722
BbrPI CACGTG 1 cut(s) 4003
BbsI GAAGAC 4 cut(s) 210, 885, 2307, 2455
Bbv12I GWGCWC 1 cut(s) 3598
BbvCI CCTCAGC 2 cut(s) 588, 1002
BceAI ACGGC 2 cut(s) 364, 599
BcgI CGANNNNNNTGC 2 cut(s) 223, 257
BciT130I CCWGG 5 cut(s) 15, 168, 2541, 2945, 3272
BclI TGATCA 3 cut(s) 1246, 2416, 4005
BcnI CCSGG 4 cut(s) 315, 432, 2664, 3966
BfaI CTAG 9 cut(s) 773, 869, 1631, 1713, 2517, 2873, 3089, 3225, 3812
BfmI CTRYAG 6 cut(s) 117, 1680, 2019, 3692, 3711, 3803
BfrI CTTAAG 1 cut(s) 3481
BfuAI ACCTGC 1 cut(s) 1931
BglI GCCNNNNNGGC 1 cut(s) 365
BlpI GCTNAGC 1 cut(s) 3378
BmcAI AGTACT 2 cut(s) 852, 2521
Bme1390I CCNGG 9 cut(s) 15, 168, 315, 432, 2541, 2664, 2945, 3272, 3966
Bme18I GGWCC 8 cut(s) 122, 443, 1271, 2660, 3080, 3584, 3625, 3904
BmeRI GACNNNNNGTC 2 cut(s) 1110, 1205
BmeT110I CYCGRG 1 cut(s) 2599
BmgBI CACGTC 2 cut(s) 2131, 2335
BmiI GGNNCC 8 cut(s) 358, 585, 1008, 1272, 2661, 2662, 2936, 3627
BmrFI CCNGG 9 cut(s) 15, 168, 315, 432, 2541, 2664, 2945, 3272, 3966
BmrI ACTGGG 2 cut(s) 389, 849
BmtI GCTAGC 1 cut(s) 3815
BmuI ACTGGG 2 cut(s) 389, 849
BpiI GAAGAC 4 cut(s) 210, 885, 2307, 2455
BpmI CTGGAG 7 cut(s) 572, 998, 1494, 1575, 2598, 3254, 3555
Bpu10I CCTNAGC 2 cut(s) 588, 1002
Bpu1102I GCTNAGC 1 cut(s) 3378
BpuEI CTTGAG 2 cut(s) 2137, 3459
BpuMI CCSGG 4 cut(s) 315, 432, 2664, 3966
Bsa29I ATCGAT 1 cut(s) 1038
BsaAI YACGTR 3 cut(s) 2978, 3746, 4003
BsaBI GATNNNNATC 1 cut(s) 1200
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 4 cut(s) 696, 1234, 1306, 3494
BsaJI CCNNGG 7 cut(s) 363, 474, 700, 741, 2540, 3561, 3781
BsaWI WCCGGW 1 cut(s) 190
BsaXI ACNNNNNCTCC 2 cut(s) 348, 378
Bse118I RCCGGY 1 cut(s) 94
Bse3DI GCAATG 1 cut(s) 2778
Bse8I GATNNNNATC 1 cut(s) 1200
BseBI CCWGG 5 cut(s) 15, 168, 2541, 2945, 3272
BseCI ATCGAT 1 cut(s) 1038
BseDI CCNNGG 7 cut(s) 363, 474, 700, 741, 2540, 3561, 3781
BseJI GATNNNNATC 1 cut(s) 1200
BseMI GCAATG 1 cut(s) 2778
BseRI GAGGAG 3 cut(s) 30, 999, 2963
BseSI GKGCMC 1 cut(s) 2938
BseYI CCCAGC 4 cut(s) 1307, 2988, 3396, 3423
BsgI GTGCAG 2 cut(s) 1959, 3929
Bsh1236I CGCG 4 cut(s) 233, 897, 1111, 1522
BshVI ATCGAT 1 cut(s) 1038
BsiHKAI GWGCWC 1 cut(s) 3598
BsiHKCI CYCGRG 1 cut(s) 2599
BsiSI CCGG 6 cut(s) 95, 191, 315, 432, 2664, 3965
BslFI GGGAC 4 cut(s) 1005, 1591, 1965, 2646
BsmFI GGGAC 4 cut(s) 1005, 1591, 1965, 2646
BsmI GAATGC 1 cut(s) 3007
Bso31I GGTCTC 4 cut(s) 696, 1234, 1306, 3494
BsoBI CYCGRG 1 cut(s) 2599
Bsp120I GGGCCC 1 cut(s) 2934
Bsp1286I GDGCHC 3 cut(s) 361, 2938, 3598
Bsp1720I GCTNAGC 1 cut(s) 3378
BspDI ATCGAT 1 cut(s) 1038
BspFNI CGCG 4 cut(s) 233, 897, 1111, 1522
BspHI TCATGA 4 cut(s) 1192, 2050, 2419, 3018
BspLI GGNNCC 8 cut(s) 358, 585, 1008, 1272, 2661, 2662, 2936, 3627
BspMAI CTGCAG 1 cut(s) 3807
BspMI ACCTGC 1 cut(s) 1931
BspOI GCTAGC 1 cut(s) 3815
BspPI GGATC 6 cut(s) 578, 591, 831, 1580, 2339, 3747
BspQI GCTCTTC 2 cut(s) 864, 3603
BspTI CTTAAG 1 cut(s) 3481
BspTNI GGTCTC 4 cut(s) 696, 1234, 1306, 3494
BsrBI CCGCTC 1 cut(s) 179
BsrDI GCAATG 1 cut(s) 2778
BsrFI RCCGGY 1 cut(s) 94
BssAI RCCGGY 1 cut(s) 94
BssECI CCNNGG 7 cut(s) 363, 474, 700, 741, 2540, 3561, 3781
BssNAI GTATAC 1 cut(s) 1647
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 2 cut(s) 1392, 1722
BssT1I CCWWGG 3 cut(s) 474, 3561, 3781
Bst1107I GTATAC 1 cut(s) 1647
Bst2BI CACGAG 2 cut(s) 1392, 1722
Bst2UI CCWGG 5 cut(s) 15, 168, 2541, 2945, 3272
Bst4CI ACNGT 7 cut(s) 1549, 1898, 2510, 2956, 3124, 3220, 3405
Bst6I CTCTTC 5 cut(s) 68, 229, 864, 1395, 3603
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 3481
BstAPI GCANNNNNTGC 1 cut(s) 1946
BstBAI YACGTR 3 cut(s) 2978, 3746, 4003
BstDSI CCRYGG 2 cut(s) 363, 700
BstFNI CGCG 4 cut(s) 233, 897, 1111, 1522
BstNI CCWGG 5 cut(s) 15, 168, 2541, 2945, 3272
BstNSI RCATGY 3 cut(s) 953, 1750, 3330
BstSCI CCNGG 9 cut(s) 13, 166, 313, 430, 2539, 2662, 2943, 3270, 3964
BstSFI CTRYAG 6 cut(s) 117, 1680, 2019, 3692, 3711, 3803
BstSLI GKGCMC 1 cut(s) 2938
BstUI CGCG 4 cut(s) 233, 897, 1111, 1522
BstV2I GAAGAC 4 cut(s) 210, 885, 2307, 2455
BstX2I RGATCY 2 cut(s) 583, 3739
BstXI CCANNNNNNTGG 3 cut(s) 839, 1114, 4021
BstYI RGATCY 2 cut(s) 583, 3739
BstZ17I GTATAC 1 cut(s) 1647
Bsu15I ATCGAT 1 cut(s) 1038
BsuTUI ATCGAT 1 cut(s) 1038
BtgI CCRYGG 2 cut(s) 363, 700
BtgZI GCGATG 3 cut(s) 3717, 3773, 3987
BtrI CACGTC 2 cut(s) 2131, 2335
BtsI GCAGTG 2 cut(s) 907, 3797
BtsIMutI CAGTG 9 cut(s) 769, 907, 1022, 1803, 1939, 2490, 2841, 3773, 3797
BveI ACCTGC 1 cut(s) 1931
CaiI CAGNNNCTG 3 cut(s) 1946, 3533, 3943
CciI TCATGA 4 cut(s) 1192, 2050, 2419, 3018
Cfr10I RCCGGY 1 cut(s) 94
ClaI ATCGAT 1 cut(s) 1038
CseI GACGC 2 cut(s) 1100, 3450
CspCI CAANNNNNGTGG 2 cut(s) 2221, 2256
DraIII CACNNNGTG 1 cut(s) 505
DrdI GACNNNNNNGTC 1 cut(s) 1084
DriI GACNNNNNGTC 2 cut(s) 1110, 1205
DseDI GACNNNNNNGTC 1 cut(s) 1084
EaeI YGGCCR 3 cut(s) 703, 759, 3914
Eam1104I CTCTTC 5 cut(s) 68, 229, 864, 1395, 3603
Eam1105I GACNNNNNGTC 2 cut(s) 1110, 1205
EarI CTCTTC 5 cut(s) 68, 229, 864, 1395, 3603
EciI GGCGGA 5 cut(s) 78, 102, 153, 217, 3597
Eco130I CCWWGG 3 cut(s) 474, 3561, 3781
Eco147I AGGCCT 1 cut(s) 1189
Eco24I GRGCYC 2 cut(s) 361, 2938
Eco31I GGTCTC 4 cut(s) 696, 1234, 1306, 3494
Eco32I GATATC 1 cut(s) 3654
Eco47I GGWCC 8 cut(s) 122, 443, 1271, 2660, 3080, 3584, 3625, 3904
Eco72I CACGTG 1 cut(s) 4003
Eco88I CYCGRG 1 cut(s) 2599
EcoO109I RGGNCCY 4 cut(s) 122, 443, 1271, 2660
EcoRII CCWGG 5 cut(s) 13, 166, 2539, 2943, 3270
EcoRV GATATC 1 cut(s) 3654
EcoT14I CCWWGG 3 cut(s) 474, 3561, 3781
EcoT38I GRGCYC 2 cut(s) 361, 2938
ErhI CCWWGG 3 cut(s) 474, 3561, 3781
FalI AAGNNNNNCTT 4 cut(s) 1308, 1340, 3774, 3806
FaqI GGGAC 4 cut(s) 1005, 1591, 1965, 2646
FauI CCCGC 1 cut(s) 2945
FauNDI CATATG 1 cut(s) 2557
FbaI TGATCA 3 cut(s) 1246, 2416, 4005
FblI GTMKAC 1 cut(s) 1646
FriOI GRGCYC 2 cut(s) 361, 2938
FspBI CTAG 9 cut(s) 773, 869, 1631, 1713, 2517, 2873, 3089, 3225, 3812
GsaI CCCAGC 4 cut(s) 1311, 2992, 3400, 3427
GsuI CTGGAG 7 cut(s) 572, 998, 1494, 1575, 2598, 3254, 3555
HapII CCGG 6 cut(s) 95, 191, 315, 432, 2664, 3965
HgaI GACGC 2 cut(s) 1100, 3450
Hin1I GRCGYC 1 cut(s) 5
HincII GTYRAC 2 cut(s) 2394, 2596
HindII GTYRAC 2 cut(s) 2394, 2596
HindIII AAGCTT 2 cut(s) 2618, 3206
HpaII CCGG 6 cut(s) 95, 191, 315, 432, 2664, 3965
Hpy99I CGWCG 2 cut(s) 385, 3466
HpyCH4III ACNGT 7 cut(s) 1549, 1898, 2510, 2956, 3124, 3220, 3405
HpyCH4IV ACGT 7 cut(s) 1220, 2130, 2334, 2977, 3094, 3745, 4002
HpySE526I ACGT 7 cut(s) 1220, 2130, 2334, 2977, 3094, 3745, 4002
Hsp92I GRCGYC 1 cut(s) 5
KflI GGGWCCC 1 cut(s) 2660
Ksp22I TGATCA 3 cut(s) 1246, 2416, 4005
LguI GCTCTTC 2 cut(s) 864, 3603
LmnI GCTCC 5 cut(s) 166, 356, 396, 1012, 3536
MaeI CTAG 9 cut(s) 773, 869, 1631, 1713, 2517, 2873, 3089, 3225, 3812
MaeII ACGT 7 cut(s) 1220, 2130, 2334, 2977, 3094, 3745, 4002
MbiI CCGCTC 1 cut(s) 179
MfeI CAATTG 3 cut(s) 939, 1881, 3075
MflI RGATCY 2 cut(s) 583, 3739
MhlI GDGCHC 3 cut(s) 361, 2938, 3598
MlsI TGGCCA 2 cut(s) 705, 761
MluI ACGCGT 2 cut(s) 895, 1109
MluNI TGGCCA 2 cut(s) 705, 761
MmeI TCCRAC 2 cut(s) 561, 2092
Mox20I TGGCCA 2 cut(s) 705, 761
MscI TGGCCA 2 cut(s) 705, 761
MslI CAYNNNNRTG 5 cut(s) 1223, 1351, 2495, 3387, 4019
Msp20I TGGCCA 2 cut(s) 705, 761
MspA1I CMGCKG 5 cut(s) 550, 1334, 1862, 2279, 3530
MspCI CTTAAG 1 cut(s) 3481
MspI CCGG 6 cut(s) 95, 191, 315, 432, 2664, 3965
MspR9I CCNGG 9 cut(s) 15, 168, 315, 432, 2541, 2664, 2945, 3272, 3966
MunI CAATTG 3 cut(s) 939, 1881, 3075
Mva1269I GAATGC 1 cut(s) 3007
MvaI CCWGG 5 cut(s) 15, 168, 2541, 2945, 3272
MvnI CGCG 4 cut(s) 233, 897, 1111, 1522
NciI CCSGG 4 cut(s) 315, 432, 2664, 3966
NdeI CATATG 1 cut(s) 2557
NheI GCTAGC 1 cut(s) 3811
NlaIV GGNNCC 8 cut(s) 358, 585, 1008, 1272, 2661, 2662, 2936, 3627
NmeAIII GCCGAG 3 cut(s) 766, 3523, 3892
NmuCI GTSAC 9 cut(s) 193, 556, 718, 1075, 1225, 1549, 2978, 3164, 3818
NspI RCATGY 3 cut(s) 953, 1750, 3330
OliI CACNNNNGTG 1 cut(s) 1223
PaeI GCATGC 1 cut(s) 3330
PaeR7I CTCGAG 1 cut(s) 2599
PagI TCATGA 4 cut(s) 1192, 2050, 2419, 3018
PceI AGGCCT 1 cut(s) 1189
PciI ACATGT 1 cut(s) 949
PciSI GCTCTTC 2 cut(s) 864, 3603
PcsI WCGNNNNNNNCGW 3 cut(s) 360, 507, 2136
PctI GAATGC 1 cut(s) 3007
PfoI TCCNGGA 2 cut(s) 2943, 3270
PmaCI CACGTG 1 cut(s) 4003
PmlI CACGTG 1 cut(s) 4003
Ppu21I YACGTR 3 cut(s) 2978, 3746, 4003
PpuMI RGGWCCY 4 cut(s) 122, 443, 1271, 2660
PscI ACATGT 1 cut(s) 949
Psp5II RGGWCCY 4 cut(s) 122, 443, 1271, 2660
Psp6I CCWGG 5 cut(s) 13, 166, 2539, 2943, 3270
PspCI CACGTG 1 cut(s) 4003
PspFI CCCAGC 4 cut(s) 1307, 2988, 3396, 3423
PspGI CCWGG 5 cut(s) 13, 166, 2539, 2943, 3270
PspN4I GGNNCC 8 cut(s) 358, 585, 1008, 1272, 2661, 2662, 2936, 3627
PspOMI GGGCCC 1 cut(s) 2934
PspPPI RGGWCCY 4 cut(s) 122, 443, 1271, 2660
PstI CTGCAG 1 cut(s) 3807
PstNI CAGNNNCTG 3 cut(s) 1946, 3533, 3943
PsuI RGATCY 2 cut(s) 583, 3739
PvuII CAGCTG 3 cut(s) 550, 1334, 3530
RseI CAYNNNNRTG 5 cut(s) 1223, 1351, 2495, 3387, 4019
SapI GCTCTTC 2 cut(s) 864, 3603
ScaI AGTACT 2 cut(s) 852, 2521
ScrFI CCNGG 9 cut(s) 15, 168, 315, 432, 2541, 2664, 2945, 3272, 3966
SduI GDGCHC 3 cut(s) 361, 2938, 3598
SfcI CTRYAG 6 cut(s) 117, 1680, 2019, 3692, 3711, 3803
Sfr274I CTCGAG 1 cut(s) 2599
SgrAI CRCCGGYG 1 cut(s) 94
SinI GGWCC 8 cut(s) 122, 443, 1271, 2660, 3080, 3584, 3625, 3904
SlaI CTCGAG 1 cut(s) 2599
SmiMI CAYNNNNRTG 5 cut(s) 1223, 1351, 2495, 3387, 4019
SmlI CTYRAG 4 cut(s) 2152, 2599, 3474, 3481
SmoI CTYRAG 4 cut(s) 2152, 2599, 3474, 3481
SphI GCATGC 1 cut(s) 3330
SseBI AGGCCT 1 cut(s) 1189
SspI AATATT 1 cut(s) 2326
SspMI CTAG 9 cut(s) 773, 869, 1631, 1713, 2517, 2873, 3089, 3225, 3812
StuI AGGCCT 1 cut(s) 1189
StyD4I CCNGG 9 cut(s) 13, 166, 313, 430, 2539, 2662, 2943, 3270, 3964
StyI CCWWGG 3 cut(s) 474, 3561, 3781
TaaI ACNGT 7 cut(s) 1549, 1898, 2510, 2956, 3124, 3220, 3405
TaiI ACGT 7 cut(s) 1223, 2133, 2337, 2980, 3097, 3748, 4005
TaqI TCGA 5 cut(s) 1038, 1282, 1811, 2600, 3644
TatI WGTACW 3 cut(s) 850, 952, 2519
TauI GCSGC 3 cut(s) 101, 233, 547
TscAI CASTG 9 cut(s) 769, 914, 1022, 1803, 1939, 2497, 2848, 3780, 3804
TseFI GTSAC 9 cut(s) 193, 556, 718, 1075, 1225, 1549, 2978, 3164, 3818
Tsp45I GTSAC 9 cut(s) 193, 556, 718, 1075, 1225, 1549, 2978, 3164, 3818
TspDTI ATGAA 9 cut(s) 63, 1304, 1561, 2524, 2598, 2801, 2979, 3007, 3776
TspGWI ACGGA 6 cut(s) 33, 369, 689, 737, 2128, 3676
TspRI CASTG 9 cut(s) 769, 914, 1022, 1803, 1939, 2497, 2848, 3780, 3804
Vha464I CTTAAG 1 cut(s) 3481
VpaK11BI GGWCC 8 cut(s) 122, 443, 1271, 2660, 3080, 3584, 3625, 3904
XapI RAATTY 6 cut(s) 798, 924, 2122, 2650, 3231, 3541
XceI RCATGY 3 cut(s) 953, 1750, 3330
XcmI CCANNNNNNNNNTGG 1 cut(s) 900
XhoI CTCGAG 1 cut(s) 2599
XmiI GTMKAC 1 cut(s) 1646
XspI CTAG 9 cut(s) 773, 869, 1631, 1713, 2517, 2873, 3089, 3225, 3812
ZrmI AGTACT 2 cut(s) 852, 2521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.