Rorug03G0308600

Enhancer of mRNA-decapping protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000324
Physical Location & Seq
Reverse (-)
32783871 .. 32790345
6475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0308600.1

Sequence Viewer

Length: 1302 bp
ATGATGAACGCGCAGGCTCAGCCGGCAATGGCAGGTCCTCAGGGTTACCCTCATCATCAGCCTACTTCAGTCGAAGAGGTCAGAACCCTCTGGATTGGTGACCTACAGTACTGGGTCGACGAGAACTACCTCACTACTTGCTTTGCTCACACCGGCGAGGTGATTTCTATAAAGATCATACGTAACAAAATCACTGGGCAGCCCGAGGGTTATGGCTTTGTGGAGTTTGTCTCACACGCCGCAGCCGAAAGGATTCTGCATGCTTACAATGGCACTCAAATGCCTGGAACTGAGCAGACTTTTAGGTTGAATTGGGCTTCTTTCGGTATCGGCGAACGGCGTCCTGATGCTGGCCCTGAGCACTCCATTTTCGTAGGGGATTTGGCCCCTGACGTCACCGATTATGTGCTGCAAGAGACTTTCCGGAATACGTATCCGTCCGTTAGAGGTGCCAAGGTTGTCACTGATCCAAACACTGGACGCTCGAAAGGTTATGGCTTTGTCAAGTTTGCTGATGAGGCGGAAAGGAACCGTGCTATGACCGAAATGAATGGTGTTTATTGCTCCACTAGGCCGATGCGGATCAGTGCAGCAACACCAAAGAAAACCACTGGCTTTCAGCAGCAGTATGCTGCAGCAAAAGCTGTATATCCTGTTTCGGCATACACTACACCAGCAGTTCCAGTAGCACCAACAGCAGATTATGATGCAAACAACACTACAATTTTTGTTGGAAACTTGGATCCTAATATTACTGAGGAGGAATTGAAGCAAACTTTTTTGCAATTTGGAGATATTGTCTATGTCAAGATTCCTGCAGGCAAAGGCTGTGGTTTTGTACAGTTTGGGACAAGGGCATCTGCTGAAGAAGCTATACAAAGGATGCAAGGAAAGATGATTGGCCAACAAGTGGTCCGCATTTCTTGGGGTAGGAGCCCAACAGCTAAACAGGATGTACCTGGTAGCTGGGGCCAGCAAGTAGATCCAAATCAATGGAGTGCATATTATGGTTATGGTCAAGGCTATGATCCCTATGCCTATGGAGCTTCTCAAGATCCATCGTTGTATGCATACAGTGGATATGCCGGTTATGGCCAATATCCTCAACAGGTTGAAGGAGTTCAAGATATGGCAGCTATGGCTGGTACTGTTCCATCTTTAGAACAGAGGGATGAACTGGATGATCCCTTGGCTACACCTGATGTTGACAAGTTGAATGCTGCTTACCTCTCTGTACATGGAAGGGCCATTTTGGGCCACCCCTTGTGGCTAAGAACATCAGCGTTTCTGCAGCAAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000932 GO:0000956 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009892 GO:0009933 GO:0009965 GO:0009987 GO:0010014 GO:0010015 GO:0010016 GO:0010033 GO:0010071 GO:0010072 GO:0010154 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0022414 GO:0022622 GO:0023052 GO:0031086 GO:0031087 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0042221 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046700 GO:0046983 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048532 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071704 GO:0090304 GO:0090305 GO:0090421 GO:0097159 GO:0099402 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
Ge1_WD40

Protein Analysis

433

Amino Acids

47.63

Weight (kDa)

5.35

Isoelectric Point (pI)

33.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 30 - 98 1.1e-13 RNA recognition motif
RRM_1 PF00076 123 - 188 2.8e-16 RNA recognition motif
RRM_1 PF00076 242 - 305 7.2e-19 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13290 AT3G13300 AT3G13300 AT3G13300
fragaria_vesca FvH4_3g41972 FvH4_3g41980 FvH4_3g41980 FvH4_3g41980 FvH4_3g41980 FvH4_3g42780 FvH4_3g42790 FvH4_3g42820 FvH4_3g42820 FvH4_3g42820 FvH4_3g42820 FvH4_4g01881 FvH4_7g10420
malus_domestica MD00G1013700.v1.1 MD03G1039800.v1.1 MD11G1041300.v1.1 MD11G1094900.v1.1
prunus_persica Prupe.6G032100_v2.0.a1
pyrus_communis pycom03g03070 pycom11g03500 pycom11g08470 pycom12g06730
rosa_chinensis RchiOBHm_Chr1g0328831 RchiOBHm_Chr4g0386471 RchiOBHm_Chr4g0386481 RchiOBHm_Chr4g0386551 RchiOBHm_Chr4g0386701 RchiOBHm_Chr4g0386801 RchiOBHm_Chr4g0386811 RchiOBHm_Chr4g0386821 RchiOBHm_Chr4g0386921 RchiOBHm_Chr4g0386931 RchiOBHm_Chr4g0386941 RchiOBHm_Chr4g0387211 RchiOBHm_Chr4g0389101 RchiOBHm_Chr4g0389111 RchiOBHm_Chr4g0389201 RchiOBHm_Chr4g0389211 RchiOBHm_Chr4g0389321 RchiOBHm_Chr4g0389331 RchiOBHm_Chr4g0389341 RchiOBHm_Chr4g0389411 RchiOBHm_Chr5g0075171
rosa_laevigata RLG00000009985 RLG00000009990 RLG00000009996 RLG00000009998 RLG00000010136 RLG00000010140 RLG00000036537
rosa_multiflora Rmu_co8461959.1_g000001 Rmu_sc0000013.1_g000017 Rmu_sc0000013.1_g000024 Rmu_sc0001015.1_g000001 Rmu_sc0001395.1_g000004 Rmu_sc0001942.1_g000022 Rmu_sc0002498.1_g000018 Rmu_sc0002498.1_g000020 Rmu_sc0002557.1_g000005 Rmu_sc0002604.1_g000010 Rmu_sc0002604.1_g000011 Rmu_sc0004750.1_g000003 Rmu_sc0006571.1_g000001 Rmu_sc0006571.1_g000014 Rmu_sc0006571.1_g000018 Rmu_sc0008050.1_g000016 Rmu_sc0008066.1_g000009 Rmu_sc0008380.1_g000006 Rmu_sc0008380.1_g000010 Rmu_sc0008380.1_g000012 Rmu_sc0010272.1_g000021 Rmu_sc0010693.1_g000010 Rmu_sc0010693.1_g000012 Rmu_sc0010693.1_g000014 Rmu_sc0010693.1_g000015 Rmu_sc0031790.1_g000003 Rmu_sc0040539.1_g000001 Rmu_ssc0000211.1_g000001 Rmu_ssc0000211.1_g000011 Rmu_ssc0000211.1_g000013
rosa_roxburghii Rroxscaffold_1G00006420 Rroxscaffold_1G00006430 Rroxscaffold_5G00334020 Rroxscaffold_5G00334050 Rroxscaffold_5G00335420
rosa_rugosa Rorug03G0307600 Rorug03G0308600 Rorug03G0322400 Rorug05G0437200 Rorug05G0437200 Rorug05G0437200
rosa_samantha Rh1BG075700 Rh4BG010000 Rh4BG010100 Rh4BG010200 Rh4BG020000 Rh4BG020400 Rh4BG172100 Rh4CG015300 Rh4CG016300 Rh5AG494100 Rh5AG494200 Rh5BG515500 Rh5CG539300 Rh5DG528200
rosa_wichuraiana Rw4G000930 Rw4G001980 Rw4G001990 Rw4G011950 Rw5G045850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 396
Acc36I ACCTGC 1 cut(s) 23
AccB1I GGYRCC 1 cut(s) 449
AccB7I CCANNNNNTGG 2 cut(s) 476, 910
AccI GTMKAC 1 cut(s) 117
AccII CGCG 1 cut(s) 11
AccIII TCCGGA 1 cut(s) 423
AciI CCGC 4 cut(s) 240, 521, 580, 916
AclWI GGATC 8 cut(s) 461, 590, 737, 750, 977, 1022, 1049, 1178
AcoI YGGCCR 2 cut(s) 901, 1093
AcuI CTGAAG 2 cut(s) 51, 885
AcyI GRCGYC 2 cut(s) 340, 393
AfaI GTAC 5 cut(s) 110, 840, 957, 1147, 1236
AfiI CCNNNNNNNGG 3 cut(s) 350, 476, 910
AgsI TTSAA 5 cut(s) 310, 769, 1115, 1124, 1216
AjnI CCWGG 2 cut(s) 283, 958
AluBI AGCT 6 cut(s) 644, 872, 944, 966, 1046, 1136
AluI AGCT 6 cut(s) 644, 872, 944, 966, 1046, 1136
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 2 cut(s) 235, 410
AlwI GGATC 8 cut(s) 461, 590, 737, 750, 977, 1022, 1049, 1178
Ama87I CYCGRG 1 cut(s) 203
Aor13HI TCCGGA 1 cut(s) 423
AoxI GGCC 8 cut(s) 352, 384, 572, 901, 970, 1093, 1245, 1255
Asp700I GAANNNNTTC 2 cut(s) 252, 1119
AspLEI GCGC 1 cut(s) 13
AspS9I GGNCC 7 cut(s) 35, 353, 385, 913, 970, 1245, 1255
AsuHPI GGTGA 3 cut(s) 110, 172, 388
AvaI CYCGRG 1 cut(s) 203
AvaII GGWCC 2 cut(s) 35, 913
AxyI CCTNAGG 1 cut(s) 39
BalI TGGCCA 2 cut(s) 903, 1095
BamHI GGATCC 1 cut(s) 742
BanI GGYRCC 1 cut(s) 449
BanII GRGCYC 1 cut(s) 938
BarI GAAGNNNNNNTAC 2 cut(s) 858, 890
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 9 cut(s) 211, 254, 396, 602, 619, 634, 647, 1145, 1207
BccI CCATC 2 cut(s) 1066, 1162
BceAI ACGGC 1 cut(s) 353
BciT130I CCWGG 2 cut(s) 285, 960
BciVI GTATCC 1 cut(s) 444
BcoDI GTCTC 2 cut(s) 235, 410
BfaI CTAG 1 cut(s) 570
BfmI CTRYAG 4 cut(s) 104, 633, 816, 1289
BfuAI ACCTGC 1 cut(s) 23
BfuI GTATCC 1 cut(s) 444
BlpI GCTNAGC 1 cut(s) 18
BmcAI AGTACT 1 cut(s) 110
Bme1390I CCNGG 2 cut(s) 285, 960
Bme18I GGWCC 2 cut(s) 35, 913
BmeT110I CYCGRG 1 cut(s) 203
BmgT120I GGNCC 7 cut(s) 35, 353, 385, 913, 970, 1245, 1255
BmiI GGNNCC 6 cut(s) 387, 451, 530, 744, 935, 971
BmrFI CCNGG 2 cut(s) 285, 960
BmrI ACTGGG 2 cut(s) 121, 204
BmsI GCATC 5 cut(s) 337, 567, 697, 866, 873
BmuI ACTGGG 2 cut(s) 121, 204
BplI GAGNNNNNCTC 2 cut(s) 215, 247
Bpu10I CCTNAGC 1 cut(s) 357
Bpu1102I GCTNAGC 1 cut(s) 18
BpuEI CTTGAG 1 cut(s) 1035
BsaAI YACGTR 2 cut(s) 182, 432
BsaBI GATNNNNATC 2 cut(s) 581, 987
BsaHI GRCGYC 2 cut(s) 340, 393
BsaJI CCNNGG 3 cut(s) 204, 453, 1188
BsaWI WCCGGW 1 cut(s) 423
BsaXI ACNNNNNCTCC 2 cut(s) 783, 813
Bsc4I CCNNNNNNNGG 3 cut(s) 350, 476, 910
Bse118I RCCGGY 3 cut(s) 22, 152, 1085
Bse1I ACTGG 6 cut(s) 116, 199, 481, 616, 683, 1182
Bse21I CCTNAGG 1 cut(s) 39
Bse3DI GCAATG 1 cut(s) 33
Bse8I GATNNNNATC 2 cut(s) 581, 987
BseAI TCCGGA 1 cut(s) 423
BseBI CCWGG 2 cut(s) 285, 960
BseDI CCNNGG 3 cut(s) 204, 453, 1188
BseGI GGATG 4 cut(s) 888, 958, 1177, 1186
BseJI GATNNNNATC 2 cut(s) 581, 987
BseLI CCNNNNNNNGG 3 cut(s) 350, 476, 910
BseMI GCAATG 1 cut(s) 33
BseMII CTCAG 5 cut(s) 32, 53, 282, 348, 747
BseNI ACTGG 6 cut(s) 116, 199, 481, 616, 683, 1182
BseRI GAGGAG 1 cut(s) 773
BseXI GCAGC 9 cut(s) 211, 254, 396, 602, 619, 634, 647, 1145, 1207
BseYI CCCAGC 1 cut(s) 966
BsgI GTGCAG 1 cut(s) 609
Bsh1236I CGCG 1 cut(s) 11
BshFI GGCC 8 cut(s) 354, 386, 574, 903, 972, 1095, 1247, 1257
BshNI GGYRCC 1 cut(s) 449
BsiHKAI GWGCWC 1 cut(s) 363
BsiHKCI CYCGRG 1 cut(s) 203
BsiSI CCGG 4 cut(s) 23, 153, 424, 1086
BslFI GGGAC 1 cut(s) 862
BslI CCNNNNNNNGG 3 cut(s) 350, 476, 910
BsmAI GTCTC 2 cut(s) 235, 410
BsmFI GGGAC 1 cut(s) 862
BsmI GAATGC 1 cut(s) 1222
BsnI GGCC 8 cut(s) 354, 386, 574, 903, 972, 1095, 1247, 1257
BsoBI CYCGRG 1 cut(s) 203
Bsp1286I GDGCHC 2 cut(s) 363, 938
Bsp13I TCCGGA 1 cut(s) 423
Bsp1407I TGTACA 2 cut(s) 838, 1234
Bsp143I GATC 8 cut(s) 174, 466, 582, 742, 982, 1027, 1054, 1183
Bsp1720I GCTNAGC 1 cut(s) 18
BspACI CCGC 4 cut(s) 240, 521, 580, 916
BspANI GGCC 8 cut(s) 354, 386, 574, 903, 972, 1095, 1247, 1257
BspCNI CTCAG 5 cut(s) 31, 52, 283, 349, 748
BspEI TCCGGA 1 cut(s) 423
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 6 cut(s) 387, 451, 530, 744, 935, 971
BspMAI CTGCAG 3 cut(s) 637, 820, 1293
BspMI ACCTGC 1 cut(s) 23
BspPI GGATC 8 cut(s) 461, 590, 737, 750, 977, 1022, 1049, 1178
BspT107I GGYRCC 1 cut(s) 449
BsrDI GCAATG 1 cut(s) 33
BsrFI RCCGGY 3 cut(s) 22, 152, 1085
BsrGI TGTACA 2 cut(s) 838, 1234
BsrI ACTGG 6 cut(s) 116, 199, 481, 616, 683, 1182
BssAI RCCGGY 3 cut(s) 22, 152, 1085
BssECI CCNNGG 3 cut(s) 204, 453, 1188
BssMI GATC 8 cut(s) 174, 466, 582, 742, 982, 1027, 1054, 1183
BssNI GRCGYC 2 cut(s) 340, 393
BssT1I CCWWGG 2 cut(s) 453, 1188
Bst2UI CCWGG 2 cut(s) 285, 960
Bst4CI ACNGT 5 cut(s) 108, 533, 843, 1076, 1150
Bst6I CTCTTC 1 cut(s) 69
BstACI GRCGYC 2 cut(s) 340, 393
BstAUI TGTACA 2 cut(s) 838, 1234
BstBAI YACGTR 2 cut(s) 182, 432
BstC8I GCNNGC 7 cut(s) 15, 24, 261, 352, 820, 974, 1296
BstDEI CTNAG 6 cut(s) 18, 39, 291, 357, 756, 1271
BstEII GGTNACC 2 cut(s) 44, 98
BstF5I GGATG 4 cut(s) 888, 958, 1177, 1186
BstFNI CGCG 1 cut(s) 11
BstHHI GCGC 1 cut(s) 13
BstKTI GATC 8 cut(s) 177, 469, 585, 745, 985, 1030, 1057, 1186
BstMAI GTCTC 2 cut(s) 235, 410
BstMBI GATC 8 cut(s) 174, 466, 582, 742, 982, 1027, 1054, 1183
BstMWI GCNNNNNNNGC 8 cut(s) 19, 23, 518, 641, 695, 869, 1043, 1139
BstNI CCWGG 2 cut(s) 285, 960
BstNSI RCATGY 1 cut(s) 263
BstPI GGTNACC 2 cut(s) 44, 98
BstSCI CCNGG 2 cut(s) 283, 958
BstSFI CTRYAG 4 cut(s) 104, 633, 816, 1289
BstSNI TACGTA 2 cut(s) 182, 432
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 9 cut(s) 211, 254, 396, 602, 619, 634, 647, 1145, 1207
BstX2I RGATCY 3 cut(s) 742, 982, 1054
BstXI CCANNNNNNTGG 1 cut(s) 993
BstYI RGATCY 3 cut(s) 742, 982, 1054
Bsu36I CCTNAGG 1 cut(s) 39
BsuI GTATCC 1 cut(s) 444
BsuRI GGCC 8 cut(s) 354, 386, 574, 903, 972, 1095, 1247, 1257
BtsCI GGATG 4 cut(s) 888, 958, 1177, 1186
BtsIMutI CAGTG 6 cut(s) 192, 462, 474, 592, 609, 1081
BveI ACCTGC 1 cut(s) 23
Cac8I GCNNGC 7 cut(s) 15, 24, 261, 352, 820, 974, 1296
CfoI GCGC 1 cut(s) 13
Cfr10I RCCGGY 3 cut(s) 22, 152, 1085
Cfr13I GGNCC 7 cut(s) 35, 353, 385, 913, 970, 1245, 1255
CseI GACGC 2 cut(s) 329, 489
CsiI ACCWGGT 1 cut(s) 958
Csp6I GTAC 5 cut(s) 109, 839, 956, 1146, 1235
CspCI CAANNNNNGTGG 2 cut(s) 811, 846
CviAII CATG 2 cut(s) 260, 1238
CviQI GTAC 5 cut(s) 109, 839, 956, 1146, 1235
DdeI CTNAG 6 cut(s) 18, 39, 291, 357, 756, 1271
DpnI GATC 8 cut(s) 176, 468, 584, 744, 984, 1029, 1056, 1185
DpnII GATC 8 cut(s) 174, 466, 582, 742, 982, 1027, 1054, 1183
EaeI YGGCCR 2 cut(s) 901, 1093
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
EciI GGCGGA 1 cut(s) 536
Eco105I TACGTA 2 cut(s) 182, 432
Eco130I CCWWGG 2 cut(s) 453, 1188
Eco24I GRGCYC 1 cut(s) 938
Eco47I GGWCC 2 cut(s) 35, 913
Eco57I CTGAAG 2 cut(s) 51, 885
Eco81I CCTNAGG 1 cut(s) 39
Eco88I CYCGRG 1 cut(s) 203
Eco91I GGTNACC 2 cut(s) 44, 98
EcoO109I RGGNCCY 1 cut(s) 35
EcoO65I GGTNACC 2 cut(s) 44, 98
EcoRII CCWGG 2 cut(s) 283, 958
EcoT14I CCWWGG 2 cut(s) 453, 1188
EcoT22I ATGCAT 1 cut(s) 1072
EcoT38I GRGCYC 1 cut(s) 938
ErhI CCWWGG 2 cut(s) 453, 1188
FaeI CATG 2 cut(s) 263, 1241
FaqI GGGAC 1 cut(s) 862
FatI CATG 2 cut(s) 259, 1237
FblI GTMKAC 1 cut(s) 117
FokI GGATG 4 cut(s) 895, 965, 1184, 1193
FriOI GRGCYC 1 cut(s) 938
FspBI CTAG 1 cut(s) 570
GlaI GCGC 1 cut(s) 12
GsaI CCCAGC 1 cut(s) 970
HaeIII GGCC 8 cut(s) 354, 386, 574, 903, 972, 1095, 1247, 1257
HapII CCGG 4 cut(s) 23, 153, 424, 1086
HgaI GACGC 2 cut(s) 329, 489
HhaI GCGC 1 cut(s) 13
Hin1I GRCGYC 2 cut(s) 340, 393
Hin1II CATG 2 cut(s) 263, 1241
Hin6I GCGC 1 cut(s) 11
HinP1I GCGC 1 cut(s) 11
HincII GTYRAC 2 cut(s) 118, 1207
HindII GTYRAC 2 cut(s) 118, 1207
HinfI GANTC 2 cut(s) 253, 811
HpaII CCGG 4 cut(s) 23, 153, 424, 1086
HphI GGTGA 3 cut(s) 110, 172, 388
Hpy166II GTNNAC 2 cut(s) 118, 1207
Hpy188I TCNGA 1 cut(s) 83
Hpy188III TCNNGA 6 cut(s) 91, 344, 424, 808, 1052, 1124
Hpy8I GTNNAC 2 cut(s) 118, 1207
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 2 cut(s) 1109, 1236
HpyCH4III ACNGT 5 cut(s) 108, 533, 843, 1076, 1150
HpyCH4IV ACGT 3 cut(s) 181, 393, 431
HpyF10VI GCNNNNNNNGC 8 cut(s) 19, 23, 518, 641, 695, 869, 1043, 1139
HpyF3I CTNAG 6 cut(s) 18, 39, 291, 357, 756, 1271
HpySE526I ACGT 3 cut(s) 181, 393, 431
Hsp92I GRCGYC 2 cut(s) 340, 393
Hsp92II CATG 2 cut(s) 263, 1241
HspAI GCGC 1 cut(s) 11
Kpn2I TCCGGA 1 cut(s) 423
KroI GCCGGC 1 cut(s) 22
KroNI GCCGGC 1 cut(s) 24
Kzo9I GATC 8 cut(s) 174, 466, 582, 742, 982, 1027, 1054, 1183
LmnI GCTCC 3 cut(s) 569, 933, 1043
Lsp1109I GCAGC 9 cut(s) 211, 254, 396, 602, 619, 634, 647, 1145, 1207
LweI GCATC 5 cut(s) 337, 567, 697, 866, 873
MabI ACCWGGT 1 cut(s) 958
MaeI CTAG 1 cut(s) 570
MaeII ACGT 3 cut(s) 181, 393, 431
MaeIII GTNAC 5 cut(s) 44, 98, 182, 394, 460
MalI GATC 8 cut(s) 176, 468, 584, 744, 984, 1029, 1056, 1185
MboI GATC 8 cut(s) 174, 466, 582, 742, 982, 1027, 1054, 1183
MboII GAAGA 2 cut(s) 86, 878
MflI RGATCY 3 cut(s) 742, 982, 1054
MhlI GDGCHC 2 cut(s) 363, 938
MlsI TGGCCA 2 cut(s) 903, 1095
MluCI AATT 4 cut(s) 310, 723, 764, 785
MluNI TGGCCA 2 cut(s) 903, 1095
MmeI TCCRAC 1 cut(s) 712
Mox20I TGGCCA 2 cut(s) 903, 1095
Mph1103I ATGCAT 1 cut(s) 1072
MroI TCCGGA 1 cut(s) 423
MroNI GCCGGC 1 cut(s) 22
MroXI GAANNNNTTC 2 cut(s) 252, 1119
MscI TGGCCA 2 cut(s) 903, 1095
MslI CAYNNNNRTG 1 cut(s) 278
Msp20I TGGCCA 2 cut(s) 903, 1095
MspI CCGG 4 cut(s) 23, 153, 424, 1086
MspR9I CCNGG 2 cut(s) 285, 960
Mva1269I GAATGC 1 cut(s) 1222
MvaI CCWGG 2 cut(s) 285, 960
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 8 cut(s) 19, 23, 518, 641, 695, 869, 1043, 1139
NaeI GCCGGC 1 cut(s) 24
NdeII GATC 8 cut(s) 174, 466, 582, 742, 982, 1027, 1054, 1183
NgoMIV GCCGGC 1 cut(s) 22
NlaIII CATG 2 cut(s) 263, 1241
NlaIV GGNNCC 6 cut(s) 387, 451, 530, 744, 935, 971
NmuCI GTSAC 3 cut(s) 98, 394, 460
NsiI ATGCAT 1 cut(s) 1072
NspI RCATGY 1 cut(s) 263
PaeI GCATGC 1 cut(s) 263
PcsI WCGNNNNNNNCGW 2 cut(s) 243, 330
PctI GAATGC 1 cut(s) 1222
PdiI GCCGGC 1 cut(s) 24
PdmI GAANNNNTTC 2 cut(s) 252, 1119
PfeI GAWTC 2 cut(s) 253, 811
PflMI CCANNNNNTGG 2 cut(s) 476, 910
Ppu21I YACGTR 2 cut(s) 182, 432
PpuMI RGGWCCY 1 cut(s) 35
Psp5II RGGWCCY 1 cut(s) 35
Psp6I CCWGG 2 cut(s) 283, 958
PspEI GGTNACC 2 cut(s) 44, 98
PspFI CCCAGC 1 cut(s) 966
PspGI CCWGG 2 cut(s) 283, 958
PspN4I GGNNCC 6 cut(s) 387, 451, 530, 744, 935, 971
PspPI GGNCC 7 cut(s) 35, 353, 385, 913, 970, 1245, 1255
PspPPI RGGWCCY 1 cut(s) 35
PstI CTGCAG 3 cut(s) 637, 820, 1293
PsuI RGATCY 3 cut(s) 742, 982, 1054
RsaI GTAC 5 cut(s) 110, 840, 957, 1147, 1236
RsaNI GTAC 5 cut(s) 109, 839, 956, 1146, 1235
RseI CAYNNNNRTG 1 cut(s) 278
SalI GTCGAC 1 cut(s) 116
Sau3AI GATC 8 cut(s) 174, 466, 582, 742, 982, 1027, 1054, 1183
Sau96I GGNCC 7 cut(s) 35, 353, 385, 913, 970, 1245, 1255
SbfI CCTGCAGG 1 cut(s) 820
ScaI AGTACT 1 cut(s) 110
ScrFI CCNGG 2 cut(s) 285, 960
SdaI CCTGCAGG 1 cut(s) 820
SduI GDGCHC 2 cut(s) 363, 938
SexAI ACCWGGT 1 cut(s) 958
SfaNI GCATC 5 cut(s) 337, 567, 697, 866, 873
SfcI CTRYAG 4 cut(s) 104, 633, 816, 1289
SgrAI CRCCGGYG 1 cut(s) 152
SinI GGWCC 2 cut(s) 35, 913
SmiMI CAYNNNNRTG 1 cut(s) 278
SmlI CTYRAG 1 cut(s) 1050
SmoI CTYRAG 1 cut(s) 1050
SnaBI TACGTA 2 cut(s) 182, 432
SphI GCATGC 1 cut(s) 263
Sse8387I CCTGCAGG 1 cut(s) 820
Sse9I AATT 4 cut(s) 310, 723, 764, 785
SsiI CCGC 4 cut(s) 240, 521, 580, 916
SspI AATATT 1 cut(s) 751
SspMI CTAG 1 cut(s) 570
StyD4I CCNGG 2 cut(s) 283, 958
StyI CCWWGG 2 cut(s) 453, 1188
TaaI ACNGT 5 cut(s) 108, 533, 843, 1076, 1150
TaiI ACGT 3 cut(s) 184, 396, 434
TaqI TCGA 3 cut(s) 72, 117, 485
TaqII GACCGA 1 cut(s) 557
TasI AATT 4 cut(s) 310, 723, 764, 785
TatI WGTACW 3 cut(s) 108, 838, 1234
TauI GCSGC 1 cut(s) 242
TfiI GAWTC 2 cut(s) 253, 811
TscAI CASTG 6 cut(s) 199, 469, 481, 592, 616, 1081
TseFI GTSAC 3 cut(s) 98, 394, 460
Tsp45I GTSAC 3 cut(s) 98, 394, 460
TspDTI ATGAA 3 cut(s) 20, 563, 1188
TspGWI ACGGA 2 cut(s) 426, 430
TspRI CASTG 6 cut(s) 199, 469, 481, 592, 616, 1081
Van91I CCANNNNNTGG 2 cut(s) 476, 910
VpaK11BI GGWCC 2 cut(s) 35, 913
XceI RCATGY 1 cut(s) 263
XmiI GTMKAC 1 cut(s) 117
XmnI GAANNNNTTC 2 cut(s) 252, 1119
XspI CTAG 1 cut(s) 570
ZraI GACGTC 1 cut(s) 394
ZrmI AGTACT 1 cut(s) 110
Zsp2I ATGCAT 1 cut(s) 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.