MD11G1041300.v1.1

Enhancer of mRNA-decapping protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000324
Physical Location & Seq
Forward (+)
3527115 .. 3534959
7845 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1041300.v1.1.491

Sequence Viewer

Length: 4170 bp
ATGGCTGCCACCGGGAATCAAAATCCGCCGCCGCAGCAGCAACCGCCGCCCTTCGACATGCAGAAATTCTTTAAACCCACAAGCCCATCTCCTCTCACCCCCCAAACCTCAACCCCCGCCCATTCAAACCAAAACCCTAACCCTAATTTGAGTTCCTCCTCGTTCCCAATCCCGCCGGCTTCTTTCCCTCCTCCCACGGGGCCCTACTCGTACCCGCCCCAAACGGCACCGTTTCACCTCCATCAGTTTCAATACCACCCCCACCCGCAGCAGCCCCACCCCCAAATGCCTTACTCTCCGCAAGACCACCACTTGCTCCACCAGAGGTCTCTCTCGTTTCCCACCCCGCCCCTCCAACCCCCTTCTAATTACAACATTGCCACTCCCGCTTCCAACCCCAACAGCACCGGCGGTAGCAATTCCAATTCGAGCCCGAATTCTGGTGCTCGAATCATGGCGCTTCTTGGTGCTCCTTCGGGAAATCTAGAGCCTGAGTTGTCGACTCCTACAGGTGTCTCAATGGTGCCCGCTCTCCCTATGGGGATTCCATCCACTGGCCCGACTCGAATGCCCAGCAATAAGCTGCCGAAAGGCCGGCATTTGATTGGCGATAACGTGGTTTATGATGTGGACGTTAGGTTGCAGGGTGAGGTTCAGCCACAGCTCGAAGTTACGCCCATAACCAAGTATGGTTCGGATCCTCAGCTTGTGCTTGGACGCCAAATTGCGGTTAATAAGTCGTATATATGCTATGGATTGAAGCAGGGGAACATTCGGGTGTTGAATATACACACTGCATTGAGATCTTTGTTTCGAGCTCATACGCAGAGGGTCACAGACATGGCTTTCTTTGCTGAGGATGTTCACCTTTTGGCTAGTGTAAGTGTAGAAGGACGACTCTTTGTATGGAAGATTTCTGAAGGTCCAGATGAAGAAGGTACGCCACAAATCACTGGAAAGGTTGTCATTGCTATTCACATAGTTGGAGAGGGGGAAGCTATTCATCCACGAGTTTGTTGGCACTGTCATAAACAAGAGGTTTTGGTAGTTGGGTTTGGAAAGCGTGTACTAAGAATTGATACTACTAAAGTTGTGAAGGGTGAAGCTCCTTCTGCTGATGAGCCTGTCAAATGTCCCGTTGAAAAGCTGATTGATGGAGTCCAGTTTGTCGGTAAACATGATGGAGAAGTGACCGATTTGTCAATGTGCCAATGGATGACCACCCGTCTGGTTTCTGCTTCTATGGATGGAACAATAAAGATCTGGGAAGATCGCAAGGCTCAACCTCTTTTAGTATTGAGACCCTATGACGGCCAACCTGTTTATTCAGCTACATTTGTGACAGCTCCCCACAGGCCAGATCATATTATACTTATCACAGTGGGGCCACTTAATCGGGAAGTGAAGATCTGGTCCTCAGCGAGTGAAGAAGGCTGGCTGCTTCCCAGTGATGCTGAATCATGGAAATGTACCCAGACATTAGAGTTGAAGAGTTCTTCTGAACCCCGAGTCGAGGAGGCATTCTTTAATCAAGTCATAGCATTGTCACAAGCTGGCCTTCTCTTACTTGCAAATGCTAAGAAGAATGCTATATATGCTGTACACTTAGAATTTGGTTCTGATCCAGCATCAACCCGCATGGATTACATTGCAGAATTTACTGTTACCATGCCAATTTTGAGTTTTACAGGGACAAGTATATCTTCTCATGGTGAACAGATTGTTCAGGTTTATTGTGTCCAGACACTGGCCATTCAGCAGTATGCTTTGGAATTATTAAAGTGCTTGCCTCCACCACTGGATAATGTGGGATTAGAAAAGTCAGATTCCAATATCTCACGCGATGCAATTGGGGCTGAAGGATATCTATCTGGAAGTAAACCCACAGAGGCACTGCTAGCGAATTCATCACCTAAACCAGCCGTACAAGAAAGTAGTTCTGAGGGTGCAGCTGCAAATACAGAATCCAGACTTGTTTCATTTGCATCGGCAACTAGTGACTCTGATGCTGTTTTTGTTGCAACACCACCTATTACTTTGAGTCCTAAGTTGTCTGGGAAATTTTCTGGTATAAGAAGTCCAGCAGATATCTCTGAGGCAGGTCGCACACTTAATGACCATGGTGGAGACCAACAAGTCAATGATTACTCAGTTGACAGGCAAATGGACCCTGCTCATTTAAACATGTCGGATGTGCCTGCAGTGGATGATGACTCGAGGAATGATGAACCTAAAGTTGGACAAGATGATTTATCTAGTGTGCTCAATCCTCCTATCATGTTCAAACATCCAACCCATCTAATAACTCCCTCTGAGATTTTAATGGCTGCTTCATCTTCTGAAGCTGCTACTATTGACAGCAAGAGTGAGGGGGAGGGAAATATCCAAGATGTTCTTGTTAACAGTGATGTAGGTAATTCTGAGGTGGAGATGAAAGTAGTGGGTGAAACGAGATCTACTCAAATTGATGAGTTTGGCTCTCAAGGAGAACCACAAAATGCTGTCTCAGAAAATAAAGAAAAATACTTTTGCTCCCAGGCATCAGATCTTGGAATTGAAATGGCCCGAGAATGCTGTGCCCTACCAGCAGAAAATTATGTAACGGATGAAGCTCGGCAAGTTGATGATGCCAGCGTGACAGAACCACCAGGCCAGTCTCATGCTGGAGACGAAGACCAGGACTCTGCAAAAGATGTTTCTGCGTCATCTACCCCCCCAACAGTTTTGCAATCGCAGACATCAAATACAAAAGTGAAAAAACAGAAGTGGAAAAATTCACAAGCATCAGGTTCATCGTTCCCATCAGCAACTGTACTCAATTCAATAGATTCCACCAATGAAAATGGTGTCAGTTCAAGCCTACCCTCTTCAGAAGCTGCACACCCTCAAATTATGGCCATGCAGGATACGGTTAATCAGCTACTGACCATGCAGAAAGAAATGCAAAAGCAGATGACAATGATGGTTGCAGGCCCTGTTACAAAGGAAGGGAGGAGATTGGAAGCTGCTCTTGGGCGAAGTATGGAGAAGACTGTGAAGGCCAATAATGACGCTCTATGGGCTCGTTTTCAAGAAGAGAATGCAAAGAACGAGAAGTTATTGCGAGACCGTAATCAGCAAATAACCAGTTTGATCAATAACTTCATAAACAAGGAATTCCCAGTCATGTTGGAGAAAGTGGTGAAGAAGGAATTATCTGTGATTGGACCAGCTGTTGTTCGTGCTATAACTCCAGCGATTGAGAAGGCCATACCTTTGGCTATTTCTGATTCCTTCCAGAGAGGAGTGGGTGACAAGGCTGTTAATCAATTGGATAAATCTGTTAACTCAAAACTTGAAGCTACTGTTTCCAGGCAAATTCAGGCGCAGTTTCAAACTTCTGGCAAACAAGCTCTTCAGGATGCTCTCAAGTCTAGTATGGAAGCTTCGGTAGTACCTGCCTTTGAGAAGTCATGTAAGGCCATGTTTGAGCAAGTAGATGCTACGTTCCAGAAAGGAATGGTTGAACACACAACTGCGGCCCAGCAGCACTTTGACTCCGCACATTCACCATTGGCACTGGCTCTAAGGGAAGCTATTAATTCTGCATCATCGGTGACTCAAACCTTAAGCGGAGAAGTGGCTGATGGTCAACGTAAGCTCATAGCTCTGGCAGCTGCACGGGGAAACTCAAGTGCAGTAAATCCTATGGTCACACAACTCACTAATGGACCTTTGGGTGGTCTCCACGAGAAGGTTGAGGTGCCTTTGGATCCAAAGAATGAGCTATCGAGGCTGGTATCTGAACGCAAGTACGAGGAGGCTTTCACAGTGGCTCTGCAAAGAAGTGATGTGACCATCGTATCGTGGTTGTGCCATCAGGTTGATCTGCGTGGGATATTGCTGTCGAATCCTGTTCCCCTGAGCCAAGGTGTGCTTCTTTCTCTTTTGCAACAACTGGCGTGTGATATCAGCAATGATACACCCAGAAAAGTTGCATGGATGACGGATGTGGCGGGCGCCATAAACCCGGCTAACCAAATGATTGCGATGCACGTACGGCCTATATTTGAGCAAGTTTACAATATACTGCACCATCAACATGCCTTGCCTACGGTCTCTAGTGCCGAACACACAAGTATCCGACTTCTCATGCATGTCATCAACTCCATGATGATGGCCTGTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000932 GO:0000956 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009892 GO:0009933 GO:0009965 GO:0009987 GO:0010014 GO:0010015 GO:0010016 GO:0010033 GO:0010071 GO:0010072 GO:0010154 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0022414 GO:0022622 GO:0023052 GO:0031086 GO:0031087 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0042221 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046700 GO:0046983 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048532 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071704 GO:0090304 GO:0090305 GO:0090421 GO:0097159 GO:0099402 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
Ge1_WD40

Protein Analysis

1390

Amino Acids

150.59

Weight (kDa)

5.62

Isoelectric Point (pI)

46.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ge1_WD40 PF16529 224 - 537 8.2e-19 WD40 region of Ge1, enhancer of mRNA-decapping protein
WD40_WDHD1_1st PF24817 242 - 359 4.5e-06 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 247 - 474 1.1e-07 WDR5 beta-propeller domain
EDC4_C PF21289 1258 - 1363 8.2e-17 Enhancer of mRNA-decapping protein 4, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13290 AT3G13300 AT3G13300 AT3G13300
fragaria_vesca FvH4_3g41972 FvH4_3g41980 FvH4_3g41980 FvH4_3g41980 FvH4_3g41980 FvH4_3g42780 FvH4_3g42790 FvH4_3g42820 FvH4_3g42820 FvH4_3g42820 FvH4_3g42820 FvH4_4g01881 FvH4_7g10420
malus_domestica MD00G1013700.v1.1 MD03G1039800.v1.1 MD11G1041300.v1.1 MD11G1094900.v1.1
prunus_persica Prupe.6G032100_v2.0.a1
pyrus_communis pycom03g03070 pycom11g03500 pycom11g08470 pycom12g06730
rosa_chinensis RchiOBHm_Chr1g0328831 RchiOBHm_Chr4g0386471 RchiOBHm_Chr4g0386481 RchiOBHm_Chr4g0386551 RchiOBHm_Chr4g0386701 RchiOBHm_Chr4g0386801 RchiOBHm_Chr4g0386811 RchiOBHm_Chr4g0386821 RchiOBHm_Chr4g0386921 RchiOBHm_Chr4g0386931 RchiOBHm_Chr4g0386941 RchiOBHm_Chr4g0387211 RchiOBHm_Chr4g0389101 RchiOBHm_Chr4g0389111 RchiOBHm_Chr4g0389201 RchiOBHm_Chr4g0389211 RchiOBHm_Chr4g0389321 RchiOBHm_Chr4g0389331 RchiOBHm_Chr4g0389341 RchiOBHm_Chr4g0389411 RchiOBHm_Chr5g0075171
rosa_laevigata RLG00000009985 RLG00000009990 RLG00000009996 RLG00000009998 RLG00000010136 RLG00000010140 RLG00000036537
rosa_multiflora Rmu_co8461959.1_g000001 Rmu_sc0000013.1_g000017 Rmu_sc0000013.1_g000024 Rmu_sc0001015.1_g000001 Rmu_sc0001395.1_g000004 Rmu_sc0001942.1_g000022 Rmu_sc0002498.1_g000018 Rmu_sc0002498.1_g000020 Rmu_sc0002557.1_g000005 Rmu_sc0002604.1_g000010 Rmu_sc0002604.1_g000011 Rmu_sc0004750.1_g000003 Rmu_sc0006571.1_g000001 Rmu_sc0006571.1_g000014 Rmu_sc0006571.1_g000018 Rmu_sc0008050.1_g000016 Rmu_sc0008066.1_g000009 Rmu_sc0008380.1_g000006 Rmu_sc0008380.1_g000010 Rmu_sc0008380.1_g000012 Rmu_sc0010272.1_g000021 Rmu_sc0010693.1_g000010 Rmu_sc0010693.1_g000012 Rmu_sc0010693.1_g000014 Rmu_sc0010693.1_g000015 Rmu_sc0031790.1_g000003 Rmu_sc0040539.1_g000001 Rmu_ssc0000211.1_g000001 Rmu_ssc0000211.1_g000011 Rmu_ssc0000211.1_g000013
rosa_roxburghii Rroxscaffold_1G00006420 Rroxscaffold_1G00006430 Rroxscaffold_5G00334020 Rroxscaffold_5G00334050 Rroxscaffold_5G00335420
rosa_rugosa Rorug03G0307600 Rorug03G0308600 Rorug03G0322400 Rorug05G0437200 Rorug05G0437200 Rorug05G0437200
rosa_samantha Rh1BG075700 Rh4BG010000 Rh4BG010100 Rh4BG010200 Rh4BG020000 Rh4BG020400 Rh4BG172100 Rh4CG015300 Rh4CG016300 Rh5AG494100 Rh5AG494200 Rh5BG515500 Rh5CG539300 Rh5DG528200
rosa_wichuraiana Rw4G000930 Rw4G001980 Rw4G001990 Rw4G011950 Rw5G045850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1198, 2135
Acc36I ACCTGC 2 cut(s) 2090, 3445
AccB1I GGYRCC 4 cut(s) 226, 523, 3742, 3998
AccB7I CCANNNNNTGG 2 cut(s) 554, 1747
AccBSI CCGCTC 1 cut(s) 530
AccI GTMKAC 1 cut(s) 500
AccII CGCG 1 cut(s) 1842
AclWI GGATC 5 cut(s) 692, 705, 1616, 3746, 3759
AcoI YGGCCR 3 cut(s) 1312, 1749, 2895
AcsI RAATTY 9 cut(s) 65, 436, 1610, 1655, 1903, 2060, 2773, 3155, 3357
AcuI CTGAAG 5 cut(s) 939, 1878, 2361, 2853, 3380
AcyI GRCGYC 2 cut(s) 718, 3999
AflII CTTAAG 1 cut(s) 3607
AflIII ACRYGT 1 cut(s) 2184
AhdI GACNNNNNGTC 1 cut(s) 1224
AhlI ACTAGT 1 cut(s) 1994
AjnI CCWGG 4 cut(s) 2535, 2647, 2676, 3350
AjuI GAANNNNNNNTTGG 6 cut(s) 2220, 2252, 2994, 3026, 3900, 3932
Alw21I GWGCWC 4 cut(s) 448, 472, 820, 2265
AlwI GGATC 5 cut(s) 692, 705, 1616, 3746, 3759
AlwNI CAGNNNCTG 5 cut(s) 1747, 2810, 2876, 2923, 2975
Ama87I CYCGRG 3 cut(s) 1506, 2215, 2565
ApaI GGGCCC 1 cut(s) 204
ApoI RAATTY 9 cut(s) 65, 436, 1610, 1655, 1903, 2060, 2773, 3155, 3357
AseI ATTAAT 1 cut(s) 3579
Asp700I GAANNNNTTC 1 cut(s) 999
AspLEI GCGC 3 cut(s) 460, 3367, 4001
AsuC2I CCSGG 2 cut(s) 13, 4010
AsuNHI GCTAGC 1 cut(s) 1897
AvaI CYCGRG 3 cut(s) 1506, 2215, 2565
AvaII GGWCC 5 cut(s) 923, 1413, 2167, 3206, 3710
BaeGI GKGCMC 3 cut(s) 204, 528, 2581
BaeI ACNNNNGTAYC 4 cut(s) 3825, 3858, 4102, 4135
BalI TGGCCA 2 cut(s) 1751, 2897
BamHI GGATCC 2 cut(s) 697, 3751
BanI GGYRCC 4 cut(s) 226, 523, 3742, 3998
BanII GRGCYC 4 cut(s) 204, 434, 820, 3064
BarI GAAGNNNNNNTAC 2 cut(s) 3409, 3441
BauI CACGAG 2 cut(s) 1008, 3728
BbsI GAAGAC 2 cut(s) 2679, 3035
Bbv12I GWGCWC 4 cut(s) 448, 472, 820, 2265
BbvCI CCTCAGC 3 cut(s) 702, 855, 1417
BceAI ACGGC 4 cut(s) 240, 1327, 1907, 4055
BcgI CGANNNNNNTGC 2 cut(s) 550, 584
BciT130I CCWGG 4 cut(s) 2537, 2649, 2678, 3352
BciVI GTATCC 2 cut(s) 2899, 4130
BclI TGATCA 1 cut(s) 3132
BcnI CCSGG 2 cut(s) 13, 4010
BcuI ACTAGT 1 cut(s) 1994
BfaI CTAG 7 cut(s) 485, 876, 1898, 1995, 2256, 3414, 4101
BfmI CTRYAG 2 cut(s) 507, 2199
BfoI RGCGCY 2 cut(s) 461, 4002
BfrI CTTAAG 1 cut(s) 3607
BfuAI ACCTGC 2 cut(s) 2090, 3445
BfuI GTATCC 2 cut(s) 2899, 4130
BglII AGATCT 5 cut(s) 803, 1260, 1407, 2453, 2545
Bme1390I CCNGG 6 cut(s) 13, 2537, 2649, 2678, 3352, 4010
Bme18I GGWCC 5 cut(s) 923, 1413, 2167, 3206, 3710
BmeRI GACNNNNNGTC 1 cut(s) 1224
BmeT110I CYCGRG 3 cut(s) 1506, 2215, 2565
BmrFI CCNGG 6 cut(s) 13, 2537, 2649, 2678, 3352, 4010
BmrI ACTGGG 2 cut(s) 1440, 3155
BmtI GCTAGC 1 cut(s) 1901
BmuI ACTGGG 2 cut(s) 1440, 3155
BpiI GAAGAC 2 cut(s) 2679, 3035
BplI GAGNNNNNCTC 4 cut(s) 2443, 2475, 2462, 2494
BpmI CTGGAG 2 cut(s) 2685, 3216
Bpu10I CCTNAGC 4 cut(s) 702, 855, 1417, 3902
BpuEI CTTGAG 3 cut(s) 2466, 3392, 3655
BpuMI CCSGG 2 cut(s) 13, 4010
BsaAI YACGTR 1 cut(s) 4036
BsaBI GATNNNNATC 1 cut(s) 1867
BsaHI GRCGYC 2 cut(s) 718, 3999
BsaI GGTCTC 6 cut(s) 333, 1294, 2121, 3099, 3728, 4102
BsaJI CCNNGG 4 cut(s) 195, 2119, 2535, 3907
BsaXI ACNNNNNCTCC 4 cut(s) 2516, 2546, 3521, 3551
Bse118I RCCGGY 3 cut(s) 175, 407, 594
Bse3DI GCAATG 4 cut(s) 375, 966, 1647, 3961
Bse8I GATNNNNATC 1 cut(s) 1867
BseBI CCWGG 4 cut(s) 2537, 2649, 2678, 3352
BseDI CCNNGG 4 cut(s) 195, 2119, 2535, 3907
BseJI GATNNNNATC 1 cut(s) 1867
BseMI GCAATG 4 cut(s) 375, 966, 1647, 3961
BseRI GAGGAG 7 cut(s) 81, 148, 180, 1529, 3007, 3297, 3812
BseSI GKGCMC 3 cut(s) 204, 528, 2581
BseYI CCCAGC 2 cut(s) 572, 3522
BsgI GTGCAG 5 cut(s) 1968, 2862, 3642, 3696, 4055
Bsh1236I CGCG 1 cut(s) 1842
BshNI GGYRCC 4 cut(s) 226, 523, 3742, 3998
BsiHKAI GWGCWC 4 cut(s) 448, 472, 820, 2265
BsiHKCI CYCGRG 3 cut(s) 1506, 2215, 2565
BsiSI CCGG 5 cut(s) 12, 176, 408, 595, 4010
BsiWI CGTACG 1 cut(s) 4036
BslFI GGGAC 2 cut(s) 1119, 1705
BsmBI CGTCTC 1 cut(s) 2661
BsmFI GGGAC 2 cut(s) 1119, 1705
BsmI GAATGC 5 cut(s) 573, 1520, 1591, 2576, 3085
Bso31I GGTCTC 6 cut(s) 333, 1294, 2121, 3099, 3728, 4102
BsoBI CYCGRG 3 cut(s) 1506, 2215, 2565
Bsp120I GGGCCC 1 cut(s) 200
Bsp1286I GDGCHC 9 cut(s) 204, 434, 448, 472, 528, 820, 2265, 2581, 3064
Bsp1407I TGTACA 1 cut(s) 1600
Bsp19I CCATGG 1 cut(s) 2119
BspFNI CGCG 1 cut(s) 1842
BspMAI CTGCAG 1 cut(s) 2203
BspMI ACCTGC 2 cut(s) 2090, 3445
BspOI GCTAGC 1 cut(s) 1901
BspPI GGATC 5 cut(s) 692, 705, 1616, 3746, 3759
BspQI GCTCTTC 1 cut(s) 3399
BspT107I GGYRCC 4 cut(s) 226, 523, 3742, 3998
BspTI CTTAAG 1 cut(s) 3607
BspTNI GGTCTC 6 cut(s) 333, 1294, 2121, 3099, 3728, 4102
BsrBI CCGCTC 1 cut(s) 530
BsrDI GCAATG 4 cut(s) 375, 966, 1647, 3961
BsrFI RCCGGY 3 cut(s) 175, 407, 594
BsrGI TGTACA 1 cut(s) 1600
BssAI RCCGGY 3 cut(s) 175, 407, 594
BssECI CCNNGG 4 cut(s) 195, 2119, 2535, 3907
BssNI GRCGYC 2 cut(s) 718, 3999
BssSI CACGAG 2 cut(s) 1008, 3728
BssT1I CCWWGG 2 cut(s) 2119, 3907
Bst2BI CACGAG 2 cut(s) 1008, 3728
Bst2UI CCWGG 4 cut(s) 2537, 2649, 2678, 3352
Bst6I CTCTTC 4 cut(s) 1484, 2872, 3069, 3399
BstACI GRCGYC 2 cut(s) 718, 3999
BstAFI CTTAAG 1 cut(s) 3607
BstAUI TGTACA 1 cut(s) 1600
BstBAI YACGTR 1 cut(s) 4036
BstDSI CCRYGG 2 cut(s) 195, 2119
BstFNI CGCG 1 cut(s) 1842
BstH2I RGCGCY 2 cut(s) 461, 4002
BstHHI GCGC 3 cut(s) 460, 3367, 4001
BstNI CCWGG 4 cut(s) 2537, 2649, 2678, 3352
BstNSI RCATGY 4 cut(s) 61, 2188, 4085, 4139
BstSCI CCNGG 6 cut(s) 11, 2535, 2647, 2676, 3350, 4008
BstSFI CTRYAG 2 cut(s) 507, 2199
BstSLI GKGCMC 3 cut(s) 204, 528, 2581
BstUI CGCG 1 cut(s) 1842
BstV2I GAAGAC 2 cut(s) 2679, 3035
BstX2I RGATCY 7 cut(s) 697, 803, 1260, 1407, 2453, 2545, 3751
BstXI CCANNNNNNTGG 3 cut(s) 1228, 3256, 4156
BstYI RGATCY 7 cut(s) 697, 803, 1260, 1407, 2453, 2545, 3751
BsuI GTATCC 2 cut(s) 2899, 4130
BtgI CCRYGG 2 cut(s) 195, 2119
BtgZI GCGATG 2 cut(s) 1857, 4043
BtsI GCAGTG 3 cut(s) 792, 1892, 2208
BveI ACCTGC 2 cut(s) 2090, 3445
CaiI CAGNNNCTG 5 cut(s) 1747, 2810, 2876, 2923, 2975
CfoI GCGC 3 cut(s) 460, 3367, 4001
Cfr10I RCCGGY 3 cut(s) 175, 407, 594
CseI GACGC 3 cut(s) 726, 2691, 3059
DinI GGCGCC 1 cut(s) 4000
DraI TTTAAA 2 cut(s) 73, 2181
DrdI GACNNNNNNGTC 2 cut(s) 1198, 2135
DriI GACNNNNNGTC 1 cut(s) 1224
DseDI GACNNNNNNGTC 2 cut(s) 1198, 2135
EaeI YGGCCR 3 cut(s) 1312, 1749, 2895
Eam1104I CTCTTC 4 cut(s) 1484, 2872, 3069, 3399
Eam1105I GACNNNNNGTC 1 cut(s) 1224
EarI CTCTTC 4 cut(s) 1484, 2872, 3069, 3399
EciI GGCGGA 1 cut(s) 15
Ecl136II GAGCTC 1 cut(s) 818
Eco130I CCWWGG 2 cut(s) 2119, 3907
Eco24I GRGCYC 4 cut(s) 204, 434, 820, 3064
Eco31I GGTCTC 6 cut(s) 333, 1294, 2121, 3099, 3728, 4102
Eco32I GATATC 3 cut(s) 1865, 2089, 3949
Eco47I GGWCC 5 cut(s) 923, 1413, 2167, 3206, 3710
Eco53kI GAGCTC 1 cut(s) 818
Eco57I CTGAAG 5 cut(s) 939, 1878, 2361, 2853, 3380
Eco88I CYCGRG 3 cut(s) 1506, 2215, 2565
EcoICRI GAGCTC 1 cut(s) 818
EcoO109I RGGNCCY 3 cut(s) 200, 201, 2972
EcoRI GAATTC 3 cut(s) 436, 1903, 3155
EcoRII CCWGG 4 cut(s) 2535, 2647, 2676, 3350
EcoRV GATATC 3 cut(s) 1865, 2089, 3949
EcoT14I CCWWGG 2 cut(s) 2119, 3907
EcoT22I ATGCAT 1 cut(s) 4137
EcoT38I GRGCYC 4 cut(s) 204, 434, 820, 3064
EgeI GGCGCC 1 cut(s) 4000
EheI GGCGCC 1 cut(s) 4000
ErhI CCWWGG 2 cut(s) 2119, 3907
Esp3I CGTCTC 1 cut(s) 2661
FaqI GGGAC 2 cut(s) 1119, 1705
FauI CCCGC 9 cut(s) 124, 180, 222, 273, 354, 394, 535, 1643, 3988
FbaI TGATCA 1 cut(s) 3132
FblI GTMKAC 1 cut(s) 500
FriOI GRGCYC 4 cut(s) 204, 434, 820, 3064
FspBI CTAG 7 cut(s) 485, 876, 1898, 1995, 2256, 3414, 4101
GlaI GCGC 3 cut(s) 459, 3366, 4000
GsaI CCCAGC 2 cut(s) 576, 3526
GsuI CTGGAG 2 cut(s) 2685, 3216
HaeII RGCGCY 2 cut(s) 461, 4002
HapII CCGG 5 cut(s) 12, 176, 408, 595, 4010
HgaI GACGC 3 cut(s) 726, 2691, 3059
HhaI GCGC 3 cut(s) 460, 3367, 4001
Hin1I GRCGYC 2 cut(s) 718, 3999
Hin6I GCGC 3 cut(s) 458, 3365, 3999
HinP1I GCGC 3 cut(s) 458, 3365, 3999
HincII GTYRAC 5 cut(s) 501, 2155, 2401, 3325, 3632
HindII GTYRAC 5 cut(s) 501, 2155, 2401, 3325, 3632
HindIII AAGCTT 1 cut(s) 3423
HpaI GTTAAC 2 cut(s) 2401, 3325
HpaII CCGG 5 cut(s) 12, 176, 408, 595, 4010
HpyCH4IV ACGT 5 cut(s) 615, 633, 3485, 3634, 4035
HpySE526I ACGT 5 cut(s) 615, 633, 3485, 3634, 4035
Hsp92I GRCGYC 2 cut(s) 718, 3999
HspAI GCGC 3 cut(s) 458, 3365, 3999
KasI GGCGCC 1 cut(s) 3998
KroI GCCGGC 2 cut(s) 175, 594
KroNI GCCGGC 2 cut(s) 177, 596
Ksp22I TGATCA 1 cut(s) 3132
KspAI GTTAAC 2 cut(s) 2401, 3325
LguI GCTCTTC 1 cut(s) 3399
LmnI GCTCC 5 cut(s) 321, 475, 1111, 1351, 2537
MaeI CTAG 7 cut(s) 485, 876, 1898, 1995, 2256, 3414, 4101
MaeII ACGT 5 cut(s) 615, 633, 3485, 3634, 4035
MbiI CCGCTC 1 cut(s) 530
MfeI CAATTG 2 cut(s) 1848, 3308
MflI RGATCY 7 cut(s) 697, 803, 1260, 1407, 2453, 2545, 3751
MhlI GDGCHC 9 cut(s) 204, 434, 448, 472, 528, 820, 2265, 2581, 3064
MlsI TGGCCA 2 cut(s) 1751, 2897
MluNI TGGCCA 2 cut(s) 1751, 2897
Mly113I GGCGCC 1 cut(s) 3999
MmeI TCCRAC 8 cut(s) 379, 417, 964, 2169, 2218, 2315, 3150, 4147
Mox20I TGGCCA 2 cut(s) 1751, 2897
Mph1103I ATGCAT 1 cut(s) 4137
MroNI GCCGGC 2 cut(s) 175, 594
MroXI GAANNNNTTC 1 cut(s) 999
MscI TGGCCA 2 cut(s) 1751, 2897
MslI CAYNNNNRTG 5 cut(s) 776, 839, 1465, 4080, 4154
Msp20I TGGCCA 2 cut(s) 1751, 2897
MspA1I CMGCKG 3 cut(s) 1952, 3212, 3656
MspCI CTTAAG 1 cut(s) 3607
MspI CCGG 5 cut(s) 12, 176, 408, 595, 4010
MspR9I CCNGG 6 cut(s) 13, 2537, 2649, 2678, 3352, 4010
MunI CAATTG 2 cut(s) 1848, 3308
Mva1269I GAATGC 5 cut(s) 573, 1520, 1591, 2576, 3085
MvaI CCWGG 4 cut(s) 2537, 2649, 2678, 3352
MvnI CGCG 1 cut(s) 1842
NaeI GCCGGC 2 cut(s) 177, 596
NarI GGCGCC 1 cut(s) 3999
NciI CCSGG 2 cut(s) 13, 4010
NcoI CCATGG 1 cut(s) 2119
NgoMIV GCCGGC 2 cut(s) 175, 594
NheI GCTAGC 1 cut(s) 1897
NmeAIII GCCGAG 1 cut(s) 2593
NsiI ATGCAT 1 cut(s) 4137
NspI RCATGY 4 cut(s) 61, 2188, 4085, 4139
PaeR7I CTCGAG 1 cut(s) 2215
PciI ACATGT 1 cut(s) 2184
PciSI GCTCTTC 1 cut(s) 3399
PctI GAATGC 5 cut(s) 573, 1520, 1591, 2576, 3085
PdiI GCCGGC 2 cut(s) 177, 596
PdmI GAANNNNTTC 1 cut(s) 999
PfeI GAWTC 9 cut(s) 16, 450, 544, 1457, 1826, 1964, 2828, 3269, 3889
Pfl23II CGTACG 1 cut(s) 4036
PflMI CCANNNNNTGG 2 cut(s) 554, 1747
PluTI GGCGCC 1 cut(s) 4002
Ppu21I YACGTR 1 cut(s) 4036
PscI ACATGT 1 cut(s) 2184
PshBI ATTAAT 1 cut(s) 3579
Psp124BI GAGCTC 1 cut(s) 820
Psp6I CCWGG 4 cut(s) 2535, 2647, 2676, 3350
PspFI CCCAGC 2 cut(s) 572, 3522
PspGI CCWGG 4 cut(s) 2535, 2647, 2676, 3350
PspLI CGTACG 1 cut(s) 4036
PspOMI GGGCCC 1 cut(s) 200
PspXI VCTCGAGB 1 cut(s) 2215
PstI CTGCAG 1 cut(s) 2203
PstNI CAGNNNCTG 5 cut(s) 1747, 2810, 2876, 2923, 2975
PsuI RGATCY 7 cut(s) 697, 803, 1260, 1407, 2453, 2545, 3751
PvuII CAGCTG 3 cut(s) 1952, 3212, 3656
RseI CAYNNNNRTG 5 cut(s) 776, 839, 1465, 4080, 4154
SacI GAGCTC 1 cut(s) 820
SalI GTCGAC 1 cut(s) 499
SapI GCTCTTC 1 cut(s) 3399
ScrFI CCNGG 6 cut(s) 13, 2537, 2649, 2678, 3352, 4010
SduI GDGCHC 9 cut(s) 204, 434, 448, 472, 528, 820, 2265, 2581, 3064
SfcI CTRYAG 2 cut(s) 507, 2199
SfoI GGCGCC 1 cut(s) 4000
Sfr274I CTCGAG 1 cut(s) 2215
SgrAI CRCCGGYG 1 cut(s) 407
SinI GGWCC 5 cut(s) 923, 1413, 2167, 3206, 3710
SlaI CTCGAG 1 cut(s) 2215
SmiMI CAYNNNNRTG 5 cut(s) 776, 839, 1465, 4080, 4154
SmlI CTYRAG 5 cut(s) 2215, 2481, 3407, 3607, 3670
SmoI CTYRAG 5 cut(s) 2215, 2481, 3407, 3607, 3670
SpeI ACTAGT 1 cut(s) 1994
SspDI GGCGCC 1 cut(s) 3998
SspMI CTAG 7 cut(s) 485, 876, 1898, 1995, 2256, 3414, 4101
SstI GAGCTC 1 cut(s) 820
StyD4I CCNGG 6 cut(s) 11, 2535, 2647, 2676, 3350, 4008
StyI CCWWGG 2 cut(s) 2119, 3907
TaiI ACGT 5 cut(s) 618, 636, 3488, 3637, 4038
TaqII GACCGA 1 cut(s) 1208
TatI WGTACW 3 cut(s) 1066, 1600, 2812
TauI GCSGC 4 cut(s) 31, 34, 49, 3521
TfiI GAWTC 9 cut(s) 16, 450, 544, 1457, 1826, 1964, 2828, 3269, 3889
TspGWI ACGGA 2 cut(s) 2618, 4001
Van91I CCANNNNNTGG 2 cut(s) 554, 1747
Vha464I CTTAAG 1 cut(s) 3607
VpaK11BI GGWCC 5 cut(s) 923, 1413, 2167, 3206, 3710
VspI ATTAAT 1 cut(s) 3579
XapI RAATTY 9 cut(s) 65, 436, 1610, 1655, 1903, 2060, 2773, 3155, 3357
XbaI TCTAGA 1 cut(s) 484
XceI RCATGY 4 cut(s) 61, 2188, 4085, 4139
XcmI CCANNNNNNNNNTGG 2 cut(s) 1014, 2660
XhoI CTCGAG 1 cut(s) 2215
XmiI GTMKAC 1 cut(s) 500
XmnI GAANNNNTTC 1 cut(s) 999
XspI CTAG 7 cut(s) 485, 876, 1898, 1995, 2256, 3414, 4101
Zsp2I ATGCAT 1 cut(s) 4137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.