Prupe.6G032100_v2.0.a1

Enhancer of mRNA-decapping protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0000324
Physical Location & Seq
Forward (+)
2466890 .. 2474737
7848 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G032100.1

Sequence Viewer

Length: 4278 bp
ATGGCTTCTTCTGGGAATCCAAATCCACCGCCCCAGCAGCAACCCCAACCCTTTGACATGCAGAAATTCTTTAAACCCACAAACCCAACGCCACTCTCGCCCCAATCCCAAACCCAGAACAATTCAAACCAAAACCCTAACCCTAATTTGAGTTCCTCTTCGTTCCCAATCCCACCCGCCTCTTTCCCCCCGCCCTCTGGCCCTTACTCGTACCCTCCCCAGACGGCACCGTTTCACCACCACCAATTCCAATACCACCCTCACCCTCAGGCCCACCCCCACAGTAACCATCCCCAAATCCCTTACTCTCCTCAAGACCACCACTTGCTCCACCAGAGGTCTCTCTCATTTCCCACGCCACCCCTCCAACCCCCCTCTACTTACAACATTGCCACGGCCCCTTCCAACCCTAGCAACAACAACAGTAGCACCTCCAATTCGAACCCCAATTCGGGTGCTCGAATCATGGCGCTTCTCGGCGCTCCATCTGGCAATCTAGAGTTGTCGGCTGCTCAGCCCGAGTTATCCGTCCCCCCAGGGGTCTCGATCGTGCCCACTATCCCTATGGGGATTCCACCCACTGGCCCCACTCGAATGCCGAGCAATAAGCTCCCTAAAGGCAGGCATTTGATTGGTGATAATGTGGTTTACGATGTGGATGTTAGGTTGCAGGGTGAGTTTCAGCCACAGCTTGAAGTTACGCCGATTACCAAGTACGGTTCGGATCCTCAGCTTGTGCTGGGCCGCCAAATTGCTGTTAATAAGTCGTACATATGCTATGGATTGAAGCAGGGAAACATTCGGGTTTTGAATATACACACTGCATTGAGATCTTTGTTTCGAGCTCATACACAGAGGGTCACAGACATGGCTTTCTTTGCTGAAGATGTTCACCTTTTGGCTAGTGTGAGTGTAGAAGGACGGCTTTTTGTGTGGAAGATTTCTGAAGGTCCAGATGAAGAAGGTACACCACAAATCACAGGAAAGGTTGTTATTGCCATTCAAATAGTTGGAGAGGGGGAAGCTGTTCATCCACGAGTTTGTTGGCACTGTCATAAACAAGAGGTTTTGGTAGTTGGGTTTGGAAAGCGTGTATTAAGAATTGATACTACTAAAGTCGTGAAGGGTGAAGTCCCTTCTGCAGATGAGCCTCTCAAGTGTCCTGTTGAAAAGCTGATTGATGGAGTCCAATTTGTTGGTAAACATGATGGAGAAGTGACTGATTTGTCAATGTGCCAATGGATGACCACTCGTCTGGTTTCTGCTTCTATGGATGGAACAATAAAGATCTGGGAAGATCGCAAGGCACAACCACTTTTAGTATTGAGACCCTATGACGGCCATCCTGTTTATTCAGCTACATTTGTGACAGCTCCCCACCGGCCAGATCATATTATACTTATCACAGTGGGGCCACTTAATCGGGAAGTGAAGATCTGGTCCTCAGCGAGTGAAGAAGGCTGGCTACTTCCCAGTGATGCTGAGTCATGGAAATGTACTCAGACATTAGAGCTGAAGAGTTCTGCTGAACCCCGAGTTGAGGAGGCATTCTTTAATCAAGTCATAGCTTTGTCACAGGCTGGCCTTCTCTTACTTGCAAATGCTAAGAAGAATGCTATTTATGCTGTGCACTTAGAATTTGGTCCTGATCCAGCAGCAACGCGTATGGATTATATTGCAGAATTTACTGTTACCATGCCTATTTTGAGTTTTACTGGGACAAGTATTTCACCTCATGGTGAACAGATTGTCCAGGTTTATTGTGTCCAGACACTAGCCATTCAGCAGTATGCTCTGGAATTATCCAAGTGCTTGCCTCCACCTCTGGATAATGTGGGTTTAGAAAAGTCAGATTCCAATATTTCACGTGAACCATCAGGAGCTGAAGGATTTGCTTTGGATCTATCTGGAAGTAAACCCACAGAGATGCTGCTAGCTAATTCAAATTCAGCACTTAAACAAACCATACAAGATAGTAGTTCTGAGGGTGCAGTTTCCATGAGGTATCCTGTAAGCTCTAGTTCTGTTGAGGCAACCACTTCGAAAGATATTACCACTTCAAGTACAGAATCAAGACCTGTTGCAATGGCATCAGCTACTAGTGATTCAGATGTTGTTTTTGTTGCATCACCGCCTATTCCCTTGAGTCCTAGGTTGTCTAGGAAACTTTCTGGTTTAAGAAGTCCAACAGATGGCTCTGACCCGGGTCGCACACTCAATGAGCATGGTGGAGACCAACAAGTCAATGATTACTCAGTTGACAGGCAATTGGACAGTGTTCGTTCAAACTTGTCAGATGTGCCTGCAGTGGATGATGATTCAAGAAATATTGAGCAGAAAGTTGGACAAGATGATTTATCTAGTGTGCTCAATTCTCCTATCATGTTCAAACATCCAACCCATCTTATAACTCCCTCTGAGATTTTAATGGCTGCTTCATCTTCTGAAGGTACTAATCCTATTGACAGCAAGAATGAAGGGGAGGCAAATATCCAAGATGTGGTTGTTAACAGTGATATGGGTAATGCTGAGGTGGAGATTAAAGTAGTGGGTGAAGCAAGATCTACTCAAAATGATGAATTTGGATCTCAAGGAGAACCTCAAAATGTTATTTCAGAAAATAAGGAAAAATTCTTTTGCTCCCAGGCATCAGATCTTGGAATTGAGATGGCCCGAGAATGCTGTGCAATATCAGCCGAAACATATACTACAGATGAAGCTCGGCAAGTTGATGATTCCAGCATGACAGAACCACTGGCCCAGTCTAATGCTGGAGACGAAGACCAGGAATCTGCAAAAGATGCATCTGGGCCATGTACCACCCCACCAGTTTTTCAATCGCATACACAAACTACAAAAGTGAAAAAACAGAAGTGGAAAAACTCTCAAGCATCAGGTCAATCTTCCCCATCACCAAGTGTGCTCAATTCAATAGATTCCATCAATGAACCAGGAGGGAGTTCAAGCCCCCCCTCTGCAGAAGCTGCATTTCCTCAAATTATGGCCATGCAGGATACGATTAATCAGCTACTGACTATGCAGAAAGAACTGCAAAAGCAGATGACAATGATGGTTGCAGTCCCAGTTACAAAGGAGGGGAGAAGATTAGAGGCAGCTCTTGGGCGGAGTATGGAGAAGGCTGTAAAGGCCAATAATGATGCTTTATGGGCTCGTTTTCAAGAAGAGAATGCAAAGAATGAGAAGTTATTGCGAGACCGTAATCAGCAAATAACTAGCTTGATCAATAACTTCATGAACAAGGACTTTCCAGTCATGTTAGAGAAAATGGTGAAGAAGGAATTGGCGGTCGTTGGACCAGCTGTTGCTCGTGCTATAACTCCAGCTATTGAGAAAGCAATACCTCCAGCTATTTCTGATTCCTTCCAGAGAGGAGTTGGTGATAAGGCAGTTAATCAATTGGAAAAATCGGTTAACTCAAAACTTGAAGCAACCGTTTCCAGGCAAATCCAGGCGCAGTTCCAAACTTCTGGCAAACAAGCTCTTCAGGATGCTCTTAAGTCTAGTATGGAAGCTTCAGTGGTACCTGCATTTGAGAAGTCATGTAAGGCCATGTTTGAGCAAGTAGATGCTACGTTCCAGAAAGGAATGTTAGAACATACAAATGCGGCACAACAACACTTTGATTCTGCACATTCGCCACTGGCACTTGCTTTAAGGGAAGCTATTAGTTCTGCATCATCGGTGACTCAAACCTTAAGTGGCGAAGTGGCTGATGGTCAACGTAAGCTAATAGCTCTTGCAGCTGCACGAACAAGCTCAAGTGCAGTAAATCCCCTGGTCACACAACTAACCAATGGACCTTTGGGTGGTCTCCATGAGAAGGTTGAGGTGCCTTTGGATCCAACGAAGGAGCTGTCTAGGTTGGTTTCTGAACGGAAGTATGAGGAGGCTTTCACTGGAGCTCTACAAAGAAGTGATGTGACCATCGTATCGTGGTTGTGCTCTCAGGTTGATCTGCATGGGGTATTGTTGCTGAATCCTCTTCCTCTTAGCCAAGGGGTGCTTCTCTCTCTTTTGCAACAGCTGGCCTGTGATATCAGCAATGATACATCGAGAAAAGTTGCGTGGATGACAGATGTGGCGGCTGCCATAAACCCAGTAAACCAAATGATTGCAGTGCATGTGCGGCCCGTCTTTGAGCAAGTTTACCAGATACTGCACCATCAACATAGCTTGCCTACAATCTCTAGTGCCGAGCACACAAGTATTAGACTTCTGATGCATGTTATCAACTCCATGCTGATGGCCTGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000932 GO:0000956 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009892 GO:0009933 GO:0009965 GO:0009987 GO:0010014 GO:0010015 GO:0010016 GO:0010033 GO:0010071 GO:0010072 GO:0010154 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0022414 GO:0022622 GO:0023052 GO:0031086 GO:0031087 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0042221 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046700 GO:0046983 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048532 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071704 GO:0090304 GO:0090305 GO:0090421 GO:0097159 GO:0099402 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
Ge1_WD40

Protein Analysis

1426

Amino Acids

154.72

Weight (kDa)

5.66

Isoelectric Point (pI)

47.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13290 AT3G13300 AT3G13300 AT3G13300
fragaria_vesca FvH4_3g41972 FvH4_3g41980 FvH4_3g41980 FvH4_3g41980 FvH4_3g41980 FvH4_3g42780 FvH4_3g42790 FvH4_3g42820 FvH4_3g42820 FvH4_3g42820 FvH4_3g42820 FvH4_4g01881 FvH4_7g10420
malus_domestica MD00G1013700.v1.1 MD03G1039800.v1.1 MD11G1041300.v1.1 MD11G1094900.v1.1
prunus_persica Prupe.6G032100_v2.0.a1
pyrus_communis pycom03g03070 pycom11g03500 pycom11g08470 pycom12g06730
rosa_chinensis RchiOBHm_Chr1g0328831 RchiOBHm_Chr4g0386471 RchiOBHm_Chr4g0386481 RchiOBHm_Chr4g0386551 RchiOBHm_Chr4g0386701 RchiOBHm_Chr4g0386801 RchiOBHm_Chr4g0386811 RchiOBHm_Chr4g0386821 RchiOBHm_Chr4g0386921 RchiOBHm_Chr4g0386931 RchiOBHm_Chr4g0386941 RchiOBHm_Chr4g0387211 RchiOBHm_Chr4g0389101 RchiOBHm_Chr4g0389111 RchiOBHm_Chr4g0389201 RchiOBHm_Chr4g0389211 RchiOBHm_Chr4g0389321 RchiOBHm_Chr4g0389331 RchiOBHm_Chr4g0389341 RchiOBHm_Chr4g0389411 RchiOBHm_Chr5g0075171
rosa_laevigata RLG00000009985 RLG00000009990 RLG00000009996 RLG00000009998 RLG00000010136 RLG00000010140 RLG00000036537
rosa_multiflora Rmu_co8461959.1_g000001 Rmu_sc0000013.1_g000017 Rmu_sc0000013.1_g000024 Rmu_sc0001015.1_g000001 Rmu_sc0001395.1_g000004 Rmu_sc0001942.1_g000022 Rmu_sc0002498.1_g000018 Rmu_sc0002498.1_g000020 Rmu_sc0002557.1_g000005 Rmu_sc0002604.1_g000010 Rmu_sc0002604.1_g000011 Rmu_sc0004750.1_g000003 Rmu_sc0006571.1_g000001 Rmu_sc0006571.1_g000014 Rmu_sc0006571.1_g000018 Rmu_sc0008050.1_g000016 Rmu_sc0008066.1_g000009 Rmu_sc0008380.1_g000006 Rmu_sc0008380.1_g000010 Rmu_sc0008380.1_g000012 Rmu_sc0010272.1_g000021 Rmu_sc0010693.1_g000010 Rmu_sc0010693.1_g000012 Rmu_sc0010693.1_g000014 Rmu_sc0010693.1_g000015 Rmu_sc0031790.1_g000003 Rmu_sc0040539.1_g000001 Rmu_ssc0000211.1_g000001 Rmu_ssc0000211.1_g000011 Rmu_ssc0000211.1_g000013
rosa_roxburghii Rroxscaffold_1G00006420 Rroxscaffold_1G00006430 Rroxscaffold_5G00334020 Rroxscaffold_5G00334050 Rroxscaffold_5G00335420
rosa_rugosa Rorug03G0307600 Rorug03G0308600 Rorug03G0322400 Rorug05G0437200 Rorug05G0437200 Rorug05G0437200
rosa_samantha Rh1BG075700 Rh4BG010000 Rh4BG010100 Rh4BG010200 Rh4BG020000 Rh4BG020400 Rh4BG172100 Rh4CG015300 Rh4CG016300 Rh5AG494100 Rh5AG494200 Rh5BG515500 Rh5CG539300 Rh5DG528200
rosa_wichuraiana Rw4G000930 Rw4G001980 Rw4G001990 Rw4G011950 Rw5G045850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2408
AasI GACNNNNNNGTC 3 cut(s) 1225, 2240, 3269
Acc36I ACCTGC 1 cut(s) 3553
Acc65I GGTACC 1 cut(s) 3541
AccB1I GGYRCC 3 cut(s) 226, 3541, 3850
AccB7I CCANNNNNTGG 3 cut(s) 581, 2192, 2500
AccII CGCG 1 cut(s) 1663
AclWI GGATC 7 cut(s) 719, 732, 1643, 1908, 2593, 3854, 3867
AcoI YGGCCR 3 cut(s) 1339, 1382, 3003
AcsI RAATTY 6 cut(s) 65, 1637, 1682, 1945, 2579, 2630
AcuI CTGAAG 7 cut(s) 903, 966, 1535, 1905, 2466, 3488, 3519
AcvI CACGTG 1 cut(s) 1868
AdeI CACNNNGTG 1 cut(s) 2918
AfaI GTAC 9 cut(s) 212, 716, 770, 967, 1498, 2065, 2452, 2818, 3543
AflII CTTAAG 2 cut(s) 3515, 3715
AflIII ACRYGT 1 cut(s) 1661
AhdI GACNNNNNGTC 1 cut(s) 1251
AhlI ACTAGT 1 cut(s) 2099
AjnI CCWGG 8 cut(s) 535, 1752, 2643, 2784, 2950, 3458, 3468, 3795
AjuI GAANNNNNNNTTGG 4 cut(s) 3284, 3316, 4008, 4040
AloI GAACNNNNNNTCC 2 cut(s) 1734, 1766
Alw21I GWGCWC 8 cut(s) 460, 847, 1632, 2370, 2925, 3925, 3965, 4221
Alw26I GTCTC 7 cut(s) 345, 547, 1321, 2226, 2769, 3207, 3836
Alw44I GTGCAC 1 cut(s) 1628
AlwI GGATC 7 cut(s) 719, 732, 1643, 1908, 2593, 3854, 3867
AlwNI CAGNNNCTG 4 cut(s) 1883, 2984, 3031, 4177
Ama87I CYCGRG 4 cut(s) 518, 1533, 2203, 2673
ApaLI GTGCAC 1 cut(s) 1628
ApoI RAATTY 6 cut(s) 65, 1637, 1682, 1945, 2579, 2630
ArsI GACNNNNNNTTYG 2 cut(s) 3860, 3892
AseI ATTAAT 1 cut(s) 3021
Asp700I GAANNNNTTC 2 cut(s) 888, 1026
Asp718I GGTACC 1 cut(s) 3541
AspA2I CCTAGG 1 cut(s) 2150
AspLEI GCGC 3 cut(s) 472, 482, 3475
AsuC2I CCSGG 2 cut(s) 2204, 2205
AsuII TTCGAA 2 cut(s) 440, 2042
AsuNHI GCTAGC 1 cut(s) 1933
AvaI CYCGRG 4 cut(s) 518, 1533, 2203, 2673
AvaII GGWCC 5 cut(s) 950, 1440, 1643, 3314, 3818
AvrII CCTAGG 1 cut(s) 2150
AxyI CCTNAGG 1 cut(s) 267
BaeGI GKGCMC 2 cut(s) 555, 1632
BaeI ACNNNNGTAYC 2 cut(s) 3933, 3966
BalI TGGCCA 1 cut(s) 3005
BamHI GGATCC 2 cut(s) 724, 3859
BanI GGYRCC 3 cut(s) 226, 3541, 3850
BanII GRGCYC 3 cut(s) 847, 3172, 3925
BarI GAAGNNNNNNTAC 4 cut(s) 1449, 1481, 3517, 3549
BauI CACGAG 2 cut(s) 1035, 3327
BbrPI CACGTG 1 cut(s) 1868
BbsI GAAGAC 1 cut(s) 2787
Bbv12I GWGCWC 8 cut(s) 460, 847, 1632, 2370, 2925, 3925, 3965, 4221
BbvCI CCTCAGC 3 cut(s) 729, 1444, 2529
BceAI ACGGC 4 cut(s) 240, 411, 938, 1354
BcgI CGANNNNNNTGC 2 cut(s) 2022, 2056
BciT130I CCWGG 8 cut(s) 537, 1754, 2645, 2786, 2952, 3460, 3470, 3797
BciVI GTATCC 2 cut(s) 2016, 3007
BclI TGATCA 1 cut(s) 3240
BcnI CCSGG 2 cut(s) 2204, 2205
BcoDI GTCTC 7 cut(s) 345, 547, 1321, 2226, 2769, 3207, 3836
BcuI ACTAGT 1 cut(s) 2099
BfmI CTRYAG 4 cut(s) 1140, 2304, 2709, 2976
BfoI RGCGCY 2 cut(s) 473, 483
BfrI CTTAAG 2 cut(s) 3515, 3715
BfuAI ACCTGC 1 cut(s) 3553
BfuI GTATCC 2 cut(s) 2016, 3007
BglII AGATCT 5 cut(s) 830, 1287, 1434, 2561, 2653
BlnI CCTAGG 1 cut(s) 2150
BlpI GCTNAGC 1 cut(s) 513
Bme18I GGWCC 5 cut(s) 950, 1440, 1643, 3314, 3818
BmeRI GACNNNNNGTC 1 cut(s) 1251
BmeT110I CYCGRG 4 cut(s) 518, 1533, 2203, 2673
BmiI GGNNCC 8 cut(s) 228, 399, 586, 726, 1413, 3543, 3852, 3861
BmrI ACTGGG 5 cut(s) 1467, 1725, 2755, 3077, 4112
BmtI GCTAGC 1 cut(s) 1937
BmuI ACTGGG 5 cut(s) 1467, 1725, 2755, 3077, 4112
BoxI GACNNNNGTC 1 cut(s) 2205
BpiI GAAGAC 1 cut(s) 2787
BpmI CTGGAG 4 cut(s) 2793, 3324, 3348, 3939
Bpu10I CCTNAGC 3 cut(s) 729, 1444, 2529
Bpu1102I GCTNAGC 1 cut(s) 513
Bpu14I TTCGAA 2 cut(s) 440, 2042
BpuEI CTTGAG 6 cut(s) 297, 1139, 2164, 2574, 2871, 3763
BpuMI CCSGG 2 cut(s) 2204, 2205
BsaAI YACGTR 1 cut(s) 1868
BsaI GGTCTC 6 cut(s) 345, 547, 1321, 2226, 3207, 3836
BsaJI CCNNGG 8 cut(s) 393, 535, 536, 2150, 2203, 2643, 3795, 4015
Bse118I RCCGGY 1 cut(s) 1380
Bse21I CCTNAGG 1 cut(s) 267
Bse3DI GCAATG 3 cut(s) 387, 2091, 4069
BseBI CCWGG 8 cut(s) 537, 1754, 2645, 2786, 2952, 3460, 3470, 3797
BseDI CCNNGG 8 cut(s) 393, 535, 536, 2150, 2203, 2643, 3795, 4015
BseMI GCAATG 3 cut(s) 387, 2091, 4069
BseRI GAGGAG 4 cut(s) 300, 1556, 3405, 3920
BseSI GKGCMC 2 cut(s) 555, 1632
BseYI CCCAGC 2 cut(s) 33, 739
BsgI GTGCAG 5 cut(s) 2010, 3633, 3750, 3804, 4163
Bsh1236I CGCG 1 cut(s) 1663
Bsh1285I CGRYCG 2 cut(s) 549, 3309
BshNI GGYRCC 3 cut(s) 226, 3541, 3850
BsiEI CGRYCG 2 cut(s) 549, 3309
BsiHKAI GWGCWC 8 cut(s) 460, 847, 1632, 2370, 2925, 3925, 3965, 4221
BsiHKCI CYCGRG 4 cut(s) 518, 1533, 2203, 2673
BsiSI CCGG 2 cut(s) 1381, 2204
BslFI GGGAC 4 cut(s) 515, 1118, 1732, 3065
BsmAI GTCTC 7 cut(s) 345, 547, 1321, 2226, 2769, 3207, 3836
BsmBI CGTCTC 1 cut(s) 2769
BsmFI GGGAC 4 cut(s) 515, 1118, 1732, 3065
BsmI GAATGC 5 cut(s) 600, 1547, 1618, 2684, 3193
Bso31I GGTCTC 6 cut(s) 345, 547, 1321, 2226, 3207, 3836
BsoBI CYCGRG 4 cut(s) 518, 1533, 2203, 2673
Bsp119I TTCGAA 2 cut(s) 440, 2042
Bsp1720I GCTNAGC 1 cut(s) 513
BspFNI CGCG 1 cut(s) 1663
BspHI TCATGA 1 cut(s) 3252
BspLI GGNNCC 8 cut(s) 228, 399, 586, 726, 1413, 3543, 3852, 3861
BspMAI CTGCAG 3 cut(s) 1144, 2308, 2980
BspMI ACCTGC 1 cut(s) 3553
BspOI GCTAGC 1 cut(s) 1937
BspPI GGATC 7 cut(s) 719, 732, 1643, 1908, 2593, 3854, 3867
BspQI GCTCTTC 1 cut(s) 3507
BspT104I TTCGAA 2 cut(s) 440, 2042
BspT107I GGYRCC 3 cut(s) 226, 3541, 3850
BspTI CTTAAG 2 cut(s) 3515, 3715
BspTNI GGTCTC 6 cut(s) 345, 547, 1321, 2226, 3207, 3836
BsrDI GCAATG 3 cut(s) 387, 2091, 4069
BsrFI RCCGGY 1 cut(s) 1380
BssAI RCCGGY 1 cut(s) 1380
BssECI CCNNGG 8 cut(s) 393, 535, 536, 2150, 2203, 2643, 3795, 4015
BssSI CACGAG 2 cut(s) 1035, 3327
BssT1I CCWWGG 2 cut(s) 2150, 4015
Bst2BI CACGAG 2 cut(s) 1035, 3327
Bst2UI CCWGG 8 cut(s) 537, 1754, 2645, 2786, 2952, 3460, 3470, 3797
Bst6I CTCTTC 5 cut(s) 163, 1511, 3177, 3507, 4008
BstAFI CTTAAG 2 cut(s) 3515, 3715
BstAPI GCANNNNNTGC 2 cut(s) 2801, 2984
BstBAI YACGTR 1 cut(s) 1868
BstBI TTCGAA 2 cut(s) 440, 2042
BstC8I GCNNGC 8 cut(s) 623, 1463, 1582, 1814, 1935, 2304, 4047, 4196
BstDSI CCRYGG 1 cut(s) 393
BstFNI CGCG 1 cut(s) 1663
BstH2I RGCGCY 2 cut(s) 473, 483
BstHHI GCGC 3 cut(s) 472, 482, 3475
BstMAI GTCTC 7 cut(s) 345, 547, 1321, 2226, 2769, 3207, 3836
BstMCI CGRYCG 2 cut(s) 549, 3309
BstNI CCWGG 8 cut(s) 537, 1754, 2645, 2786, 2952, 3460, 3470, 3797
BstNSI RCATGY 3 cut(s) 61, 4145, 4247
BstPAI GACNNNNGTC 1 cut(s) 2205
BstSFI CTRYAG 4 cut(s) 1140, 2304, 2709, 2976
BstSLI GKGCMC 2 cut(s) 555, 1632
BstUI CGCG 1 cut(s) 1663
BstV2I GAAGAC 1 cut(s) 2787
BstX2I RGATCY 9 cut(s) 724, 830, 1287, 1434, 1900, 2561, 2585, 2653, 3859
BstXI CCANNNNNNTGG 4 cut(s) 1196, 1255, 3488, 4264
BstYI RGATCY 9 cut(s) 724, 830, 1287, 1434, 1900, 2561, 2585, 2653, 3859
Bsu36I CCTNAGG 1 cut(s) 267
BsuI GTATCC 2 cut(s) 2016, 3007
BtgI CCRYGG 1 cut(s) 393
BtsI GCAGTG 3 cut(s) 819, 2313, 4143
BveI ACCTGC 1 cut(s) 3553
Cac8I GCNNGC 8 cut(s) 623, 1463, 1582, 1814, 1935, 2304, 4047, 4196
CaiI CAGNNNCTG 4 cut(s) 1883, 2984, 3031, 4177
CciI TCATGA 1 cut(s) 3252
CfoI GCGC 3 cut(s) 472, 482, 3475
Cfr10I RCCGGY 1 cut(s) 1380
Cfr9I CCCGGG 1 cut(s) 2203
Csp6I GTAC 9 cut(s) 211, 715, 769, 966, 1497, 2064, 2451, 2817, 3542
CviQI GTAC 9 cut(s) 211, 715, 769, 966, 1497, 2064, 2451, 2817, 3542
DraI TTTAAA 1 cut(s) 73
DraIII CACNNNGTG 1 cut(s) 2918
DrdI GACNNNNNNGTC 3 cut(s) 1225, 2240, 3269
DriI GACNNNNNGTC 1 cut(s) 1251
DseDI GACNNNNNNGTC 3 cut(s) 1225, 2240, 3269
EaeI YGGCCR 3 cut(s) 1339, 1382, 3003
Eam1104I CTCTTC 5 cut(s) 163, 1511, 3177, 3507, 4008
Eam1105I GACNNNNNGTC 1 cut(s) 1251
EarI CTCTTC 5 cut(s) 163, 1511, 3177, 3507, 4008
EciI GGCGGA 1 cut(s) 3139
Ecl136II GAGCTC 2 cut(s) 845, 3923
Eco130I CCWWGG 2 cut(s) 2150, 4015
Eco24I GRGCYC 3 cut(s) 847, 3172, 3925
Eco31I GGTCTC 6 cut(s) 345, 547, 1321, 2226, 3207, 3836
Eco32I GATATC 1 cut(s) 4057
Eco47I GGWCC 5 cut(s) 950, 1440, 1643, 3314, 3818
Eco53kI GAGCTC 2 cut(s) 845, 3923
Eco57I CTGAAG 7 cut(s) 903, 966, 1535, 1905, 2466, 3488, 3519
Eco72I CACGTG 1 cut(s) 1868
Eco81I CCTNAGG 1 cut(s) 267
Eco88I CYCGRG 4 cut(s) 518, 1533, 2203, 2673
EcoICRI GAGCTC 2 cut(s) 845, 3923
EcoRII CCWGG 8 cut(s) 535, 1752, 2643, 2784, 2950, 3458, 3468, 3795
EcoRV GATATC 1 cut(s) 4057
EcoT14I CCWWGG 2 cut(s) 2150, 4015
EcoT22I ATGCAT 2 cut(s) 2806, 4245
EcoT38I GRGCYC 3 cut(s) 847, 3172, 3925
ErhI CCWWGG 2 cut(s) 2150, 4015
Esp3I CGTCTC 1 cut(s) 2769
FalI AAGNNNNNCTT 4 cut(s) 909, 941, 4008, 4040
FaqI GGGAC 4 cut(s) 515, 1118, 1732, 3065
FauI CCCGC 2 cut(s) 184, 198
FauNDI CATATG 1 cut(s) 773
FbaI TGATCA 1 cut(s) 3240
FriOI GRGCYC 3 cut(s) 847, 3172, 3925
GlaI GCGC 3 cut(s) 471, 481, 3474
GsaI CCCAGC 2 cut(s) 37, 743
GsuI CTGGAG 4 cut(s) 2793, 3324, 3348, 3939
HaeII RGCGCY 2 cut(s) 473, 483
HapII CCGG 2 cut(s) 1381, 2204
HhaI GCGC 3 cut(s) 472, 482, 3475
Hin6I GCGC 3 cut(s) 470, 480, 3473
HinP1I GCGC 3 cut(s) 470, 480, 3473
HincII GTYRAC 4 cut(s) 2260, 2509, 3433, 3740
HindII GTYRAC 4 cut(s) 2260, 2509, 3433, 3740
HindIII AAGCTT 1 cut(s) 3531
HpaI GTTAAC 2 cut(s) 2509, 3433
HpaII CCGG 2 cut(s) 1381, 2204
HpyCH4IV ACGT 3 cut(s) 1867, 3593, 3742
HpySE526I ACGT 3 cut(s) 1867, 3593, 3742
HspAI GCGC 3 cut(s) 470, 480, 3473
KpnI GGTACC 1 cut(s) 3545
Ksp22I TGATCA 1 cut(s) 3240
KspAI GTTAAC 2 cut(s) 2509, 3433
LguI GCTCTTC 1 cut(s) 3507
LmnI GCTCC 8 cut(s) 333, 487, 615, 1378, 1880, 2645, 3871, 3920
MaeII ACGT 3 cut(s) 1867, 3593, 3742
MfeI CAATTG 2 cut(s) 2267, 3416
MflI RGATCY 9 cut(s) 724, 830, 1287, 1434, 1900, 2561, 2585, 2653, 3859
MlsI TGGCCA 1 cut(s) 3005
MluI ACGCGT 1 cut(s) 1661
MluNI TGGCCA 1 cut(s) 3005
MlyI GAGTC 4 cut(s) 1194, 1493, 2155, 3700
MmeI TCCRAC 8 cut(s) 391, 429, 991, 2210, 2323, 2420, 3292, 3887
Mox20I TGGCCA 1 cut(s) 3005
Mph1103I ATGCAT 2 cut(s) 2806, 4245
MroXI GAANNNNTTC 2 cut(s) 888, 1026
MscI TGGCCA 1 cut(s) 3005
MslI CAYNNNNRTG 6 cut(s) 593, 866, 1492, 1925, 3621, 4262
Msp20I TGGCCA 1 cut(s) 3005
MspA1I CMGCKG 3 cut(s) 3320, 3764, 4045
MspCI CTTAAG 2 cut(s) 3515, 3715
MspI CCGG 2 cut(s) 1381, 2204
MunI CAATTG 2 cut(s) 2267, 3416
Mva1269I GAATGC 5 cut(s) 600, 1547, 1618, 2684, 3193
MvaI CCWGG 8 cut(s) 537, 1754, 2645, 2786, 2952, 3460, 3470, 3797
MvnI CGCG 1 cut(s) 1663
NciI CCSGG 2 cut(s) 2204, 2205
NdeI CATATG 1 cut(s) 773
NheI GCTAGC 1 cut(s) 1933
NlaIV GGNNCC 8 cut(s) 228, 399, 586, 726, 1413, 3543, 3852, 3861
NmeAIII GCCGAG 4 cut(s) 456, 624, 2701, 4240
NmuCI GTSAC 7 cut(s) 859, 1216, 1366, 1572, 3703, 3799, 3940
NsiI ATGCAT 2 cut(s) 2806, 4245
NspI RCATGY 3 cut(s) 61, 4145, 4247
NspV TTCGAA 2 cut(s) 440, 2042
PagI TCATGA 1 cut(s) 3252
PasI CCCWGGG 1 cut(s) 536
PciSI GCTCTTC 1 cut(s) 3507
PctI GAATGC 5 cut(s) 600, 1547, 1618, 2684, 3193
PdmI GAANNNNTTC 2 cut(s) 888, 1026
PflMI CCANNNNNTGG 3 cut(s) 581, 2192, 2500
Ple19I CGATCG 1 cut(s) 549
PleI GAGTC 4 cut(s) 1193, 1492, 2154, 3700
PmaCI CACGTG 1 cut(s) 1868
PmlI CACGTG 1 cut(s) 1868
PpsI GAGTC 4 cut(s) 1193, 1492, 2154, 3700
Ppu21I YACGTR 1 cut(s) 1868
PshAI GACNNNNGTC 1 cut(s) 2205
PshBI ATTAAT 1 cut(s) 3021
PsiI TTATAA 1 cut(s) 2408
Psp124BI GAGCTC 2 cut(s) 847, 3925
Psp6I CCWGG 8 cut(s) 535, 1752, 2643, 2784, 2950, 3458, 3468, 3795
PspCI CACGTG 1 cut(s) 1868
PspFI CCCAGC 2 cut(s) 33, 739
PspGI CCWGG 8 cut(s) 535, 1752, 2643, 2784, 2950, 3458, 3468, 3795
PspN4I GGNNCC 8 cut(s) 228, 399, 586, 726, 1413, 3543, 3852, 3861
PstI CTGCAG 3 cut(s) 1144, 2308, 2980
PstNI CAGNNNCTG 4 cut(s) 1883, 2984, 3031, 4177
PsuI RGATCY 9 cut(s) 724, 830, 1287, 1434, 1900, 2561, 2585, 2653, 3859
PvuI CGATCG 1 cut(s) 549
PvuII CAGCTG 3 cut(s) 3320, 3764, 4045
RsaI GTAC 9 cut(s) 212, 716, 770, 967, 1498, 2065, 2452, 2818, 3543
RsaNI GTAC 9 cut(s) 211, 715, 769, 966, 1497, 2064, 2451, 2817, 3542
RseI CAYNNNNRTG 6 cut(s) 593, 866, 1492, 1925, 3621, 4262
SacI GAGCTC 2 cut(s) 847, 3925
SapI GCTCTTC 1 cut(s) 3507
SchI GAGTC 4 cut(s) 1194, 1493, 2155, 3700
SfcI CTRYAG 4 cut(s) 1140, 2304, 2709, 2976
SfuI TTCGAA 2 cut(s) 440, 2042
SinI GGWCC 5 cut(s) 950, 1440, 1643, 3314, 3818
SmaI CCCGGG 1 cut(s) 2205
SmiMI CAYNNNNRTG 6 cut(s) 593, 866, 1492, 1925, 3621, 4262
SmlI CTYRAG 8 cut(s) 312, 1154, 2143, 2589, 2886, 3515, 3715, 3778
SmoI CTYRAG 8 cut(s) 312, 1154, 2143, 2589, 2886, 3515, 3715, 3778
SpeI ACTAGT 1 cut(s) 2099
SspI AATATT 2 cut(s) 1861, 2329
SstI GAGCTC 2 cut(s) 847, 3925
StyI CCWWGG 2 cut(s) 2150, 4015
TaiI ACGT 3 cut(s) 1870, 3596, 3745
TaqI TCGA 7 cut(s) 440, 460, 545, 592, 841, 2042, 4073
TatI WGTACW 2 cut(s) 1496, 2063
TauI GCSGC 4 cut(s) 747, 3629, 4106, 4150
TseFI GTSAC 7 cut(s) 859, 1216, 1366, 1572, 3703, 3799, 3940
Tsp45I GTSAC 7 cut(s) 859, 1216, 1366, 1572, 3703, 3799, 3940
TspDTI ATGAA 9 cut(s) 972, 1019, 2427, 2490, 2592, 2730, 2961, 3241, 3269
TspGWI ACGGA 2 cut(s) 517, 3910
TspMI CCCGGG 1 cut(s) 2203
Van91I CCANNNNNTGG 3 cut(s) 581, 2192, 2500
Vha464I CTTAAG 2 cut(s) 3515, 3715
VneI GTGCAC 1 cut(s) 1628
VpaK11BI GGWCC 5 cut(s) 950, 1440, 1643, 3314, 3818
VspI ATTAAT 1 cut(s) 3021
XapI RAATTY 6 cut(s) 65, 1637, 1682, 1945, 2579, 2630
XbaI TCTAGA 1 cut(s) 496
XceI RCATGY 3 cut(s) 61, 4145, 4247
XcmI CCANNNNNNNNNTGG 5 cut(s) 562, 1041, 2768, 3392, 3820
XmaI CCCGGG 1 cut(s) 2203
XmaJI CCTAGG 1 cut(s) 2150
XmnI GAANNNNTTC 2 cut(s) 888, 1026
Zsp2I ATGCAT 2 cut(s) 2806, 4245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.