RLG00000036537

Enhancer of mRNA-decapping protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000324
Physical Location & Seq
Reverse (-)
81637398 .. 81644449
7052 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036537

Sequence Viewer

Length: 4194 bp
ATGGCGTCCCCCGGAAACCCCAATCCGCCGCCGCAGCAACAACCGCAGCCCTTCGACATGGCCAAGTTCTTTAAACCCACAAACCCAACCCCTCTCTCCCCCCATTCCCAGCCTCAAACCCAGGCCCCCACCCAAAACCCTAACCCTAATCTGAACTCCTCAGGTTTCCCAATCCCTCCGGCATCTTATCCTCCTCCCTCTGGGCCCTACTCCTACCCTCCCCAAACGGCGCCGTATCACCAGCAGCAATTCCAGTACCAGCACCACCCTCAGCCTCATCCTCACAACCACCCCCAAATTCCTTACTCTCAAGACCACCAGCTGCTTCAACAGCGGTCGCTCTCGTTTCCCATCCCGCCCCTGGCTCCCGGTAATTACAACATTGCCGCCTCCGCCGCCGCGGCCTCCTCCAACCCCGGAAACGCCACCACCACCAACCCCAATTCCGGCGCCCGAATCATGGCCCTGCTCGGTGCTCCTTCCTCCAGTCTAGAATTGGGGCCGCAGCCGGAGTTGCCAGTCCCCGCCGGGAGCGGGGTTTCGATGGTGCCCACTCTTCCTATGGGGATTCCACCCTCCCCGGCTCGAATGCCGAGCAATAAGCTGCCGAAGGGCAGGCATTTGATTGGGGATACTGTGGTTTACGATGTGGATGTTAGGTTGCCCGGGGAGTTTCAGCCGCAGCTGGAAGTTACCCCCATTACTAAGTATGGTTCGGATCCTCAGCTGGTGTTGGGCCGCCAAATCGCGGTTAATAAGTCGTATATATGCTATGGATTGAAGCAGGGGAATATTCGGGTTTTGAATATACACACTGCATTGAGATCTTTGTTTCGAGCTCACACACAGAGGGTCACAGACATGGCTTTCTTTGCCGAGGATGTTCACCTTTTGGCTAGTGTGAGTGTAGAAGGACGGCTTTTTGTGTGGAAAATTTCTGAAGGTCCAGATGAAGAAGGTACGCCACAAATCACAGGAAAGATTGTTGTTGCCATTCAAATAGTTGGTGAGGGGGAAGCTGTTCATCCACGAGTTTGTTGGCACTGTCATAAACAAGAGGTTTTGGTTGTTGGGATTGGGAAGCGTGTTCTACGAATTGATACTACTAAAGTTGCAAAGGGTGAAGTCCCTTCTGCTGAGGATCCTATTAAGTGTCCTGTTGAAAAGCTTGTTGATGGGGTCCAATTTGTTGGTAAACATGATGGAGAGGTGACAGATTTATCAATGTGCCAATGGATGACCACTCGGCTGGTTTCTGCTTCTATGGATGGAACTATAAAGATCTGGGAAGATCGCAAGTCACTACCACTTTTAGTACTGAGACCCTATGATGGCCTTCCTGTTTATTCGTCTACATTTGTGACAGCTCCCAACAGACCTGATCACATCATACTTATAACAGTGGGCCCACTTAATCGGGAAGTGAAGATCTGGTCTTCAGCGAGTGAAGAAGGCTGGCTGCTTCCCAGTGATGCTGAGTCATGGAAATGTACCCAGACCTTAGAATTAAAGAGCTCTGCTCAACCTCGAGTCGAGGATGCATTCTTTAATCAAGTCATAGCATTGTCACAAGCTGGCCTTCTTTTACTTGCAAATGCTAAGAAGAATGCTATATATGCTGTGCACTTAGACTTTGGTGGTGAGCCAGCATCCACACGCATGGATTACATAGCAGAATTTACTGTTACAATGCCTATTTTGAGCTTTACTGGGACAAGTATATCACCTCATGGTGAACAAATCGTTCAGGTGTATTGTGTCCAAACACAAGCCATTCAACAGTATGCTTTGGACTTATCCAAGTGCTTACCTCCCCCATTGGAGAATTCAGGTTTGGAAAAGTCAGATTCCAGTGTTTCACATGATGCAACTGAGGCGGTGTCAACTAATTCAGCACCAAAACCAACCATACAAGCTACTAGTTCTGAGGGTGCAGCCTTGAGGTATCCTGTGCGCACTGCTTCGGTTGATCCTTCCAATTCAAAAGATATTACCACTTCGTCTATGGAATCAAAACCTATTGCATCGGCACCTGAGATTACTGATGCTGATGCTGTTTTTGTAGCATCACAACCTCTTCCTTTGAGTCCTAGGTTGTCAGGGAAACTTTCTGGTTTAAGAAGCCCGACAGATAGCAGTGATCATGGTGGTGACCAACAAGTTAATGAGTACTTAGTTGACAGGCAGATGAATACTGCTCGTTCAAACTTGTCTGATATGCCTGCACTCGATGATGATGCAAGGAATGATGAGCAGAAAATTGCACAAGACGAAGTATCTAGTGTGCTCAATCCTCCTATTATGTTCAAACATCCAACCCATTTAATAACTCCCTCTGAGATTTTAATGGCTGCTTCATCCTCTGAGAATACAAATCCAATTGACAGCAACACTGAGGGGGATGCAAAAATCCAAGATGTTCTTGTTAACAATGATGTGGGTAATCCTGAGGTGGAAGTTAAGGTTGTGGGTGAAACCAGATCTACTCAAATCGATGATTTTGGGTCTCAACGAGAACTCCAAAATGTTGTTTCAGAAAATAAGGAGAAGATCTTTTGCTCCCAGGCATCAGATCTTGGAATTGAGATGGCCCGGGATTGCTGTGCAATATCATCAGAATCTTATATTACAGATGAAGCTCGGCAAGGTGATGGTGCCAGCATGTCAGAACCACTAGCCCAGCCTCATTCTGGTGAGGAAGACCAGGATCTCTCTACAAAAGATGTATCTGGGTCAGCTAACGCCACAACCACATCACAAACACCAAATGCAAAGTCGAGAAAACAGAAATGGAAAAATATGCAAGCATCAGGTCCATCTTCCCCATCACCAGGTGTGCTCAATGCAGTAGAGTCCTCCAATGAAGCAGGTGGGAGTTCAAGTCCACCCTCCGGAGAAGCTGCATTTCCTCAAATTATGGCCATGCAGGATATGATGAATCAGCTAATGACTATGCAAAAAGAATTGCAAAAGCAGATGACAATGATGGTTACAGTCCCAATTACAAAGGAAGGCAAACGATTAGAGGTGGCTCTCGGTCGGAGTATGGAGAAGGCTGTAAAAGCCAATAATGATGCTTTGTGGGCTCGGTTTCAAGAAGAGAATGCAAAGAATGAGAAATTATTGCGAGACCGTACTCAACAAGTAACTAGTTTGATCAATAACTTTGTGAACAAGGACTTTCCAGTCATGTTAGAGAAAACAGTAAAAAAAGAAATAGCTGCTGTTGGACCAGCTGTTGTTCGTGCAATCACTCCAGCTCTGGAGAAAACAATACCTTTGGCTATATCTGATTGCTTTCAGAGGGGAGTGGGAGATAAAGCTGTTAATCAATTGGAAAAATCAGTTAACTCAAAACTTGAAGCTACTGTATCCAGGCAGATCCAGGCGCAGTTTCAAACATCGGGTAAACAAGCTCTTCAGGATGCTCTCAAGTCTAGCATGGAAGCTTCAGTGGTACCTGCCTTTGAGAAGTCATGTAAAGCCATGTTTGAGCAAGTAGATGCCACATTCCAGAAGGGAATGGTTGAACATGCAACTGTGGCTCAGCAGCATTTTGAGTCTGCACATTCGCCGTTGGCCCATGCTTTAAGGGAAGCTATCAGCTCCGCGTCATCAGTGACTCAAACCCTAAGTGGGGAACTGGCTGATGGTCAACGTAAGTTAGTAGCTCTTGCAGCTGCACGGGGAAACTCAAGTGCTGTAAATCCCCTAGTCACTCAACTAACCAATGGACCTTTGGGTGGTCTCCATGAGAAGGTTGAGGTGCCTTTGGATCCAACAAAGGAGCTGTCAAGGTTGGTAACTGAACGCAAGTATGAGGAGGCTTTCACAGGTGCTCTACAAAGAAGTGATGTTGGCATTGTATCATGGTTATGTGCTCAGGTTGATCTTCATGGGATACTGTTGCTGCAACCTGTTCCTCTGAGCCAAGGAGTACTTCTGTCACTCCTGCAACAGCTGGCCTGTGATATCAACAATGATACACCCCGGAAAGTTGCATGGATGACAGACGTGGCAGCCGCCATAAACCCATCCAACCAAATGATTGCAATGCACGTGAGACCCATCTTTGAGCAAGTTTATCAGATACTGCACCACCAACATAGCTTGCCTACGATCTCCAGTGCCGAGCATGCTAGTCTTCGTCTTCTCATGCATGTCATTAACTCCATGATGATGGCCTGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000932 GO:0000956 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009892 GO:0009933 GO:0009965 GO:0009987 GO:0010014 GO:0010015 GO:0010016 GO:0010033 GO:0010071 GO:0010072 GO:0010154 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0022414 GO:0022622 GO:0023052 GO:0031086 GO:0031087 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0042221 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046700 GO:0046983 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048532 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071704 GO:0090304 GO:0090305 GO:0090421 GO:0097159 GO:0099402 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
Ge1_WD40

Protein Analysis

1398

Amino Acids

151.32

Weight (kDa)

5.68

Isoelectric Point (pI)

44.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ge1_WD40 PF16529 231 - 543 2.9e-18 WD40 region of Ge1, enhancer of mRNA-decapping protein
WD40_WDHD1_1st PF24817 249 - 368 1.8e-06 WDHD1 first WD40 domain
EDC4_C PF21289 1266 - 1369 1.5e-16 Enhancer of mRNA-decapping protein 4, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13290 AT3G13300 AT3G13300 AT3G13300
fragaria_vesca FvH4_3g41972 FvH4_3g41980 FvH4_3g41980 FvH4_3g41980 FvH4_3g41980 FvH4_3g42780 FvH4_3g42790 FvH4_3g42820 FvH4_3g42820 FvH4_3g42820 FvH4_3g42820 FvH4_4g01881 FvH4_7g10420
malus_domestica MD00G1013700.v1.1 MD03G1039800.v1.1 MD11G1041300.v1.1 MD11G1094900.v1.1
prunus_persica Prupe.6G032100_v2.0.a1
pyrus_communis pycom03g03070 pycom11g03500 pycom11g08470 pycom12g06730
rosa_chinensis RchiOBHm_Chr1g0328831 RchiOBHm_Chr4g0386471 RchiOBHm_Chr4g0386481 RchiOBHm_Chr4g0386551 RchiOBHm_Chr4g0386701 RchiOBHm_Chr4g0386801 RchiOBHm_Chr4g0386811 RchiOBHm_Chr4g0386821 RchiOBHm_Chr4g0386921 RchiOBHm_Chr4g0386931 RchiOBHm_Chr4g0386941 RchiOBHm_Chr4g0387211 RchiOBHm_Chr4g0389101 RchiOBHm_Chr4g0389111 RchiOBHm_Chr4g0389201 RchiOBHm_Chr4g0389211 RchiOBHm_Chr4g0389321 RchiOBHm_Chr4g0389331 RchiOBHm_Chr4g0389341 RchiOBHm_Chr4g0389411 RchiOBHm_Chr5g0075171
rosa_laevigata RLG00000009985 RLG00000009990 RLG00000009996 RLG00000009998 RLG00000010136 RLG00000010140 RLG00000036537
rosa_multiflora Rmu_co8461959.1_g000001 Rmu_sc0000013.1_g000017 Rmu_sc0000013.1_g000024 Rmu_sc0001015.1_g000001 Rmu_sc0001395.1_g000004 Rmu_sc0001942.1_g000022 Rmu_sc0002498.1_g000018 Rmu_sc0002498.1_g000020 Rmu_sc0002557.1_g000005 Rmu_sc0002604.1_g000010 Rmu_sc0002604.1_g000011 Rmu_sc0004750.1_g000003 Rmu_sc0006571.1_g000001 Rmu_sc0006571.1_g000014 Rmu_sc0006571.1_g000018 Rmu_sc0008050.1_g000016 Rmu_sc0008066.1_g000009 Rmu_sc0008380.1_g000006 Rmu_sc0008380.1_g000010 Rmu_sc0008380.1_g000012 Rmu_sc0010272.1_g000021 Rmu_sc0010693.1_g000010 Rmu_sc0010693.1_g000012 Rmu_sc0010693.1_g000014 Rmu_sc0010693.1_g000015 Rmu_sc0031790.1_g000003 Rmu_sc0040539.1_g000001 Rmu_ssc0000211.1_g000001 Rmu_ssc0000211.1_g000011 Rmu_ssc0000211.1_g000013
rosa_roxburghii Rroxscaffold_1G00006420 Rroxscaffold_1G00006430 Rroxscaffold_5G00334020 Rroxscaffold_5G00334050 Rroxscaffold_5G00335420
rosa_rugosa Rorug03G0307600 Rorug03G0308600 Rorug03G0322400 Rorug05G0437200 Rorug05G0437200 Rorug05G0437200
rosa_samantha Rh1BG075700 Rh4BG010000 Rh4BG010100 Rh4BG010200 Rh4BG020000 Rh4BG020400 Rh4BG172100 Rh4CG015300 Rh4CG016300 Rh5AG494100 Rh5AG494200 Rh5BG515500 Rh5CG539300 Rh5DG528200
rosa_wichuraiana Rw4G000930 Rw4G001980 Rw4G001990 Rw4G011950 Rw5G045850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1397
AarI CACCTGC 1 cut(s) 2858
AasI GACNNNNNNGTC 1 cut(s) 3185
Acc16I TGCGCA 1 cut(s) 1955
Acc36I ACCTGC 2 cut(s) 2858, 3469
Acc65I GGTACC 1 cut(s) 3457
AccB1I GGYRCC 7 cut(s) 229, 449, 547, 2029, 2654, 3457, 3766
AccBSI CCGCTC 1 cut(s) 534
AccI GTMKAC 1 cut(s) 1352
AccII CGCG 3 cut(s) 401, 749, 3611
AccIII TCCGGA 1 cut(s) 2891
AclWI GGATC 9 cut(s) 713, 726, 1136, 1149, 1964, 2715, 3376, 3770, 3783
AcoI YGGCCR 2 cut(s) 60, 2919
AcsI RAATTY 4 cut(s) 297, 933, 1676, 1825
AcuI CTGAAG 4 cut(s) 960, 1422, 3404, 3435
AcvI CACGTG 1 cut(s) 4060
AcyI GRCGYC 3 cut(s) 5, 230, 450
AdeI CACNNNGTG 1 cut(s) 2834
AfaI GTAC 8 cut(s) 257, 961, 1317, 1492, 2171, 3136, 3459, 3939
AhlI ACTAGT 2 cut(s) 1919, 3149
AjiI CACGTC 1 cut(s) 4015
AjnI CCWGG 7 cut(s) 120, 360, 2562, 2703, 2830, 3374, 3384
AjuI GAANNNNNNNTTGG 4 cut(s) 1817, 1849, 3924, 3956
Alw21I GWGCWC 8 cut(s) 478, 841, 1517, 1626, 2289, 2841, 3841, 3883
Alw26I GTCTC 5 cut(s) 1315, 2511, 3123, 3752, 4057
Alw44I GTGCAC 1 cut(s) 1622
AlwI GGATC 9 cut(s) 713, 726, 1136, 1149, 1964, 2715, 3376, 3770, 3783
AlwNI CAGNNNCTG 1 cut(s) 4093
Ama87I CYCGRG 3 cut(s) 665, 1527, 2592
Aor13HI TCCGGA 1 cut(s) 2891
ApaI GGGCCC 2 cut(s) 207, 1409
ApaLI GTGCAC 1 cut(s) 1622
ApoI RAATTY 4 cut(s) 297, 933, 1676, 1825
ArsI GACNNNNNNTTYG 4 cut(s) 2759, 2791, 3776, 3808
Asp700I GAANNNNTTC 1 cut(s) 1020
Asp718I GGTACC 1 cut(s) 3457
AspA2I CCTAGG 1 cut(s) 2090
AspLEI GCGC 4 cut(s) 232, 452, 1956, 3391
AvaI CYCGRG 3 cut(s) 665, 1527, 2592
AvaII GGWCC 5 cut(s) 944, 1180, 2813, 3230, 3734
AvrII CCTAGG 1 cut(s) 2090
AxyI CCTNAGG 2 cut(s) 160, 2448
BaeGI GKGCMC 4 cut(s) 207, 552, 1409, 1626
BaeI ACNNNNGTAYC 2 cut(s) 624, 657
BalI TGGCCA 2 cut(s) 62, 2921
BamHI GGATCC 3 cut(s) 718, 1141, 3775
BanI GGYRCC 7 cut(s) 229, 449, 547, 2029, 2654, 3457, 3766
BanII GRGCYC 5 cut(s) 207, 841, 1409, 1517, 3088
BauI CACGAG 1 cut(s) 1029
BbrPI CACGTG 1 cut(s) 4060
BbsI GAAGAC 4 cut(s) 1428, 2706, 4136, 4142
Bbv12I GWGCWC 8 cut(s) 478, 841, 1517, 1626, 2289, 2841, 3841, 3883
BbvCI CCTCAGC 3 cut(s) 270, 723, 1137
BceAI ACGGC 4 cut(s) 217, 243, 932, 3559
BcgI CGANNNNNNTGC 2 cut(s) 2219, 2253
BciT130I CCWGG 7 cut(s) 122, 362, 2564, 2705, 2832, 3376, 3386
BciVI GTATCC 4 cut(s) 625, 1956, 3382, 3894
BclI TGATCA 3 cut(s) 1381, 2140, 3156
BcoDI GTCTC 5 cut(s) 1315, 2511, 3123, 3752, 4057
BcuI ACTAGT 2 cut(s) 1919, 3149
BfoI RGCGCY 2 cut(s) 233, 453
BfuAI ACCTGC 2 cut(s) 2858, 3469
BfuI GTATCC 4 cut(s) 625, 1956, 3382, 3894
BglI GCCNNNNNGGC 1 cut(s) 401
BglII AGATCT 6 cut(s) 824, 1281, 1428, 2480, 2550, 2572
BlnI CCTAGG 1 cut(s) 2090
BlpI GCTNAGC 1 cut(s) 3546
BmcAI AGTACT 3 cut(s) 1317, 2171, 3939
Bme18I GGWCC 5 cut(s) 944, 1180, 2813, 3230, 3734
BmeT110I CYCGRG 3 cut(s) 665, 1527, 2592
BmgBI CACGTC 1 cut(s) 4015
BmrI ACTGGG 2 cut(s) 1461, 1719
BmuI ACTGGG 2 cut(s) 1461, 1719
BpiI GAAGAC 4 cut(s) 1428, 2706, 4136, 4142
BplI GAGNNNNNCTC 2 cut(s) 1504, 1536
BpmI CTGGAG 4 cut(s) 469, 3240, 3284, 4108
Bpu10I CCTNAGC 4 cut(s) 270, 723, 1137, 3882
Bpu1102I GCTNAGC 1 cut(s) 3546
BpuEI CTTGAG 4 cut(s) 294, 1960, 3416, 3679
Bsa29I ATCGAT 1 cut(s) 2493
BsaAI YACGTR 1 cut(s) 4060
BsaHI GRCGYC 3 cut(s) 5, 230, 450
BsaI GGTCTC 5 cut(s) 1315, 2511, 3123, 3752, 4057
BsaWI WCCGGW 1 cut(s) 2891
BsaXI ACNNNNNCTCC 4 cut(s) 2502, 2532, 2866, 2896
Bse1I ACTGG 9 cut(s) 253, 486, 518, 1467, 1714, 1851, 3185, 3648, 4125
Bse21I CCTNAGG 2 cut(s) 160, 2448
Bse3DI GCAATG 2 cut(s) 381, 4059
BseAI TCCGGA 1 cut(s) 2891
BseBI CCWGG 7 cut(s) 122, 362, 2564, 2705, 2832, 3376, 3386
BseCI ATCGAT 1 cut(s) 2493
BseMI GCAATG 2 cut(s) 381, 4059
BseNI ACTGG 9 cut(s) 253, 486, 518, 1467, 1714, 1851, 3185, 3648, 4125
BseRI GAGGAG 4 cut(s) 148, 183, 397, 3836
BseSI GKGCMC 4 cut(s) 207, 552, 1409, 1626
BseYI CCCAGC 2 cut(s) 108, 2679
BsgI GTGCAG 5 cut(s) 1953, 2208, 3549, 3666, 4079
Bsh1236I CGCG 3 cut(s) 401, 749, 3611
Bsh1285I CGRYCG 2 cut(s) 338, 3040
BshNI GGYRCC 7 cut(s) 229, 449, 547, 2029, 2654, 3457, 3766
BshVI ATCGAT 1 cut(s) 2493
BsiEI CGRYCG 2 cut(s) 338, 3040
BsiHKAI GWGCWC 8 cut(s) 478, 841, 1517, 1626, 2289, 2841, 3841, 3883
BsiHKCI CYCGRG 3 cut(s) 665, 1527, 2592
BslFI GGGAC 4 cut(s) 506, 1112, 1726, 2981
BsmAI GTCTC 5 cut(s) 1315, 2511, 3123, 3752, 4057
BsmFI GGGAC 4 cut(s) 506, 1112, 1726, 2981
BsmI GAATGC 4 cut(s) 594, 1541, 1612, 3109
Bso31I GGTCTC 5 cut(s) 1315, 2511, 3123, 3752, 4057
BsoBI CYCGRG 3 cut(s) 665, 1527, 2592
Bsp120I GGGCCC 2 cut(s) 203, 1405
Bsp13I TCCGGA 1 cut(s) 2891
Bsp1720I GCTNAGC 1 cut(s) 3546
BspDI ATCGAT 1 cut(s) 2493
BspEI TCCGGA 1 cut(s) 2891
BspFNI CGCG 3 cut(s) 401, 749, 3611
BspMI ACCTGC 2 cut(s) 2858, 3469
BspPI GGATC 9 cut(s) 713, 726, 1136, 1149, 1964, 2715, 3376, 3770, 3783
BspQI GCTCTTC 1 cut(s) 3423
BspT107I GGYRCC 7 cut(s) 229, 449, 547, 2029, 2654, 3457, 3766
BspTNI GGTCTC 5 cut(s) 1315, 2511, 3123, 3752, 4057
BsrBI CCGCTC 1 cut(s) 534
BsrDI GCAATG 2 cut(s) 381, 4059
BsrI ACTGG 9 cut(s) 253, 486, 518, 1467, 1714, 1851, 3185, 3648, 4125
BssNI GRCGYC 3 cut(s) 5, 230, 450
BssSI CACGAG 1 cut(s) 1029
BssT1I CCWWGG 2 cut(s) 2090, 3931
Bst2BI CACGAG 1 cut(s) 1029
Bst2UI CCWGG 7 cut(s) 122, 362, 2564, 2705, 2832, 3376, 3386
Bst6I CTCTTC 4 cut(s) 561, 2082, 3093, 3423
BstACI GRCGYC 3 cut(s) 5, 230, 450
BstBAI YACGTR 1 cut(s) 4060
BstDSI CCRYGG 1 cut(s) 399
BstEII GGTNACC 1 cut(s) 2150
BstFNI CGCG 3 cut(s) 401, 749, 3611
BstH2I RGCGCY 2 cut(s) 233, 453
BstHHI GCGC 4 cut(s) 232, 452, 1956, 3391
BstMAI GTCTC 5 cut(s) 1315, 2511, 3123, 3752, 4057
BstMCI CGRYCG 2 cut(s) 338, 3040
BstNI CCWGG 7 cut(s) 122, 362, 2564, 2705, 2832, 3376, 3386
BstNSI RCATGY 4 cut(s) 2665, 3536, 4139, 4163
BstPI GGTNACC 1 cut(s) 2150
BstSLI GKGCMC 4 cut(s) 207, 552, 1409, 1626
BstUI CGCG 3 cut(s) 401, 749, 3611
BstV2I GAAGAC 4 cut(s) 1428, 2706, 4136, 4142
BstXI CCANNNNNNTGG 4 cut(s) 1190, 1249, 1660, 4180
Bsu15I ATCGAT 1 cut(s) 2493
Bsu36I CCTNAGG 2 cut(s) 160, 2448
BsuI GTATCC 4 cut(s) 625, 1956, 3382, 3894
BsuTUI ATCGAT 1 cut(s) 2493
BtgI CCRYGG 1 cut(s) 399
BtrI CACGTC 1 cut(s) 4015
BtsI GCAGTG 3 cut(s) 813, 1956, 2143
BveI ACCTGC 2 cut(s) 2858, 3469
CaiI CAGNNNCTG 1 cut(s) 4093
CfoI GCGC 4 cut(s) 232, 452, 1956, 3391
Cfr42I CCGCGG 1 cut(s) 402
Cfr9I CCCGGG 2 cut(s) 665, 2592
ClaI ATCGAT 1 cut(s) 2493
CseI GACGC 1 cut(s) 3600
CsiI ACCWGGT 1 cut(s) 2830
Csp6I GTAC 8 cut(s) 256, 960, 1316, 1491, 2170, 3135, 3458, 3938
CviQI GTAC 8 cut(s) 256, 960, 1316, 1491, 2170, 3135, 3458, 3938
DinI GGCGCC 2 cut(s) 231, 451
DraI TTTAAA 1 cut(s) 73
DraIII CACNNNGTG 1 cut(s) 2834
DrdI GACNNNNNNGTC 1 cut(s) 3185
DseDI GACNNNNNNGTC 1 cut(s) 3185
EaeI YGGCCR 2 cut(s) 60, 2919
Eam1104I CTCTTC 4 cut(s) 561, 2082, 3093, 3423
EarI CTCTTC 4 cut(s) 561, 2082, 3093, 3423
EciI GGCGGA 2 cut(s) 15, 382
Ecl136II GAGCTC 2 cut(s) 839, 1515
Eco130I CCWWGG 2 cut(s) 2090, 3931
Eco24I GRGCYC 5 cut(s) 207, 841, 1409, 1517, 3088
Eco31I GGTCTC 5 cut(s) 1315, 2511, 3123, 3752, 4057
Eco32I GATATC 1 cut(s) 3973
Eco47I GGWCC 5 cut(s) 944, 1180, 2813, 3230, 3734
Eco53kI GAGCTC 2 cut(s) 839, 1515
Eco57I CTGAAG 4 cut(s) 960, 1422, 3404, 3435
Eco72I CACGTG 1 cut(s) 4060
Eco81I CCTNAGG 2 cut(s) 160, 2448
Eco88I CYCGRG 3 cut(s) 665, 1527, 2592
Eco91I GGTNACC 1 cut(s) 2150
EcoICRI GAGCTC 2 cut(s) 839, 1515
EcoO109I RGGNCCY 2 cut(s) 124, 204
EcoO65I GGTNACC 1 cut(s) 2150
EcoRI GAATTC 1 cut(s) 1825
EcoRII CCWGG 7 cut(s) 120, 360, 2562, 2703, 2830, 3374, 3384
EcoRV GATATC 1 cut(s) 3973
EcoT14I CCWWGG 2 cut(s) 2090, 3931
EcoT22I ATGCAT 2 cut(s) 1543, 4161
EcoT38I GRGCYC 5 cut(s) 207, 841, 1409, 1517, 3088
EgeI GGCGCC 2 cut(s) 231, 451
EheI GGCGCC 2 cut(s) 231, 451
ErhI CCWWGG 2 cut(s) 2090, 3931
FalI AAGNNNNNCTT 8 cut(s) 903, 935, 1563, 1595, 2406, 2438, 3924, 3956
FaqI GGGAC 4 cut(s) 506, 1112, 1726, 2981
FauI CCCGC 3 cut(s) 363, 527, 532
FbaI TGATCA 3 cut(s) 1381, 2140, 3156
FblI GTMKAC 1 cut(s) 1352
FriOI GRGCYC 5 cut(s) 207, 841, 1409, 1517, 3088
FspAI RTGCGCAY 1 cut(s) 1955
FspI TGCGCA 1 cut(s) 1955
GlaI GCGC 4 cut(s) 231, 451, 1955, 3390
GsaI CCCAGC 2 cut(s) 112, 2683
GsuI CTGGAG 4 cut(s) 469, 3240, 3284, 4108
HaeII RGCGCY 2 cut(s) 233, 453
HgaI GACGC 1 cut(s) 3600
HhaI GCGC 4 cut(s) 232, 452, 1956, 3391
Hin1I GRCGYC 3 cut(s) 5, 230, 450
Hin6I GCGC 4 cut(s) 230, 450, 1954, 3389
HinP1I GCGC 4 cut(s) 230, 450, 1954, 3389
HincII GTYRAC 5 cut(s) 1884, 2179, 2428, 3349, 3656
HindII GTYRAC 5 cut(s) 1884, 2179, 2428, 3349, 3656
HindIII AAGCTT 2 cut(s) 1166, 3447
HpaI GTTAAC 2 cut(s) 2428, 3349
HpyCH4IV ACGT 3 cut(s) 3658, 4014, 4059
HpySE526I ACGT 3 cut(s) 3658, 4014, 4059
Hsp92I GRCGYC 3 cut(s) 5, 230, 450
HspAI GCGC 4 cut(s) 230, 450, 1954, 3389
KasI GGCGCC 2 cut(s) 229, 449
Kpn2I TCCGGA 1 cut(s) 2891
KpnI GGTACC 1 cut(s) 3461
Ksp22I TGATCA 3 cut(s) 1381, 2140, 3156
KspAI GTTAAC 2 cut(s) 2428, 3349
KspI CCGCGG 1 cut(s) 402
LguI GCTCTTC 1 cut(s) 3423
LmnI GCTCC 7 cut(s) 370, 481, 531, 1372, 2564, 3611, 3787
MabI ACCWGGT 1 cut(s) 2830
MaeII ACGT 3 cut(s) 3658, 4014, 4059
MbiI CCGCTC 1 cut(s) 534
MfeI CAATTG 2 cut(s) 2379, 3332
MlsI TGGCCA 2 cut(s) 62, 2921
MluNI TGGCCA 2 cut(s) 62, 2921
Mly113I GGCGCC 2 cut(s) 230, 450
MlyI GAGTC 6 cut(s) 1487, 1539, 2095, 2861, 3569, 3616
MmeI TCCRAC 6 cut(s) 435, 2339, 3020, 3208, 3803, 4062
Mox20I TGGCCA 2 cut(s) 62, 2921
Mph1103I ATGCAT 2 cut(s) 1543, 4161
MroI TCCGGA 1 cut(s) 2891
MroXI GAANNNNTTC 1 cut(s) 1020
MscI TGGCCA 2 cut(s) 62, 2921
MslI CAYNNNNRTG 7 cut(s) 860, 1486, 1658, 2148, 2691, 3874, 4178
Msp20I TGGCCA 2 cut(s) 62, 2921
MspA1I CMGCKG 8 cut(s) 322, 334, 401, 685, 727, 3236, 3680, 3961
MunI CAATTG 2 cut(s) 2379, 3332
Mva1269I GAATGC 4 cut(s) 594, 1541, 1612, 3109
MvaI CCWGG 7 cut(s) 122, 362, 2564, 2705, 2832, 3376, 3386
MvnI CGCG 3 cut(s) 401, 749, 3611
NarI GGCGCC 2 cut(s) 230, 450
NmeAIII GCCGAG 5 cut(s) 618, 901, 1225, 2620, 4156
NmuCI GTSAC 9 cut(s) 853, 1210, 1299, 1360, 1566, 2150, 3619, 3715, 3945
NsbI TGCGCA 1 cut(s) 1955
NsiI ATGCAT 2 cut(s) 1543, 4161
NspI RCATGY 4 cut(s) 2665, 3536, 4139, 4163
PaeI GCATGC 1 cut(s) 4139
PaeR7I CTCGAG 1 cut(s) 1527
PaqCI CACCTGC 1 cut(s) 2858
PciSI GCTCTTC 1 cut(s) 3423
PctI GAATGC 4 cut(s) 594, 1541, 1612, 3109
PdmI GAANNNNTTC 1 cut(s) 1020
PfeI GAWTC 6 cut(s) 456, 568, 1847, 2009, 2618, 2938
PleI GAGTC 6 cut(s) 1486, 1538, 2094, 2860, 3568, 3616
PluTI GGCGCC 2 cut(s) 233, 453
PmaCI CACGTG 1 cut(s) 4060
PmlI CACGTG 1 cut(s) 4060
PpsI GAGTC 6 cut(s) 1486, 1538, 2094, 2860, 3568, 3616
Ppu21I YACGTR 1 cut(s) 4060
PsiI TTATAA 1 cut(s) 1397
Psp124BI GAGCTC 2 cut(s) 841, 1517
Psp6I CCWGG 7 cut(s) 120, 360, 2562, 2703, 2830, 3374, 3384
PspCI CACGTG 1 cut(s) 4060
PspEI GGTNACC 1 cut(s) 2150
PspFI CCCAGC 2 cut(s) 108, 2679
PspGI CCWGG 7 cut(s) 120, 360, 2562, 2703, 2830, 3374, 3384
PspOMI GGGCCC 2 cut(s) 203, 1405
PspXI VCTCGAGB 1 cut(s) 1527
PstNI CAGNNNCTG 1 cut(s) 4093
PvuII CAGCTG 6 cut(s) 322, 685, 727, 3236, 3680, 3961
RsaI GTAC 8 cut(s) 257, 961, 1317, 1492, 2171, 3136, 3459, 3939
RsaNI GTAC 8 cut(s) 256, 960, 1316, 1491, 2170, 3135, 3458, 3938
RseI CAYNNNNRTG 7 cut(s) 860, 1486, 1658, 2148, 2691, 3874, 4178
SacI GAGCTC 2 cut(s) 841, 1517
SacII CCGCGG 1 cut(s) 402
SapI GCTCTTC 1 cut(s) 3423
ScaI AGTACT 3 cut(s) 1317, 2171, 3939
SchI GAGTC 6 cut(s) 1487, 1539, 2095, 2861, 3569, 3616
SexAI ACCWGGT 1 cut(s) 2830
SfoI GGCGCC 2 cut(s) 231, 451
Sfr274I CTCGAG 1 cut(s) 1527
Sfr303I CCGCGG 1 cut(s) 402
SgrBI CCGCGG 1 cut(s) 402
SinI GGWCC 5 cut(s) 944, 1180, 2813, 3230, 3734
SlaI CTCGAG 1 cut(s) 1527
SmaI CCCGGG 2 cut(s) 667, 2594
SmiMI CAYNNNNRTG 7 cut(s) 860, 1486, 1658, 2148, 2691, 3874, 4178
SmlI CTYRAG 5 cut(s) 309, 1527, 1939, 3431, 3694
SmoI CTYRAG 5 cut(s) 309, 1527, 1939, 3431, 3694
SpeI ACTAGT 2 cut(s) 1919, 3149
SphI GCATGC 1 cut(s) 4139
SspDI GGCGCC 2 cut(s) 229, 449
SspI AATATT 1 cut(s) 793
SstI GAGCTC 2 cut(s) 841, 1517
StyI CCWWGG 2 cut(s) 2090, 3931
TaiI ACGT 3 cut(s) 3661, 4017, 4062
TaqI TCGA 9 cut(s) 54, 542, 586, 835, 1528, 1533, 2229, 2493, 2777
TaqII GACCGA 1 cut(s) 3026
TatI WGTACW 3 cut(s) 1315, 2169, 3937
TfiI GAWTC 6 cut(s) 456, 568, 1847, 2009, 2618, 2938
TseFI GTSAC 9 cut(s) 853, 1210, 1299, 1360, 1566, 2150, 3619, 3715, 3945
Tsp45I GTSAC 9 cut(s) 853, 1210, 1299, 1360, 1566, 2150, 3619, 3715, 3945
TspDTI ATGAA 8 cut(s) 966, 1013, 2204, 2346, 2649, 2877, 2951, 3884
TspMI CCCGGG 2 cut(s) 665, 2592
VneI GTGCAC 1 cut(s) 1622
VpaK11BI GGWCC 5 cut(s) 944, 1180, 2813, 3230, 3734
XapI RAATTY 4 cut(s) 297, 933, 1676, 1825
XbaI TCTAGA 1 cut(s) 490
XceI RCATGY 4 cut(s) 2665, 3536, 4139, 4163
XcmI CCANNNNNNNNNTGG 7 cut(s) 358, 493, 559, 1035, 2002, 2687, 3736
XhoI CTCGAG 1 cut(s) 1527
XmaI CCCGGG 2 cut(s) 665, 2592
XmaJI CCTAGG 1 cut(s) 2090
XmiI GTMKAC 1 cut(s) 1352
XmnI GAANNNNTTC 1 cut(s) 1020
ZrmI AGTACT 3 cut(s) 1317, 2171, 3939
Zsp2I ATGCAT 2 cut(s) 1543, 4161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.