MD03G1039800.v1.1

Enhancer of mRNA-decapping protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000324
Physical Location & Seq
Forward (+)
3153002 .. 3161046
8045 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1039800.v1.1.491

Sequence Viewer

Length: 4242 bp
ATGGCTTCCACCGGGCCAAATCCGCCGCCACAGCAGCAACCGCAGCCCTTCGACATGCAGAAGTTCTTTAAACCGACAAACCCAGCTGCTCTCACCGCCCAAACGTCAACCCCCGCCAATTCAAACCAAAACCCTAACCCTAATTTGAGTTCCTCCTCGTTCCCAGTCCCGCCCGCTTCTTTCCCTCCTCCGACGGGCCCCTACTCGTACCCGCCCCAAACGGCACCGTTTCACCACCATCAGTTTCAATACCTCCCCCAACCGCAGCAGCCCCACCCCCAAATCCAATACTCTCCGCAGGACCACCACTTGCTCCAGCAGAGGTCTCTCTCGTTCCCCACCCCGCCCCTCCAACCCCCTGGTAATTACAACATTGCCACTCCCACTTCCAACCCCAGCGGCACCGGCAGTGGTAATTCCAATTCGAGCCCCAATTCCGGTGCTCGGATCATGGCGCTTCTTGGTGGTCCGTCAGGAAATCTAGAGCCTGAGTTGCCGACTCCTCCAGGTGTCTCAATGCCGCCCACTCTCCCAATGGGGATTCAACCCACTGGCCCGATTCGAATGCCCAGCAATAAGCTACCGAAAGGCCGGCATTTGGTTGGCAATAACGTGGTTTATGATGTGGACGTTAGGTTGCAGGGTGAGGTTCAGCCACAGCTCGAAGTTACACCCATAACCAAGTATGGTTCGGATCCTCAGCTTGTGCTTGGCCGCCAAATTGCGGTTAATAAGTCGTATATATGCTATGGATTGAAGCAGGGCAACATTCGGGTGTTGAATATACACACTGCATTGAGATCTTTGTTTCGAGCTCATACACAGAGGGTCACAGACATGGCTTTCTTTGCTGAGGATGTTCACCTTTTGGCTAGTGTGAGTGTTGAAGGAAGACTCTTTGTATGGAAAATTTCGGAAGGTCCAGATGAAGAAGGTACGCCACAAATCACTGGAAAGGTTGTGATTGCTATTCAAATAGTTGGAGAGGGGGAAGCTGTTCATCCACGAGTTTGTTGGCACTGTCATAAACAAGAGGTTTTGGTGGTTGGGTTTGGAAAGCGTGTACTAAGAATTGATACTACTAAAGTTGTGAAGGGTGAACCCCCTTCTGCTGATGAGCCTCTCAAATGTCCTGTTGAAAAGCTGATTGATGGAGTCCAGTTTGTCGGTAAACATGATGGAGAAGTGACCGACTTGTCAATGTGCCAATGGATGACCACCCGTCTGGTTTCTGCTTCTATGGATGGAACAATAAAGATCTGGGAAGATCGCAAAGCTCAACCTCTTTTAGTATTGAGACCCTACGATGGGCAACCTGTTTATTCAGCTACATTTGTGACAGCTCCCCACAGGCCAGATCATATTATACTTATCACAGTGGGGCCACTAAATCGGGAAGTGAAGATCTGGTCCTCGGCGAGTGAAGAAGGCTGGCTGCTTCCCAGTGATGCTGAATCATGGAAATGTACCCAGACATTAGAGTTGAAGAGTTCTTCTGAACCCCGAGTCGAGGAGGCATTCTTTAATCAAGTCATAGCATTGTCACAAGCTGGCCTTCTCTTACTTGCAAATGCTAAGAAGAATGCTATATATGCTGTACACTTAGAATTTGGTCCTGATCCAGCAGCAACGCGCATGGATTACATTGCAGAATTTACCGTTACCATGCCTATTTTAAGTTTTACTGGGACAAGTATATCTCCTCATGGTGAACAGATTGTTCAGGTTTATTGTGTACAGACTCTGGCCATTCAGCAGTATGCTTTGGAATTATCAAAGTGCTTGCCTCCACCACTGGATAATGTTGGATTAGAAAAGTCAGATTCCAATATCTCACGCGATGCAATTGGGGCTGAAGTATATGCTTTGGATCTATCTGGAAGTAAACCCACAGAGGTGTTGCTAGCCAATTCATCACGTAGACCAGCCGTACATGAAAGCAGTTCTGAGGGTGCAGCTGCCATGAGGTATCCTGTAAGCTCTAATTCTGTTGAGGCAATCACTTCAAAAGATATTACCACTTCAAGTACAGAATCAAGACCTGTTTCATTGGCATCGGCATCTAGTGATTCTGCTGTTGTATTTGTTGCATCACCATCTATTCCCTTGAGTCCTAGGTTGTCCAGGAAACTTTCTGGTATAAGAAGTCCAGCAGATAGATCTGAGCCGGGTCGCACACTTAATGACCATGTTGGAGACCAACAAGTCAATGATTACTCTATTGACAGGCAAATGATCACTCCTCGTTCAAACGTGTCAGATGTGCCTGCAGTGGATGATGACTCAAGGAATGATGAGCCTAAAGTTGGACAAGATGATTTATCTAGTGTGCTCAATCCTCCTATCATGTTCAAACATCCAACCCATCTAATAACTCCCTCTGAGATTTTAATGGCTGCTTCATCTTCTGAAGGAGCTAATACTGTTGACTGCAAGAGTGAGGGGGAGGGAAATATCCCAGATGTTCTTGTTAACAGTGATGTAGGTAATTCTGAGGTGGAGGTTAAAGTAGTGGGTGAAACTAGATCTACTCAAATTGATGAGTTTGTCTGTCAAGGAGAACCACAAAATGCTGTCTCAGAAAATAAGGAAAAATACTTTTGCTCCCAGGCATCAGATCTTGGAATTGAAATGGCCCGAGAATGCTGTGTGATACCAGCAGAAACTTATGTTACGGATGAAGCTCGGCAAGTTGATGGTGGCAGTGTGACAGAACCACCAGGCCAATCCCATGCTGGAGACGAAGACCAGGACTCTGCAAAAGATGTTTCTGGGTCATCTACCTCCCCAACAGTTTTGCAATCGCAGACATCAAATACAAAAGTGAAAAAACAGAAGTGGAAAAATTCTCAAGCATCAGGTTCATCATTACCATCAGCAAGTATGACCAATTCCATAGATTCCACCAATGAACATGGTGGGAGTTCAAGCCTACCCTCTGCAGAAGCTGCATACCTGCAAATTATGGCCATGCAGGATACGGTTAATCAGCTACTGACCATGCAGAAAGAAATGCAAAAGCAGATAACAATGATGGTCGCAGCCCCCGTTACAAAGGAAGGGAGGAGATTGGAAGCTGCTCTTGGTAAAAGTATGGAGAAGGCTTTAAAGGCCAATAATGATGCTCTATGGGCTCGTTTTCAAGAAGAGAATGCAAAGAATGAGAAGTTATTGCGAGACCGTAATCAGCAAATAACCAGTTTGATAAATAACTTAATGAACAAGGACTTTCCAGTCATGTTGGAGAAAGTGGTGAAGAAGGAATCATCTGTGATTGGACCAGCTGTTGTTCGTGCTATAACTCCAGCTATTGAGAAGGCAATACCTTTGGCTATTTCTGATTCCTTCCAGAGAGGAGTGGGTGACAAGGCTGTTAACCAATTGGAGAAATCTGTTAACTCAAAACTTGAAGCTACTGTTTCCAGGCAAATTCAGGCGCAGTTTCAAACTTCTGGCAAACAAGCTCTACAGGATGCTCTTAAGTCTAGTATGGAAGCTTCAGTGGTACCTGCCTTTGAGAAGTCATGTAAGGCCATGTTTGAGCAAGTAGATGCTACGTTCCAGAAAGGAATGGTTGAACACACAACTGTGGCCCAGCAGCATTTTGACTCCACACATTCGCCATTGGCACTGGCTCTAAGGGAAGCTATGAATTCTGCATCATCGGTGACTCAAACCTTAAGCGGAGAAGTGGCTGAGGGTCAACGTAAGCTCATAGCTCTTGCAGCTGCACGAGGAAACTCAAGCGCAGTAAATCCTCTGGTCACACAACTCACTAATGGACCTTTGGGTGGTCTCCATGAGAAGGTTGAGGTACCTTTGGATCCAAAGAATGAGCTATCAAGGCTGGTATCTGAACGCAAGTACGAGGAGGCTTTCATGGCAGCTCTGCAAAGAAGTGATGTTTCCATCGTATCGTGGTTATGCCATCAGGTTGATCTACGGGGGATATTGCTGTTGAATCCTGTTCCTCTGAGCCAAGGTGTGCTTCTTTCTCTTTTGCAACAGCTGGCCTGTGATATCAGCAATGATACACCCAGAAAAGTTGCGTGGATGACGGATGTGGCGGGCGCCACAAACCCGGCTAACCAAATGATTGCGATGCACGGTCGGCCCATATTTGAGCAAGTTTATAGCATACTGCACCATCAACATGCCTTGCCTGCGATCAACAGTGCCGAACACACTAGCATCCGACTTCTCATGCATGTCATCAACTCCATTATGATGGCCTGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000932 GO:0000956 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009892 GO:0009933 GO:0009965 GO:0009987 GO:0010014 GO:0010015 GO:0010016 GO:0010033 GO:0010071 GO:0010072 GO:0010154 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0022414 GO:0022622 GO:0023052 GO:0031086 GO:0031087 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0042221 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046700 GO:0046983 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048532 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071704 GO:0090304 GO:0090305 GO:0090421 GO:0097159 GO:0099402 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
Ge1_WD40

Protein Analysis

1414

Amino Acids

153.11

Weight (kDa)

5.76

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ge1_WD40 PF16529 223 - 535 4.5e-18 WD40 region of Ge1, enhancer of mRNA-decapping protein
WD40_WDHD1_1st PF24817 241 - 358 3.2e-06 WDHD1 first WD40 domain
EDC4_C PF21289 1282 - 1388 2.2e-15 Enhancer of mRNA-decapping protein 4, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13290 AT3G13300 AT3G13300 AT3G13300
fragaria_vesca FvH4_3g41972 FvH4_3g41980 FvH4_3g41980 FvH4_3g41980 FvH4_3g41980 FvH4_3g42780 FvH4_3g42790 FvH4_3g42820 FvH4_3g42820 FvH4_3g42820 FvH4_3g42820 FvH4_4g01881 FvH4_7g10420
malus_domestica MD00G1013700.v1.1 MD03G1039800.v1.1 MD11G1041300.v1.1 MD11G1094900.v1.1
prunus_persica Prupe.6G032100_v2.0.a1
pyrus_communis pycom03g03070 pycom11g03500 pycom11g08470 pycom12g06730
rosa_chinensis RchiOBHm_Chr1g0328831 RchiOBHm_Chr4g0386471 RchiOBHm_Chr4g0386481 RchiOBHm_Chr4g0386551 RchiOBHm_Chr4g0386701 RchiOBHm_Chr4g0386801 RchiOBHm_Chr4g0386811 RchiOBHm_Chr4g0386821 RchiOBHm_Chr4g0386921 RchiOBHm_Chr4g0386931 RchiOBHm_Chr4g0386941 RchiOBHm_Chr4g0387211 RchiOBHm_Chr4g0389101 RchiOBHm_Chr4g0389111 RchiOBHm_Chr4g0389201 RchiOBHm_Chr4g0389211 RchiOBHm_Chr4g0389321 RchiOBHm_Chr4g0389331 RchiOBHm_Chr4g0389341 RchiOBHm_Chr4g0389411 RchiOBHm_Chr5g0075171
rosa_laevigata RLG00000009985 RLG00000009990 RLG00000009996 RLG00000009998 RLG00000010136 RLG00000010140 RLG00000036537
rosa_multiflora Rmu_co8461959.1_g000001 Rmu_sc0000013.1_g000017 Rmu_sc0000013.1_g000024 Rmu_sc0001015.1_g000001 Rmu_sc0001395.1_g000004 Rmu_sc0001942.1_g000022 Rmu_sc0002498.1_g000018 Rmu_sc0002498.1_g000020 Rmu_sc0002557.1_g000005 Rmu_sc0002604.1_g000010 Rmu_sc0002604.1_g000011 Rmu_sc0004750.1_g000003 Rmu_sc0006571.1_g000001 Rmu_sc0006571.1_g000014 Rmu_sc0006571.1_g000018 Rmu_sc0008050.1_g000016 Rmu_sc0008066.1_g000009 Rmu_sc0008380.1_g000006 Rmu_sc0008380.1_g000010 Rmu_sc0008380.1_g000012 Rmu_sc0010272.1_g000021 Rmu_sc0010693.1_g000010 Rmu_sc0010693.1_g000012 Rmu_sc0010693.1_g000014 Rmu_sc0010693.1_g000015 Rmu_sc0031790.1_g000003 Rmu_sc0040539.1_g000001 Rmu_ssc0000211.1_g000001 Rmu_ssc0000211.1_g000011 Rmu_ssc0000211.1_g000013
rosa_roxburghii Rroxscaffold_1G00006420 Rroxscaffold_1G00006430 Rroxscaffold_5G00334020 Rroxscaffold_5G00334050 Rroxscaffold_5G00335420
rosa_rugosa Rorug03G0307600 Rorug03G0308600 Rorug03G0322400 Rorug05G0437200 Rorug05G0437200 Rorug05G0437200
rosa_samantha Rh1BG075700 Rh4BG010000 Rh4BG010100 Rh4BG010200 Rh4BG020000 Rh4BG020400 Rh4BG172100 Rh4CG015300 Rh4CG016300 Rh5AG494100 Rh5AG494200 Rh5BG515500 Rh5CG539300 Rh5DG528200
rosa_wichuraiana Rw4G000930 Rw4G001980 Rw4G001990 Rw4G011950 Rw5G045850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 1195, 2204, 3233
Acc36I ACCTGC 2 cut(s) 2964, 3517
Acc65I GGTACC 2 cut(s) 3505, 3814
AccB1I GGYRCC 5 cut(s) 223, 401, 3505, 3814, 4070
AccI GTMKAC 1 cut(s) 1921
AccII CGCG 2 cut(s) 1633, 1839
AclWI GGATC 7 cut(s) 455, 689, 702, 1613, 1878, 3818, 3831
AcoI YGGCCR 3 cut(s) 712, 1746, 2967
AcsI RAATTY 6 cut(s) 909, 1607, 1652, 2845, 3429, 3652
AcuI CTGAAG 3 cut(s) 1875, 2430, 3483
AcyI GRCGYC 1 cut(s) 4071
AflII CTTAAG 2 cut(s) 3479, 3679
AflIII ACRYGT 1 cut(s) 2253
AhdI GACNNNNNGTC 1 cut(s) 1221
AjnI CCWGG 7 cut(s) 358, 505, 2123, 2607, 2719, 2748, 3422
AjuI GAANNNNNNNTTGG 4 cut(s) 3066, 3098, 3972, 4004
AleI CACNNNNGTG 2 cut(s) 1895, 3587
Alw21I GWGCWC 3 cut(s) 445, 817, 2334
Alw26I GTCTC 8 cut(s) 330, 517, 1291, 2190, 2581, 2733, 3171, 3800
AlwI GGATC 7 cut(s) 455, 689, 702, 1613, 1878, 3818, 3831
AlwNI CAGNNNCTG 3 cut(s) 1744, 2948, 2995
Ama87I CYCGRG 2 cut(s) 1503, 2637
ApaI GGGCCC 1 cut(s) 200
ApoI RAATTY 6 cut(s) 909, 1607, 1652, 2845, 3429, 3652
Asp700I GAANNNNTTC 1 cut(s) 996
Asp718I GGTACC 2 cut(s) 3505, 3814
AspA2I CCTAGG 1 cut(s) 2114
AspLEI GCGC 5 cut(s) 457, 1635, 3439, 3749, 4073
AsuC2I CCSGG 3 cut(s) 13, 2169, 4082
AsuII TTCGAA 1 cut(s) 562
AsuNHI GCTAGC 1 cut(s) 1903
AvaI CYCGRG 2 cut(s) 1503, 2637
AvaII GGWCC 7 cut(s) 301, 467, 920, 1410, 1613, 3278, 3782
AvrII CCTAGG 1 cut(s) 2114
BaeGI GKGCMC 1 cut(s) 200
BalI TGGCCA 2 cut(s) 1748, 2969
BamHI GGATCC 2 cut(s) 694, 3823
BanI GGYRCC 5 cut(s) 223, 401, 3505, 3814, 4070
BanII GRGCYC 4 cut(s) 200, 431, 817, 3136
BarI GAAGNNNNNNTAC 2 cut(s) 3481, 3513
BauI CACGAG 2 cut(s) 1005, 3732
BbsI GAAGAC 2 cut(s) 898, 2751
Bbv12I GWGCWC 3 cut(s) 445, 817, 2334
BbvCI CCTCAGC 3 cut(s) 699, 852, 3696
BceAI ACGGC 2 cut(s) 237, 1913
BcgI CGANNNNNNTGC 2 cut(s) 547, 581
BciT130I CCWGG 7 cut(s) 360, 507, 2125, 2609, 2721, 2750, 3424
BciVI GTATCC 2 cut(s) 1980, 2971
BclI TGATCA 1 cut(s) 2235
BcnI CCSGG 3 cut(s) 13, 2169, 4082
BcoDI GTCTC 8 cut(s) 330, 517, 1291, 2190, 2581, 2733, 3171, 3800
BfaI CTAG 9 cut(s) 482, 873, 1904, 2064, 2115, 2325, 2523, 3486, 4188
BfmI CTRYAG 3 cut(s) 2268, 2940, 3467
BfoI RGCGCY 2 cut(s) 458, 4074
BfrI CTTAAG 2 cut(s) 3479, 3679
BfuAI ACCTGC 2 cut(s) 2964, 3517
BfuI GTATCC 2 cut(s) 1980, 2971
BglII AGATCT 6 cut(s) 800, 1257, 1404, 2159, 2525, 2617
BlnI CCTAGG 1 cut(s) 2114
Bme18I GGWCC 7 cut(s) 301, 467, 920, 1410, 1613, 3278, 3782
BmeRI GACNNNNNGTC 1 cut(s) 1221
BmeT110I CYCGRG 2 cut(s) 1503, 2637
BmrI ACTGGG 3 cut(s) 158, 1437, 1695
BmtI GCTAGC 1 cut(s) 1907
BmuI ACTGGG 3 cut(s) 158, 1437, 1695
BpiI GAAGAC 2 cut(s) 898, 2751
BpmI CTGGAG 4 cut(s) 299, 489, 2757, 3288
Bpu10I CCTNAGC 3 cut(s) 699, 852, 3696
Bpu14I TTCGAA 1 cut(s) 562
BpuEI CTTGAG 4 cut(s) 2128, 2269, 2835, 3727
BpuMI CCSGG 3 cut(s) 13, 2169, 4082
BsaAI YACGTR 1 cut(s) 1919
BsaHI GRCGYC 1 cut(s) 4071
BsaI GGTCTC 5 cut(s) 330, 1291, 2190, 3171, 3800
BsaJI CCNNGG 5 cut(s) 358, 1413, 2114, 2607, 3979
BsaWI WCCGGW 1 cut(s) 437
BsaXI ACNNNNNCTCC 2 cut(s) 2588, 2618
Bse118I RCCGGY 2 cut(s) 404, 591
Bse3DI GCAATG 3 cut(s) 372, 1644, 4033
BseBI CCWGG 7 cut(s) 360, 507, 2125, 2609, 2721, 2750, 3424
BseDI CCNNGG 5 cut(s) 358, 1413, 2114, 2607, 3979
BseMI GCAATG 3 cut(s) 372, 1644, 4033
BseRI GAGGAG 9 cut(s) 145, 177, 492, 1526, 1692, 2232, 3079, 3369, 3884
BseSI GKGCMC 1 cut(s) 200
BseYI CCCAGC 4 cut(s) 82, 395, 569, 3594
BsgI GTGCAG 3 cut(s) 1974, 3714, 4127
Bsh1236I CGCG 2 cut(s) 1633, 1839
Bsh1285I CGRYCG 1 cut(s) 4111
BshNI GGYRCC 5 cut(s) 223, 401, 3505, 3814, 4070
BsiEI CGRYCG 1 cut(s) 4111
BsiHKAI GWGCWC 3 cut(s) 445, 817, 2334
BsiHKCI CYCGRG 2 cut(s) 1503, 2637
BsiSI CCGG 6 cut(s) 12, 405, 438, 592, 2168, 4082
BslFI GGGAC 2 cut(s) 152, 1702
BsmAI GTCTC 8 cut(s) 330, 517, 1291, 2190, 2581, 2733, 3171, 3800
BsmBI CGTCTC 1 cut(s) 2733
BsmFI GGGAC 2 cut(s) 152, 1702
BsmI GAATGC 5 cut(s) 570, 1517, 1588, 2648, 3157
Bso31I GGTCTC 5 cut(s) 330, 1291, 2190, 3171, 3800
BsoBI CYCGRG 2 cut(s) 1503, 2637
Bsp119I TTCGAA 1 cut(s) 562
Bsp120I GGGCCC 1 cut(s) 196
Bsp1286I GDGCHC 6 cut(s) 200, 431, 445, 817, 2334, 3136
Bsp1407I TGTACA 2 cut(s) 1597, 1735
BspFNI CGCG 2 cut(s) 1633, 1839
BspMAI CTGCAG 2 cut(s) 2272, 2944
BspMI ACCTGC 2 cut(s) 2964, 3517
BspOI GCTAGC 1 cut(s) 1907
BspPI GGATC 7 cut(s) 455, 689, 702, 1613, 1878, 3818, 3831
BspT104I TTCGAA 1 cut(s) 562
BspT107I GGYRCC 5 cut(s) 223, 401, 3505, 3814, 4070
BspTI CTTAAG 2 cut(s) 3479, 3679
BspTNI GGTCTC 5 cut(s) 330, 1291, 2190, 3171, 3800
BsrDI GCAATG 3 cut(s) 372, 1644, 4033
BsrFI RCCGGY 2 cut(s) 404, 591
BsrGI TGTACA 2 cut(s) 1597, 1735
BssAI RCCGGY 2 cut(s) 404, 591
BssECI CCNNGG 5 cut(s) 358, 1413, 2114, 2607, 3979
BssNI GRCGYC 1 cut(s) 4071
BssSI CACGAG 2 cut(s) 1005, 3732
BssT1I CCWWGG 2 cut(s) 2114, 3979
Bst2BI CACGAG 2 cut(s) 1005, 3732
Bst2UI CCWGG 7 cut(s) 360, 507, 2125, 2609, 2721, 2750, 3424
Bst6I CTCTTC 2 cut(s) 1481, 3141
BstACI GRCGYC 1 cut(s) 4071
BstAFI CTTAAG 2 cut(s) 3479, 3679
BstAPI GCANNNNNTGC 1 cut(s) 2948
BstAUI TGTACA 2 cut(s) 1597, 1735
BstBAI YACGTR 1 cut(s) 1919
BstBI TTCGAA 1 cut(s) 562
BstFNI CGCG 2 cut(s) 1633, 1839
BstH2I RGCGCY 2 cut(s) 458, 4074
BstHHI GCGC 5 cut(s) 457, 1635, 3439, 3749, 4073
BstMAI GTCTC 8 cut(s) 330, 517, 1291, 2190, 2581, 2733, 3171, 3800
BstMCI CGRYCG 1 cut(s) 4111
BstNI CCWGG 7 cut(s) 360, 507, 2125, 2609, 2721, 2750, 3424
BstNSI RCATGY 3 cut(s) 58, 4157, 4211
BstSFI CTRYAG 3 cut(s) 2268, 2940, 3467
BstSLI GKGCMC 1 cut(s) 200
BstUI CGCG 2 cut(s) 1633, 1839
BstV2I GAAGAC 2 cut(s) 898, 2751
BstX2I RGATCY 9 cut(s) 694, 800, 1257, 1404, 1870, 2159, 2525, 2617, 3823
BstXI CCANNNNNNTGG 3 cut(s) 359, 1225, 4228
BstYI RGATCY 9 cut(s) 694, 800, 1257, 1404, 1870, 2159, 2525, 2617, 3823
BsuI GTATCC 2 cut(s) 1980, 2971
BtgZI GCGATG 2 cut(s) 1854, 4115
BtsI GCAGTG 4 cut(s) 415, 789, 2277, 2710
BveI ACCTGC 2 cut(s) 2964, 3517
CaiI CAGNNNCTG 3 cut(s) 1744, 2948, 2995
CfoI GCGC 5 cut(s) 457, 1635, 3439, 3749, 4073
Cfr10I RCCGGY 2 cut(s) 404, 591
DinI GGCGCC 1 cut(s) 4072
DraI TTTAAA 2 cut(s) 70, 3108
DrdI GACNNNNNNGTC 3 cut(s) 1195, 2204, 3233
DriI GACNNNNNGTC 1 cut(s) 1221
DseDI GACNNNNNNGTC 3 cut(s) 1195, 2204, 3233
EaeI YGGCCR 3 cut(s) 712, 1746, 2967
Eam1104I CTCTTC 2 cut(s) 1481, 3141
Eam1105I GACNNNNNGTC 1 cut(s) 1221
EarI CTCTTC 2 cut(s) 1481, 3141
EciI GGCGGA 1 cut(s) 12
Ecl136II GAGCTC 1 cut(s) 815
Eco130I CCWWGG 2 cut(s) 2114, 3979
Eco24I GRGCYC 4 cut(s) 200, 431, 817, 3136
Eco31I GGTCTC 5 cut(s) 330, 1291, 2190, 3171, 3800
Eco32I GATATC 1 cut(s) 4021
Eco47I GGWCC 7 cut(s) 301, 467, 920, 1410, 1613, 3278, 3782
Eco53kI GAGCTC 1 cut(s) 815
Eco57I CTGAAG 3 cut(s) 1875, 2430, 3483
Eco88I CYCGRG 2 cut(s) 1503, 2637
EcoICRI GAGCTC 1 cut(s) 815
EcoO109I RGGNCCY 1 cut(s) 197
EcoRI GAATTC 1 cut(s) 3652
EcoRII CCWGG 7 cut(s) 358, 505, 2123, 2607, 2719, 2748, 3422
EcoRV GATATC 1 cut(s) 4021
EcoT14I CCWWGG 2 cut(s) 2114, 3979
EcoT22I ATGCAT 1 cut(s) 4209
EcoT38I GRGCYC 4 cut(s) 200, 431, 817, 3136
EgeI GGCGCC 1 cut(s) 4072
EheI GGCGCC 1 cut(s) 4072
ErhI CCWWGG 2 cut(s) 2114, 3979
Esp3I CGTCTC 1 cut(s) 2733
FalI AAGNNNNNCTT 6 cut(s) 1539, 1571, 3066, 3098, 3972, 4004
FaqI GGGAC 2 cut(s) 152, 1702
FauI CCCGC 6 cut(s) 121, 177, 181, 219, 351, 4060
FbaI TGATCA 1 cut(s) 2235
FblI GTMKAC 1 cut(s) 1921
FriOI GRGCYC 4 cut(s) 200, 431, 817, 3136
FspBI CTAG 9 cut(s) 482, 873, 1904, 2064, 2115, 2325, 2523, 3486, 4188
GlaI GCGC 5 cut(s) 456, 1634, 3438, 3748, 4072
GsaI CCCAGC 4 cut(s) 86, 399, 573, 3598
GsuI CTGGAG 4 cut(s) 299, 489, 2757, 3288
HaeII RGCGCY 2 cut(s) 458, 4074
HapII CCGG 6 cut(s) 12, 405, 438, 592, 2168, 4082
HhaI GCGC 5 cut(s) 457, 1635, 3439, 3749, 4073
Hin1I GRCGYC 1 cut(s) 4071
Hin6I GCGC 5 cut(s) 455, 1633, 3437, 3747, 4071
HinP1I GCGC 5 cut(s) 455, 1633, 3437, 3747, 4071
HincII GTYRAC 6 cut(s) 108, 2428, 2473, 3376, 3397, 3704
HindII GTYRAC 6 cut(s) 108, 2428, 2473, 3376, 3397, 3704
HindIII AAGCTT 1 cut(s) 3495
HpaI GTTAAC 3 cut(s) 2473, 3376, 3397
HpaII CCGG 6 cut(s) 12, 405, 438, 592, 2168, 4082
Hpy99I CGWCG 1 cut(s) 196
HpyCH4IV ACGT 7 cut(s) 104, 612, 630, 1918, 2253, 3557, 3706
HpySE526I ACGT 7 cut(s) 104, 612, 630, 1918, 2253, 3557, 3706
Hsp92I GRCGYC 1 cut(s) 4071
HspAI GCGC 5 cut(s) 455, 1633, 3437, 3747, 4071
KasI GGCGCC 1 cut(s) 4070
KpnI GGTACC 2 cut(s) 3509, 3818
KroI GCCGGC 1 cut(s) 591
KroNI GCCGGC 1 cut(s) 593
Ksp22I TGATCA 1 cut(s) 2235
KspAI GTTAAC 3 cut(s) 2473, 3376, 3397
LmnI GCTCC 4 cut(s) 318, 1348, 2414, 2609
MaeI CTAG 9 cut(s) 482, 873, 1904, 2064, 2115, 2325, 2523, 3486, 4188
MaeII ACGT 7 cut(s) 104, 612, 630, 1918, 2253, 3557, 3706
MfeI CAATTG 2 cut(s) 1845, 3380
MflI RGATCY 9 cut(s) 694, 800, 1257, 1404, 1870, 2159, 2525, 2617, 3823
MhlI GDGCHC 6 cut(s) 200, 431, 445, 817, 2334, 3136
MlsI TGGCCA 2 cut(s) 1748, 2969
MluNI TGGCCA 2 cut(s) 1748, 2969
Mly113I GGCGCC 1 cut(s) 4071
Mox20I TGGCCA 2 cut(s) 1748, 2969
Mph1103I ATGCAT 1 cut(s) 4209
MroNI GCCGGC 1 cut(s) 591
MroXI GAANNNNTTC 1 cut(s) 996
MscI TGGCCA 2 cut(s) 1748, 2969
MslI CAYNNNNRTG 7 cut(s) 773, 836, 1462, 1895, 3587, 4152, 4226
Msp20I TGGCCA 2 cut(s) 1748, 2969
MspA1I CMGCKG 6 cut(s) 86, 399, 1958, 3284, 3728, 4009
MspCI CTTAAG 2 cut(s) 3479, 3679
MspI CCGG 6 cut(s) 12, 405, 438, 592, 2168, 4082
MunI CAATTG 2 cut(s) 1845, 3380
Mva1269I GAATGC 5 cut(s) 570, 1517, 1588, 2648, 3157
MvaI CCWGG 7 cut(s) 360, 507, 2125, 2609, 2721, 2750, 3424
MvnI CGCG 2 cut(s) 1633, 1839
NaeI GCCGGC 1 cut(s) 593
NarI GGCGCC 1 cut(s) 4071
NciI CCSGG 3 cut(s) 13, 2169, 4082
NgoMIV GCCGGC 1 cut(s) 591
NheI GCTAGC 1 cut(s) 1903
NmeAIII GCCGAG 2 cut(s) 1394, 2665
NmuCI GTSAC 8 cut(s) 829, 1186, 1336, 1542, 2707, 3362, 3667, 3763
NsiI ATGCAT 1 cut(s) 4209
NspI RCATGY 3 cut(s) 58, 4157, 4211
NspV TTCGAA 1 cut(s) 562
OliI CACNNNNGTG 2 cut(s) 1895, 3587
PcsI WCGNNNNNNNCGW 1 cut(s) 3045
PctI GAATGC 5 cut(s) 570, 1517, 1588, 2648, 3157
PdiI GCCGGC 1 cut(s) 593
PdmI GAANNNNTTC 1 cut(s) 996
PfoI TCCNGGA 1 cut(s) 2123
PluTI GGCGCC 1 cut(s) 4074
Ppu21I YACGTR 1 cut(s) 1919
Psp124BI GAGCTC 1 cut(s) 817
Psp6I CCWGG 7 cut(s) 358, 505, 2123, 2607, 2719, 2748, 3422
PspFI CCCAGC 4 cut(s) 82, 395, 569, 3594
PspGI CCWGG 7 cut(s) 358, 505, 2123, 2607, 2719, 2748, 3422
PspOMI GGGCCC 1 cut(s) 196
PstI CTGCAG 2 cut(s) 2272, 2944
PstNI CAGNNNCTG 3 cut(s) 1744, 2948, 2995
PsuI RGATCY 9 cut(s) 694, 800, 1257, 1404, 1870, 2159, 2525, 2617, 3823
PvuII CAGCTG 5 cut(s) 86, 1958, 3284, 3728, 4009
RseI CAYNNNNRTG 7 cut(s) 773, 836, 1462, 1895, 3587, 4152, 4226
SacI GAGCTC 1 cut(s) 817
SduI GDGCHC 6 cut(s) 200, 431, 445, 817, 2334, 3136
SfcI CTRYAG 3 cut(s) 2268, 2940, 3467
SfoI GGCGCC 1 cut(s) 4072
SfuI TTCGAA 1 cut(s) 562
SinI GGWCC 7 cut(s) 301, 467, 920, 1410, 1613, 3278, 3782
SmiMI CAYNNNNRTG 7 cut(s) 773, 836, 1462, 1895, 3587, 4152, 4226
SmlI CTYRAG 6 cut(s) 2107, 2284, 2850, 3479, 3679, 3742
SmoI CTYRAG 6 cut(s) 2107, 2284, 2850, 3479, 3679, 3742
SspDI GGCGCC 1 cut(s) 4070
SspMI CTAG 9 cut(s) 482, 873, 1904, 2064, 2115, 2325, 2523, 3486, 4188
SstI GAGCTC 1 cut(s) 817
StyI CCWWGG 2 cut(s) 2114, 3979
TaiI ACGT 7 cut(s) 107, 615, 633, 1921, 2256, 3560, 3709
TaqI TCGA 6 cut(s) 51, 425, 562, 663, 811, 1509
TaqII GACCGA 1 cut(s) 1205
TatI WGTACW 4 cut(s) 1063, 1597, 1735, 2027
TauI GCSGC 4 cut(s) 28, 402, 523, 717
TseFI GTSAC 8 cut(s) 829, 1186, 1336, 1542, 2707, 3362, 3667, 3763
Tsp45I GTSAC 8 cut(s) 829, 1186, 1336, 1542, 2707, 3362, 3667, 3763
TspGWI ACGGA 3 cut(s) 459, 2690, 4073
Vha464I CTTAAG 2 cut(s) 3479, 3679
VpaK11BI GGWCC 7 cut(s) 301, 467, 920, 1410, 1613, 3278, 3782
XapI RAATTY 6 cut(s) 909, 1607, 1652, 2845, 3429, 3652
XbaI TCTAGA 1 cut(s) 481
XceI RCATGY 3 cut(s) 58, 4157, 4211
XcmI CCANNNNNNNNNTGG 3 cut(s) 532, 1011, 2732
XmaJI CCTAGG 1 cut(s) 2114
XmiI GTMKAC 1 cut(s) 1921
XmnI GAANNNNTTC 1 cut(s) 996
XspI CTAG 9 cut(s) 482, 873, 1904, 2064, 2115, 2325, 2523, 3486, 4188
Zsp2I ATGCAT 1 cut(s) 4209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.