RLG00000009996

Enhancer of mRNA-decapping protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000324
Physical Location & Seq
Reverse (-)
61575595 .. 61581277
5683 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009996

Sequence Viewer

Length: 3951 bp
ATGGGTAAAGACATAGTGTATCAGGAGTCACCTGATGAACCCGGGAAACGCAGCAGAGTGTGGAGTGAAGAAGATGTAGACTACGTTCTAACAAATAATACAGGTACAAATAAAGTTATAGGCATTCAGTTGCCGTGGCTATCATCTACAATATCTTTGAATGCTAAAAGCTTTTCAGAGATGAAGAATCTTAGATATATTTCTATGAGAAGAATATCCTTTTCTGGAGATATTGATTATCTCTCCAACCAGTTGAGGTGGCTCGATTGGCAATCATGTCCGTTGCAATGTTTTCCATCCGATTTTCAAGCAAACAAACTTGTGCAGCTCAACATTAGTAGGGGCAAAATCAGACGACTATGGGAGGGACGAAAGAATTTTTCAAGGCTAACATGTATGAATTTAAGTGACTGTGAATCCCTAAAGGAACTCCCAGACTTGAGTGGAATCCCTAACTTGAAAGGGTTGAATTTAAGTGGATGTGTATCCCTAAAGGAACTCCCAGTCTTGAGTGGAATCCCTAACTTGAAAGAGTTGGATTTAAGTGGCTGTAAATCCCTAAAGGAACTCCCAGACTTGAGTGGAATCCCTAACTTGAAAAAGTTGGATTTAAGTGGCTGTAAATCCCTAAAGGAACTCCCAGACTTGAGTGGAATCCCTAACTTGAAAAAGTTGGATTTACGTGGCTGTACATCCCTAAAGGAACTCCCAGACTTGACTGGAATCCCTAACTTGAAAGGGTTGCATTTAAGTGGTTGTACATCCCTAAAGAAACTCCCAGACTTGAGTGGAATCCCTAACTTGAAAGGGTTGAATTTAAGTGGATGTGTATCCCTAAAGGAACTCCCAGTCTTGAGTGGAATCCCTAACTTGAAAGAGTTGAATTTAAGTGGCTGTAAATCCCTAAAGGAACTCCCAGACTTGAGTGGAATCCCTAACTTTTTCTCTAACTTGGAAGAGTTGAATTTAAGTGGCTGTGAATCCCTAAAGGAACTCCCAGACTTGAGTGGAATCCCTAACTTGAAAGAGTTGGATTTACGTGGCTGTACATCCCTAAACGAACTCTCAGACTTGAGTGGAATCCCTAACTTGAAAGGGTTGCATTTACGTGGCTGTACATCCCTAAAGAAACTCCCAGACTTGAGTGGAATCCCTAACTTGAAAGGGTTGAATTTAAGTGGTTGTGTATCCCTAAAGGAACTCCCAGACTTTTGTGGAATCCCTAACTTGAAAGAGTTGGAACTATATGAATGTATCAGTTTAGTTGAGGTTCCTGATTCTGTTGGATTTCTTGATAAGCTTGTTACCTTGAATGTTAGTGGTTGCTCTAGCCTTACTATGTTTCCAAGAAAAATCTATTGGAAATCCTTAGAAACAATCTCTATTAGCTATTGCAAGCTTGAAGAATTTTCAGAAGTTGGGGAAGAGATGGGTTTCTTGAGAAAATTAGATCTAAGAGGCACTTGCATTAAAGAATTGCATCCATCCATTACAAGGCTTATTAGGCTGGAAAAGTTGGACCTAACGAACAGTCAAAATCTTATAGCCCTGCCATATAATATTTATGAGTTGCACAATCTAAAGGCTCTTTATGTGAGAGGATGCTCAAAACTAGCTACGTTTCCAAAAATTCTAGTAAAGATGAATTCTTTGAGAGAACTCTCTCTTTCAGGCAGTGACATAAGAGAATTGGATAAGTCAATTGAAAATCTCATTGGGCTTGAATATTTGGATCTTACTGAATGCAAAAATCTCACAACTCTACCGTGCAGCATTTATGAGTTGCACAATCTAAAGAATCTTTTTGCGAGAGGATGCTTAAAACTGGCTACATTTCCAAAAATTCCAATAAAGATGGATTCCTTGATAATGCTCTCTCTTAAAGGCAGTGACATAGGAGAATTGGATGAGTCAATTGAAAATCTCATTGGGCTTGAATATTTGGATCTCAGTGATTGCAAAAATCTCACAACTCTACCGTGCAGCATTTATGGGTTGCAAAATTTATGGTTTCTTGATCTTAGTGAATGCTCAAAACTGGTCAGATTTCCAACAGATACAAAATTTTTGAATGACGATGGTTGCTCACTGTCACTTCCCAAGCTACGTGTGTTCAGTATCGCAGGGTGCAGTTTATTATCAGATTGTGATTTCCTGATGACTCTTGATTGCTGGGAAACATTAATTGGGCTTTATCTGTCAAGAAACAATTTTGTTAGTCTTCCTGCTTGCCTCACCAAATTTGTCAACTTGCGGGAGCTTTACTTGGAGGGTTGCAAGAGACTCCGAGAAATTCCGGAGCTTCCACCAAACGTTGAACCAAGAATAAGAGGTTGTGAATTACTGGAGGAGAGATTCTCGACATTGACAAATCGTGGGGCTGAGCCAATCCGAAACGGCACCGCACCGCGGACGAACCATCACCGAACCAACAAAACCTCGTGGCCTGATCCCATCATACTTATAACATTGGGCCCGCTTAATCGGGAAGTGAAGATCTGGTCCTCAGCGAGTGAAGAAGGCTGGCTACTTCCCAGTGATGCTGAGTCATGGAAATGTACCCAGACCTTAGAGTTAAAGGGCTCTGCTCAACCTCGAGTTGAGGATGCATTCTTTAATCAAGTCATAGCATTGTCACAAGCTGGCCTTCTTTTAGTTGCAAATGCTAAGAAGAATGCTATATTTGCCGTGCACTTAGACCTTGGTGGTGAGCCAGCATCTACATGCATGGATTACAAAGCAGAATTTACTGTTGCAATGCTTATTTTGAGCTTTACTGGGACAAGTATATCACCTCATGGTGAACAAATTTATTGTGTCCAAACACAAGCCATTCAACAGTATGCTATGGACTTATCCAAGTGTTTACCTCCCCTATTGGAGATTTCAGGTTTAGAAAAGTTCTTTTGTTTCCAGGCATCTGATCTTGGAATTGAGATGGCCTGGGATTGCTGTACAATATCATTAGAATCTTATATTACAGATGAAGCTCAGCAAGGTGATGGTGCCAGCATGTCAGAACCACTAGCCCAACCTCATTCTGAAGAGGAAGACCACGATCGCTCTACAAAAGATGTATCTGGGTCAGCTACCGCCACAACCGCATCACAACTACAAACACCATATGCAAAGTCGAGAAAACAGAAATGGAAAATTATGCAAGCATCAGGTCAATCTTCCCCATCACCAGGTGTGCTCAACTCAATAGAGCCCTCTAATGAAGCAGGTGGGAGTTCAAGTCCTCCCTCCGGAGAAGCTGCATTTCATCAAATTTTGGCCAGGCAGGATATGATGAATCAGCTAATGACTATGCAAAAAGAATTGCAAAAGCAGATGACAATGATGGTTACCGTCCCAGTTACAAAGGAAGGCAAACGATTAGAGGCGGCTCTCGGTCGGATGAATGCAAAGAATGAGAAATTATTGCGAGGCCGTACTCAGCAAGTAACTGGTTTGATTGTGAACAAGGACTTTCCAGTCATGTTAGAGAAAATGGTAAAAGAAGAAACAGCTGCTGTTGGACAAGCTGTTGTTCGTGCAATCACTCCAGATATGGAGAAAACAATAACTTCGGCTATATCTGATTGCTTTCAGAGAGGAGTGGGAGATAAGGCAGTTAATCAATTGGAAAAATCAGTTAACTCAAAACTTGATGCTACTGTATCCAGGCAAATCAAGGTGCAGTTTCAAACATCAGGTAAACAAGCTCTTCAAGATGCTCTCAAGTCTAGCATGGAAGCTTCAGTGGTACCTGCCTTTGAGACGTCATGTAAATCCATGTTTGAGCAAGTAGATGCCACATTCCAGAAAGGAATGGTTGAACATGCAACTGTGGCTCAGCAGCAATTTGAGTCTGCACATTCGCCGTTGGCCCATGCTGAAGCTATTAGCTTCACATCATCAGAGACTCAAATCCTAAGCGGTGAACTGGCTAATGGCAACGTAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000932 GO:0000956 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009892 GO:0009933 GO:0009965 GO:0009987 GO:0010014 GO:0010015 GO:0010016 GO:0010033 GO:0010071 GO:0010072 GO:0010154 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0022414 GO:0022622 GO:0023052 GO:0031086 GO:0031087 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0042221 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046700 GO:0046983 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048532 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071704 GO:0090304 GO:0090305 GO:0090421 GO:0097159 GO:0099402 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
Ge1_WD40

Protein Analysis

1317

Amino Acids

145.22

Weight (kDa)

5.66

Isoelectric Point (pI)

34.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 174 - 217 1.7e-07 Leucine Rich repeats (2 copies)
LRR_4 PF12799 220 - 263 4.5e-06 Leucine Rich repeats (2 copies)
LRR_4 PF12799 266 - 309 1.1e-06 Leucine Rich repeats (2 copies)
LRR_R13L1-DRL21 PF25019 285 - 349 7.3e-07 R13L1/DRL21 LRRs
LRR_RPS2 PF23247 291 - 423 8.3e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_4 PF12799 317 - 359 2.3e-06 Leucine Rich repeats (2 copies)
LRR_13 PF23286 455 - 538 8.3e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 480 - 557 3.5e-09 Leucine-rich repeat region
LRR_14 PF23598 545 - 627 5.5e-08 Leucine-rich repeat region
LRR_13 PF23286 601 - 767 3.7e-17 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13290 AT3G13300 AT3G13300 AT3G13300
fragaria_vesca FvH4_3g41972 FvH4_3g41980 FvH4_3g41980 FvH4_3g41980 FvH4_3g41980 FvH4_3g42780 FvH4_3g42790 FvH4_3g42820 FvH4_3g42820 FvH4_3g42820 FvH4_3g42820 FvH4_4g01881 FvH4_7g10420
malus_domestica MD00G1013700.v1.1 MD03G1039800.v1.1 MD11G1041300.v1.1 MD11G1094900.v1.1
prunus_persica Prupe.6G032100_v2.0.a1
pyrus_communis pycom03g03070 pycom11g03500 pycom11g08470 pycom12g06730
rosa_chinensis RchiOBHm_Chr1g0328831 RchiOBHm_Chr4g0386471 RchiOBHm_Chr4g0386481 RchiOBHm_Chr4g0386551 RchiOBHm_Chr4g0386701 RchiOBHm_Chr4g0386801 RchiOBHm_Chr4g0386811 RchiOBHm_Chr4g0386821 RchiOBHm_Chr4g0386921 RchiOBHm_Chr4g0386931 RchiOBHm_Chr4g0386941 RchiOBHm_Chr4g0387211 RchiOBHm_Chr4g0389101 RchiOBHm_Chr4g0389111 RchiOBHm_Chr4g0389201 RchiOBHm_Chr4g0389211 RchiOBHm_Chr4g0389321 RchiOBHm_Chr4g0389331 RchiOBHm_Chr4g0389341 RchiOBHm_Chr4g0389411 RchiOBHm_Chr5g0075171
rosa_laevigata RLG00000009985 RLG00000009990 RLG00000009996 RLG00000009998 RLG00000010136 RLG00000010140 RLG00000036537
rosa_multiflora Rmu_co8461959.1_g000001 Rmu_sc0000013.1_g000017 Rmu_sc0000013.1_g000024 Rmu_sc0001015.1_g000001 Rmu_sc0001395.1_g000004 Rmu_sc0001942.1_g000022 Rmu_sc0002498.1_g000018 Rmu_sc0002498.1_g000020 Rmu_sc0002557.1_g000005 Rmu_sc0002604.1_g000010 Rmu_sc0002604.1_g000011 Rmu_sc0004750.1_g000003 Rmu_sc0006571.1_g000001 Rmu_sc0006571.1_g000014 Rmu_sc0006571.1_g000018 Rmu_sc0008050.1_g000016 Rmu_sc0008066.1_g000009 Rmu_sc0008380.1_g000006 Rmu_sc0008380.1_g000010 Rmu_sc0008380.1_g000012 Rmu_sc0010272.1_g000021 Rmu_sc0010693.1_g000010 Rmu_sc0010693.1_g000012 Rmu_sc0010693.1_g000014 Rmu_sc0010693.1_g000015 Rmu_sc0031790.1_g000003 Rmu_sc0040539.1_g000001 Rmu_ssc0000211.1_g000001 Rmu_ssc0000211.1_g000011 Rmu_ssc0000211.1_g000013
rosa_roxburghii Rroxscaffold_1G00006420 Rroxscaffold_1G00006430 Rroxscaffold_5G00334020 Rroxscaffold_5G00334050 Rroxscaffold_5G00335420
rosa_rugosa Rorug03G0307600 Rorug03G0308600 Rorug03G0322400 Rorug05G0437200 Rorug05G0437200 Rorug05G0437200
rosa_samantha Rh1BG075700 Rh4BG010000 Rh4BG010100 Rh4BG010200 Rh4BG020000 Rh4BG020400 Rh4BG172100 Rh4CG015300 Rh4CG016300 Rh5AG494100 Rh5AG494200 Rh5BG515500 Rh5CG539300 Rh5DG528200
rosa_wichuraiana Rw4G000930 Rw4G001980 Rw4G001990 Rw4G011950 Rw5G045850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2465
AarI CACCTGC 1 cut(s) 3215
AasI GACNNNNNNGTC 1 cut(s) 3476
AatII GACGTC 1 cut(s) 3767
Acc36I ACCTGC 2 cut(s) 3215, 3760
Acc65I GGTACC 1 cut(s) 3748
AccB1I GGYRCC 3 cut(s) 2399, 3005, 3748
AccI GTMKAC 1 cut(s) 78
AccII CGCG 1 cut(s) 2410
AccIII TCCGGA 2 cut(s) 2296, 3248
AciI CCGC 9 cut(s) 2254, 2403, 2408, 2410, 2477, 3093, 3102, 3386, 3921
AclI AACGTT 1 cut(s) 2313
AclWI GGATC 3 cut(s) 1740, 1953, 2444
AcoI YGGCCR 1 cut(s) 3276
AcuI CTGAAG 3 cut(s) 3063, 3726, 3900
AcyI GRCGYC 1 cut(s) 3764
AdeI CACNNNGTG 1 cut(s) 3191
AfaI GTAC 9 cut(s) 106, 691, 760, 1048, 1117, 2560, 2956, 3436, 3750
AfiI CCNNNNNNNGG 4 cut(s) 1494, 2409, 3188, 3248
AflIII ACRYGT 2 cut(s) 392, 2107
AjnI CCWGG 5 cut(s) 2913, 2942, 3187, 3278, 3665
AjuI GAANNNNNNNTTGG 8 cut(s) 1342, 1374, 1698, 1730, 1911, 1943, 2169, 2201
Alw21I GWGCWC 2 cut(s) 2694, 3198
Alw26I GTCTC 3 cut(s) 2275, 3755, 3899
Alw44I GTGCAC 1 cut(s) 2690
AlwI GGATC 3 cut(s) 1740, 1953, 2444
AlwNI CAGNNNCTG 1 cut(s) 3515
Ama87I CYCGRG 2 cut(s) 41, 2595
Aor13HI TCCGGA 2 cut(s) 2296, 3248
AoxI GGCC 7 cut(s) 2444, 2473, 2644, 2940, 3276, 3430, 3870
ApaI GGGCCC 1 cut(s) 2477
ApaLI GTGCAC 1 cut(s) 2690
ApeKI GCWGC 7 cut(s) 51, 325, 1770, 1983, 3257, 3512, 3841
AseI ATTAAT 1 cut(s) 2183
Asp718I GGTACC 1 cut(s) 3748
AspS9I GGNCC 5 cut(s) 1519, 2473, 2474, 2502, 3871
AsuC2I CCSGG 2 cut(s) 42, 43
AsuHPI GGTGA 9 cut(s) 21, 2227, 2414, 2720, 2784, 2813, 3011, 3177, 3935
AvaI CYCGRG 2 cut(s) 41, 2595
AvaII GGWCC 2 cut(s) 1519, 2502
BaeGI GKGCMC 2 cut(s) 2477, 2694
BalI TGGCCA 1 cut(s) 3278
BanI GGYRCC 3 cut(s) 2399, 3005, 3748
BanII GRGCYC 3 cut(s) 2477, 2585, 3213
BarI GAAGNNNNNNTAC 2 cut(s) 2511, 2543
BauI CACGAG 1 cut(s) 2440
BbsI GAAGAC 2 cut(s) 2213, 3057
Bbv12I GWGCWC 2 cut(s) 2694, 3198
BbvCI CCTCAGC 1 cut(s) 2506
BbvI GCAGC 7 cut(s) 63, 337, 1782, 1995, 3244, 3499, 3853
BceAI ACGGC 5 cut(s) 118, 2413, 2672, 3417, 3850
BciT130I CCWGG 5 cut(s) 2915, 2944, 3189, 3280, 3667
BciVI GTATCC 4 cut(s) 496, 841, 1198, 3673
BcnI CCSGG 2 cut(s) 42, 43
BcoDI GTCTC 3 cut(s) 2275, 3755, 3899
BfaI CTAG 5 cut(s) 1329, 1613, 1634, 3026, 3729
BfuAI ACCTGC 2 cut(s) 3215, 3760
BfuI GTATCC 4 cut(s) 496, 841, 1198, 3673
BglII AGATCT 2 cut(s) 1450, 2496
BisI GCNGC 8 cut(s) 52, 326, 1771, 1984, 3258, 3387, 3513, 3842
BlpI GCTNAGC 3 cut(s) 2382, 2991, 3837
BlsI GCNGC 8 cut(s) 53, 327, 1772, 1985, 3259, 3388, 3514, 3843
Bme1390I CCNGG 7 cut(s) 42, 43, 2915, 2944, 3189, 3280, 3667
Bme18I GGWCC 2 cut(s) 1519, 2502
BmeT110I CYCGRG 2 cut(s) 41, 2595
BmgT120I GGNCC 5 cut(s) 1519, 2473, 2474, 2502, 3871
BmiI GGNNCC 5 cut(s) 1272, 2401, 2475, 3007, 3750
BmrFI CCNGG 7 cut(s) 42, 43, 2915, 2944, 3189, 3280, 3667
BmrI ACTGGG 5 cut(s) 497, 842, 2529, 2787, 3350
BmuI ACTGGG 5 cut(s) 497, 842, 2529, 2787, 3350
BpiI GAAGAC 2 cut(s) 2213, 3057
BplI GAGNNNNNCTC 2 cut(s) 2342, 2374
BpmI CTGGAG 3 cut(s) 246, 2366, 3531
Bpu10I CCTNAGC 2 cut(s) 2506, 3917
Bpu1102I GCTNAGC 3 cut(s) 2382, 2991, 3837
BpuMI CCSGG 2 cut(s) 42, 43
BsaAI YACGTR 4 cut(s) 683, 1040, 1109, 2108
BsaBI GATNNNNATC 2 cut(s) 484, 829
BsaHI GRCGYC 1 cut(s) 3764
BsaJI CCNNGG 5 cut(s) 41, 134, 2408, 2701, 2943
BsaWI WCCGGW 2 cut(s) 2296, 3248
BsaXI ACNNNNNCTCC 2 cut(s) 3223, 3253
Bsc4I CCNNNNNNNGG 4 cut(s) 1494, 2409, 3188, 3248
Bse3DI GCAATG 2 cut(s) 293, 2763
Bse8I GATNNNNATC 2 cut(s) 484, 829
BseAI TCCGGA 2 cut(s) 2296, 3248
BseBI CCWGG 5 cut(s) 2915, 2944, 3189, 3280, 3667
BseDI CCNNGG 5 cut(s) 41, 134, 2408, 2701, 2943
BseJI GATNNNNATC 2 cut(s) 484, 829
BseLI CCNNNNNNNGG 4 cut(s) 1494, 2409, 3188, 3248
BseMI GCAATG 2 cut(s) 293, 2763
BseMII CTCAG 8 cut(s) 1080, 1963, 2373, 2520, 2535, 3005, 3452, 3851
BseRI GAGGAG 2 cut(s) 2363, 3612
BseSI GKGCMC 2 cut(s) 2477, 2694
BseXI GCAGC 7 cut(s) 63, 337, 1782, 1995, 3244, 3499, 3853
BseYI CCCAGC 1 cut(s) 2172
BsgI GTGCAG 6 cut(s) 344, 1789, 2002, 2149, 3701, 3840
Bsh1236I CGCG 1 cut(s) 2410
Bsh1285I CGRYCG 2 cut(s) 3061, 3397
BshFI GGCC 7 cut(s) 2446, 2475, 2646, 2942, 3278, 3432, 3872
BshNI GGYRCC 3 cut(s) 2399, 3005, 3748
BsiEI CGRYCG 2 cut(s) 3061, 3397
BsiHKAI GWGCWC 2 cut(s) 2694, 3198
BsiHKCI CYCGRG 2 cut(s) 41, 2595
BsiSI CCGG 3 cut(s) 42, 2297, 3249
BslFI GGGAC 3 cut(s) 381, 2794, 3338
BslI CCNNNNNNNGG 4 cut(s) 1494, 2409, 3188, 3248
BsmAI GTCTC 3 cut(s) 2275, 3755, 3899
BsmBI CGTCTC 1 cut(s) 3755
BsmFI GGGAC 3 cut(s) 381, 2794, 3338
BsmI GAATGC 7 cut(s) 123, 166, 1748, 2033, 2609, 2680, 3409
BsnI GGCC 7 cut(s) 2446, 2475, 2646, 2942, 3278, 3432, 3872
BsoBI CYCGRG 2 cut(s) 41, 2595
Bsp120I GGGCCC 1 cut(s) 2473
Bsp1286I GDGCHC 5 cut(s) 2477, 2585, 2694, 3198, 3213
Bsp13I TCCGGA 2 cut(s) 2296, 3248
Bsp1407I TGTACA 5 cut(s) 689, 758, 1046, 1115, 2954
Bsp143I GATC 8 cut(s) 1450, 1732, 1945, 2017, 2449, 2496, 2923, 3058
Bsp1720I GCTNAGC 3 cut(s) 2382, 2991, 3837
BspACI CCGC 9 cut(s) 2254, 2403, 2408, 2410, 2477, 3093, 3102, 3386, 3921
BspANI GGCC 7 cut(s) 2446, 2475, 2646, 2942, 3278, 3432, 3872
BspCNI CTCAG 8 cut(s) 1079, 1962, 2374, 2519, 2536, 3004, 3451, 3850
BspEI TCCGGA 2 cut(s) 2296, 3248
BspFNI CGCG 1 cut(s) 2410
BspLI GGNNCC 5 cut(s) 1272, 2401, 2475, 3007, 3750
BspMI ACCTGC 2 cut(s) 3215, 3760
BspPI GGATC 3 cut(s) 1740, 1953, 2444
BspQI GCTCTTC 1 cut(s) 3714
BspT107I GGYRCC 3 cut(s) 2399, 3005, 3748
BsrDI GCAATG 2 cut(s) 293, 2763
BsrGI TGTACA 5 cut(s) 689, 758, 1046, 1115, 2954
BssECI CCNNGG 5 cut(s) 41, 134, 2408, 2701, 2943
BssMI GATC 8 cut(s) 1450, 1732, 1945, 2017, 2449, 2496, 2923, 3058
BssNI GRCGYC 1 cut(s) 3764
BssSI CACGAG 1 cut(s) 2440
BssT1I CCWWGG 1 cut(s) 2701
Bst2BI CACGAG 1 cut(s) 2440
Bst2UI CCWGG 5 cut(s) 2915, 2944, 3189, 3280, 3667
Bst6I CTCTTC 4 cut(s) 951, 1419, 3039, 3714
BstACI GRCGYC 1 cut(s) 3764
BstAUI TGTACA 5 cut(s) 689, 758, 1046, 1115, 2954
BstBAI YACGTR 4 cut(s) 683, 1040, 1109, 2108
BstC8I GCNNGC 8 cut(s) 1397, 2230, 2477, 2525, 2644, 2715, 3010, 3162
BstDSI CCRYGG 2 cut(s) 134, 2408
BstEII GGTNACC 1 cut(s) 3346
BstFNI CGCG 1 cut(s) 2410
BstKTI GATC 8 cut(s) 1453, 1735, 1948, 2020, 2452, 2499, 2926, 3061
BstMAI GTCTC 3 cut(s) 2275, 3755, 3899
BstMBI GATC 8 cut(s) 1450, 1732, 1945, 2017, 2449, 2496, 2923, 3058
BstMCI CGRYCG 2 cut(s) 3061, 3397
BstMWI GCNNNNNNNGC 5 cut(s) 268, 1504, 2684, 3101, 3833
BstNI CCWGG 5 cut(s) 2915, 2944, 3189, 3280, 3667
BstNSI RCATGY 4 cut(s) 396, 2727, 3016, 3827
BstPI GGTNACC 1 cut(s) 3346
BstSCI CCNGG 7 cut(s) 40, 41, 2913, 2942, 3187, 3278, 3665
BstSLI GKGCMC 2 cut(s) 2477, 2694
BstUI CGCG 1 cut(s) 2410
BstV1I GCAGC 7 cut(s) 63, 337, 1782, 1995, 3244, 3499, 3853
BstV2I GAAGAC 2 cut(s) 2213, 3057
BstX2I RGATCY 4 cut(s) 1450, 1732, 1945, 2496
BstYI RGATCY 4 cut(s) 1450, 1732, 1945, 2496
BsuI GTATCC 4 cut(s) 496, 841, 1198, 3673
BsuRI GGCC 7 cut(s) 2446, 2475, 2646, 2942, 3278, 3432, 3872
BtgI CCRYGG 2 cut(s) 134, 2408
BtsI GCAGTG 2 cut(s) 1681, 1894
BtsIMutI CAGTG 6 cut(s) 1681, 1894, 1957, 2087, 2542, 3750
BveI ACCTGC 2 cut(s) 3215, 3760
Cac8I GCNNGC 8 cut(s) 1397, 2230, 2477, 2525, 2644, 2715, 3010, 3162
CaiI CAGNNNCTG 1 cut(s) 3515
Cfr13I GGNCC 5 cut(s) 1519, 2473, 2474, 2502, 3871
Cfr42I CCGCGG 1 cut(s) 2411
Cfr9I CCCGGG 1 cut(s) 41
CsiI ACCWGGT 1 cut(s) 3187
Csp6I GTAC 9 cut(s) 105, 690, 759, 1047, 1116, 2559, 2955, 3435, 3749
CviQI GTAC 9 cut(s) 105, 690, 759, 1047, 1116, 2559, 2955, 3435, 3749
DpnI GATC 8 cut(s) 1452, 1734, 1947, 2019, 2451, 2498, 2925, 3060
DpnII GATC 8 cut(s) 1450, 1732, 1945, 2017, 2449, 2496, 2923, 3058
DraIII CACNNNGTG 1 cut(s) 3191
DrdI GACNNNNNNGTC 1 cut(s) 3476
DseDI GACNNNNNNGTC 1 cut(s) 3476
EaeI YGGCCR 1 cut(s) 3276
Eam1104I CTCTTC 4 cut(s) 951, 1419, 3039, 3714
EarI CTCTTC 4 cut(s) 951, 1419, 3039, 3714
Eco130I CCWWGG 1 cut(s) 2701
Eco24I GRGCYC 3 cut(s) 2477, 2585, 3213
Eco47I GGWCC 2 cut(s) 1519, 2502
Eco57I CTGAAG 3 cut(s) 3063, 3726, 3900
Eco88I CYCGRG 2 cut(s) 41, 2595
Eco91I GGTNACC 1 cut(s) 3346
EcoO65I GGTNACC 1 cut(s) 3346
EcoRI GAATTC 1 cut(s) 1645
EcoRII CCWGG 5 cut(s) 2913, 2942, 3187, 3278, 3665
EcoT14I CCWWGG 1 cut(s) 2701
EcoT22I ATGCAT 2 cut(s) 2611, 2729
EcoT38I GRGCYC 3 cut(s) 2477, 2585, 3213
ErhI CCWWGG 1 cut(s) 2701
Esp3I CGTCTC 1 cut(s) 3755
FalI AAGNNNNNCTT 4 cut(s) 1447, 1479, 2631, 2663
FaqI GGGAC 3 cut(s) 381, 2794, 3338
FauI CCCGC 2 cut(s) 2247, 2484
FauNDI CATATG 1 cut(s) 3124
FblI GTMKAC 1 cut(s) 78
Fnu4HI GCNGC 8 cut(s) 52, 326, 1771, 1984, 3258, 3387, 3513, 3842
FriOI GRGCYC 3 cut(s) 2477, 2585, 3213
Fsp4HI GCNGC 8 cut(s) 52, 326, 1771, 1984, 3258, 3387, 3513, 3842
FspBI CTAG 5 cut(s) 1329, 1613, 1634, 3026, 3729
GluI GCNGC 8 cut(s) 52, 326, 1771, 1984, 3258, 3387, 3513, 3842
GsaI CCCAGC 1 cut(s) 2176
GsuI CTGGAG 3 cut(s) 246, 2366, 3531
HaeIII GGCC 7 cut(s) 2446, 2475, 2646, 2942, 3278, 3432, 3872
HapII CCGG 3 cut(s) 42, 2297, 3249
Hin1I GRCGYC 1 cut(s) 3764
HincII GTYRAC 2 cut(s) 2248, 3640
HindII GTYRAC 2 cut(s) 2248, 3640
HindIII AAGCTT 4 cut(s) 169, 1298, 1397, 3738
HpaI GTTAAC 1 cut(s) 3640
HpaII CCGG 3 cut(s) 42, 2297, 3249
HphI GGTGA 9 cut(s) 21, 2227, 2414, 2720, 2784, 2813, 3011, 3177, 3935
Hpy166II GTNNAC 9 cut(s) 79, 2248, 2692, 2804, 2867, 3463, 3640, 3701, 3926
Hpy8I GTNNAC 9 cut(s) 79, 2248, 2692, 2804, 2867, 3463, 3640, 3701, 3926
HpyAV CCTTC 3 cut(s) 2513, 2657, 3362
HpyCH4IV ACGT 9 cut(s) 84, 682, 1039, 1108, 1619, 2107, 2313, 3764, 3942
HpyF10VI GCNNNNNNNGC 5 cut(s) 268, 1504, 2684, 3101, 3833
HpySE526I ACGT 9 cut(s) 84, 682, 1039, 1108, 1619, 2107, 2313, 3764, 3942
Hsp92I GRCGYC 1 cut(s) 3764
Kpn2I TCCGGA 2 cut(s) 2296, 3248
KpnI GGTACC 1 cut(s) 3752
KspAI GTTAAC 1 cut(s) 3640
KspI CCGCGG 1 cut(s) 2411
Kzo9I GATC 8 cut(s) 1450, 1732, 1945, 2017, 2449, 2496, 2923, 3058
LguI GCTCTTC 1 cut(s) 3714
LmnI GCTCC 2 cut(s) 2257, 2299
Lsp1109I GCAGC 7 cut(s) 63, 337, 1782, 1995, 3244, 3499, 3853
MabI ACCWGGT 1 cut(s) 3187
MaeI CTAG 5 cut(s) 1329, 1613, 1634, 3026, 3729
MaeII ACGT 9 cut(s) 84, 682, 1039, 1108, 1619, 2107, 2313, 3764, 3942
MalI GATC 8 cut(s) 1452, 1734, 1947, 2019, 2451, 2498, 2925, 3060
MboI GATC 8 cut(s) 1450, 1732, 1945, 2017, 2449, 2496, 2923, 3058
MfeI CAATTG 3 cut(s) 1701, 1914, 3623
MflI RGATCY 4 cut(s) 1450, 1732, 1945, 2496
MhlI GDGCHC 5 cut(s) 2477, 2585, 2694, 3198, 3213
MlsI TGGCCA 1 cut(s) 3278
MluNI TGGCCA 1 cut(s) 3278
MlyI GAGTC 7 cut(s) 35, 1919, 2155, 2277, 2555, 3860, 3901
Mox20I TGGCCA 1 cut(s) 3278
Mph1103I ATGCAT 2 cut(s) 2611, 2729
MroI TCCGGA 2 cut(s) 2296, 3248
MscI TGGCCA 1 cut(s) 3278
MslI CAYNNNNRTG 4 cut(s) 750, 1107, 2554, 2722
Msp20I TGGCCA 1 cut(s) 3278
MspA1I CMGCKG 2 cut(s) 2410, 3512
MspI CCGG 3 cut(s) 42, 2297, 3249
MspR9I CCNGG 7 cut(s) 42, 43, 2915, 2944, 3189, 3280, 3667
MunI CAATTG 3 cut(s) 1701, 1914, 3623
Mva1269I GAATGC 7 cut(s) 123, 166, 1748, 2033, 2609, 2680, 3409
MvaI CCWGG 5 cut(s) 2915, 2944, 3189, 3280, 3667
MvnI CGCG 1 cut(s) 2410
MwoI GCNNNNNNNGC 5 cut(s) 268, 1504, 2684, 3101, 3833
NciI CCSGG 2 cut(s) 42, 43
NdeI CATATG 1 cut(s) 3124
NdeII GATC 8 cut(s) 1450, 1732, 1945, 2017, 2449, 2496, 2923, 3058
NlaIV GGNNCC 5 cut(s) 1272, 2401, 2475, 3007, 3750
NmuCI GTSAC 6 cut(s) 27, 407, 1676, 1889, 2091, 2634
NsiI ATGCAT 2 cut(s) 2611, 2729
NspI RCATGY 4 cut(s) 396, 2727, 3016, 3827
PaeR7I CTCGAG 1 cut(s) 2595
PaqCI CACCTGC 1 cut(s) 3215
PciI ACATGT 1 cut(s) 392
PciSI GCTCTTC 1 cut(s) 3714
PctI GAATGC 7 cut(s) 123, 166, 1748, 2033, 2609, 2680, 3409
PkrI GCNGC 8 cut(s) 53, 327, 1772, 1985, 3259, 3388, 3514, 3843
Ple19I CGATCG 1 cut(s) 3061
PleI GAGTC 7 cut(s) 34, 1918, 2155, 2277, 2554, 3859, 3901
PpsI GAGTC 7 cut(s) 34, 1918, 2155, 2277, 2554, 3859, 3901
Ppu21I YACGTR 4 cut(s) 683, 1040, 1109, 2108
PscI ACATGT 1 cut(s) 392
PshBI ATTAAT 1 cut(s) 2183
PsiI TTATAA 1 cut(s) 2465
Psp1406I AACGTT 1 cut(s) 2313
Psp6I CCWGG 5 cut(s) 2913, 2942, 3187, 3278, 3665
PspEI GGTNACC 1 cut(s) 3346
PspFI CCCAGC 1 cut(s) 2172
PspGI CCWGG 5 cut(s) 2913, 2942, 3187, 3278, 3665
PspN4I GGNNCC 5 cut(s) 1272, 2401, 2475, 3007, 3750
PspOMI GGGCCC 1 cut(s) 2473
PspPI GGNCC 5 cut(s) 1519, 2473, 2474, 2502, 3871
PspXI VCTCGAGB 1 cut(s) 2595
PsrI GAACNNNNNNTAC 2 cut(s) 69, 101
PstNI CAGNNNCTG 1 cut(s) 3515
PsuI RGATCY 4 cut(s) 1450, 1732, 1945, 2496
PvuI CGATCG 1 cut(s) 3061
PvuII CAGCTG 1 cut(s) 3512
RsaI GTAC 9 cut(s) 106, 691, 760, 1048, 1117, 2560, 2956, 3436, 3750
RsaNI GTAC 9 cut(s) 105, 690, 759, 1047, 1116, 2559, 2955, 3435, 3749
RseI CAYNNNNRTG 4 cut(s) 750, 1107, 2554, 2722
SacII CCGCGG 1 cut(s) 2411
SapI GCTCTTC 1 cut(s) 3714
SatI GCNGC 8 cut(s) 52, 326, 1771, 1984, 3258, 3387, 3513, 3842
Sau3AI GATC 8 cut(s) 1450, 1732, 1945, 2017, 2449, 2496, 2923, 3058
Sau96I GGNCC 5 cut(s) 1519, 2473, 2474, 2502, 3871
SchI GAGTC 7 cut(s) 35, 1919, 2155, 2277, 2555, 3860, 3901
ScrFI CCNGG 7 cut(s) 42, 43, 2915, 2944, 3189, 3280, 3667
SduI GDGCHC 5 cut(s) 2477, 2585, 2694, 3198, 3213
SexAI ACCWGGT 1 cut(s) 3187
Sfr274I CTCGAG 1 cut(s) 2595
Sfr303I CCGCGG 1 cut(s) 2411
SgrBI CCGCGG 1 cut(s) 2411
SinI GGWCC 2 cut(s) 1519, 2502
SlaI CTCGAG 1 cut(s) 2595
SmaI CCCGGG 1 cut(s) 43
SmiMI CAYNNNNRTG 4 cut(s) 750, 1107, 2554, 2722
SsiI CCGC 9 cut(s) 2254, 2403, 2408, 2410, 2477, 3093, 3102, 3386, 3921
SspI AATATT 3 cut(s) 1561, 1727, 1940
SspMI CTAG 5 cut(s) 1329, 1613, 1634, 3026, 3729
StyD4I CCNGG 7 cut(s) 40, 41, 2913, 2942, 3187, 3278, 3665
StyI CCWWGG 1 cut(s) 2701
TaiI ACGT 9 cut(s) 87, 685, 1042, 1111, 1622, 2110, 2316, 3767, 3945
TaqI TCGA 4 cut(s) 264, 2360, 2596, 3134
TaqII GACCGA 1 cut(s) 3383
TatI WGTACW 5 cut(s) 689, 758, 1046, 1115, 2954
TauI GCSGC 1 cut(s) 3389
TscAI CASTG 6 cut(s) 1681, 1894, 1957, 2094, 2542, 3750
TseFI GTSAC 6 cut(s) 27, 407, 1676, 1889, 2091, 2634
TseI GCWGC 7 cut(s) 51, 325, 1770, 1983, 3257, 3512, 3841
Tsp45I GTSAC 6 cut(s) 27, 407, 1676, 1889, 2091, 2634
TspGWI ACGGA 1 cut(s) 270
TspMI CCCGGG 1 cut(s) 41
TspRI CASTG 6 cut(s) 1681, 1894, 1957, 2094, 2542, 3750
VneI GTGCAC 1 cut(s) 2690
VpaK11BI GGWCC 2 cut(s) 1519, 2502
VspI ATTAAT 1 cut(s) 2183
XceI RCATGY 4 cut(s) 396, 2727, 3016, 3827
XhoI CTCGAG 1 cut(s) 2595
XmaI CCCGGG 1 cut(s) 41
XmiI GTMKAC 1 cut(s) 78
XspI CTAG 5 cut(s) 1329, 1613, 1634, 3026, 3729
ZraI GACGTC 1 cut(s) 3765
Zsp2I ATGCAT 2 cut(s) 2611, 2729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.