Rw5G045850

Enhancer of mRNA-decapping protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000324
Physical Location & Seq
Reverse (-)
79856838 .. 79864071
7234 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G045850.1

Sequence Viewer

Length: 4185 bp
ATGGCGTCCCCCGGAAACCCCAATCCGCCGCCGCCGCAGCAGCAGCAGCCCTTCGACATGGCCAAGTTCTTTAAACCCACAAGCCCAACCCCTCTCTCCCCCCATTCCCAGCCTCAGACCCAGGCCCCCACCCAAAACCCTAACCCTAATCTGAACTCCTCAGGCTTCCCAATCCCTCCGGCCTCTTACCCTCCCCCCTCTGGGCCCTACTCCTACCCTCCCCAAACGGCGCCGTATCACCAGCAGCAATTCCAGTACCAGCACCACCCTCAGCCTCATCCTCACAACCACCCCCAAATTCCTTACCCTCAAGACCACCAGGTGCTTCAGCAGCGGTCGCTCTCGTTTCCCATCCCGCCCCTGGCTCCCGGTAATTACAACATTGCCGCCGCCGCCGCCGCCTCCAACCCCGGAAACGCCACCACCACCAACCCCAATTCCGGCGCCCGAATCATGGCCCTGCTCGGTGCTCCTTCCTCCAGTCTAGAAATGCCGCCGCAGCCTGAGTTGTCGGTCCCTGGCTCTGGCGGGGTTTCGATGGTGCCCACTCTTCCTATGGGGATTCCACCCTCCCCGGCTCGAATGCCGAGCAATAAGCTGCCGAAGGGCAGGCATTTGATTGGGGATAGTGTGGTTTACGATGTGGATGTTAGGTTGCCCGGGGAGTTTCAGCCGCAGCTGGAAGTTACCCCCATTACTAAGTATGGTTCGGATCCTCAGCTGGTGTTGGGCCGCCAAATTGCGGTGAATAAGTCGTATATATGCTATGGATTGAAGCAGGGGAATATTCGGGTTTTGAATATACACACTGCATTGAGATCTTTGTTTCGAGCTCATACACAGAGGGTCACAGACATGGCTTTCTTTGCCGAGGATGTTCACCTTTTGGCTAGTGTGAGTGTAGAAGGACGGCTTTTTGTGTGGAAAATTTCTGAAGGTCCAGATGAAGAAGGTACGCCACAAATCACAGGAAAGATTGTTGTTGCCATTCAAATAGTTGGTGAGGGGGAAGCTGTTCATCCACGAGTTTGTTGGCACTGTCATAAACAGGAGGTTTTGGTTGTTGGAATTGGGAAGCGTGTTCTACGAATTGATACTACTAAAGTTGCAAAGGGTGAAGTCCCTTCTGCTGAGGATCCTATTAAGTGTCCTGTTGAAAAGCTTGTTGATGGGGTCCAATTTGTTGGTAAACATGATGGAGAGGTGACAGATTTATCAATGTGCCAATGGATGACCACCCGGCTGGTTTCTGCTTCTATGGATGGAACTATAAAGATCTGGGAAGATCGCAAGTCACAACCACTTTTAGTACTGAGACCCTATGATGGCCTTCCAGTTTATTCGTCTACATTTGTGACAGCTCCCAACAGACCTGATCACATCATACTTATAACAGTGGGCCCACTTAATCGGGAAGTGAAGATCTGGTCTTCAGAGAGTGAAGAAGGCTGGCTGCTTCCCAGTGATGCTGAGTCATGGAAATGTACCCAGACCTTAGAGTTAAAGAGCTCTGCTCAACCTCGAGTCGAGGATGCATTCTTTAATCAAGTCATAGCATTGTCACATGCTGGCCTTCTTTTACTTGCAAATGCTAAAAAGAATGCTATATATGCCGTGCACTTAGACTTTGGTGGTGAGCCAGCATCCACACGCATGGATTACATAGCAGAATTTACTGTTACAATGCCTATTTTGAGCTTCACTGGGACAAGTATATCACCTCATGGTGAACAAATCGTTCAGGTGTATTGTGTCCAAACACAAGCCATTCAACAGTATGCTTTGGACTTATCCAAGTGCTTACCTCCCCCGTTGGAGAATTCAGGTTTGGAAAAGTCAGATTCCAGTTTTTCACATGATGCAACCGAGGCGGTGTCAACTAATTCAGCACCAAAACCAACCATACAAGCTACTAGTTCTGAGGGTGCAGCCTTGAGGTATCCTGTGCGCACTGCTTCGGTTGATCCTTCCAATTCAAAAGATATTACCACTTCGTCTATGGAATCAAAACCTGTTGCATCGGCGCCGGAGATTAGTGATGCTGATGCTGTTTTTGTAGCATCACAACCTCTTCCTTTGAGTCCTAGGTTGTCAGGGAAACTTTCTGGTTTAAGAAGCCCGACAGATAGCAGTGATCATGGTGGTGACCAAGTTAATGAGTACTCAGTTGACAGGCAGATGAATACTGCTCGTTCAAACTTGTCTGATATGCCTGCACTCGATGATGATGCAAGGAATGATGAACAGAAAATTGCACAAGACGAAGTATCTAGTGTGCTCAATCCTCCTATTATGTTCAAACATCCAACCCATTTAATAACTCCCTCTGAGATTTTAATGGCTGCTTCATCCTCTGAGAATACAAATCCAATTGACAGCAACACTGAGGGGGATGCAAAAATCCAAGATGTTCTTGTTAACAGTGATGTGGGTAATCCTGAGGTGGAAGTTAAGGTTGTGGGTGAAACCAGATCTACTCAAATCGATGAATTTGGCTCTCAACGAGAACTCCAAAATGTTGTTTCAGAAAATAAGGAGAAGTTCTTTTGCTCCCAGGCATCAGATCTTGGAATTGAGATGGCCCGGGATTGCTGTGCAATATCATCAGAATCTTATATGACAGATGAAGCCCGGCAAGGTGACGGTGCCAGCATGTCAGAACCACTAGCCCAGCCTCATTCTGGAGAGGAAGACCAGGATCTCTCTACAAAAGATGTATCTGGGTCAGCTACTGCCACAACCACATTACAAACACCAAATGCAAAGTCGAGAAAACAGAAATGGAAAAATATGCAAGCATCAGGTCCATCTTCCCCATCACCAGGTCTGCTCAATGCAGTAGAGTCCTCCAATGAAGCAGGTGGGAGTGCAAGTCCCCCCTCCGGAGAAGTTGCATTTCCTCAAATTATGGCCATGCAGGATATGATGAATCAGCTAATGACTATGCAAAAAGAATTGCAAAAGCAGATGACAATGATGGTTACCGTCCCAATTACAAAGGAAGGAAAACGATTAGAGGCGGCTCTCGGTCGGAGTATGGAGAAGGCTGTAAAAGCCAATAATGATGCTTTGTGGGCTCGGTTTCAAGAAGAGAACGCAAAGAATGAGAAATTATTGCGAGACCGCACTCAACAAGTAACTAGTTTGATCAATAACTTTGTGAACAAGGACTTTCCAGTCATGTTAGAGAAAACGGTAAAAAAAGAAATAGCTGCTGTTGGACCAGCTGTTGTCCGTGCAATCACTCCAGCTCTGGAGAAAACAATACCTTTGGCTATATCTGATTGCTTTCAGAGGGGAGTGGGAGATAAAGCAGTTAATCAATTGGAAAAATCAGTTAACTCAAAACTTGAAGCTACTGTATCCAGGCAAATCCAGGCGCAGTTTCAAACATCGGGTAAACAAGCTCTTCAGGATGCTCTCAAGTCTAGCATGGAAGCGTCAGTGGTACCTGCCTTTGAGAAGTCATGTAAAGCCATGTTTGAGCAAGTAGATGCCACATTCCAGAAAGGAATGGTTGAACATGCAACTGTGGCTCAGCAGCATTTTGAGTCTGCACATTCGCCGTTGGCCCATGCTTTAAGGGAAGCTATCAGCTCCGCATCATCAGTGACTCAAACTCTAAGTGGAGAACTGGCTGATGGTCAACGTAAGTTAGTAGCTCTTGCAGCTGCACGGGGAAACTCAAGTGCAGTAAATCCCCTAGTCACTCAACTAACCAATGGACCTTTGGGTGGTCTCCATGAGAAGGTTGAGGTGCCTTTGGATCCAACAAAGGAGCTGTCAAGGTTGGTAACTGAACGCAAGTATGAGGAGGCTTTCACAGGTGCTCTACAAAGAAGTGATGTTGGCATTGTATCATGGTTATGTGCTCAGGTTGATCTTCATGGGATACTGTTGCTGCAACCTGTTCCTCTGAGCCAAGGAGTACTTCTTTCGCTCCTGCAACAGCTGGCCTGTGATATCAACAATGATACACCCCGGAAAGTTGCATGGATGACAGACGTGGCAGCCGCCATAAACCCATCCAACCAGATGATTGCAATGCACGTGAGACCCATCTTTGAGCAAGTTTATCAGATACTGCACCATCAACATAGCTTGCCTACGATCTCCAGTGCCGAGCATGCTAGTCTTCGTCTTCTCATGCATGTCATTAACTCCATGATGATGGCCTGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000932 GO:0000956 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009892 GO:0009933 GO:0009965 GO:0009987 GO:0010014 GO:0010015 GO:0010016 GO:0010033 GO:0010071 GO:0010072 GO:0010154 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0022414 GO:0022622 GO:0023052 GO:0031086 GO:0031087 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0042221 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046700 GO:0046983 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048532 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071704 GO:0090304 GO:0090305 GO:0090421 GO:0097159 GO:0099402 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
Ge1_WD40

Protein Analysis

1394

Amino Acids

151.13

Weight (kDa)

5.67

Isoelectric Point (pI)

45.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ge1_WD40 PF16529 229 - 541 5.2e-19 WD40 region of Ge1, enhancer of mRNA-decapping protein
WD40_WDHD1_1st PF24817 247 - 365 1.8e-06 WDHD1 first WD40 domain
EDC4_C PF21289 1263 - 1366 1.5e-16 Enhancer of mRNA-decapping protein 4, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13290 AT3G13300 AT3G13300 AT3G13300
fragaria_vesca FvH4_3g41972 FvH4_3g41980 FvH4_3g41980 FvH4_3g41980 FvH4_3g41980 FvH4_3g42780 FvH4_3g42790 FvH4_3g42820 FvH4_3g42820 FvH4_3g42820 FvH4_3g42820 FvH4_4g01881 FvH4_7g10420
malus_domestica MD00G1013700.v1.1 MD03G1039800.v1.1 MD11G1041300.v1.1 MD11G1094900.v1.1
prunus_persica Prupe.6G032100_v2.0.a1
pyrus_communis pycom03g03070 pycom11g03500 pycom11g08470 pycom12g06730
rosa_chinensis RchiOBHm_Chr1g0328831 RchiOBHm_Chr4g0386471 RchiOBHm_Chr4g0386481 RchiOBHm_Chr4g0386551 RchiOBHm_Chr4g0386701 RchiOBHm_Chr4g0386801 RchiOBHm_Chr4g0386811 RchiOBHm_Chr4g0386821 RchiOBHm_Chr4g0386921 RchiOBHm_Chr4g0386931 RchiOBHm_Chr4g0386941 RchiOBHm_Chr4g0387211 RchiOBHm_Chr4g0389101 RchiOBHm_Chr4g0389111 RchiOBHm_Chr4g0389201 RchiOBHm_Chr4g0389211 RchiOBHm_Chr4g0389321 RchiOBHm_Chr4g0389331 RchiOBHm_Chr4g0389341 RchiOBHm_Chr4g0389411 RchiOBHm_Chr5g0075171
rosa_laevigata RLG00000009985 RLG00000009990 RLG00000009996 RLG00000009998 RLG00000010136 RLG00000010140 RLG00000036537
rosa_multiflora Rmu_co8461959.1_g000001 Rmu_sc0000013.1_g000017 Rmu_sc0000013.1_g000024 Rmu_sc0001015.1_g000001 Rmu_sc0001395.1_g000004 Rmu_sc0001942.1_g000022 Rmu_sc0002498.1_g000018 Rmu_sc0002498.1_g000020 Rmu_sc0002557.1_g000005 Rmu_sc0002604.1_g000010 Rmu_sc0002604.1_g000011 Rmu_sc0004750.1_g000003 Rmu_sc0006571.1_g000001 Rmu_sc0006571.1_g000014 Rmu_sc0006571.1_g000018 Rmu_sc0008050.1_g000016 Rmu_sc0008066.1_g000009 Rmu_sc0008380.1_g000006 Rmu_sc0008380.1_g000010 Rmu_sc0008380.1_g000012 Rmu_sc0010272.1_g000021 Rmu_sc0010693.1_g000010 Rmu_sc0010693.1_g000012 Rmu_sc0010693.1_g000014 Rmu_sc0010693.1_g000015 Rmu_sc0031790.1_g000003 Rmu_sc0040539.1_g000001 Rmu_ssc0000211.1_g000001 Rmu_ssc0000211.1_g000011 Rmu_ssc0000211.1_g000013
rosa_roxburghii Rroxscaffold_1G00006420 Rroxscaffold_1G00006430 Rroxscaffold_5G00334020 Rroxscaffold_5G00334050 Rroxscaffold_5G00335420
rosa_rugosa Rorug03G0307600 Rorug03G0308600 Rorug03G0322400 Rorug05G0437200 Rorug05G0437200 Rorug05G0437200
rosa_samantha Rh1BG075700 Rh4BG010000 Rh4BG010100 Rh4BG010200 Rh4BG020000 Rh4BG020400 Rh4BG172100 Rh4CG015300 Rh4CG016300 Rh5AG494100 Rh5AG494200 Rh5BG515500 Rh5CG539300 Rh5DG528200
rosa_wichuraiana Rw4G000930 Rw4G001980 Rw4G001990 Rw4G011950 Rw5G045850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1391
AarI CACCTGC 1 cut(s) 2849
AasI GACNNNNNNGTC 1 cut(s) 3176
Acc16I TGCGCA 1 cut(s) 1949
Acc36I ACCTGC 2 cut(s) 2849, 3460
Acc65I GGTACC 1 cut(s) 3448
AccB1I GGYRCC 7 cut(s) 229, 443, 541, 2023, 2645, 3448, 3757
AccI GTMKAC 1 cut(s) 1346
AccIII TCCGGA 1 cut(s) 2882
AclWI GGATC 8 cut(s) 707, 720, 1130, 1143, 1958, 2706, 3761, 3774
AcoI YGGCCR 2 cut(s) 60, 2910
AcsI RAATTY 5 cut(s) 297, 927, 1670, 1819, 2489
AcuI CTGAAG 4 cut(s) 311, 954, 1416, 3395
AcvI CACGTG 1 cut(s) 4051
AcyI GRCGYC 4 cut(s) 5, 230, 444, 2024
AdeI CACNNNGTG 1 cut(s) 322
AfaI GTAC 7 cut(s) 257, 955, 1311, 1486, 2162, 3450, 3930
AhlI ACTAGT 2 cut(s) 1913, 3140
AjiI CACGTC 1 cut(s) 4006
AjnI CCWGG 9 cut(s) 120, 318, 360, 517, 2553, 2694, 2821, 3365, 3375
AjuI GAANNNNNNNTTGG 4 cut(s) 1811, 1843, 3915, 3947
Alw21I GWGCWC 7 cut(s) 472, 835, 1511, 1620, 2280, 3832, 3874
Alw26I GTCTC 4 cut(s) 1309, 3114, 3743, 4048
Alw44I GTGCAC 1 cut(s) 1616
AlwI GGATC 8 cut(s) 707, 720, 1130, 1143, 1958, 2706, 3761, 3774
AlwNI CAGNNNCTG 2 cut(s) 2732, 4084
Ama87I CYCGRG 3 cut(s) 659, 1521, 2583
Aor13HI TCCGGA 1 cut(s) 2882
ApaI GGGCCC 2 cut(s) 207, 1403
ApaLI GTGCAC 1 cut(s) 1616
ApoI RAATTY 5 cut(s) 297, 927, 1670, 1819, 2489
ArsI GACNNNNNNTTYG 4 cut(s) 2750, 2782, 3767, 3799
Asp700I GAANNNNTTC 1 cut(s) 1014
Asp718I GGTACC 1 cut(s) 3448
AspA2I CCTAGG 1 cut(s) 2084
AspLEI GCGC 5 cut(s) 232, 446, 1950, 2026, 3382
AvaI CYCGRG 3 cut(s) 659, 1521, 2583
AvaII GGWCC 6 cut(s) 514, 938, 1174, 2804, 3221, 3725
AvrII CCTAGG 1 cut(s) 2084
AxyI CCTNAGG 2 cut(s) 160, 2439
BaeGI GKGCMC 4 cut(s) 207, 546, 1403, 1620
BalI TGGCCA 2 cut(s) 62, 2912
BamHI GGATCC 3 cut(s) 712, 1135, 3766
BanI GGYRCC 7 cut(s) 229, 443, 541, 2023, 2645, 3448, 3757
BanII GRGCYC 5 cut(s) 207, 835, 1403, 1511, 3079
BauI CACGAG 1 cut(s) 1023
BbrPI CACGTG 1 cut(s) 4051
BbsI GAAGAC 4 cut(s) 1422, 2697, 4127, 4133
Bbv12I GWGCWC 7 cut(s) 472, 835, 1511, 1620, 2280, 3832, 3874
BbvCI CCTCAGC 3 cut(s) 270, 717, 1131
BceAI ACGGC 5 cut(s) 217, 243, 926, 1598, 3550
BcgI CGANNNNNNTGC 2 cut(s) 2210, 2244
BciT130I CCWGG 9 cut(s) 122, 320, 362, 519, 2555, 2696, 2823, 3367, 3377
BciVI GTATCC 3 cut(s) 1950, 3373, 3885
BclI TGATCA 3 cut(s) 1375, 2134, 3147
BcoDI GTCTC 4 cut(s) 1309, 3114, 3743, 4048
BcuI ACTAGT 2 cut(s) 1913, 3140
BfoI RGCGCY 3 cut(s) 233, 447, 2027
BfuAI ACCTGC 2 cut(s) 2849, 3460
BfuI GTATCC 3 cut(s) 1950, 3373, 3885
BglII AGATCT 5 cut(s) 818, 1275, 1422, 2471, 2563
BlnI CCTAGG 1 cut(s) 2084
BlpI GCTNAGC 1 cut(s) 3537
BmcAI AGTACT 3 cut(s) 1311, 2162, 3930
Bme18I GGWCC 6 cut(s) 514, 938, 1174, 2804, 3221, 3725
BmeT110I CYCGRG 3 cut(s) 659, 1521, 2583
BmgBI CACGTC 1 cut(s) 4006
BmrI ACTGGG 2 cut(s) 1455, 1713
BmuI ACTGGG 2 cut(s) 1455, 1713
BpiI GAAGAC 4 cut(s) 1422, 2697, 4127, 4133
BplI GAGNNNNNCTC 2 cut(s) 1498, 1530
BpmI CTGGAG 5 cut(s) 463, 2703, 3231, 3275, 4099
Bpu10I CCTNAGC 4 cut(s) 270, 717, 1131, 3873
Bpu1102I GCTNAGC 1 cut(s) 3537
BpuEI CTTGAG 4 cut(s) 294, 1954, 3407, 3670
Bsa29I ATCGAT 1 cut(s) 2484
BsaAI YACGTR 1 cut(s) 4051
BsaHI GRCGYC 4 cut(s) 5, 230, 444, 2024
BsaI GGTCTC 4 cut(s) 1309, 3114, 3743, 4048
BsaWI WCCGGW 1 cut(s) 2882
BsaXI ACNNNNNCTCC 4 cut(s) 2493, 2523, 2857, 2887
Bse1I ACTGG 9 cut(s) 253, 480, 1334, 1461, 1708, 1845, 3176, 3639, 4116
Bse21I CCTNAGG 2 cut(s) 160, 2439
Bse3DI GCAATG 2 cut(s) 381, 4050
BseAI TCCGGA 1 cut(s) 2882
BseBI CCWGG 9 cut(s) 122, 320, 362, 519, 2555, 2696, 2823, 3367, 3377
BseCI ATCGAT 1 cut(s) 2484
BseMI GCAATG 2 cut(s) 381, 4050
BseNI ACTGG 9 cut(s) 253, 480, 1334, 1461, 1708, 1845, 3176, 3639, 4116
BseRI GAGGAG 2 cut(s) 148, 3827
BseSI GKGCMC 4 cut(s) 207, 546, 1403, 1620
BseYI CCCAGC 2 cut(s) 108, 2670
BsgI GTGCAG 6 cut(s) 1947, 2199, 3540, 3657, 3711, 4070
Bsh1285I CGRYCG 2 cut(s) 338, 3031
BshNI GGYRCC 7 cut(s) 229, 443, 541, 2023, 2645, 3448, 3757
BshVI ATCGAT 1 cut(s) 2484
BsiEI CGRYCG 2 cut(s) 338, 3031
BsiHKAI GWGCWC 7 cut(s) 472, 835, 1511, 1620, 2280, 3832, 3874
BsiHKCI CYCGRG 3 cut(s) 659, 1521, 2583
BslFI GGGAC 5 cut(s) 500, 1106, 1720, 2859, 2972
BsmAI GTCTC 4 cut(s) 1309, 3114, 3743, 4048
BsmFI GGGAC 5 cut(s) 500, 1106, 1720, 2859, 2972
BsmI GAATGC 3 cut(s) 588, 1535, 1606
Bso31I GGTCTC 4 cut(s) 1309, 3114, 3743, 4048
BsoBI CYCGRG 3 cut(s) 659, 1521, 2583
Bsp120I GGGCCC 2 cut(s) 203, 1399
Bsp13I TCCGGA 1 cut(s) 2882
Bsp1720I GCTNAGC 1 cut(s) 3537
BspDI ATCGAT 1 cut(s) 2484
BspEI TCCGGA 1 cut(s) 2882
BspMI ACCTGC 2 cut(s) 2849, 3460
BspPI GGATC 8 cut(s) 707, 720, 1130, 1143, 1958, 2706, 3761, 3774
BspQI GCTCTTC 1 cut(s) 3414
BspT107I GGYRCC 7 cut(s) 229, 443, 541, 2023, 2645, 3448, 3757
BspTNI GGTCTC 4 cut(s) 1309, 3114, 3743, 4048
BsrDI GCAATG 2 cut(s) 381, 4050
BsrI ACTGG 9 cut(s) 253, 480, 1334, 1461, 1708, 1845, 3176, 3639, 4116
BssNI GRCGYC 4 cut(s) 5, 230, 444, 2024
BssSI CACGAG 1 cut(s) 1023
BssT1I CCWWGG 2 cut(s) 2084, 3922
Bst2BI CACGAG 1 cut(s) 1023
Bst2UI CCWGG 9 cut(s) 122, 320, 362, 519, 2555, 2696, 2823, 3367, 3377
Bst6I CTCTTC 4 cut(s) 555, 2076, 3084, 3414
BstACI GRCGYC 4 cut(s) 5, 230, 444, 2024
BstBAI YACGTR 1 cut(s) 4051
BstEII GGTNACC 2 cut(s) 2144, 2980
BstH2I RGCGCY 3 cut(s) 233, 447, 2027
BstHHI GCGC 5 cut(s) 232, 446, 1950, 2026, 3382
BstMAI GTCTC 4 cut(s) 1309, 3114, 3743, 4048
BstMCI CGRYCG 2 cut(s) 338, 3031
BstNI CCWGG 9 cut(s) 122, 320, 362, 519, 2555, 2696, 2823, 3367, 3377
BstNSI RCATGY 5 cut(s) 1568, 2656, 3527, 4130, 4154
BstPI GGTNACC 2 cut(s) 2144, 2980
BstSLI GKGCMC 4 cut(s) 207, 546, 1403, 1620
BstV2I GAAGAC 4 cut(s) 1422, 2697, 4127, 4133
BstX2I RGATCY 9 cut(s) 712, 818, 1135, 1275, 1422, 2471, 2563, 2698, 3766
BstXI CCANNNNNNTGG 4 cut(s) 1184, 1243, 1654, 4171
BstYI RGATCY 9 cut(s) 712, 818, 1135, 1275, 1422, 2471, 2563, 2698, 3766
Bsu15I ATCGAT 1 cut(s) 2484
Bsu36I CCTNAGG 2 cut(s) 160, 2439
BsuI GTATCC 3 cut(s) 1950, 3373, 3885
BsuTUI ATCGAT 1 cut(s) 2484
BtrI CACGTC 1 cut(s) 4006
BtsI GCAGTG 3 cut(s) 807, 1950, 2137
BveI ACCTGC 2 cut(s) 2849, 3460
CaiI CAGNNNCTG 2 cut(s) 2732, 4084
CfoI GCGC 5 cut(s) 232, 446, 1950, 2026, 3382
Cfr9I CCCGGG 2 cut(s) 659, 2583
ClaI ATCGAT 1 cut(s) 2484
CseI GACGC 1 cut(s) 3429
CsiI ACCWGGT 2 cut(s) 318, 2821
Csp6I GTAC 7 cut(s) 256, 954, 1310, 1485, 2161, 3449, 3929
CviQI GTAC 7 cut(s) 256, 954, 1310, 1485, 2161, 3449, 3929
DinI GGCGCC 3 cut(s) 231, 445, 2025
DraI TTTAAA 1 cut(s) 73
DraIII CACNNNGTG 1 cut(s) 322
DrdI GACNNNNNNGTC 1 cut(s) 3176
DseDI GACNNNNNNGTC 1 cut(s) 3176
EaeI YGGCCR 2 cut(s) 60, 2910
Eam1104I CTCTTC 4 cut(s) 555, 2076, 3084, 3414
EarI CTCTTC 4 cut(s) 555, 2076, 3084, 3414
EciI GGCGGA 1 cut(s) 15
Ecl136II GAGCTC 2 cut(s) 833, 1509
Eco130I CCWWGG 2 cut(s) 2084, 3922
Eco24I GRGCYC 5 cut(s) 207, 835, 1403, 1511, 3079
Eco31I GGTCTC 4 cut(s) 1309, 3114, 3743, 4048
Eco32I GATATC 1 cut(s) 3964
Eco47I GGWCC 6 cut(s) 514, 938, 1174, 2804, 3221, 3725
Eco53kI GAGCTC 2 cut(s) 833, 1509
Eco57I CTGAAG 4 cut(s) 311, 954, 1416, 3395
Eco72I CACGTG 1 cut(s) 4051
Eco81I CCTNAGG 2 cut(s) 160, 2439
Eco88I CYCGRG 3 cut(s) 659, 1521, 2583
Eco91I GGTNACC 2 cut(s) 2144, 2980
EcoICRI GAGCTC 2 cut(s) 833, 1509
EcoO109I RGGNCCY 2 cut(s) 124, 204
EcoO65I GGTNACC 2 cut(s) 2144, 2980
EcoRI GAATTC 1 cut(s) 1819
EcoRII CCWGG 9 cut(s) 120, 318, 360, 517, 2553, 2694, 2821, 3365, 3375
EcoRV GATATC 1 cut(s) 3964
EcoT14I CCWWGG 2 cut(s) 2084, 3922
EcoT22I ATGCAT 2 cut(s) 1537, 4152
EcoT38I GRGCYC 5 cut(s) 207, 835, 1403, 1511, 3079
EgeI GGCGCC 3 cut(s) 231, 445, 2025
EheI GGCGCC 3 cut(s) 231, 445, 2025
ErhI CCWWGG 2 cut(s) 2084, 3922
FalI AAGNNNNNCTT 6 cut(s) 897, 929, 2397, 2429, 3915, 3947
FaqI GGGAC 5 cut(s) 500, 1106, 1720, 2859, 2972
FauI CCCGC 2 cut(s) 363, 521
FbaI TGATCA 3 cut(s) 1375, 2134, 3147
FblI GTMKAC 1 cut(s) 1346
FriOI GRGCYC 5 cut(s) 207, 835, 1403, 1511, 3079
FspAI RTGCGCAY 1 cut(s) 1949
FspI TGCGCA 1 cut(s) 1949
GlaI GCGC 5 cut(s) 231, 445, 1949, 2025, 3381
GsaI CCCAGC 2 cut(s) 112, 2674
GsuI CTGGAG 5 cut(s) 463, 2703, 3231, 3275, 4099
HaeII RGCGCY 3 cut(s) 233, 447, 2027
HgaI GACGC 1 cut(s) 3429
HhaI GCGC 5 cut(s) 232, 446, 1950, 2026, 3382
Hin1I GRCGYC 4 cut(s) 5, 230, 444, 2024
Hin6I GCGC 5 cut(s) 230, 444, 1948, 2024, 3380
HinP1I GCGC 5 cut(s) 230, 444, 1948, 2024, 3380
HincII GTYRAC 5 cut(s) 1878, 2170, 2419, 3340, 3647
HindII GTYRAC 5 cut(s) 1878, 2170, 2419, 3340, 3647
HindIII AAGCTT 1 cut(s) 1160
HpaI GTTAAC 2 cut(s) 2419, 3340
HpyCH4IV ACGT 3 cut(s) 3649, 4005, 4050
HpySE526I ACGT 3 cut(s) 3649, 4005, 4050
Hsp92I GRCGYC 4 cut(s) 5, 230, 444, 2024
HspAI GCGC 5 cut(s) 230, 444, 1948, 2024, 3380
KasI GGCGCC 3 cut(s) 229, 443, 2023
Kpn2I TCCGGA 1 cut(s) 2882
KpnI GGTACC 1 cut(s) 3452
Ksp22I TGATCA 3 cut(s) 1375, 2134, 3147
KspAI GTTAAC 2 cut(s) 2419, 3340
LguI GCTCTTC 1 cut(s) 3414
LmnI GCTCC 7 cut(s) 370, 475, 1366, 2555, 3602, 3778, 3945
MabI ACCWGGT 2 cut(s) 318, 2821
MaeII ACGT 3 cut(s) 3649, 4005, 4050
MfeI CAATTG 2 cut(s) 2370, 3323
MflI RGATCY 9 cut(s) 712, 818, 1135, 1275, 1422, 2471, 2563, 2698, 3766
MlsI TGGCCA 2 cut(s) 62, 2912
MluNI TGGCCA 2 cut(s) 62, 2912
Mly113I GGCGCC 3 cut(s) 230, 444, 2024
MlyI GAGTC 6 cut(s) 1481, 1533, 2089, 2852, 3560, 3607
MmeI TCCRAC 8 cut(s) 429, 1045, 1794, 2330, 3011, 3199, 3794, 4053
Mox20I TGGCCA 2 cut(s) 62, 2912
Mph1103I ATGCAT 2 cut(s) 1537, 4152
MroI TCCGGA 1 cut(s) 2882
MroXI GAANNNNTTC 1 cut(s) 1014
MscI TGGCCA 2 cut(s) 62, 2912
MslI CAYNNNNRTG 6 cut(s) 854, 1480, 1652, 2142, 3865, 4169
Msp20I TGGCCA 2 cut(s) 62, 2912
MspA1I CMGCKG 6 cut(s) 334, 679, 721, 3227, 3671, 3952
MunI CAATTG 2 cut(s) 2370, 3323
Mva1269I GAATGC 3 cut(s) 588, 1535, 1606
MvaI CCWGG 9 cut(s) 122, 320, 362, 519, 2555, 2696, 2823, 3367, 3377
NarI GGCGCC 3 cut(s) 230, 444, 2024
NmeAIII GCCGAG 3 cut(s) 612, 895, 4147
NmuCI GTSAC 9 cut(s) 847, 1204, 1293, 1354, 1560, 2144, 2639, 3610, 3706
NsbI TGCGCA 1 cut(s) 1949
NsiI ATGCAT 2 cut(s) 1537, 4152
NspI RCATGY 5 cut(s) 1568, 2656, 3527, 4130, 4154
PaeI GCATGC 1 cut(s) 4130
PaeR7I CTCGAG 1 cut(s) 1521
PaqCI CACCTGC 1 cut(s) 2849
PciSI GCTCTTC 1 cut(s) 3414
PctI GAATGC 3 cut(s) 588, 1535, 1606
PdmI GAANNNNTTC 1 cut(s) 1014
PfeI GAWTC 6 cut(s) 450, 562, 1841, 2003, 2609, 2929
PleI GAGTC 6 cut(s) 1480, 1532, 2088, 2851, 3559, 3607
PluTI GGCGCC 3 cut(s) 233, 447, 2027
PmaCI CACGTG 1 cut(s) 4051
PmlI CACGTG 1 cut(s) 4051
PpsI GAGTC 6 cut(s) 1480, 1532, 2088, 2851, 3559, 3607
Ppu21I YACGTR 1 cut(s) 4051
PsiI TTATAA 1 cut(s) 1391
Psp124BI GAGCTC 2 cut(s) 835, 1511
Psp6I CCWGG 9 cut(s) 120, 318, 360, 517, 2553, 2694, 2821, 3365, 3375
PspCI CACGTG 1 cut(s) 4051
PspEI GGTNACC 2 cut(s) 2144, 2980
PspFI CCCAGC 2 cut(s) 108, 2670
PspGI CCWGG 9 cut(s) 120, 318, 360, 517, 2553, 2694, 2821, 3365, 3375
PspOMI GGGCCC 2 cut(s) 203, 1399
PspXI VCTCGAGB 1 cut(s) 1521
PstNI CAGNNNCTG 2 cut(s) 2732, 4084
PsuI RGATCY 9 cut(s) 712, 818, 1135, 1275, 1422, 2471, 2563, 2698, 3766
PvuII CAGCTG 5 cut(s) 679, 721, 3227, 3671, 3952
RsaI GTAC 7 cut(s) 257, 955, 1311, 1486, 2162, 3450, 3930
RsaNI GTAC 7 cut(s) 256, 954, 1310, 1485, 2161, 3449, 3929
RseI CAYNNNNRTG 6 cut(s) 854, 1480, 1652, 2142, 3865, 4169
SacI GAGCTC 2 cut(s) 835, 1511
SapI GCTCTTC 1 cut(s) 3414
ScaI AGTACT 3 cut(s) 1311, 2162, 3930
SchI GAGTC 6 cut(s) 1481, 1533, 2089, 2852, 3560, 3607
SexAI ACCWGGT 2 cut(s) 318, 2821
SfoI GGCGCC 3 cut(s) 231, 445, 2025
Sfr274I CTCGAG 1 cut(s) 1521
SinI GGWCC 6 cut(s) 514, 938, 1174, 2804, 3221, 3725
SlaI CTCGAG 1 cut(s) 1521
SmaI CCCGGG 2 cut(s) 661, 2585
SmiMI CAYNNNNRTG 6 cut(s) 854, 1480, 1652, 2142, 3865, 4169
SmlI CTYRAG 5 cut(s) 309, 1521, 1933, 3422, 3685
SmoI CTYRAG 5 cut(s) 309, 1521, 1933, 3422, 3685
SpeI ACTAGT 2 cut(s) 1913, 3140
SphI GCATGC 1 cut(s) 4130
SspDI GGCGCC 3 cut(s) 229, 443, 2023
SspI AATATT 1 cut(s) 787
SstI GAGCTC 2 cut(s) 835, 1511
StyI CCWWGG 2 cut(s) 2084, 3922
TaiI ACGT 3 cut(s) 3652, 4008, 4053
TaqI TCGA 9 cut(s) 54, 536, 580, 829, 1522, 1527, 2220, 2484, 2768
TaqII GACCGA 2 cut(s) 502, 3017
TatI WGTACW 3 cut(s) 1309, 2160, 3928
TfiI GAWTC 6 cut(s) 450, 562, 1841, 2003, 2609, 2929
TseFI GTSAC 9 cut(s) 847, 1204, 1293, 1354, 1560, 2144, 2639, 3610, 3706
Tsp45I GTSAC 9 cut(s) 847, 1204, 1293, 1354, 1560, 2144, 2639, 3610, 3706
TspGWI ACGGA 1 cut(s) 3224
TspMI CCCGGG 2 cut(s) 659, 2583
VneI GTGCAC 1 cut(s) 1616
VpaK11BI GGWCC 6 cut(s) 514, 938, 1174, 2804, 3221, 3725
XapI RAATTY 5 cut(s) 297, 927, 1670, 1819, 2489
XbaI TCTAGA 1 cut(s) 484
XceI RCATGY 5 cut(s) 1568, 2656, 3527, 4130, 4154
XcmI CCANNNNNNNNNTGG 6 cut(s) 358, 553, 1029, 1996, 2678, 3727
XhoI CTCGAG 1 cut(s) 1521
XmaI CCCGGG 2 cut(s) 659, 2583
XmaJI CCTAGG 1 cut(s) 2084
XmiI GTMKAC 1 cut(s) 1346
XmnI GAANNNNTTC 1 cut(s) 1014
ZrmI AGTACT 3 cut(s) 1311, 2162, 3930
Zsp2I ATGCAT 2 cut(s) 1537, 4152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.