RchiOBHm_Chr5g0075171

Enhancer of mRNA-decapping protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000324
Physical Location & Seq
Reverse (-)
80987679 .. 80994641
6963 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34991

Sequence Viewer

Length: 3735 bp
ATGGCCCTGCTCGGTGCTCCTTCCTCCGGTCTAGAAATGGCGCCGCAGCCTGAGTTGTCGGTCCCCGGCTCTGGCGGGGTTTCGATGGTGCCCACTCTTCCTATGGGGATTCCACCCTCCCCGGCTCGAATGCCGAGCAATAAGCTGCCGAAGGGCAGGCATTTGATTGGGGATAGCGTGGTTTACGATGTGGATGTTAGGTTGCCCGGGGAGTTTCAGCCGCAGCTGGAAGTTACCCCCATTACTAAGTATGGTTCGGATCCTCAGCTGGTGTTGGGCCGCCAAATTGCGGTGAATAAGTCGTATATATGCTATGGATTGAAGCAGGGGAATATTCGGGTTTTGAATATACACACTGCATTGAGATCTTTGTTTCGAGCTCATACACAGAGGGTCACAGACATGGCTTTCTTTGCCGAGGATGTTCACCTTTTGGCTAGTGTGAGTGTAGAAGGACGGCTTTTTGTGTGGAAAATTTCTGAAGGTCCAGATGAAGAAGGTACGCCACAAATCACAGGAAAGATTGTTATTGCCATTCAAATAGTTGGTGAGGGGGAAGCTGTTCATCCACGAGTTTGTTGGCACTGTCATAAACAGGAGGTTTTGGTTGTTGGAATTGGGAAGCGTGTTCTACGAATTGATACTACTAAAGTTGCAAAGGGTGAAGTCCCTTCTGCTGAGGATCCTATTAAGTGTCCTGTTGAAAAGCTTGTTGATGGGGTCCAATTTGTTGGTAAACATGATGGAGAGGTGACAGATTTATCAATGTGCCAATGGATGACCACCCGGCTGGTTTCTGCTTCTATGGATGGAACTATAAAGATCTGGGAAGATCGCAAGTCACAACCACTTTTAGTACTGAGACCCTATGATGGCCTTCCTGTTTATTCGTCTACATTTGTGACAGCTCCCAACAGACCTGATCACATCATACTTATAACAGTGGGCCCACTTAATCGGGAAGTGAAGATCTGGTCCTCAGAGAGTGAAGAAGGCTGGTTGCTTCCCAGTGATGCTGAGTCATGGAAATGTACCCAGACCTTAGAGTTAAAGAGCTCTGCTCAACCTCGAGTTGAGGATGCATTCTTTAATCAAGTCATAGCATTGTCACAAGCTGGCCTTCTTTTACTTGCAAATGCTAAGAAGAATGCTATATATGCCGTGCACTTAGACTTTGGTGGTGAGCCAGCATCCACACGCATGGATTACATAGCAGAATTTACTGTTACAATGCCTATTTTGAGCTTTACTGGGACAAGTATATCACCTCATGGTGAACAAATCGTTCAGGTGTATTGTGTCCAAACACAAGCCATTCAACAGTATGCTTTGGACTTATCCAAGTGCTTACCTCCCCCATTGGAGAATTCAGGTTTGGAAAAGTCAGATTCCAGTTTTTCACATGATGCAACTGAGGCAGTGTCAACTAATTCAGCACCAAAACCAACCATACAAGCTACTAGTTCTGAGGGTGCAGCCTTGAGGTATCCTGTGCGCACTGCTTCGGTTGATCCTTCCAATTCAAAAGATATTACCACTTCATCTATGGAATCAAAACCTGTTGCATCGGCACCGGAGATTAGTGATGCTGATACTGTTTTTGTAGCATCACAACCTCTTCCTTTGAGTCCTAGGTTGTCAGGGAAACTTTCTGGTTTAAGAAGCCCGACAGGTAGCAGTGATCATGGTGGTGACCAACAAGTTAATGAGTACTCAGTTGACAGGCAGATGAATACTGCTCGTTCAAACTTGTCTGATATGCCTGCACTCGATGATGATGCAAGGAATGATGAACAGAAAATTGCACAAGACGAAGTATCTAGTGTGCTCAATCCTCCTATTATGTTCAAACATCCAACCCATTTAATAACTCCCTCTGAGATTTTAATGGCTGCTTCATCCTCTGAGAATACAAATCCAATTGACAGCAACACTGAGGGGGATGCAAAAATCCAAGATGTTCTTGTTAACAGTGATGTGGGTAATCCTGAGGTGGAAGTTAAGGTTGTGGGTGAAACCAGATCTACTCAAATCGATGAATTTGGCTCTCAACGAGAACTCCAAAATGTTGTTTCAGAAAATAAGGAGAAGTTCTTTTGCTCCCAGGCATCAGATCTTGGAATTGAGATGGCCCGGGATTGCTGTGCAATATCATCAGAATCTTATATTACAGATGAAGCCCGGCAAGGTGATGGTGCCAGCATGTCAGAACCACTAGCCCAGCCTCATTCTGGAGAGGAAGACCAGGATCTCTCTACAAAAGATGTATCTGGGTCAGCTACTGCCACAACCACATCACAAACACCAAATGCGAAGTCGAGAAAACAGAAATGGAAAAATATGCAAGCATCAGGTCCATCTGCCCCATCACCAGGTGTGCTCAATGTAGTAGAGTCCTCCAATGAAGCAGGTGGGAGTGCAAGTCCCCCCTCCGGAGAAGCTGCATTTCCTCAAATTATGGCCATGCAGGATATGATGAATCAGCTAATGACTATGCAAAAAGAATTGCAAAAGCAGATGACAATGATGGTTACCGTCCCAATTACAAAGGAAGGCAAACGATTAGAGGCGGCTCTCGGTCGGAGTATGGAGAAGGCTGTAAAAGCCAATAATGATGCTTTGTGGGCTCGGTTTCAAGAAGAGAACGCAAAGAATGAGAAATTATTGCGAGACCGCACTCAACAAGTAACTAGTTTGATCAATAACTTTGTGAACAAGGACTTTCCAGTCATGTTAGAGAAAACGGTAAAAAAAGAAATAGCTGCTGTTGGACCAGCTGTTGTTCGTGCAATCACTCCAGCTATGGAGAAAACAATACCTTTGGCTATATCTGATTGCTTTCAGAGGGGAGTGGGAGATAAAGCAGTTAATCAATTGGAAAAATCAGTCAACTCAAAACTTGAAGCTACCGTATCCAGGCAAATCCAGGCGCAGTTTCAAACATCGGGTAAACAAGCTCTTCAGGATGCTCTCAAGTCTAGCATGGAAGCTTCAGTGGTACCTGCCTTTGAGAAGTCATGTAAAGCCATGTTTGAGCAAGTAGATGCCACATTCCAGAAAGGAATGGTTGAACATGCAACTGTGGCTCAGCAGCATTTTGAGTCTGCACATTCGCCGTTGGCTCATGCTTTAAGGGAAGCTATCAGCTCCGCATCATCAGTGACTCAAACTCTAAGTGGAGAACTGGCTGATGGTCAACGTAAGTTAGTAGCTCTTGCAGCTGCACGGGGAAACTCAAGTGCGGTAAATCCCCTAGTCACTCAACTAACCAATGGACCTTTGGGTGGTCTCCATGAGAAGGTTGAGGTGCCTTTGGATCCAACAAAGGAGCTGTCAAGGTTGGTAACTGAACGCAAGTATGAGGAGGCTTTCACTGGTGCTCTACAAAGAAGTGATGTTGGCATTGTATCATGGTTATGTGCTCAGGTTGATCTTCATGGGATACTGTTGCTGCAACCTGTTCCTCTGAGCCAAGGAGTACTTCTTTCGCTCCTGCAACAGCTGGCCTGTGATATCAACAATGATACACCCCGGAAAGTTGCATGGATGACAGACGTGGCAGCCGCCATAAACCCATCCAACCAGATGATTGCAATGCACGTGAGACCCATCTTTGAGCAAGTTTATCAGATACTGCACCATCAACATAGCTTGCCTACGATCTCCAGTGCCGAGCATGCTAGTCTTCGTCTTCTCATGCATGTCATTAACTCCATGATGATGGCCTGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000932 GO:0000956 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009892 GO:0009933 GO:0009965 GO:0009987 GO:0010014 GO:0010015 GO:0010016 GO:0010033 GO:0010071 GO:0010072 GO:0010154 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0022414 GO:0022622 GO:0023052 GO:0031086 GO:0031087 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0042221 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046700 GO:0046983 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048532 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071704 GO:0090304 GO:0090305 GO:0090421 GO:0097159 GO:0099402 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
Ge1_WD40

Protein Analysis

1244

Amino Acids

134.79

Weight (kDa)

5.43

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ge1_WD40 PF16529 78 - 390 3.7e-19 WD40 region of Ge1, enhancer of mRNA-decapping protein
WD40_WDHD1_1st PF24817 96 - 214 2e-06 WDHD1 first WD40 domain
EDC4_C PF21289 1113 - 1216 1.3e-16 Enhancer of mRNA-decapping protein 4, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13290 AT3G13300 AT3G13300 AT3G13300
fragaria_vesca FvH4_3g41972 FvH4_3g41980 FvH4_3g41980 FvH4_3g41980 FvH4_3g41980 FvH4_3g42780 FvH4_3g42790 FvH4_3g42820 FvH4_3g42820 FvH4_3g42820 FvH4_3g42820 FvH4_4g01881 FvH4_7g10420
malus_domestica MD00G1013700.v1.1 MD03G1039800.v1.1 MD11G1041300.v1.1 MD11G1094900.v1.1
prunus_persica Prupe.6G032100_v2.0.a1
pyrus_communis pycom03g03070 pycom11g03500 pycom11g08470 pycom12g06730
rosa_chinensis RchiOBHm_Chr1g0328831 RchiOBHm_Chr4g0386471 RchiOBHm_Chr4g0386481 RchiOBHm_Chr4g0386551 RchiOBHm_Chr4g0386701 RchiOBHm_Chr4g0386801 RchiOBHm_Chr4g0386811 RchiOBHm_Chr4g0386821 RchiOBHm_Chr4g0386921 RchiOBHm_Chr4g0386931 RchiOBHm_Chr4g0386941 RchiOBHm_Chr4g0387211 RchiOBHm_Chr4g0389101 RchiOBHm_Chr4g0389111 RchiOBHm_Chr4g0389201 RchiOBHm_Chr4g0389211 RchiOBHm_Chr4g0389321 RchiOBHm_Chr4g0389331 RchiOBHm_Chr4g0389341 RchiOBHm_Chr4g0389411 RchiOBHm_Chr5g0075171
rosa_laevigata RLG00000009985 RLG00000009990 RLG00000009996 RLG00000009998 RLG00000010136 RLG00000010140 RLG00000036537
rosa_multiflora Rmu_co8461959.1_g000001 Rmu_sc0000013.1_g000017 Rmu_sc0000013.1_g000024 Rmu_sc0001015.1_g000001 Rmu_sc0001395.1_g000004 Rmu_sc0001942.1_g000022 Rmu_sc0002498.1_g000018 Rmu_sc0002498.1_g000020 Rmu_sc0002557.1_g000005 Rmu_sc0002604.1_g000010 Rmu_sc0002604.1_g000011 Rmu_sc0004750.1_g000003 Rmu_sc0006571.1_g000001 Rmu_sc0006571.1_g000014 Rmu_sc0006571.1_g000018 Rmu_sc0008050.1_g000016 Rmu_sc0008066.1_g000009 Rmu_sc0008380.1_g000006 Rmu_sc0008380.1_g000010 Rmu_sc0008380.1_g000012 Rmu_sc0010272.1_g000021 Rmu_sc0010693.1_g000010 Rmu_sc0010693.1_g000012 Rmu_sc0010693.1_g000014 Rmu_sc0010693.1_g000015 Rmu_sc0031790.1_g000003 Rmu_sc0040539.1_g000001 Rmu_ssc0000211.1_g000001 Rmu_ssc0000211.1_g000011 Rmu_ssc0000211.1_g000013
rosa_roxburghii Rroxscaffold_1G00006420 Rroxscaffold_1G00006430 Rroxscaffold_5G00334020 Rroxscaffold_5G00334050 Rroxscaffold_5G00335420
rosa_rugosa Rorug03G0307600 Rorug03G0308600 Rorug03G0322400 Rorug05G0437200 Rorug05G0437200 Rorug05G0437200
rosa_samantha Rh1BG075700 Rh4BG010000 Rh4BG010100 Rh4BG010200 Rh4BG020000 Rh4BG020400 Rh4BG172100 Rh4CG015300 Rh4CG016300 Rh5AG494100 Rh5AG494200 Rh5BG515500 Rh5CG539300 Rh5DG528200
rosa_wichuraiana Rw4G000930 Rw4G001980 Rw4G001990 Rw4G011950 Rw5G045850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 938
AarI CACCTGC 1 cut(s) 2399
AasI GACNNNNNNGTC 1 cut(s) 2726
Acc16I TGCGCA 1 cut(s) 1496
Acc36I ACCTGC 2 cut(s) 2399, 3010
Acc65I GGTACC 1 cut(s) 2998
AccB1I GGYRCC 6 cut(s) 40, 88, 1570, 2195, 2998, 3307
AccI GTMKAC 1 cut(s) 893
AccIII TCCGGA 1 cut(s) 2432
AclWI GGATC 8 cut(s) 254, 267, 677, 690, 1505, 2256, 3311, 3324
AcoI YGGCCR 1 cut(s) 2460
AcsI RAATTY 4 cut(s) 474, 1217, 1366, 2039
AcuI CTGAAG 3 cut(s) 501, 2945, 2976
AcvI CACGTG 1 cut(s) 3601
AcyI GRCGYC 1 cut(s) 41
AdeI CACNNNGTG 1 cut(s) 2375
AfaI GTAC 6 cut(s) 502, 858, 1033, 1712, 3000, 3480
AhlI ACTAGT 2 cut(s) 1460, 2690
AjiI CACGTC 1 cut(s) 3556
AjnI CCWGG 5 cut(s) 2103, 2244, 2371, 2915, 2925
AjuI GAANNNNNNNTTGG 4 cut(s) 1358, 1390, 3465, 3497
Alw21I GWGCWC 8 cut(s) 19, 382, 1058, 1167, 1830, 2382, 3382, 3424
Alw26I GTCTC 4 cut(s) 856, 2664, 3293, 3598
Alw44I GTGCAC 1 cut(s) 1163
AlwI GGATC 8 cut(s) 254, 267, 677, 690, 1505, 2256, 3311, 3324
AlwNI CAGNNNCTG 2 cut(s) 2282, 3634
Ama87I CYCGRG 3 cut(s) 206, 1068, 2133
Aor13HI TCCGGA 1 cut(s) 2432
AoxI GGCC 9 cut(s) 3, 277, 874, 946, 1117, 2130, 2460, 3504, 3723
ApaI GGGCCC 1 cut(s) 950
ApaLI GTGCAC 1 cut(s) 1163
ApoI RAATTY 4 cut(s) 474, 1217, 1366, 2039
ArsI GACNNNNNNTTYG 4 cut(s) 2300, 2332, 3317, 3349
Asp700I GAANNNNTTC 1 cut(s) 561
Asp718I GGTACC 1 cut(s) 2998
AspA2I CCTAGG 1 cut(s) 1631
AspLEI GCGC 3 cut(s) 43, 1497, 2932
AsuC2I CCSGG 9 cut(s) 66, 122, 207, 208, 787, 2134, 2135, 2182, 3532
AvaI CYCGRG 3 cut(s) 206, 1068, 2133
AvaII GGWCC 7 cut(s) 61, 485, 721, 975, 2354, 2771, 3275
AvrII CCTAGG 1 cut(s) 1631
AxyI CCTNAGG 1 cut(s) 1989
BaeGI GKGCMC 3 cut(s) 93, 950, 1167
BalI TGGCCA 1 cut(s) 2462
BamHI GGATCC 3 cut(s) 259, 682, 3316
BanI GGYRCC 6 cut(s) 40, 88, 1570, 2195, 2998, 3307
BanII GRGCYC 4 cut(s) 382, 950, 1058, 2629
BauI CACGAG 1 cut(s) 570
BbrPI CACGTG 1 cut(s) 3601
BbsI GAAGAC 3 cut(s) 2247, 3677, 3683
Bbv12I GWGCWC 8 cut(s) 19, 382, 1058, 1167, 1830, 2382, 3382, 3424
BbvCI CCTCAGC 2 cut(s) 264, 678
BceAI ACGGC 3 cut(s) 473, 1145, 3100
BcgI CGANNNNNNTGC 2 cut(s) 1760, 1794
BciT130I CCWGG 5 cut(s) 2105, 2246, 2373, 2917, 2927
BciVI GTATCC 3 cut(s) 1497, 2923, 3435
BclI TGATCA 3 cut(s) 922, 1681, 2697
BcnI CCSGG 9 cut(s) 66, 122, 207, 208, 787, 2134, 2135, 2182, 3532
BcoDI GTCTC 4 cut(s) 856, 2664, 3293, 3598
BcuI ACTAGT 2 cut(s) 1460, 2690
BfoI RGCGCY 1 cut(s) 44
BfuAI ACCTGC 2 cut(s) 2399, 3010
BfuI GTATCC 3 cut(s) 1497, 2923, 3435
BglII AGATCT 5 cut(s) 365, 822, 969, 2021, 2113
BlnI CCTAGG 1 cut(s) 1631
BlpI GCTNAGC 1 cut(s) 3087
BmcAI AGTACT 3 cut(s) 858, 1712, 3480
Bme18I GGWCC 7 cut(s) 61, 485, 721, 975, 2354, 2771, 3275
BmeT110I CYCGRG 3 cut(s) 206, 1068, 2133
BmgBI CACGTC 1 cut(s) 3556
BmrI ACTGGG 2 cut(s) 1002, 1260
BmuI ACTGGG 2 cut(s) 1002, 1260
BpiI GAAGAC 3 cut(s) 2247, 3677, 3683
BplI GAGNNNNNCTC 2 cut(s) 1045, 1077
BpmI CTGGAG 3 cut(s) 2253, 2781, 3649
Bpu10I CCTNAGC 3 cut(s) 264, 678, 3423
Bpu1102I GCTNAGC 1 cut(s) 3087
BpuEI CTTGAG 3 cut(s) 1501, 2957, 3220
BpuMI CCSGG 9 cut(s) 66, 122, 207, 208, 787, 2134, 2135, 2182, 3532
Bsa29I ATCGAT 1 cut(s) 2034
BsaAI YACGTR 1 cut(s) 3601
BsaHI GRCGYC 1 cut(s) 41
BsaI GGTCTC 4 cut(s) 856, 2664, 3293, 3598
BsaWI WCCGGW 3 cut(s) 26, 1573, 2432
BsaXI ACNNNNNCTCC 4 cut(s) 2043, 2073, 2407, 2437
Bse1I ACTGG 7 cut(s) 1008, 1255, 1392, 2726, 3189, 3379, 3666
Bse21I CCTNAGG 1 cut(s) 1989
Bse3DI GCAATG 1 cut(s) 3600
BseAI TCCGGA 1 cut(s) 2432
BseBI CCWGG 5 cut(s) 2105, 2246, 2373, 2917, 2927
BseCI ATCGAT 1 cut(s) 2034
BseMI GCAATG 1 cut(s) 3600
BseNI ACTGG 7 cut(s) 1008, 1255, 1392, 2726, 3189, 3379, 3666
BseRI GAGGAG 1 cut(s) 3377
BseSI GKGCMC 3 cut(s) 93, 950, 1167
BseYI CCCAGC 1 cut(s) 2220
BsgI GTGCAG 5 cut(s) 1494, 1749, 3090, 3207, 3620
Bsh1285I CGRYCG 1 cut(s) 2581
BshFI GGCC 9 cut(s) 5, 279, 876, 948, 1119, 2132, 2462, 3506, 3725
BshNI GGYRCC 6 cut(s) 40, 88, 1570, 2195, 2998, 3307
BshVI ATCGAT 1 cut(s) 2034
BsiEI CGRYCG 1 cut(s) 2581
BsiHKAI GWGCWC 8 cut(s) 19, 382, 1058, 1167, 1830, 2382, 3382, 3424
BsiHKCI CYCGRG 3 cut(s) 206, 1068, 2133
BslFI GGGAC 5 cut(s) 47, 653, 1267, 2409, 2522
BsmAI GTCTC 4 cut(s) 856, 2664, 3293, 3598
BsmFI GGGAC 5 cut(s) 47, 653, 1267, 2409, 2522
BsmI GAATGC 3 cut(s) 135, 1082, 1153
BsnI GGCC 9 cut(s) 5, 279, 876, 948, 1119, 2132, 2462, 3506, 3725
Bso31I GGTCTC 4 cut(s) 856, 2664, 3293, 3598
BsoBI CYCGRG 3 cut(s) 206, 1068, 2133
Bsp120I GGGCCC 1 cut(s) 946
Bsp13I TCCGGA 1 cut(s) 2432
Bsp1720I GCTNAGC 1 cut(s) 3087
BspANI GGCC 9 cut(s) 5, 279, 876, 948, 1119, 2132, 2462, 3506, 3725
BspDI ATCGAT 1 cut(s) 2034
BspEI TCCGGA 1 cut(s) 2432
BspMI ACCTGC 2 cut(s) 2399, 3010
BspPI GGATC 8 cut(s) 254, 267, 677, 690, 1505, 2256, 3311, 3324
BspQI GCTCTTC 1 cut(s) 2964
BspT107I GGYRCC 6 cut(s) 40, 88, 1570, 2195, 2998, 3307
BspTNI GGTCTC 4 cut(s) 856, 2664, 3293, 3598
BsrDI GCAATG 1 cut(s) 3600
BsrI ACTGG 7 cut(s) 1008, 1255, 1392, 2726, 3189, 3379, 3666
BssNI GRCGYC 1 cut(s) 41
BssSI CACGAG 1 cut(s) 570
BssT1I CCWWGG 2 cut(s) 1631, 3472
Bst2BI CACGAG 1 cut(s) 570
Bst2UI CCWGG 5 cut(s) 2105, 2246, 2373, 2917, 2927
Bst6I CTCTTC 4 cut(s) 102, 1623, 2634, 2964
BstACI GRCGYC 1 cut(s) 41
BstBAI YACGTR 1 cut(s) 3601
BstC8I GCNNGC 9 cut(s) 158, 1117, 1188, 1764, 2200, 2346, 3504, 3653, 3678
BstEII GGTNACC 2 cut(s) 1691, 2530
BstH2I RGCGCY 1 cut(s) 44
BstHHI GCGC 3 cut(s) 43, 1497, 2932
BstMAI GTCTC 4 cut(s) 856, 2664, 3293, 3598
BstMCI CGRYCG 1 cut(s) 2581
BstMWI GCNNNNNNNGC 8 cut(s) 413, 1157, 1415, 2603, 2624, 3083, 3218, 3677
BstNI CCWGG 5 cut(s) 2105, 2246, 2373, 2917, 2927
BstNSI RCATGY 4 cut(s) 2206, 3077, 3680, 3704
BstPI GGTNACC 2 cut(s) 1691, 2530
BstSLI GKGCMC 3 cut(s) 93, 950, 1167
BstV2I GAAGAC 3 cut(s) 2247, 3677, 3683
BstX2I RGATCY 9 cut(s) 259, 365, 682, 822, 969, 2021, 2113, 2248, 3316
BstXI CCANNNNNNTGG 4 cut(s) 731, 790, 1201, 3721
BstYI RGATCY 9 cut(s) 259, 365, 682, 822, 969, 2021, 2113, 2248, 3316
Bsu15I ATCGAT 1 cut(s) 2034
Bsu36I CCTNAGG 1 cut(s) 1989
BsuI GTATCC 3 cut(s) 1497, 2923, 3435
BsuRI GGCC 9 cut(s) 5, 279, 876, 948, 1119, 2132, 2462, 3506, 3725
BsuTUI ATCGAT 1 cut(s) 2034
BtrI CACGTC 1 cut(s) 3556
BtsI GCAGTG 4 cut(s) 354, 1425, 1497, 1684
BveI ACCTGC 2 cut(s) 2399, 3010
Cac8I GCNNGC 9 cut(s) 158, 1117, 1188, 1764, 2200, 2346, 3504, 3653, 3678
CaiI CAGNNNCTG 2 cut(s) 2282, 3634
CfoI GCGC 3 cut(s) 43, 1497, 2932
Cfr9I CCCGGG 2 cut(s) 206, 2133
ClaI ATCGAT 1 cut(s) 2034
CsiI ACCWGGT 1 cut(s) 2371
Csp6I GTAC 6 cut(s) 501, 857, 1032, 1711, 2999, 3479
CviQI GTAC 6 cut(s) 501, 857, 1032, 1711, 2999, 3479
DinI GGCGCC 1 cut(s) 42
DraIII CACNNNGTG 1 cut(s) 2375
DrdI GACNNNNNNGTC 1 cut(s) 2726
DseDI GACNNNNNNGTC 1 cut(s) 2726
EaeI YGGCCR 1 cut(s) 2460
Eam1104I CTCTTC 4 cut(s) 102, 1623, 2634, 2964
EarI CTCTTC 4 cut(s) 102, 1623, 2634, 2964
Ecl136II GAGCTC 2 cut(s) 380, 1056
Eco130I CCWWGG 2 cut(s) 1631, 3472
Eco24I GRGCYC 4 cut(s) 382, 950, 1058, 2629
Eco31I GGTCTC 4 cut(s) 856, 2664, 3293, 3598
Eco32I GATATC 1 cut(s) 3514
Eco47I GGWCC 7 cut(s) 61, 485, 721, 975, 2354, 2771, 3275
Eco53kI GAGCTC 2 cut(s) 380, 1056
Eco57I CTGAAG 3 cut(s) 501, 2945, 2976
Eco72I CACGTG 1 cut(s) 3601
Eco81I CCTNAGG 1 cut(s) 1989
Eco88I CYCGRG 3 cut(s) 206, 1068, 2133
Eco91I GGTNACC 2 cut(s) 1691, 2530
EcoICRI GAGCTC 2 cut(s) 380, 1056
EcoO65I GGTNACC 2 cut(s) 1691, 2530
EcoRI GAATTC 1 cut(s) 1366
EcoRII CCWGG 5 cut(s) 2103, 2244, 2371, 2915, 2925
EcoRV GATATC 1 cut(s) 3514
EcoT14I CCWWGG 2 cut(s) 1631, 3472
EcoT22I ATGCAT 2 cut(s) 1084, 3702
EcoT38I GRGCYC 4 cut(s) 382, 950, 1058, 2629
EgeI GGCGCC 1 cut(s) 42
EheI GGCGCC 1 cut(s) 42
ErhI CCWWGG 2 cut(s) 1631, 3472
FalI AAGNNNNNCTT 8 cut(s) 444, 476, 1104, 1136, 1947, 1979, 3465, 3497
FaqI GGGAC 5 cut(s) 47, 653, 1267, 2409, 2522
FauI CCCGC 1 cut(s) 68
FbaI TGATCA 3 cut(s) 922, 1681, 2697
FblI GTMKAC 1 cut(s) 893
FriOI GRGCYC 4 cut(s) 382, 950, 1058, 2629
FspAI RTGCGCAY 1 cut(s) 1496
FspI TGCGCA 1 cut(s) 1496
GlaI GCGC 3 cut(s) 42, 1496, 2931
GsaI CCCAGC 1 cut(s) 2224
GsuI CTGGAG 3 cut(s) 2253, 2781, 3649
HaeII RGCGCY 1 cut(s) 44
HaeIII GGCC 9 cut(s) 5, 279, 876, 948, 1119, 2132, 2462, 3506, 3725
HhaI GCGC 3 cut(s) 43, 1497, 2932
Hin1I GRCGYC 1 cut(s) 41
Hin6I GCGC 3 cut(s) 41, 1495, 2930
HinP1I GCGC 3 cut(s) 41, 1495, 2930
HincII GTYRAC 5 cut(s) 1425, 1720, 1969, 2890, 3197
HindII GTYRAC 5 cut(s) 1425, 1720, 1969, 2890, 3197
HindIII AAGCTT 2 cut(s) 707, 2988
HpaI GTTAAC 1 cut(s) 1969
HpyCH4IV ACGT 3 cut(s) 3199, 3555, 3600
HpyF10VI GCNNNNNNNGC 8 cut(s) 413, 1157, 1415, 2603, 2624, 3083, 3218, 3677
HpySE526I ACGT 3 cut(s) 3199, 3555, 3600
Hsp92I GRCGYC 1 cut(s) 41
HspAI GCGC 3 cut(s) 41, 1495, 2930
KasI GGCGCC 1 cut(s) 40
Kpn2I TCCGGA 1 cut(s) 2432
KpnI GGTACC 1 cut(s) 3002
Ksp22I TGATCA 3 cut(s) 922, 1681, 2697
KspAI GTTAAC 1 cut(s) 1969
LguI GCTCTTC 1 cut(s) 2964
LmnI GCTCC 6 cut(s) 22, 913, 2105, 3152, 3328, 3495
MabI ACCWGGT 1 cut(s) 2371
MaeII ACGT 3 cut(s) 3199, 3555, 3600
MfeI CAATTG 2 cut(s) 1920, 2873
MflI RGATCY 9 cut(s) 259, 365, 682, 822, 969, 2021, 2113, 2248, 3316
MlsI TGGCCA 1 cut(s) 2462
MluNI TGGCCA 1 cut(s) 2462
Mly113I GGCGCC 1 cut(s) 41
MlyI GAGTC 5 cut(s) 1028, 1636, 2402, 3110, 3157
MmeI TCCRAC 6 cut(s) 592, 1880, 2561, 2749, 3344, 3603
Mox20I TGGCCA 1 cut(s) 2462
Mph1103I ATGCAT 2 cut(s) 1084, 3702
MroI TCCGGA 1 cut(s) 2432
MroXI GAANNNNTTC 1 cut(s) 561
MscI TGGCCA 1 cut(s) 2462
MslI CAYNNNNRTG 6 cut(s) 401, 1027, 1199, 1689, 3415, 3719
Msp20I TGGCCA 1 cut(s) 2462
MspA1I CMGCKG 5 cut(s) 226, 268, 2777, 3221, 3502
MunI CAATTG 2 cut(s) 1920, 2873
Mva1269I GAATGC 3 cut(s) 135, 1082, 1153
MvaI CCWGG 5 cut(s) 2105, 2246, 2373, 2917, 2927
MwoI GCNNNNNNNGC 8 cut(s) 413, 1157, 1415, 2603, 2624, 3083, 3218, 3677
NarI GGCGCC 1 cut(s) 41
NciI CCSGG 9 cut(s) 66, 122, 207, 208, 787, 2134, 2135, 2182, 3532
NmeAIII GCCGAG 3 cut(s) 159, 442, 3697
NmuCI GTSAC 8 cut(s) 394, 751, 840, 901, 1107, 1691, 3160, 3256
NsbI TGCGCA 1 cut(s) 1496
NsiI ATGCAT 2 cut(s) 1084, 3702
NspI RCATGY 4 cut(s) 2206, 3077, 3680, 3704
PaeI GCATGC 1 cut(s) 3680
PaeR7I CTCGAG 1 cut(s) 1068
PaqCI CACCTGC 1 cut(s) 2399
PciSI GCTCTTC 1 cut(s) 2964
PctI GAATGC 3 cut(s) 135, 1082, 1153
PdmI GAANNNNTTC 1 cut(s) 561
PfeI GAWTC 5 cut(s) 109, 1388, 1550, 2159, 2479
PleI GAGTC 5 cut(s) 1027, 1635, 2401, 3109, 3157
PluTI GGCGCC 1 cut(s) 44
PmaCI CACGTG 1 cut(s) 3601
PmlI CACGTG 1 cut(s) 3601
PpsI GAGTC 5 cut(s) 1027, 1635, 2401, 3109, 3157
Ppu21I YACGTR 1 cut(s) 3601
PsiI TTATAA 1 cut(s) 938
Psp124BI GAGCTC 2 cut(s) 382, 1058
Psp6I CCWGG 5 cut(s) 2103, 2244, 2371, 2915, 2925
PspCI CACGTG 1 cut(s) 3601
PspEI GGTNACC 2 cut(s) 1691, 2530
PspFI CCCAGC 1 cut(s) 2220
PspGI CCWGG 5 cut(s) 2103, 2244, 2371, 2915, 2925
PspOMI GGGCCC 1 cut(s) 946
PspXI VCTCGAGB 1 cut(s) 1068
PstNI CAGNNNCTG 2 cut(s) 2282, 3634
PsuI RGATCY 9 cut(s) 259, 365, 682, 822, 969, 2021, 2113, 2248, 3316
PvuII CAGCTG 5 cut(s) 226, 268, 2777, 3221, 3502
RsaI GTAC 6 cut(s) 502, 858, 1033, 1712, 3000, 3480
RsaNI GTAC 6 cut(s) 501, 857, 1032, 1711, 2999, 3479
RseI CAYNNNNRTG 6 cut(s) 401, 1027, 1199, 1689, 3415, 3719
SacI GAGCTC 2 cut(s) 382, 1058
SapI GCTCTTC 1 cut(s) 2964
ScaI AGTACT 3 cut(s) 858, 1712, 3480
SchI GAGTC 5 cut(s) 1028, 1636, 2402, 3110, 3157
SexAI ACCWGGT 1 cut(s) 2371
SfoI GGCGCC 1 cut(s) 42
Sfr274I CTCGAG 1 cut(s) 1068
SinI GGWCC 7 cut(s) 61, 485, 721, 975, 2354, 2771, 3275
SlaI CTCGAG 1 cut(s) 1068
SmaI CCCGGG 2 cut(s) 208, 2135
SmiMI CAYNNNNRTG 6 cut(s) 401, 1027, 1199, 1689, 3415, 3719
SmlI CTYRAG 4 cut(s) 1068, 1480, 2972, 3235
SmoI CTYRAG 4 cut(s) 1068, 1480, 2972, 3235
SpeI ACTAGT 2 cut(s) 1460, 2690
SphI GCATGC 1 cut(s) 3680
SspDI GGCGCC 1 cut(s) 40
SspI AATATT 1 cut(s) 334
SstI GAGCTC 2 cut(s) 382, 1058
StyI CCWWGG 2 cut(s) 1631, 3472
TaiI ACGT 3 cut(s) 3202, 3558, 3603
TaqI TCGA 7 cut(s) 83, 127, 376, 1069, 1770, 2034, 2318
TaqII GACCGA 2 cut(s) 49, 2567
TatI WGTACW 3 cut(s) 856, 1710, 3478
TauI GCSGC 5 cut(s) 46, 223, 282, 2573, 3566
TfiI GAWTC 5 cut(s) 109, 1388, 1550, 2159, 2479
TseFI GTSAC 8 cut(s) 394, 751, 840, 901, 1107, 1691, 3160, 3256
Tsp45I GTSAC 8 cut(s) 394, 751, 840, 901, 1107, 1691, 3160, 3256
TspMI CCCGGG 2 cut(s) 206, 2133
VneI GTGCAC 1 cut(s) 1163
VpaK11BI GGWCC 7 cut(s) 61, 485, 721, 975, 2354, 2771, 3275
XapI RAATTY 4 cut(s) 474, 1217, 1366, 2039
XbaI TCTAGA 1 cut(s) 31
XceI RCATGY 4 cut(s) 2206, 3077, 3680, 3704
XcmI CCANNNNNNNNNTGG 5 cut(s) 100, 576, 1543, 2228, 3277
XhoI CTCGAG 1 cut(s) 1068
XmaI CCCGGG 2 cut(s) 206, 2133
XmaJI CCTAGG 1 cut(s) 1631
XmiI GTMKAC 1 cut(s) 893
XmnI GAANNNNTTC 1 cut(s) 561
ZrmI AGTACT 3 cut(s) 858, 1712, 3480
Zsp2I ATGCAT 2 cut(s) 1084, 3702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.