FvH4_2g20000

Protein DA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000415
Physical Location & Seq
Reverse (-)
16847052 .. 16847616
565 bp
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UTR
Exon/CDS
Intron
FvH4_2g20000.t1

Sequence Viewer

Length: 414 bp
ATGGGTACGCTAGCTTGTCTCAGAAGAATATATGTTCCCAAGAAGCCATCTGCAGTGGCTCGGCGAACAATTTCAGGCATAGTAGTGGTGTTAGTTTTGTTTGGGAAACCGGATGTGGACACAGGCCGAGGTCGTTCATTTGATTTGGAAGAAAGGGTTAAAGAAGGCATTTGCGAAGTGATGGCATACATGTGGATGGAGTGGTTTTGTTCTAATGGTTTTGATTCATCTTACAAAACCAGCAAGGAAGCTCAAAATACAAGAGACTTGAAAGACCATCTAGCACATAAGATGGAACGCAGTAAGAGCATTGCTTATGGCCAAGGGTTTAGAGATGCTATCAAGGCTGTAGCCAAATTTGGCCTTACAACTACACTGGATCATATCGTCAAAGAGGGAAGTCCCCCTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

138

Amino Acids

15.35

Weight (kDa)

9.25

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 48 - 130 1.4e-17 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 387
AcoI YGGCCR 1 cut(s) 319
AcsI RAATTY 1 cut(s) 356
AfaI GTAC 1 cut(s) 7
AflIII ACRYGT 1 cut(s) 189
AgsI TTSAA 1 cut(s) 271
AluBI AGCT 2 cut(s) 14, 251
AluI AGCT 2 cut(s) 14, 251
Alw26I GTCTC 2 cut(s) 23, 258
AlwI GGATC 1 cut(s) 387
AoxI GGCC 3 cut(s) 124, 319, 361
ApoI RAATTY 1 cut(s) 356
Asp700I GAANNNNTTC 1 cut(s) 70
AsuNHI GCTAGC 1 cut(s) 10
BalI TGGCCA 1 cut(s) 321
BccI CCATC 5 cut(s) 55, 175, 190, 285, 286
BcoDI GTCTC 2 cut(s) 23, 258
BfaI CTAG 2 cut(s) 11, 281
BfmI CTRYAG 2 cut(s) 51, 348
BmsI GCATC 1 cut(s) 325
BmtI GCTAGC 1 cut(s) 14
BsaJI CCNNGG 2 cut(s) 127, 322
BsaWI WCCGGW 1 cut(s) 109
Bse1I ACTGG 1 cut(s) 381
Bse3DI GCAATG 1 cut(s) 309
BseDI CCNNGG 2 cut(s) 127, 322
BseGI GGATG 2 cut(s) 118, 201
BseMI GCAATG 1 cut(s) 309
BseMII CTCAG 1 cut(s) 34
BseNI ACTGG 1 cut(s) 381
BshFI GGCC 3 cut(s) 126, 321, 363
BsiSI CCGG 1 cut(s) 110
BslFI GGGAC 1 cut(s) 387
BsmAI GTCTC 2 cut(s) 23, 258
BsmFI GGGAC 1 cut(s) 387
BsnI GGCC 3 cut(s) 126, 321, 363
Bsp143I GATC 1 cut(s) 379
BspANI GGCC 3 cut(s) 126, 321, 363
BspCNI CTCAG 1 cut(s) 33
BspMAI CTGCAG 1 cut(s) 55
BspOI GCTAGC 1 cut(s) 14
BspPI GGATC 1 cut(s) 387
BsrDI GCAATG 1 cut(s) 309
BsrI ACTGG 1 cut(s) 381
BssECI CCNNGG 2 cut(s) 127, 322
BssMI GATC 1 cut(s) 379
BssT1I CCWWGG 1 cut(s) 322
BstC8I GCNNGC 1 cut(s) 12
BstDEI CTNAG 1 cut(s) 20
BstF5I GGATG 2 cut(s) 118, 201
BstKTI GATC 1 cut(s) 382
BstMAI GTCTC 2 cut(s) 23, 258
BstMBI GATC 1 cut(s) 379
BstMWI GCNNNNNNNGC 2 cut(s) 306, 344
BstNSI RCATGY 1 cut(s) 193
BstSFI CTRYAG 2 cut(s) 51, 348
BsuRI GGCC 3 cut(s) 126, 321, 363
BtsCI GGATG 2 cut(s) 118, 201
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 2 cut(s) 60, 374
Cac8I GCNNGC 1 cut(s) 12
Csp6I GTAC 1 cut(s) 6
CviAII CATG 1 cut(s) 190
CviJI RGCY 9 cut(s) 14, 46, 59, 126, 251, 321, 347, 353, 363
CviKI_1 RGCY 9 cut(s) 14, 46, 59, 126, 251, 321, 347, 353, 363
CviQI GTAC 1 cut(s) 6
DdeI CTNAG 1 cut(s) 20
DpnI GATC 1 cut(s) 381
DpnII GATC 1 cut(s) 379
EaeI YGGCCR 1 cut(s) 319
Eco130I CCWWGG 1 cut(s) 322
EcoT14I CCWWGG 1 cut(s) 322
ErhI CCWWGG 1 cut(s) 322
FaeI CATG 1 cut(s) 193
FaiI YATR 8 cut(s) 31, 33, 80, 187, 191, 288, 318, 384
FalI AAGNNNNNCTT 1 cut(s) 391
FaqI GGGAC 1 cut(s) 387
FatI CATG 1 cut(s) 189
FokI GGATG 2 cut(s) 125, 208
FspBI CTAG 2 cut(s) 11, 281
HaeIII GGCC 3 cut(s) 126, 321, 363
HapII CCGG 1 cut(s) 110
Hin1II CATG 1 cut(s) 193
HinfI GANTC 1 cut(s) 224
HpaII CCGG 1 cut(s) 110
Hpy166II GTNNAC 1 cut(s) 118
Hpy188I TCNGA 1 cut(s) 23
Hpy8I GTNNAC 1 cut(s) 118
HpyAV CCTTC 1 cut(s) 158
HpyCH4V TGCA 1 cut(s) 53
HpyF10VI GCNNNNNNNGC 2 cut(s) 306, 344
HpyF3I CTNAG 1 cut(s) 20
Hsp92II CATG 1 cut(s) 193
Kzo9I GATC 1 cut(s) 379
LpnPI CCDG 5 cut(s) 60, 108, 123, 253, 362
LweI GCATC 1 cut(s) 325
MaeI CTAG 2 cut(s) 11, 281
MalI GATC 1 cut(s) 381
MboI GATC 1 cut(s) 379
MboII GAAGA 2 cut(s) 36, 161
MlsI TGGCCA 1 cut(s) 321
MluCI AATT 2 cut(s) 69, 356
MluNI TGGCCA 1 cut(s) 321
MnlI CCTC 2 cut(s) 122, 388
Mox20I TGGCCA 1 cut(s) 321
MroXI GAANNNNTTC 1 cut(s) 70
MscI TGGCCA 1 cut(s) 321
MseI TTAA 1 cut(s) 159
MslI CAYNNNNRTG 3 cut(s) 83, 190, 194
Msp20I TGGCCA 1 cut(s) 321
MspI CCGG 1 cut(s) 110
MwoI GCNNNNNNNGC 2 cut(s) 306, 344
NdeII GATC 1 cut(s) 379
NheI GCTAGC 1 cut(s) 10
NlaIII CATG 1 cut(s) 193
NmeAIII GCCGAG 2 cut(s) 40, 152
NspI RCATGY 1 cut(s) 193
PciI ACATGT 1 cut(s) 189
PdmI GAANNNNTTC 1 cut(s) 70
PfeI GAWTC 1 cut(s) 224
PscI ACATGT 1 cut(s) 189
PstI CTGCAG 1 cut(s) 55
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
RseI CAYNNNNRTG 3 cut(s) 83, 190, 194
SaqAI TTAA 1 cut(s) 159
Sau3AI GATC 1 cut(s) 379
SetI ASST 3 cut(s) 16, 133, 253
SfaNI GCATC 1 cut(s) 325
SfcI CTRYAG 2 cut(s) 51, 348
SmiMI CAYNNNNRTG 3 cut(s) 83, 190, 194
Sse9I AATT 2 cut(s) 69, 356
SspMI CTAG 2 cut(s) 11, 281
StyI CCWWGG 1 cut(s) 322
TasI AATT 2 cut(s) 69, 356
TfiI GAWTC 1 cut(s) 224
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 2 cut(s) 60, 381
TspDTI ATGAA 2 cut(s) 126, 216
TspRI CASTG 2 cut(s) 60, 381
XapI RAATTY 1 cut(s) 356
XceI RCATGY 1 cut(s) 193
XmnI GAANNNNTTC 1 cut(s) 70
XspI CTAG 2 cut(s) 11, 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.