MD10G1023300.v1.1

DA1-related 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000415
Physical Location & Seq
Reverse (-)
2908945 .. 2911069
2125 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1023300.v1.1.491

Sequence Viewer

Length: 717 bp
ATGATTCCATTTTGGAATTTTAATTATTACAAGATACATGAATCTGATGGAACTCCAAGGTGTAATACGTGTGACAGATTCAAGACAACAGGGCAGAATGAATATGTGAATCTTGGAAATAACCAGCAGCTTTGCTCGGAATGTTTTTCCACTGCTATCTTGCATCCAAGTAAATGCAAACGTCTTATTGAAAATGTGCGCAAATTTTATAAAAAGTTAGGCCTCCAAGTGGATAAAAAAATTCCTATTTTATTGATCGACGACGATGAACTGAGAAGAATTCACCCAAATGAACAGATGCTGAATGTGGTTGGTGTTACCACACACCCTCCTTACACTGTCATGACGGTATATGTCGCTTACTTTTGGGTTGTAACAGATTTCAAAATGTTCAAGGAAAGGGTGATGATCGGTGCAACTTTGGCGCATGAGATGATGCATGCATGGTTAGCTCTTCAAGTTTCAGAAGGCATTTGTGAAGTGATGGCACACATATGGTTGACATCATGCTGTAAGAAGGGTTTGGAGTCATACAACACCAGGCACGGGCATGCTGTATTTTTGGAAAACTTGCAAAAGTATGCAACCTACAAAATAGAATCCAATGTGGACAAAAATTATGGACAAGGATTTAGAGAGGCTAACATGGCGGTAACTAAATTTGGACTCCGAGAAACCATAGATTATATCATTCGAAATAAAACTCTCCCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0032182 GO:0043130
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like LIM

Protein Analysis

239

Amino Acids

27.78

Weight (kDa)

8.82

Isoelectric Point (pI)

36.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 134 - 231 2.6e-18 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 210
Acc16I TGCGCA 1 cut(s) 200
AciI CCGC 1 cut(s) 650
AcsI RAATTY 5 cut(s) 16, 203, 240, 279, 659
AfiI CCNNNNNNNGG 2 cut(s) 229, 546
AflIII ACRYGT 1 cut(s) 68
AgsI TTSAA 5 cut(s) 82, 191, 385, 394, 458
AjnI CCWGG 1 cut(s) 539
AluBI AGCT 2 cut(s) 130, 452
AluI AGCT 2 cut(s) 130, 452
AlwNI CAGNNNCTG 1 cut(s) 301
AoxI GGCC 1 cut(s) 220
ApeKI GCWGC 1 cut(s) 127
ApoI RAATTY 5 cut(s) 16, 203, 240, 279, 659
AspLEI GCGC 2 cut(s) 201, 427
AsuHPI GGTGA 2 cut(s) 275, 415
AsuII TTCGAA 1 cut(s) 694
BbvI GCAGC 1 cut(s) 139
BccI CCATC 2 cut(s) 41, 478
BciT130I CCWGG 1 cut(s) 541
BisI GCNGC 1 cut(s) 128
BlsI GCNGC 1 cut(s) 129
Bme1390I CCNGG 1 cut(s) 541
BmrFI CCNGG 1 cut(s) 541
BmsI GCATC 3 cut(s) 172, 288, 426
Bpu14I TTCGAA 1 cut(s) 694
BsaAI YACGTR 1 cut(s) 69
BsaJI CCNNGG 1 cut(s) 56
BsaXI ACNNNNNCTCC 2 cut(s) 313, 343
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 546
BseBI CCWGG 1 cut(s) 541
BseDI CCNNGG 1 cut(s) 56
BseGI GGATG 1 cut(s) 163
BseLI CCNNNNNNNGG 2 cut(s) 229, 546
BseMII CTCAG 1 cut(s) 263
BseXI GCAGC 1 cut(s) 139
BshFI GGCC 1 cut(s) 222
BslI CCNNNNNNNGG 2 cut(s) 229, 546
BsnI GGCC 1 cut(s) 222
Bsp119I TTCGAA 1 cut(s) 694
Bsp143I GATC 2 cut(s) 255, 408
BspACI CCGC 1 cut(s) 650
BspANI GGCC 1 cut(s) 222
BspCNI CTCAG 1 cut(s) 264
BspHI TCATGA 1 cut(s) 342
BspQI GCTCTTC 1 cut(s) 459
BspT104I TTCGAA 1 cut(s) 694
BssECI CCNNGG 1 cut(s) 56
BssMI GATC 2 cut(s) 255, 408
BssT1I CCWWGG 1 cut(s) 56
Bst2UI CCWGG 1 cut(s) 541
Bst4CI ACNGT 2 cut(s) 340, 349
Bst6I CTCTTC 1 cut(s) 459
BstBAI YACGTR 1 cut(s) 69
BstBI TTCGAA 1 cut(s) 694
BstC8I GCNNGC 2 cut(s) 441, 552
BstDEI CTNAG 1 cut(s) 272
BstF5I GGATG 1 cut(s) 163
BstHHI GCGC 2 cut(s) 201, 427
BstKTI GATC 2 cut(s) 258, 411
BstMBI GATC 2 cut(s) 255, 408
BstMWI GCNNNNNNNGC 3 cut(s) 422, 449, 647
BstNI CCWGG 1 cut(s) 541
BstNSI RCATGY 2 cut(s) 443, 554
BstSCI CCNGG 1 cut(s) 539
BstV1I GCAGC 1 cut(s) 139
BsuRI GGCC 1 cut(s) 222
BtsCI GGATG 1 cut(s) 163
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 2 cut(s) 150, 336
Cac8I GCNNGC 2 cut(s) 441, 552
CaiI CAGNNNCTG 1 cut(s) 301
CciI TCATGA 1 cut(s) 342
CfoI GCGC 2 cut(s) 201, 427
CviAII CATG 8 cut(s) 38, 343, 428, 440, 444, 507, 551, 646
CviJI RGCY 4 cut(s) 130, 222, 452, 641
CviKI_1 RGCY 4 cut(s) 130, 222, 452, 641
DdeI CTNAG 1 cut(s) 272
DpnI GATC 2 cut(s) 257, 410
DpnII GATC 2 cut(s) 255, 408
Eam1104I CTCTTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 459
Eco130I CCWWGG 1 cut(s) 56
Eco147I AGGCCT 1 cut(s) 222
EcoRI GAATTC 1 cut(s) 279
EcoRII CCWGG 1 cut(s) 539
EcoT14I CCWWGG 1 cut(s) 56
EcoT22I ATGCAT 2 cut(s) 441, 445
ErhI CCWWGG 1 cut(s) 56
FaeI CATG 8 cut(s) 41, 346, 431, 443, 447, 510, 554, 649
FatI CATG 8 cut(s) 37, 342, 427, 439, 443, 506, 550, 645
FauNDI CATATG 1 cut(s) 494
Fnu4HI GCNGC 1 cut(s) 128
FokI GGATG 1 cut(s) 150
Fsp4HI GCNGC 1 cut(s) 128
FspI TGCGCA 1 cut(s) 200
GlaI GCGC 2 cut(s) 200, 426
GluI GCNGC 1 cut(s) 128
HaeIII GGCC 1 cut(s) 222
HhaI GCGC 2 cut(s) 201, 427
Hin1II CATG 8 cut(s) 41, 346, 431, 443, 447, 510, 554, 649
Hin6I GCGC 2 cut(s) 199, 425
HinP1I GCGC 2 cut(s) 199, 425
HincII GTYRAC 1 cut(s) 501
HindII GTYRAC 1 cut(s) 501
HinfI GANTC 7 cut(s) 4, 41, 78, 109, 527, 599, 666
HphI GGTGA 2 cut(s) 275, 415
Hpy166II GTNNAC 2 cut(s) 501, 610
Hpy188I TCNGA 4 cut(s) 46, 139, 466, 671
Hpy188III TCNNGA 2 cut(s) 82, 343
Hpy8I GTNNAC 2 cut(s) 501, 610
Hpy99I CGWCG 2 cut(s) 263, 266
HpyAV CCTTC 2 cut(s) 461, 511
HpyCH4III ACNGT 2 cut(s) 340, 349
HpyCH4IV ACGT 2 cut(s) 68, 181
HpyCH4V TGCA 7 cut(s) 163, 177, 416, 439, 443, 574, 584
HpyF10VI GCNNNNNNNGC 3 cut(s) 422, 449, 647
HpyF3I CTNAG 1 cut(s) 272
HpySE526I ACGT 2 cut(s) 68, 181
Hsp92II CATG 8 cut(s) 41, 346, 431, 443, 447, 510, 554, 649
HspAI GCGC 2 cut(s) 199, 425
Kzo9I GATC 2 cut(s) 255, 408
LguI GCTCTTC 1 cut(s) 459
LpnPI CCDG 4 cut(s) 75, 137, 526, 553
Lsp1109I GCAGC 1 cut(s) 139
LweI GCATC 3 cut(s) 172, 288, 426
MaeII ACGT 2 cut(s) 68, 181
MaeIII GTNAC 4 cut(s) 71, 316, 373, 652
MalI GATC 2 cut(s) 257, 410
MboI GATC 2 cut(s) 255, 408
MboII GAAGA 2 cut(s) 288, 446
MluCI AATT 7 cut(s) 16, 22, 203, 240, 279, 616, 659
MlyI GAGTC 2 cut(s) 536, 660
MnlI CCTC 3 cut(s) 233, 339, 631
Mph1103I ATGCAT 2 cut(s) 441, 445
MseI TTAA 1 cut(s) 21
MslI CAYNNNNRTG 4 cut(s) 288, 341, 493, 549
MspR9I CCNGG 1 cut(s) 541
MvaI CCWGG 1 cut(s) 541
MwoI GCNNNNNNNGC 3 cut(s) 422, 449, 647
NdeI CATATG 1 cut(s) 494
NdeII GATC 2 cut(s) 255, 408
NlaIII CATG 8 cut(s) 41, 346, 431, 443, 447, 510, 554, 649
NmuCI GTSAC 1 cut(s) 71
NsbI TGCGCA 1 cut(s) 200
NsiI ATGCAT 2 cut(s) 441, 445
NspI RCATGY 2 cut(s) 443, 554
NspV TTCGAA 1 cut(s) 694
PaeI GCATGC 2 cut(s) 443, 554
PagI TCATGA 1 cut(s) 342
PceI AGGCCT 1 cut(s) 222
PciSI GCTCTTC 1 cut(s) 459
PfeI GAWTC 5 cut(s) 4, 41, 78, 109, 599
PkrI GCNGC 1 cut(s) 129
PleI GAGTC 2 cut(s) 535, 660
PpsI GAGTC 2 cut(s) 535, 660
Ppu21I YACGTR 1 cut(s) 69
PsiI TTATAA 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 539
PspGI CCWGG 1 cut(s) 539
PstNI CAGNNNCTG 1 cut(s) 301
RseI CAYNNNNRTG 4 cut(s) 288, 341, 493, 549
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 1 cut(s) 21
SatI GCNGC 1 cut(s) 128
Sau3AI GATC 2 cut(s) 255, 408
SchI GAGTC 2 cut(s) 536, 660
ScrFI CCNGG 1 cut(s) 541
SetI ASST 6 cut(s) 62, 71, 132, 184, 454, 590
SfaNI GCATC 3 cut(s) 172, 288, 426
SfuI TTCGAA 1 cut(s) 694
SmiMI CAYNNNNRTG 4 cut(s) 288, 341, 493, 549
SphI GCATGC 2 cut(s) 443, 554
Sse9I AATT 7 cut(s) 16, 22, 203, 240, 279, 616, 659
SseBI AGGCCT 1 cut(s) 222
SsiI CCGC 1 cut(s) 650
StuI AGGCCT 1 cut(s) 222
StyD4I CCNGG 1 cut(s) 539
StyI CCWWGG 1 cut(s) 56
TaaI ACNGT 2 cut(s) 340, 349
TaiI ACGT 2 cut(s) 71, 184
TaqI TCGA 3 cut(s) 258, 694, 712
TasI AATT 7 cut(s) 16, 22, 203, 240, 279, 616, 659
TfiI GAWTC 5 cut(s) 4, 41, 78, 109, 599
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TscAI CASTG 2 cut(s) 157, 343
TseFI GTSAC 1 cut(s) 71
TseI GCWGC 1 cut(s) 127
Tsp45I GTSAC 1 cut(s) 71
TspDTI ATGAA 4 cut(s) 54, 114, 282, 306
TspRI CASTG 2 cut(s) 157, 343
XapI RAATTY 5 cut(s) 16, 203, 240, 279, 659
XceI RCATGY 2 cut(s) 443, 554
Zsp2I ATGCAT 2 cut(s) 441, 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.